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Istanbul Journal of Pharmacy
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ISSN (Print) 2548-0731 - ISSN (Online) 2587-2087
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  • Effects of thymoquinone and etoposide combination on cell viability and
           genotoxicity in human cervical cancer hela cells

    • Authors: Hediye Gamze Nur ÇELEBİOĞLU; Merve BECİT-KIZILKAYA, Aydan ÇAĞLAYAN, Sevtap AYDIN DİLSİZ
      Abstract: Background and Aims: It is thought that thymoquinone might have a crucial role in preventing DNA damage, regulating DNA repair mechanisms, and inhibiting the formation of a cancer. Studies on the cytotoxic and genotoxic effects of thymoquinone together with etoposide in cervical carcinoma cells (HeLa) are not adequate. The objective of this study is to evaluate the ef- fect of combinations with thymoquinone on etoposide cytotoxicity and genotoxicity in HeLa cells.Methods: Cytotoxicity was evaluated by MTT assay and genotoxicity was determined by Comet assay.Results: The IC50 values of thymoquinone were 233.6 μM and 145.5 μM, and the IC50 values of etoposide were 167.3 μM and 52.7 μM for 24 and 48 h, respectively. Thymoquinone significantly decreased the approximate IC50 value of etoposide in doses of 15.63 μM and above for 24 h and 31.5 μM and above for 48 h in a dose-dependent manner. 0.1-5 μM thymoquinone and 1 μM etoposide alone did not cause DNA damage, but at higher doses increased DNA damage significantly in a dose-dependent manner. Thymo- quinone significantly reduced DNA damage induced by 10 μM etoposide at the doses of 0.1-10 μM.Conclusion: Our results show that thymoquinone might increase the cytotoxic and genotoxic effects of etoposide in HeLa cells at high doses and reduce DNA damage at low doses that are not cytotoxic, which suggests that etoposide may increase its anticancer effect at high doses, but comprehensive studies are needed on this subject. This study is a preliminary study and will contribute to the development of new treatment strategies.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Effect of flurbiprofen derivative (SGK597) on cell proliferation and
           apoptosis of breast cancer cell lines

    • Authors: İrem ATLIHAN; Sevgi KOÇYİĞİT SEVİNÇ, Oya ORUN, Özgür YILMAZ, Şükriye Güniz KÜÇÜKGÜZEL, Pinar MEGA TİBER
      Abstract: Background and Aims: The incidence of breast cancer is increasing day by day, especially in women. The search for new drugs against breast cancer is the focus of attention in research. Breast cancer and prostate cancer have remarkable biological similarities. Therefore, the 4-(4-chlorophenyl)-3-(1-(2-fluoro-[1,1'-biphenyl]-4-yl)ethyl)-5-((4-fluorobenzyl)thio)-4H- 1,2,4-triazole (SGK597) compound that is suppressing cell proliferation in prostate cancer, was studied in MCF-7 breast can- cer and MCF-10A mammary epithelial cell lines.Methods: The WST-8 method was used to determine cell viability and cytotoxicity of SGK597 in MCF-7 and MCF10-A cell lines. The JC-1 test was applied to determine changes in mitochondrial membrane potential. The protein expression levels of Bax, Bcl-2, and c-PARP associated with apoptosis were determined using Western blot analysis.Results: After 24 and 48 hours of incubation of SGK597, the IC50 values were 28.74 μM and 17.28 μM for MCF-7; 65.9 μM and 50.5 μM for MCF-10A, respectively. Mitochondrial membrane potential showed a tendency toward depolarization in MCF-7 cells as a result of increasing concentration of SGK597, while the same tendency was not seen for MCF-10A. As a result of western blot experiments, no increase in the Bax/Bcl-2 ratio and c-PARP expression level was observed, indicating no apoptosis.Conclusion: It was observed that the compound SGK597 suppressed MCF-7 cell proliferation. These results indicate that SGK597 may be a candidate compound for use as an anticancer agent.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Investigation of enzyme inhibition potentials, and antioxidative
           properties of the extracts of endemic Stachys bombycina Boiss.

    • Authors: Fatma AYAZ; Nuraniye ERUYGUR
      Abstract: Background and Aims: The genus Stachys L., is represented by around 300 species worldwide. More than 120 taxa, almost 60 of which are endemic, are widely distributed in Turkey, particularly in the eastern and southern regions. Stachys species have traditionally been used for many diseases such as asthma, rheumatism, cough, genital tumors, ulcers, diabetes, hemor- rhoids, kidney stones, and various mental disorders. Among the species, S. bombycina Boiss., namely “arıçayçesi” in Turkish, is one of the near-threatened endemic perennial herbs.Methods: The antioxidant activity of methanol and water extracts of S. bombycina was examined utilizing in vitro techniques, including radical scavenging, such as DPPH, and ABTS, an iron-chelating assay, and the total phenol (TPC) and flavonoid contents (TFC). The extracts were also investigated on enzyme inhibition effects using in vitro spectrophotometric method. HPLC analysis was also used for the determination of the phytochemical profiles of the extracts.Results: Based on our results, the methanol extract of S. bombycina demonstrated higher DPPH and ABTS radical scavenging activity with the IC50 value of 605.7 ± 1.04 and 19.40 ± 0.37 µg/mL, respectively, than the water extract. Otherwise, the water extract was found to have a higher iron chelating activity (IC50= 917.9 ± 3.55 µg/mL) than the methanol extract. The highest TPC of the water extract was determined as 81.07 ± 4.71 µg GAE/mg, although the methanol extract had more TFC at 46.93 ± 1.94 µg QE/mg. In addition, high anti- BChE activity was observed (IC50= 58.09 ± 1.18 µg/mL) in the water extract. In addition, ellagic acid was defined as a major component in the methanol extract, while caffeic acid was detected as the main compound in the water extract.Conclusion: Consequently, the current study is the first to report the antioxidant and enzyme inhibitory properties of S. bom- bycina. According to our findings on S. bombycina, this work can contribute to the development of bioactive agents from natural sources. Moreover, further investigations still need to be conducted on the discovery of the phytoconstituents of S. bombycina responsible for the bioactivity, as well as its potential various biological activities.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Comparative assessment of different nanobodies that inhibit the
           interaction of B7-1/2 with CD28 as a potential therapeutic target for
           immune-related diseases by molecular modeling

    • Authors: Halil İbrahim BULUT; Nail BEŞLİ, Güven YENMİŞ
      Abstract: Background and Aims: Active T cells are central players in the self-defense system as well as in immune-related diseases. Being crucial for T cell activation, the interaction of B7-1/2 with CD28 is associated with T cell activation-related diseases such as alloreactivity in transplantation and autoreactivity in autoimmune disorders. Nanobodies are the recombinant vari- able and single-domain smallest antigen-binding fragments. The focus of this study is to investigate the interactions be- tween B7-1/2 and eight antibodies at the molecular level utilizing computational methods, and to guide the best nanobody for in-vitro and in-vivo studies about immunosuppressiveMethods: After receiving the 3D models of agents via Robetta, molecular docking techniques were used to compare the bind- ing modes and affinities of six nanobodies and two FDA-approved fusion protein models against B7-1/2(CD80/CD86).Results: According to our in silico outputs, we selected the top of model clusters from HADDOCK 2.4 (Z-Score of CD80/CD86:- 2.7 to -1.3/-2.1 to -2.1) and distinguished that 1A1 and 1B2 have higher affinities than Belatacept and Abatacept for the percentage of a calculation scale.Conclusion: Our findings suggest that selected nanobodies show higher affinity by interacting with the CD80/86 epitope regions and provide helpful insights into the design and improvement of further computational investigations of nanobody modeling.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • A smarter, tactical approach for combating Covid-19

    • Authors: Gamze ÇAMLIK; Yıldız ÖZSOY, İsmail Tuncer DEĞİM
      Abstract: As human beings, we communicate with each other just like other creatures. In the same way we need to communicate, COV- ID-19 has to communicate with other viruses. Following the latest Pandemic, combating COVID-19 has become a major need today. Several theories are being formulated and tested for the efficient prevention and treatment of the virus. Vaccination is the ultimate solution but access to the vaccine and getting vaccinated is limited. The purpose of this review paper is to present a new approach. This approach is based on the Quorum sensing of viruses like bacteria. Bacteria use this for communication and it has recently been proven for viruses too. It can be used as a new way or strategy to stop viral communication, therefore restricting the viral spread will possibly help people around the world or reduce the disease’s side effects. This new tactic in- volves the use of functionalized Quantum dots nanoparticles, and when they are coupled with carbon atoms and put to use in different delivery forms, these will be useful for maximum efficacy. The use of carbon quantum dots can be useful to minimize certain possible toxic effects. This may be greatly enhanced by doping boron atoms to the structure to trigger their synergistic effects. We suggest here that the inhaler form of this proposed drug delivery system should simultaneously provide a fairly high efficiency and a less toxic solution.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Evaluation of the role of the pharmacists in the rational use of inhaler
           devices in the treatment of asthma and COPD

    • Authors: Narin ÖZTÜRK; Elif Seyma EKSİ, Zeliha PALA KARA, Sönmez UYDEŞ DOĞAN
      Abstract: Background and Aims: Inhaled drug-delivery is the cornerstone for the treatment of asthma and chronic obstructive pulmo- nary disease (COPD). However, these diseases cannot be adequately controlled in most patients due to the misuse of inhaler devices and poor patient compliance. Patient education is a critical component of treatment and requires the cooperative effort of physicians and pharmacists. This study aimed to evaluate a pharmacists’ ability to use inhaler devices and their practical knowledge, and to assess the extent to which pharmacists have consulted on this issue for the rational drug use of asthma or COPD patients.Methods: A questionnaire containing demographic information, steps for the correct use of inhaler devices, information source for inhaler use, and patient counseling was applied to fifty community pharmacists in Istanbul and the data was evalu- ated.Results: The number of pharmacists who have adequate knowledge about the use of a Metered-Dose Inhaler was 59%. It was 50% for Turbuhaler®, 54% for Diskus®, and 56% for a Capsule Inhaler. Pharmacists operating in community pharmacies for up to 20 years were more knowledgeable about the correct use of inhaler devices, but there was no statistically significant difference according to the pharmacists years in practice (p<0.05 for each device).Conclusion: About half of the pharmacists did not have enough knowledge for the correct use of each inhaler device. This suggests that there is a clear need for special and continuing educational programs for pharmacists to increase their knowl- edge of inhaler use and attitudes towards inhaled therapy to provide better patient counseling and training in the manage- ment of asthma and COPD.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Gene expression profiles for apoptotic and necrotic pathways during
           Amanita phalloides intoxication in mice

    • Authors: Selim KARAHAN; Zehra ATLİ, Ertuğrul KAYA, Feride ÖZDEMİR, Mehmet BOĞA, Sevcan İZGİ
      Abstract: Background and Aims: Amanita phalloides is the deadliest toxic mushroom in the world and causes death from acute liver failure. α-amanitin (α-AMA), the most potent toxin, inhibits RNA polymerase II in hepatocytes, stops protein synthesis, and causes hepatotoxicity. However, the information about the mechanisms underlying hepatotoxicity caused by α-AMA is quite inadequate. This study aims to reveal the complex necrotic and apoptotic mechanisms occurring in mouse hepatocytes de- pending on A. phalloides exposure time in vivo.Methods: BALB-c male mice were divided into 5 groups (n=7): control, α-AMA-2, α-AMA-12, α-AMA-72, and α-AMA-96 groups. A poisoning model was created by oral administration of A. phalloides mushroom extract containing 10 mg/kg of α-AMA to mice and they were sacrificed after 2, 12, 72, and 96 h. Then, TNF-α, Bax, caspase-3, and Bcl-2 gene expression levels in liver tissues were examined by the RT-qPCR method. Time-dependent damage to liver tissues was also evaluated histopathologically.Results: RT-qPCR results showed that proinflammatory cytokine TNF-α mRNA expression levels increased in mouse liver tissues at 2 and 12 h after A. phalloides administration compared among the groups. Bax mRNA expression levels increased in the 12 and 72 h after A. phalloides ingestion. It was observed that caspase-3 mRNA expression levels increased in the 72 and 96 h groups compared among the groups, while Bcl-2 mRNA expression levels decreased in the 72 and 96 h groups.Conclusion: Our findings showed that necrotic mechanisms develop in the early period after A. phalloides mushroom poison- ing, and then apoptotic mechanisms are effective. In conclusion, understanding the mechanisms of A. phalloides-induced hepatotoxicity will provide important information for new treatment strategies to be developed.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Evaluation of summary of product characteristics and patient information
           leaflet of the best-selling drugs in Turkey in terms of readability

    • Authors: Ersan GÜRSOY; Kenan TAŞTAN, Mehmet Akif NAS, İdeal Beraa YILMAZ KARTAL
      Abstract: Background and Aims: Readability can be defined as the easiness or difficulty of texts to be understood by readers. In our study, it was aimed to evaluate the patient information leaflet and the summary of product characteristics in terms of read- ability in Turkish.Methods: Our study is a cross-sectional study. For our study, the best-selling drugs included in the "Turkish Pharmaceutical Market Monitoring Report-8, 2020 Market Status in Terms of Sales Volume and Value" prepared by the Turkish Medicines and Medical Devices Agency in 2021, were evaluated by using Turkish readability formulas (Ateşman and Bezirci-Yılmaz).Results: 138 patient information leaflet and summary of product characteristics of a total of 69 products were evaluated. It has been determined that an average of at least undergraduate education is required for the readability of the texts. The patient information leaflets are significantly shorter than the summary of product characteristics (p=0.000). However, in terms of read- ability, it was easier in Ateşman calculation and more difficult in Bezirci-Yılmaz calculation (p=0.007 and p=0.000, respectively). Conclusion: It has been seen that patient information leaflets are not easy to read texts prepared for patients. While prepar- ing the texts to be read by the patients, the texts should be easily understandable and should be read and understood by people of all education levels.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • The drug burden index medication use in older people in north-east Nigeria

    • Authors: Roland OKORO; Algoni IDRİS
      Abstract: Background and Aims: Evidence has shown that physical and cognitive impairment in older people is linked with the Drug Burden Index (DBI). The study aimed to describe the prescription pattern of DBI medications, estimate the frequency of con- traindicated DBI medication use, determine the rate of exposure to high-risk DBI medications, and identify the potential pre- dictors of exposure to high-risk DBI medications..Methods: This one-year retrospective study was conducted in a secondary healthcare facility. It included patients over 65 years of age who were prescribed at least one anticholinergic and/or sedative medication. The study data were summarized using descriptive statistics, while multivariable logistic regression analysis was used to identify potential predictors of expo- sure to high-risk DBI medications. Statistically, a significant level was set at p < 0.05.Results: Most patients were exposed to cardiovascular drugs (57.5%) followed by antihistamines (25.8%). A total of 23 (6.3%) contraindicated DBI medications were identified. Sixty (19.6%) older patients were prescribed high-risk DBI medications. Pa- tients over 70 years were 3.08 times significantly more likely to be exposed to high-risk DBI medications. Also, patients with a low number of non-DBI co-medications (adjusted odds ratio [AOR] 3.40, 95% CI 1.03 - 11.23), polypharmacy (AOR 7.38, 95% CI 2.20 – 24.73), and those that had contraindicated DBI medications (AOR 3.93, 95% CI 1.14 – 13.53) were significantly more likely to be exposed to high-risk DBI medications.Conclusion: The study demonstrated that most older people in the study were exposed to anticholinergic medications. A considerable proportion of these older people were exposed to contraindicated and high-risk DBI medications. Patients over 70 years of age, a low number of non-DBI co-medications, polypharmacy, and contraindicated DBI prescriptions were the significant predictors of exposure to high-risk DBI medications.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Effects of vitamin D on proliferation, invasion and energy metabolism of
           MCF-7 breast cancer cell line

    • Authors: Emine Nedime KORUCU; Ali UNLU
      Abstract: Background and Aims: 1,25(OH)2D3 (vitamin D) is a pleiotropic hormone with anti-proliferative, pro-apoptotic, and pro- differentiation effects on various cell types, which suggest anti-cancer activity in addition to its classical regulatory action on calcium and phosphate metabolism.Methods: We aimed to put forward the effects of vitamin D in various concentrations and time intervals on cell proliferation and invasion of human estrogen receptor-positive breast cancer (MCF-7) cells by real-time cell electronic sensing system (xCELLigence). A determined dose of the IC50 was applied on samples taken from cell lysates and analyzed the levels of the energy. We also aimed to clarify how vitamin D effects the activity of the protease uPA and their relations with each other.Results: Vitamin D showed a cytotoxic effect on MCF-7 cells in a time and dose dependent manner, with dose of IC50 found to be 140 nM. ATP, ADP, and AMP levels, as well as uPA activities were respectively increased in vitamin D treatment group compared to the control group for the first 24 hours while decreasing at 48, 72, and 96 hours. We determined that 70 and 140 nM vitamin D were decreased in invasion of MCF-7 cells compared to control cells.Conclusion: We observed that proliferation and invasion of breast cancer cells were inhibited by vitamin D treatment on a dose and time dependent manner, and also vitamin D supplementation decreased uPA activity and energy levels. Further studies on the mechanisms of vitamin D and the formulation of none-hypercalcemic analogues in featured are needed.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • A comparative cross-sectional study investigating prevalence and patterns
           of sexual dysfunction among hypertensive and non-hypertensive men

    • Authors: Akinwumi AKİNYEDE; Adedunni OLUSANYA, Hazees MOHAMMED, Olumuyiwa FASİPE, Adesina ARİKAWE
      Abstract: Background and Aims: Erectile dysfunction is prevalent among men with hypertension, but there is paucity of data on preva- lence of other domains of sexual dysfunction (SD) in hypertensives and the general population. The study compared the prevalence and patterns of SD among treated hypertensive male patients with normotensive men. The effect of antihyper- tensive drugs on different domains of sexual function was also investigated in our study.Methods: A total of 195 participants (95 hypertensive and 100 non-hypertensive men) were recruited from the medical out- patient department of a secondary health care facility in Lagos, Nigeria. Sexual function was assessed using International Index of Erectile Function (IIEF) which measures erectile function, orgasmic function, sexual desire, intercourse satisfaction, and overall satisfaction.Results: Sexual dysfunction affecting at least one domain was present in 82.1% of the hypertensive subjects and 52% of non- hypertensive controls (P<0.001). The hypertensive patient had more severe dysfunction in the multiple domains (p< 0.001). The use of methyldopa, furosemide and ß-blockers were associated with significantly lower scores while there was no signifi- cant difference in scores with the use calcium channel blockers, angiotensin converting enzyme inhibitors, angiotensin receptor blockers, thiazide and potassium sparing diuretics. SD was higher in the older age group and with longer duration of hyperten- sion and treatment.Conclusion: SD is common in the adult male population with hypertension significantly increasing the risk. Hypertension is associated with involvement of multiple domains for sexual dysfunction. Use of methyldopa, furosemide and ß-blockers were associated with higher rates of SD in the hypertensive population.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Evaluation of some o-benzenedisulfonimido– sulfonamide derivatives as
           potent antimicrobial agents

    • Authors: Kübra DEMİR-YAZICI; Fatıma Nur YILMAZ, Berna ÖZBEK ÇELİK, Özlen GÜZEL AKDEMİR
      Abstract: Background and Aims: The discovery of new antimicrobials to overcome antimicrobial resistance has always been an important topic for sustainable world health. Since the sulfonamide carrying heterocyclic compounds present a number of advantages as biologically active compounds, in our work reported herein, a small collection of previously synthesized o-benzenedisulfonimido-sulfonamide derivatives were assayed to determine antimicrobial profiles against ten different microorganisms in search of finding promising new antibacterial/antifungal agents.Methods: Eight compounds and their standards were tested against seven bacterial and three fungal strains, including members of Gram-positive, Gram-negative bacteria, and Candida spp., using the microbroth dilution method to measure their MIC (minimum inhibitory concentration) values.Results: All assayed molecules showed different inhibitory effects on ten different targets, with considerable MIC values. Particularly, compound 2 exhibited better antimicrobial activity against the largest number of assayed microorganisms.Conclusion: Further modification and development of o-benzenedisulfonimido-sulfonamide derivatives and additional in vitro studies against putative targets may result in new antimicrobial drug candidates in the near future.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark
           fractions against inflammatory- mediated hemolysis and dyslipidemia in
           rats

    • Authors: Ifeoma Felicia CHUKWUMA; Victor Onukwube APEH, Florence Nkechi NWORAH, Felix Ifeanyi NWAFOR, Lus EZEANYİKA, Victor Nwadiogo OGUGUA
      Abstract: Background and Aims: The inflammatory response, though protective, is the major cause of debilitating diseases when pro- voked excessively or if left unresolved. Brenania brieyi (De Wild) E.M.A.Petit is widely used in folk medicine for the treatment of inflammatory-related diseases. This study investigated the protective effects of methanol and chloroform root bark frac- tions of Brenania brieyi on inflammation-induced hemolysis and dyslipidemia.Methods: Anti-inflammatory activity was investigated by inserting 20 mg of autoclaved cotton pellets into forty-five rats ran- domly distributed into nine groups (n=5), this excluded group 1 (baseline). The extent of hemolysis and dyslipidemia in the inflamed rats was ascertained from hematological parameters, lipid profile, and lipidemic index, while the possible underly- ing mechanisms of inflammation were determined using standard procedures.Results: Treatment with varying doses of the root bark fractions of B. brieyi elicited a significant (p< 0.05) decrease in granu- loma tissue and an increase (p<0.05) in hemoglobin, red and white blood cell count, packed cell volume, and platelets compared with the untreated group 2. A significant (p<0.05) decrease in cholesterol, triacylglycerols, and low-density lipoprotein, and a non-significant (p>0.05) increase in high-density lipoprotein were observed in almost all the test groups compared with group 2. There was a significant restoration of atherogenic and dyslipidemia indices and inhibition of acetic acid-induced vascular per- meability, membrane hemolysis, and platelet aggregation in the fraction-treated groups compared with the control. Conclusion: The findings from this study suggest that B. brieyi inhibits exudation and proliferation of granuloma-forming cells and also has the potential to restore the hematological parameters and lipid anomalies to their physiologic state under chronic inflammation. The possible mechanisms of its action could be inhibition of vascular permeability, stabilization of the membrane, or inhibition of platelet aggregation. This justifies the use of the plant in traditional medicine and also demon- strates its potential as a target for the discovery of new anti-inflammatory agents.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Development and validation of an RP-HPLC method for simultaneous
           determination of curcumin and metronidazole in combined dosage form

    • Authors: Leyla BEBA POJARANİ; E. Vildan BURGAZ, Esra BALOGLU
      Abstract: Background and Aims: The present study aimed to develop and validate a simple reverse phase-high pressure liquid chro- matography (RP-HPLC) method for simultaneous determination of natural compound curcumin and metronidazole in bulk and its combined dosage form.Methods: In situ gel formulation containing curcumin and metronidazole was prepared as a model combined system. The chromatographic separation was accomplished isocratically on Eclipse XDB-C18 (150 mm x 4.6 mm, 5 μm particle size) col- umn using UV-detection at 254 nm. The optimized mobile phase contained a mixture of Phosphate Buffer pH4.5-Acetonitrile (50:50, v/v), and the flow rate was set to 1.0 mL/min with 10 μL injection volume. The method was validated in compliance with International Council for Harmonisation (ICH) standards, and it was successfully used for quality control assays for their combined drug productResults: The results for retention times were 8.60 and 1.40 min for curcumin and metronidazole, respectively. The method indicated linear responses within the concentration ranges of 3.0-80 and 4.8-128 μg/mL with LOD values of 0.62; 1.03 μg/mL and LOQ values of 1.88; 3.13 μg/mL for curcumin and metronidazole, respectively. Precision results were within acceptable limits (RSD<2%), and the determination of the two active substances was not interfered with by any formulation components. Conclusion: The proposed validated RP-HPLC method was successfully applied to determine the total contents of curcumin and metronidazole in situ gel formulation. The validation results showed that the proposed method was simple, specific, and precise, and that it could be used for routine quality control for their combined pharmaceutical application.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
  • Diapin®: A food supplement with diverse therapeutic potentials

    • Authors: Umar Faruk MAGAJİ; Selahattin BOYACI, Umut BULBUL, Sadettin AKÇA, Özlem SAÇAN, Refiye YANARDAĞ
      Abstract: Background and Aims: For many centuries herbs and spices have traditionally been used to treat or manage a variety of dis- eases. The formulation of food supplements containing single or multiple herbs or spices is now popular. These formulations provide biochemical, pharmacological and medicinal benefits due to their diverse phytochemical constituents.Methods: In the present study, Diapin® – a food supplement containing Olea europaea L. leaves extract, Cinnamomum cassia (L.) J. Presl stem extract, Nigella sativa L. seed oil, Cocos nucifera L. oil and vitamin D3 – dissolved in absolute ethanol was evaluated for α-amylase, α-glucosidase, acetylcholinesterase, elastase, neuraminidase, adenosine deaminase and arginase inhibitory activity.Results: The supplement strongly inhibited neuraminidase (IC50 = 0.272 ± 0.007 mg/mL), while adenosine deaminase, ace- tylcholinesterase, elastase and arginase were moderately inhibited (with an IC50 of 4.562 ± 0.052, 5.396 ± 0.563, 5.783 ± 0.058 and 6.800 ± 0.067 mg/mL respectively). The less inhibition activity was on α-amylase and α-glucosidase (IC50 = 9.593 ± 0.582 and 14.010 ± 2.280 mg/mL respectively).Conclusion: The pharmacological activities of Diapin® can be attributed to its opulent phytochemical composition. The pres- ent findings support the folkloric claim of Diapin® supplement having antidiabetic, anticancer, anti-inflammatory, antimicro- bial, anti-ageing, and immune bolstering properties, in addition to the mitigation of Alzheimer’s disease and the alleviation of neurological dysfunction.
      PubDate: Fri, 30 Dec 2022 00:00:00 +030
       
 
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