Publisher: Universitas Udayana (Total: 62 journals)   [Sort by number of followers]

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Advances in Tropical Biodiversity and Environmental Sciences     Open Access   (Followers: 4)
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Buletin Studi Ekonomi     Open Access   (Followers: 2)
Buletin Veteriner Udayana     Open Access   (Followers: 1)
Bumi Lestari J. of Environment     Open Access  
Cakra Kimia (Indonesian E-J. of Applied Chemistry)     Open Access  
COPING (Community of Publishing in Nursing)     Open Access   (Followers: 1)
E-J. of Cultural Studies     Open Access   (Followers: 1)
e-J. of Linguistics     Open Access   (Followers: 3)
E-J. of Tourism     Open Access   (Followers: 8)
E-Jurnal Agroekoteknologi Tropika (J. of Tropical Agroecotechnology)     Open Access  
E-Jurnal Akuntansi     Open Access  
E-Jurnal Ekonomi dan Bisnis Universitas Udayana     Open Access  
E-Jurnal Ekonomi Pembangunan Universitas Udayana     Open Access  
E-Jurnal Manajemen Universitas Udayana     Open Access  
E-Jurnal Medika Udayana     Open Access  
Ecotrophic : J. of Environmental Science     Open Access  
Indonesia Medicus Veterinus     Open Access  
Indonesian J. of Legal and Forensic Sciences     Open Access   (Followers: 1)
Intisari Sains Medis     Open Access  
Intl. J. of Engineering and Emerging Technology     Open Access  
itepa : Jurnal Ilmu dan Teknologi Pangan     Open Access  
J. of Food Security and Agriculture     Open Access   (Followers: 1)
J. of Health Sciences and Medicine     Open Access  
J. of Marine and Aquatic Sciences     Open Access   (Followers: 2)
J. of Veterinary and Animal Sciences     Open Access  
JBN (Jurnal Bedah Nasional)     Open Access  
Jurnal Analisis Pariwisata     Open Access  
Jurnal Arsitektur Lansekap     Open Access  
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Jurnal Biologi Udayana     Open Access  
Jurnal Destinasi Pariwisata     Open Access  
Jurnal Ekonomi Kuantitatif Terapan     Open Access  
Jurnal Energi Dan Manufaktur     Open Access  
Jurnal Ilmiah Akuntansi dan Bisnis     Open Access  
Jurnal Ilmiah Mahasiswa SPEKTRUM     Open Access  
Jurnal Ilmiah Merpati (Menara Penelitian Akademika Teknologi Informasi)     Open Access  
Jurnal Ilmu dan Kesehatan Hewan (Veterinary Science and Medicine J.)     Open Access   (Followers: 1)
Jurnal Ilmu Komputer     Open Access  
Jurnal IPTA     Open Access  
Jurnal Kepariwisataan dan Hospitalitas     Open Access  
Jurnal Kimia (J. of Chemistry)     Open Access  
Jurnal Magister Hukum Udayana (Udayana Master Law J.)     Open Access  
Jurnal Master Pariwisata (J. Master in Tourism Studies)     Open Access  
Jurnal Matematika     Open Access  
Jurnal Rekayasa dan Manajemen Agroindustri     Open Access  
Jurnal Spektran     Open Access  
Jurnal Udayana Mengabdi     Open Access  
Jurnal Veteriner     Open Access   (Followers: 1)
Kertha Patrika     Open Access  
Lingual : J. of Language and Culture     Open Access  
Linguistika : Buletin Ilmiah Program Magister Linguistik Universitas Udayana     Open Access   (Followers: 1)
Lontar Komputer : Jurnal Ilmiah Teknologi Informasi     Open Access  
Majalah Ilmiah Peternakan     Open Access   (Followers: 1)
Majalah Ilmiah Teknologi Elektro : J. of Electrical Technology     Open Access   (Followers: 1)
Matrik : Jurnal Manajemen, Strategi Bisnis dan Kewirausahaan     Open Access  
Piramida     Open Access  
Ruang-Space: Jurnal Lingkungan Binaan (J. of The Built Environment)     Open Access  
Simbiosis : J. of Biological Sciences     Open Access  
Sport and Fitness J.     Open Access   (Followers: 4)
Udayana J. of Law and Culture     Open Access  
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Buletin Veteriner Udayana
Number of Followers: 1  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2085-2495 - ISSN (Online) 2477-2712
Published by Universitas Udayana Homepage  [62 journals]
  • Cover Vol. 14 No. 3 June 2022

    • Authors: Admin Buletinvet
      PubDate: 2022-05-17
       
  • LEVELS OF HORMON ESTROGEN IN BALI CATTLE DURING PUBERTY

    • Authors: Herdi Wahyu Adi Prananda, Desak Nyoman Dewi Indira Laksmi, I Gusti Ngurah Bagus Trilaksana
      Pages: 197 - 201
      Abstract: Bali cattle is one of the most important native cattle in Indonesia. There is one measure of reproductive efficiency. The achievement of early age of puberty in accordance with its genetic potential is important to achieve optimum reproductive performance and provide increase in productivity. One of the things that affects the appearance of puberty is the amount of estrogen in the blood serum. The purpose of this study was to determine the levels of estrogen in cattle blood serum. The study was conducted by recording the age first and then taking blood in cattle that had signs of puberty and then carried out checking hormone levels using the ELISA method. The results showed that the average age of puberty was 21.44 months and the average estrogen level at puberty was 84.75 ng / ml. Testing with correlation and regression tests showed a close relationship between age and estrogen levels at puberty with a correlation coefficient of r = 0.798.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p01
       
  • SENSITIVITY TEST OF STREPTOCOCCUS SPP. ISOLATED FROM PORCINE RESPIRATORY
           DISEASE COMPLEX AGAINST KANAMYCIN, STREPTOMYCIN AND DOXYCYCLINE

    • Authors: Dwi Arso Purba, Ketut Tono Pasek Gelgel, I Gusti Ketut Suarjana
      Pages: 202 - 209
      Abstract: Respiratory diseases in pigs often occur in the production of modern pigs around the world and known as Porcine Respiratory Disease Complex (PRDC). Prevention of PRDC can only be done if the infecting agent is bacteria. The bacteria that are most often isolated in complex pig duct disease are Streptococcus spp. This study was conducted to determine the sensitivity pattern of Streptococcus spp. isolated from animals with PRDC symptoms on kanamycin, streptomycin and doxycycline. Isolates were taken from the results of the PRDC case study of pig origin in Tabanan, Gianyar and Badung regencies. Pure isolates were then planted in blood agar and a sensitivity test was carried out using the Kirby-Bauer method for each antibiotic. Bacteria can be resistant, intermediate and sensitive to antibiotics tested. This gives rise to a pattern of sensitivity to antibiotics so that resistant germs will cause treatment failure. The results showed that hemolytic ' Streptococcus isolates were 60% sensitive to kanamycin, 100% sensitive to streptomycin, 40% resistant to kanamycin and 100% resistant to doxycycline. While hemolytic ' Streptococcus isolates were 100% sensitive to kanamycin and streptomycin but 100% were resistant to doxycycline.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p02
       
  • CLUSTERIZATION OF BALI CATTLE FEED MANAGEMENT AT SIMANTRI IN BADUNG
           REGENCY

    • Authors: Geidha Lailia Luzain, I Putu Sampurna, Tjok Sari Nindhia
      Pages: 210 - 216
      Abstract: This study aims to determine the components of feed management that have not been applied intensively in Simantri at Badung Regency and find out that Simantrihas applied feed management intensively. Sampling was done by saturated sampling technique at 50 Simantri. The data obtained were analyzed using hierarchical clusters with dendogram cluster plots with intensive, semi-intensive, and extensive characterizing variables. The results showed feed management components that have not been applied intensively include feeding frequency, feeding time, type of feed given, amount of feed given, and the presence or absence of remaining food. Simantri in Badung Regency which has not implemented intensive feed management as many as 40 Simantri and most of the feed management components have not been applied intensively. The conclusion of this study is that not all Simantri in Badung Regency have applied all components of feed management intensively, and most of Simantri in Badung Regency applied semi-intensive feed management. Suggestions need to be optimized through counseling to achieve a maximum Simantri program.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p03
       
  • THE EFFECT OF LAYING AT ROOM TEMPERATURE ON THE PH VALUE AND TOTAL
           BACTERIA OF BALI BEEF

    • Authors: Ni Putu Tessa Arsaning Rahayu, Kadek Karang Agustina, Ida Bagus Ngurah Swacita
      Pages: 217 - 224
      Abstract: Bali beef is an ideal medium for the growth of microorganisms, because it is rich in protein, minerals and water. When placed at room temperature, the meat will spoil more quickly. The purpose of this study was to determine the effect of laying beef at room temperature on the pH value and total bacteria. This research method tested 24 pieces of beef with a weight of 10 grams each and placed at room temperature (27-30°C) then tested with pH and total bacteria tests. The research design used was Completely Randomized Design (CRD) with 4 treatments of laying time at room temperature for 0 hours, 3 hours, 6 hours, and 9 hours with 6 replications. The research data were analyzed by means of variance and if the results were significantly different, it was continued with Duncan's test. The results showed that laying beef at room temperature had no effect on the pH value (P>0.05), but had a very significant effect (P<0.01) on increasing total bacteria. It concluded that the quality of bali beef placed at room temperature will reduce the quality
      PubDate: 2022-01-30
      DOI: 10.24843/bulvet.2022.v14.i03.p04
       
  • PREVALENCE OF TRICHURIS SPP. INFECTION IN BALI CATTLE IN DUKUH SARI
           FARMING GROUP, PEMPATAN VILLAGE, RENDANG SUB-DISTRICT, KARANGASEM REGENCY
           OF BALI

    • Authors: Rio Fadly Sihombing, Ida Bagus Made Oka, Anak Agung Gde Arjana
      Pages: 225 - 230
      Abstract: This study aims to determine the prevalence of Trichuris spp. worm infection in Bali cattle and whether the relation of sex, age, and number of cows in the cage to the prevalence of Trichuris spp. worm infection in the Dukuh Sari cattle group, Pempatan village, Rendang sub-district, Karangasem district. The samples used were 102 Bali faeces and examined using floating concentration method, identification of worm eggs based on morphology and morphometry. The results of the examination found 6 samples (5.9%) positively infected with worms Trichuris spp. After statistical analysis through chi-square test, differences in age, sex, and number of cows in the cage did not affect the infection of Trichuris spp.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p05
       
  • PERCENTAGE OF BASOPHILS, EOSINOPHILS, AND NEUTROPHILS IN BALI CATTLE BLOOD
           BASED ON ORGANIC FEED

    • Authors: Putu Ayu Santika, Ni Ketut Suwiti, Ni Luh Eka Setiasih
      Pages: 231 - 237
      Abstract: One of the Bali cattle breeding maintenance is organic-based feeding. Organic-based feed is likely to not be as optimal as the composition of feed like feed in general, such as minerals and other organic substances. The condition and composition of the feed can affect the number of blood leukocytes and cause changes in the percentage of leukocyte cells such as basophils, eosinophils and neutrophils. This study aims to determine the percentage of basophils, eosinophils, neutrophils in bali cattle blood based on organic feed. This study was carried out on a farm owned by residents of the Village of Bulian, Kubutambah District, Buleleng Regency. Samples in the form of blood taken from 10 female bali cattle by vein using venoject. Next, blood smear preparations were made and then fixed and stained by Harris Hematoxillin-Eosin (HE). Percentage of basophils, eosinophils, neutrophils calculated by cross-sectional method. Data obtained and analyzed with descriptive quantitative. The results showed that cattle raised with an organic based maintenance system experienced basophilia, eosinophilia, and neutropenia.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p06
       
  • PREVALENCE AND INTENSITY INFECTION OF STRONGYLOIDES RANSOMI WORMS IN PIGS
           SLAUGHTERED AT PESANGGARAN SLAUGHTER HOUSE DENPASAR CITY

    • Authors: Muhammad Wilmar Akbar, Nyoman Adi Suratma, Ida Bagus Made Oka
      Pages: 238 - 245
      Abstract: This study aims to determine the prevalence and intensity of Strongyloides ransomi infections in pigs that were slaughtered at Pesanggaran animal slaughter house in Denpasar city. This study used 200 samples of pig feces. Stool samples are examined to determine the prevalence of infection using the floating method while the intensity of the infection determined using the Mc. Master method. The data obtained were presented descriptively. The results showed that the prevalence of S. ransomi infections was 5% with the intensity of infection was found that less than 500 eggs per gram of feces, it were categorized as having only mild infections.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p07
       
  • HISTOPATHOLOGICAL DESCRIPTION OF THE LIVER OF WHITE RATS TREATED WITH
           YEAST

    • Authors: Ainul - Hidayah, Putu Suastika, Ketut Budiasa, Samsuri Samsuri, I Ketut Berata, Luh Made Sudimartini
      Pages: 246 - 254
      Abstract: This study aimed to determine the histopathological picture of the liver of white rats due to the treatment of yeast at several different doses. A total of 24 white rats were randomly divided into 4 treatment groups, namely P0: control; P1: giving yeast of 100 mg/kg BW; P2: 200 mg/kg BW; and P3: 300 mg/kg BW. The treatment dose was carried out for 21 days. On the 22nd day, all samples got euthanasia and a necropsy procedure was performed. The liver organs were taken to make histological preparations by staining with Hematoxylin Eosin. The variables observed included fatty degeneration and necrosis. The data obtained were analyzed using the Kruskal-Wallis test. The results showed that the administration of yeast at a dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW was not significantly different (P>0.05) compared to the control (P0). It can be concluded that threating by yeast up to a dose of 300 mg/kg BW does not cause histopathological changes in the liver of white rats.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p08
       
  • THE EFFECT OF HERB AND YEAST ADDITION ON THE ORGAN PROFILE AND DIGESTIVE
           TRACT OF BROILER CHICKENS

    • Authors: Ni Sri Yuliani, Gerson Y. I. Sakan, Ni Putu F. Suryatni
      Pages: 255 - 262
      Abstract: This study aimed to examine the effect of herbs and yeast addition to drinking water on the weight and length of the digestive tract of broiler chickens. The study design used a completely randomized design with four treatmens and five repetitions. Group P1: Herbal medicene 10 ml/Liter drinking water, P2: Herbal medicine 10 ml added with yeast 1 g/Liter drinking water, P3: Yeast 1g/Liter drinking water, PO: Controls given water. Data analysis used analysis of variance (Anova) and if the result had a significant effect, it was continued with Duncan's Multiple Distance tes. The addition of herbs and yeast to drinking water had an effect on the size of the spleen and pancreas and significantly (P<0,05) on the weight of the spleen and pancreas, but had no effect (P> 0,05) on the weight and length of the small intestine, liver and broiler final weight. The addition of herbs and yeast to drinking water can reduce the weight of the small intestine and shorten the intestine, increase the weight of the spleen and pancreas, but have the same effect on the final weight. The use of herbal medicene should be fermented together with yeast to determine its effectiveness in the digestive tract.
      PubDate: 2022-04-17
      DOI: 10.24843/bulvet.2022.v14.i03.p09
       
  • COMBINATION OF MORINGA LEAF FLOUR AND PROBIOTICS IN FEED ON THE GROWTH AND
           SURVIVAL RATE OF TILAPIA JUVENILE

    • Authors: Nanang Satria Sumarjan, Siti Hilyana, Fariq Azhar
      Pages: 263 - 273
      Abstract: This study aims were to supplement Moringa leaf meal and probiotics into feed. Moringa oliefera leaves are plants that contain high enough protein for growth and contain flavonoids, saponins, and vitamins A, B, C, and E which can trigger endurance, and probiotics can increase the digestibility of food in the fish intestines, so that absorption the nutrients in the feed can be completely absorbed. This study used a completely randomized design consisting of 5 treatments and 3 replications, in order to obtain 12 experimental units. in treatment A (4% moringa leaf meal + 6 ml / kg of feed), B (6% moringa leaf meal + 6 ml / kg of feed), C (8% moringa leaf meal + 6 ml / kg of feed), D (positive control) and E (negative control). Parameters measured were absolute weight growth, absolute length growth, specific weight growth, specific length growth, survival, water quality, and feed conversion. The results showed that the addition of Moringa leaf meal combined with probiotics in the feed had a significant effect on the absolute weight of 4.673 gr, absolute length 2.607 cm, specific weight 2.25%, specific length 1.07%, feed conversion 1.84%, but no significant effect on the survival rate. live tilapia seeds, with the optimum dose to provide the best growth in tilapia seeds is treatment B with a dose of 6% Moringa leaves and 6 ml / kg of probiotic feed.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p10
       
  • DETECTION OF BACTERIA WITH POTENTIAL PATHOGENS IN PORCINE RESPIRATORY
           DISEASE COMPLEX

    • Authors: I Gusti Ketut Suarjana, I Nengah Kerta Besung, Ketut Tono Pasek Gelgel, Putu Henrywaesa Sudipa
      Pages: 274 - 279
      Abstract: This study aims to detect bacterial infectious agents in pigs with complex respiratory tract disease known as porcine respiratory disease complex (PRDC) in some pig herds in Bali and look for the right medication is use to handle the incidence. The research sample comes from Tabanan, Badung and Gianyar totaling 48 Samples were isolated and identified by methods: isolation, the primary test, biochemical test. Test of sensitivity to antibiotics using disc diffusion method according to Kirby-Bauer method. The result showed that bacterial agents isolated from pigs were among others Streptococcus spp. alfa hemolytic non-coagulase (41,6%), Staphylococcus spp. beta hemolytic noncoagulase (41,6%) and Escherichia coli. Staphylococcus spp. beta hemolytic non-coagulase and E. coli were sensitive to streptomycin, kanamycin, doxicyclin, clindamycin and bacitracin. To conclude, Streptococcus spp. alfa hemolytic non-coagulase were sensitive to streptomycin, kanamycin, clindamycin and bacitracin, but resisten to doxicyclin.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p11
       
  • BLOOD RESULTS IN PIGS INFECTED WITH STREPTOCOCCUS SUIS INTRANASALLY AND
           INTRAVENOUSLY

    • Authors: Putri Wiliantari, I Nengah Kerta Besung, Gusti Ngurah Kade Mahardika
      Pages: 280 - 286
      Abstract: Research has been carried out to elucidate clinical, as well as differences in blood profiles in pigs infected with Streptococcus suis isolated from Bali by intravenous and intranasal infection routes. S.suis isolate of PPB5 were inoculated intranasally and intravenously to three pigs respectively. The pigs used in this study were 8 weeks old. In intranasal infection before inoculation, 1% acetic acid was first applied to the nasal cavity, then S. suis inoculum was sprayed (2 x 106). In intravenous infection, inoculum fluid is injected into the auricular vein. Clinical changes in pigs were observed until day 15. Blood samples were taken before being inoculated with bacteria, 3rd day after inoculation, and before being euthanized. On the 3rd day after inoculation, the number of eosinophils was significantly different in the two treatments, while the other blood profiles were not significantly different. All blood indicators in the two treatments were not significantly different before the animals were euthanized on day 15. The increase number of erythrocytes and monocytosis was found in intranasal infections at that day. Hypohaemoglobinemia and decreased PCV are seen in both intranasal and intravenous infections. Further research is needed with a greater number of replications. The S. suis vaccine needs to be developed immediately to reduce the threat to public health and economic losses for farmers.
      PubDate: 2022-01-30
      DOI: 10.24843/bulvet.2022.v14.i03.p12
       
  • STAPHYLOCOCCUS SPP. BACTERIA IN KOI FISH REARED DURING QUARANTINE PERIOD

    • Authors: Syafiana Fairizca, Hapsari Mahatmi, Putu Henrywaesa Sudipa
      Pages: 287 - 294
      Abstract: Currently, koi fish enthusiasts and fans in various regions in Indonesia, especially in Bali, have increased very sharply. In the conditions of transportation from outside Bali to Bali, it triggers stress which causes the koi's resistance to decrease so that disease occurs. Therefore, a quarantine process is very necessary to get koi that are healthy and suitable for cultivation. Diseases in koi fish can be caused by viruses, parasites, and bacteria, one of which is Staphylococcus spp. Research on bacterial infections in koi fish is still very rare, especially regarding Staphylococcus spp. in koi fish in Indonesia and in Bali. This study was conducted to obtain preliminary data regarding Staphylococcus sp infection in koi fish. In this study, the object used was koi fish which has any symptomps of diseases or died during a 14-day quarantine period.The researched koi fish came from suppliers in Tulungagung and Blitar, East Java. The size of the koi fish ranged from 15 -25 cm which was kept in fish isolation ponds at Raka koi and Tilapia Farm, Denpasar. The samples taken were 19 samples, the 8 samples are thescale swabs from koi fish from Tulungagung and 11 scale swab samples from koi fish from Blitar using random sampling technique. Staphylococcus sp was isolated with Blood Agar and MacConkey Agar media and identified by Gram stain test and catalase test. From the results of the isolation and identification of bacteria, 1 sample was positive for Staphylococcus spp. (5.26%).
      PubDate: 2022-01-30
      DOI: 10.24843/bulvet.2022.v14.i03.p13
       
  • THE ANGIOGENESIS ACTIVITIES OF GEL CACAO SEED EXTRACT ON GUM INCISION
           WOUND HEALING IN GUINEA PIGS

    • Authors: Ketut Novi Kusmayani, Anak Agung Gde Jaya Warditha, I Ketut Berata
      Pages: 295 - 301
      Abstract: One of alternative herbal therapy ingredients, cocoa beans because it contains flavonoids, polyphenols, and alkaloids can reduce number of macrophages and antioxidants, to accelerate inflammatory process. This study was aimed determine effect cocoa bean extract gel on healing guinea pig gum incisions based on level angiogenesis. This study was experimental study using completely randomized design, with four treatments with 6 replications, total of 24 experimental animals. Guinea pigs were injected with ketamine at dose of 50 mg/kg BW anesthetic, then incision made in gums with length 10 mm and depth 2 mm. Then given treatment of topical cocoa bean extract gel, (T0) group with concentration 0%, (T1) 16%, (T2) 24%, (T3) 32%, each for 5 days. On 6th day euthanasia was performed and gum tissue was taken. Gum tissue was processed preparation of histology preparations using Hematoxylin-Eosin (HE) staining method. Histopathological examination preparations were carried out with a microscope based on level angiogenesis. Results of histopathological examination obtained an average level angiogenesis at To=19.00, T1=38.00, T2=43.50, T3=47.83. Statistical analysis showed that there was a significant increase in angiogenesis between controls (T0) compared to group given cocoa bean extract gel (T1, T2 and T3). But between treatment groups T1, T2 and T3 there was no significant difference. It can conclude topical application of cocoa bean extract gel has been shown increase angiogenesis in guinea pig gum incision wound healing. Administration of cocoa bean extract with a concentration of 32% resulted in highest angiogenesis rate.
      PubDate: 2022-01-30
      DOI: 10.24843/bulvet.2022.v14.i03.p14
       
  • HISTOLOGICAL STUCTURE OF THE DOG’S SKIN WITH DERMATITIS IN EXTREMITAS
           CAUDAL, DORSUM, AND ABDOMEN

    • Authors: Putu Dian Purnama Putra, Ni Ketut Suwiti, Ni Nyoman Werdi Susari
      Pages: 302 - 309
      Abstract: Skin is the largest organ in dogs that serves to overcome the presence of foreign agents both from exogenous and endogenous. One of the health problems on dog skin is dermatitis and can be found in almost all parts of the body. This study aims to observe changes in the histological structure of the skin of male and female dogs suffering from dermatitis on the caudal, dorsum, and abdominal extremities. A total of 24 research samples in the form of skin organs were collected from patients at the Udayana University Animal Hospital Teaching. The results showed that there was no difference (P>0.05), the histological structure of the skin of male and female dogs suffering from dermatitis, both skin from the caudal extremities, dorsum and abdomen. Histological changes found were: parasitic segments, hiperkeratosis, necrosis, degeneration, edema, hyperplasia, infiltration of stratum reticular fat cells, and infiltration of inflammatory cells. Infiltration of inflammatory cells, neutrofils, and macrophages are mostly found in the dermis while basofils are found in the epidermis.
      PubDate: 2022-01-30
      DOI: 10.24843/bulvet.2022.v14.i03.p15
       
  • ANALYSIS OF PATHOGENIC GENE MARKER hlyF IN ESCHERICHIA COLI CAUSES OF
           COLLIBACILLOSIS IN FREE-RANGE CHICKEN

    • Authors: I Gede Eka Chandrawan, Gusti Ngurah Kade Mahardika, I Nengah Kerta Besung, I Gusti Ketut Suarjana
      Pages: 310 - 318
      Abstract: Colibacillosis by Avian Pathogenic Escherchia coli (APEC) is an infectious disease in poultry. The ability of APEC to cause disease depends on various virulence factors, including hlyF. The study aimed to prove the existence of the APEC hlyF gene in Bali and to find out the DNA sequences of these hlyF genes and their kinship with the hlyF gene from other countries. This study used two APEC isolates from free range chicken in Tabanan and Badung regencies. The isolates have been purified and were available at the Bacteriology Laboratory of Veterinary Medicine Faculty, Udayana University. DNA isolates were isolated using Chelex 10%. The hlyF gene is amplified using published DNA primers by polymerase chain reaction (PCR). PCR products were sequenced at First Base Laboratories in Malaysia using the Sanger’s Dideoxy Nucleotide Termination method. Both the hlyF gene sequences that can be analyzed have 100% homology with a readable length of 518 bp. Phylogenic test with 25 DNA sequences of hlyF gene on Escherichia coli and other bacteria in the world using UPGMA method with bootstrap (500 repetitions) was conducted with MEGA 5.2. All data have six polymorphic sites of nucleic acid and two polymorphic sites of amino acid . The hlyF gene from Bali was in same group with hlyF genes from various countries in the world. This gene can be used as a pathogenic marker of APEC in Indonesia.
      PubDate: 2022-01-29
      DOI: 10.24843/bulvet.2022.v14.i03.p16
       
 
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