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Expert Opinion on Astronomy and Astrophysics     Open Access   (Followers: 2)
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Journal of Medicines Development Sciences
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  This is an Open Access Journal Open Access journal
ISSN (Print) 2382-6363 - ISSN (Online) 2382-6371
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  • Determination of Genistin Content in Flemingia philippinensis Merr. et
           Rolfe by High Performance Liquid Chromatography (HPLC)

    • Authors: Xiaoqing Wang, Huiying Zhang
      Abstract: Objective: To set up the HPLC method in determination of the genistin content in Flemingia philippinensis Merr. et Rolfe. Method: Extract and filter the crushed Flemingia philippinensis Merr. et Rolfe that has been soaked in methanol, the filtrate of which is to be taken out as the sample. Shimadzu LC-2010A is adopted in this experiment which is performed on Agilent Extend-RP column with acetonitrile-0.1% phosphoric acid aqueous solution (19:81) as mobile phase. The flow rate is 1.0 mL/min and the UV detective wavelength is 262 nm. Result: The linear relationship is excellent with higher recovery rate within the range. This method is rapid, simple and accurate.
      PubDate: 2018-10-31
      DOI: 10.18063/jmds.v3i1.897
      Issue No: Vol. 3, No. 1 (2018)
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Heriot-Watt University
Edinburgh, EH14 4AS, UK
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