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Southeastern European Medical Journal : SEEMEDJ
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  This is an Open Access Journal Open Access journal
ISSN (Online) 2459-9484
Published by Josip Juraj Strossmayer University of Osijek Homepage  [3 journals]
  • Editorial

    • Authors: Ines Drenjančević
      PubDate: 2022-11-27
      Issue No: Vol. 6, No. 2 (2022)
  • Do Water Molecules Displaced by Hydrophobic Interactions Stabilize
           Antigen-Antibody Binding'

    • Authors: Peter Nemeth*
      Pages: 1 - 19
      Abstract: BACKGROUND: Antigen-antibody reactions are a special field of molecular interactions. The physico-chemical nature of antigen-antibody binding and ligand-induced changes in the fine molecular structures of antigens during immunocomplex formation are less studied. However, these changes in the molecular appearance are extremely important for further molecular recognition. The major aim of this study is to clarify the physico-chemical modification of the antigen/hapten during immunobinding using model experiments. METHODS: An appropriate model system was designed for our investigations: fluorescein-iso-thiocyanate (FITC, isomer I) was used as the antigen (hapten), and its interactions with a specific antibody (monoclonal anti-FITC IgG1) were analyzed using spectrophotometry, different spectrofluorimetric methods and fluorescence polarization, and Fourier-transform infrared spectroscopic methods. RESULTS: Fluorescent polarization and infrared spectroscopic measurements detected a local decrease in the hydration degree in the submolecular area of the specific ligand between the small antigen (hapten) molecule and the hypervariable region of the specific IgG1, causing “rigidization” of molecular movements. Changes in hydration modified the molecular microenvironment, allowing them to influence further functions of both immunoglobulins and the antigen. CONCLUSION: Hydrophobic interactions with exclusion of water molecules around the binding sites seem to be thermodynamically strong enough for stable molecular binding without a covalent chemical interaction between the antigen and the antibody. The results of this study, together with data obtained in previous research, help understand the molecular dynamics of the antigen-antibody reaction better.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.257
      Issue No: Vol. 6, No. 2 (2022)
  • Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive
           Compounds Targeted to Blood Coagulation System

    • Authors: Eldar Iskandarov*, Volodymyr Gryshchuk, Oleh Platonov, Yevhenii Kucheriavyi, Oleksandr Slominskyi, Yevhenii Stohnii, Volodymyr Vartanov, Volodymyr Chernyshenko
      Pages: 20 - 31
      Abstract: Introduction. The performed research focused on a search for new biologically active compounds acting on blood coagulation system proteins and cells. To achieve this goal, we fractionated Vipera berus berus snake venom and studied the action of the separated fractions on human blood plasma, fibrinogen, platelets or red cells. Methods. Crude venom was fractionated using ion-exchange chromatography. Protein composition of fractions was studied using SDS-PAGE. The ability of fractions to prolong or initiate blood plasma clotting was studied using the prothrombin time test with thromboplastin. Fibrinogen-specific proteases were detected using enzyme-electrophoresis. Action on red cells was estimated using the hemolysis test. Aggregometry was used for the detection of action on platelets. All experiments in this study were performed in vitro. Results. We obtained fractions containing phospholipase and a protease that is able to hydrolyze fibrinogen, leading to the loss of its ability to polymerize and to maintain platelet aggregation. Conclusion. Further purification and study of these components can be a promising research direction for biotechnological as well as for biomedical use.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.256
      Issue No: Vol. 6, No. 2 (2022)
  • The Effect of Submaximal Exercise on Cutaneous Blood Flow,
           Thermoregulation and Recovery Hemodynamics Following Endurance Exercise

    • Authors: Nejka Potočnik*
      Pages: 32 - 43
      Abstract: Aim. There are numerous reports of attenuation of cutaneous blood flow (CBF) during the recovery period after a single bout of exercise, but no one has investigated the CBF response to consecutive short-lasting aerobic exercise (SLAE) sessions following exhaustive endurance exercise (EEE) on  daily basis, although this is a commonly used training regime in recreational athletes and could cause a cumulative increase in CBF that may be important for wound healing. This study examined the effects of EEE on forearm skin blood flow (LDF), cutaneous vascular conductance (CVC), and mean arterial pressure (MAP) in response to SLAE sessions performed for 7 days after EEE, as well as the correlation between cutaneous blood flow and indices associated with heart rate (HR) to examine the role of thermoregulation in post-exercise HR regulation. Methods. In 19 recreational runners, LDF, MAP, HR, heart rate recovery (HRR), and HR variability indices (lnRMSSD, lnHF, and lnLF/ HF) were measured after SLAE in the form of submaximal graded cycling performed on consecutive days after EEE in the form of a half marathon and compared with baseline values before EEE. A significant time effect was observed for all measured parameters throughout the study period. Results. Postexercise LDF increased 24 hours after EEE compared with baseline (77.814 AU compared with 54.712 AU). Postexercise hypotension was significantly more pronounced immediately after EEE compared with baseline (88.95.3 mmHg compared with 99.33.2 mmHg). However, postexercise CVC showed a progressive increase compared with baseline both immediately and 24 hours after EEE (0.53 0.07 AU/mmHg, 0.66 0.09 AU/mmHg compared with 0.4 0.09 AU/mmHg). A small negative correlation between LDF and HRR was observed throughout the experimental protocol. Conclusion. These results suggest that EEE strongly influences cutaneous and systemic hemodynamics and cardiac autonomic response during recovery after SLAE. Our most important finding was that EEE improved cutaneous perfusion 24 hours after completion of EEE, which may be important for wound healing. The results of our study are potentially applicable for patients with chronic wounds who should be encouraged to exercise moderately on daily basis and to include endurance exercises occasionally, as this strategy potentiates postexercise cutaneous perfusion.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.261
      Issue No: Vol. 6, No. 2 (2022)
  • Trefoil Factor 3 Protein and Sepsis

    • Authors: Iva Bazina*, Mirela Baus Lončar
      Pages: 44 - 53
      Abstract: Sepsis is one of the most common causes of death in hospitalized patients. Disruption of intestinal barrier homeostasis is one of its main hallmarks. Trefoil factor family proteins are known for their role in protecting and repairing the intestinal mucosa. It has been repeatedly shown that the TFF3 protein is involved in maintaining the intestinal barrier. For that reason, it has been studied in the search for objective measures to predict the onset or outcome of sepsis. Several studies have been performed on rodent sepsis models and on sepsis patients, both children and adults. From the limited research available to date, it appears that TFF3 is involved in the pathogenesis of sepsis, but the exact mechanism is not yet clear. Its potential as a sepsis biomarker has so far been low, but more extensive studies on its role in predicting disease severity and outcome, as well as organ dysfunction, may lead to finding specific patient groups or sepsis stages for which it would be suitable.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.253
      Issue No: Vol. 6, No. 2 (2022)
  • Comorbidity of Somatic Illnesses on People With Treated Mental Disorders
           – A New Challenge in Medicine

    • Authors: Romana Marušić, Adriana Levaković, Dunja Degmečić, Tatjana Bačun*
      Pages: 54 - 66
      Abstract: Aim: Comorbidities pose a major challenge for 21st century medicine. The mutual pathophysiological effect of one disease on another can significantly affect their course and prognosis. The aims of this study were to examine the frequency of comorbidities and the most common psychiatric and somatic comorbidities and to determine the difference in the incidence of certain diseases by gender and age. Methods: Data were recorded in several groups: demographic characteristics, psychiatric and somatic diagnoses classified according to gender, age, and the legally determined ability to work, and correlations of somatic and psychiatric diagnoses. Results: The most common psychiatric diagnoses in men were post-traumatic stress disorder (PTSD) (25%) and alcoholism (23%), while in women these were recurrent depressive disorder (19%) and psychosis (10%). A statistically significant difference was found in the incidence of alcoholism and PTSD, which are more common in men than in women. The most common somatic diseases in both sexes were arterial hypertension (M = 33%, F = 46%) and diabetes mellitus (M = 18%, F = 32%). Statistically significant differences were found in the frequency of hypertension (p = 0.03) and epilepsy (p = 0.002), which are more common in men. The ratio alcoholism-hypertension (p = 0.03), alcoholism-diabetes (p < 0.0001), alcoholism-COPD (p < 0.001) was statistically significant. Conclusion: It is extremely important to improve the multidisciplinary approach and cooperation in treatment in order to reduce the number of hospitalizations, emergency interventions and suicides and to improve the patients’ quality of life and life expectancy.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.248
      Issue No: Vol. 6, No. 2 (2022)
  • Vitamin D Deficiency Among Medical Students in Osijek, Croatia

    • Authors: Stipe Vidović*, Željko Debeljak, Tatjana Bačun, Sven Viland, Milorad Zjalić, Marija Heffer
      Pages: 67 - 74
      Abstract: Abstract Aim: This study aimed to evaluate serum levels of 25-OH D3 (calcidiol) among students of the Faculty of Medicine Osijek, Croatia, thereby determining to what extent vitamin D deficiency is present.  Methods: The present cross-sectional analysis was based on data collected from 60 participants. Blood sampling was done in March 2021. Concentrations of 25-OH D3 were measured using LC/MS-MS procedure on Shimadzu LCMS 8050 and RECIPE kit for serum levels of 25-OH-D3.  Results: The study was conducted on a sample of 60 respondents aged 19 to 28, of whom 16 were men and 44 were women. All subjects had a 25-OH D3 deficiency (<20 ng/ml), while 11 had an extreme deficiency. Mean 25-OH D3 concentration was 11.36 ng/ml, ranging from 7.70 ng/ml to 16.70 ng/ml. There was no statistical significance of 25-OH D3 concentration levels between the sexes (Student’s t-test, P>0.05).  Conclusion: Vitamin D deficiency was detected in all subjects. In addition to the results of several other studies conducted worldwide that evaluated vitamin D status among medical students, this study further highlights the problem affecting this student subgroup.    
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.258
      Issue No: Vol. 6, No. 2 (2022)
  • Iron Chelation Therapy in COVID-19 Infection

    • Authors: Irena Krajina Kmoniček*, Anja Tomić, Josip Kocur
      Pages: 75 - 84
      Abstract:   The recent outbreak of corona virus and coronavirus disease (COVID-19) caused by the SARS-CoV-2 virus is a global concern. Despite efforts to clarify the physiology and potential therapy, specific guidelines for the treatment of COVID-19 disease have yet to be established, and many therapeutic options are under investigation. Accumulating evidence suggests that dysregulation of iron homeostasis contributes significantly to the pathogenesis of COVID-19 through its toxic effects by the formation of reactive oxygen species (ROS). This review focuses on summarizing the available literature and relevant studies conducted to date on the possible therapeutic effects of iron chelation therapy in the treatment of COVID-19 disease. Scientific databases (PubMed, Scopus, Google Scholar) were searched for relevant articles using the following keywords: COVID-19, SARS-CoV-2, coronavirus, clinical management, iron chelators/chelation. Research articles, reviews, research letters, case reports, and commentaries were considered. Although there is ample evidence of the potential beneficial effects of using iron chelators as adjuvant treatment in COVID-19, further research on this topic is needed.
      PubDate: 2022-11-27
      DOI: 10.26332/seemedj.v6i2.246
      Issue No: Vol. 6, No. 2 (2022)
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