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Publisher: Elsevier   (Total: 3048 journals)

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Showing 1 - 200 of 3048 Journals sorted alphabetically
A Practical Logic of Cognitive Systems     Full-text available via subscription   (Followers: 7)
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 25, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 22, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 86, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 25, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 30, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 361, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 1)
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 226, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 10, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 1)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 24, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 4, SJR: 0.383, h-index: 19)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 2)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 4)
Acute Pain     Full-text available via subscription   (Followers: 13)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 6)
Additive Manufacturing     Hybrid Journal   (Followers: 7, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 21)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 135, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 17, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 26, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 11, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 22, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 25, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 2.139, h-index: 42)
Advances in Cell Aging and Gerontology     Full-text available via subscription   (Followers: 4)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 27, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 9, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 29, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Dermatology     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 6)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 44, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 26, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 42, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 50, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 16)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 12)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 22, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 26)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 36, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 6)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 9)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 8, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organ Biology     Full-text available via subscription   (Followers: 2)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 24, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 15, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 17)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 62)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 2, SJR: 0.1, h-index: 2)
Advances in Space Biology and Medicine     Full-text available via subscription   (Followers: 5)
Advances in Space Research     Full-text available via subscription   (Followers: 361, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 7, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 30, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 16)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 44, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 327, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 5, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 8, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 413, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 16, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 30, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 40, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 55, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 11, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 8)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access   (Followers: 1)
Algal Research     Partially Free   (Followers: 8, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
Alpha Omegan     Full-text available via subscription   (SJR: 0.121, h-index: 9)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 9, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 46, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 4)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 4)
Ambulatory Pediatrics     Hybrid Journal   (Followers: 5)
American Heart J.     Hybrid Journal   (Followers: 48, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 47, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 40, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 9, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 14, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 32, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 26, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 32, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 46, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 201, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 59, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 6)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 25, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 26, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 24, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 35, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 58, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 12)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 4, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 37, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 167, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 12)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 1)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 23, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 172, SJR: 1.907, h-index: 126)

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Journal Cover Advances in Medical Sciences
  [SJR: 0.489]   [H-I: 25]   [6 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1896-1126 - ISSN (Online) 1898-4002
   Published by Elsevier Homepage  [3048 journals]
  • The alterations in alcohol dehydrogenase and aldehyde dehydrogenase
           activities in the sera of patients with renal cell carcinoma
    • Authors: Karolina Orywal; Wojciech Jelski; Tadeusz Werel; Maciej Szmitkowski
      Pages: 1 - 4
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Karolina Orywal, Wojciech Jelski, Tadeusz Werel, Maciej Szmitkowski
      Purpose In a previous study we showed that the total activity of alcohol dehydrogenase (ADH) and its isoenzyme class I was significantly higher in renal cancer (RCC) cells compared to normal kidney. The aim of this study was to compare the activities of ADH isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with different stages of RCC and healthy subjects. Materials and methods Serum samples were taken from 54 patients with clear cell RCC (17 patients in stage II, 22 in stage III and 15 in stage IV) and 52 healthy patients. Class III, IV of ADH and the total ADH activity was measured by the photometric method. For the measurement of ADH class I, II and the total ALDH activity we employed the fluorometric method. Results The total activity of ADH and its isoenzyme class I were significantly higher in the sera of patients with every stage of RCC compared to healthy subjects. The analysis of ALDH activity did not indicate significant differences between tested groups. Conclusions The increased activity of total ADH and its isoenzyme class I in the sera of patients with RCC, seems to be caused by isoenzymes being released from cancerous cells and may be useful for diagnostics of renal cancer.

      PubDate: 2017-08-06T13:01:40Z
      DOI: 10.1016/j.advms.2017.05.001
       
  • Impact of intervention on metabolic outcomes among dropouts with type 2
           diabetes
    • Authors: Merja K. Laine; Timo Kauppila; Mikko Honkasalo; Marko Raina; Johan G. Eriksson
      Pages: 5 - 8
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Merja K. Laine, Timo Kauppila, Mikko Honkasalo, Marko Raina, Johan G. Eriksson
      Purpose The aim of this study was to evaluate the effect of an individual intervention given by health care professionals to dropouts with type 2 diabetes (T2D) on their metabolic profile. Materials/methods In 2010, we identified 356 T2D dropouts in Vantaa Health Centre, Finland. At the baseline visit the participants’ status was assessed including laboratory tests. Diabetes counseling was given, and drug treatment was enhanced when needed. The follow-up visit was performed 13 to 30 months later including the same assessments as performed at the baseline visit. The dropouts who attended the follow-up visit formed the study group. One third (n=115) of the dropouts participated in the follow-up visit. Results The study participants (mean age 61.4 years) were older than the non-participants (mean age 58.5 years) (p=0.009). After the intervention the proportion of participants with hemoglobin A1c≥9% (75mmol/mol) decreased from 15.5% to 5.2% (p=0.004). Improvements were also observed in general in hemoglobin A1c, from 6.6% (49mmol/mol) to 6.3% (45mmol/mol) (p=0.001), in total cholesterol, from 4.9mmol/l to 4.5mmol/l (p=0.011), in low-density lipoprotein cholesterol, from 2.9mmol/l to 2.6mmol/l (p=0.015) and in diastolic blood pressure, from 90mmHg to 84mmHg (p=0.001). Conclusions Dropouts with T2D were difficult to bring back to the public health care system, especially men under the age of 60 years. Dropouts who participated in the intervention showed improvements in several metabolic outcomes.

      PubDate: 2017-08-06T13:01:40Z
      DOI: 10.1016/j.advms.2017.05.003
       
  • Genetic basis of enzymatic resistance of E. coli to aminoglycosides
    • Authors: Dominika Ojdana; Anna Sieńko; Paweł Sacha; Piotr Majewski; Piotr Wieczorek; Anna Wieczorek; Elżbieta Tryniszewska
      Pages: 9 - 13
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Dominika Ojdana, Anna Sieńko, Paweł Sacha, Piotr Majewski, Piotr Wieczorek, Anna Wieczorek, Elżbieta Tryniszewska
      Purpose Over the past years, an increase in resistance to aminoglycosides has been observed among Enterobacteriaceae rods. This resistance development reduces therapeutic options for infections caused by multidrug-resistance organisms. Because of the changing epidemiology of extended-spectrum β-lactamases (ESBLs) and resistance to aminoglycosides, we investigated the prevalence of the aac(3)-Ia, aac(6′)-Ib, ant(4′)-IIa, ant(2”)-Ia, and aph(3”)-Ib genes encoding aminoglycoside-modifying enzymes (AMEs) in ESBL-producing Escherichia coli as well as ESBL-non-producing isolates. To understand bacterial resistance to aminoglycoside antibiotics, we estimated resistance phenotypes and the presence of genes responsible for this resistance. Materials and methods The study was conducted on 44 E.coli strains originated from patients hospitalized at University Hospital of Bialystok. MIC values were obtained for gentamicin, amikacin, netilmicin, and tobramycin. Isolates were tested for the presence of the aac(3)-Ia, aac(6′)-Ib, ant(4′)-IIa, ant(2”)-Ia, and aph(3”)-Ib genes with the use of the PCR technique. Results Resistance to aminoglycosides was found in 79.5% of the isolates. The highest percentages of resistance were observed for tobramycin (70,5%) and gentamicin (59%), followed by netilmicin (43.2%) and amikacin (11.4%). PCR assays revealed the presence of aac(6′)-Ib among 26 (59.2%) strains, aph(3”)-Ib among 16 (36.2%), aac(3)-Ia among 7 (15.9%), and ant(2”)-Ia among 2 (4.6%) strains. Conclusions The enzymatic resistance against aminoglycosides in northeastern Poland among clinical isolates of E. coli is predominantly caused by aac(6′)-Ib and aph(3”)-Ib. Amikacin may be used for therapy of infections caused by ESBL-producing E. coli, because of the low rates of resistance.

      PubDate: 2017-08-06T13:01:40Z
      DOI: 10.1016/j.advms.2017.05.004
       
  • A systematic review on the role of eicosanoid pathways in rheumatoid
           arthritis
    • Authors: Malvina Hoxha
      Pages: 22 - 29
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Malvina Hoxha
      Background Rheumatoid arthritis is characterized by the production of eicosanoids, cytokines, adhesion molecules, infiltration of T and B lymphocytes in the synovium and oxygen reduction accompanied by the cartilage degradation. Eicosanoids are responsible for the progressive destruction of cartilage and bone, however neither steroids, nor the non steroidal anti-inflammatory drugs (NSAIDs), cannot slow down cartilage and bone destruction providing only symptomatic improvement. The current rheumatoid arthritis treatment options include mainly the use of disease-modifying anti-rheumatic drugs, the corticosteroids, the NSAIDs and biological agents. Methods PubMed, Cochrane, and Embase electronic database were used as the main sources for extracting several articles, reviews, original papers in English for further review and analysis on the implication of arachidonic acid metabolites with rheumatoid arthritis and different strategies of targeting arachidonic acid metabolites, different enzymes or receptors for improving the treatment of rheumatoid arthritis patients. Results We first focused on the role of individual prostaglandins and leukotrienes, in the inflammatory process of arthritis, concluding with an outline of the current clinical situation of rheumatoid arthritis and novel treatment strategies targeting the arachidonic acid pathway. Conclusions Extended research is necessary for the development of these novel compounds targeting the eicosanoid pathway, by increasing the levels of anti-inflammatory eicosanoids (PGD2,15dPGJ2), by inhibiting the production of pro-inflammatory eicosanoids (PGE2, LTB4, PGI2) involved in rheumatoid arthritis or also by developing dual compounds displaying both the COX-2 inhibitor/TP antagonist activity within a single compound.

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.06.004
       
  • Patients with atrial fibrillation and coronary artery disease –
           Double trouble
    • Authors: Ewelina Michniewicz; Elżbieta Mlodawska; Paulina Lopatowska; Anna Tomaszuk-Kazberuk; Jolanta Malyszko
      Pages: 30 - 35
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Ewelina Michniewicz, Elżbieta Mlodawska, Paulina Lopatowska, Anna Tomaszuk-Kazberuk, Jolanta Malyszko
      Coronary artery disease (CAD) is the most common cardiovascular disease while atrial fibrillation (AF) is the most common cardiac arrhythmia. Both diseases share associated risk factors – hypertension, diabetes mellitus, sleep apnea, obesity and smoking. Moreover, inflammation plays a causative role in both diseases. The prevalence of CAD in patients with AF is from 17% to 46.5% while the prevalence of AF among patients with CAD is low and it is estimated from 0.2% to 5%. AF is a well-established factor of poor short- and long-term prognosis in patients with acute myocardial infarction (AMI) and is associated with a marked increase in overall mortality. The arrhythmia is common after cardiac surgeries and occurs in about 20 to 40% of patients after coronary artery bypass graft (CABG) surgery. It is predicted that between 5 and 15% of AF patients will require stenting at some point in their lives and will receive triple therapy with aspirin, clopidogrel or ticagrelor and oral anticoagulation (OAC). This requires careful consideration of antithrombotic therapy, balancing bleeding risk, stroke risk, and in-stent thrombosis with subsequent acute coronary syndromes. Co-prescription of OAC with antiplatelet therapy, in particular triple therapy, increases the absolute risk of major bleeding. In addition, major bleeding is associated with an up to 5-fold increased risk of death following an acute coronary syndrome. Coexistence of AF and CAD worsens the prognosis even in carefully treated patients.

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.06.005
       
  • Antidepressant-like activity of methyl jasmonate involves modulation of
           monoaminergic pathways in mice
    • Authors: Solomon Umukoro; Adaeze Adebesin; Gladys Agu; Osarume Omorogbe; Stephen Babajide Asehinde
      Pages: 36 - 42
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Solomon Umukoro, Adaeze Adebesin, Gladys Agu, Osarume Omorogbe, Stephen Babajide Asehinde
      Purpose The efficacy of current antidepressant drugs has been compromised by adverse effects, low remission and delay onset of action necessitating the search for alternative agents. Methyl jasmonate (MJ), a bioactive compound isolated from Jasminum grandiflorum has been shown to demonstrate antidepressant activity but its mechanism of action remains unknown. Thus, the role of monoaminergic systems in the antidepression-like activity of MJ was investigated in this study. Materials and methods Mice were given i.p. injection of MJ (5, 10 and 20mg/kg), imipramine (10mg/kg) and vehicle (10mL/kg) 30min before the forced swim test (FST) and tail suspension test (TST) were carried out. The involvement of monoaminergic systems in the anti-depressant-like effect of MJ (20mg/kg) was evaluated using p-chlorophenylalanine (pCPA), metergoline, yohimbine, prazosin, sulpiride and haloperidol in the TST. Results MJ significantly decrease the duration of immobility in the FST and TST relative to control suggesting antidepressant-like property. However, pretreatment with yohimbine (1mg/kg, i.p., an α2-adrenergic receptor antagonist) or prazosin (62.5μg/kg, i.p., an α1-adrenoceptor antagonist) attenuated the antidepressant-like activity of MJ. Also, pCPA; an inhibitor of serotonin biosynthesis (100mg/kg, i.p) or metergoline (4mg/kg, i.p., 5-HT2 receptor antagonist) reversed the anti-immobility effect of MJ. Sulpiride (50mg/kg, i.p., a D2 receptor antagonist) or haloperidol (0.2mg/kg, i.p., a dopamine receptor antagonist) reversed the anti-immobility effect of MJ. Conclusion The results of this study suggest that serotonergic, noradrenergic and dopaminergic systems may play a role in the antidepressant-like activity of MJ.

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.07.005
       
  • Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces
           stress in J774A.1 murine macrophages
    • Authors: Abbas Mohammadi; Zakaria Vahabzadeh; Soran Jamalzadeh; Tahereh Khalili
      Pages: 57 - 63
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Abbas Mohammadi, Zakaria Vahabzadeh, Soran Jamalzadeh, Tahereh Khalili
      Purpose Trimethylamine N-oxide (TMAO) is a biomarker for kidney problems. It has also been introduced as a risk factor for atherosclerosis. The classic risk factors for atherosclerosis trigger cellular and humeral immunoreaction in macrophages through induction of heat shock protein expressions and increased levels of GRP94 and HSP70 are associated with increased atherosclerosis risk. The present study evaluated the possible effect(s) of TMAO on the expression of GRP94 and HSP70 at protein levels. Methods J774A.1 murine macrophages were treated with different micromolar concentrations of TMAO and 4-phenylbutyric acid (PBA), a chemical chaperone, for 8, 18, 24, and 48h intervals. Tunicamycin was also used as a control for induction of endoplasmic reticulum stress. Western blotting was used to evaluate the expression of GRP94 and HSP70 in macrophages at protein levels. Result Tunicamycin greatly increased protein levels of GRP94. Similarly, but to a lesser extent compared to tunicamycin, TMAO also increased GRP94. In 24h treated cells, only 300μM of TMAO, and in cells treated for 48h, all doses of TMAO produced a significant increase in relative HSP70 protein levels compared to the control. PBA failed to induce any changes in GRP94 or HSP70 protein levels. Conclusion GRP94 and HSP70 are stress-inducible heat shock protein, so the elevation in J774A.1 murine macrophages can clearly define cells under stress and elucidate the contribution of stress induced by TMAO that may have a part in the abnormal activation of macrophages involved in foam cell formation.

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.06.006
       
  • The influence of low level laser irradiation on vascular reactivity
    • Authors: Magdelena Mackiewicz-Milewska; Elżbieta Grześk; Andrzej C. Kroszczyński; Małgorzata Cisowska-Adamiak; Hanna Mackiewicz-Nartowicz; Lilianna Baran; Iwona Szymkuć-Bukowska; Michał Wiciński; Wojciech Hagner; Grzegorz Grześk
      Pages: 64 - 67
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Magdelena Mackiewicz-Milewska, Elżbieta Grześk, Andrzej C. Kroszczyński, Małgorzata Cisowska-Adamiak, Hanna Mackiewicz-Nartowicz, Lilianna Baran, Iwona Szymkuć-Bukowska, Michał Wiciński, Wojciech Hagner, Grzegorz Grześk
      Introduction The mechanism of action of low level laser irradiationon on tissues is unclear.
      Authors of publications present the positive clinical impact of low and medium power laser irradiation on vascular reactivity

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.06.002
       
  • Endogenous non-enzymatic antioxidants in the human body
    • Authors: Iwona Mirończuk-Chodakowska; Anna Maria Witkowska; Małgorzata Elżbieta Zujko
      Pages: 68 - 78
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Iwona Mirończuk-Chodakowska, Anna Maria Witkowska, Małgorzata Elżbieta Zujko
      The exposure of cells, tissues and extracellular matrix to harmful reactive species causes a cascade of reactions and induces activation of multiple internal defence mechanisms (enzymatic or non-enzymatic) that provide removal of reactive species and their derivatives. The non-enzymatic antioxidants are represented by molecules characterized by the ability to rapidly inactivate radicals and oxidants. This paper focuses on the major intrinsic non-enzymatic antioxidants, including metal binding proteins (MBPs), glutathione (GSH), uric acid (UA), melatonin (MEL), bilirubin (BIL) and polyamines (PAs).

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.05.005
       
  • A similar pro/anti-inflammatory cytokine balance is present in the airways
           of competitive athletes and non-exercising asthmatics
    • Authors: Marcin Kurowski; Janusz Jurczyk; Agnieszka Olszewska-Ziąber; Marzanna Jarzębska; Hubert Krysztofiak; Marek L. Kowalski
      Pages: 79 - 86
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Marcin Kurowski, Janusz Jurczyk, Agnieszka Olszewska-Ziąber, Marzanna Jarzębska, Hubert Krysztofiak, Marek L. Kowalski
      Purpose Intensive exercise modifies airway inflammation and infection susceptibility. We aimed to determine the effect of exercise on pro- and anti-inflammatory cytokine (TNF-α, IL-1ra, IL-10) and innate immunity protein (HSPA1, sCD14) levels in exhaled breath condensate (EBC) and nasal secretions of competitive athletes, non-exercising asthmatics and healthy controls (HC). Material and methods The study group consisted of 15 competitive athletes (five speed skaters and ten swimmers) aged 15–25. The control groups comprised 10 mild-to-moderate asthmatics (AC) and seven HC. Athletes were assessed in- and off-training while asthmatics and controls at one time point. Nasal lavages and EBC were collected before and after a treadmill exercise challenge. Protein levels were assessed using ELISA. Results TNF-α levels in EBC were significantly higher in athletes than HC, but similar to asthmatic patients. In contrast, IL-1ra EBC concentrations were significantly lower in athletes than in HC, but again similar to asthmatics. Significant positive correlations were seen between baseline concentrations of TNF-α in EBC and fall in FEV1 following exercise challenge in athletes during training period (R=0.74, p<0.01) and in asthmatics (R=0.64, p<0.05). In nasal secretions, baseline IL-1ra levels were significantly higher in athletes and asthmatics than in HC. Exercise caused a slight, yet significant, increase in EBC HSPA1 in athletes (p=0.02). The exercise challenge did not considerably influence TNF-α, IL-1ra, HSPA1 and sCD14 in EBC or nasal secretions. Conclusions Dysregulation of the TNF-α/IL-1ra balance in EBC and nasal secretions from athletes may reflect the presence of airway inflammation induced by repeated strenuous exercise.

      PubDate: 2017-08-27T21:15:05Z
      DOI: 10.1016/j.advms.2017.07.004
       
  • Novel mutations and their genotype-phenotype correlations in patients with
           Noonan syndrome, using next-generation sequencing
    • Authors: Alireza Tafazoli; Peyman Eshraghi; Francesca Pantaleoni; Rahim Vakili; Morteza Moghaddassian; Martha Ghahraman; Valentina Muto; Stefano Paolacci; Fatemeh Fardi Golyan; Mohammad Reza Abbaszadegan
      Pages: 87 - 93
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Alireza Tafazoli, Peyman Eshraghi, Francesca Pantaleoni, Rahim Vakili, Morteza Moghaddassian, Martha Ghahraman, Valentina Muto, Stefano Paolacci, Fatemeh Fardi Golyan, Mohammad Reza Abbaszadegan
      Purpose Noonan Syndrome (NS) is an autosomal dominant disorder with many variable and heterogeneous conditions. The genetic basis for 20–30% of cases is still unknown. This study evaluates Iranian Noonan patients both clinically and genetically for the first time. Materials/methods Mutational analysis of PTPN11 gene was performed in 15 Iranian patients, using PCR and Sanger sequencing at phase one. Then, as phase two, Next Generation Sequencing (NGS) in the form of targeted resequencing was utilized for analysis of exons from other related genes. Homology modelling for the novel founded mutations was performed as well. The genotype, phenotype correlation was done according to the molecular findings and clinical features. Results Previously reported mutation (p.N308D) in some patients and a novel mutation (p.D155N) in one of the patients were identified in phase one. After applying NGS methods, known and new variants were found in four patients in other genes, including: CBL (p. V904I), KRAS (p. L53W), SOS1 (p. I1302V), and SOS1 (p. R552G). Structural studies of two deduced novel mutations in related genes revealed deficiencies in the mutated proteins. Following genotype, phenotype correlation, a new pattern of the presence of intellectual disability in two patients was registered. Conclusions NS shows strong variable expressivity along the high genetic heterogeneity especially in distinct populations and ethnic groups. Also possibly unknown other causative genes may be exist. Obviously, more comprehensive and new technologies like NGS methods are the best choice for detection of molecular defects in patients for genotype, phenotype correlation and disease management.

      PubDate: 2017-09-30T16:22:38Z
      DOI: 10.1016/j.advms.2017.07.001
       
  • Pediatric reference data on activity of urinary
           N-acetyl-β-D-hexosaminidase and its isoenzymes
    • Authors: B. Zalewska-Szajda; K. Taranta-Janusz; S. Chojnowska; N. Waszkiewicz; K. Zwierz; A. Wasilewska
      Pages: 94 - 99
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): B. Zalewska-Szajda, K. Taranta-Janusz, S. Chojnowska, N. Waszkiewicz, K. Zwierz, A. Wasilewska
      Purpose The objective of the study was to establish age − dependent values of the urinary lysosomal exoglycosidases activities: N-acetyl-β-D-hexosaminidase (HEX) and its isoenzyme A (HEX A) as well as isoenzyme B (HEX B) in healthy children and adolescents. Material and methods The study was performed using a random sample of 203 healthy children and adolescents (girls=99, boys=104), aged six months to 17.9 years. The activities of HEX, HEX A and HEX B were determined by a colorimetric method. The activities of the urinary HEX and its isoenzymes were expressed in pKat/μg of creatinine (pKat/μg Cr). Results Median concentrations of urinary HEX, and its HEX A, HEX B isoenzymes in particular age groups were analyzed using ANOVA. Urinary HEX, HEX A and HEX B activities (pKat/μg Cr) were the highest in children below 3 years, in comparison to remaining age groups. There were statistically significant negative correlations between urinary HEX, HEX A as well as HEX B and age (r=−0.24, p<0.001 (HEX); r=−0.20, p<0.01 (HEX A); r=−0.26, p<0.001 (HEX B), respectively. We constructed the reference values for urinary activity of HEX, HEX A and HEX B (pKat/μg Cr) in centiles according to age, in three-year intervals. Conclusions Reported data present, for the first time, reference values for urinary activities of HEX and its isoenzymes HEX A and HEX B in children and adolescent.

      PubDate: 2017-09-06T00:04:35Z
      DOI: 10.1016/j.advms.2017.06.007
       
  • Pharmacological thrombolysis for acute ischemic stroke treatment: Gender
           differences in clinical risk factors
    • Authors: Michael J. Colello; Lauren E. Ivey; Jordan Gainey; Rakiya V. Faulkner; Ashleigh Johnson; Leanne Brechtel; Lee Madeline; Thomas I. Nathaniel
      Pages: 100 - 106
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Michael J. Colello, Lauren E. Ivey, Jordan Gainey, Rakiya V. Faulkner, Ashleigh Johnson, Leanne Brechtel, Lee Madeline, Thomas I. Nathaniel
      Background In a stroke population, women have a worse outcome than men when untreated. In contrast, there is no significant difference in treated patients. In this study, we determined whether clinical variables represent a promising approach to assist in the evaluation of gender differences in a stroke population. Methods We analyzed data from ischemic stroke patients’ ≥18 years-old from the stroke registry on rtPA administration and identified gender differences in clinical factors within inclusion and exclusion criteria in a stroke population that received rtPA. Multivariate analysis was used to adjust for patient demographic and clinical variables. Results Of the 241 eligible stroke patients’ thrombolytic therapy, 49.4% were females and 50.6% were males. Of the 422 patients that did not receive rtPA, more women (235) were excluded from rtPA than men (187) (P<0.05). In the male population, exclusion from rtPA was associated with history of a previous stroke (P<0.05, OR=2.028), hypertension (P<0.05, OR=0.519), and NIH stroke score (P<0.0001, OR=0.893). In female stroke patients, exclusion from rtPA was associated with previous history of stroke (P<0.05, OR=2.332), diabetes (P<0.05, OR=1.88) and NIH stroke score (P<0.05, OR=0.916). Conclusions Despite similarities in different areas of stroke care for both men and women, more women with diabetes, previous history of stroke and higher NIH scores are more likely to be excluded from thrombolytic therapy. Men with a previous history of stroke, hypertension and higher NIH scores are more likely to be excluded rtPA even after adjustment for confounding variables.

      PubDate: 2017-10-09T18:49:41Z
      DOI: 10.1016/j.advms.2017.08.003
       
  • Physeal fractures of the lower leg in children and adolescents:
           Therapeutic results, pitfalls and suggested management protocol-based on
           the experience of the authors and contemporary literature
    • Authors: Marcin Karlikowski; Jerzy Sułko
      Pages: 107 - 111
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Marcin Karlikowski, Jerzy Sułko
      Introduction Physeal fractures in children frequently give rise to concerns about the condition of the growth plate. Our observations have proven that the dysfunction of the growth plate is less frequent complication in those cases than misdiagnosed interposition of the periosteum. The aim of this paper is to familiarize the readers with the issue of treatment of physeal fractures of the distal tibia and fibula in the growing skeleton. Materials and methods We analyzed the group of 75 patients – children and adolescents – with surgically treated physeal fractures of the lower leg. The analysis included age, sex, circumstances of trauma infliction, type of sustained damage, employed therapeutic technique, timing of surgical procedure, duration of hospitalization, complications, duration of follow-up, radiological and functional results according to the AOFAS scale. Results The group consisted of 23 girls and 52 boys. The mean age was 13.6 years. The most frequent cause of trauma was same-level fall, usually during sports activities (35 cases). The most common type of damage was Salter-Harris type II fracture (35 cases). Among the employed surgical techniques, open reduction and stabilization with K-wires was the most often used (52 cases). A group of four patients attracted our attention, in whom after a closed reduction, signs of periosteum interposition were noted. These patients required a second procedure. In one patient, the growth plate arrest occurred; it was directly caused by local osteomyelitis. Conclusions With adequately conducted treatment of distal tibia and distal fibula physeal fractures, the results are good. Misdiagnosed periosteum interposition poses a more serious clinical problem as opposed to the commonly anxiety-provoking post-traumatic growth plate dysfunction.

      PubDate: 2017-10-29T01:59:58Z
      DOI: 10.1016/j.advms.2017.10.001
       
  • Monitoring both procalcitonin and C-reactive protein in the early period
           after tetralogy of Fallot correction in children promotes rational
           antibiotic use
    • Authors: Radoslaw Jaworski; Ireneusz Haponiuk; Ninela Irga-Jaworska; Mariusz Steffens; Maciej Chojnicki; Konrad Paczkowski; Jacek Zielinski
      Pages: 112 - 118
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Radoslaw Jaworski, Ireneusz Haponiuk, Ninela Irga-Jaworska, Mariusz Steffens, Maciej Chojnicki, Konrad Paczkowski, Jacek Zielinski
      Purpose This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. The ability of these inflammatory markers to guide rational antibiotic usage was also determined. Materials and Methods All consecutive children who underwent ToF correction in 2009–2016 in our referral pediatric cardiac surgery clinic in Gdansk, Poland and did not exhibit infection signs on early postoperative days (POD) were identified. All patients received 48h antibiotic prophylaxis. Antibiotic treatment was extended or empirical antibiotic therapy was introduced if the clinician considered it necessary. CRP and PCT levels were measured on POD1–4 and 1–3, respectively. Results Of the 60 eligible children, 44 underwent CRP testing only. The remaining 16 patients underwent both CRP and PCT testing. All patients had abnormally high CRP values after surgery. All patients who also underwent PCT testing also displayed elevated PCT levels. The CRP and PCT levels peaked on POD2 (median=99.8mg/L) and POD1 (median=4.08ng/mL), respectively. In the CRP-alone patients, antibiotic prophylaxis was prolonged or empirical antibiotic therapy was started in 59%; in the CRP and PCT group, this was 25% (p<0.05). Conclusions The children had elevated CRP and PCT levels after ToF correction, with peaks observed on POD2 and POD1, respectively. Monitoring both CRP and PCT in the early postoperative period may guide antibiotic therapy, thus reducing unnecessary treatment, additional toxicity, and adverse drug interactions without increasing treatment failure. Rational antibiotic treatment may also reduce antibiotic resistance.

      PubDate: 2017-11-20T04:09:31Z
      DOI: 10.1016/j.advms.2017.10.003
       
  • Effect of gestational age on migration ability of the human umbilical cord
           vein mesenchymal stem cells
    • Authors: Mobin Mohammadi; Mehdi Mohammadi; Mohammad Ali Rezaee; Tayyeb Ghadimi; Massume Abolhasani; Mohammad Reza Rahmani
      Pages: 119 - 126
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Mobin Mohammadi, Mehdi Mohammadi, Mohammad Ali Rezaee, Tayyeb Ghadimi, Massume Abolhasani, Mohammad Reza Rahmani
      Purpose Migration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant factor in cell therapy. MSCs derived from different sources show different properties. Here we compared the migration ability of the term and the pre-term human umbilical cord vein MSCs (hUCV-MSCs). Materials/Methods MSCs were isolated from term and pre-term umbilical cord vein, and cultured to passage 3–4. Migration rate of both groups was assessed in the presence of 10% FBS using chemotaxis assay. Surface expression of CXCR4 was measured by flow cytometery. The relative gene expression of CXCR4, IGF1-R, PDGFRα, MMP-2, MMP-9, MT1-MMP and TIMP-2 were evaluated using real time PCR. Results The isolation rate of the pre-term hUCV-MSCs was higher than the term hUCV-MSCs. Phenotype characteristics and differentiation ability of the term and pre-term hUCV-MSCs were not different. The migration rate of the pre-term hUCV-MSCs was more than the term hUCV-MSCs. Gene and surface expressions of the CXCR4 were both significantly higher in the pre-term hUCV-MSCs (P≤0.05). The mRNA levels of PDGFRα, MMP-2, MMP-9, MT1-MMP and TIMP-2 showed no significant difference between the two groups. Conclusion Our results showed that the gestational age can affect the migration ability of the hUCV-MSCs.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.08.002
       
  • Ultrastructural characteristics of the respective forms of hepatic
           stellate cells in chronic hepatitis B as an example of high fibroblastic
           cell plasticity. The first assessment in children
    • Authors: Joanna Maria Lotowska; Maria Elzbieta Sobaniec-Lotowska; Dariusz Marek Lebensztejn
      Pages: 127 - 133
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Joanna Maria Lotowska, Maria Elzbieta Sobaniec-Lotowska, Dariusz Marek Lebensztejn
      Purpose Activation of hepatic stellate cells (HSCs), mainly responsible for extracellular matrix synthesis, is assumed to be central event in the process of liver fibrogenesis. The major objective of the research was to analyze the ultrastructural profile of activated HSCs in children with chronic hepatitis B (chB), with respect to fibrosis intensity. Materials/methods Ultrastructural investigations of HSCs were conducted on liver bioptates from 70 children with clinicopathologically diagnosed chB before antiviral treatment. Biopsy material, fixed in paraformaldehyde and glutaraldehyde solution, was routinely processed for electron-microscopic analysis. Results In children with intensive liver fibrosis (S-2 and S-3), the ultrastructural picture showed almost total replacement of quiescent HSCs (Q-HSCs) by activated, i.e. transitional HSCs (T-HSCs). Among T-HSCs, two types of cells were distinguished: cells exhibiting initiation of HSC activation (Ti-HSCs), never before described in chB, that were frequently accompanied by activated Kupffer cells, and cells with features of perpetuation of activation (Tp-HSCs). Tp-HSCs were elongated and characterized by substantial loss of cytoplasmic lipid material; they contained an increased number of cytoskeletal components, extremely dilated channels of granular endoplasmic reticulum and activated Golgi apparatus, which indicated their marked involvement in intensive synthesis of extracellular matrix proteins. Many collagen fibers were found to adhere directly to Tp-HSCs. Conclusions The current study showed T-HSCs to be an important link between Q-HSCs and myofibroblastic HSCs (Mf-HSCs). Transformation of HSCs into new morphological variations (Ti-HSCs; Tp-HSCs and Mf-HSCs), observed along with growing fibrosis, indicates their high plasticity and a key role in fibrogenesis in pediatric chB.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.09.002
       
  • The protective effects of Bacillus licheniformis preparation on
           gastrointestinal disorders and inflammation induced by radiotherapy in
           pediatric with central nervous system tumor
    • Authors: Shu-Xu Du; Yong-Rui Jia; Si-Qi Ren; Xiao-Jun Gong; Hong Tang; Wu Wan-Shui; Sun Li-Ming
      Pages: 134 - 139
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Shu-Xu Du, Yong-Rui Jia, Si-Qi Ren, Xiao-Jun Gong, Hong Tang, Wu Wan-Shui, Sun Li-Ming
      Purpose we studied the effect of Bacillus licheniformis preparation (ZCS) on CNST (central nervous system tumor) patients undergoing the gastrointestinal symptoms and inflammation induced by radiotherapy. Materials and Methods 160 CNST patients with craniospinal irradiation (CSI) treatment were divided into experiment and control group. The experiment group patients took one capsule per time of ZCS and three times a day until the end of radiotherapy, starting one day before radiotherapy. While the patients in control group were administrated placebo without any probiotics. Serum from one day before radiotherapy and the first day after radiotherapy were collected to measure the ET, CRP, TNF-α, IL-1β and IL-6. Results More than 70% CNST pediatric patients suffered from different degrees of gastrointestinal symptoms after radiotherapy, including mouth ulcer, nausea, vomiting, abdominal pain and diarrhea. And there was an obviously increased of serum ET, TNF-α, IL-1β, IL-6 and CRP after RT. Importantly, a markedly decreased of ET, CRP and inflammatory cytokines were detected in the experiment group comparing to the control group after radiotherapy, as well as the relief of the gastrointestinal symptoms. However, improvement of probiotics (or ZCS) of the survival rate of CNST children and the recurrence of tumor are not observed in this study. Conclusions Prophylactically administrated ZCS during radiotherapy for CNST patients can relieve RT-related gastrointestinal symptoms and inflammatory reaction.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.09.005
       
  • The application of L-PRP in AIDS patients with crural chronic ulcers: A
           pilot study
    • Authors: Agata Cieslik-Bielecka; Rafał Skowroński; Magdalena Jędrusik-Pawłowska; Marcin Pierchała
      Pages: 140 - 146
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Agata Cieslik-Bielecka, Rafał Skowroński, Magdalena Jędrusik-Pawłowska, Marcin Pierchała
      Nonhealing wounds or skin ulcerations are the result of insufficient repair and destruction of a local healing potential. Opportunistic infections which cause a lot of ulcer complications influence the worsening general condition of patients with AIDS, ultimately leading to death. The chronicity of the condition and poor results of conventional therapy have prompted the search for new methods of treatment. We have examined venous or arteriovenous insufficiency-related extensive crural ulcers in AIDS patients. Crural ulcer healing processes were evaluated with clinical observations and histopathological, immunohistochemical and molecular examinations of tissue samples harvested from the wound edges before and on day 10 after L-PRP cover dressing. Clinical observations showed positive effects of L-PRP in all patients. However, complete wound closure was noted in 60% of cases. Statistical analysis of histological examination showed increased epidermal processes between samples, but the difference was nonsignificant. However, immunohistochemical investigations showed an increased healing process with strong statistical significance. The mean VEGF level before L-PRP usage was 114.3 vessels/mm2 and on day 10 118.9 (p=0.001523). The mean FLK level was 103.2 and 109.9 respectively (p=0.008241). The biggest differences were observed for CD34, with values of 68.2 on day 0 and 100.8 on day 10 (p=0.006982). Molecular analysis generally showed decreased gene expression and confirmed vascular formation and reepithelialization processes. In our opinion, L-PRP may be used to eradicate microorganisms from wounds, to induce neovascularization, and in unhealed cases prepare the base and edge of the ulcer for skin grafting and tissue expansion procedures.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.10.002
       
  • PPARγ Pro12Ala and C161T polymorphisms in patients with acne vulgaris:
           Contribution to lipid and lipoprotein profile
    • Authors: Shohreh Saeidi; Foroogh Chamaie-Nejad; Ali Ebrahimi; Fariba Najafi; Ziba Rahimi; Asad Vaisi-Raygani; Ebrahim Shakiba; Zohreh Rahimi
      Pages: 147 - 151
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Shohreh Saeidi, Foroogh Chamaie-Nejad, Ali Ebrahimi, Fariba Najafi, Ziba Rahimi, Asad Vaisi-Raygani, Ebrahim Shakiba, Zohreh Rahimi
      Purpose The aim of present study was to clarify the role of peroxisome proliferator-activated receptor γ (PPARγ) Pro12Ala and C161T variants in the pathogenesis of acne vulgaris (AV) and their influence on lipid and lipoprotein profile. Methods The present case-control study consisted of 393 individuals including 198 patients with AV (mild-, moderate-, and severe-AV) and 195 unrelated age-matched healthy individuals from Western Iran. The PPARγ Pro12Ala and C161T polymorphisms were identified using polymerase chain reaction-restriction length polymorphism method. Also, serum lipid and lipoprotein profile and fasting blood sugar (FBS) were detected in studied individuals. Results In women patients with AV significantly higher serum levels of FBS, total cholesterol, low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol compared to healthy women were detected. Neither PPARγ Pro12Ala nor C161T polymorphism was associated with the risk of AV but the Pro allele was a risk factor for AV among all men and women patients ≥20years. The variant genotype of PPARγ CG (Pro/Ala) was associated with significantly higher levels of total cholesterol and triglycerides compared to CC (Pro/Pro) genotype. We detected a significantly lower level of FBS in the presence of CT+TT genotype of PPARγ C161T compared to CC genotype. Also, carriers of PPARγ TT genotype had significantly lower serum level of total cholesterol and LDL-C compared to CC genotype. Conclusions Our results demonstrated the association of PPARγ Pro allele with susceptibility to AV in patients ≥20years and the influence of PPARγ Pro12Ala and C161T polymorphisms on the lipid and lipoprotein profile.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.09.003
       
  • The safety and efficacy of light emitting diodes-based ultraviolet A1
           phototherapy in bleomycin-induced scleroderma in mice
    • Authors: Diana Karpec; Romualdas Rudys; Laima Leonaviciene; Zygmunt Mackiewicz; Ruta Bradunaite; Gailute Kirdaite; Algirdas Venalis
      Pages: 152 - 159
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Diana Karpec, Romualdas Rudys, Laima Leonaviciene, Zygmunt Mackiewicz, Ruta Bradunaite, Gailute Kirdaite, Algirdas Venalis
      Purpose To define the efficacy and safety of narrowband ultraviolet A1 (UVA1) for the treatment of dermal fibrosis in bleomycin-induced mouse model of scleroderma. Materials and methods 42 DBA/2 strain mice were included in the study: healthy mice and mice with established scleroderma, treated with high or medium dose of UVA1. Non-treated groups served as control. The equipment emitting 365±5nm UVA1 radiation was used in the study. The average cumulative doses were 1200J/cm2 for high and 600J/cm2 for medium dose course. Histological analysis was performed for the evaluation of the dermal thickness and mast cells density. The expressions of p53 and Ki-67 proteins were assessed by immunohistochemical analyses. Results Skin thickness of mice with scleroderma, treated with high and medium dose of UVA1, were lower (272.9±113.2μm and 394±125.9μm, respectively) in comparison to the dermal thickness of non-treated animals (599±55.7μm). The dermal mast cells count in mice with scleroderma was reduced after high and medium dose treatment to 11±1.7 and 13±2.2, respectively, as compared to that in non-treated mice (23±3.0). No significant upregulation of p53 nor Ki-67 proteins was observed in the skin of healthy mice and mice with scleroderma after high- and medium-dose of UVA1. Conclusions The results of this study indicate that 365nm UVA1 with the cumulative doses of 1200J/cm2 and 600J/cm2 is safe and effective for the dermal fibrosis treatment.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.09.001
       
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A
           link between osteopontin and cardiovascular events
    • Authors: Paweł Rubiś; Sylwia Wiśniowska-Śmiałek; Ewa Dziewięcka; Lucyna Rudnicka-Sosin; Artur Kozanecki; Piotr Podolec
      Pages: 160 - 166
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Paweł Rubiś, Sylwia Wiśniowska-Śmiałek, Ewa Dziewięcka, Lucyna Rudnicka-Sosin, Artur Kozanecki, Piotr Podolec
      Introduction Serum markers of fibrosis provide an insight into extracellular matrix (ECM) fibrosis in heart failure (HF) and dilated cardiomyopathy (DCM). However, their role as predictors of cardiovascular (CV) events in DCM is poorly understood. Methods This is an observational, prospective cohort study. 70 DCM patients (48±12.1years, ejection fraction – EF 24.4±7.4) were recruited. Markers of collagen type I and III synthesis – procollagen type I and III carboxy- and amino-terminal peptides (PICP, PIIICP, PINP, PIIINP), fibrosis controlling factors – ostepontin (OPN), transforming growth factor (TGF1-β) and connective tissue growth factor (CTGF), and matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor (TIMP-1), were measured in serum. All patients underwent endomyocardial biopsy. The end-point was combined with CV death and urgent HF hospitalization. Patients were divided into two groups: those who did (group 1, n=45) and did not reach (group 2, n=25) an end-point. Results Over a 12-month period of observation, 6 CV deaths and 19 HF hospitalizations occurred. Qualitative and quantitative measures of ECM fibrosis were similar in both groups. The levels of all of the markers of collagen synthesis, TGF1-β, MMP-9 and TIMP-1 were similar, however, OPN, CTGF and MMP-2 were significantly lower in group 1. Conclusions Invasively-determined fibrosis levels were not related with CV outcomes in DCM. Out of the 11 markers of fibrosis under study, only OPN was found to be related to CV outcomes. OPN is not only the pivotal protein controlling fibrosis, but may also serve as a biomarker associated with prognosis.

      PubDate: 2017-11-11T11:04:24Z
      DOI: 10.1016/j.advms.2017.10.004
       
  • Tick-borne infections and co-infections in patients with non-specific
           symptoms in Poland
    • Authors: Justyna Dunaj; Anna Moniuszko-Malinowska; Izabela Swiecicka; Martin Andersson; Piotr Czupryna; Krzysztof Rutkowski; Grzegorz Zambrowski; Joanna Zajkowska; Sambor Grygorczuk; Maciej Kondrusik; Renata Świerzbińska; Sławomir Pancewicz
      Pages: 167 - 172
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Justyna Dunaj, Anna Moniuszko-Malinowska, Izabela Swiecicka, Martin Andersson, Piotr Czupryna, Krzysztof Rutkowski, Grzegorz Zambrowski, Joanna Zajkowska, Sambor Grygorczuk, Maciej Kondrusik, Renata Świerzbińska, Sławomir Pancewicz
      Aim The aim of the study was the evaluation of the frequency of infections and co-infections among patients hospitalized because of non-specific symptoms after a tick bite. Materials and methods Whole blood, serum and cerebrospinal fluid samples from 118 patients hospitalised for non-specific symptoms up to 8 weeks after tick bite from 2010 to 2013 were examined for tick-borne infections. ELISA, Western blot and/or molecular biology (PCR; fla gene; 16S rRNA; sequencing) and thin blood smears (MDD) were used. Control group included 50 healthy blood donors. All controls were tested with PCR and serology according to the same procedure as in patients. Results Out of 118 patients 85 (72%) experienced headaches, 15 (13%) vertigo, 32 (27%) nausea, 17 (14%) vomiting, 37 (31%) muscle pain, 73 (62%) fever and 26 (22%) meningeal signs. 47.5% were infected with at least one tick-borne pathogen. Borrelia burgdorferi sensu lato infection was confirmed with ELISA, Western blot in serum and/or (PCR (fla gene) in whole blood in 29.7% cases. In blood of 11.9% patients Anaplasma phagocytophilum DNA (16S rRNA gene) was detected; in 0.9% patients 1/118 Babesia spp. DNA (18S rRNA gene) was also detected. Co-infections were observed in 5.1% of patients with non-specific symptoms. B. burgdorferi s.l. − A. phagocytophilum co-infection (5/118; 4.2%) was most common. In 1/118 (0.8%) A. phagocytophilum – Babesia spp. co-infection was detected. All controls were negative for examined pathogens. Conclusions Non-specific symptoms after tick bite may be caused by uncommon pathogens or co-infection, therefore it should be considered in differential diagnosis after tick bite.

      PubDate: 2017-11-20T04:09:31Z
      DOI: 10.1016/j.advms.2017.09.004
       
  • Vitamin D deficiency in children with recurrent respiratory infections,
           with or without immunoglobulin deficiency
    • Authors: Nel Dąbrowska-Leonik; Ewa Bernatowska; Małgorzata Pac; Wiktor Filipiuk; Jan Mulawka; Barbara Pietrucha; Edyta Heropolitańska-Pliszka; Katarzyna Bernat-Sitarz; Beata Wolska-Kuśnierz; Bożena Mikołuć
      Pages: 173 - 178
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Nel Dąbrowska-Leonik, Ewa Bernatowska, Małgorzata Pac, Wiktor Filipiuk, Jan Mulawka, Barbara Pietrucha, Edyta Heropolitańska-Pliszka, Katarzyna Bernat-Sitarz, Beata Wolska-Kuśnierz, Bożena Mikołuć
      Purpose The objective of this study was to evaluate thevitamin D concentration in patients with recurrent respiratory infections with or without immunoglobulin G, A or M (IgG, IgA, IgM) deficiency, and to find a correlation between the vitamin D concentration and the response to hepatitis B vaccination. Materials and method The study involved 730 patients with recurrent respiratory infections. The concentration of 25-hydroxyvitamin D (25(OH)D), immunoglobulins G, A and M, anti-HBs was determined. Results The tests showed that 11% of patients presented IgG levels below the age related reference values. Children with reduced IgG concentration were also found to have significantly lower vitamin D concentrations in comparison to children with normal IgG. Vitamin D deficiency was observed in schoolchildren between 7 and 18 years of age. No correlation was found between 25(OH)D concentration and Hbs antibody levels. Conclusions An investigation of a large group of patients who have recurrent infection found patients with IgG deficiency to whom special proceeding have to be performed: 1. Significantly lower vitamin D concentration observed in the group of children with IgG deficiency implicated in long-lasting monitoring of vitamin D level require adding to the practice guidelines for Central Europe 2013. 2. Intervention treatment with suitable doses of vitamin D to clarified metabolism of vitamin D has to be plan for children with IgG deficiency and significant lower vitamin D concentration.

      PubDate: 2017-11-20T04:09:31Z
      DOI: 10.1016/j.advms.2017.08.001
       
  • Perioperative thrombocytopenia predicts poor outcome in patients
           undergoing transcatheter aortic valve implantation
    • Authors: Maciej Mitrosz; Remigiusz Kazimierczyk; Malgorzata Chlabicz; Bozena Sobkowicz; Ewa Waszkiewicz; Anna Lisowska; Slawomir Dobrzycki; Wlodzimierz J. Musial; Tomasz Hirnle; Karol A. Kaminski; Agnieszka M. Tycinska
      Pages: 179 - 184
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Maciej Mitrosz, Remigiusz Kazimierczyk, Malgorzata Chlabicz, Bozena Sobkowicz, Ewa Waszkiewicz, Anna Lisowska, Slawomir Dobrzycki, Wlodzimierz J. Musial, Tomasz Hirnle, Karol A. Kaminski, Agnieszka M. Tycinska
      Purpose To determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes. Methods Thirty two patients (mean age 78.5±7.9years, 62% females) qualified for TAVI procedure were prospectively evaluated. Platelet counts were assessed at baseline and for the next three postoperative (POD) days. Platelet activation was evaluated by P-selectin (PS, serum, ELISA) and platelet factor 4 (PF-4, CTAD plasma), and blood coagulation activation by prothrombin fragments 1+2 (F1+2, plasma, ELISA). Composite end point (CEP) including death and the need of cardiovascular rehospitalization was assessed after a mean of 14.1±6.7months. Results During the follow up period half of the patients reached CEP. Thrombocytopenia was more profound and frequent in patients with CEP as compared to those without (p<0.05). No differences regarding either the biomarkers of platelet (PS, PF-4) or coagulation (F1+F2) activation between the groups with and without CEP were found. Patients with moderate-to-severe thrombocytopenia at baseline had worse prognosis (log-rank test, p=0.0003). Based on the receiver operating characteristic curve analysis, the differences between platelet count on each postoperative day and the baseline count did not have any predictive value in CEP occurrence. Conclusions Patients with thrombocytopenia following TAVI procedure have poor prognosis, however, the changes on the particular days are not more important than initial platelet count. Further studies are needed to evaluate platelet and blood coagulation activation as potential causes of thrombocytopenia and impaired prognosis related to it.

      PubDate: 2017-11-20T04:09:31Z
      DOI: 10.1016/j.advms.2017.11.001
       
  • Human saliva as a diagnostic material
    • Authors: Sylwia Chojnowska; Tomasz Baran; Iwona Wilińska; Paulina Sienicka; Iwona Cabaj-Wiater; Małgorzata Knaś
      Pages: 185 - 191
      Abstract: Publication date: March 2018
      Source:Advances in Medical Sciences, Volume 63, Issue 1
      Author(s): Sylwia Chojnowska, Tomasz Baran, Iwona Wilińska, Paulina Sienicka, Iwona Cabaj-Wiater, Małgorzata Knaś
      Today blood biochemical laboratory tests are essential elements to the diagnosis and monitoring of the treatment of diseases. However, many researchers have suggested saliva as an preferable diagnostic material. The collection of saliva is simple, painless, cheap and safe, both for patients and medical staff. An additional advantage of saliva is the fact that it may be retrieved several times a day, which makes repeat analysis much easier. Furthermore, saliva has very high durability. Although 94–99% of salivary content is water, saliva also contains numerous cellular elements and many organic and inorganic substances, including most biological markers present in the blood and urine that may be used in the early detection and monitoring of many dental and general diseases.

      PubDate: 2017-11-20T04:09:31Z
      DOI: 10.1016/j.advms.2017.11.002
       
  • A novel germline TP53 mutation p.Pro190Arg detected in a patient with lung
           and bilateral breast cancers
    • Authors: Małgorzata Krześniak; Dorota Butkiewicz; Jadwiga Rachtan; Iwona Matuszczyk; Ewa Grzybowska; Marek Rusin
      Pages: 207 - 210
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Małgorzata Krześniak, Dorota Butkiewicz, Jadwiga Rachtan, Iwona Matuszczyk, Ewa Grzybowska, Marek Rusin
      Purpose Li-Fraumeni syndrome (LFS) is a rare genetic disease with strong predispositions to multiple early-onset neoplasms, mostly sarcomas, breast cancers, brain tumors and adrenocortical carcinomas (LFS core cancers). In most LFS families the germline mutations of TP53 tumor suppressor gene were found. Lung cancer does not belong to the core cancers of LFS, however its higher incidence is observed in families with TP53 mutations. Our aim was to search for TP53 mutations in female lung cancer patients whose clinico-demographic characteristics suggested a probable genetic predisposition to the disease. Materials and methods The coding region of TP53 from blood DNA was sequenced using Sanger method. The functioning of detected mutation was tested by luciferase reporter assay. Results We found a nucleotide substitution c.569C>G, p.Pro190Arg, which was not described in the TP53 germline mutation database (http://p53.iarc.fr/TP53GermlineMutations.aspx). The mutation destroys the ability of p53 to transactivate BAX promoter and significantly reduces transactivation potential of p53 toward the promoter of MDM2 gen. Conclusion We identified novel germline mutation of TP53.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.002
       
  • Salivary protective factors in patients suffering from decompensated type
           2 diabetes
    • Authors: Marcin Chorzewski; Karolina Orywal; Teresa Sierpinska; Maria Golebiewska
      Pages: 211 - 215
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marcin Chorzewski, Karolina Orywal, Teresa Sierpinska, Maria Golebiewska
      Purpose Defining the level of protective factors in saliva of patients suffering from decompensated type 2 diabetes. Material and methods 50 Patients with diagnosis of decompensated type 2 diabetes, including 32 women and 18 men at the age of 57.9±9.2 years. The control group consisted of 50 people among whom there were 38 women and 12 men whose average age was estimated at 51.2±9.9 years. Results It was stated the increased concentration of total protein by 60% and decreased concentration of IgA by 70%, of lysozyme by 27% and of lactoferrin by 40% in resting saliva of patients with type 2 diabetes if compared to the control group. These outcomes were really statistically meaningful. The evaluation of dependences between the analyzed protective factors and the indicator of oral cavity condition proved the positive correlation between the concentration of total protein and the number of DMFT (i.e. the rate of caries intensity). The remaining coefficients of correlation being evaluated proved to be negative and statistically meaningless. Conclusion The obtained outcomes prove a high influence of proteins included in saliva on the prevalence and development of caries at patients with decompensated type 2 diabetes.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.06.005
       
  • Genetic alterations within TLR genes in development of Toxoplasma gondii
           infection among Polish pregnant women
    • Authors: Wioletta Wujcicka; Jan Wilczyński; Dorota Nowakowska
      Pages: 216 - 222
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Wioletta Wujcicka, Jan Wilczyński, Dorota Nowakowska
      Purpose The research was conducted to evaluate the role of genotypes, haplotypes and multiple-SNP variants in the range of TLR2, TLR4 and TLR9 single nucleotide polymorphisms (SNPs) in the development of Toxoplasma gondii infection among Polish pregnant women. Material and methods The study was performed for 116 Polish pregnant women, including 51 patients infected with T. gondii, and 65 age-matched control pregnant individuals. Genotypes in TLR2 2258 G>A, TLR4 896 A>G, TLR4 1196 C>T and TLR9 2848 G>A SNPs were estimated by self-designed, nested PCR-RFLP assays. Randomly selected PCR products, representative for distinct genotypes in the studied polymorphisms, were confirmed by sequencing. All the genotypes were calculated for Hardy-Weinberg (H-W) equilibrium and TLR4 variants were tested for linkage disequilibrium. Relationships were assessed between alleles, genotypes, haplotypes or multiple-SNP variants in TLR polymorphisms and the occurrence of T. gondii infection in pregnant women, using a logistic regression model. Results All the analyzed genotypes preserved the H-W equilibrium among the studied groups of patients (P> 0.050). Similar distribution of distinct alleles and individual genotypes in TLR SNPs, as well as of haplotypes in TLR4 polymorphisms, were observed in T. gondii infected and control uninfected pregnant women. However, the GACG multiple-SNP variant, within the range of all the four studied polymorphisms, was correlated with a decreased risk of the parasitic infection (OR 0.52, 95% CI 0.28-0.97; P ≤0.050). Conclusions The polymorphisms, located within TLR2, TLR4 and TLR9 genes, may be involved together in occurrence of T. gondii infection among Polish pregnant women.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.02.002
       
  • The fact not to ignore: Mean blood pressure is the main predictor of
           
    • Authors: Alma Čypienė; Jolanta Dadonienė; Dalia Miltinienė; Egidija Rinkūnienė; Rita Rugienė; Sigita Stropuvienė; Jolita Badarienė; Aleksandras Laucevičius
      Pages: 223 - 229
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Alma Čypienė, Jolanta Dadonienė, Dalia Miltinienė, Egidija Rinkūnienė, Rita Rugienė, Sigita Stropuvienė, Jolita Badarienė, Aleksandras Laucevičius
      Purpose We aimed to evaluate the association between carotid-radial pulse wave velocity (PWV), augmentation index (AIx), and flow-mediated dilatation (FMD) of the brachial artery and factors potentially influencing them in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). Material and methods 316 patients diagnosed with RA (32%), SLE (20%), SSc (16%) and 156 controls (32%) were included in the study. Parameters of arterial stiffness AIx and PWV were obtained using applanation tonometry. FMD reflecting endothelial function was measured by ultrasound. Results AIx was increased in all three diseases (p<0.0001), but no differences were found between rheumatic diseases. In most of the RA cases PWV values were abnormal (on average by 0.52m/sec higher than in controls), while in SSc patients FMD values were diminished (p=0.006). Mean blood pressure (MBP) was the most consistent predictive factor in all three diseases, influencing both PWV and AIx, although patient age was also important in variation of AIx. The disease activity score (DAS28) was relevant only in RA patients. Furthermore, SLE disease activity index in SLE or Rodnan skin thickness score had no statistical significance in SSc and inflammatory markers. Conclusions Both, PWV and AIx are dependent on MBP and age DAS28 may affect AIx in RA patients, while other disease or inflammatory markers are unlikely to have any effect. MBP is one of the main cardiovascular risk factors affecting the arterial stiffness in RA, SLE and SSc patients therefore controlling MBP in systemic rheumatic disease patients is mandatory.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.005
       
  • Hysteroscopy in the treatment of uterine cesarean section scar
           diverticulum: A systematic review
    • Authors: Anna Abacjew-Chmylko; Dariusz G. Wydra; Hanna Olszewska
      Pages: 230 - 239
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Anna Abacjew-Chmylko, Dariusz G. Wydra, Hanna Olszewska
      The aim of this paper is to review and to analyze the results of previous studies dealing with hysteroscopic treatment of postcesarean scar defects. A systematic review of publications indexed in MEDLINE/PubMed database identified a total of 11 studies dealing with resectoscopic treatment of postcesarean scar defect. The review was conducted in line with the Meta-analysis of Observational Studies in Epidemiology (MOOSE) guidelines and the PRISMA statement. In only few studies, patients were qualified for hysteroscopic surgery based on the measurement of the defect depth and thickness of residual myometrium above the pouch. Two principal techniques were used for the hysteroscopic treatment: resection of one edge of the scar diverticulum, and resection of the inferior and superior edges of the defect. Additionally, most authors performed electrocauterization of the niche bottom. Resectoscopic treatment turned out to be highly effective in the case of women with AUB. No complications of the hysteroscopic procedure have been reported. Methodological value of the reviewed studies was relatively low due to non-unified selection/verification criteria and incomplete, non-systematic postoperative assessment. In conclusion, hysteroscopic treatment seems to be a promising option in the management of postcesarean scar defects, but still further research is needed on the problem in question.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.004
       
  • Spatial QRS-T angle in patients with newly diagnosed obstructive sleep
           apnea syndrome
    • Authors: Paweł Kiciński; Todd Schlegel; Andrzej Dybała; Maciej Zakrzewski; Sylwia Przybylska-Kuć; Wojciech Myśliński; Jerzy Mosiewicz; Stanisław Głuszek; Andrzej Jaroszyński
      Pages: 240 - 245
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Paweł Kiciński, Todd Schlegel, Andrzej Dybała, Maciej Zakrzewski, Sylwia Przybylska-Kuć, Wojciech Myśliński, Jerzy Mosiewicz, Stanisław Głuszek, Andrzej Jaroszyński
      Purpose The aim of the study was to assess the spatial QRS-T angle (QRS-TA) in a group of newly diagnosed and untreated adult patients with obstructive sleep apnea syndrome (OSAS) and to identify potential factors affecting this parameter. Patients and methods The study group (PSG-confirmed OSAS) included 62 individuals, aged 51.7±10.3 years. The control group consisted of 25 individuals, aged 46.6±16.6 years with no sleep-disordered breathing. The diagnosis of OSAS and assessment of its severity was based on unattended all-night screening polysomnography. The spatial QRS-TA was reconstructed from 12-lead ECG using Kors’ regression method. Results Significant differences of spatial QRS-TA values were found between patients with severe OSAS (36.9±18.9°) and the controls (20.3±13.4°; p <0.01) and between patients with mild or moderate OSAS (32.3±20.1°) and the controls (p =0.01). Statistically significant correlations were found between spatial QRS-TA and polysomnographic indices (i.e. AHI, AI, RDT and RDTI). Conlusions Spatial QRS-TA values are significantly higher in patients with OSAS than in controls, thus indicating increased heterogeneity of myocardial action potential. Further long-term prospective studies evaluating the prognostic value of spatial QRS-TA in OSAS patients are needed.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.11.008
       
  • Tangeretin alters neuronal apoptosis and ameliorates the severity of
           seizures in experimental epilepsy-induced rats by modulating apoptotic
           protein expressions, regulating matrix metalloproteinases, and activating
           the PI3K/Akt cell survival pathway
    • Authors: Xiao-qian Guo; Yu-ling Cao; Fang Hao; Zhong-rui Yan; Mei-ling Wang; Xue-wu Liu
      Pages: 246 - 253
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Xiao-qian Guo, Yu-ling Cao, Fang Hao, Zhong-rui Yan, Mei-ling Wang, Xue-wu Liu
      Purpose Epilepsy is complex neural disarray categorized by recurring seizures. Despite recent advances in pharmacotherapies for epilepsy, its treatment remains a challenge due to the contrary effects of the drugs. As a result, the identification of novel anti-epileptic drugs (AEDs) with neuroprotective properties and few side effects is of great value. Thus, the present study assessed the treatment effects of tangeretin using a rat model of pilocarpine-induced epilepsy. Materials and Methods Separate groups of male Wistar rats received oral administrations of tangeretin at 50, 100, or 200mg/kg for 10 days and then, on the 10th day, they received an intraperitoneal injection of pilocarpine (30mg/kg). Subsequently, neuronal degeneration and apoptosis were assessed using Nissl staining and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay procedures. Additionally, the expressions of phosphatidylinositol-3-kinase (PI3K/Akt) pathway proteins, cleaved caspase-3, Bad, Bcl-2, Bcl-xL, and Bax were determined using Western blot analyses. Results Tangeretin reduced the seizure scores and latency to first seizure of the rats and effectively activated the pilocarpine-induced suppression of PI3K/Akt signaling. Additionally, tangeretin effectively regulated the levels of apoptosis-inducing factor (AIF) in mitochondria as well as the expressions of apoptotic pathway proteins. Seizure-induced elevations in the activities and expressions of matrix metalloproteinases (MMPs)-2 and -9 were also modulated. Conclusion The present results indicate that tangeretin exerted potent neuroprotective effects against pilocarpine-induced seizures via the activation of PI3K/Akt signaling and the regulation of MMPs.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.11.011
       
  • Different manifestations of pulmonary embolism in younger compared to
           older patients: Clinical presentation, prediction rules and long-term
           outcomes
    • Authors: Izabela E. Kiluk; Agnieszka Krajewska; Urszula Kosacka; Agnieszka Tycińska; Robert Milewski; Włodzimierz Musiał; Bożena Sobkowicz
      Pages: 254 - 258
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Izabela E. Kiluk, Agnieszka Krajewska, Urszula Kosacka, Agnieszka Tycińska, Robert Milewski, Włodzimierz Musiał, Bożena Sobkowicz
      Purpose Data concerning specific manifestations of pulmonary embolism (PE) among younger patients are scarce. We aimed to evaluate differences in clinical presentation, PE prediction rules, thrombolytic treatment use and PE outcomes in younger (<50 years of age) compared to older patients. Material/methods We studied 238 consecutive patients with proven PE who were retrospectively categorized into three PE probability subgroups according to the revised Geneva score (RGS) and Wells score (WS). Duration of follow-up was up to 115 months. Results Younger patients accounted for 19.7% of the study cohort. Obesity and smoking were significantly more common, while comorbidities were less common (P <0.05) in the younger patients. According to RGS and WS, younger patients were more often categorized into the low PE probability subgroup and rarely into the high probability subgroup (P <0.05). We found no differences in clinical signs, symptoms, and treatment between the two groups. In-hospital (2% vs. 13%) and long-term (12% vs. 36%) mortality rates were significantly lower in younger patients (P =0.003). Conclusions In younger PE patients, despite differences in predisposing factors and PE probability grading as assessed by RGS and WS, clinical features at admission and treatment were similar compared to the older group. Our findings confirmed lower mortality among younger compared to older patients.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.001
       
  • Osteogenic differentiation of mesenchymal stem cells is impaired by bone
           morphogenetic protein 7
    • Authors: Paweena Wongwitwichot; Jasadee Kaewsrichan
      Pages: 266 - 272
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Paweena Wongwitwichot, Jasadee Kaewsrichan
      Purpose Mesenchymal stem cells (MSCs) are multipotent adult stem cells and present in practically all tissues but originally identified within the bone marrow (BM). The differentiation potential of these cells is generally impaired when culturing in vitro for cell expansion. The aim of this study is to speedily increase the numbers of bone marrow derived mesenchymal stem cells (BM-MSCs) with substantially maintaining their differentiation potential in vitro and improving bone formation in vivo. Materials and methods BM-MSCs isolated from rats were sequentially cultured in α-MEM containing basic fibroblast growth factor (FGF2) and/or insulin to stimulate proliferation and osteogenic commitment, and in the medium with the addition of bone morphogenetic protein 2 (BMP2) and/or bone morphogenetic protein 7 (BMP7) to arouse differentiation. The expression of genes markedly associating the commitment and differentiation were investigated in vitro using real-time PCR technique and mineralization assay, while the capacity of inducing bone formation by the established conditions was determined in vivo using a rat model. Results The BM-MSCs greatly proliferated with active transcription of runx2 and osterix genes when induced by FGF2 and insulin. The in vitro mineralization was enhanced by BMP2, but the extent was diminished when BMP2 was replaced or supplemented by BMP7. Formation of new small blood vessels was notably detected when the cells were respectively challenged by FGF2 plus insulin and BMP2. Conclusion These data are valuable in choosing growth factors for proper bone repair. However, optimization of the established system would be essential when the cells of human source are applied.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.001
       
  • Treatment of refractory epilepsy patients with autologous mesenchymal stem
           cells reduces seizure frequency: An open label study
    • Authors: Fedor Hlebokazov; Tatiana Dakukina; Svetlana Ihnatsenko; Svetlana Kosmacheva; Michael Potapnev; Antos Shakhbazau; Natalia Goncharova; Michael Makhrov; Pavel Korolevich; Nikolai Misyuk; Victoria Dakukina; Irina Shamruk; Elena Slobina; Sergei Marchuk
      Pages: 273 - 279
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Fedor Hlebokazov, Tatiana Dakukina, Svetlana Ihnatsenko, Svetlana Kosmacheva, Michael Potapnev, Antos Shakhbazau, Natalia Goncharova, Michael Makhrov, Pavel Korolevich, Nikolai Misyuk, Victoria Dakukina, Irina Shamruk, Elena Slobina, Sergei Marchuk
      Purpose Existing anti-epileptic drugs (AED) have limited efficiency in many patients, necessitating the search for alternative approaches such as stem cell therapy. We report the use of autologous patient-derived mesenchymal stem cells (MSC) as a therapeutic agent in symptomatic drug-resistant epilepsy in a Phase I open label clinical trial (registered as NCT02497443). Patients and methods The patients received either standard treatment with AED (control group), or AED supplemented with single intravenous administration of undifferentiated autologous MSC (target dose of 1×106 cells/kg), followed by a single intrathecal injection of neurally induced autologous MSC (target dose of 0.1×106 cells/kg). Results MSC injections were well tolerated and did not cause any severe adverse effects. Seizure frequency was designated as the main outcome and evaluated at 1 year time point. 3 out of 10 patients in MSC therapy group achieved remission (no seizures for one year and more), and 5 additional patients became responders to AEDs, while only 2 out of 12 patients became responders in control group (difference significant, P =0.0135). Conclusions MSC possess unique immunomodulatory properties and are a safe and promising candidate for cell therapy in AED resistant epilepsy patients.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.004
       
  • Methods to evaluate arterial structure and function in children –
           State-of-the art knowledge
    • Authors: Piotr Skrzypczyk; Małgorzata Pańczyk-Tomaszewska
      Pages: 280 - 294
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Piotr Skrzypczyk, Małgorzata Pańczyk-Tomaszewska
      Background With increasing rates of hypertension, obesity, and diabetes in the pediatric population, wide available, and reproducible methods are necessary to evaluate arterial structure and function in children and adolescents. Methods MEDLINE/Pubmed was searched for articles published in years 2012–2017 on methodology of, current knowledge on, and limitations of the most commonly used methods to evaluate central, proximal and coronary arteries, as well as endothelial function in pediatric patients. Results Among 1528 records screened (including 1475 records from years 2012 to 2017) 139 papers were found suitable for the review. Following methods were discussed in this review article: ultrasound measurements of the intima-media thickness, coronary calcium scoring using computed tomography, arterial stiffness measurements (pulse wave velocity and pulse wave analysis, carotid artery distensibility, pulse pressure, and ambulatory arterial stiffness index), ankle-brachial index, and methods to evaluate vascular endothelial function (flow-mediated vasodilation, peripheral arterial tonometry, Doppler laser flowmetry, and cellular and soluble markers of endothelial dysfunction). Conclusions Ultrasonographic measurement of carotid intima-media thickness and measurement of pulse wave velocity (by oscillometry or applanation tonometry) are highly reproducible methods applicable for both research and clinical practice with proved applicability for children aged ≥6 years or with height ≥120cm. Evaluation of ambulatory arterial stiffness index by ambulatory blood pressure monitoring is another promising option in pediatric high-risk patients. Clearly, further studies are necessary to evaluate usefulness of these and other methods for the detection of subclinical arterial damage in children.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.03.001
       
  • Effects of fine air particulates on gene expression in non-small-cell lung
           cancer
    • Authors: Biao Yang; Xinming Li; Dongmei Chen; Chunling Xiao
      Pages: 295 - 301
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Biao Yang, Xinming Li, Dongmei Chen, Chunling Xiao
      Purpose Airborne particulate matter smaller than 2.5μm (PM2.5) has been shown to induce adverse health effects through various mechanisms. However, its effects on gene expression in non-small-cell lung cancer (NSCLC) remain undefined. The aim of this study was to analyze the expression profile of PM2.5-induced adverse health effects on human. Materials and methods We performed RNA sequencing to elucidate key molecular effects of PM2.5 collected from Shenyang China, to identify potential diagnostic markers or therapeutic targets, and further validated these differences in gene expression by using quantitative PCR in A549 and H1299 human non-small-cell lung cancer cell lines. To investigate the functional changes on PM2.5 exposed cells, we carried out the viability assay for the cell counting, and the Boyden chamber assay for invasion. Results We found 143 genes that were expressed at least twice as much, or no more than half as much, in NSCLC cells exposed to PM2.5 than in unexposed cells. Results showed deregulated genes confronted PM2.5 exposure were significantly expressed, but commonly expressed in NSCLC cells. In addition, according to the viability assay and the Boyden chamber assay, PM2.5 exposed cells which have more competent on proliferation and invasion can keep the line with the results in RNA-Seq. Conclusion Our data may provide a more specific understanding of the signaling patterns associated with pathogenesis, and lead to novel markers and therapeutic targets for NSCLC.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.003
       
  • Mulberry leaf extract decreases digestion and absorption of starch in
           healthy subjects—A randomized, placebo-controlled, crossover study
    • Authors: Jan Józefczuk; Klaudia Malikowska; Aleksandra Glapa; Barbara Stawińska-Witoszyńska; Jan Krzysztof Nowak; Joanna Bajerska; Aleksandra Lisowska; Jarosław Walkowiak
      Pages: 302 - 306
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Jan Józefczuk, Klaudia Malikowska, Aleksandra Glapa, Barbara Stawińska-Witoszyńska, Jan Krzysztof Nowak, Joanna Bajerska, Aleksandra Lisowska, Jarosław Walkowiak
      Purpose Mulberry (Morus alba L.) leaf tea has recently received much attention as a dietary supplement due to the wide range of putative health benefits, such as antidiabetic effects. Nevertheless, data evaluating its influence on carbohydrate metabolism in humans are scarce. The present study aims to investigate the effect of mulberry leaf extract supplementation on starch digestion and absorption in humans. Materials and methods The study comprised of 25 healthy subjects, aged 19–27 years. In all subjects, a starch 13C breath test was performed twice in a crossover and single blind design. Subjects were initially randomized to ingest naturally 13C-abundant cornflakes (50g cornflakes+100ml low fat milk) either with the mulberry leaf extract (36mg of active component-1-deoxynojirimycin) or the placebo and each subject received the opposite preparation one week later. Results The cumulative percentage dose recovery was lower for the mulberry leaf extract test than for the placebo test (median [quartile distribution]: 13.9% [9.9–17.4] vs. 17.2% [13.3–20.6]; p=0.015). A significant decrease was detectable from minute 120 after the ingestion. Conclusions A single dose of mulberry leaf extract taken with a test meal decreases starch digestion and absorption. These findings could possibly be translated into everyday practice for improvement of postprandial glycemic control.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.03.002
       
  • Ischaemic preconditioning – Current knowledge and potential future
           applications after 30 years of experience
    • Authors: Karolina Stokfisz; Anna Ledakowicz-Polak; Maciej Zagorski; Marzenna Zielinska
      Pages: 307 - 316
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Karolina Stokfisz, Anna Ledakowicz-Polak, Maciej Zagorski, Marzenna Zielinska
      Ischaemic preconditioning (IPC) phenomenon has been known for thirty years. During that time several studies showed that IPC provided by brief ischaemic and reperfusion episodes prior to longer ischaemia can bestow a protective effect to both preconditioned and also remote organs. IPC affecting remote organs is called remote ischaemic preconditioning. Initially, most IPC studies were focused on enhancing myocardial resistance to subsequent ischaemia and reperfusion injury. However, preconditioning was found to be a universal phenomenon and was observed in various organs and tissues including the heart, liver, brain, retina, kidney, skeletal muscles and intestine. Currently, there are a lot of simultaneous studies are underway aiming at finding out whether IPC can be helpful in protecting these organs. The mechanism of local and remote IPC is complex and not well known. Several triggers, intracellular pathways and effectors, humoral, neural and induced by genetic changes may be considered potential pathways in the protective activity of local and remote IPC. Local and remote IPC mechanism may potentially serve as heart protection during cardiac surgery and may limit the infarct size of the myocardium, can be a strategy for preventing the development of acute kidney injury development and liver damage during transplantation, may protect the brain against ischaemic injury. In addition, the method is safe, non-invasive, cheap and easily applicable. The main purpose of this review article is to present new advances which would help to understand the potential mechanism of IPC. It also discusses both its potential applications and utility in clinical settings.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.006
       
  • Impact of exaggerated blood pressure response in normotensive individuals
           on future hypertension and prognosis: Systematic review according to
           PRISMA guideline
    • Authors: Karsten Keller; Kathrin Stelzer; Mir Abolfazl Ostad; Felix Post
      Pages: 317 - 329
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Karsten Keller, Kathrin Stelzer, Mir Abolfazl Ostad, Felix Post
      Purpose Arterial hypertension (aHT) is the leading risk factor for morbidity and mortality worldwide. Blood pressure (BP) deviation at rest is well defined and accompanies risk for cardiovascular events and cardiovascular mortality. A growing body of evidence emphasises that an exaggerated blood pressure response (EBPR) in cardiopulmonary exercise testing (CPET) could help to identify seemingly cardiovascular healthy and normotensive subjects, who have an increased risk of developing aHT and cardiovascular events in the future. Materials and methods The PubMed online database was searched for published studies reporting exercise-related BP and both the risk of aHT and cardiovascular events in the future. Results We identified 18 original studies about EBPR in CPET, which included a total of 35,151 normotensive individuals for prediction of new onset of aHT in the future and 11 original studies with 43,012 enrolled subjects with the endpoint of cardiovascular events in the future. Although an EBPR under CPET is not well defined, a large number of studies emphasise that EBPR in CPET is associated with both new-onset aHT and cardiovascular events in the future. Conclusions A growing number of studies support the hypothesis that EBPR in CPET may be a diagnostic tool to identify subjects with an elevated risk of developing aHT and cardiovascular events in the future.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.010
       
  • Effects of γ-radiation on cell growth, cell cycle and promoter
           methylation of 22 cell cycle genes in the 1321NI astrocytoma cell line
    • Authors: Yaman Alghamian; Ghalia Abou Alchamat; Hossam Murad; Ammar Madania
      Pages: 330 - 337
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Yaman Alghamian, Ghalia Abou Alchamat, Hossam Murad, Ammar Madania
      Purpose DNA damage caused by radiation initiates biological responses affecting cell fate. DNA methylation regulates gene expression and modulates DNA damage pathways. Alterations in the methylation profiles of cell cycle regulating genes may control cell response to radiation. In this study we investigated the effect of ionizing radiation on the methylation levels of 22 cell cycle regulating genes in correlation with gene expression in 1321NI astrocytoma cell line. Methods 1321NI cells were irradiated with 2, 5 or 10Gy doses then analyzed after 24, 48 and 72h for cell viability using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu bromide) assay. Flow cytometry were used to study the effect of 10Gy irradiation on cell cycle. EpiTect Methyl II PCR Array was used to identify differentially methylated genes in irradiated cells. Changes in gene expression was determined by qPCR. Azacytidine treatment was used to determine whether DNA methylation affectes gene expression. Results Our results showed that irradiation decreased cell viability and caused cell cycle arrest at G2/M. Out of 22 genes tested, only CCNF and RAD9A showed some increase in DNA methylation (3.59% and 3.62%, respectively) after 10Gy irradiation, and this increase coincided with downregulation of both genes (by 4 and 2 fold, respectively). Treatment with azacytidine confirmed that expression of CCNF and RAD9A genes was regulated by methylation. Conclusions 1321NI cell line is highly radioresistant and that irradiation of these cells with a 10Gy dose increases DNA methylation of CCNF and RAD9A genes. This dose down-regulates these genes, favoring G2/M arrest.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2017.03.004
       
  • Pro-inflammatory cytokines associated with clinical severity of dry eye
           disease of patients with depression
    • Authors: Małgorzata Mrugacz; Lucyna Ostrowska; Anna Bryl; Agata Szulc; Beata Zelazowska-Rutkowska; Grzegorz Mrugacz
      Pages: 338 - 344
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Małgorzata Mrugacz, Lucyna Ostrowska, Anna Bryl, Agata Szulc, Beata Zelazowska-Rutkowska, Grzegorz Mrugacz
      Purpose The aim of this study was to assess the correlation of inflammatory cytokines levels in tears with severity of dry eye disease in a cohort of patients with depression. Material and methods Tear fluid samples were collected from 32 patients with depression treated with antidepressants, and 34 healthy subjects. Cytokines were assessed by ELISA. All the subjects completed the Beck Depression Inventory and performed the ophthalmic examination, including dry eye tests. Results The tear fluid levels of IL-6, IL-17 and TNF-α in depressive patients were higher than in controls. The clinical severity of dry eye disease correlated significantly with the IL-17 and TNF-α levels. Conclusions Our results suggest a crucial role of inflammatory cytokines, especially IL-17 and TNF-α, in the development of severe dry eye disease in patients with depression. Clarification of the role pro-inflammatory cytokines in the pathogenesis of ocular findings in depressive patients may be useful in establishing immunotherapeutic strategies for this disease.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2017.03.003
       
  • Bacterial infections and hepatic encephalopathy in liver
           cirrhosis–prophylaxis and treatment
    • Authors: Damian Piotrowski; Anna Boroń-Kaczmarska
      Pages: 345 - 356
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Damian Piotrowski, Anna Boroń-Kaczmarska
      Infections are common among patients with liver cirrhosis. They occur more often in cirrhotic patient groups than in the general population and result in higher mortality. One reason for this phenomenon is bacterial translocation from the intestinal lumen that occurs as a consequence of intestinal bacterial overgrowth, increased permeability and decreased motility. The most common infections in cirrhotic patients are spontaneous bacterial peritonitis and urinary tract infections, followed by pneumonia, skin and soft tissue infections. Intestinal bacterial overgrowth is also responsible for hyperammonemia, which leads to hepatic encephalopathy. All of these complications make this group of patients at high risk for mortality. The role of antibiotics in liver cirrhosis is to treat and in some cases to prevent the development of infectious complications. Based on our current knowledge, antibiotic prophylaxis should be administered to patients with gastrointestinal hemorrhage, low ascitic fluid protein concentration combined with liver or renal failure, and spontaneous bacterial peritonitis as a secondary prophylaxis, as well as after hepatic encephalopathy episodes (also as a secondary prophylaxis). In some cases, the use of non-antibiotic prophylaxis can also be considered. Current knowledge of the treatment of infections allows the choice of a preferred antibiotic for empiric therapy depending on the infection location and whether the source of the disease is nosocomial or community-acquired.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.009
       
  • Clinical usefulness of videocapillaroscopy and selected endothelial cell
           activation markers in people with Type 1 diabetes mellitus complicated by
           microangiopathy
    • Authors: Anna Kuryliszyn-Moskal; Wieslaw Zarzycki; Artur Dubicki; Diana Moskal; Bozena Kosztyła-Hojna; Anna Hryniewicz
      Pages: 368 - 373
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Anna Kuryliszyn-Moskal, Wieslaw Zarzycki, Artur Dubicki, Diana Moskal, Bozena Kosztyła-Hojna, Anna Hryniewicz
      Purpose Microvascular abnormalities are one of the most important causes of persistent diabetic complications. The aim of our study was to compare microvascular changes examined by nailfold videocapillaroscopy (NVC) examination with serum concentrations of vascular endothelial growth factor (VEGF), soluble thrombomodulin (sTM) and endothelin-1 (ET-1) in people with Type 1 diabetes with and without microangiopathy. Material/methods The study included 106 people with Type 1 diabetes and 40 healthy controls. All participants were evaluated by extensive clinical, laboratory and capillaroscopic studies. NVC was performed using a stereomicroscope SZ 4045 (Olympus, Germany). The intensity of morphological changes was graded from 0 to 3. Serum levels of VEGF, sTM and ET-1 were determined by an enzyme-linked immunosorbent assay (ELISA). Results Morphological changes were observed by NVC in 86 out of 106 (81%) people with Type 1 diabetes mellitus. Severe capillaroscopic changes (score 3) were seen in 32 out of 54 (59%) people with microangiopathy, but in only seven out of 52 (13%) individuals without microangiopathy. Higher serum concentration of VEGF (p <0.001), ET-1 (p <0.001) and sTM (p <0.05) were demonstrated in people with diabetes complicated with microangiopathy compared to healthy controls. Moreover, comparison between people with and without microangiopathic complications showed a significantly higher capillaroscopic score and sTM serum concentration in the group with retinopathy (p <0.001) nephropathy (p <0.001) and neuropathy (p <0.01). Conclusions Our results suggest that abnormalities in NVC may reflect the extent of microvascular involvement and associated with higher VEGF, sTM and ET-1 serum levels, as well as with microangiopathic complications in diabetic people.

      PubDate: 2017-05-23T08:59:49Z
      DOI: 10.1016/j.advms.2016.11.007
       
  • Thrombocytopenia associated with TAVI—The summary of possible causes
    • Authors: Maciej Mitrosz; Malgorzata Chlabicz; Katarzyna Hapaniuk; Karol A. Kaminski; Bozena Sobkowicz; Jaroslaw Piszcz; Slawomir Dobrzycki; Wlodzimierz J. Musial; Tomasz Hirnle; Agnieszka M. Tycinska
      Pages: 378 - 382
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Maciej Mitrosz, Malgorzata Chlabicz, Katarzyna Hapaniuk, Karol A. Kaminski, Bozena Sobkowicz, Jaroslaw Piszcz, Slawomir Dobrzycki, Wlodzimierz J. Musial, Tomasz Hirnle, Agnieszka M. Tycinska
      Thrombocytopenia (TP) following transcatheter aortic valve implantation (TAVI) procedure is a common phenomenon but the underlying mechanisms are neither well known nor described. Postinterventional severe TP is related to worse early and late outcome. Moreover, the statement of enhanced platelet and coagulation activation might justify even stronger antiplatelet and anticoagulation therapy following TAVI procedure. Thus, the examination of the pathomechanisms responsible for TP post TAVI seems to be crucial. Several hypotheses have been raised. TP can be caused by insufficient production or impaired platelet renewal. On the other hand, increased platelet activation, consumption and destruction might also be responsible for TP. These findings, mostly related to the procedure alone, need further investigation. Here, we summarize the potential multifactorial causes of post TAVI thrombocytopenia.

      PubDate: 2017-05-28T11:16:38Z
      DOI: 10.1016/j.advms.2017.04.003
       
  • The human microbiome
    • Authors: Hubert E. Blum
      Pages: 414 - 420
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Hubert E. Blum
      Until recently, human microbiology was based on the identification of single microbes, such as bacteria, fungi and viruses, frequently isolated from patients with acute or chronic infections. Novel culture-independent molecular biochemical analyses (genomics, transcriptomics, proteomics, metabolomics) allow today to detect and classify the diverse microorganisms in a given ecosystem (microbiota), such as the gastrointestinal tract, the skin, the airway system, the urogenital tract and others, and to assess all genomes in these ecosystems (microbiome) as well as their gene products. These analyses revealed that each individual has its own microbiota that plays a role in health and disease. In addition, they greatly contributed to the recent advances in the understanding of the pathogenesis of a wide range of human diseases. It is to be expected that these new insights will translate into diagnostic, therapeutic and preventive measures in the context of personalized/precision medicine.

      PubDate: 2017-07-21T07:18:26Z
      DOI: 10.1016/j.advms.2017.04.005
       
  • A device for testing the durability and exploitation reliability of dental
           prostheses
    • Authors: Marek
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marek Jałbrzykowski
      Purpose The aim of this work was to develop the design of the construction of a stand for testing the operational stability of dental prostheses, designed on the basis of an own idea. There are no devices that would make it possible to test complete structures, including the actual kinematics of motion and the influence of the environment of the oral cavity. Materials and methods Presented were several devices used for testing both fixed and removable prostheses. Attention was paid to their limited possibilities in the area of the tested friction node, kinematics of motion, and the assessment of the influence of the environment of the oral cavity on the tested functional quality. The construction was based on the author's own ideas as well as on a review of the available literature. Results As the final result of the research, simplified images of the stand were presented along with their descriptions. Conclusions The experimental verification of the stand allowed concluding that the research aims have been achieved. First of all, the device allows testing prosthetic structures of different sizes and it is possible to load the tested prostheses in randomly chosen points.

      PubDate: 2017-05-13T07:03:55Z
       
  • CS-PEG decorated PLGA nano-prototype for delivery of bioactive compounds:
           A novel approach for induction of apoptosis in HepG2 cell line
    • Authors: Ahmed A. Abd-Rabou; Hanaa H. Ahmed
      Abstract: Publication date: Available online 12 March 2017
      Source:Advances in Medical Sciences
      Author(s): Ahmed A. Abd-Rabou, Hanaa H. Ahmed
      Purpose Polymer-based nanoparticles are used as vectors for cancer drug delivery. The bioactive compounds (quercetin, ellagic acid and gallic acid) are well known to be not only antioxidants but also chemopreventive candidates against various types of cancers. To circumvent the low bioavailability and the short half-life time obstacles, we hypothesized a novel PLGA nano-platform functionalized with CS and PEG to encapsulate these phytochemicals. This encapsulation will protect the compounds from the phagocytic uptake and deliver PLGA-CS-PEG nano-prototype with high biodegradability and biosafety. Materials and methods Three consequent types of PLGA-based nanocomposites were prepared and characterized. Furthermore, we investigated the newly synthesized nano-formulations against human hepatocellular carcinoma (HepG2) and colorectal cancer (HCT 116) cell lines using cell growth inhibition assays, followed by apoptosis and necrosis assays using flow cytometry to detect the underlying mechanism of HepG2 cell death. Results Through Malvern Zeta Sizer, we recorded that the average diameters of the nano-prototypes ranged from 150 to 300nm. Regarding the cytotoxic activity of quercetin, ellagic acid, and gallic acid-encapsulated PLGA, PLGA-CS, and PLGA-CS-PEG nano-prototypes has been found that they reduce the IC50s of the HepG2 cells values by 2.2, 2.9, 2.8-folds, 1, 1.5, 2.7-folds, and 0.9, 0.7, 1.5-folds, respectively. Mechanistically, the nano-platforms of quercetin seem to be dependent on both apoptosis and necrosis, while those of ellagic acid and gallic acid are mainly dependent on apoptosis. Conclusions CS-PEG-blended PLGA nano-delivery system of quercetin, ellagic acid and gallic acid can potentiate apoptosis-mediated cell death in HepG2 cell line.
      Graphical abstract image

      PubDate: 2017-03-16T17:39:48Z
      DOI: 10.1016/j.advms.2017.01.003
       
 
 
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