Publisher: U of the Punjab   (Total: 5 journals)   [Sort by number of followers]

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Intl. J. of Business Reflections     Open Access  
Mycopath     Open Access  
Pakistan J. of Information Management & Libraries     Open Access   (SJR: 0.141, CiteScore: 0)
Pakistan J. of Statistics and Operation Research     Open Access   (Followers: 1, SJR: 0.383, CiteScore: 1)
Punjab University J. of Mathematics     Open Access  
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  This is an Open Access Journal Open Access journal
ISSN (Print) 1729-5521
Published by U of the Punjab Homepage  [5 journals]
  • Screening of selected plant products as alternatives to agar in
           mycological studies

    • Authors: Chinyerum Gloria Ikechi-Nwogu, Eunice Oluchi Nwachukwu
      Abstract:  This study was carried out to examine the use of selected plant products as alternative to agar for mycological studies because agar is expensive and not readily available. Extracts from Aloe vera leaves (aloe), Terminalia ivorensis bark (black afara), Hevea brasiliensis (rubber tree), Abelmoschus esculentus pod (lady finger), seeds of Detarium macrocarpum (sweet detar), Brachystegia eurycoma (naga), Mucuna sloanei (hamburger bean) and Telfairia occidentalis (fluted pumpkin), grains of Triticum sp. (wheat), Zea mays (maize) and Oryza sativa (rice), tubers of Colocasia esculenta (cocoyam), Manihot esculenta (cassava), Dioscorea bulbifera (aerial yam), Ipomoea batatas (sweet potatoes), Dioscorea rotundata  (white yam) and Dioscorea dumetorum (three-leaved yam) were screened as alternative to agar for cost reduction. These plant extracts combined with potato and dextrose in varying concentrations (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 g 100 mL-1), were tested for their gelling potentials. The different concentrations of all the plant products were tested at different periods (1 s to 1 h), under different temperature (20–90 oC). The media did not solidify except potato dextrose cassava starch (PDCS) which solidified at 50 g 100 mL in 30 min and at 29 oC. Seven frequently occurring fungi (Aspergillus niger, Aspergillus flavus, Botrydioplodia theobromae, Fusarium poae, Penicillium chrysogenum, Rhizopus oryzae and Trichoderma harzianum) were cultured on PDCS and PDA (potato dextrose agar) for comparison to evaluate their abilities in supporting fungal growth.  The radial growth and mycelial biomass of the test fungi were recorded and subjected to ANOVA. Mycelial biomass values (g) for each test organism for PDCS and PDA were: 3.77 ± 0.64 and 3.94 ± 0.53 for A. flavus; 4.09 ± 1.51 and 2.14 ± 0.41 for A. niger; 8.08 ± 0.19 and 4.97 ± 1.74 for B. theobromae; 1.04 ± 0.32 and 1.97 ± 0.25 for F. poae; 6.42 ± 0.14 and 5.40 ± 0.16 for P. chrysogenum; 7.10 ± 0.86 and 5.88 ± 0.14 for R. oryzae and 5.08 ± 1.02 and 4.30 ± 2.64 for T. harzianum. The mycelia biomass of A. flavus and T. harzianum on PDCS, are not significantly different at P≤0.05 from those on PDA. While those of A. niger, B. theobromae, F. poae, P. chrysogenum and R. oryzae on PDCS were significantly higher than those on PDA. All the tested fungi exhibited normal growth and sporulation on PDCS when compared with PDA (control), but with better growth on PDCS at P≤0.05. 
      PubDate: 2023-02-27
      Issue No: Vol. 20 (2023)
  • Effect of fungi associated with foliar diseases of Ficus capensis on the
           proximate, anti-nutrient and mineral composition of leaves

    • Authors: Onyekachi F.A. Ibiam, Samuel O. Akpa, Felicia Nmom, Obinna Aru Oje, Smart C. Nwosu, Dennis U. Chidozie, Ezeibe C. Nwaru, Tobechukwu E. Okeke, Franklin C. Ambrose, Olabode O. Adeoye
      Abstract:  The Ficus capensis Thunb leaves are affected by some foliar diseases, which could reduce the nutrient benefits from these leaves. This study aimed at isolation and characterization of fungal pathogens associated with the plant's foliar diseases, analysis of the proximate and phytochemical composition of healthy and diseased fruits and leaves. The effect of isolated fungal species on these proximate and phytochemical contents of leaves was also investigated. Hendersonula sp., Didymeria conferta, Rhizopus stolonifer and Fusarium oxysporium were isolated and characterized as fungi associated with the leaves. Proximate analysis revealed the presence of protein, carbohydrate, lipid, ash, fiber, and moisture. Mineral nutrient analysis revealed the presence of copper, zinc, lead, manganese, magnesium, potassium, sodium, calcium, while that of anti-nutrients revealed saponin, alkaloid, flavonoid, tannin and cyanogenic glycoside, both in the diseased and the healthy leaves and fruits of the plant. The values of the food nutrients and minerals in healthy leaves were significantly different from those of the infected fruits and leaves, with the value of non-nutrients in infected leaves were higher than those in health ones. The non-nutrients in the infected leaves were higher than those in the healthy leaves and fruits of the plants, while the proximate and mineral composition of the supposedly healthy leaves was greater than those of diseased leaves.   
      PubDate: 2023-02-24
      Issue No: Vol. 20 (2023)
  • Mycofiltration potentials of Lentinus squarrosulus (Mont.) Singer on fish
           pond effluent in Onne, Rivers State, Nigeria

    • Authors: Chinyerum Gloria Ikechi-Nwogu, Emmanuel O. Akpaja, Bridget Aruku
      Abstract:  Fish pond effluent discharge into the environment is becoming a nuisance since it contains contaminants that harm the ecology and water quality. In this study, we examined the possibility of using a mushroom Lentinus squarrosulus Mont. in removing impurities from fish pond effluents before discharge into the environment. Untreated effluent was obtained from a fish farm in Eleme Local Government Area of Rivers State, Nigeria and tested for contamination levels using physicochemical and microbiological methods within 24 hours of collection. Sterilized sawdust was bagged, inoculated with mushroom spawn and given two weeks to colonization the substrate. The colonized substrate was prepared for mycofiltration after the third week. With a sterilized stainless-steel spoon, holes were drilled into the substrate's centre, and the untreated water sample was then poured into the holes and allowed to filter through into a sterile container. Filtrate samples were taken to a laboratory for evaluation. Statistical analysis of data obtained after 24–48 h of mycofiltration treatment of aquaculture effluents samples showed a substantial (P≤0.05) decrease in heterotrophic count from 6.7×103 to 0 and total fungal/yeast count from 5.3×103 to 0. The findings revealed that mycofiltration technique is a useful, efficient and affordable technology as it reduced dissolved oxygen from 7.92 to 2.88 mg L-1, biological oxygen demand from 5.97 to 5.62 mg L-1 and chemical oxygen demand from 1.89 to 1.78 mg L-1. Other parameters such as pH, turbidity, electrical conductivity, total dissolved solid, total suspended solid, sulphate, nitrite and phosphate, and algae count were reduced significantly at the end of the filtration process. There were notable variations (P≤0.05) between values obtained from fish pond effluents and that treated with L. squarrosulus, indicating that the mushroom played a promising role in the process of mycofiltration and also showed that mycofiltration is a healthier method for water quality improvement of fish effluent before discharge.

      PubDate: 2023-02-20
      Issue No: Vol. 20 (2023)
  • Effect of Arbuscular Mycorrhizal Fungi (AMF) on development of brown spot
           disease of corn (Zea mays L.) due to Physoderma maydis in Far North

    • Authors: Souleymanou Adamou, Alain Heu, Serge Bertrand Mboussi, Abdou Nourou Kone Nsangou, Paulin Sinama, Eloa Sesseumaga, Zaina Todou Amawissa, Jules Patrice Ngoh Dooh, Philippe Kosma, Zachee Ambang
      Abstract: Corn (Zea mays L.) is affected by a number of diseases that lead to yield loss. One of them is brown spot caused by the soil-borne fungus Physoderma maydis. In this study, the bioprotective potential of arbuscular mycorrhizal fungi (AMF) against this disease was carried out in the nursery and field. In a completely randomized block design, two maize varieties namely CMS9015 (V1) and CMS8704 (V2) were used. In the field, two mycorrhizal treatments (V1AMF, V2AMF) and two controls (CV1, CV2) were developed. In the nursery, the treatments consisted of sterilized and mycorrhized soil with disease inoculation (AMF+DI+SS:V1,V2), sterilized soil without mycorrhization with disease inoculation (DI+SS:V1,V2) and non-sterilized soil (NSS:V1,V2).  Disease incidence and severity, plant height and leaf area, root density, root colonization, nitrogen and phosphorus levels were assessed. In the field, the root colonization by the fungus was 63% as compared to 33% for the mycorrhized plants and the controls. Disease incidence and severity were reduced by 33.7% and 35%, respectively, in the mycorrhized plots in the field. The highest levels of phosphorus (0.74 mg g-1) and nitrogen (9.2 mg g-1) were obtained in the mycorrhized field treatments. In the nursery, disease incidence and severity were 0% in pots containing sterilized soil with or without mycorrhizal fungi. In the nursery, the phosphorus level of 1.07 mg g-1 was recorded in the mycorrhizal treatments and the nitrogen level of 7 mg g-1 was recorded in the leaves of the pots on unsterilized soil. This study provides that AMF can be used for the control of P. maydis in corn.  
      PubDate: 2023-02-18
      Issue No: Vol. 20 (2023)
  • Valorization of a wild saprophytic edible mushroom in western Algeria:
           characterization and nutritional value

    • Authors: Yousra Alim, Soulef Dib-Bellahouel, Zohra Fortas
      Abstract: This study concerned a species of wild edible mushroom, oyster mushroom collected in western Algeria forests, which is well consumed by the local population. These species were identified by macroscopic, microscopic and molecular characteristics. The mushroom’s mycelium was isolated and the optimal conditions for mycelial growth were determined. Variation in different physicochemical parameters (pH, temperature, relative humidity and light) significantly influenced the mycelial growth of the mushroom on potato dextrose agar medium. Optimal mycelia growth was observed at pH 5.6, 25 °C, 100% humidity and low exposure to light. Wheat was used as spawn substrate and straw was used as fructifying substrate. The sporophores of the mushroom were obtained after one month of incubation. We cultivated this wild mushroom from its mycelium and defined the nutritional value of the obtained sporophores. The nutritional analysis was performed according to the most common analytical methods. The composition in moisture, ash, fat, protein, total carbohydrates allowed us to demonstrate that the wild edible mushroom harvested in forests of western Algeria had an important nutritional value. This wild edible mushroom was a remarkable and potential resource for development.
      PubDate: 2023-02-09
      Issue No: Vol. 20 (2023)
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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