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Florea : Jurnal Biologi dan Pembelajarannya
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  This is an Open Access Journal Open Access journal
ISSN (Print) 2335-6102 - ISSN (Online) 2502-0404
Published by Universitas PGRI Madiun Homepage  [4 journals]
  • Efektivitas Jumlah Ikan Nila (Oreochromis niloticus) Terhadap Pertumbuhan
           Tanaman Sawi Pakcoy (Brassica rapa L.) pada Sistem Akuaponik

    • Authors: Diah Karunia Binawati, Rikza Fardatus Sabila
      Pages: 74 - 79
      Abstract: Sawi pakcoy (B. rapa L) is one of the most well known kinds of vegetables in Indonesia. Farming innovation that can be utilized is hydroponics innovation. In hydroponics frameworks, creature discharges are given to plants to be separated into nitrates and nitrites through regular cycles and can be used by plants as supplements from fish squander. Tilapia (O. niloticus) is one of the freshwater fish products that are most popular by different gatherings. The motivation behind this review was to decide the impact of the quantity of tilapia (O. niloticus) on the development of mustard greens and the treatment of the ideal number of tilapia (O. niloticus) on the development of mustard greens (B. rapa L) utilizing hydroponics framework. Randomized square plan was utilized in this review with three medicines and nine replications. Information handling utilized one way ANOVA factual examination, if critical, further Duncan Numerous Reach Test (DMRT) was done. The aftereffects of the investigation of the quantity of leaves, wet weight and complete chlorophyll content of the Pakcoy Mustard (B. rapa L) plant with the ANOVA test showed critical numbers (P <0.005), accordingly the quantity of tilapia (O. niloticus) influenced the development of the Pakcoy Sawi (B. rapa L) plant. ). The aftereffects of the treatment of the quantity of tilapia (O. niloticus) 80 fish (P3) gave ideal outcomes on the development of the mustard greens (B. rapa L) with hydroponics framework.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.15185
      Issue No: Vol. 9, No. 2 (2022)
  • Pengaruh Variasi Suhu Sebelum dan Setelah Penyimpanan terhadap Stabilitas
           Fisik dan Kontaminasi Bakteri Suspensi Antasida Generik

    • Authors: Rica Laily Istighfarin, Siti Nur Azizah, Asa Falahi, Amaliyah Nurul Hidayah
      Pages: 80 - 85
      Abstract: The purpose of this study was to determine the effect of temperature variations before and after storage on physical stability and bacterial contamination of the antacid suspension. The samples in this study were generic antacid suspensions with variations in storage temperature stored at 15-30°C (room), 2-8°C (cold), and 30-40°C (warm). Physical evaluations were carried out in the form of organoleptic tests, pH tests, viscosity tests and there was a bacterial contamination test. The results of the organoleptic test showed that the generic antacid suspension before and after storage had no change in smell, color and taste. The pH test has a stable pH and meets the requirements, namely pH between 7.3 and 8.5, the statistical test results obtained were 0.589> 0.05, which means that the pH before and after storage there was no significant difference. The viscosity test met the requirements, namely viscosity from 0.37 to 3.9 dpas. statistical test results obtained 0.023 <0.05, which means that the viscosity before and after storage there was a significant difference. The results of the Total Plate Number test before storage there was no contamination with ALTB values, namely 1.3x101 colonies/ml, 1,3x101 colonies/ml, and 1x101 colonies/ml, based on The International Pharmacopeia-7th edition the requirement for suspension bacterial contamination ≤ 1x102 colonies/ml, the result showed that contamination happened in all samples after storage. It can be concluded that bacterial contaminatios occured in all samples with temperature variations before and after storage but not affected on physical stability of antacid suspension.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.11904
      Issue No: Vol. 9, No. 2 (2022)
  • Efektivitas Larva Black Soldier Fly (Maggot) dalam Pengomposan Sampah
           Dapur dan Pemanfaatannya sebagai Buku Petunjuk Praktikum IPA SMP

    • Authors: Ika Lia Novenda, Rohmah Jwita Sari, Erlia Narulita
      Pages: 86 - 92
      Abstract: The problem of waste is increasingly complex, the composition of the largest contributor to waste is organic waste by 60%. Efforts to reduce waste are one of them by composting. Composting organic waste in a natural way takes 3-4 months, thus composting needs to use an effective decomposer to speed up composting, one of which is with the help of Black Soldier Fly (BSF). In addition, knowledge of composting needs to be applied at the education level. This study aims to determine the level of effectiveness of Black Soldier Fly larvae in composting waste and to determine the feasibility of a practical manual compiled from the results of research on the effectiveness of Black Soldier Fly (BSF) larvae. The research method uses experiments. The type of media used was vegetable, fruit and mixed waste, for the larvae used were 10 days old. The results showed that there were significant results, all media showed blackish brown color, humus smell, crumb texture, temperature 26-27°C, pH 6.8-7.1, and C/N ratio 2.75-6.81. %, as well as the junior high school science practicum instructions were categorized as very feasible to be used in helping the learning process with an average validity score of 88%.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.13377
      Issue No: Vol. 9, No. 2 (2022)
  • Kelayakan Modul Submateri Tumbuhan Paku Kelas X SMA dari Hasil
           Inventarisasi Pteridophyta Kabupaten Sanggau

    • Authors: Patrisia Vika, Syamswisna Syamswisna, Hayatul Fajri
      Pages: 93 - 101
      Abstract: Pteridophyta is one of difficult submaterial to understand by students during the learning process. It’s caused by the limitation of  supporting facilities such as media or teaching materials. Module could make students learning independently with or without guidance of teacher. This study aims to determine the feasibility of the module in the sub-material of ferns for class X SMA. The module of  the fern sub-material has been made based on the results of the Pteridophyta inventory in the Gunung Belungai Protected Forest, Sanggau Regency, in March-April 2021. The validity of the module teaching materials is calculated based on the Lawshe method. The validation of the fern module teaching materials was carried out by 5 validators consisting of 2 Biology Education lecturers and 3 Biology subject teachers. Based on the results of data analysis from the five validators, the validity  for each criteria are 3 (good) and 4 (very good) so the module of teaching materials get a CVR 0.99 and CVI 1. Module of submaterial of fern are valid and feasible to used as learning media.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.13787
      Issue No: Vol. 9, No. 2 (2022)
  • Fraksinasi Ekstrak Metanol Buah Parijoto (Medinilla speciosa Blume) dengan
           Pelarut Metanol, Etil Asetat, dan N-Heksana

    • Authors: Nailil Mona, Dyah Ayu Widyastuti, Atip Nurwahyunani, Fafa Nurdyansyah
      Pages: 102 - 109
      Abstract: Parijoto fruit (Medinilla speciosa Blume) is an endemic fruit around Mount Muria, Kudus, Central Java. The native people utilize Parijoto as traditional herbal medicine. Those utilization due to its metabolite compounds, such as tannin, flavonoid, fenolic, terpenoid, etc. In order to utilize Parijoto fruit properly, it is necessary to have an appropriate extraction and fractination method. So, the bioactive compounds of Parijoto fruit can be optimally extracted. The objective of this study is to obtain fractions of Parijoto fruit using three different solvent, methanol, ethyl acetate, and n-hexane. This study perform maceration as extraction method and liquid-liquid fractination to obtain the fractions. The dry simplicia yield was 85.49%. The maceration result was in the form of a blackish red green liquid with 67.45% yield. The methanol fraction was a thick blackish red liquid with 45.4% yield. While, the ethyl acetate fraction was a slightly fibrous viscous liquid with pinkish milk brown colour and 22.8% yield. On the other hand, the n-hexane fraction was a greenish viscous liquid with 13.8% yield. The highest yield is the result of fractination with methanol as a solvent because it is the most polar among others in this study. The solvent polarity is greatly affects the yield of fractination from natural product materials. In this study, the order of Parijoto yield from the highest to the lowest is methanol fraction, ethyl acetate fraction, and the last is n-hexane fraction. Those results were obtained due to the differences of each solvent polarity.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.13883
      Issue No: Vol. 9, No. 2 (2022)
  • Aktivitas Anticandida Herba Krokot (Portulaca grandiflora)

    • Authors: Agus Purwanto, Ch. Endang Purwaningsih, Christiana Indriasari
      Pages: 110 - 117
      Abstract: The purslane plant has been designated by the World Health Organization as one of the most widely used medicinal plants in the world and is known as "Global Panacea". The diversity of purslane plants from the genus Portulaca amounts to about 40-100 species found in the tropics and in areas that have four seasons. Candidiasis is one of the most common fungal infections in humans. Candidiasis is an opportunistic infection caused by excessive growth of the genus Candida fungus, 70% of Candida infections are caused by Candida albicans. Research on the antifungal activity of the purslane plant P. grandiflora is still rarely done. This study aims to determine the effective dose of anticandidal activity of purslane herb (P. grandiflora) against the C. albicans test microbe. This research is an experimental study that includes in vitro biological testing with the disc diffusion method, namely testing the inhibition of the extract of P. grandiflora 20%, 40%, 60%, 80%, and 100% against the growth of the fungus C. albicans. The antifungal activity test was carried out by observing the diameter of the inhibition zone formed around the paper disk. Anti-candida activity test of purslane herb (P. grandiflora) magenta variety extract showed that there was a zone of inhibition that was significantly different between all treatment groups. The measurement of the inhibition zone in the treatment group with a concentration of 20 to 100%, respectively, was 6.33±20; 6.86±13; 7.59±21; 8.33±33; 9.66±21; 25.69±98, thus indicating a moderate inhibition category for the growth of C. albicans.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14234
      Issue No: Vol. 9, No. 2 (2022)
           DAN Aspergillus niger

    • Authors: Amaliyah Nurul Hidayah, Ayuk Lestari, Mikhania Christiningtyas Eryani, Siti Nur Azizah
      Pages: 118 - 126
      Abstract: The high cost of instant culture media on the market has become the background of research to develop Mas banana (Musa acuminata Colla) Agar as a culture medium for Staphylococcus aureus and Aspergillus niger. Culture media is a mixture of nutrients or food substances needed by microorganisms for their growth. The purpose of this study was to determine the potential of Mas banana Agar (Musa acuminata Colla) at various concentrations of 2%, 4%, 6%, and 8% on the number of Staphylococcus aureus colony and Aspergillus niger micellium diameter. The type of research used was experimental research. The research stages were making Mas banana flour, prepared suspension of Staphylococcus aureus and Aspergillus niger, prepared Mas banana Agar, bacterial culture (spread method) incubated for 24hours at 37°C and mold (dot method) were incubated for 7 days at 37°C. Based on the results, S. aureus colonies on Mas banana Agar at 2%, 4%, 6% and 8% were 43.67 colonies, 52 colonies, 17.33 colonies and 7.67 colonies respectively. The average of Aspergillus niger mycelium diameter at 2%, 4%, 6% and 8% were 11.43 mm, 12.63 mm, 25.26 mm and 62.33 mm respectively. It can be concluded that Staphylococcus aureus and Aspergillus niger cultures growth on Mas banana Agar (Musa acuminata Colla) at various concentrations of 2%, 4%, 6%, 8%.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14237
      Issue No: Vol. 9, No. 2 (2022)
  • Kajian Farmakoinformatika Senyawa Alkaloid Anggur Laut (Caulerpa racemose)
           Sebagai Inhibitor Collagenase Dalam Mekanisme Antiaging

    • Authors: Dewi Ratih Tirto Sari, M.Eko Pranoto, Siti Zamilatul Azkiyah
      Pages: 127 - 133
      Abstract: Caulerpa racemosa or sea grape is an edible macroalgae, cultivated and wild species in Asia ocean. Sea grapes also well known as functional food source from marine, contains high protein, fibers and secondari metabolites. The biological functions  of sea grape also has been identified as antioxidant, antiinflammatory, antiobesity, and anticancer. However, the antiaging activity has not been known yet. This study investigated the potential antiaging activity of alkaloids compounds from sea grapes through inhibiting collagenase protein by farmacoinformatic study. six alkaloid structure of sea grapes including Caulerpin, Caulersin, Caulerchlorin, Racemosin A, Racemosin B, and Racemosin C, were retrieved from PubChem database and collagenase protein also was downloaded from Protein Data Bank with ID 2TCL. Six alkaloids of Caulerpa racemosa were redocked with collagenase at the specific site and were visualized by Discovery studio version 21.1.1. collagenase inhibitor compound also used as control for this study. farmacoinformatic analysis performed that six alkaloid of sea grapes and inhibitor compounds were showed at the same region of collagenase, which was identified as inhibitor sites. Several active residues of collagenase, involved SER139, PRO138, HIS128, and LEU81 were detected at six alkaloids compounds and control inhibitor, indicating six alkaloid of sea grapes potentially as collagenase inhibitor leading to antiaging properties. This study summarized that the six sea grape’s alkaloid has potential as collagenase inhibitor. Further in vivo analysis were required for further investigation.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14434
      Issue No: Vol. 9, No. 2 (2022)
  • Kesadaran Metakognitif Mahasiswa Melalui Pembelajaran Mandiri

    • Authors: Wachidatul Linda Yuhanna, Nuri Ati Ningsih
      Pages: 134 - 141
      Abstract: Metacognitive awareness is essential in learning, which includes knowledge about cognition and regulation about cognition. This study aims to 1) To know the average value of metacognitive awareness of biology education students which includes knowledge about cognition and regulation about cognition. 2) To know the percentage of student knowledge about cognition. 3) To know the percentage of student regulation about cognition. 4) To know the percentage of students metacognitive awareness. The instrument used is Metacognitive Awareness Inventory (MAI) developed by Schraw and Dennison (1994) which contains 52 questions with a score of 1 (true) and 0 (false). The results show that the mean value of metacognitive awareness is declarative knowledge (5,4); procedural knowledge (2,6); conditional knowledge (3.8); planning (4.9); information management strategy (6.4); understanding monitoring (4.8), search strategy (4.3) and evaluation (3.6). The percentage of knowledge about cognition of students with high (37.78%); medium (15.50%) and low (46.67%). The percentage of regulation about cognition of students with high (34.44%); medium (34.44%) and low (31.12%). The percentage of students metacognitive awareness with high (36.67%), medium (28.89%) and low (34.44%) so that there should be efforts to increase metacognitive awareness in biology education students.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14435
      Issue No: Vol. 9, No. 2 (2022)
  • Keanekaragaman Jenis Tanaman Biofarmaka di Objek Wisata Kesehatan Jamu
           Kalibakung Tegal sebagai Implementasi E-book Pada Pembelajaran Biologi

    • Authors: Salsabila Zahirotul Maghfiroh, Fibria Kaswinarni, Rivvana Citraning Rahayu
      Pages: 142 - 152
      Abstract: The diversity of Biopharmaca plant species in the Kalibakung Jamu Health Tourism area has not been fully utilized by the surrounding community, the community is only familiar with a few plants which makes this one of the factors not utilizing plants in Kalibakung Jamu Health Tourism. The purpose of this study was to determine the diversity of Biopharmaca plant species in Kalibakung Jamu Health Tourism. The research method used is quantitative and qualitative methods. Observations were made using the plot method with each plot measuring 15x15 meters for tree vegetation, 10x10 meters for shrub vegetation and 2x2 meters for herbaceous vegetation. Data were analyzed using the Shannon Wiener Diversity Index (H') and the potential of Biopharmaceutical plants was analyzed qualitatively. From the results of the study it was known that the types of vegetation found in the area were trees, shrubs and shrubs with an H level for tree vegetation of 1.530, shrub vegetation 0.625 and shrub vegetation 1.745.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14436
      Issue No: Vol. 9, No. 2 (2022)
  • Kajian Theileiriosis pada Sapi Di Kabupaten Pohuwato

    • Authors: Tri Ananda Erwin Nugroho, Muhammad Sayuti, Elvin Amuda
      Pages: 153 - 157
      Abstract: Blood parasites such as Babesia, Theileria, Trypanosoma and Anaplasma are disease agents that have a predilection for cattle blood which can cause losses in the cattle breeding sector. This study aims to conduct a study of Theileiriosis in cattle in Pohuwato District. Sampling of cattle blood was carried out proportionally in 13 sub-districts in Pohuwato Regency with a total of 100 samples from a total population of 29,266 cattle. Blood samples were taken through the jugular vein. Examination of the samples used the thin blood smear method with Giemsa staining and observed with a 1000 magnification microscope. The incidence rate of Theileiriosis was determined by counting the number of positive blood samples infected with Theileria sp., divided by the total number of samples examined multiplied by one hundred percent and then the data were analyzed descriptively. Based on the results of the study, of the 100 blood samples examined, 31 blood samples were found Theileria sp., in the observed blood smear preparations. The incidence rate of Theileiriosis in cattle in Pohuwato District is 31%.
      PubDate: 2022-11-25
      DOI: 10.25273/florea.v9i2.14437
      Issue No: Vol. 9, No. 2 (2022)
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