| Publisher: Mazandaran University of Medical Sciences
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Research in Molecular Medicine
Number of Followers: 0  Subscription journalISSN (Print) 2322-1348 - ISSN (Online) 2322-133X Published by Mazandaran University of Medical Sciences  [1 journal]
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- Molecular Identification of Trichostrongylus Species Among Small Ruminants
in Mazandaran Province, Iran-
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Authors: Shirzad Gholami
Abstract:
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- Influence of Bilirubin, Hemin, Zinc Protoporphyrin, Glutathione, Curcumin,
and Their Combinations as a Supplement to Support the Viability and
Functionality of Pancreas and Liver Cells-
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Authors: Durdi Qujeq
Abstract: Background: Previous experiments have shown different responses of pancreas and liver cells to the culture medium. It has been revealed that the most important step in preserving primary pancreas and liver cells is selecting the appropriate supplements to support the viability and functionality of these cells.
Methods: Cultivation supplements were prepared by adding bilirubin, hemin, zinc protoporphyrin, glutathione, curcumin, and their combination in the pancreas and liver cell culture at a concentration of 1, 3, and 5 μM. Then, the survival rate and function of the pancreas and liver cells were evaluated. The function of pancreas cells was evaluated based on producing insulin and the function of liver cells was based on liver enzymes, including transaminases.
Results: We found that bilirubin, hemin, zinc protoporphyrin, curcumin, glutathione, and their combination as supplements can dose-dependently maintain pancreas and liver cells viability and functionality proven by increasing insulin secretion levels and transaminase enzyme activity. The strength of effects is displayed in the following order: bilirubin> combination of all compounds> hemin> zinc protoporphyrin> curcumin> glutathione.
Conclusion: This study shows that these compounds are suitable supplements with special biochemical properties. They provide optimal supplements for the culture media of pancreas and liver cells. They may fulfill a function in the antioxidant protection of pancreas and liver cells....
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- Optimization of Culture Condition for the Production of Menaquinone-7 by
Bacillus subtilis natto-
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Authors: Negar Mottaghi-Dastjerdi
Abstract: Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran.
Objectives: Since there was no report on the yield of MK-7 produced by the currently available Bacillus subtilis natto species in Iran, this study aims to optimize the culture condition for the production of MK-7 using this Bacillus species.
Methods: The base medium (BM) for MK-7 production contained glycerol (6.3%), soybean peptone (3%), and yeast extract (0.51%). The selected factors for optimizing the MK-7 production included the incubation temperature (30, 37, and 40°C) and incubation time (72, 96, and 120hr) with/without the addition of K2HPO4 to the fermentation medium. Three sets of experiments with six modes in each set were designed based on these parameters. MK-7 content was analyzed by the HPLC method.
Results: Based on the designed experiments in this study, the highest MK-7 production yield were 0.319 and 0.3158 mg/L. These MK-7 yields were seen with adding K2HPO4 to medium and after 120hr incubation at 30 and 37°C, respectively.
Conclusions: The results showed that Bacillus subtilis natto (IBRC-M 11153) is suitable to be used as a basic platform for mutation and production of a high-producer species. Optimizing the culture conditions using the wild-type species is not beneficial in increasing the production ability of the bacterium. This study would serve as a critical experimental basis for microbial production of MK-7 in Iran. However, it is necessary to use different methods for enhancing the production yield of MK-7 to lower the cost of microbial production and make the industrial process economic.
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- Investigation of seroprevalence of hydatidosis in high-risk individuals in
Sistan and Baluchestan province, southeast of Iran-
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Authors: Shirzad Gholami
Abstract: Background: Hydatidosis is one of the most prevalent zoonotic diseases around the world that is endemic in Iran too. In rural areas, there is a higher risk of infection. To the best of our knowledge, no study was done on seroprevalence of hydatidosis in Sistan and Baluchestan province, southeast of Iran. So, we aimed to investigate the seroprevalence of hydatidosis and its risk factors in high-risk individuals (farmers and ranchers) living in Sistan and Baluchestan province.
Materials and Methods: A total of 500 serum samples were collected. The participants completed a researcher-made questionnaire. Then, the anti-Echinococcus granulosus antibody was analyzed using Counter-current immunoelectrophoresis (CCIEP) and enzyme-linked immunosorbent assay (ELISA) methods. Data analysis was done by applying logistic regression in SPSS (version 22).
Results: Four cases (0.8%) were found positive for anti-Echinococcus granulosus antibody by both CCIEP and ELISA tests. Seroprevalence of hydatidosis was higher in rural people than in urban areas and higher in illiterate people than educated people. Nevertheless, there were no significant differences between seropositivity and gender, age, residence, education, occupation, and contact with dog (P>0.05).
Conclusion: In this study, the prevalence of hydatidosis in Sistan and Baluchestan province was similar to neighboring provinces. The results of the present study on high-risk individuals offer remarkable information about the epidemiology of hydatidosis in Sistan and Baluchestan province in southeastern Iran. This could help to manage and prevent this infection.
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- Molecular Cloning of DARPins G3 in pET28b Expression Vector and
Optimization of Expression of this Protein in E. coli-
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Authors: Shaban Ali Khodashenas
Abstract: Human Epidermal Growth Factor Receptor 2 (HER2) is over-expressed in breast, ovarian, gastric and prostate cancers and is used as a tumor marker in the diagnosis of cancer. Monoclonal antibodies have been used as a diagnostic and therapeutic tool against HER2. Because of the difficulties associated with stability and complexity of the construct and the high cost of antibody production, cloning and expression of HER2-binding DARPins genes to identify HER2-positive tumor markers, we aimed to investigate. After synthesis, the DARPins peptide gene was cloned into the M13 vector and sub-cloned into the TOP10 bacterial vector. After culturing the bacteria on agarose plate containing antibiotics, the transfected bacteria expressing the DARPins gene were selected. To ensure gene cloning, we used enzymatic digestion and recombinant plasmid delivery for sequencing. Isopropyl β-d-1-thiogalactopyranoside (IPTG) was used for the induction of recombinant protein expression and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) method and Western blot for expression confirmation. We were succeeded to express new codon optimized DARPins gene in E.coli and HEK293t system.
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- Designing Multi‐Epitope Subunit Vaccine Candidate for Zika Virus
Utilizing In silico Tools-
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Authors: Azizeh Asadzadeh
Abstract: Background: The arboviruses Zika virus (ZIKV) is a pathogen that threatens human health. Scientists have warned that a single mutation in the mosquito-borne Zika virus could spark another major outbreak of the disease in humans, Therefore, designing a suitable vaccine for this virus seems necessary. This study aimed to predict the protective epitopes of envelope protein from the Zika virus with bioinformatics methods for multi-epitope vaccine development.
Materials and Methods: Computational studies including identification of potential B-cell and T-cell epitopes were used For the generation of a multi-epitopic vaccine construct (MEVC), selected epitopes are connected together by suitable linkers. In order to enhance protein immunogenicity, Maltose-bound protein (MBP) was added to the MEVC, after prediction and refinement 3D structure of the designed vaccine, binding mode of the MEVC with toll-like-receptor (TLR4), was investigated by molecular docking technique. Finally, molecular dynamics and in silico cloning was performed for designed vaccine.
Results: This study showed that this recombinant vaccine is nontoxic, nonallergenic, and thermostable and elicits immune responses against the Zika virus.
Conclusion: The computational data suggest that the MEVC has appropriate characteristics and a high-quality structure.
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- The frequency of human papillomaviruses and Epstein–Barr virus in
colorectal cancer samples in Algeria-
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Authors: dahmani bouchra
Abstract: Background: environmental factors may play a role in colon cancer. In this view, several studies investigated tumor samples for the presence of various viral DNA with conflictiong results.
The purpose of this study is to investigate the prevalence of Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) in patients with colorectal carcinomas.
Materials and methods: In this study, we collected 74 tumorous paraffin-embedded tissues (mean age 66,3 ± 14,98) from the Pathology Department of The hospital of Ain temouchent and laboratories of pathological anatomy of western of Algeria. DNA from each tissue was extracted and the presence of HPV and EBV was investigated using PCR.
Results: none of our samples were HPV or EBV positive, and we failed to find an obvious correlation between EBV and HPV infections and this type of cancer.
Conclusion : the results suggested that EBV and HPV infection is not common in patients with colorectal cancer in our population. however, the findings merit more investigations on a large number of cases.
Background: environmental factors may play a role in colon cancer. In this view, several studies investigated tumor samples for the presence of various viral DNA with conflictiong results.
The purpose of this study is to investigate the prevalence of Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) in patients with colorectal carcinomas.
Materials and methods: In this study, we collected 74 tumorous paraffin-embedded tissues (mean age 66,3 ± 14,98) from the Pathology Department of The hospital of Ain temouchent and laboratories of pathological anatomy of western of Algeria. DNA from each tissue was extracted and the presence of...
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