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ScienceRise : Biological Science
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ISSN (Print) 2519-8017 - ISSN (Online) 2519-8025
Published by PC Technologe Center Homepage  [6 journals]
  • Possible effects of the exposure to ionizing radiation on the patients
           recovered from COVID-19

    • Authors: Emiliia Domina
      Pages: 4 - 7
      Abstract: The aim. To conduct an analytical literature review on the possible impact of SARS-CoV-2 on the radiosensitivity of the human body and justify the relevance of radiobiological research in this area. Materials and methods. Analysis of data from biological dosimetry / indication of radiation lesions of human peripheral blood T-lymphocyte chromosomes under medical irradiation for comparison with radiosensitivity in the patients recovered from COVID-19 (Scopus International Scientific Metric Database, IAEA guidelines, 2011). Results. With the ongoing COVID-19 pandemic, forecasting and clarifying of the mechanisms of distant effects resulting from interactions between ionizing radiation and the SARS-CoV-2 virus play an important role. The difficulty in solving this problem is caused by the fact that the global science has no exhaustive information on the possible influence of this virus on radiation-induced effects. The attention of the professional community is drawn to the possible impact of SARS-CoV-2 on the radiosensitivity of the body of patients recovered from COVID-19 and a hypothesis is first proposed regarding the mechanism on how to increase it based on the development of systemic long-term inflammation. Therefore, clinical trials of low-dose radiotherapy for the treatment of COVID-19-related pneumonia involve preliminary radiobiological studies to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body' Long-term experience of the author of this paper in biodosimetric (cytogenetic) studies allows her to recommend the peripheral blood lymphocyte test system with chromosome aberration’s analysis as the most radiosensitive cell model. Conclusions. Clinical trials of low-dose radiotherapy for the treatment of COVID-19 pneumonia involve a preliminary radiobiological study to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body' The most optimal approach for the solution of this problem is the use of test-system of human peripheral blood lymphocytes’ culture with the subsequent cytogenetic analysis. It will allow investigating changes in the “dose-effect” “cell cycle stage-effect” dependencies, as well as changes in individual radiosensitivity under the influence of SARS-CoV-2 virus
      PubDate: 2022-05-07
       
  • Peculiarities of the etiological spectrum of household allergens

    • Authors: Svitlana Latsynska, Tetiana Turytska, Olena Snisar, Hanna Chaus
      Pages: 8 - 15
      Abstract: The aim of the study was to study the range of substances that cause respiratory sensitization in adults and children living in Dnipro as of 2019. Materials and research methods. Enzyme-linked immunosorbent assay was used to study the serum of venous blood, which determined the specific IgE of up to 9 allergens that are most common in the home. Kits for quantification of allergen-specific IgE from Vitrotest Specific-IgE, Ukraine were used. Conducted allergy diagnosis for 380 people with certain features of allergy history of various types of allergies, who went to the laboratory to specify the etiological root cause of their disease. Research results. As a result of the analysis of the received data the distribution of allergens on their prevalence among the population of Dnipro was established. The degree of hypersensitivity to each specific allergen and their ability to cross-react with each other was determined. Among the allergens of the household panel, the most dangerous were allergens of molds, which caused a sensitization reaction in 292 people, which was 76.8 % of all surveyed. The top three (in prevalence) of household allergens included: cat epithelium, which caused sensitization in 125 people (32.9 %) and Derm mite. Farinae, sensitivity to which was found in 117 patients (30.7 %). The share of inadequately strong allergopathological reactions is one third of all examined and prevails in people with sensitization to allergic agents of the household panel such as cat epithelium, Derm mite. Farinae and Derm. Pteronyssinus. Hyperractivity to fungi of the genus Candida and mold has been found in 9–12 % of people. The lowest severity of sensitization was observed in the epithelium of the dog and house dust, and low sensitivity in the reactivity structure of allergopathological reactions was observed for allergens of the epidermal group (down and feathers of poultry) and cockroaches. The development of cross-reactions took place between allergens of epidermal origin: there was a hypersensitivity to the hair and epithelium of dogs and cats (r=0.94, P˂0.01), poultry feathers and cockroaches (r=0.99, P˂0.01). This association of cross-reactions is possible, given the theory of minor and major proteins, and is due to their similarity: the similarity of the inclusion in the allergen structures of specific forms of molecules inherent in both allergic agents that may have allergy-stimulating effects. Conclusions. Cross-linking between several types of related allergens is the best way to further investigate this issue. It makes sense to include in the laboratory study a molecular method for the determination of major and minor proteins in the case of the greatest relationship to determine not only a specific allergen as an etiological factor, but also a specific sensitizing protein that is part of them. This is of great importance for subsequent immunotherapy when the removal of only one (major) protein agent can reduce the risk of susceptibility reactions to several types of allergens
      PubDate: 2022-05-07
       
  • Urogenital infections of women of reproductive age caused by conditionally
           pathogenic microorganisms on the background of tobacco smoking

    • Authors: Oksana Starishko, Tetiana Turytska, Anastasia Ovcharenko
      Pages: 16 - 25
      Abstract: The aim: to study changes in the microbiota of the urogenital tract of women of reproductive age in the Dnipro region, which is caused by conditionally pathogenic microorganisms on the background of smoking. Material and methods: biomaterial of patients (scraping from the urogenital tract) who applied to the Center for Laboratory Medicine PE “VIS-MEDIC” in Dnipro region. We analyzed the components of the microbiota of the vagina of women of the surveyed groups (sign of age and smoking) in the period from 2018 to 2021. For the use in an analysis, microbiota data were obtained using the test system Femoflor Screen. Results. Analysis of the results of the study revealed the dependence of the composition of the microbiota of the reproductive tract of women on the use of tobacco products. An increase in indicators for conditionally pathogenic microorganisms was shown. The rate of detection of elevated levels of M. hominis in samples of biological material varied between 6-8 % but was not recorded in all study groups of women. There was also an increase in the frequency of detection of elevated levels of U. urealyticum, U. parvum in samples of biological material, the values of which ranged from 9 to 50 %. Conclusions. The obtained data allowed to assess the effect of tobacco on the composition of the microbiota of the urogenital tract of women and made it possible to use them in measures of social and preventive work, as an indisputable fact to quit smoking. Therefore, there is a need for further research to establish the role of microorganisms involved in restoring the composition of the microbiota after inflammatory processes in women who use and do not use tobacco products. The results may be relevant for the diagnosis of inflammatory diseases, processes caused by opportunistic pathogens of the urogenital tract of women of reproductive age, potentially dangerous occurrence and development of infertility and the basis for social and preventive work among women on the background of smoking
      PubDate: 2022-05-07
       
  • The cytotoxic effect of some synthetic nitrogen-containing heterocyclic
           compounds on cultures of tumour and normal cells and the calculation of
           their ADME, QSAR, and DFT pharmacological properties

    • Authors: Vasyl Vdovin, Sergiy Yarmoluk
      Pages: 26 - 37
      Abstract: The cytotoxic effect of several synthetic nitrogen-containing heterocyclic compounds on cultures of tumour and normal cells and the calculation of their ADME, QSAR, and DFT pharmacological properties The aim. The purpose of our work was to investigate the cytotoxic influence of some synthetic nitrogen-containing heterocyclic compounds, namely imidazole, aurones, and triazole on the culture of tumour cells of melanoma mouse B16, human glioma U251 and normal HEK293 and their ADME, QSAR, and DFT pharmacological properties calculation. Materials and methods. The estimation of cell viability in the conditions of influence of the investigated drugs was carried out by MTT. ADME data screening was performed by the SWISSADME server. QSAR calculations were performed on Way2Drug servers (cancerogenicity was predicted with ROSC-Pred, metabolism – with RA, side effects of drugs were investigated using AdverPred server, LD50 were predicted with Gusar software). The calculation of the functional density (DFT) was carried out using B3LYP and the functional of the exchange-correlation with the base set of 6-31 G (D, P) in the MMFF94 force field in the Avogadro program. The results. It was found that compounds 1 and 2 are toxic for normal cells HEK293, compounds 3, 4, 6 and 7 are low-toxic, and 5 does not inhibit cell growth at all. Our study has demonstrated that in the case of tumour cell line U251 compounds 2, 3 and 7 are non-toxic in general, and substances 1, 4, 5, 6 and 7 have significant toxicity. In a case of cancer cell line B16, compounds 1, 2, 4, 5, and 6 are toxic, and compound 7 is cytotoxic at any concentration. The test compounds (1–7) possess drug-like properties. All compounds meet Lipinski’s “rule of five” criteria. The BOILED-Egg model demonstrates that compound 3 may penetrate blood-brain barrier, all compounds except 1 can be absorbed in the intestine, 2 and 5 can be cleaved in the gastrointestinal tract and 3, 4, 6, and 7 have resistance to digestive enzymes. The analysis of metabolism showed that these compounds can mainly be metabolized by mechanisms of N- and O-glucuronidation and C-oxidation. The obtained data indicate that the smallest toxic effect is achieved with intravenously introduced compounds, and the largest toxicity is achieved with oral administration for compounds 3, 4, 5 and 6. The compounds 1 and 3 are completely noncarcinogenic, the other compounds can affect thyroid glands and hematopoietic system. This result requires further research when introduced into practical application. DFT calculations have shown that all investigated compounds are stable and reactive. Conclusions. Differences in the sensitivity of cell lines and dose-dependent effects of compounds detected during the study should be considered when calculating the optimal working concentrations of drugs. The results of the study are necessary to understand toxic effects on the cell lines B16, HEK293, and U251 and their further use for preclinical studies
      PubDate: 2022-05-07
       
  • Age dynamics of indicators of the microcirculation system in students
           according to laser dopler flowmetry

    • Authors: Оksana Gorna, Daria Horban
      Pages: 38 - 46
      Abstract: The article is devoted to the problem of studying blood microcirculation in healthy individuals at different stages of ontogenesis. The aim of the study was to investigate the peculiarities of the skin blood flow in students aged 17-21. Materials and ways of the research. In order to study the functional state of blood microcirculation the method of laser Doppler flowmetry (LDF) was used. Results. Determination of the age dynamics of the tissue blood flow in subjects aged 17-21 years showed that the parameter of microcirculation in the subjects increased from minimal values at 17 years to maximal values at 21 years. In the female subjects, the value of the microcirculation parameter was higher at 17 years than in the male subjects, while the maximum perfusion value for girls was at 19 years and at 20 years in the male subjects. Assessment of the regulatory devices showed that the amplitude value of low-frequency oscillations in females fell at the age of 19 years and in males at 20 years. The maximal amplitude index of vasomotor oscillations was registered at the age of 19 years both for boys and girls. The amplitude of vasomotor oscillations in the high-frequency range varied in both girls and boys. Three types of LDF-grams were identified among the young adolescents: aperiodic LDF-grams, which correspond to normoemic type of microcirculation, monotonous low amplitude LDF-grams, which correspond to hypooemic type of microcirculation, sinusoidal LDF-grams, which correspond to hyperemic type of microcirculation. Conclusions. As many studies have shown, the heterochronicity of values of blood microcirculation indices is preserved in male and female subjects: in one age section the indices are higher in females, in the other one – in adolescents. This fact reflects the general biological regularity of different maturation of male and female organisms
      PubDate: 2022-05-07
       
  • Identification of species of emeria of turkeys using regression analysis
           of morphometric indicators of ocycists

    • Authors: Petro Liulin, Mykola Bogach, Oleg Getmanets
      Pages: 47 - 52
      Abstract: The aim. Identify the species of oocysts of turkeys by morphometric parameters. Material and methods. Eimeria oocysts obtained from faeces of suspects and patients with spontaneous eimeriosis of turkeys of poultry farms of Kharkiv region served as material for the research. Methods used: parasitological, coprological, light microscopy, morphometry, mathematical and statistical, correlation and regression analysis, ANOVA variation statistics. Results. Morphometrically (n=255) samples of turkey eimeria oocysts were studied and identified to the species: E. gallopavonis (n=50), E. meleagrimitis (n=50), E. adenoids (n=51), E. meleagridis n=53), E. innocua (n=51) according to identification indicators (X1 - length of the oocyst in μm; X2 - width of the oocyst in μm; X3 - area of the oocyst in μm; X4 - eccentricity of the model ellipse; X5 - ratio of the width of the oocyst to its length; X6 - largest curvature and X7 - smallest curvature in its model ellipse poles on the major and minor axes, respectively, in μm, X8 - presence - 1 or absence - 0 polar granules) which are mathematical expressions of morphometric dependences of the structure of eocystic oocysts which are confirmed by the results of regression and correlation analysis. The dependence of the Y oocyte species on seven characteristics has been proved. Conclusions. The morphological features that are mathematical expressions of morphometric dependences of the structure and identification of the species of turkey oocysts are determined with high accuracy. The relative error in determining the type of turkey eimerias does not exceed 2 %
      PubDate: 2022-05-07
       
  • Features of microsporidia Nosema apis and Nosema ceranae (Nosema species)
           development of winter bee (Apis mellifera L.) generation

    • Authors: Hanna Odnosum, Tetiana Yefimenko
      Pages: 53 - 56
      Abstract: Temperature is one of the main abiotic factors affecting the development of causative agents of nosemosis in the bee's body. The aim of the research. To determine the influence of the winter and summer bee nest temperature (20–22 °C and 35–36 °C, respectively) on the duration of Nosema species development of winter bee generation isolated in hoarding cages, and to trace the life cycle of Nosema species of bees with natural infection and overwintering in natural conditions, from November to the beginning of bee brood rearing (February). Materials and methods. For this, 200 bees Apis mellifera sossimai, selected from bee colony in November, were infected by syrup feeding containing Nosema species (5×104 spores per bee). Half of the bees were kept at 35–36 °C and half at 20–22 °C. The stages of Nosema species development were monitored daily for 13 days in midgut smears, stained according to Romanovsky-Giemsa (magnification 900x). Furthermore, with a 15 days frequency, from November to mid-February, 30 bees were selected from 20 bee colonies overwintered in natural conditions, and monitored the stages of Nosema species development at natural infection. Result. It was found that the Nosema species development of winter bee generation artificially infected by Nosema species, was suspended at meronts and sporonts stages until the 13th day from the moment of infection, regardless of the temperature at which the bees were kept in the experiment. In bees selected from bee colonies naturally infected with Nosema species prevailed meronts I, II and in an insignificant amount sporonts, until the end of December, active sporulation took place from the middle of January to the beginning of February. Conclusion. That is, the duration of the life cycle development of Nosema species depends little on temperature but is closely related to the life span of summer and winter bee generation and determined by the biochemistry of their relationships, which allow the parasite to save the host as its habitat
      PubDate: 2022-05-07
       
 
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