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Publisher: Elsevier   (Total: 3042 journals)

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Showing 1 - 200 of 3042 Journals sorted alphabetically
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 20, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 17, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 81, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 23, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 27, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 328, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription  
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 206, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 9, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 23, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 4, SJR: 0.383, h-index: 19)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 2)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 3)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 5)
Additive Manufacturing     Hybrid Journal   (Followers: 7, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 20)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 128, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 16, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 25, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 10, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 20, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 25, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 2.139, h-index: 42)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 24, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 10, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 28, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 4)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 40, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 25, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 40, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 48, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 15)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 12)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 21, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 25)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 35, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 4)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 9)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 25, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 15, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 7, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 19, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 59)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 2, SJR: 0.1, h-index: 2)
Advances in Space Research     Full-text available via subscription   (Followers: 340, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 6, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 30, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 15)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 43, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 309, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 5, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 8, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 400, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 30, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 38, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 50, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 9, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 6)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access  
Algal Research     Partially Free   (Followers: 8, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 7, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 48, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 5)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 3)
American Heart J.     Hybrid Journal   (Followers: 48, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 44, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 36, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 6, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 16, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 30, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 24, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 34, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 46, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 179, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 55, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 2)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 23, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 24, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 21, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 33, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 55, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 9)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 2, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 38, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 160, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 11)
Anesthésie & Réanimation     Full-text available via subscription  
Anesthesiology Clinics     Full-text available via subscription   (Followers: 22, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 153, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)
Animal Reproduction Science     Hybrid Journal   (Followers: 5, SJR: 0.711, h-index: 78)
Annales d'Endocrinologie     Full-text available via subscription   (SJR: 0.394, h-index: 30)
Annales d'Urologie     Full-text available via subscription  
Annales de Cardiologie et d'Angéiologie     Full-text available via subscription   (SJR: 0.177, h-index: 13)
Annales de Chirurgie de la Main et du Membre Supérieur     Full-text available via subscription  
Annales de Chirurgie Plastique Esthétique     Full-text available via subscription   (Followers: 2, SJR: 0.354, h-index: 22)
Annales de Chirurgie Vasculaire     Full-text available via subscription   (Followers: 1)

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Journal Cover Advances in Medical Sciences
  [SJR: 0.489]   [H-I: 25]   [6 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1896-1126 - ISSN (Online) 1898-4002
   Published by Elsevier Homepage  [3042 journals]
  • A novel germline TP53 mutation p.Pro190Arg detected in a patient with lung
           and bilateral breast cancers
    • Authors: Małgorzata Krześniak; Dorota Butkiewicz; Jadwiga Rachtan; Iwona Matuszczyk; Ewa Grzybowska; Marek Rusin
      Pages: 207 - 210
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Małgorzata Krześniak, Dorota Butkiewicz, Jadwiga Rachtan, Iwona Matuszczyk, Ewa Grzybowska, Marek Rusin
      Purpose Li-Fraumeni syndrome (LFS) is a rare genetic disease with strong predispositions to multiple early-onset neoplasms, mostly sarcomas, breast cancers, brain tumors and adrenocortical carcinomas (LFS core cancers). In most LFS families the germline mutations of TP53 tumor suppressor gene were found. Lung cancer does not belong to the core cancers of LFS, however its higher incidence is observed in families with TP53 mutations. Our aim was to search for TP53 mutations in female lung cancer patients whose clinico-demographic characteristics suggested a probable genetic predisposition to the disease. Materials and methods The coding region of TP53 from blood DNA was sequenced using Sanger method. The functioning of detected mutation was tested by luciferase reporter assay. Results We found a nucleotide substitution c.569C>G, p.Pro190Arg, which was not described in the TP53 germline mutation database (http://p53.iarc.fr/TP53GermlineMutations.aspx). The mutation destroys the ability of p53 to transactivate BAX promoter and significantly reduces transactivation potential of p53 toward the promoter of MDM2 gen. Conclusion We identified novel germline mutation of TP53.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.002
       
  • Salivary protective factors in patients suffering from decompensated type
           2 diabetes
    • Authors: Marcin Chorzewski; Karolina Orywal; Teresa Sierpinska; Maria Golebiewska
      Pages: 211 - 215
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marcin Chorzewski, Karolina Orywal, Teresa Sierpinska, Maria Golebiewska
      Purpose Defining the level of protective factors in saliva of patients suffering from decompensated type 2 diabetes. Material and methods 50 Patients with diagnosis of decompensated type 2 diabetes, including 32 women and 18 men at the age of 57.9±9.2 years. The control group consisted of 50 people among whom there were 38 women and 12 men whose average age was estimated at 51.2±9.9 years. Results It was stated the increased concentration of total protein by 60% and decreased concentration of IgA by 70%, of lysozyme by 27% and of lactoferrin by 40% in resting saliva of patients with type 2 diabetes if compared to the control group. These outcomes were really statistically meaningful. The evaluation of dependences between the analyzed protective factors and the indicator of oral cavity condition proved the positive correlation between the concentration of total protein and the number of DMFT (i.e. the rate of caries intensity). The remaining coefficients of correlation being evaluated proved to be negative and statistically meaningless. Conclusion The obtained outcomes prove a high influence of proteins included in saliva on the prevalence and development of caries at patients with decompensated type 2 diabetes.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.06.005
       
  • Genetic alterations within TLR genes in development of Toxoplasma gondii
           infection among Polish pregnant women
    • Authors: Wioletta Wujcicka; Jan Wilczyński; Dorota Nowakowska
      Pages: 216 - 222
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Wioletta Wujcicka, Jan Wilczyński, Dorota Nowakowska
      Purpose The research was conducted to evaluate the role of genotypes, haplotypes and multiple-SNP variants in the range of TLR2, TLR4 and TLR9 single nucleotide polymorphisms (SNPs) in the development of Toxoplasma gondii infection among Polish pregnant women. Material and methods The study was performed for 116 Polish pregnant women, including 51 patients infected with T. gondii, and 65 age-matched control pregnant individuals. Genotypes in TLR2 2258 G>A, TLR4 896 A>G, TLR4 1196 C>T and TLR9 2848 G>A SNPs were estimated by self-designed, nested PCR-RFLP assays. Randomly selected PCR products, representative for distinct genotypes in the studied polymorphisms, were confirmed by sequencing. All the genotypes were calculated for Hardy-Weinberg (H-W) equilibrium and TLR4 variants were tested for linkage disequilibrium. Relationships were assessed between alleles, genotypes, haplotypes or multiple-SNP variants in TLR polymorphisms and the occurrence of T. gondii infection in pregnant women, using a logistic regression model. Results All the analyzed genotypes preserved the H-W equilibrium among the studied groups of patients (P> 0.050). Similar distribution of distinct alleles and individual genotypes in TLR SNPs, as well as of haplotypes in TLR4 polymorphisms, were observed in T. gondii infected and control uninfected pregnant women. However, the GACG multiple-SNP variant, within the range of all the four studied polymorphisms, was correlated with a decreased risk of the parasitic infection (OR 0.52, 95% CI 0.28-0.97; P ≤0.050). Conclusions The polymorphisms, located within TLR2, TLR4 and TLR9 genes, may be involved together in occurrence of T. gondii infection among Polish pregnant women.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.02.002
       
  • The fact not to ignore: Mean blood pressure is the main predictor of
           
    • Authors: Alma Čypienė; Jolanta Dadonienė; Dalia Miltinienė; Egidija Rinkūnienė; Rita Rugienė; Sigita Stropuvienė; Jolita Badarienė; Aleksandras Laucevičius
      Pages: 223 - 229
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Alma Čypienė, Jolanta Dadonienė, Dalia Miltinienė, Egidija Rinkūnienė, Rita Rugienė, Sigita Stropuvienė, Jolita Badarienė, Aleksandras Laucevičius
      Purpose We aimed to evaluate the association between carotid-radial pulse wave velocity (PWV), augmentation index (AIx), and flow-mediated dilatation (FMD) of the brachial artery and factors potentially influencing them in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). Material and methods 316 patients diagnosed with RA (32%), SLE (20%), SSc (16%) and 156 controls (32%) were included in the study. Parameters of arterial stiffness AIx and PWV were obtained using applanation tonometry. FMD reflecting endothelial function was measured by ultrasound. Results AIx was increased in all three diseases (p<0.0001), but no differences were found between rheumatic diseases. In most of the RA cases PWV values were abnormal (on average by 0.52m/sec higher than in controls), while in SSc patients FMD values were diminished (p=0.006). Mean blood pressure (MBP) was the most consistent predictive factor in all three diseases, influencing both PWV and AIx, although patient age was also important in variation of AIx. The disease activity score (DAS28) was relevant only in RA patients. Furthermore, SLE disease activity index in SLE or Rodnan skin thickness score had no statistical significance in SSc and inflammatory markers. Conclusions Both, PWV and AIx are dependent on MBP and age DAS28 may affect AIx in RA patients, while other disease or inflammatory markers are unlikely to have any effect. MBP is one of the main cardiovascular risk factors affecting the arterial stiffness in RA, SLE and SSc patients therefore controlling MBP in systemic rheumatic disease patients is mandatory.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.005
       
  • Hysteroscopy in the treatment of uterine cesarean section scar
           diverticulum: A systematic review
    • Authors: Anna Abacjew-Chmylko; Dariusz G. Wydra; Hanna Olszewska
      Pages: 230 - 239
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Anna Abacjew-Chmylko, Dariusz G. Wydra, Hanna Olszewska
      The aim of this paper is to review and to analyze the results of previous studies dealing with hysteroscopic treatment of postcesarean scar defects. A systematic review of publications indexed in MEDLINE/PubMed database identified a total of 11 studies dealing with resectoscopic treatment of postcesarean scar defect. The review was conducted in line with the Meta-analysis of Observational Studies in Epidemiology (MOOSE) guidelines and the PRISMA statement. In only few studies, patients were qualified for hysteroscopic surgery based on the measurement of the defect depth and thickness of residual myometrium above the pouch. Two principal techniques were used for the hysteroscopic treatment: resection of one edge of the scar diverticulum, and resection of the inferior and superior edges of the defect. Additionally, most authors performed electrocauterization of the niche bottom. Resectoscopic treatment turned out to be highly effective in the case of women with AUB. No complications of the hysteroscopic procedure have been reported. Methodological value of the reviewed studies was relatively low due to non-unified selection/verification criteria and incomplete, non-systematic postoperative assessment. In conclusion, hysteroscopic treatment seems to be a promising option in the management of postcesarean scar defects, but still further research is needed on the problem in question.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.004
       
  • Spatial QRS-T angle in patients with newly diagnosed obstructive sleep
           apnea syndrome
    • Authors: Paweł Kiciński; Todd Schlegel; Andrzej Dybała; Maciej Zakrzewski; Sylwia Przybylska-Kuć; Wojciech Myśliński; Jerzy Mosiewicz; Stanisław Głuszek; Andrzej Jaroszyński
      Pages: 240 - 245
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Paweł Kiciński, Todd Schlegel, Andrzej Dybała, Maciej Zakrzewski, Sylwia Przybylska-Kuć, Wojciech Myśliński, Jerzy Mosiewicz, Stanisław Głuszek, Andrzej Jaroszyński
      Purpose The aim of the study was to assess the spatial QRS-T angle (QRS-TA) in a group of newly diagnosed and untreated adult patients with obstructive sleep apnea syndrome (OSAS) and to identify potential factors affecting this parameter. Patients and methods The study group (PSG-confirmed OSAS) included 62 individuals, aged 51.7±10.3 years. The control group consisted of 25 individuals, aged 46.6±16.6 years with no sleep-disordered breathing. The diagnosis of OSAS and assessment of its severity was based on unattended all-night screening polysomnography. The spatial QRS-TA was reconstructed from 12-lead ECG using Kors’ regression method. Results Significant differences of spatial QRS-TA values were found between patients with severe OSAS (36.9±18.9°) and the controls (20.3±13.4°; p <0.01) and between patients with mild or moderate OSAS (32.3±20.1°) and the controls (p =0.01). Statistically significant correlations were found between spatial QRS-TA and polysomnographic indices (i.e. AHI, AI, RDT and RDTI). Conlusions Spatial QRS-TA values are significantly higher in patients with OSAS than in controls, thus indicating increased heterogeneity of myocardial action potential. Further long-term prospective studies evaluating the prognostic value of spatial QRS-TA in OSAS patients are needed.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.11.008
       
  • Tangeretin alters neuronal apoptosis and ameliorates the severity of
           seizures in experimental epilepsy-induced rats by modulating apoptotic
           protein expressions, regulating matrix metalloproteinases, and activating
           the PI3K/Akt cell survival pathway
    • Authors: Xiao-qian Guo; Yu-ling Cao; Fang Hao; Zhong-rui Yan; Mei-ling Wang; Xue-wu Liu
      Pages: 246 - 253
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Xiao-qian Guo, Yu-ling Cao, Fang Hao, Zhong-rui Yan, Mei-ling Wang, Xue-wu Liu
      Purpose Epilepsy is complex neural disarray categorized by recurring seizures. Despite recent advances in pharmacotherapies for epilepsy, its treatment remains a challenge due to the contrary effects of the drugs. As a result, the identification of novel anti-epileptic drugs (AEDs) with neuroprotective properties and few side effects is of great value. Thus, the present study assessed the treatment effects of tangeretin using a rat model of pilocarpine-induced epilepsy. Materials and Methods Separate groups of male Wistar rats received oral administrations of tangeretin at 50, 100, or 200mg/kg for 10 days and then, on the 10th day, they received an intraperitoneal injection of pilocarpine (30mg/kg). Subsequently, neuronal degeneration and apoptosis were assessed using Nissl staining and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay procedures. Additionally, the expressions of phosphatidylinositol-3-kinase (PI3K/Akt) pathway proteins, cleaved caspase-3, Bad, Bcl-2, Bcl-xL, and Bax were determined using Western blot analyses. Results Tangeretin reduced the seizure scores and latency to first seizure of the rats and effectively activated the pilocarpine-induced suppression of PI3K/Akt signaling. Additionally, tangeretin effectively regulated the levels of apoptosis-inducing factor (AIF) in mitochondria as well as the expressions of apoptotic pathway proteins. Seizure-induced elevations in the activities and expressions of matrix metalloproteinases (MMPs)-2 and -9 were also modulated. Conclusion The present results indicate that tangeretin exerted potent neuroprotective effects against pilocarpine-induced seizures via the activation of PI3K/Akt signaling and the regulation of MMPs.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.11.011
       
  • Different manifestations of pulmonary embolism in younger compared to
           older patients: Clinical presentation, prediction rules and long-term
           outcomes
    • Authors: Izabela E. Kiluk; Agnieszka Krajewska; Urszula Kosacka; Agnieszka Tycińska; Robert Milewski; Włodzimierz Musiał; Bożena Sobkowicz
      Pages: 254 - 258
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Izabela E. Kiluk, Agnieszka Krajewska, Urszula Kosacka, Agnieszka Tycińska, Robert Milewski, Włodzimierz Musiał, Bożena Sobkowicz
      Purpose Data concerning specific manifestations of pulmonary embolism (PE) among younger patients are scarce. We aimed to evaluate differences in clinical presentation, PE prediction rules, thrombolytic treatment use and PE outcomes in younger (<50 years of age) compared to older patients. Material/methods We studied 238 consecutive patients with proven PE who were retrospectively categorized into three PE probability subgroups according to the revised Geneva score (RGS) and Wells score (WS). Duration of follow-up was up to 115 months. Results Younger patients accounted for 19.7% of the study cohort. Obesity and smoking were significantly more common, while comorbidities were less common (P <0.05) in the younger patients. According to RGS and WS, younger patients were more often categorized into the low PE probability subgroup and rarely into the high probability subgroup (P <0.05). We found no differences in clinical signs, symptoms, and treatment between the two groups. In-hospital (2% vs. 13%) and long-term (12% vs. 36%) mortality rates were significantly lower in younger patients (P =0.003). Conclusions In younger PE patients, despite differences in predisposing factors and PE probability grading as assessed by RGS and WS, clinical features at admission and treatment were similar compared to the older group. Our findings confirmed lower mortality among younger compared to older patients.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.01.001
       
  • Osteogenic differentiation of mesenchymal stem cells is impaired by bone
           morphogenetic protein 7
    • Authors: Paweena Wongwitwichot; Jasadee Kaewsrichan
      Pages: 266 - 272
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Paweena Wongwitwichot, Jasadee Kaewsrichan
      Purpose Mesenchymal stem cells (MSCs) are multipotent adult stem cells and present in practically all tissues but originally identified within the bone marrow (BM). The differentiation potential of these cells is generally impaired when culturing in vitro for cell expansion. The aim of this study is to speedily increase the numbers of bone marrow derived mesenchymal stem cells (BM-MSCs) with substantially maintaining their differentiation potential in vitro and improving bone formation in vivo. Materials and methods BM-MSCs isolated from rats were sequentially cultured in α-MEM containing basic fibroblast growth factor (FGF2) and/or insulin to stimulate proliferation and osteogenic commitment, and in the medium with the addition of bone morphogenetic protein 2 (BMP2) and/or bone morphogenetic protein 7 (BMP7) to arouse differentiation. The expression of genes markedly associating the commitment and differentiation were investigated in vitro using real-time PCR technique and mineralization assay, while the capacity of inducing bone formation by the established conditions was determined in vivo using a rat model. Results The BM-MSCs greatly proliferated with active transcription of runx2 and osterix genes when induced by FGF2 and insulin. The in vitro mineralization was enhanced by BMP2, but the extent was diminished when BMP2 was replaced or supplemented by BMP7. Formation of new small blood vessels was notably detected when the cells were respectively challenged by FGF2 plus insulin and BMP2. Conclusion These data are valuable in choosing growth factors for proper bone repair. However, optimization of the established system would be essential when the cells of human source are applied.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.001
       
  • Treatment of refractory epilepsy patients with autologous mesenchymal stem
           cells reduces seizure frequency: An open label study
    • Authors: Fedor Hlebokazov; Tatiana Dakukina; Svetlana Ihnatsenko; Svetlana Kosmacheva; Michael Potapnev; Antos Shakhbazau; Natalia Goncharova; Michael Makhrov; Pavel Korolevich; Nikolai Misyuk; Victoria Dakukina; Irina Shamruk; Elena Slobina; Sergei Marchuk
      Pages: 273 - 279
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Fedor Hlebokazov, Tatiana Dakukina, Svetlana Ihnatsenko, Svetlana Kosmacheva, Michael Potapnev, Antos Shakhbazau, Natalia Goncharova, Michael Makhrov, Pavel Korolevich, Nikolai Misyuk, Victoria Dakukina, Irina Shamruk, Elena Slobina, Sergei Marchuk
      Purpose Existing anti-epileptic drugs (AED) have limited efficiency in many patients, necessitating the search for alternative approaches such as stem cell therapy. We report the use of autologous patient-derived mesenchymal stem cells (MSC) as a therapeutic agent in symptomatic drug-resistant epilepsy in a Phase I open label clinical trial (registered as NCT02497443). Patients and methods The patients received either standard treatment with AED (control group), or AED supplemented with single intravenous administration of undifferentiated autologous MSC (target dose of 1×106 cells/kg), followed by a single intrathecal injection of neurally induced autologous MSC (target dose of 0.1×106 cells/kg). Results MSC injections were well tolerated and did not cause any severe adverse effects. Seizure frequency was designated as the main outcome and evaluated at 1 year time point. 3 out of 10 patients in MSC therapy group achieved remission (no seizures for one year and more), and 5 additional patients became responders to AEDs, while only 2 out of 12 patients became responders in control group (difference significant, P =0.0135). Conclusions MSC possess unique immunomodulatory properties and are a safe and promising candidate for cell therapy in AED resistant epilepsy patients.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.004
       
  • Methods to evaluate arterial structure and function in children –
           State-of-the art knowledge
    • Authors: Piotr Skrzypczyk; Małgorzata Pańczyk-Tomaszewska
      Pages: 280 - 294
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Piotr Skrzypczyk, Małgorzata Pańczyk-Tomaszewska
      Background With increasing rates of hypertension, obesity, and diabetes in the pediatric population, wide available, and reproducible methods are necessary to evaluate arterial structure and function in children and adolescents. Methods MEDLINE/Pubmed was searched for articles published in years 2012–2017 on methodology of, current knowledge on, and limitations of the most commonly used methods to evaluate central, proximal and coronary arteries, as well as endothelial function in pediatric patients. Results Among 1528 records screened (including 1475 records from years 2012 to 2017) 139 papers were found suitable for the review. Following methods were discussed in this review article: ultrasound measurements of the intima-media thickness, coronary calcium scoring using computed tomography, arterial stiffness measurements (pulse wave velocity and pulse wave analysis, carotid artery distensibility, pulse pressure, and ambulatory arterial stiffness index), ankle-brachial index, and methods to evaluate vascular endothelial function (flow-mediated vasodilation, peripheral arterial tonometry, Doppler laser flowmetry, and cellular and soluble markers of endothelial dysfunction). Conclusions Ultrasonographic measurement of carotid intima-media thickness and measurement of pulse wave velocity (by oscillometry or applanation tonometry) are highly reproducible methods applicable for both research and clinical practice with proved applicability for children aged ≥6 years or with height ≥120cm. Evaluation of ambulatory arterial stiffness index by ambulatory blood pressure monitoring is another promising option in pediatric high-risk patients. Clearly, further studies are necessary to evaluate usefulness of these and other methods for the detection of subclinical arterial damage in children.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.03.001
       
  • Effects of fine air particulates on gene expression in non-small-cell lung
           cancer
    • Authors: Biao Yang; Xinming Li; Dongmei Chen; Chunling Xiao
      Pages: 295 - 301
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Biao Yang, Xinming Li, Dongmei Chen, Chunling Xiao
      Purpose Airborne particulate matter smaller than 2.5μm (PM2.5) has been shown to induce adverse health effects through various mechanisms. However, its effects on gene expression in non-small-cell lung cancer (NSCLC) remain undefined. The aim of this study was to analyze the expression profile of PM2.5-induced adverse health effects on human. Materials and methods We performed RNA sequencing to elucidate key molecular effects of PM2.5 collected from Shenyang China, to identify potential diagnostic markers or therapeutic targets, and further validated these differences in gene expression by using quantitative PCR in A549 and H1299 human non-small-cell lung cancer cell lines. To investigate the functional changes on PM2.5 exposed cells, we carried out the viability assay for the cell counting, and the Boyden chamber assay for invasion. Results We found 143 genes that were expressed at least twice as much, or no more than half as much, in NSCLC cells exposed to PM2.5 than in unexposed cells. Results showed deregulated genes confronted PM2.5 exposure were significantly expressed, but commonly expressed in NSCLC cells. In addition, according to the viability assay and the Boyden chamber assay, PM2.5 exposed cells which have more competent on proliferation and invasion can keep the line with the results in RNA-Seq. Conclusion Our data may provide a more specific understanding of the signaling patterns associated with pathogenesis, and lead to novel markers and therapeutic targets for NSCLC.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2016.12.003
       
  • Mulberry leaf extract decreases digestion and absorption of starch in
           healthy subjects—A randomized, placebo-controlled, crossover study
    • Authors: Jan Józefczuk; Klaudia Malikowska; Aleksandra Glapa; Barbara Stawińska-Witoszyńska; Jan Krzysztof Nowak; Joanna Bajerska; Aleksandra Lisowska; Jarosław Walkowiak
      Pages: 302 - 306
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Jan Józefczuk, Klaudia Malikowska, Aleksandra Glapa, Barbara Stawińska-Witoszyńska, Jan Krzysztof Nowak, Joanna Bajerska, Aleksandra Lisowska, Jarosław Walkowiak
      Purpose Mulberry (Morus alba L.) leaf tea has recently received much attention as a dietary supplement due to the wide range of putative health benefits, such as antidiabetic effects. Nevertheless, data evaluating its influence on carbohydrate metabolism in humans are scarce. The present study aims to investigate the effect of mulberry leaf extract supplementation on starch digestion and absorption in humans. Materials and methods The study comprised of 25 healthy subjects, aged 19–27 years. In all subjects, a starch 13C breath test was performed twice in a crossover and single blind design. Subjects were initially randomized to ingest naturally 13C-abundant cornflakes (50g cornflakes+100ml low fat milk) either with the mulberry leaf extract (36mg of active component-1-deoxynojirimycin) or the placebo and each subject received the opposite preparation one week later. Results The cumulative percentage dose recovery was lower for the mulberry leaf extract test than for the placebo test (median [quartile distribution]: 13.9% [9.9–17.4] vs. 17.2% [13.3–20.6]; p=0.015). A significant decrease was detectable from minute 120 after the ingestion. Conclusions A single dose of mulberry leaf extract taken with a test meal decreases starch digestion and absorption. These findings could possibly be translated into everyday practice for improvement of postprandial glycemic control.

      PubDate: 2017-05-13T07:03:55Z
      DOI: 10.1016/j.advms.2017.03.002
       
  • Ischaemic preconditioning – Current knowledge and potential future
           applications after 30 years of experience
    • Authors: Karolina Stokfisz; Anna Ledakowicz-Polak; Maciej Zagorski; Marzenna Zielinska
      Pages: 307 - 316
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Karolina Stokfisz, Anna Ledakowicz-Polak, Maciej Zagorski, Marzenna Zielinska
      Ischaemic preconditioning (IPC) phenomenon has been known for thirty years. During that time several studies showed that IPC provided by brief ischaemic and reperfusion episodes prior to longer ischaemia can bestow a protective effect to both preconditioned and also remote organs. IPC affecting remote organs is called remote ischaemic preconditioning. Initially, most IPC studies were focused on enhancing myocardial resistance to subsequent ischaemia and reperfusion injury. However, preconditioning was found to be a universal phenomenon and was observed in various organs and tissues including the heart, liver, brain, retina, kidney, skeletal muscles and intestine. Currently, there are a lot of simultaneous studies are underway aiming at finding out whether IPC can be helpful in protecting these organs. The mechanism of local and remote IPC is complex and not well known. Several triggers, intracellular pathways and effectors, humoral, neural and induced by genetic changes may be considered potential pathways in the protective activity of local and remote IPC. Local and remote IPC mechanism may potentially serve as heart protection during cardiac surgery and may limit the infarct size of the myocardium, can be a strategy for preventing the development of acute kidney injury development and liver damage during transplantation, may protect the brain against ischaemic injury. In addition, the method is safe, non-invasive, cheap and easily applicable. The main purpose of this review article is to present new advances which would help to understand the potential mechanism of IPC. It also discusses both its potential applications and utility in clinical settings.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.006
       
  • Impact of exaggerated blood pressure response in normotensive individuals
           on future hypertension and prognosis: Systematic review according to
           PRISMA guideline
    • Authors: Karsten Keller; Kathrin Stelzer; Mir Abolfazl Ostad; Felix Post
      Pages: 317 - 329
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Karsten Keller, Kathrin Stelzer, Mir Abolfazl Ostad, Felix Post
      Purpose Arterial hypertension (aHT) is the leading risk factor for morbidity and mortality worldwide. Blood pressure (BP) deviation at rest is well defined and accompanies risk for cardiovascular events and cardiovascular mortality. A growing body of evidence emphasises that an exaggerated blood pressure response (EBPR) in cardiopulmonary exercise testing (CPET) could help to identify seemingly cardiovascular healthy and normotensive subjects, who have an increased risk of developing aHT and cardiovascular events in the future. Materials and methods The PubMed online database was searched for published studies reporting exercise-related BP and both the risk of aHT and cardiovascular events in the future. Results We identified 18 original studies about EBPR in CPET, which included a total of 35,151 normotensive individuals for prediction of new onset of aHT in the future and 11 original studies with 43,012 enrolled subjects with the endpoint of cardiovascular events in the future. Although an EBPR under CPET is not well defined, a large number of studies emphasise that EBPR in CPET is associated with both new-onset aHT and cardiovascular events in the future. Conclusions A growing number of studies support the hypothesis that EBPR in CPET may be a diagnostic tool to identify subjects with an elevated risk of developing aHT and cardiovascular events in the future.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.010
       
  • Effects of γ-radiation on cell growth, cell cycle and promoter
           methylation of 22 cell cycle genes in the 1321NI astrocytoma cell line
    • Authors: Yaman Alghamian; Ghalia Abou Alchamat; Hossam Murad; Ammar Madania
      Pages: 330 - 337
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Yaman Alghamian, Ghalia Abou Alchamat, Hossam Murad, Ammar Madania
      Purpose DNA damage caused by radiation initiates biological responses affecting cell fate. DNA methylation regulates gene expression and modulates DNA damage pathways. Alterations in the methylation profiles of cell cycle regulating genes may control cell response to radiation. In this study we investigated the effect of ionizing radiation on the methylation levels of 22 cell cycle regulating genes in correlation with gene expression in 1321NI astrocytoma cell line. Methods 1321NI cells were irradiated with 2, 5 or 10Gy doses then analyzed after 24, 48 and 72h for cell viability using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu bromide) assay. Flow cytometry were used to study the effect of 10Gy irradiation on cell cycle. EpiTect Methyl II PCR Array was used to identify differentially methylated genes in irradiated cells. Changes in gene expression was determined by qPCR. Azacytidine treatment was used to determine whether DNA methylation affectes gene expression. Results Our results showed that irradiation decreased cell viability and caused cell cycle arrest at G2/M. Out of 22 genes tested, only CCNF and RAD9A showed some increase in DNA methylation (3.59% and 3.62%, respectively) after 10Gy irradiation, and this increase coincided with downregulation of both genes (by 4 and 2 fold, respectively). Treatment with azacytidine confirmed that expression of CCNF and RAD9A genes was regulated by methylation. Conclusions 1321NI cell line is highly radioresistant and that irradiation of these cells with a 10Gy dose increases DNA methylation of CCNF and RAD9A genes. This dose down-regulates these genes, favoring G2/M arrest.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2017.03.004
       
  • Pro-inflammatory cytokines associated with clinical severity of dry eye
           disease of patients with depression
    • Authors: Małgorzata Mrugacz; Lucyna Ostrowska; Anna Bryl; Agata Szulc; Beata Zelazowska-Rutkowska; Grzegorz Mrugacz
      Pages: 338 - 344
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Małgorzata Mrugacz, Lucyna Ostrowska, Anna Bryl, Agata Szulc, Beata Zelazowska-Rutkowska, Grzegorz Mrugacz
      Purpose The aim of this study was to assess the correlation of inflammatory cytokines levels in tears with severity of dry eye disease in a cohort of patients with depression. Material and methods Tear fluid samples were collected from 32 patients with depression treated with antidepressants, and 34 healthy subjects. Cytokines were assessed by ELISA. All the subjects completed the Beck Depression Inventory and performed the ophthalmic examination, including dry eye tests. Results The tear fluid levels of IL-6, IL-17 and TNF-α in depressive patients were higher than in controls. The clinical severity of dry eye disease correlated significantly with the IL-17 and TNF-α levels. Conclusions Our results suggest a crucial role of inflammatory cytokines, especially IL-17 and TNF-α, in the development of severe dry eye disease in patients with depression. Clarification of the role pro-inflammatory cytokines in the pathogenesis of ocular findings in depressive patients may be useful in establishing immunotherapeutic strategies for this disease.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2017.03.003
       
  • Bacterial infections and hepatic encephalopathy in liver
           cirrhosis–prophylaxis and treatment
    • Authors: Damian Piotrowski; Anna Boroń-Kaczmarska
      Pages: 345 - 356
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Damian Piotrowski, Anna Boroń-Kaczmarska
      Infections are common among patients with liver cirrhosis. They occur more often in cirrhotic patient groups than in the general population and result in higher mortality. One reason for this phenomenon is bacterial translocation from the intestinal lumen that occurs as a consequence of intestinal bacterial overgrowth, increased permeability and decreased motility. The most common infections in cirrhotic patients are spontaneous bacterial peritonitis and urinary tract infections, followed by pneumonia, skin and soft tissue infections. Intestinal bacterial overgrowth is also responsible for hyperammonemia, which leads to hepatic encephalopathy. All of these complications make this group of patients at high risk for mortality. The role of antibiotics in liver cirrhosis is to treat and in some cases to prevent the development of infectious complications. Based on our current knowledge, antibiotic prophylaxis should be administered to patients with gastrointestinal hemorrhage, low ascitic fluid protein concentration combined with liver or renal failure, and spontaneous bacterial peritonitis as a secondary prophylaxis, as well as after hepatic encephalopathy episodes (also as a secondary prophylaxis). In some cases, the use of non-antibiotic prophylaxis can also be considered. Current knowledge of the treatment of infections allows the choice of a preferred antibiotic for empiric therapy depending on the infection location and whether the source of the disease is nosocomial or community-acquired.

      PubDate: 2017-05-18T07:47:13Z
      DOI: 10.1016/j.advms.2016.11.009
       
  • Clinical usefulness of videocapillaroscopy and selected endothelial cell
           activation markers in people with Type 1 diabetes mellitus complicated by
           microangiopathy
    • Authors: Anna Kuryliszyn-Moskal; Wieslaw Zarzycki; Artur Dubicki; Diana Moskal; Bozena Kosztyła-Hojna; Anna Hryniewicz
      Pages: 368 - 373
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Anna Kuryliszyn-Moskal, Wieslaw Zarzycki, Artur Dubicki, Diana Moskal, Bozena Kosztyła-Hojna, Anna Hryniewicz
      Purpose Microvascular abnormalities are one of the most important causes of persistent diabetic complications. The aim of our study was to compare microvascular changes examined by nailfold videocapillaroscopy (NVC) examination with serum concentrations of vascular endothelial growth factor (VEGF), soluble thrombomodulin (sTM) and endothelin-1 (ET-1) in people with Type 1 diabetes with and without microangiopathy. Material/methods The study included 106 people with Type 1 diabetes and 40 healthy controls. All participants were evaluated by extensive clinical, laboratory and capillaroscopic studies. NVC was performed using a stereomicroscope SZ 4045 (Olympus, Germany). The intensity of morphological changes was graded from 0 to 3. Serum levels of VEGF, sTM and ET-1 were determined by an enzyme-linked immunosorbent assay (ELISA). Results Morphological changes were observed by NVC in 86 out of 106 (81%) people with Type 1 diabetes mellitus. Severe capillaroscopic changes (score 3) were seen in 32 out of 54 (59%) people with microangiopathy, but in only seven out of 52 (13%) individuals without microangiopathy. Higher serum concentration of VEGF (p <0.001), ET-1 (p <0.001) and sTM (p <0.05) were demonstrated in people with diabetes complicated with microangiopathy compared to healthy controls. Moreover, comparison between people with and without microangiopathic complications showed a significantly higher capillaroscopic score and sTM serum concentration in the group with retinopathy (p <0.001) nephropathy (p <0.001) and neuropathy (p <0.01). Conclusions Our results suggest that abnormalities in NVC may reflect the extent of microvascular involvement and associated with higher VEGF, sTM and ET-1 serum levels, as well as with microangiopathic complications in diabetic people.

      PubDate: 2017-05-23T08:59:49Z
      DOI: 10.1016/j.advms.2016.11.007
       
  • Prior treatment with non anti-TB antibiotics, and the duration of symptoms
           have no effect on diagnostics of tuberculous meningitis
    • Authors: Marcin Paciorek; Andrzej Pihowicz; Dominik Bursa; Joanna Schöntaler Humięcka; Justyna D. Kowalska; Mateusz Antosiewicz; Magda E. Thompson; Agata Skrzat-Wojdacz; Agnieszka Bednarska; Andrzej Horban
      Pages: 374 - 377
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marcin Paciorek, Andrzej Pihowicz, Dominik Bursa, Joanna Schöntaler Humięcka, Justyna D. Kowalska, Mateusz Antosiewicz, Magda E. Thompson, Agata Skrzat-Wojdacz, Agnieszka Bednarska, Andrzej Horban
      Purpose Our objective was to investigate whether diagnosis of tuberculous meningitis (TBM) with microbiological and molecular analysis was affected by prior empirical non anti tuberculosis antibiotics or by duration of symptoms before lumbar puncture. Materials and methods We retrospectively evaluated medical records of patients with TBM confirmed by positive culture, nucleic acid amplification techniques (NAATs) or Ehrlich–Ziehl–Neelsen staining (EZNs) from the cerebrospinal fluid (CSF) or by characteristic results of biochemical analysis of CSF combined with a typical clinical manifestation. Results 68 adult patients were analyzed. The isolation rates for NAATs, Lowenstein–Jensen (LJ) culture, BACTEC and EZNs were 70.6%, 69.1%, 67.6% and 26.5%, respectively. Biochemical analysis of CSF samples revealed: pleocytosis (median 224 [range 78–380]cells/mm3) with lymphocyte predominance (76 [45–90]%), elevated levels of protein (2.43 [1.50–3.84]g/l) and lactic acid (5.0 [3.9–7.2]mmol/l). Forty (65%) patients received no anti-tuberculosis antibiotic treatment before the diagnostic lumbar puncture. The were no significant differences in the microbiological and biochemical CSF analyses, between the patients who received and those who did not receive non anti-TB empirical antibiotic treatment. The median duration of symptoms before the diagnostic lumbar puncture was 24 (range 11–61) days. No significant differences in microbiological and biochemical analysis of CSF where found when comparing patients with duration of symptoms lasting above and less than the median time. Conclusions Neither prior non anti-TB antibiotic therapy, nor the duration of symptoms before diagnostic lumbar puncture have any effect on confirmation of TBM by microbiological and biochemical CSF analysis.

      PubDate: 2017-05-23T08:59:49Z
      DOI: 10.1016/j.advms.2016.11.003
       
  • Thrombocytopenia associated with TAVI—The summary of possible causes
    • Authors: Maciej Mitrosz; Malgorzata Chlabicz; Katarzyna Hapaniuk; Karol A. Kaminski; Bozena Sobkowicz; Jaroslaw Piszcz; Slawomir Dobrzycki; Wlodzimierz J. Musial; Tomasz Hirnle; Agnieszka M. Tycinska
      Pages: 378 - 382
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Maciej Mitrosz, Malgorzata Chlabicz, Katarzyna Hapaniuk, Karol A. Kaminski, Bozena Sobkowicz, Jaroslaw Piszcz, Slawomir Dobrzycki, Wlodzimierz J. Musial, Tomasz Hirnle, Agnieszka M. Tycinska
      Thrombocytopenia (TP) following transcatheter aortic valve implantation (TAVI) procedure is a common phenomenon but the underlying mechanisms are neither well known nor described. Postinterventional severe TP is related to worse early and late outcome. Moreover, the statement of enhanced platelet and coagulation activation might justify even stronger antiplatelet and anticoagulation therapy following TAVI procedure. Thus, the examination of the pathomechanisms responsible for TP post TAVI seems to be crucial. Several hypotheses have been raised. TP can be caused by insufficient production or impaired platelet renewal. On the other hand, increased platelet activation, consumption and destruction might also be responsible for TP. These findings, mostly related to the procedure alone, need further investigation. Here, we summarize the potential multifactorial causes of post TAVI thrombocytopenia.

      PubDate: 2017-05-28T11:16:38Z
      DOI: 10.1016/j.advms.2017.04.003
       
  • Serum chemerin in children with excess body weight may be associated with
           ongoing metabolic complications – A pilot study
    • Authors: Marta Sledzińska; Agnieszka Szlagatys-Sidorkiewicz; Michał Brzezinski; Katarzyna Kaźmierska; Tomasz Sledziński; Barbara Kamińska
      Pages: 383 - 386
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marta Sledzińska, Agnieszka Szlagatys-Sidorkiewicz, Michał Brzezinski, Katarzyna Kaźmierska, Tomasz Sledziński, Barbara Kamińska
      Purpose The aim of this study was to verify if serum chemerin in children correlates with body weight, fat mass, selected inflammatory markers, parameters of liver function, lipid and glucose metabolism. Materials and methods The study included children aged 5–17 years with normal body weight (<85th BMI percentile, n=43) or overweight (≥85th BMI percentile, n=58). Serum concentrations of chemerin were determined with ELISA. Results Children with excess body weight presented with significantly higher serum concentrations of chemerin. Serum chemerin correlated positively with body weight, absolute BMI and its percentile, fat mass, systolic blood pressure, CRP, ALT, insulin and HOMA-IR. Conclusions Serum level of chemerin may serve as a measure of ongoing obesity-related inflammation, early marker of subclinical liver dysfunction and metabolic syndrome in overweight pediatric patients.

      PubDate: 2017-05-28T11:16:38Z
      DOI: 10.1016/j.advms.2017.04.002
       
  • Effectiveness and safety of ledipasvir/sofosbuvir±ribavirin in the
           treatment of HCV infection: The real-world HARVEST study
    • Authors: Robert Flisiak; Mariusz Łucejko; Włodzimierz Mazur; Ewa Janczewska; Hanna Berak; Krzysztof Tomasiewicz; Iwona Mozer-Lisewska; Dorota Kozielewicz; Andrzej Gietka; Katarzyna Sikorska; Marta Wawrzynowicz-Syczewska; Krzysztof Nowak; Dorota Zarębska-Michaluk; Joanna Musialik; Krzysztof Simon; Aleksander Garlicki; Robert Pleśniak; Barbara Baka-Ćwierz; Iwona Olszok; Krystyna Augustyniak; Wojciech Stolarz; Jolanta Białkowska; Anna Badurek; Anna Piekarska
      Pages: 387 - 392
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Robert Flisiak, Mariusz Łucejko, Włodzimierz Mazur, Ewa Janczewska, Hanna Berak, Krzysztof Tomasiewicz, Iwona Mozer-Lisewska, Dorota Kozielewicz, Andrzej Gietka, Katarzyna Sikorska, Marta Wawrzynowicz-Syczewska, Krzysztof Nowak, Dorota Zarębska-Michaluk, Joanna Musialik, Krzysztof Simon, Aleksander Garlicki, Robert Pleśniak, Barbara Baka-Ćwierz, Iwona Olszok, Krystyna Augustyniak, Wojciech Stolarz, Jolanta Białkowska, Anna Badurek, Anna Piekarska
      Background To evaluate the effectiveness and safety of ledipasvir/sofosbuvir (LDV/SOF)±ribavirin (RBV) regimen in a real-world setting. Methods Patients received a fixed-dose combination tablet containing LDV and SOF with or without RBV, for 8, 12 or 24 weeks. Patients were assessed at baseline, end of treatment, and 12 weeks after the end of treatment. The primary effectiveness endpoint was sustained virologic response 12 weeks after the end of treatment (SVR12). Results Of the 86 patients, aged 20–80 years, 82.6% were HCV genotype 1b-infected and 50.0% were cirrhotic. More than half (52.3%) had previously followed pegylated interferon-containing (PEG-IFN) treatment regimens, and 38.5% were null-responders. SVR12 was achieved by 94.2% of patients. All non-responders were cirrhotic: two demonstrated virologic breakthrough and the remaining three relapsed. All patients treated with an 8-week regimen achieved SVR12 despite having high viral load at baseline (HCV RNA of >1 million IU/mL in 8/10 patients, including one with a viral load of >6 million IU/mL). Adverse events were generally mild and transient. Most frequently, fatigue (22.1%), headache (15.1%), and arthralgia (7.0%) were observed. Laboratory abnormalities included anemia and hyperbilirubinemia. Conclusions Treatment with LDV/SOF±RBV is an effective and safe option for patients with HCV, including those with advanced liver disease or a history of non-response to PEG-IFN-based therapy.

      PubDate: 2017-05-28T11:16:38Z
      DOI: 10.1016/j.advms.2017.04.004
       
  • Clinical features of IgG4-related rhinosinusitis
    • Authors: Machiko Hanaoka; Terumi Kammisawa; Satomi Koizumi; Sawako Kuruma; Kazuro Chiba; Masataka Kikuyama; Satoshi Shirakura; Taro Sugimoto; Tsunekazu Hishima
      Pages: 393 - 397
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Machiko Hanaoka, Terumi Kammisawa, Satomi Koizumi, Sawako Kuruma, Kazuro Chiba, Masataka Kikuyama, Satoshi Shirakura, Taro Sugimoto, Tsunekazu Hishima
      Purpose IgG4-related disease is a systemic disease that affects various organs of the body. Aim of this study is to elucidate the clinical characteristics of IgG4-related rhinosinusitis. Material and methods Clinical features, laboratory findings, radiological and endoscopic findings, associated disease, treatment and prognosis were retrospectively examined in 10 patients with IgG4-related rhinosinusitis. Results The age was 59.1±11.3 years old and male-to-female ratio was 1:1. The chief nasal complaints were hyposmia (n=4), nasal obstruction (n=3), and nothing (n=3). Serum IgG4 levels were elevated in all patients and the value was 740.4±472.4mg/dl. Other IgG4-related diseases were associated in all 10 patients, including IgG4-related sialadenitis (n=6), IgG4-related dacryoadenitis (n=5), and autoimmune pancreatitis (n=5). Imaging findings on CT/MRI were obstruction of the way of elimination (n=10), thickening of the sinus mucous membrane (n=10), and fluid in the sinus (n=6). All of the cases had bilateral findings. Nasal endoscopic findings were chiefly deviated nasal septum (n=5), polyps (n=4), edema of the mucous membrane (n=3). Histologically, abundant infiltration of IgG4 positive plasma cell and lymphocyte and an elevated IgG4+/IgG+ cell ration was detected in all 8 patients and 5 patients, respectively. Endoscopic sinus surgery was performed in 8 patients. Eight patients were treated with steroid therapy for other associated IgG4-related diseases. Symptoms improved in all 6 patients after an initial treatment (endoscopic surgery (n=5) and steroids (n=1)), but one patient suffered relapse. Conclusions IgG4-related rhinosinusitis is a distinct entity of IgG4-related disease, and is associated in patients with multiple IgG4-related diseases.

      PubDate: 2017-06-02T14:03:31Z
      DOI: 10.1016/j.advms.2017.04.001
       
  • Improvements in self-curing composites
    • Authors: Zbigniew Raszewski; Marek Jałbrzykowski
      Pages: 398 - 404
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Zbigniew Raszewski, Marek Jałbrzykowski
      Purpose The purpose of this study was to test the influence of a barbituric acid derivative acting as a catalyst and small amounts of pyrolytic silica in acrylic resins on color stability, solubility and sorption of a composite. Materials and methods A series of two-component powder/liquid resin systems were prepared. Monomer-like mixtures (bis-GMA, TEGDMA, tertiary amine 60/40) and a quartz powder with additions of various silica and barbituric acid derivatives were used. Temperature of the material during polymerization was measured with the use of a thermometer. In addition, the material’s flexural and compressive strength, sorption and solubility were tested pursuant to ISO4049:2009. Results The powder-based acrylic composition in a liquid mixed immediately before use, after an addition of a 0.5% barbituric acid derivative, has a lower temperature during the polymerization process (a reduction from 43°C to 37°C), whereas color stability over time is improved, with ΔE=1.81 for samples of powder mixtures containing between 0.45% of BPO and 0.15% of barbituric acid derivatives. For silanized quartz powder with 0.55% BPO and 0.1% BA+0.5% Aerosil R711, the obtained sorption value was 4.57±0.22μg/mm3, whereas solubility was 1.60±0.32μg/mm3. Conclusions New catalytic system with barbituric acid derivative, improves color stability for samples stored at room condition and under light of high intensity. A two-phase composite (bis GMA TEGDMA/Quartz), with a new catalytic system with barbituric acid derivatives, has a lower self-cured temperature. Adding a small quantity of hydrophobic silica (0.5%) has a significant influence, with reduced sorption and solubility of the material.

      PubDate: 2017-06-16T20:08:23Z
      DOI: 10.1016/j.advms.2017.04.006
       
  • Systematic biobanking, novel imaging techniques, and advanced molecular
           analysis for precise tumor diagnosis and therapy: The Polish MOBIT project
           
    • Authors: Jacek Niklinski; Adam Kretowski; Marcin Moniuszko; Joanna Reszec; Anna Michalska-Falkowska; Magdalena Niemira; Michal Ciborowski; Radoslaw Charkiewicz; Dorota Jurgilewicz; Miroslaw Kozlowski; Rodryg Ramlau; Cezary Piwkowski; Miroslaw Kwasniewski; Monika Kaczmarek; Andrzej Ciereszko; Tomasz Wasniewski; Robert Mroz; Wojciech Naumnik; Ewa Sierko; Magdalena Paczkowska; Joanna Kisluk; Anetta Sulewska; Adam Cybulski; Zenon Mariak; Boguslaw Kedra; Jacek Szamatowicz; Paweł Kurzawa; Lukasz Minarowski; Angelika Edyta Charkiewicz; Barbara Mroczko; Jolanta Malyszko; Christian Manegold; Lothar Pilz; Heike Allgayer; Mohammed L. Abba; Hartmut Juhl; Frauke Koch
      Pages: 405 - 413
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Jacek Niklinski, Adam Kretowski, Marcin Moniuszko, Joanna Reszec, Anna Michalska-Falkowska, Magdalena Niemira, Michal Ciborowski, Radoslaw Charkiewicz, Dorota Jurgilewicz, Miroslaw Kozlowski, Rodryg Ramlau, Cezary Piwkowski, Miroslaw Kwasniewski, Monika Kaczmarek, Andrzej Ciereszko, Tomasz Wasniewski, Robert Mroz, Wojciech Naumnik, Ewa Sierko, Magdalena Paczkowska, Joanna Kisluk, Anetta Sulewska, Adam Cybulski, Zenon Mariak, Boguslaw Kedra, Jacek Szamatowicz, Paweł Kurzawa, Lukasz Minarowski, Angelika Edyta Charkiewicz, Barbara Mroczko, Jolanta Malyszko, Christian Manegold, Lothar Pilz, Heike Allgayer, Mohammed L. Abba, Hartmut Juhl, Frauke Koch
      Personalized and precision medicine is gaining recognition due to the limitations by standard diagnosis and treatment; many areas of medicine, from cancer to psychiatry, are moving towards tailored and individualized treatment for patients based on their clinical characteristics and genetic signatures as well as novel imaging techniques. Advances in whole genome sequencing have led to identification of genes involved in a variety of diseases. Moreover, biomarkers indicating severity of disease or susceptibility to treatment are increasingly being characterized. The continued identification of new genes and biomarkers specific to disease subtypes and individual patients is essential and inevitable for translation into personalized medicine, in estimating both, disease risk and response to therapy. Taking into consideration the mostly unsolved necessity of tailored therapy in oncology the innovative project MOBIT (molecular biomarkers for individualized therapy) was designed. The aims of the project are: (i) establishing integrative management of precise tumor diagnosis and therapy including systematic biobanking, novel imaging techniques, and advanced molecular analysis by collecting comprehensive tumor tissues, liquid biopsies (whole blood, serum, plasma), and urine specimens (supernatant; sediment) as well as (ii) developing personalized lung cancer diagnostics based on tumor heterogeneity and integrated genomics, transcriptomics, metabolomics, and radiomics PET/MRI analysis. It will consist of 5 work packages. In this paper the rationale of the Polish MOBIT project as well as its design is presented. (iii) The project is to draw interest in and to invite national and international, private and public, preclinical and clinical initiatives to establish individualized and precise procedures for integrating novel targeted therapies and advanced imaging techniques.

      PubDate: 2017-06-21T22:19:36Z
      DOI: 10.1016/j.advms.2017.05.002
       
  • The human microbiome
    • Authors: Hubert E. Blum
      Pages: 414 - 420
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Hubert E. Blum
      Until recently, human microbiology was based on the identification of single microbes, such as bacteria, fungi and viruses, frequently isolated from patients with acute or chronic infections. Novel culture-independent molecular biochemical analyses (genomics, transcriptomics, proteomics, metabolomics) allow today to detect and classify the diverse microorganisms in a given ecosystem (microbiota), such as the gastrointestinal tract, the skin, the airway system, the urogenital tract and others, and to assess all genomes in these ecosystems (microbiome) as well as their gene products. These analyses revealed that each individual has its own microbiota that plays a role in health and disease. In addition, they greatly contributed to the recent advances in the understanding of the pathogenesis of a wide range of human diseases. It is to be expected that these new insights will translate into diagnostic, therapeutic and preventive measures in the context of personalized/precision medicine.

      PubDate: 2017-07-21T07:18:26Z
      DOI: 10.1016/j.advms.2017.04.005
       
  • Cells of the innate and adaptive immunity and their interactions in
           inflammatory bowel disease
    • Authors: Zbigniew Kmieć; Marta Cyman; Tomasz Jerzy Ślebioda
      Pages: 1 - 16
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Zbigniew Kmieć, Marta Cyman, Tomasz Jerzy Ślebioda
      Inflammatory bowel disease (IBD) is a group of chronic inflammatory conditions of the gastrointestinal tract that includes two major phenotypes, Crohn's disease and ulcerative colitis that are characterized by different clinical features and different course of the immune response. The exact aetiology of IBD still remains unknown, although it is thought that the diseases result from an excessive immune response directed against microbial or environmentally derived antigens which can be triggered by the disruption of the intestinal epithelial barrier integrity. In this review we present immune mechanisms and interactions between cells of the immune system and tissue environment that contribute to the development and progression of IBD in humans. Since dysregulation of the intestinal immune response is a hallmark of chronic inflammatory conditions, we characterize cells of the innate and adaptive immunity involved in the pathogenesis of IBD and their cross-talks. We describe various subclasses of recently discovered innate lymphoid cells, as well as dendritic cells, macrophages and T cells, including Th17, Th22 and T regulatory cells, present in the intestinal lamina propria and cytokine-mediated regulation of the immune response in IBD, highlighting the role of IL-22 and IL-17A/IL-23 axis. Insights into novel therapeutic modalities targeting certain elements of the immune pathways important for the pathogenesis of IBD have been also shortly presented.

      PubDate: 2017-01-27T21:26:59Z
      DOI: 10.1016/j.advms.2016.09.001
       
  • New insights into the pathogenesis and treatment of irritable bowel
           syndrome
    • Authors: Joanna Oświęcimska; Agnieszka Szymlak; Wojciech Roczniak; Katarzyna Girczys-Połedniok; Jarosław Kwiecień
      Pages: 17 - 30
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Joanna Oświęcimska, Agnieszka Szymlak, Wojciech Roczniak, Katarzyna Girczys-Połedniok, Jarosław Kwiecień
      Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders (FGID), characterized by abdominal pain and a change in stool form that cannot be explained by structural abnormalities. Its prevalence ranges from 9 to 23% of the worldwide population. The pathophysiology of IBS is diverse and not well understood. Biopsychosocial concept assumes that the disease is a product of psychosocial factors and altered at multiple levels of gut physiology interactions. Some aetiological factors have been identified, yet. One of the most important is the disruption of brain-gut mutual communication that leads to visceral hypersensitivity. Also genetic and epigenetic factors are involved. Chronic stress may predispose to IBS as well as exacerbate its symptoms. Both quantitative and qualitative disorders of the gut microbiota are observed. There is also a relationship between the IBS symptoms and the intake of a specific type of food products. In the diarrhoea type of IBS the role of previous gastrointestinal infection is demonstrated. Recent studies have suggested that visceral hypersensitivity in patients with IBS may be secondary to the activation of the immune cells and low-grade inflammation. Clinical symptoms of IBS include abdominal pain and change in bowel habits as well as somatic and psychiatric comorbidities. IBS is diagnosed on the basis of Rome Diagnostic Criteria. Recently, their newest version (Rome IV) has been presented. The aim of this review is to summarize the past decade progress in IBS diagnosis, main pathophysiological aspects and therapeutic management strategy.

      PubDate: 2017-01-27T21:26:59Z
      DOI: 10.1016/j.advms.2016.11.001
       
  • Amyloidosis: A cancer-derived paraproteinemia and kidney involvement
    • Authors: Jolanta Małyszko; Klaudia Kozłowska; Jacek Stanisław Małyszko
      Pages: 31 - 38
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Jolanta Małyszko, Klaudia Kozłowska, Jacek Stanisław Małyszko
      Amyloidosis is the general term describing the extracellular tissue deposition of fibrils composed of low molecular weight subunits of a variety of proteins. There are multiple different human protein precursors of amyloid fibrils. Amyloid deposits are stained using Congo Red and show typical apple-green birefringence in polarized microscopy. Nowadays, a novel technique LMD/MS technique or laser microdissection combined with mass spectrometry help to diagnose amyloidosis. Amyloidosis of the kidney is typically classified as being either one of two types: AL or AA. Less common is the hereditary amyloidosis. Clinical manifestations are usually determined by the type of precursor protein, the tissue distribution, and the amount of amyloid deposition. Renal manifestation is usually present as asymptomatic proteinuria or clinically apparent nephrotic syndrome. In some patients clinical presentation include impaired kidney function with no or mild proteinuria. Patients with renal amyloidosis who progress to end-stage renal disease (ESRD) can be treated with either dialysis or renal transplantation. Diagnosis of amyloidosis is prerequisite to consider treatment options to avoid unnecessary chemotherapy. Treatment of amyloidosis is aimed at decreasing the precursors of fibrillary proteins and/or decrease in synthesis/deposition of amyloid fibrils. It depends upon the type of amyloidosis and cause of excess fibril production.

      PubDate: 2017-02-03T21:55:38Z
      DOI: 10.1016/j.advms.2016.06.004
       
  • Does the OPG/RANKL system contribute to the bone-vascular axis in chronic
           kidney disease' A systematic review
    • Authors: Beata Znorko; Ewa Oksztulska-Kolanek; Małgorzata Michałowska; Tomasz Kamiński; Krystyna Pawlak
      Pages: 52 - 64
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Beata Znorko, Ewa Oksztulska-Kolanek, Małgorzata Michałowska, Tomasz Kamiński, Krystyna Pawlak
      Vascular calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD) and is strongly associated with cardiovascular mortality and morbidity. Accumulating evidence over the past decade has challenged the hypothesis of close interaction between bone and VC what raises the possibility of a common underlying pathophysiological mechanism. Lately, bone regulatory proteins such as: osteoprotegerin (OPG) and Receptor Activator for Nuclear Factor κB Ligand (RANKL) has attracted attention of researchers as a possible key mediators of bone-vascular calcification imbalance. The literature search was carried out using the MEDLINE/PubMed database and a combination of keywords and MeSH terms, and only papers published since January 2005 to July 2016 were selected. The search resulted in 562 potential articles. After selection according to the eligibility criteria, 107 studies fulfilled were included (102 full texts and 5 was case reports). OPG and RANKL plays essential role in the regulation of bone metabolism and may be regarded as a possible link between VC, bone and mineral metabolism in CKD patients. Further studies are required to determine the diagnostic significance of these proteins in evaluation of progression and severity of VC process in CKD patients. Finally, the efficacy and safety, especially in regard to VC, of anti-RANKL therapy in CKD patients requires well-designed prospective, randomized trials.

      PubDate: 2017-07-02T02:07:40Z
      DOI: 10.1016/j.advms.2016.08.001
       
  • Immunohistochemical features of multifocal and multicentric lobular breast
           carcinoma
    • Authors: Ivan R. Ilić; Aleksandar Petrović; Vesna V. Živković; Pavle J. Randjelović; Nikola M. Stojanović; Niko S. Radulović; Dušica Randjelović; Ratko S. Ilić
      Pages: 78 - 82
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Ivan R. Ilić, Aleksandar Petrović, Vesna V. Živković, Pavle J. Randjelović, Nikola M. Stojanović, Niko S. Radulović, Dušica Randjelović, Ratko S. Ilić
      Purpose Patient age, tumor size, axillary lymph node status, expression of estrogen, progesterone and HER2 receptors, as well as the histological grade, are widely accepted prognostic and predictive parameters in breast carcinoma. In invasive lobular carcinoma (ILC), the prognostic factors, such as nodal status and tumor size are believed to be the most valuable ones. The aim of this was set to determine the possible linkage between MFity/MCity of ILC and immunohistochemical predictive (ER, PR, HER2) and prognostic parameters (BRCA1, p53, E-cadherin, Ki-67). Material/methods Out of 2486 cases diagnosed with BC from south-eastern Serbia, the presence ILC was noted in 334 cases. Immunohistochemical characterization of predictive and prognostic parameters in ILC was done. Results The occurrence of multifocal (MFC) and multicentric carcinoma (MCC) was observed in 18.9% of ILC. Bilateral (BL) BCs were found to be statistically significantly more frequent in younger women, compared to those with MFC, MCC or nonMFC/MCC/BL. No significant correlation was found between MFC/MCC and the presence of axillary lymph node metastases, and expression of immunohistochemical predictive and prognostic parameters. Based on literature data and the findings from the current work, MFity/MCtiy might represent negative morphologic prognostic parameters in ILC. Conclusions In 334 cases analyzed, no statistically significant correlations were observed between MF/MC and the expression of immunohistochemical predictive and prognostic parameters.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.003
       
  • Overexpression of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) in
           serum of children after thermal injury
    • Authors: Ewa Matuszczak; Marzena Tylicka; Wojciech Dębek; Anna Sankiewicz; Ewa Gorodkiewicz; Adam Hermanowicz
      Pages: 83 - 86
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Ewa Matuszczak, Marzena Tylicka, Wojciech Dębek, Anna Sankiewicz, Ewa Gorodkiewicz, Adam Hermanowicz
      Purpose The study aims to determinate concentrations of ubiquitin C-terminal hydrolase 1 (UCHL1), which hydrolyzes amino acids from ubiquitin and cleave di-ubiquitins, in serum of children after thermal injury. Patients/methods 42 children scalded by hot water, managed at the Department of Pediatric Surgery, with burns in 4–20% TBSA were included into the study (age 9 months up to 14 years, mean age 2.5±1 years). Blood plasma UCHL1 concentration was assessed in 2–6h, 12–16h, 3d, 5d, and 7d after injury using surface plasmon resonance imaging biosensor. 18 healthy subjects admitted for planned surgeries served as controls. Results The UCHL1 concentration in the blood plasma of patients with thermal injuries reached its peak 12–16h after thermal injury and slowly decreased over time, and still did not reach the normal range on the 7th day after thermal injury. Mean concentrations of UCHL1 after thermal injury were above the range measured in controls (0.12ng/ml): 2–6h after injury – 5.59ng/dl, 12–16h after injury – 9.16ng/dl, 3 days after injury – 6.94ng/dl, 5 days after 5.41ng/dl, 7 days after injury – 4.09ng/dl. Conclusions We observed sudden increase in the concentration of UCHL1 2–16h after thermal injury with the slow decrease in the UCHL1 concentration over the time. UCHL1 concentration was proportional to the severity of the burn. Further studies are needed to determine the mechanisms by which UCHL1 contributes to metabolic response following thermal injury.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.004
       
  • Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor
           type 1 (PAI-1) in diabetic foot syndrome
    • Authors: Arleta Kulwas; Beata Lisewska; Wiesław Jundziłł; Barbara Ruszkowska; Wanda Drewniak; Zofia Ruprecht; Grażyna Gadomska; Danuta Rość
      Pages: 87 - 91
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Arleta Kulwas, Beata Lisewska, Wiesław Jundziłł, Barbara Ruszkowska, Wanda Drewniak, Zofia Ruprecht, Grażyna Gadomska, Danuta Rość
      Purpose The aim was to evaluate tissue plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1) concentration using enzyme linked immunosorbent assay method (ELISA) in diabetic foot syndrome (DFS) as compared to a group of healthy people and patients with diabetes mellitus without symptomatic vascular complications (DM2T). Material/methods Venous blood samples were collected from 90 patients with type 2 diabetes mellitus (30 – DM2T; 60 – DFS). Age-matched controls were also included (n =30). tPA and PAI-1 plasma levels were measured by enzyme-linked immunosorbent assay (ELISA). Results We found a significantly lower concentration of tPA:Ag in patients with DFS in comparison to the DM2T group; tPA concentrations were significantly higher in DM2T as compared to the control group. We observed significantly lower concentration of PAI-1:Ag in DF patients treated for hypertension as compared to patients without hypertension. The tPA:Ag and PAI-1:Ag concentration analysis in DFS depending on age, gender and BMI did not show any significant differences. Conclusions A lower concentration of tPA in patients with DFS may be associated with damage to the endothelial cells, especially in the microvasculature, and the sympathetic nervous system.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.007
       
  • Measuring of quality of life in autoimmune blistering disorders in Poland.
           Validation of disease – specific Autoimmune Bullous Disease Quality of
           Life (ABQOL) and the Treatment Autoimmune Bullous Disease Quality of Life
           (TABQOL) questionnaires
    • Authors: Agnieszka Kalinska-Bienias; Beata Jakubowska; Cezary Kowalewski; Dedee F. Murrell; Katarzyna Wozniak
      Pages: 92 - 96
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Agnieszka Kalinska-Bienias, Beata Jakubowska, Cezary Kowalewski, Dedee F. Murrell, Katarzyna Wozniak
      Purpose Autoimmune bullous dermatoses (AIBD) are rare, severe diseases resulting from some antibodies activity against the different adhesion structures within the skin and/or mucosa. Few studies investigated quality of life (QOL) in AIBD by generic and dermatology-specific instruments, all reporting strong impact on QOL. Recently, disease-specific measurement tools have been developed: Autoimmune Bullous Disease Quality of Life (ABQOL) and Treatment of Autoimmune Bullous Disease Quality of Life (TABQOL) questionnaires. The aim of this study was to test the reliability and validity of ABQOL and TABQOL by developing the first foreign language versions and to evaluate ABQOL and TABQOL in Polish patients. Material and methods The study enrolled 80 patients from the tertiary referral center for AIBD at the outpatient clinic or on admission to the hospital. Sixty six patients completed the 17-item questionnaires of each ABQOL and TABQOL at day 0 and after 5–7 days. Both questionnaires were translated into Polish according to protocol. Results The internal consistency and test–retest reliability were high (Cronbach α =0.95 for ABQOL, α =0.87 for TABQOL), (R =0.98 for ABQOL, R =0.86 for TABQOL). In convergent validity, the correlation of ABQOL and TABQOL was strong (R =0.81), but low with objective disease activity scales. The strongest impact of AIBD on QOL has been observed in flares and in patients with the onset below 70 years of age. The patients with bullous pemphigoid had the highest QOL compared to other AIBD patients. Conclusions The ABQOL and TABQOL are reliable and valid instruments for the assessment of QOL in AIBD.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.002
       
  • Gastroesophageal reflux is not associated with short-term variability of
           parasympathetic activity in children
    • Authors: Jan Krzysztof Nowak; Agata Łaźniak; Aleksandra Lisowska; Zdzisława Kycler; Waldemar Bobkowski; Jarosław Walkowiak
      Pages: 103 - 109
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Jan Krzysztof Nowak, Agata Łaźniak, Aleksandra Lisowska, Zdzisława Kycler, Waldemar Bobkowski, Jarosław Walkowiak
      Purpose A lower parasympathetic activity was described in patients with the gastroesophageal reflux disease. We aimed to determine whether gastroesophageal reflux (GER) episodes are associated with a short-term parasympathetic tone variability in children. Methods In order to address this question we performed simultaneous 24-h esophageal multichannel intraluminal impedance-pH and electrocardiographic monitoring in 16 children (age range 6–18 years), including 8 with asthma and 2 with gastroesophageal reflux disease. After describing duration, height, and acidity of 483 GER episodes we also measured parasympathetic-related heart rate variability parameters in 4 time periods: preceding, containing, following GER, and in-between GERs (control). High frequency (HF) power and root-mean square differences of successive R–R intervals (r-MSSD) were assessed in 2.5-min and 1-min periods, respectively. Results We did not identify the searched short-term parasympathetic tone changes. Conclusions In conclusion, GER episodes and their characteristics were not associated with short-term variability of parasympathetic activity in children.

      PubDate: 2017-02-23T05:25:13Z
      DOI: 10.1016/j.advms.2016.11.012
       
  • Single center experience of subclinical rejections and BK nephropathies by
           kidney allografts’ surveillance biopsies
    • Authors: Beata Naumnik; Jolanta Kowalewska; Tomasz Hryszko; Jerzy Glowiński; Magdalena Durlik; Michal C. Myśliwiec
      Pages: 110 - 115
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Beata Naumnik, Jolanta Kowalewska, Tomasz Hryszko, Jerzy Glowiński, Magdalena Durlik, Michal C. Myśliwiec
      Purpose Acute rejection of the kidney allograft remains the most important factor affecting the long-term graft outcome and is a major predictor of development of chronic damage and graft loss. Several studies have shown that early detection and treatment of subclinical rejection episodes may be beneficial for the graft outcome. The role of protocol (surveillance) biopsies and the value of donor specific antibodies (DSA) monitoring are still debatable. Methods This is a prospective observational study involving seventeen kidney recipients transplanted in north-eastern part of Poland who underwent “zero”, 3-month and 12-month allograft biopsies as well as DSA assessment. Results Histologic analysis of the biopsies showed subclinical acute cellular rejection in 17.6% of patients (two tubulointerstitial, one vascular) at 3-months post transplantation, and additional case of borderline rejection at the 12-month point. Moreover, two cases (11.8%) of polyomavirus BK nephropathy were diagnosed (one at 3 and one at 12 month point). None of the patients developed de novo DSA. Conclusions Our protocol biopsies allowed us to detect significant proportion of patients with subclinical, but histologically relevant acute cellular rejection and BK nephropathy. Early therapeutic intervention had beneficial effects in a 4-year follow up.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.07.005
       
  • Profiling of selected angiogenesis-related genes in serous ovarian cancer
           patients
    • Authors: Paweł Kuć; Radosław Charkiewicz; Dagmara Klasa-Mazurkiewicz; Tomasz Milczek; Barbara Mroczko; Jacek Nikliński; Piotr Laudański
      Pages: 116 - 120
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Paweł Kuć, Radosław Charkiewicz, Dagmara Klasa-Mazurkiewicz, Tomasz Milczek, Barbara Mroczko, Jacek Nikliński, Piotr Laudański
      Purpose Since angiogenesis plays an important role in the pathogenesis of ovarian cancer the aim of the study was to compare the expression of the most relevant angiogenesis-related genes in serous ovarian cancer samples. Genes were divided into 5 subgroups according to their angiogenic potential: growth factors and their receptors; cytokines/chemokines; adhesion molecules and other matrix related proteins; transcriptions factors and signaling molecules; morphogenic factors, and angiogenesis inhibitors. Materials/methods Twenty-nine patients were involved in the study: 20 with serous ovarian cancer and 9 healthy controls. All neoplasms were confirmed by histopathological examination. Healthy ovarian control samples were obtained from women diagnosed with fibroids and had previously scheduled operations. Real-time PCR gene arrays were used to examine the expression of 84 human angiogenesis-related genes and expression of selected proteins was assessed with ELISA. Results Significantly higher expressions of 46 genes were found in the ovarian cancer group compared to the healthy control group. By the use of ELISA we confirmed the expression of three proteins i.e.: angiopoietin-2, angiopoietin-like protein 3, and angiopoietin receptor 2. Only angiopoietin-2 and angiopoietin receptor 2 showed significant differences between ovarian cancer and healthy controls. Conclusions Changes in the expression of selected genes associated with angiogenesis may add new information to the pathogenesis of ovarian cancer. Although the angiopoietin-2 signaling pathway may play an important role in neovascularization in ovarian cancer, the role of angiopoietin-like protein 3 is yet to be established.

      PubDate: 2017-02-23T05:25:13Z
      DOI: 10.1016/j.advms.2016.11.005
       
  • Classical rather than genetic risk factors account for high cardiovascular
           disease prevalence in Lithuania: A cross-sectional population study
    • Authors: Neringa Burokienė; Ingrida Domarkienė; Laima Ambrozaitytė; Ingrida Uktverytė; Raimonda Meškienė; Dovilė Karčiauskaitė; Vytautas Kasiulevičius; Virginijus Šapoka; Vaidutis Kučinskas; Zita Aušrelė Kučinskienė
      Pages: 121 - 128
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Neringa Burokienė, Ingrida Domarkienė, Laima Ambrozaitytė, Ingrida Uktverytė, Raimonda Meškienė, Dovilė Karčiauskaitė, Vytautas Kasiulevičius, Virginijus Šapoka, Vaidutis Kučinskas, Zita Aušrelė Kučinskienė
      Purpose Cardiovascular disease (CVD) mortality accounts for 54% of all deaths in Lithuania, making it the highest among all of the European Union countries. We evaluated the prevalence of several CVD risk factors, including lifestyle, blood biochemistry and genetic predisposition to determine the reasons behind significantly increased CVD prevalence in Lithuania. Materials and methods In total 435 volunteers of Lithuanian ethnicity and stable geographic settlement for 3 generations, had their anthropometric, biochemical and behavioural risk factors measured. A randomly selected sample of 166 volunteers had their 60 CVD risk alleles genotyped. The prevalence of risk alleles and cumulative CVD genetic risk score were compared with population of North-West European origin (CEU) using data from the phase 3 HapMap project. Results CVD was present in 33.8% of study volunteers, 84% of participants consumed alcohol, 21% were current smokers and only 30% of participants engaged in higher levels of physical activity. Also, the average BMI (males 28.3±4.3kg/m2, females 27.3±5.0kg/m2), total cholesterol (males 6.1±1.2mmol/L, females 6.2±1.0mmol/L) and LDL-cholesterol (males 4.1±1.1mmol/L, females 4.1±1.0mmol/L) were above the normal values. The cumulative genetic susceptibility to develop CVD in Lithuanians was only 1.4% higher than in CEU population. Conclusions High BMI and poor population plasma lipid profile are the major contributing factors to high CVD mortality and morbidity in Lithuania. Smoking, alcohol consumption and preliminary genetic predisposition results do not explain the difference in CVD mortality between the Lithuanian and wider European populations. CVD prevention programmes in Lithuania should primarily focus on weight loss and improving blood lipid control.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.08.005
       
  • Orthodontic treatment modifies the oxidant–antioxidant balance in saliva
           of clinically healthy subjects
    • Authors: Piotr Buczko; Małgorzata Knaś; Monika Grycz; Izabela Szarmach; Anna Zalewska
      Pages: 129 - 135
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Piotr Buczko, Małgorzata Knaś, Monika Grycz, Izabela Szarmach, Anna Zalewska
      Purpose The aim of our study was to analyse salivary markers of oxidative stress and an antioxidant response in clinically healthy subjects with fixed orthodontic appliances. Material/methods 37 volunteers were included in the study. Unstimulated (UWS) and stimulated (SWS) whole saliva were analysed for oxidative and antioxidant status and nickel levels immediately before the insertion of the appliances, an one week after and twenty four weeks after the insertion of fixed appliances. Results A significant increase in tiobarbituric acid reactive substance (TBARS) and total oxidant status (TOS) one week, and total protein concentration twenty four weeks after the attachment of orthodontic appliances was found in the saliva. The markers of antioxidant status: superoxide dismutase (SOD), catalase (CAT), uric acid (UA), peroxidase (Px), and total antioxidant status (TAS) were not changed in all periods in UWS. In SWS a significant decrease in SOD1 and CAT was found whereas Px was increased one week after treatment and UA twenty four weeks following treatment. TAS was decreased in UWS and SWS twenty four weeks after orthodontic treatment. Oxidative status index (OSI) was elevated both in UWS and SWS one week after orthodontic treatment in comparison to the results obtained before and twenty four weeks. One week after treatment an increased concentration of nickel was also observed. Conclusions Orthodontic treatment modifies the oxidative–antioxidative balance in the saliva of clinically healthy subjects. Increased nickel concentration in saliva, released from orthodontic appliances, seems to be responsible for changes in the oxidative status of the saliva.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.11.004
       
  • Reference ranges and impact of selected confounders on classic serum and
           urinary renal markers in neonatal period
    • Authors: Monika Miklaszewska; Przemysław Korohoda; Przemko Kwinta; Katarzyna Zachwieja; Tomasz Tomasik; Katarzyna Kiliś-Pstrusińska; Dorota Drożdż
      Pages: 143 - 150
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Monika Miklaszewska, Przemysław Korohoda, Przemko Kwinta, Katarzyna Zachwieja, Tomasz Tomasik, Katarzyna Kiliś-Pstrusińska, Dorota Drożdż
      Purpose Renal injury in term and pre-term neonates may be an antecedent to chronic kidney disease in the child's further life. The aim of the paper was to compile a reference range for selected serum and urinary renal markers in the neonatal period for full-term (FT) and pre-term (PT) newborns. Material and methods The prospective study included 23 FT infants (birth weight – BW≥2500g and gestational age – GA≥37Hbd) and 32 PT children (BW<2500g and GA<37Hbd) in good general condition, without acute kidney injury (AKI) or sepsis. Between the 4th and 28th DOL, urinary concentrations of the studied renal markers (uCr, uNa, uOsm) were determined on a daily basis, while serum creatinine (SCr) was assessed minimum every 48–72h. Results The mean GA and BW of the FT and PT infants were respectively as follows: 38.5±1.7Hbd; 3433±495.2g and 32.7±2.6Hbd; 1836.7±419.8g. For serum glomerular (SCr, eGFR) and tubular markers (FENa, RFI), the median values with normal ranges were compiled. For urinary renal markers (uCr, uNa, uOsm) and those values standardized for kg of body weight, percentile tables for 4–28DOL were elaborated. Conclusions The study has resulted in determining the normal ranges of serum glomerular and tubular renal markers, as well as percentile tables of selected urinary renal parameters during the neonatal period. The percentile tables may prove to be helpful for further standardization of other urinary parameters per urinary creatinine in neonatal population.

      PubDate: 2017-05-08T06:16:54Z
      DOI: 10.1016/j.advms.2016.11.002
       
  • A device for testing the durability and exploitation reliability of dental
           prostheses
    • Authors: Marek
      Abstract: Publication date: September 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 2
      Author(s): Marek Jałbrzykowski
      Purpose The aim of this work was to develop the design of the construction of a stand for testing the operational stability of dental prostheses, designed on the basis of an own idea. There are no devices that would make it possible to test complete structures, including the actual kinematics of motion and the influence of the environment of the oral cavity. Materials and methods Presented were several devices used for testing both fixed and removable prostheses. Attention was paid to their limited possibilities in the area of the tested friction node, kinematics of motion, and the assessment of the influence of the environment of the oral cavity on the tested functional quality. The construction was based on the author's own ideas as well as on a review of the available literature. Results As the final result of the research, simplified images of the stand were presented along with their descriptions. Conclusions The experimental verification of the stand allowed concluding that the research aims have been achieved. First of all, the device allows testing prosthetic structures of different sizes and it is possible to load the tested prostheses in randomly chosen points.

      PubDate: 2017-05-13T07:03:55Z
       
  • CS-PEG decorated PLGA nano-prototype for delivery of bioactive compounds:
           A novel approach for induction of apoptosis in HepG2 cell line
    • Authors: Ahmed A. Abd-Rabou; Hanaa H. Ahmed
      Abstract: Publication date: Available online 12 March 2017
      Source:Advances in Medical Sciences
      Author(s): Ahmed A. Abd-Rabou, Hanaa H. Ahmed
      Purpose Polymer-based nanoparticles are used as vectors for cancer drug delivery. The bioactive compounds (quercetin, ellagic acid and gallic acid) are well known to be not only antioxidants but also chemopreventive candidates against various types of cancers. To circumvent the low bioavailability and the short half-life time obstacles, we hypothesized a novel PLGA nano-platform functionalized with CS and PEG to encapsulate these phytochemicals. This encapsulation will protect the compounds from the phagocytic uptake and deliver PLGA-CS-PEG nano-prototype with high biodegradability and biosafety. Materials and methods Three consequent types of PLGA-based nanocomposites were prepared and characterized. Furthermore, we investigated the newly synthesized nano-formulations against human hepatocellular carcinoma (HepG2) and colorectal cancer (HCT 116) cell lines using cell growth inhibition assays, followed by apoptosis and necrosis assays using flow cytometry to detect the underlying mechanism of HepG2 cell death. Results Through Malvern Zeta Sizer, we recorded that the average diameters of the nano-prototypes ranged from 150 to 300nm. Regarding the cytotoxic activity of quercetin, ellagic acid, and gallic acid-encapsulated PLGA, PLGA-CS, and PLGA-CS-PEG nano-prototypes has been found that they reduce the IC50s of the HepG2 cells values by 2.2, 2.9, 2.8-folds, 1, 1.5, 2.7-folds, and 0.9, 0.7, 1.5-folds, respectively. Mechanistically, the nano-platforms of quercetin seem to be dependent on both apoptosis and necrosis, while those of ellagic acid and gallic acid are mainly dependent on apoptosis. Conclusions CS-PEG-blended PLGA nano-delivery system of quercetin, ellagic acid and gallic acid can potentiate apoptosis-mediated cell death in HepG2 cell line.
      Graphical abstract image

      PubDate: 2017-03-16T17:39:48Z
      DOI: 10.1016/j.advms.2017.01.003
       
 
 
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