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Immunohematology : Journal of Blood Group Serology and Molecular Genetics
Number of Followers: 1  Hybrid journal (It can contain Open Access articles)ISSN (Print) 0894-203X - ISSN (Online) 1930-3955 Published by Exeley Inc  [23 journals]
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- Anti-Jk3 in a Filipino man
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Abstract: A 62-year-old Filipino man with a history of chronic obstructive pulmonary disease, hypertension, and hyperlipidemia was admitted to the emergency department at Hospital A with recurrent fevers, weakness, and jaundice. The patient was evaluated and eventually discharged with a diagnosis of possible drug-induced hepatitis. One month later, the patient was
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- CD59: A long-known complement inhibitor has advanced to a blood
group system-
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Abstract: The blood group system number 35 is based on CD59, a 20-kDa membrane glycoprotein present on a large number of different cells, including erythrocytes. The major function of CD59 is to protect cells from complement attack. CD59 binds to complement components C8 and C9 and prevents the polymerization of C9,
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- Red cell antigen prevalence predicted by molecular testing in ethnic
groups of South Texas blood donors-
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Abstract: Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were
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- Weak D type 67 in four related Canadian blood donors
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Abstract: Correct donor D typing is critical to prevent recipient alloimmunization. No method can detect all variants, and the immunogenicity of many variants is unknown. Routine ABO and D serologic typings are performed in our laboratory by automated microplate testing. Until 2011, routine confirmation of D– status of first-time donors was
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- Transfusion-related acute lung injury in an era of TRALI risk
mitigation-
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Abstract: Transfusion-related acute lung injury (TRALI) is a rare complication of transfusion, for which the true incidence remains obscure, since there are a number of factors that may lead to misdiagnosis. Despite this, it continues to be the leading cause of transfusion-associated mortality. Here we present a historical case of TRALI
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- Alternative to providing ABO-incompatible donors for patients in
end-stage renal disease: renal transplant registries, the need of
the hour-
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- Erratum
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- Blood group genotyping: the power and limitations of the Hemo ID Panel and
MassARRAY platform-
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Abstract: Matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS), is a sensitive analytical method capable of resolving DNA fragments varying in mass by a single nucleotide. MALDI-TOF MS is applicable to blood group genotyping, as the majority of blood group antigens are encoded by single nucleotide polymorphisms. Blood group genotyping by
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- Clinical and reference lab characteristics of patients with suspected
direct antiglobulin test (DAT)-negative immune hemolytic anemia-
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Abstract: Clinical evidence of warm autoimmune hemolytic anemia is present in 1 percent to 10 percent of patients whose direct antiglobulin test (DAT) is negative. The clinical underpinnings associated with DAT-negative immune hemolysis are poorly understood, and the current study aimed to further define the clinical characteristics associated with this form
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- Severe hemolytic disease of the fetus and newborn due to anti-C+G
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Abstract: Anti-G is commonly present with anti-D and/or anti-C and can confuse serological investigations. In general, anti-G is not considered a likely cause of severe hemolytic disease of the fetus and newborn (HDFN), but it is important to differentiate it from anti-D in women who should be administered anti-D immunoglobulin prophylaxis.
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- Blocked D phenomenon and relevance of maternal serologic testing
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Abstract: A blood requisition for double-volume exchange transfusion was received for a 2-day-old male child born to a 29-yearold multiparous female (P2002) referred to our institute having neonatal jaundice with encephalopathy; no maternal sample was received. The neonatal blood sample was typed as group A, D–, and the direct antiglobulin test
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- HEA BeadChip™ technology in immunohematology
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Abstract: Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their presence or absence on the red cell membrane. Several molecular techniques have been developed
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- An overview of the use of SNaPshot for predicting blood group antigens
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Abstract: The use of SNaPshot (Applied Biosystems, Foster City, CA) for predicting blood group antigens has emerged as an alternative to hemagglutination testing and also to the current low- and highthroughput blood group genotyping methods. Several groups have developed multiplex–polymerase chain reaction SNaPshot assays to determine single nucleotide polymorphisms (SNPs) in
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- An overview of the Progenika ID CORE XT: an automated genotyping platform
based on a fluidic microarray system-
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Abstract: Automated testing platforms facilitate the introduction of red cell genotyping of patients and blood donors. Fluidic microarray systems, such as Luminex XMAP (Austin, TX), are used in many clinical applications, including HLA and HPA typing. The Progenika ID CORE XT (Progenika Biopharma-Grifols, Bizkaia, Spain) uses this platform to analyze 29
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- CD177/NB1 receptor expression is dynamically regulated in sepsis
patients-
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- Multiplex ligation-dependent probe amplification assay for blood group
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Abstract: The blood group multiplex ligation-dependent probe amplification (MLPA) is a comprehensive assay, developed for genotyping the majority of clinically relevant blood group antigens in both patients and donors. The MLPA is an easy method to apply and only requires a thermal cycler and capillary electrophoresis equipment. Because the molecular basis
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- Comparative evaluation of gel column agglutination and erythrocyte
magnetized technology for red blood cell alloantibody titration-
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Abstract: Antibody titration is traditionally performed using a conventional test tube (CTT) method, which is subjected to interlaboratory variations because of a lack of standardization and reproducibility. The aim of this study is to compare newer methods such as gel column technology (GCT) and erythrocyte magnetized technology (EMT) for antibody titration
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- High-resolution melting analysis as an alternative method for human
neutrophil antigen genotyping-
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Abstract: Human neutrophil antigen (HNA)-typed granulocyte panels are widely used to screen for the presence of HNA antibodies and to determine antibody specificity. Many laboratories screen donors for HNA genotypes using low-throughput methods such as allele-specific polymerase chain reaction (PCR), PCR–restriction fragment–length polymorphism, and multiplex PCR. In the present study, we
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- Proposed criterion for distinguishing ABO mosaics from ABO chimeras using
flow cytometric analysis-
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Abstract: Differentiation of ABO mosaics from chimeras is performed using flow cytometry (FCM) analysis. Although mosaics and chimeras have been distinguished by presence or absence of clear resolution using FCM analysis, the lack of quantitative metrics and definitive criteria for this differentiation has made some cases difficult to differentiate. In this
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- Kidd blood group system: a review
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Abstract: The Kidd blood group system has been recognized as clinically important in red blood cell (RBC) serology since its identification in 1951. Forty years later, the JK glycoprotein was determined to be a product of SCL14A1 and was identical to the urea transport protein UT-B produced by HUT11A. The functional
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- Mass-scale donor red cell genotyping using real-time array technology
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Abstract: Blood centers are in the unique position to evaluate large numbers of blood donations for antigen-negative blood types. The limitations with the use of hemagglutination, however, can be circumvented with red cell genotyping. The reagents used for genotyping are synthesized and can be designed for any of the known blood
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- Kell and Kx blood group systems
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Abstract: The Kell and Kx blood group systems are expressed as covalently linked molecules on red blood cells (RBCs). The Kell blood group system is very polymorphic, with 35 antigens assigned to the system. The expression of Kell glycoprotein on RBCs is not critical to the erythrocyte function. However, the expression
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- A simple approach to screen rare donors in Brazil
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Abstract: Providing blood units for patients with an antibody to a highprevalence antigen or with multiple common antibodies is a constant challenge to the blood banks. Finding a compatible donor requires extensive screening, which incurs a large amount of investment. In this article, we share our experience of organizing a rare
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