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Showing 1 - 200 of 3120 Journals sorted alphabetically
A Practical Logic of Cognitive Systems     Full-text available via subscription   (Followers: 8)
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 26, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 22, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 90, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 25, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 30, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 378, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 26, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 1)
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (Followers: 1, SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 237, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 10, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 25, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 4, SJR: 0.383, h-index: 19)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 2)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 5)
Acute Pain     Full-text available via subscription   (Followers: 13)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 7)
Additive Manufacturing     Hybrid Journal   (Followers: 7, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 22)
Advanced Cement Based Materials     Full-text available via subscription   (Followers: 3)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 138, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 17, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 27, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 4)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 9, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 12, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 23, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 26, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 6, SJR: 2.139, h-index: 42)
Advances in Cell Aging and Gerontology     Full-text available via subscription   (Followers: 4)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 13)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 26, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 9, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 29, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Dermatology     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 12)
Advances in Digestive Medicine     Open Access   (Followers: 7)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 6)
Advances in Drug Research     Full-text available via subscription   (Followers: 23)
Advances in Ecological Research     Full-text available via subscription   (Followers: 47, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 27, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 9)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 46, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 52, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 16)
Advances in Genetics     Full-text available via subscription   (Followers: 17, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 22, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 27)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 36, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 6)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 10)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 5, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 23)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 9, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 2)
Advances in Organ Biology     Full-text available via subscription   (Followers: 2)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 24, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 16, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 7)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 5)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20, SJR: 1.5, h-index: 62)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 6, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 3, SJR: 0.1, h-index: 2)
Advances in Space Biology and Medicine     Full-text available via subscription   (Followers: 5)
Advances in Space Research     Full-text available via subscription   (Followers: 371, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 9, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 31, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 16)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 6, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 45, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 339, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 6, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 9, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 432, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 31, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 42, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 3)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 56, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 11, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 8)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access   (Followers: 1)
Algal Research     Partially Free   (Followers: 9, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
Alpha Omegan     Full-text available via subscription   (SJR: 0.121, h-index: 9)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 9, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 49, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 4)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 4)
Ambulatory Pediatrics     Hybrid Journal   (Followers: 5)
American Heart J.     Hybrid Journal   (Followers: 48, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 48, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 42, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 9, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 14, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 32, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 26, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 31, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 45, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 207, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 61, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 6)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 24, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 27, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 26, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 36, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 6)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 60, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 14)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 4, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 36, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 171, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 12)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 1)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 22, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 176, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)

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Journal Cover Acta Histochemica
  [SJR: 0.604]   [H-I: 38]   [3 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0065-1281
   Published by Elsevier Homepage  [3123 journals]
  • Antioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can
           protect human sperm deoxyribonucleic acid from oxidative damage
    • Authors: Şule Ayla; Gülden Tunalı; Bülent E. Bilgiç; Kenan Sofuoğlu; A.Arman Özdemir; Gamze Tanrıverdi; Semra Özdemir; B.Cem Soner; Bahar Öztürk; Serçin Karahüseyinoğlu; Esra Güler Aslan; Ismail Seçkin
      Abstract: Publication date: Available online 8 January 2018
      Source:Acta Histochemica
      Author(s): Şule Ayla, Gülden Tunalı, Bülent E. Bilgiç, Kenan Sofuoğlu, A.Arman Özdemir, Gamze Tanrıverdi, Semra Özdemir, B.Cem Soner, Bahar Öztürk, Serçin Karahüseyinoğlu, Esra Güler Aslan, Ismail Seçkin
      Purpose Sperm processing (e.g., centrifugation) used in preparation for assisted reproduction can result in excessive generation of reactive oxygen species (ROS) and potential sperm damage. The use of antioxidants during sperm processing has been shown to prevent iatrogenic sperm damage, including DNA damage. In this study, we evaluated the effect of caffeic acid phenethyl ester (CAPE) on oxidative stress mediated sperm dysfunction and DNA damage. Methods Semen samples were obtained to liquefy at room temperature. After centrifugation and washing protocols, spermatozoa were incubated in a single step supplemented medium with either of 10, 50 or 100 μmol/L CAPE for 2 hours at 36 °C. After incubation period, MDA levels of seminal plasma were measured. The fragmentation in sperm DNA was detected by light microscopy via use of an aniline blue assay, while ultrastructural morphology was analyzed by transmission electron microscopy. Results Significant increase has been observed in percent chromatin condensation (assessed by aniline blue staining) and Malondialdehyde (Mmol/L) in oligoasthenoteratozoospermia group before the centrifugation (0.57 ± 0.15). Incubation of samples with 100 μmol/L CAPE after centrifugation resulted in a significantly lower percent chromatin condensation compared to samples incubated without CAPE (0.42 ± 0.12) (P < 0.0033). Incubation of all samples with CAPE (10 μmol/L, 50 μmol/L, 100 μmol/L.) after centrifugation resulted in a significantly lower percentage of Malondialdehyde levels. Conclusions The data suggests that preincubation of spermatozoa with the antioxidant CAPE offers protection against oxidative DNA damage in vitro.

      PubDate: 2018-01-09T20:24:15Z
      DOI: 10.1016/j.acthis.2018.01.001
  • Retraction notice to Polar and apolar extra virgin olive oil and leaf
           extracts as a promising anti-inflammatory natural treatment for
           osteoarthritis. ACTHIS_119_4 (2017) 407-416
    • Authors: Houda Nsir; Marta Anna Szychlinska; Venera Cardile; Adriana Carol Eleonora Graziano; Rosanna Avola; Hanen Esafi; Alessandra Bendini; Mokhtar Zarouk; Carla Loreto; Venerando Rapisarda; Paola Castrogiovanni; Giuseppe Musumeci
      First page: 64
      Abstract: Publication date: January 2018
      Source:Acta Histochemica, Volume 120, Issue 1
      Author(s): Houda Nsir, Marta Anna Szychlinska, Venera Cardile, Adriana Carol Eleonora Graziano, Rosanna Avola, Hanen Esafi, Alessandra Bendini, Mokhtar Zarouk, Carla Loreto, Venerando Rapisarda, Paola Castrogiovanni, Giuseppe Musumeci

      PubDate: 2017-12-26T20:55:40Z
      DOI: 10.1016/j.acthis.2017.08.001
  • The effects of knee immobilization on marrow adipocyte hyperplasia and
           hypertrophy at the proximal rat tibia epiphysis
    • Authors: Guy Trudel; Hans K. Uhthoff; Sanjay Solanki; Odette Laneuville
      Pages: 759 - 765
      Abstract: Publication date: September 2017
      Source:Acta Histochemica, Volume 119, Issue 7
      Author(s): Guy Trudel, Hans K. Uhthoff, Sanjay Solanki, Odette Laneuville
      Marrow adipose deposition is observed during aging and in association with extended periods of immobility. The objective of this study was to determine the contribution of adipocyte hypertrophy and hyperplasia to bone marrow fat deposition induced by immobilization of the rat knee joint for 2, 4, 16 or 32 weeks. Histomorphometric analyses compared immobilized to sham-operated proximal tibia from age and gender matched rats to assess the contribution of aging and duration of immobilization on the number and size of marrow adipocytes. Results indicated that marrow adipose tissue increased with the duration of immobilization and was significant larger at 16 weeks compared to the sham-operated group (0.09956±0.13276mm2 vs 0.01990±0.01100mm2, p=0.047). The marrow adipose tissue was characterized by hyperplasia of adipocytes with a smaller average size after 2 and 4 weeks of immobilization (at 2 weeks hyperplasia: 68.86±33.62 vs 43.57±24.47 adipocytes/mm2, p=0.048; at 4 weeks hypotrophy: 0.00036±0.00019 vs 0.00046±0.00023mm2, p=0.027), and by adipocyte hypertrophy after 16 weeks of immobilization (0.00083±0.00049 vs 0.00046±0.00028mm2, p=0.027) compared to sham-operated. Both immobilized and sham-operated groups showed marrow adipose conversion with age; immobilized (p=0.008; sham: p=0.003). Overall, fat deposition in the bone marrow of the proximal rat tibia epiphysis and induced by knee joint immobilization was characterized by hyperplasia of small adipocytes in the early phase and by adipocyte hypertrophy in the later phase. Mediators of marrow fat deposition after immobilization and preventive countermeasures need to be investigated.

      PubDate: 2017-09-30T18:44:05Z
      DOI: 10.1016/j.acthis.2017.09.008
  • Distribution of nerve fibers during the development of palatine glands in
    • Authors: Zaki Hakami
      Abstract: Publication date: Available online 26 December 2017
      Source:Acta Histochemica
      Author(s): Zaki Hakami
      Background Salivary gland maturation and function are modulated by the nervous system. Nevertheless, little is known about salivary gland innervation during development, particularly minor salivary glands. This study investigated the development of the innervation of the palatine glands of rat. Materials and methods Frozen sections of rat palatine glands at different stages were immunohistochemically labeled for detection of the general nerve markers protein gene product 9.5 (PGP 9.5) and growth associated protein 43 (GAP-43), and the autonomic nerve markers calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY). Results PGP 9.5 and GAP-43-immunoreactive fibers (IRF) were present in the mesenchyme and in association with developing acini, ducts and blood vessels. GAP-43-IRF were more abundant and diffuse than PGP 9.5-IRF at early stages, but showed similar distribution with growth, ramifying out from thick bundles in connective tissues until encircling the secretory units observed around postnatal day 21 (PN21). CGRP-IRF were detected in the mesenchyme at embryonic day 20 (E20) and PN0. CGRP-IRF became numerous around PN7 and PN10. They then decreased to the adult level at PN21, mainly located around ducts and infrequently blood vessels. NPY-IRF were sparsely detected in the mesenchyme at E20, then detected in close proximity to acini in addition to blood vessels at PN3. NPY-IRF increased till reaching the adult stage, and were mainly associated with blood vessels and around mucous cells and some serous demilunes. Conclusion The findings indicated a developmental modification of the sensory and autonomic innervation which may play a role in the functional maturation of the palatine salivary glands.

      PubDate: 2017-12-26T20:55:40Z
      DOI: 10.1016/j.acthis.2017.12.007
  • Diabetes mellitus- induction: Effect of different streptozotocin doses on
           male reproductive parameters
    • Authors: Temidayo S. Omolaoye; Bongekile T. Skosana; Stefan S. du Plessis
      Abstract: Publication date: Available online 24 December 2017
      Source:Acta Histochemica
      Author(s): Temidayo S. Omolaoye, Bongekile T. Skosana, Stefan S. du Plessis
      Diabetes mellitus (DM) is reported to be involved in male reproductive impairment, and its impact is evident in the increased prevalence of infertility. Various studies have reported that a single parenteral injection of <40 mg/kg Streptozotocin (STZ) is ineffective in ablating pancreatic β-cells and creating a rat model to investigate the effect of DM on the male reproductive system. This study therefore aims to validate these claims. Adult male Wistar rats received either a single intraperitoneal injection of STZ (30 mg/kg or 60 mg/kg) or saline (0.9%, Control). Diabetes was confirmed after 72 h if plasma glucose levels were ≥14 mmol/L. Body weight, glucose level, fluid and food intake were measured weekly. Animals were sacrificed after 8 weeks of treatment by an overdose of sodium pentobarbital (160 mg/kg body weight). The testis and epididymis were harvested and weighed prior to preparation for histological evaluation. Epididymal sperm morphology was analysed using computer aided sperm analysis (CASA). STZ60 animals presented with significantly lower body weights compared to both control and STZ30 groups. Animals in both STZ30 and STZ60 groups showed decreased normal sperm morphology compared to control. Histological evaluation of the testes showed a decrease in the number of spermatozoa in the seminiferous tubules of animals in the STZ30 and STZ60 groups compared to control. A complete absence of spermiogenesis was observed in the seminiferous tubules of STZ60 animals. These findings prove that an STZ concentration of 30 mg/kg, which is much lower than the reported 40 mg/kg, has adverse effects on the male reproductive system via its diabetogenic effect and can therefore be used to study the impact of DM on male fertility.

      PubDate: 2017-12-26T20:55:40Z
      DOI: 10.1016/j.acthis.2017.12.005
  • MiR-199-3p replacement affects E-cadherin expression through Notch1
           targeting in hepatocellular carcinoma
    • Authors: Catia Giovannini; Francesca Fornari; Rossella Dallo; Martina Gagliardi; Elisa Nipoti; Francesco Vasuri; Camelia Alexandra Coadă; Matteo Ravaioli; Luigi Bolondi; Laura Gramantieri
      Abstract: Publication date: Available online 15 December 2017
      Source:Acta Histochemica
      Author(s): Catia Giovannini, Francesca Fornari, Rossella Dallo, Martina Gagliardi, Elisa Nipoti, Francesco Vasuri, Camelia Alexandra Coadă, Matteo Ravaioli, Luigi Bolondi, Laura Gramantieri
      Hepatocellular carcinoma (HCC) represents the second cause of cancer-related mortality worldwide and is associated with poor prognosis, due to a high recurrence rate after curative treatments and a drug resistance phenotype. In this scenario, the identification of innovative and effective therapeutic strategies is an unmet clinical need. The safety and efficacy of microRNA (miRNA) mediated approaches in preclinical models and clinical trials have been widely described in cancer. MicroRNA-199a downregulation is a common feature of HCC where its reduced expression contributes to mTOR and c-Met pathways activation. Notch1 activation is also a common event in HCC, influencing epithelial-to-mesenchymal transition, tumor invasion and recurrence at least in part through E-cadherin regulation. Here we identified a negative correlation between miR-199a-3p and Notch1 or E-cadherin protein levels in HCC patients and demonstrated that miR-199a-3p regulates E-cadherin expression through Notch1 direct targeting in in vitro models. Moreover, we showed that a strong correlation exists between miR-199a-5p and miR-199a-3p in HCC specimens and that miR-199a-5p contributes to E-cadherin regulation as well, underlying the complex network of interaction carried out by miR-199a and its influence on tumor aggressiveness. In conclusion, our findings suggest the restoration of miR-199a-3p physiologic levels as a possible therapeutic strategy for the treatment of HCC.
      Graphical abstract image

      PubDate: 2017-12-26T20:55:40Z
      DOI: 10.1016/j.acthis.2017.12.004
  • Confirmation of the immunoreactivity of monoclonal anti-human C-terminal
           EGFR antibodies in bronze Corydoras Corydoras aeneus (Callichthyidae
           Teleostei) by Western Blot method
    • Authors: Jennifer Mytych; Leszek Satora; Katarzyna Kozioł
      Abstract: Publication date: Available online 13 December 2017
      Source:Acta Histochemica
      Author(s): Jennifer Mytych, Leszek Satora, Katarzyna Kozioł
      Bronze corydoras (Corydoras aeneus) uses the distal part of the intestine as accessory respiratory organ. Our previous study showed the presence of epidermal growth factor receptor (EGFR) cytoplasmic domain in the digestive tract of the bronze corydoras. In this study, using Western Blot method, we validated the results presented in the previous research. In detail, results of Western Blot analysis on digestive and respiratory part of bronze corydoras intestine homogenates confirmed the immunoreactivity of anti-cytoplasmic domain (C-terminal) human EGFR antibodies with protein band of approximately 180kDa (EGFR molecular weight). This indicates a high homology of EGFR domain between these species and the possibility of such antibody use in bronze corydoras. Statistically significantly higher EGFR expression was observed in the respiratory part of intestine when compared to the digestive part. This implies higher proliferation activity and angiogenesis of epithelium in this part of intestine, creating conditions for air respiration. Therefore, Corydoras aeneus may be considered as a model organism for the molecular studies of the mechanisms of epithelial proliferation initiation and inhibition depending on hypoxia and normoxia.

      PubDate: 2017-12-13T04:45:23Z
      DOI: 10.1016/j.acthis.2017.12.002
  • Polyploidy and nuclear phenotype characteristics of cardiomyocytes from
           diabetic adult and normoglycemic aged mice
    • Authors: Isabela S. Silva; Flávia G. Ghiraldini; Giovana M.B. Veronezi; Maria Luiza S. Mello
      Abstract: Publication date: Available online 12 December 2017
      Source:Acta Histochemica
      Author(s): Isabela S. Silva, Flávia G. Ghiraldini, Giovana M.B. Veronezi, Maria Luiza S. Mello
      The frequency of polyploid nuclei in the aging human heart is in sharp contrast with that in the human liver. An inverse pattern exists between the mouse heart and liver cells. Ploidy degrees in mouse hepatocytes under hyperglycemic conditions are elevated to higher levels than those in aged hepatocytes. In this study, image analysis cytometry was used to investigate the effect of diabetes and aging on Feulgen-DNA quantities, ploidy degrees, nuclear shapes and chromatin texture in mouse cardiomyocytes compared to previously reported data for mouse hepatocytes. Adult, non-obese diabetic (NOD) hyperglycemic and normoglycemic females and 56-week-old normoglycemic BALB/c females were used. A small percentage (∼7%) of the cardiomyocyte nuclei in severely hyperglycemic NOD adult mice possessed higher ploidy values than those in the 8-week-old normoglycemic mice. Surprisingly, the Feulgen-DNA values and the frequency of nuclei belonging to the 4C and 8C ploidy classes were even higher (∼6%) in normoglycemic NOD specimens than in age-matched hyperglycemic NOD specimens. Additionally, a pronounced elongated nuclear shape was observed especially in adult normoglycemic NOD mice. In conclusion, NOD mice, irrespective of their glycemic level, exhibit a moderate increase in ploidy degrees within cardiomyocyte nuclei during the adult lifetime. As expected, aging did not affect the Feulgen-DNA values and the ploidy degrees of cardiomyocytes in BALB/c mice. The differences in ploidy degrees and chromatin textures such as absorbance variability and entropy, between adult NOD and aged BALB/c mice are consistent with other reports, indicating dissimilarities in chromatin functions between diabetes and aging.

      PubDate: 2017-12-13T04:45:23Z
      DOI: 10.1016/j.acthis.2017.12.003
  • Neurons and satellite glial cells in adult rat lumbar dorsal root ganglia
           express connexin 36
    • Authors: E. Martha Pérez Armendariz; Monica Norcini; Beatriz Hernández-Tellez; Andrés Castell-Rodríguez; Cristina Coronel-Cruz; Raquel Guerrero Alquicira; Alexandra Sideris; Esperanza Recio-Pinto
      Abstract: Publication date: Available online 8 December 2017
      Source:Acta Histochemica
      Author(s): E. Martha Pérez Armendariz, Monica Norcini, Beatriz Hernández-Tellez, Andrés Castell-Rodríguez, Cristina Coronel-Cruz, Raquel Guerrero Alquicira, Alexandra Sideris, Esperanza Recio-Pinto
      Previous studies have shown that following peripheral nerve injury there was a downregulation of the gap junction protein connexin 36 (Cx36) in the spinal cord; however, it is not known whether Cx36 protein is expressed in the dorsal root ganglia (DRGs), nor if its levels are altered following peripheral nerve injuries. Here we address these aspects in the adult rat lumbar DRG. Cx36 mRNA was detected using qRT-PCR, and Cx36 protein was identified in DRG sections using immunohistochemistry (IHC) and immunofluorescence (IF). Double staining revealed that Cx36 co-localizes with both anti-β-III tubulin, a neuronal marker, and anti-glutamine synthetase, a satellite glial cell (SGC) marker. In neurons, Cx36 staining was mostly uniform in somata and fibers of all sizes and its intensity increased at the cell membranes. This labeling pattern was in contrast with Cx36 IF dots mainly found at junctional membranes in islet beta cells used as a control tissue. Co-staining with anti-Cx43 and anti-Cx36 showed that whereas mostly uniform staining of Cx36 was found throughout neurons and SGCs, Cx43 IF puncta were localized to SGCs. Cx36 mRNA was expressed in normal lumbar DRG, and it was significantly down-regulated in L4 DRG of rats that underwent sciatic nerve injury resulting in persistent hypersensitivity. Collectively, these findings demonstrated that neurons and SGCs express Cx36 protein in normal DRG, and suggested that perturbation of Cx36 levels may contribute to chronic neuropathic pain resulting from a peripheral nerve injury.

      PubDate: 2017-12-12T04:44:25Z
      DOI: 10.1016/j.acthis.2017.11.005
  • Effects of acrylamide on oxidant/antioxidant parameters and CYP2E1
           expression in rat pancreatic endocrine cells
    • Authors: Jelena Marković; Milena Stošić; Danijela Kojić; Milica Matavulj
      Abstract: Publication date: Available online 8 December 2017
      Source:Acta Histochemica
      Author(s): Jelena Marković, Milena Stošić, Danijela Kojić, Milica Matavulj
      Oxidative stress is one of the principle mechanism of acrylamide-induced toxicity. Acrylamide is metabolized by cytochrome P450 2E1 (CYP2E1) to glycidamide or by direct conjugation with glutathione. Bearing in mind that up to now the effects of acrylamide on oxidative stress status and CYP2E1 level in endocrine pancreas have not been studied we performed qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), catalase (CAT) and CYP2E1 expression in islets of Langerhans of rats subchronically treated with 25 or 50mg/kg bw of acrylamide. Since the majority of cells (>80%) in rodent islets are beta cells, in parallel studies, we employed the Rin-5F beta cell line to examine effects of acrylamide on redox status and the activity of CAT, SOD and glutathione-S-transferase (GST), their gene expression, and CYP2E1, NF-E2 p45-related factor 2 (Nrf2) and iNOS expression. Immunohistochemically stained pancreatic sections revealed that acrylamide induced increase of iNOS and decrease of CYP2E1 protein expression, while expression of antioxidant enzymes was not significantly affected by acrylamide in islets of Langerhans. Analysis of Mallory-Azan stained pancreatic sections revealed increased diameter of blood vessels lumen in pancreatic islets of acrylamide-treated rats. Increase in the GST activity, lipid peroxidation and nitrite level, and decrease in GSH content, CAT and SOD activities was observed in acrylamide-exposed Rin-5F cells. Level of mRNA was increased for iNOS, SOD1 and SOD2, and decreased for GSTP1, Nrf2 and CYP2E1 in acrylamide-treated Rin-5F cells. This is the first report of the effects of acrylamide on oxidant/antioxidant parameters and CYP2E1 expression in pancreatic endocrine cells.

      PubDate: 2017-12-12T04:44:25Z
      DOI: 10.1016/j.acthis.2017.12.001
  • The underlying physiological basis of the desert rodent Meriones shawi's
           survival to prolonged water deprivation: Central vasopressin regulation on
           peripheral kidney water channels AQPs-2
    • Authors: A. Elgot; O. El Hiba; M. Belkouch; H. Gamrani
      Abstract: Publication date: Available online 6 December 2017
      Source:Acta Histochemica
      Author(s): A. Elgot, O. El Hiba, M. Belkouch, H. Gamrani
      Meriones shawi (M. shawi) is a particular semi-desert rodent known by its resistance to long periods of thirst. The aim of the present investigation is to clarify the underlying mechanisms allowing M. shawi to resist to hard conditions of dehydration. For this reason we used two different approaches: i) a morphometric study, which consists in measuring the effect of dehydration on body and kidneys weights as well as the report kidney weight/body weight, ii) By immunohistochemistry, we proceed to study the effect of dehydration on the immunoreactivity of central vasopressin (AVP) and the kidney aquaporin-2 (AQP-2) which is a channel protein that allows water to permeate across cell membranes. Our results showed both a body mass decrease accompanied by a remarkable kidneys hypertrophy. The immunohistochemical study showed a significant increase of AQP-2 immunoreactivity in the medullar part of Meriones kidneys allowing probably to Meriones a great ability to water retention. Consistently, we demonstrate that the increased AQP-2 expression occurred together with an increase in vasopressin (AVP) expression in both hypothalamic supraoptic (SON) and paraventricular nucleus (PVN), which are a major hub in the osmotic control circuitry. These various changes seen either in body weight and kidneys or at the cellular level might be the basis of peripheral control of body water homeostasis, providing to M. shawia strong resistance against chronic dehydration.

      PubDate: 2017-12-12T04:44:25Z
      DOI: 10.1016/j.acthis.2017.11.006
  • Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction
           and inhibits EMT program of colorectal cancer cells
    • Authors: Guodong Liang; Xuedong Fang; Yubo Yang; Yan Song
      Abstract: Publication date: Available online 22 November 2017
      Source:Acta Histochemica
      Author(s): Guodong Liang, Xuedong Fang, Yubo Yang, Yan Song
      Cell migration inducing hyaluronan binding protein (CEMIP) is a hyaluronic acid binding protein, the abnormal elevation of which is suggested as a contributor in the carcinogenesis of colorectal cancer (CRC). Cancer cells lose their adhesive properties and acquire an enhanced mobility by undergoing epithelial-mesenchymal transition (EMT). This study is performed to investigate whether and how CEMIP orchestrates the EMT process of CRC cells. To avoid the unexpected off-target effects possibly caused by one single shRNA, two shRNAs targeting different mRNA regions of CEMIP gene were used to knock down the mRNA and protein expression of CEMIP. Our data showed that the proliferation, migration and invasion of two CRC cell lines, HCT116 and SW480 cells, were inhibited by CEMIP shRNA. We here defined EMT as the complete or partial loss of E-cadherin and zona occludens protein 1 (ZO-1) (epithelial markers) and the gain of Vimentin and N-cadherin (mesenchymal markers), and found that the EMT process was attenuated in CEMIP-silenced SW480 cells. Snail, a direct target of β-catenin/T cell factor complex, is known to activate the EMT program during cancer metastasis. CEMIP shRNA was further found to suppress the Wnt/β-catenin/Snail signaling transduction in CRC cells as manifested by the decreased nuclear β-catenin and Snail. Collectively, our work demonstrates that CEMIP contributes to metastatic phenotype of CRC cells in vitro.

      PubDate: 2017-12-01T04:35:31Z
      DOI: 10.1016/j.acthis.2017.11.002
  • Immunohistochemical analysis of the distribution of molecules involved in
           ionic and pH regulation in the lancelet Branchiostoma floridae (Hubbs,
    • Authors: Ivan Cuoghi; Clara Lazzaretti; Mauro Mandrioli; Lucrezia Mola; Aurora Pederzoli
      Abstract: Publication date: Available online 21 November 2017
      Source:Acta Histochemica
      Author(s): Ivan Cuoghi, Clara Lazzaretti, Mauro Mandrioli, Lucrezia Mola, Aurora Pederzoli
      The aim of present work is to analyse the distribution of carbonic anhydrase II (CAII), cystic fibrosis transmembrane regulator (CFTR), vacuolar-type H+-ATPase (V-H+-ATPase), Na+/K+ ATPase, Na+/H+ exchanger (NHE) and SLC26A6 (solute carrier family 26, member 6), also known as pendrin protein, in the lancelet Branchiostoma floridae in order to go in depth in the evolution of osmoregulation and pH regulation in Chordates. In view of their phylogenetic position, lancelets may indeed provide a critical point of reference for studies on osmoregulation evolution in Chordates. The results of present work demonstrated that, except to Na+/K+ ATPase that is strongly expressed in nephridia only, all the other studied molecules are abundantly present in skin, coelomic epithelium, renal papillae and nephridia and hepatic coecum. Thus, it is possible to hypothesize that also in lancelet, as in fish, these organs are involved in pH control and ionic regulation. In the digestive tract of B. floridae, the intestine epithelium was weakly immune-reactive to all tested antibodies, while the hepatic coecum showed an intense immunoreactivity to all molecules. Since in amphioxus the hepatic coecum functions simultaneously as stomach, liver and pancreas, these immunohistochemical results proved the secretion of H+ and HCO3 − ions, typical of digestive process. Colocalization studies indicated a co-expression of the studied proteins in all considered organs, excluding NHE and pendrin for renal papillae, since some renal papillae are NHE immunopositive only.

      PubDate: 2017-12-01T04:35:31Z
      DOI: 10.1016/j.acthis.2017.10.011
  • Vitamin E can improve behavioral tests impairment, cell loss, and dendrite
           changes in rats’ medial prefrontal cortex induced by acceptable daily
           dose of aspartame
    • Authors: Ali Rafati; Ali Noorafshan; Mahboubeh Jahangir; Leila Hosseini; Saied Karbalay-Doust
      Abstract: Publication date: Available online 21 November 2017
      Source:Acta Histochemica
      Author(s): Ali Rafati, Ali Noorafshan, Mahboubeh Jahangir, Leila Hosseini, Saied Karbalay-Doust
      Aspartame is an artificial sweetener used in about 6000 sugar-free products. Aspartame consumption could be associated with various neurological disorders. This study aimed to evaluate the effect of aspartame onmedial Prefrontal Cortex (mPFC) as well as neuroprotective effects of vitamin E. The rats were divided into seven groups, including distilled water, corn oil, vitamin E (100mg/kg/day), and low (acceptable daily dose) and high doses of aspartame (40 and 200mg/kg/day) respectively, with or without vitamin E consumption, for 8 weeks. Behavioral tests were recorded and the brain was prepared for stereological assessments. Novel objects test and eight-arm radial maze showed impairmentoflong- and short-termmemoriesin aspartame groups. Besides, mPFC volume, infralimbic volume, neurons number, glial cells number, dendrites length per neuron,and number of spines per dendrite length were decreased by 7–61% in the rats treated with aspartame. However, neurons’ number, glial cells number, and rats’ performance in eight-arm radial mazes were improved by concomitant consumption of vitamin E and aspartame. Yet, the mPFC volume and infralimbic cortex were protected only in the rats receiving the low dose of aspartame+vitamin E. On the other hand, dendrites length, spines number,and novel object recognition were not protected by treatment with vitamin E+aspartame. The acceptable daily dose or higher doses of aspartame could induce memory impairments and cortical cells loss in mPFC. However, vitamin E could ameliorate some of these changes.

      PubDate: 2017-12-01T04:35:31Z
      DOI: 10.1016/j.acthis.2017.11.004
  • Specific localization of manserin peptide in the rat carotid body
    • Authors: Michiru Ida-Eto; Takeshi Ohkawara; Masaaki Narita
      Abstract: Publication date: Available online 21 November 2017
      Source:Acta Histochemica
      Author(s): Michiru Ida-Eto, Takeshi Ohkawara, Masaaki Narita
      The carotid body, located at the bifurcation of the common carotid artery, is a small sensory organ that detects changes in oxygen concentration and plays a vital role in controlling respiration. Although several molecules, such as neurotransmitters and neuropeptides, are involved in the regulation of the respiratory system, their detailed mechanisms have not been established yet. This study identifies that the presence of manserin, a neuropeptide, in the carotid body may play a crucial role in regulating respiration. The carotid bodies of adult Wistar rats were perfused with paraformaldehyde, and the frozen sections were subjected to immunohistochemical analyses. The carotid body comprises two distinct types of cells, neuron-like glomus cells and glial-like sustentacular cells. We used specific antibodies to distinguish the specific location of manserin in the carotid body, which included a tyrosine hydroxylase-positive antibody for glomus cells and an S100 protein antibody for sustentacular cells. Immunofluorescence analysis revealed that while tiny, round signals were exclusively observed in the cytoplasm of glomus cells, no signals were observed in sustentacular cells. Because manserin is believed to be secreted from precursor proteins by the endoproteolytic processing of a large precursor protein called secretogranin II, manserin secretion systems may exist in the carotid body, and thus, behave as potential regulators of respiration in the carotid body.

      PubDate: 2017-12-01T04:35:31Z
      DOI: 10.1016/j.acthis.2017.10.006
  • The molecular phenotypes of ureteral telocytes are layer-specific
    • Authors: M.A. Dobra; A.D. Vrapciu; F. Pop; N. Petre; M.C. Rusu
      Abstract: Publication date: Available online 16 November 2017
      Source:Acta Histochemica
      Author(s): M.A. Dobra, A.D. Vrapciu, F. Pop, N. Petre, M.C. Rusu
      Telocytes (TC) are the delicate interstitial (stromal) cells defined by their long, thin and moniliform processes termed telopodes. Numerous studies determined that different subsets of telocytes populate almost all tissues and attempted to relate these subsets to various functions, from cell signaling to tissue repair and regeneration. Extremely few studies addressed the urinary tract though few data on the molecular pattern of the urinary TCs actually exist. We therefore hypothesized that subsets of urinary TCs co-localize within the human ureter and we aimed at performing an immunohistochemical study to evaluate the tissue-specific molecular pattern of TCs. On sample tissues of proximal ureter drawn from ten human adult patients during surgery were applied primary antibodies against CD34, CD105, von Willebrand Factor, the heavy chain of smooth muscle myosin (SMM) and c-erbB-2. The molecular pattern indicated three different subsets of ureteral TCs which are neither endothelial nor epithelial in nature: (a) type I: the CD34-/CD105+ TCs of the superficial layer of lamina propria; (b) type II: the CD34+/CD105± myoid TCs of the deep layer of lamina propria and (c) type III: the CD34+/CD105+ perivascular TCs. Although apparently different, all these subsets of TCs could belong to the stem/progenitor niche of the ureter.

      PubDate: 2017-12-01T04:35:31Z
      DOI: 10.1016/j.acthis.2017.11.003
  • Bone marrow adipocytes in haematological malignancies
    • Authors: Ewa Frączak; Mateusz Olbromski; Aleksandra Piotrowska; Natalia Glatzel-Plucińska; Piotr Dzięgiel; Jarosław Dybko; Kazimierz Kuliczkowski; Tomasz Wróbel
      Abstract: Publication date: Available online 14 November 2017
      Source:Acta Histochemica
      Author(s): Ewa Frączak, Mateusz Olbromski, Aleksandra Piotrowska, Natalia Glatzel-Plucińska, Piotr Dzięgiel, Jarosław Dybko, Kazimierz Kuliczkowski, Tomasz Wróbel
      Bone marrow adipocytes (BMAs) derived from mesenchymal stem cells (MSC) are an active and significant element of the bone marrow microenvironment. They are involved in metabolic functions, complex interactions with other stromal cells, and in the development and progression of tumours. Currently, there is little data regarding the role of BMAs in haematological malignancies. Due to this, we have attempted to characterise the BMAs in these malignancies in terms of quantity and morphology. Our study included 30 patients aged 22–76 with myelo- (n=17) and lymphoproliferative malignancies (n=13), both with and without bone marrow infiltration. Trepanobioptate was the evaluated material. The number and diameter of BMAs were measured, and the percentage of adipocytes (adipocyte fraction – AF), hematopoietic cells (hematopoietic fraction – HF) and trabecular bone (trabecular bone fraction – BF) was calculated. The obtained results were considered against the clinical parameters of age, sex, body weight, body surface area (BSA) and body mass index (BMI). We observed that as age increases, the number of BMA/mm2, the diameter of adipocytes and AF increase while BF and HF decrease. However, this relationship was not statistically significant. A significant correlation of BMA parameters was also not found in relation to weight, BMI and BSA, and the number and diameter of BMAs were comparable in both sexes. The trepanobioptate of infiltrated bone marrow showed a decreased number of BMA/mm2 compared to the trepanobioptate from bone marrow without infiltration (97.44±69.16 vs. 164.14±54.16; p=0.010) with a marked difference in men (69.75±65.26 vs. 180.33±60.40; p=0.007). These trepanobioptate also showed an increase in the number of BMA/mm2 with age (r=0.472; p=0.041), and with an increase of BMI, an increase in diameter of BMAs (r=0.625; p=0.007) and AF (r=0.546; p=0.023). The number and size of BMAs, as well as AF, BF and HF in patients with myeloproliferative malignancies did not differ significantly compared to patients with lymphoproliferative malignancies.

      PubDate: 2017-11-20T03:10:35Z
      DOI: 10.1016/j.acthis.2017.10.010
  • Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice
    • Authors: Xuexian Liu; Pengqian Zhang; Kaiyuan Ji; Junzhen Zhang; Shanshan Yang; Bin Du; Shuaipeng Hu; Ruiwen Fan
      Abstract: Publication date: Available online 11 November 2017
      Source:Acta Histochemica
      Author(s): Xuexian Liu, Pengqian Zhang, Kaiyuan Ji, Junzhen Zhang, Shanshan Yang, Bin Du, Shuaipeng Hu, Ruiwen Fan
      Cyclin-dependent kinase 5 (CDK5) is a proline-directed serine/threonine kinase that has been shown to play important roles in many tissues except the nervous system. We previously reported that CDK5 showed differential expression in the transcriptome profiles of the skin of alpacas with different hair colors. To understand the functional role of CDK5 in hair color determination, we constructed CDK5-knockdown mice and identified the effect on the mitogen-activated protein kinase (MAPK) pathway in the mouse skin. Quantitative real-time polymerase chain reaction, co-immunoprecipitation, and western blotting were performed to analyze the effects of CDK5-knockdown on the MAPK pathway in mice. The results showed that MAP3K6 was inhibited by phosphorylated CDK5 through its activator CDK7. The decrease in MAP3K6 levels caused down-regulation of MEK1 and ERK expression, leading to the up-regulation of miR-143-3p, which targets MAP3K6 via Dicer. Taken together, our findings indicate that CDK5 functions in regulating the MAPK pathway. Given that MAP3K6 was inhibited in two directions, this mechanism can provide insight into the contributions of the MAPK/ERK pathway to the inhibition of melanin production.

      PubDate: 2017-11-12T01:00:23Z
      DOI: 10.1016/j.acthis.2017.10.009
  • Quercetin protects jejunal mucosa from experimental intestinal ischemia
           reperfusion injury by activation of CD68 positive cells
    • Authors: Kristina Curgali; Stefan Toth; Zuzana Jonecova; Milan Maretta; Theodoros Kalpakidis; Ivana Petriskova; Matus Kusnier; Jan Soltes; Martin Svana; Martin Caprnda; Delian Delev; Luis Rodrigo; Eva Mechirova; Peter Kruzliak
      Abstract: Publication date: Available online 10 November 2017
      Source:Acta Histochemica
      Author(s): Kristina Curgali, Stefan Toth, Zuzana Jonecova, Milan Maretta, Theodoros Kalpakidis, Ivana Petriskova, Matus Kusnier, Jan Soltes, Martin Svana, Martin Caprnda, Delian Delev, Luis Rodrigo, Eva Mechirova, Peter Kruzliak
      The aim of our study was to analyse the possible protective effect of quercetin application during the jejunal ischemia-reperfusion injury (IRI) in rats. Quercetin was administered intraperitoneally 30min before 1h ischemia of superior mesenteric artery with following 24h lasting reperfusion period. The male specific pathogen-free (SPF) Charles River Wistar rats were used. In the group with applied quercetin, the significantly increased (p< 0.001) levels of anti-inflammatory cytokine IL10 were observed both in the blood serum and jejunal tissue. The improvement of the mucosal tissue morphology and proliferating and DNA repairing cell number measured by PCNA activity were recorded by more than 30% higher in the quercetin group. Simultaneously, significant elongation of the intestinal glands (p< 0.001) and increase in the number of CD68-positive cells in the lamina propria mucosae (p< 0.001) in comparison with control group were found. Based on our results, the preventive application of quercetin before induction of jejunal IRI stimulates faster jejunal mucosa restoration and it seems to have immunomodulatory and anti-inflammatory effects as well. CD68-positive macrophages could have crucial role in this process since they work as both growth factor and cytokine producers.

      PubDate: 2017-11-12T01:00:23Z
      DOI: 10.1016/j.acthis.2017.11.001
  • Olive oil polyphenols extracts inhibit inflammatory markers in J774A.1
           murine macrophages and scavenge free radicals
    • Authors: Marwa Abdallah; Stefania Marzocco; Simona Adesso; Mokhtar Zarrouk; Mokhtar Guerfel
      Abstract: Publication date: Available online 8 November 2017
      Source:Acta Histochemica
      Author(s): Marwa Abdallah, Stefania Marzocco, Simona Adesso, Mokhtar Zarrouk, Mokhtar Guerfel
      Here we evaluate the olive oil antiradical and anti-inflammatory potential through its polyphenols extracts and examine the influence of olive maturity on olive oil quality properties, polyphenols composition and biological potentials. Samples have been obtained from minor Tunisian olive cultivars (Chemchali, Fouji and Zarrazi) at different maturity indices. Principal quality properties were evaluated and polyphenols analysis was carried out by Folin Ciocalteu reagent and HPLC-UV-MS. Antiradical activity was examined by DPPH and FRAP scavenging assays while J774A.1 murine macrophages were used to evaluate anti-inflammatory potential by analyzing NO production with Griess reagent method and iNOS and COX-2 expression by cytofluorimetric analysis. Our results revealed that quality characteristics, total phenol content, as well as phenolic compound concentrations were significantly affected by the olive maturity levels. On the other hand, the polyphenols extracts showed an interesting radical scavenging capacity and a potential ability to inhibit inflammatory markers at 90% for NO release and 75% for iNOS expression. Thus, our study establishes that olive oil through its polyphenols extracts has a substantial antiradical and anti-inflammatory potential. Likewise a lot of attention should be attributed to olive ripening level in order to decide the optimum harvesting time.
      Graphical abstract image

      PubDate: 2017-11-12T01:00:23Z
      DOI: 10.1016/j.acthis.2017.10.005
  • Editorial Board ((ofc))
    • Abstract: Publication date: October 2017
      Source:Acta Histochemica, Volume 119, Issue 8

      PubDate: 2017-11-12T01:00:23Z
  • Stemness distinctions between the ectomesenchymal stem cells from neonatal
           and adult mice
    • Authors: Qian Chen; Huangao Zhou; Pingping Hu
      Abstract: Publication date: Available online 27 October 2017
      Source:Acta Histochemica
      Author(s): Qian Chen, Huangao Zhou, Pingping Hu
      Ectomesenchymal stem cells (EMSCs), a type of adult stem cells derived from cranial neural crest, can be non-invasively harvested from respiratory mucosa and play vital roles in therapies based on their stemness. However, whether donor age has any impact on the stemness of EMSCs remains elusive and is essential for EMSCs-based therapies. To address this, we first cultivated EMSCs from neonatal mice aged 1 week and adult mice aged 3 months or 6 months, and then compared their morphology, proliferative capacity, and pluripotency through various induced differentiation assays. The results showed that neonatal EMSCs were fibroblast-like, more regular compared to adult EMSCs; the proliferative capacity of neonatal EMSCs was higher than that of adult EMSCs. More importantly, after neural, adipogenic, chondrogenic, and osteogenic differentiation, neonatal EMSCs differentiated into respective cell types significantly better than adult EMSCs. Notably, EMSCs from mice aged 3 months differentiated into mesodermal lineages better than those from 6 months old mice after induction. Collectively, these results suggest donor ages have significant impact on the EMSCs from respiratory mucosa.

      PubDate: 2017-10-28T22:56:21Z
      DOI: 10.1016/j.acthis.2017.10.008
  • Adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol
           ethers treatment
    • Authors: Anna A. Tyrtyshnaia; Igor V. Manzhulo; Ruslan M. Sultanov; Ekaterina V. Ermolenko
      Abstract: Publication date: Available online 26 October 2017
      Source:Acta Histochemica
      Author(s): Anna A. Tyrtyshnaia, Igor V. Manzhulo, Ruslan M. Sultanov, Ekaterina V. Ermolenko
      Neuropathic pain manifested by a number of sensory symptoms is often accompanied by disorders of higher nervous activity, such as memory impairment, depression, anxiety, anhedonia, etc. This emphasizes the involvement of supraspinal structures including the hippocampus in neuropathic pain pathogenesis. In the present study, we focused on the impact of chronic neuropathic pain on hippocampal neurogenesis and microglial state. In addition, we test the effect of alkyl glycerol ethers on hippocampal neuronal and microglial plasticity as well as behavioral parameters. Neuropathic pain was induced using the model of sciatic nerve chronic constriction injury. We found an impairment of working memory and locomotor activity in animals with neuropathic pain, which was prevented by alkyl glycerol ethers treatment. Sciatic nerve ligation in mice contributed to the decrease in hippocampal neurogenesis intensity. Alkyl glycerol ethers administration significantly reduced this effect. Neuropathic pain-associated neurogenesis reduction was accompanied by an increased percentage of Iba1-labeled area in the CA1 hippocampal region on the 14th and 28th days after surgery. In addition, we observed a decrease in hippocampal pro-inflammatory microglia marker CD86 immunostaining on day 28 after surgery in alkyl glycerol ethers-treated mice with sciatic nerve ligation. These results are consistent with data on pro- and anti-inflammatory cytokines expression in the hippocampus. Alkyl glycerol ethers administration increased IL-10 and decreased IL-1β hippocampal expression in animals with neuropathic pain. Taken together, these data suggest that neuropathic pain-behavior in rodents is accompanied by changes in microglia polarization, thereby contributing to neurogenesis impairment and cognitive disturbances. Alkyl glycerol ethers prevented M1 microglial activation, contributing to the maintenance of normal neurogenesis levels within the hippocampus and normalizing working memory.
      Graphical abstract image

      PubDate: 2017-10-28T22:56:21Z
      DOI: 10.1016/j.acthis.2017.10.007
  • Distribution of mesotocin-immunoreactive neurons in the brain of the male
           native Thai chicken
    • Authors: Boonyarit Kamkrathok; Tom E. Porter; Mohamed E. El Halawani; Yupaporn Chaiseha
      Abstract: Publication date: Available online 19 October 2017
      Source:Acta Histochemica
      Author(s): Boonyarit Kamkrathok, Tom E. Porter, Mohamed E. El Halawani, Yupaporn Chaiseha
      Mesotocin (MT), a homolog of oxytocin (OT) in mammals, is a nonapeptide neurohypophysial hormone that is mainly synthesized in specific neuronal groups within the hypothalamus and released from the posterior pituitary gland in amphibian, reptilian, and avian species. MT is associated with the neuroendocrine regulation of reproductive cycle and maternal behaviors in female native Thai chickens. Male birds exhibit parental behaviors as well. However, there are limited data regarding the role(s) of the MTergic system in males. Thus, the objective of this study was to elucidate the localization of the MT neuronal groups in the brain of male native Thai chickens. The distributions of MT-immunoreactive (-ir) neurons and fibers in the brain were studied utilizing immunohistochemistry technique. The results revealed that MT-ir neurons and fibers were distributed throughout the brain and extensively in the diencephalon. MT-ir neurons and fibers were predominantly located within the nucleus supraopticus, pars ventralis (SOv), nucleus preopticus medialis (POM), nucleus ventrolateralis thalami (VLT), nucleus paraventricularis magnocellularis (PVN), and regio lateralis hypothalami (LHy), suggesting that MT neurons in these nuclei might be involved in the reproductive activities and/or parental behavior in the male chickens. In addition, the numbers of MT-ir neurons within the SOv and POM were significantly higher than those of the VLT, PVN, and LHy. More importantly, the number of MT-ir neurons in the SOv was high in the male brain when compared with the female brain, indicating that the MTergic system in the SOv might play a significant role in male reproductive activities in this equatorial species.

      PubDate: 2017-10-21T21:53:25Z
      DOI: 10.1016/j.acthis.2017.10.004
  • Desmin expression profile in reactive astrocytes in the 3-nitropropionic
           acid–lesioned striatum of rat: Characterization and comparison with
           glial fibrillary acidic protein and nestin
    • Authors: Jeong-Heon Choi; Tae-Ryong Riew; Hong Lim Kim; Xuyan Jin; Mun-Yong Lee
      Abstract: Publication date: Available online 18 October 2017
      Source:Acta Histochemica
      Author(s): Jeong-Heon Choi, Tae-Ryong Riew, Hong Lim Kim, Xuyan Jin, Mun-Yong Lee
      Desmin, a muscle-specific, type-III intermediate-filament protein, is reportedly expressed in astrocytes in the central nervous system. These cells become reactive astrocytes in response to brain injuries. To elucidate whether desmin is involved in this process, we examined the spatiotemporal expression profiles of desmin and their relationship with two astroglial intermediate filaments, glial fibrillary acidic protein (GFAP) and nestin, in the striatum of rats treated with the mitochondrial toxin 3-nitropropionic acid (3-NP). Weak, constitutive immunoreactivity for desmin was observed in astrocytes generally, and in reactive astrocytes in the peri-lesional area, its expression increased in parallel with that of GFAP over 3 d post-lesion and was maintained until at least day 28. Desmin, GFAP, and nestin showed characteristic time-dependent expression patterns in reactive astrocytes forming the astroglial scar; delayed and long-lasting induction of desmin and GFAP, and rapid but transient induction of nestin. In the lesion core, desmin was expressed in two categories of perivascular cells: nestin-negative and nestin-positive. These findings show that desmin, together with GFAP and nestin, is a dynamic component of intermediate filaments in activated astroglia, which may account for the dynamic structural changes seen in these cells in response to brain injuries.

      PubDate: 2017-10-21T21:53:25Z
      DOI: 10.1016/j.acthis.2017.10.003
  • Multipotency of skeletal muscle stem cells on their native substrate and
           the expression of Connexin 43 during adoption of adipogenic and osteogenic
    • Authors: Mohamed I. Elashry; Manuela Heimann; Sabine Wenisch; Ketan Patel; Stefan Arnhold
      Abstract: Publication date: Available online 14 October 2017
      Source:Acta Histochemica
      Author(s): Mohamed I. Elashry, Manuela Heimann, Sabine Wenisch, Ketan Patel, Stefan Arnhold
      Muscle regeneration is performed by resident muscle stem cells called satellite cells (SC). However they are multipotent, being able to adopt adipogenic and osteogenic fate under the correct stimuli. Since SC behavior can be regulated by the extra-cellular matrix, we examined the robustness of the myogenic programme of SC on their native substrate-the surface of a myofiber. We show that the native substrate supports myogenic differentiation judged by the expression of members of the Myogenic Determination Factor (MRF) family. However SC even on their native substrate can be induced into adopting adipogenic or osteogenic fate. Furthermore conditions that support adipose or bone formation inhibit the proliferation of SC progeny as well as their migration. We show that Connexin43 (Cx43), a gap junction complex protein, is only expressed by activated and not quiescent SC. Furthermore, it is not expressed by SC that are in the process of changing their fate. Lastly we show that intact adult mouse muscle contains numerous cells expressing Cx43 and that the density of these cells seems to be related to capillary density. We suggest the Cx43 expression is localized to angioblasts and is more prominent in oxidative slow muscle compared to glycolytic fast muscle.

      PubDate: 2017-10-21T21:53:25Z
      DOI: 10.1016/j.acthis.2017.10.002
  • Glycan diversity in the vomeronasal organ of the Korean roe deer,
           Capreolus pygargus: A lectin histochemical study
    • Authors: Taekyun Shin; Jeongtae Kim; Yuna Choi; Meejung Ahn
      Abstract: Publication date: Available online 10 October 2017
      Source:Acta Histochemica
      Author(s): Taekyun Shin, Jeongtae Kim, Yuna Choi, Meejung Ahn
      Glycans in the epithelium play an important role in cell-to-cell communication and adhesion. No detailed evaluation of glycoconjugates in the vomeronasal organs (VNO) of the roe deer has been published previously. The aim of this study was to characterize glycan epitopes in the vomeronasal sensory epithelium (VSE) and non-sensory epithelium (VNSE) using lectin histochemistry. Glycan epitopes identified by lectin histochemistry were grouped as follows: N‐acetylglucosamine (s-WGA, WGA, BSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose (RCA120, BSL-I, Jacalin, PNA, ECL), N-acetylgalactosamine (VVA, DBA, SBA, and SJA), fucose (UEA-I) and complex type N-glycan (PHA-E and PHA-L) groups. The free border of the VSE was positive for all 21 lectins, and 18 of the lectins (excluding DBA, SJA, and PHA-L) showed weak and/or moderate staining in the receptor cells. The supporting cells were weakly positive for 19 lectins (excluding PNA and SJA). Moreover, 17 lectins (excluding BSL-II, Jacalin, PNA, and SJA) were expressed in the basal cells. In the VNSE of roe deer, the free border showed staining for all 21 lectins examined. The ciliated cells were positive for 16 lectins (excluding BSL-II, DSL, PNA, VVA, and SJA). Furthermore, 15 lectins (excluding DSL, LEL, ECL, UEA-I, PHA-E, and PHA-L) were expressed in goblet cells. Twenty lectins (excluding SJA) were expressed in the acini of the vomeronasal glands. Collectively, both VSE and VNSE were rich in N-acetylglucosamine, mannose, galactose, N‐acetylgalactosamine, fucose, and complex-type N-glycans, although the different cell types of the VSE and VNSE expressed different glycoconjugates of varying intensities, suggesting that these carbohydrate residues may be involved in odor perception as well as cell-to-cell communication in the VNO.

      PubDate: 2017-10-13T21:13:05Z
      DOI: 10.1016/j.acthis.2017.10.001
  • Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea:
           Axiidea): a morphofunctional and histochemical study
    • Authors: Souza Adriane; Braga Laura Erika Nunes
      Abstract: Publication date: Available online 6 October 2017
      Source:Acta Histochemica
      Author(s): Tugstênio L. Souza, Adriane A. Braga, Laura S. López-Greco, Erika T. Nunes
      Callichirus major, popularly known as ghost shrimp, is a species of great importance in the fishing industry, because of its use as live bait. This study aimed to describe the different stages of the developing ovaries in C. major. Shrimps were collected along the Corujão beach, Piuma, Brazil (20°50'41.6“S 40°44'15.7“W), and the gonads were dissected for histological and histochemical analysis. The ovary consists of two elongated filaments covered by a connective tissue that divides the organ into lobules, where somatic and germ cells are found. It was possible to classify five types of germ cells: Oogonia (Oog), previtellogenic oocyte (Oc1), early vitellogenic oocyte (Oc2), late vitellogenic oocyte (Oc3) and mature oocyte (Oc4) based on their vitellogenic stage, cytoplasmic, nuclear and morphometric characteristics. The histochemical analysis demonstrated an intense reaction for proteins and polysaccharides in peripheral cytoplasm of Oc3 comparing to others cell types. According to size, volume, color intensity and distribution of oocyte types the gonads were classified into: immature, developing, developed and spent, being in females at this last stage, observed empty follicles and oocytes in reabsorption process. During oogenesis was observed a gradual increase in cytoplasmic acidophilia due to accumulation of yolk granules and the intense histochemical reaction in periphery of Oc3, which indicate the beginning of an extravitellogenic source of nutrients. Based on the microscopic analysis of the vitellogenesis, shrimp C. major showed the initial short phase of oocyte growth following with a fast vitellogenic cycle.

      PubDate: 2017-10-08T20:42:07Z
  • Editorial Board ((ofc))
    • Abstract: Publication date: September 2017
      Source:Acta Histochemica, Volume 119, Issue 7

      PubDate: 2017-09-30T18:44:05Z
  • Progesterone down-regulates SLIT/ROBO expression in mouse corpus luteum
    • Authors: Xuejing Zhang; Meiyan Mi; Weili Hao; Qiongying Fan; Bulang Gao
      Abstract: Publication date: Available online 23 September 2017
      Source:Acta Histochemica
      Author(s): Xuejing Zhang, Meiyan Mi, Weili Hao, Qiongying Fan, Bulang Gao
      Background Progesterone produced by the corpus luteum (CL) is essential for preparation, implantation and maintenance of gestation. Furthermore, progesterone plays a protective role against luteolysis in rodents. It has been reported that Slit/Robo family members expressed in the CL and involved in prostaglandin F2α (PGF2α) induced luteolysis. However, the interactions between progesterone and Slits/Robos in CL are not clear. This study was designed to examine whether or not luteolysis is regulated by the interaction of progesterone and Slits/Robos in mouse CL. Methods In the current study, we used Real-time PCR to identify the effect of progesterone on Slit2/Robo1 expression in cultured luteal cells in vitro, and the exogenous progesterone injection on mouse luteolysis and Slit/Robo expression in vivo was studied via Real-time PCR and Western bolt. Results Our in vitro experiment revealed that 1μM progesterone significantly decreased Slit2/Robo1 mRNA levels at 6h, 12h and 24h. Our in vivo experiment showed that the mRNA and protein levels of Slit2 and Robo1 decreased significantly 7days after progesterone supplement. Conclusion These findings indicate that progesterone maintains CL function and resists luteolysis possibly through down-regulating Slit/Robo signaling pathway in the CL.

      PubDate: 2017-09-23T17:34:09Z
      DOI: 10.1016/j.acthis.2017.09.006
  • Melatonin modulates monochromatic light-induced melatonin receptor
           expression in the hypothalamus of chicks
    • Authors: Liwei Zhang; Funing Chen; Jing Cao; Yulan Dong; Zixu Wang; Yaoxing Chen
      Abstract: Publication date: Available online 22 September 2017
      Source:Acta Histochemica
      Author(s): Liwei Zhang, Funing Chen, Jing Cao, Yulan Dong, Zixu Wang, Yaoxing Chen
      To study the mechanism of the effect of monochromatic light on physiological function in chicken, a total of 192 newly hatched chicks were randomly divided into intact, sham-operated and pinealectomy groups then exposed to white light (WL), red light (RL), green light (GL) and blue light (BL) using a light-emitting diode (LED) system for two weeks. At P14, the hypothalami were immediately collected for immunohistochemical staining of melatonin receptor subtypes (Mel1a and Mel1b) and detection of Mel1a and Mel1b expressions using RT-PCR and western blot. Immunohistochemical staining of the hypothalamus showed that the Mel1a-ir cells were distributed in the preoptic area (POA), nucleus preopticus periventricularis (POP) and suprachiasmatic nuclei (SCN), and the Mel1b-ir cells were presented in the POA and SCN. Analysis of RT-PCR and western blot showed that the mRNA and protein levels of Mel1a and Mel1b in the hypothalamus of chick exposed to GL were increased by 10.7–29.3%, 9.18–35.9% and 8.97–27.3% compared to those in the chicks exposed to WL (P =0.029–0.002), RL (P =0.027–0.001) and BL (P =0.038–0.007) in the intact group, respectively. After pinealectomy, however, these parameters decreased and there were no significant differences among the WL, RL, GL and BL groups. These findings suggested that melatonin plays a critical role in GL illumination-enhanced Mel1a and Mel1b expressions in the hypothalamus of chicks.

      PubDate: 2017-09-23T17:34:09Z
      DOI: 10.1016/j.acthis.2017.09.005
  • Histological and immunohistochemical study of cardiac telocytes in a rat
           model of isoproterenol-induced myocardial infarction with a reference to
           the effect of grape seed extract
    • Authors: Mai Salah Nour; Nahla Reda Sarhan; Shireen A. Mazroa; Salwa A. Gawish
      Abstract: Publication date: Available online 21 September 2017
      Source:Acta Histochemica
      Author(s): Mai Salah Nour, Nahla Reda Sarhan, Shireen A. Mazroa, Salwa A. Gawish
      Introduction Cardiac telocytes (TCs) represent a unique type of cells that make a supportive network for stem cells that contribute in cardiac renewal, but their role during myocardial infarction (MI) is not clear. Grape seed extract (GSE) is a powerful natural antioxidant. Aim of the work Quantitative study of cardiac TCs in a rat model of Isoproterenol (ISO)-induced MI, and to evaluate the effect of GSE on TCs and MI progression. Materials and methods Seventy adult male albino rats were assigned into 4 groups; group I; control rats, group II received GSE (100mg/kg/day) dissolved in distilled water orally, group III received 2 intra-peritoneal injections of 85mg/kg ISO dissolved in saline on 14th and 15th day to induce MI, and group IV received GSE and ISO. Myocardium was obtained 1 and 14days after ISO i.e. on day 16 and day 30 respectively. Tissue was prepared for histological and immunohistochemical study of CD117 and CD34 as two markers for TCs. CD34 was used also as a marker for angiogenesis. Results Group III showed focal areas of myocardial infarction 1day and 14days after ISO. Degenerated cardiomyocytes showed loss of striation and hypereosinophilic vacuolated cytoplasm with condensed nuclei. Mononuclear cell infiltration and a significantly increased percentage area of fibrosis 14days after ISO were observed. CD117 and CD34 positive TCs were hardly detected 1day after ISO. Their number slightly increased 14days after ISO with insignificant difference to control. There was also a significant increase in the number of CD34 positive blood vessels 14days after ISO. Group IV showed much better histological picture with a significant decrease in the percentage area of fibrosis and a significant increase in the number of CD117 and CD34 positive TCs and the number of CD34 positive blood vessels as compared to group III. Conclusion Telocytes were significantly decreased in MI. GSE reduced ISO-induced histological changes and increased the number of TCs that improved angiogenesis.

      PubDate: 2017-09-23T17:34:09Z
      DOI: 10.1016/j.acthis.2017.09.007
  • Different combinations of maternal and postnatal diet are reflected in
           changes of hepatic parenchyma and hepatic TNF-alpha expression in male rat
    • Authors: Željka Perić Kačarević; Anđela Grgić; Darija Šnajder; Nikola Bijelić; Tatjana Belovari; Olga Cvijanović; Valerija Blažičević; Radivoje Radić
      Abstract: Publication date: Available online 18 September 2017
      Source:Acta Histochemica
      Author(s): Željka Perić Kačarević, Anđela Grgić, Darija Šnajder, Nikola Bijelić, Tatjana Belovari, Olga Cvijanović, Valerija Blažičević, Radivoje Radić
      Obesity is related to increased TNF-alpha production in different tissues. TNF-alpha is connected to mitochondrial dysfunction in the liver and also development of fatty infiltration of the liver. Also, postnatal change from normal to high-fat diet causes a significant increase in TNF-alpha serum levels. The aim of this research was to determine how maternal diet and switching male offspring to a different dietary regime after lactation influences rat liver. Ten female Sprague Dawley rats at nine weeks of age were randomly divided in two groups and fed either standard laboratory chow or high-fat diet during six weeks, and then mated with the same male subject. After birth and lactation male offspring from both groups were further divided into four subgroups depending on their subsequent diet. At 22 weeks of age, the animals were weighted, sacrificed and major organs were collected and weighted. Immunohistochemistry for TNF-alpha was performed on liver, and liver samples were analyzed for pathohistological changes. The group in which mothers were fed standard chow and offspring high-fat diet had the most pronounced changes: heaviest liver, poorest histopathological findings and strongest TNF-alpha immunohistochemical staining of liver parenchyma. High-fat diet during pregnancy and lactation and switching to high-fat diet postnatally affects liver weight, histological structure and TNF-alpha expression in male offspring.

      PubDate: 2017-09-23T17:34:09Z
      DOI: 10.1016/j.acthis.2017.09.003
  • Effects of nest-deprivation on hypothalamic mesotocin in incubating native
           Thai hens (Gallus domesticus)
    • Authors: Panpradap Sinpru; Tom E. Porter; Mohamed E. El Halawani; Yupaporn Chaiseha
      Abstract: Publication date: Available online 15 September 2017
      Source:Acta Histochemica
      Author(s): Panpradap Sinpru, Tom E. Porter, Mohamed E. El Halawani, Yupaporn Chaiseha
      Avian mesotocin (MT) is homologous to oxytocin in mammals. Native Thai chickens (Gallus domesticus) strongly express maternal behaviors including incubation and rearing. However, the role of MT during incubation behavior has never been studied. The objective of this study was to determine the physiological function(s) of the MTergic system in incubation behavior in native Thai chickens. The brains were collected from incubating (INC) and nest-deprived (ND) hens at different time points (days 3, 6, 8, 10, 14, 18, and 21; n=6). Immunohistochemistry technique was used to compare the numbers of MT-immunoreactive (-ir) neurons between the INC and ND hens within the Nucleus supraopticus, pars ventralis (SOv), Nucleus preopticus medialis (POM), and Nucleus paraventricularis magnocellularis (PVN). The results revealed that the numbers of MT-ir neurons within the SOv, POM, and PVN remained high during the incubating stage. The number of MT-ir neurons in the SOv was lower than that of the POM and PVN. Disruption of incubation behavior by nest deprivation caused the numbers of MT-ir neurons within the SOv, POM, and PVN to decrease throughout the observation periods. For the first time, this study demonstrates that the MTergic system within the SOv, POM, and PVN may be involved with incubation behavior. In addition, these results further suggest that the MTergic neurons in these nuclei are not only regulated by rearing behavior but also might have a role in the initiation and maintenance of incubation behavior in this tropical species.

      PubDate: 2017-09-17T15:42:22Z
      DOI: 10.1016/j.acthis.2017.09.002
  • The presence of abalone egg-laying hormone-like peptide in the central
           nervous system and ovary of the Spotted Babylon, Babylonia areolata
    • Authors: Jirawat Saetan; Piyakorn Boonyoung; Uraporn Vongvatcharanon; Thanapong Kruangkum; Kanjana Khornchatri; Pinij Thaweethamsewee; Prasert Sobhon; Prapee Sretarugsa
      Abstract: Publication date: Available online 14 September 2017
      Source:Acta Histochemica
      Author(s): Jirawat Saetan, Piyakorn Boonyoung, Uraporn Vongvatcharanon, Thanapong Kruangkum, Kanjana Khornchatri, Pinij Thaweethamsewee, Prasert Sobhon, Prapee Sretarugsa
      Recently, the neuronal classification of the ivory shell Spotted Babylon, Babylonia areolata, was readily demonstrated. Regarding its importance as marine economic molluscan species, the attempt to understand the neuroendocrine regulation is necessary. This study firstly demonstrated the neurosecretory cells as well as the existence and distribution of the egg-laying hormone (ELH)-like peptide in the central nervous system (CNS) and ovary of the B. areolata. The neurosecretory cell was characterized by the cytoplasmic purple dot-like structure as stained by the Gomori’s paraldehyde fuchsin. Using the anti-abalone (a) ELH, we detected the aELH-like-peptide in neurons (Nr) and neurosecretory cells (Ns) of all ganglia including the cerebral, pleural, parietal, pedal and buccal ganglia. The aELH-like peptide was also present in the neuropil of each. It was noted that not all Ns presented the aELH-like peptide. In the ovary, the aELH-like peptide was slightly detected in early developing oocytes and strongly detected in late developing oocytes and follicular cells. This study firstly reported the evidence of ELH-like peptide in the CNS and ovary of the B. areolata. The molecular cloning as well as to investigate the function of ELH in this species is needed as it will be beneficial for future applications in aquaculture.

      PubDate: 2017-09-17T15:42:22Z
      DOI: 10.1016/j.acthis.2017.08.006
  • Seasonal expression of luteinizing hormone receptor and follicle
           stimulating hormone receptor in testes of the wild ground squirrels
           (Citellus dauricus Brandt)
    • Authors: Junjie Wang; Ying Wang; Manyu Zhu; Fengwei Zhang; Xia Sheng; Haolin Zhang; Yingying Han; Zhengrong Yuan; Qiang Weng
      Abstract: Publication date: Available online 12 September 2017
      Source:Acta Histochemica
      Author(s): Junjie Wang, Ying Wang, Manyu Zhu, Fengwei Zhang, Xia Sheng, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng
      The objective of this study was to evaluate whether luteinizing hormone (LH), follicle stimulating hormone (FSH) and their receptors luteinizing hormone receptor (LHR) and follicle stimulating hormone receptor (FSHR) play roles in the seasonal spermatogenesis of the wild ground squirrels. To that end, we characterized the testicular immunolocalization of LHR and FSHR, their expression on both mRNA and protein levels, as well as serum concentrations of LH and FSH in male wild ground squirrels throughout the annual reproductive cycle. Histologically, all types of spermatogenic cells including mature spermatozoa were identified in the breeding season (April), while spermatogonia and primary spermatocytes were observed in the non-breeding season (June), and spermatogonia, primary spermatocytes and secondary spermatocytes were found in pre-hibernation (September). LHR was present in Leydig cells during the whole periods with more intense staining in the breeding season; Stronger immunostaining of FSHR was observed in Sertoli cells during the breeding season compared to the non-breeding season and pre-hibernation. Consistently, the mRNA and protein levels of LHR and FSHR were higher in testes of the breeding season, and then decreased to a relatively lower level in the non-breeding season and pre-hibernation. Meanwhile, serum LH and FSH concentrations were significantly higher in the breeding season than those in the non-breeding season and pre-hibernation. These results suggested that gonadotropins and its receptors, LHR and FSHR may be involved in the regulation of seasonal changes in testicular functions of the wild ground squirrels.

      PubDate: 2017-09-17T15:42:22Z
      DOI: 10.1016/j.acthis.2017.09.004
  • Immunohistochemical study of arginases 1 and 2 in the olfactory bulbs of
           the Korean roe deer, Capreolus pygargus
    • Authors: Jeongtae Kim; Meejung Ahn; Yuna Choi; Ji-yeon Hyeon; Taekyun Shin
      Abstract: Publication date: Available online 5 September 2017
      Source:Acta Histochemica
      Author(s): Jeongtae Kim, Meejung Ahn, Yuna Choi, Ji-yeon Hyeon, Taekyun Shin
      Arginases are enzymes of the urea cycle that catalyze the hydrolysis of l-arginine to ornithine and urea. The enzymes are core components of the arginine–ornithine–glutamate–γ-amino butyric acid pathway of the central nervous system. In the present study, we immunohistochemically determined the localization of arginase 1 and 2 in the olfactory bulb (OB) of the roe dear (Capreolus pygargus). Reverse transcription PCR revealed that the mRNAs encoding both arginase 1 and 2 were expressed in the OB. Arginase 1 was localized to olfactory nerve axons, calcitonin gene-related peptide-positive mitral/tufted cells (excitatory neurons), and glutamate acid decarboxylase 65/67-immunopositive periglomerular cells of the main olfactory bulb. The arginase 2 immunoreactivities in the OB tissues were similar to those of arginase 1. Furthermore, both arginases were detected in the accessory olfactory bulb. These findings suggest that both arginase 1 and 2 are potentially associated with excitatory and inhibitory neurotransmitter activities in animal OBs, including those of the roe deer.

      PubDate: 2017-09-06T15:34:21Z
      DOI: 10.1016/j.acthis.2017.08.005
  • Overexpression of suppressors of cytokine signaling 1 regulate the
           proliferation and differentiation of rat-derived neural stem cells
    • Authors: Meng Cui; Xin-Long Ma; Jie Sun; Jin-Quan He; Lin Shen; Fang-Guo Li
      Abstract: Publication date: Available online 1 September 2017
      Source:Acta Histochemica
      Author(s): Meng Cui, Xin-Long Ma, Jie Sun, Jin-Quan He, Lin Shen, Fang-Guo Li
      Neural stem cells are a reliable resource in various neural tissue repair and neurodegenerative diseases. Increasing evidence has demonstrated that Suppressor of cytokine signaling proteins (SOCS) was involved in the nervous system development. The universality and diversity of SOCS also suggested their important roles in neurogenesis and nerve regeneration. In this study, we employed a lentiviral vector to investigate the impacts of overexpression SOCS1 on the proliferation and differentiation of rat-derived NSCs. Cells infected with LV-EGFP-SOCS1 showed a prominent increased cell number, diameter, and metabolic activity compared with other groups. Immunofluorescence analysis revealed the proportion of cells positive for microtubule associated protein-2 (MAP2) or myelin basic protein (MBP) was significantly increased in LV-EGFP-SOCS1 group while the proportion of glial fibrillary acidic protein (GFAP)-positive cells in LV-EGFP-SOCS1 group was significantly decreased compare to LV-EGFP and PBS group. Moreover, Western blot results were consistent with immunofluorescence results which indicated that overexpression of SOCS1 could promote neuronal and oligodendrocyte differentiations of NSCs but inhibit astrocyte differentiation of NSCs. In conclusion, our findings provided evidence that SOCS1 could promote the proliferation of NSCs and affect the differentiation of NSCs, providing a potential target for NSCs transplantation strategies.

      PubDate: 2017-09-06T15:34:21Z
      DOI: 10.1016/j.acthis.2017.08.003
  • Bioinformatics analyses of pathways and gene predictions in IL-1α and
           IL-1β knockout mice with spinal cord injury
    • Authors: Zhuangchen Zhu; Defeng Wang; Wei Jiao; Guang Chen; Yan Cao; Qingfu Zhang; Junqin Wang
      Abstract: Publication date: Available online 26 August 2017
      Source:Acta Histochemica
      Author(s): Zhuangchen Zhu, Defeng Wang, Wei Jiao, Guang Chen, Yan Cao, Qingfu Zhang, Junqin Wang
      Purpose This study aimed to explore the potential genes and pathways regulated in spinal cord injury (SCI) model mice with IL-1α and IL-1β knockout (KO). Methods Gene expression profile GSE70302, which includes data from injured spinal cord of 4 IL-1α-KO mice, 4 IL-1β-KO mice and 4 C57BL with 6 mice as controls was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) of the IL-1α-KO or IL-1β-KO vs. control, and IL-1α-KO vs. IL-1β-KO groups were screened, followed by function enrichment and protein–protein interaction (PPI) analyses. Finally, miRNAs associated with SCI that may target the DEGs were predicted. Results A total of 579 and 992 DEGs were selected from the IL-1α-KO vs. control group and the IL-1β-KO vs. control group, respectively, and 208 genes common between the 2 comparison groups were identified. Additionally, 526 DEGs were identified from the IL-1α-KO vs. IL-1β-KO groups. These DEGs were significantly enriched in functions and pathways associated with ion transport, neuron apoptotic processes and inflammatory responses. The common genes were enriched in the pathways for cytokine–cytokine receptor interaction. DEGs of IL-1α-KO vs. IL-1β-KO were significantly enriched in the immune system, hematopoietic cell lineage and PI3K-Akt signalling pathway-associated biological processes and pathways. The PPI network consisted of 76 nodes, such as Saa2, Kcna1, Scn8a, Ccl5, Ccl28 and Pink1. A total of 94 miRNAs, including mir-17-5P and mir-30a-5p were predicted that could target the DEGs. Conclusion IL-1α and IL-1β may play important roles in SCI by regulating ion transport, inflammation and neuron apoptotic processes and their associated genes or miRNAs. Compared with IL-1β-KO, IL-1α-KO may improve the outcome of SCI via the alteration of hematopoietic cell lineage and PI3K-Akt signalling pathways.

      PubDate: 2017-08-27T15:27:16Z
      DOI: 10.1016/j.acthis.2017.07.007
  • Mesenchymal cells are required for epithelial duct cell-to-beta cell
           maturation and function in an injured adult pancreas in the rat
    • Authors: Juziel Kampando Manda; Benedict John Page; Venant Tchokonte-Nana
      Abstract: Publication date: Available online 26 August 2017
      Source:Acta Histochemica
      Author(s): Juziel Kampando Manda, Benedict John Page, Venant Tchokonte-Nana
      The islet, the endocrine portion of the pancreas − develops from an invagination of the pancreatic duct epithelial cells (PDECs) into the surrounding tissue. The contact of the PDECs with mesenchymal cells (MSCs) may be an essential drive for endocrine cell fate. During pancreatic development, cells that express Neurogenin-3 (Ngn3) biomarker are precursors of insulin- producing beta cells. These precursors have been reported in the neogenesis of islets from adult tissues following the surgical ligation of the main pancreatic duct (PDL). But the capacity of these precursors to induce the appropriate signals to complete the entire neogenesis program has been questioned. We studied the fate of co-culture of PDECs and MSCs from the ligated adult pancreas and established the exact location of adult stem- or progenitor-like cells that give rise to beta cells. PDECs were cultured in direct contact with or without MSCs in serum-containing culture media. The cytomorphology of the cells in co-cultures was determined and the immunocytochemical study of the cells was carried out using anti-Ngn3, anti-insulin and anti-cytokeratin-7 (CK7) antibodies. Both the PDEC/MSC- and PDEC/MSC+ cultures showed out- pocketing from duct epithelium by the end of the second week, which are distinct as cell clusters only in PDEC/MSC+ cells later in week four, exhibiting numerous branching ducts. Co-expression of Ngn3 with insulin was observed in both cultures from the second week. However, characterizations of these Ngn3+ cells in the PDEC/MSC+ culture revealed that these cells also co-expressed a CK7 biomarker. This study provides new evidence of the ductal epithelial nature of beta cells in injured adult pancreata; and that the mesenchymal stromal cells are required to sustain Ngn3 expression for beta cell maturation and function.

      PubDate: 2017-08-27T15:27:16Z
      DOI: 10.1016/j.acthis.2017.08.004
  • Gonadal morphogenesis and establishment of the germline in the
           phyllostomid bat Sturnira lilium
    • Authors: Tania Janeth Porras-Gómez; Adriana Martínez-Juárez; Norma Moreno-Mendoza
      Abstract: Publication date: Available online 18 August 2017
      Source:Acta Histochemica
      Author(s): Tania Janeth Porras-Gómez, Adriana Martínez-Juárez, Norma Moreno-Mendoza
      In vertebrates such as the mouse and the human, primordial germ cells (PGCs) arise at the base of the allantois and are carried to the epithelium of the posterior intestine, to later migrate to the primordial gonads. In the case of bats, almost nothing is known about this process. To clarify the dynamics of PGCs during gonadal morphogenesis in the phyllostomid bat Sturnira lilium, the proteins for the Ddx4, Sox9 and Mis genes were detected in the gonads of embryos at different stages of development. We identified 15 stages (St) of embryonic development in Sturnira lilium. We found that the formation of the genital ridge and the establishment of the undifferentiated gonad take place between stages 11 and 14. The onset of morphological differentiation in the gonad is first detected in the male gonads at St17. The first PGCs in meiosis are detected in the ovary at St19, whereas in the testicles, the PGCs were in mitotic arrest. Structural changes leading to testicular and ovarian development in Sturnira lilium are observed to be similar to those described for the mouse; however, differences will be established concerning the time taken for these processes to occur.

      PubDate: 2017-08-27T15:27:16Z
      DOI: 10.1016/j.acthis.2017.08.002
  • Editorial Board ((ofc))
    • Abstract: Publication date: July 2017
      Source:Acta Histochemica, Volume 119, Issue 6

      PubDate: 2017-08-27T15:27:16Z
  • Expression of glucose transporter 8 (GLUT8) in spermatogenesis of adult
           boar testes
    • Authors: Huaming Xi; Zhen Zhang; Rui Ji; Hui Shen; Xiaorui Fan; Qinghong Li; Xiyun Bai; Zhixue Cheng; Junping He
      Abstract: Publication date: Available online 4 August 2017
      Source:Acta Histochemica
      Author(s): Huaming Xi, Zhen Zhang, Rui Ji, Hui Shen, Xiaorui Fan, Qinghong Li, Xiyun Bai, Zhixue Cheng, Junping He
      The present study was designed to investigate the glucose transporter 8 (GLUT8) expression and localization in adult boar testis. Localization and expression of GLUT8 were conducted with Western Blotting, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods GLUT8 protein and mRNA were expressed in the boar testes. The results of Western Blotting analysis showed specificity of the antibody for protein of boar testes. The immunohistochemistry results showed that GLUT8 protein mainly localized in spermatocytes, round spermatids and elongated spermatids of the seminiferous tubules in the adult boar testes. And the GLUT8 expression persists during eight stages of boar spermatogenesis. GLUT8 may mainly provide glucose for the later stage of germ cell differentiation in adluminal compartment in adult boar testes. These results suggested that GLUT8 is important for the spermatogenesis in the adult boar testes.

      PubDate: 2017-08-06T12:25:11Z
      DOI: 10.1016/j.acthis.2017.07.004
  • The Feulgen reaction: A brief review and new perspectives
    • Authors: Maria Luiza S. Mello; Benedicto de Campos Vidal
      Abstract: Publication date: Available online 21 July 2017
      Source:Acta Histochemica
      Author(s): Maria Luiza S. Mello, Benedicto de Campos Vidal
      The Feulgen reaction has been proposed by Robert Feulgen and Heinrich Rossenbeck for the identification of DNA nearly a hundred years ago. Since then, many other applications of this cytochemical/topochemical procedure at qualitative and quantitative level have been proposed in relation to DNA and its role in chromatin in human, animal and plant cells. In this article, we briefly review some fundamental aspects of the Feulgen reaction and current applications of such a method in studies of altered chromatin texture, including its association with or preceding changes in transcriptional activities and effect on epigenetic marks. Further perspectives on the use of the Feulgen reaction will depend of the proposal of innovative biological questions in which its reveals appropriate.

      PubDate: 2017-07-28T04:01:54Z
      DOI: 10.1016/j.acthis.2017.07.002
  • Assessment of bone repair in critical-size defect in the calvarium of rats
           after the implantation of tricalcium phosphate beta (β-TCP)
    • Authors: Leonardo de Freitas Silva; Erik Neiva Ribeiro de Carvalho Reis; Tânia Aparecida Barbara; João Paulo Bonardi; Idelmo Rangel Garcia; Paulo Sérgio Perri de Carvalho; Daniela Ponzoni
      Abstract: Publication date: Available online 18 July 2017
      Source:Acta Histochemica
      Author(s): Leonardo de Freitas Silva, Erik Neiva Ribeiro de Carvalho Reis, Tânia Aparecida Barbara, João Paulo Bonardi, Idelmo Rangel Garcia, Paulo Sérgio Perri de Carvalho, Daniela Ponzoni
      Objectives Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. Materials and Methods Critical-size defects of 7mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty. The animals were euthanized at two different time points (30 and 60days post-operation). All defects were recovered with a absorbable membrane of bovine cortical bone. Histological, histometric, and immunohistochemical (osteocalcin) assessments were carried out at 30 and 60days post-operation. Results At 30days post-operation, all groups showed areas of bone formation, predominantly when autogenous grafts were used. However, there were no statistically significant differences between the treatment groups (p>0.05). After 60days, there were similarities in the bone formation patterns between the β-TCP (26.32±) and Bio-Oss (17.35±) groups (p=0.549). In terms of the immunohistochemical assessment of osteocalcin, the clot group showed light to moderate staining at 30 and 60days. The autogenous group showed moderate staining at 30days and moderate to intense staining after 60days. The Bio-Oss group showed light to moderate staining after 30days and intense staining at 60days. The β-TCP group showed moderate staining at 30 and 60days post-operation. Conclusion β-TCP is a good osteoconductive material with similar effects to those of inorganic bovine bone graft and is suitable for utilization in the repair of bone defects.

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.07.003
  • FoxP3 and TLR2 in co-expression in oral cancer
    • Authors: H.M. Hussaini; V.P.B. Parachuru; G.J. Seymour; A.M. Rich
      Abstract: Publication date: Available online 17 July 2017
      Source:Acta Histochemica
      Author(s): H.M. Hussaini, V.P.B. Parachuru, G.J. Seymour, A.M. Rich

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.006
  • Is toll-like receptor-2 really inhibiting FoxP3+ TLR2+ cells and enhancing
           the anti-tumour response in oral squamous cell carcinoma'
    • Authors: Xi Zeng; Mao Li; Guang-dong Liao; Ming-Rong Xi
      Abstract: Publication date: Available online 14 July 2017
      Source:Acta Histochemica
      Author(s): Xi Zeng, Mao Li, Guang-dong Liao, Ming-Rong Xi

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.005
  • The effects of estradiol valerate and remifemin on liver lipid metabolism
    • Authors: Biao Jin; Wenjuan Wang; Wenpei Bai; Jing Zhang; Ke Wang; Lihua Qin
      Abstract: Publication date: Available online 11 July 2017
      Source:Acta Histochemica
      Author(s): Biao Jin, Wenjuan Wang, Wenpei Bai, Jing Zhang, Ke Wang, Lihua Qin
      To investigate the lipid metabolism dysregulation in the liver of ovariectomized (OVX) rats and effects of estradiol valerate (E) and remifemin (ICR) thereon, forty female Sprague-Dawley rats were randomly divided into sham-operated (SHAM), OVX, OVX+E, and OVX+ICR group. After 4 weeks’ E or ICR treatment, serum estrogen, cholesterol, and triglyceride levels; lipid droplets in hepatocytes; hepatocyte morphology; and the expression of estrogen receptor α (ERα), liver X receptor (LXR), and sterol regulatory element binding proteins (SREBPs) in the liver of the rats were assessed. OVX rats had significantly decreased serum estrogen levels, which significantly increased after treatment with E but not with ICR. Serum triglyceride levels and the amount of lipid droplets in hepatocytes increased after ovariectomy, and significantly decreased after E treatment. In addition, ICR treatment markedly increased serum triglyceride levels and lipid droplet size. No significant differences in the serum cholesterol levels were observed among the four groups. After ovariectomy, hepatocyte mitochondria became hypertrophic and misformed, which were reversed with E or ICR treatment. ICR-treated rats also showed endoplasmic reticulum disorganization. After ovariectomy, ERα and LXR levels significantly decreased while SREBP expression increased. E treatment increased ERα and LXR levels while ICR treatment only increased LXR expression. E treatment decreased SREBP-1c levels, whereas SREBP-1c levels increased with ICR treatment. Treatment with E significantly reversed the ovariectomy-induced dysregulation of hepatocyte lipid metabolism, which was, however, exacerbated with ICR treatment. The effects of E and ICR on hepatocyte lipid metabolism may involve the regulation of LXR and SREBP-1c.

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.004
  • Cells in 3D-reconstitutued eccrine sweat gland cell spheroids
           differentiate into gross cystic disease fluid protein 15-expressing dark
           secretory cells and carbonic anhydrase II-expressing clear secretory cells
    • Authors: Haihong Li; Liyun Chen; Mingjun Zhang; Bingna Zhang
      Abstract: Publication date: Available online 6 July 2017
      Source:Acta Histochemica
      Author(s): Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
      Secretory coils of eccrine sweat glands are composed of myoepithelial cells, dark secretory cells and clear secretory cells. The two types of cells play important roles in sweat secretion. In our previous study, we demonstrated that the 3D-reconstituted eccrine sweat gland cell spheroids differentiate into secretory coil-like structures. However, whether the secretory coil-like structures further differentiate into dark secretory cells and clear secretory cells were is still unknown. In this study, we detected the differentiation of clear and dark secretory cells in the 3D-reconstituted eccrine sweat gland cell spheroids using the dark secretory cell-specific marker, GCDFP-15, and clear secretory cell-specific marker, CAII by immunofluorescence staining. Results showed that there were both GCDFP-15- and CAII-expressing cells in 12-week-old 3D spheroids, similar to native eccrine sweat glands, indicating that the spheroids possess a cellular structure capable of sweat secretion. We conclude that the 12-week 3D spheroids may have secretory capability.

      PubDate: 2017-07-09T16:55:03Z
      DOI: 10.1016/j.acthis.2017.07.001
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Heriot-Watt University
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