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J. of Visualized Experiments     Full-text available via subscription   (Followers: 4, SJR: 0.827, CiteScore: 1)
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Journal of Visualized Experiments
Journal Prestige (SJR): 0.827
Citation Impact (citeScore): 1
Number of Followers: 4  
 
  Full-text available via subscription Subscription journal
ISSN (Online) 1940-087X
Published by MYJoVE Corp Homepage  [1 journal]
  • An Integrated Workflow of Identification and Quantification on FDR
           Control-Based Untargeted Metabolome

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      Abstract: We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra.
      PubDate: Tue, 20 Sep 2022 13:00 EST
       
  • C-arm-Free Simultaneous OLIF51 and Percutaneous Pedicle Screw Fixation in
           a Single Lateral Position

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      Abstract: C-arm-free oblique lumbar interbody fusion at the L5-S1 level (OLIF51) and simultaneous pedicle screw fixation are performed in a lateral position under navigation guidance. This technique does not expose the surgeon or operating staff to radiation hazards.
      PubDate: Fri, 16 Sep 2022 13:00 EST
       
  • Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

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      Abstract: We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.
      PubDate: Fri, 16 Sep 2022 12:00 EST
       
  • An Effective Inoculation Method for Phytophthora capsici on Black Pepper
           Plants

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      Abstract: Pinpricking the basal head of the black pepper plant is a brief and time-saving method to damage it. Here, we provided detailed steps with a video for infecting black pepper plants.
      PubDate: Fri, 16 Sep 2022 12:00 EST
       
  • Transient Transduction of the Strobilated Forms of Echinococcus granulosus

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      Abstract: We describe a rapid transient transduction technique in different developmental stages of Echinococcus granulosus using third-generation lentiviral vectors.
      PubDate: Fri, 16 Sep 2022 12:00 EST
       
  • Optrode Array for Simultaneous Optogenetic Modulation and Electrical
           Neural Recording

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      Abstract: Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.
      PubDate: Thu, 01 Sep 2022 13:00 EST
       
  • Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and
           their Differentiation into the Skeletal Muscle Lineage

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      Abstract: We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.
      PubDate: Wed, 31 Aug 2022 13:00 EST
       
  • Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation
           into Insulin-producing Cells

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      Abstract: Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can be a potential source of MSCs that differentiate into insulin-producing cells (IPCs). In this protocol, we provide detailed steps for the isolation and characterization of rat epididymal Ad-MSCs, followed by a simple, short protocol for the generation of IPCs from the same rat Ad-MSCs.
      PubDate: Mon, 29 Aug 2022 13:00 EST
       
  • Intraoperative Ultrasound in Spinal Surgery

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      Abstract: Here, we present a protocol on the use of intraoperative ultrasound in spinal surgery, particularly in cases of intradural lesions and lesions in the ventral spinal canal when using a posterior approach.
      PubDate: Wed, 17 Aug 2022 12:00 EST
       
  • Protocol for Human Blastoids Modeling Blastocyst Development and
           Implantation

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      Abstract: A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
      PubDate: Wed, 10 Aug 2022 13:00 EST
       
  • Isolation of Preadipocytes from Broiler Chick Embryos

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      Abstract: The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.
      PubDate: Thu, 04 Aug 2022 13:00 EST
       
  • Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

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      Abstract: This protocol describes the induction of Gram-negative monobacterial sepsis in a mouse model system. The model is useful in investigating the inflammatory and lethal host responses during sepsis.
      PubDate: Fri, 29 Jul 2022 14:00 EST
       
  • Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated
           Viral Vectors Encoding Human α-Synuclein

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      Abstract: This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.
      PubDate: Fri, 29 Jul 2022 13:00 EST
       
  • The Immersive Cleveland Clinic Virtual Reality Shopping Platform for the
           Assessment of Instrumental Activities of Daily Living

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      Abstract: Virtual reality (VR) is a powerful yet underutilized approach to advance the diagnosis and treatment of neurological disease. The Cleveland Clinic Virtual Reality Shopping platform combines state-of-the-art VR content with an omnidirectional treadmill to quantify instrumental activities of daily living-a proposed prodromal marker of neurological disease.
      PubDate: Thu, 28 Jul 2022 14:00 EST
       
  • Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in
           Bone Tissue Engineering

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      Abstract: Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration.
      PubDate: Wed, 27 Jul 2022 13:00 EST
       
  • Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering
           Microscopy

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      Abstract: We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.
      PubDate: Mon, 25 Jul 2022 13:00 EST
       
  • A Standardized Approach to Extra-Oral and Intra-Oral Digital Photography

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      Abstract: The present protocol describes a method to capture intra- and extra-oral photographs for digital documentation in the clinical practice of dentistry. The protocol highlights all the critical components involved and the commonly utilized settings to capture dental photographs for clinical documentation.
      PubDate: Fri, 22 Jul 2022 13:00 EST
       
  • A Human Corneal Organ Culture Model of Descemet's Stripping Only with
           Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

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      Abstract: Descemet's Stripping Only is an experimental procedure wherein patients with central corneal guttae resulting from Fuchs Endothelial Corneal Dystrophy have Descemet's membrane stripped for peripheral cells to regenerate the endothelial layer. We present novel methodology simulating DSO in dystrophic human corneas ex vivo with accelerated healing stimulated by eFGF1 (NM141).
      PubDate: Fri, 22 Jul 2022 13:00 EST
       
  • A Modified Surgical Technique for Kidney Transplantation in Mice

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      Abstract: This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.
      PubDate: Fri, 22 Jul 2022 13:00 EST
       
  • Conformable Wearable Electrodes: From Fabrication to Electrophysiological
           Assessment

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      Abstract: Two recent technologies-tattoo and textiles-have demonstrated promising results in cutaneous sensing. Here, we present the fabrication and evaluation methods of tattoo and textile electrodes for cutaneous electrophysiological sensing. These electronic interfaces made of conductive polymers outperform the existing standards in terms of comfort and sensitivity.
      PubDate: Fri, 22 Jul 2022 13:00 EST
       
  • DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in
           Synapse Density

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      Abstract: DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.
      PubDate: Fri, 22 Jul 2022 12:00 EST
       
  • Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total
           Internal Reflection Fluorescence (TIRF) Microscopy

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      Abstract: This protocol is a guide for visualizing dynamic actin and microtubules using an in vitro total internal fluorescence (TIRF) microscopy assay.
      PubDate: Wed, 20 Jul 2022 14:00 EST
       
  • Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic
           Receptors for Functional Studies

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      Abstract: The protocol demonstrates that by performing microtransplantation of synaptic membranes into Xenopus laevis oocytes, it is possible to record consistent and reliable responses of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and γ-aminobutyric acid receptors.
      PubDate: Wed, 20 Jul 2022 14:00 EST
       
  • Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially
           Segmented Functional CoQ Pools

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      Abstract: This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na+, to study the existence of partially segmented functional CoQ pools.
      PubDate: Wed, 20 Jul 2022 13:00 EST
       
  • Data Communication Based on MQTT in a Polymer Extrusion Process

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      Abstract: This work proposes a flexible method for data communication between a film extrusion system and monitoring devices based on a message protocol called Message Queuing Telemetry Transport (MQTT).
      PubDate: Fri, 15 Jul 2022 13:00 EST
       
  • Nanoscale Characterization of Liquid-Solid Interfaces by Coupling
           Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and
           Spectroscopy

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      Abstract: Cryogenic Focused Ion Beam (FIB) and Scanning Electron Microscopy (SEM) techniques can provide key insights into the chemistry and morphology of intact solid-liquid interfaces. Methods for preparing high quality Energy Dispersive X-ray (EDX) spectroscopic maps of such interfaces are detailed, with a focus on energy storage devices.
      PubDate: Thu, 14 Jul 2022 12:00 EST
       
  • Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary
           Adherence Method

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      Abstract: Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method.
      PubDate: Wed, 13 Jul 2022 15:00 EST
       
  • An Integrated Approach for Microprotein Identification and Sequence
           Analysis

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      Abstract: The protocol described here provides detailed instructions on how to analyze genomic regions of interest for microprotein-coding potential using PhyloCSF on the user-friendly UCSC Genome Browser. Additionally, several tools and resources are recommended to further investigate sequence characteristics of identified microproteins to gain insight into their putative functions.
      PubDate: Tue, 12 Jul 2022 13:00 EST
       
  • Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

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      Abstract: This protocol describes the preparation of rat whole sciatic nerve tissue for ex vivo electrophysiological stimulation and recording in an environmentally-regulated, two-compartment, perfused saline bath.
      PubDate: Tue, 12 Jul 2022 13:00 EST
       
  • Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

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      Abstract: Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
      PubDate: Wed, 06 Jul 2022 13:00 EST
       
  • Fertility Sparing Procedure using Carbon Dioxide Fiber Laser Vaporization
           of Ovarian Endometrioma

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      Abstract: In this protocol, CO2 fiber laser technique is demonstrated for the surgical treatment of ovarian endometriosis, which represents a viable alternative in terms of fertility preservation with the major advantage of not being dependent on the surgeon's skills and personal experience.
      PubDate: Wed, 06 Jul 2022 13:00 EST
       
  • High-Throughput Analysis of Non-Photochemical Quenching in Crops Using
           Pulse Amplitude Modulated Chlorophyll Fluorometry

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      Abstract: The protocol introduces a high-throughput method for measuring the relaxation of non-photochemical quenching by pulse amplitude modulated chlorophyll fluorometry. The method is applied to field-grown Glycine max and can be adapted to other species to screen for genetic diversity or breeding populations.
      PubDate: Wed, 06 Jul 2022 12:00 EST
       
  • Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids:
           Pros and Cons for Single Cell Digestion

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      Abstract: This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.
      PubDate: Wed, 06 Jul 2022 12:00 EST
       
  • Economical and Efficient Protocol for Isolating and Culturing Bone
           Marrow-derived Dendritic Cells from Mice

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      Abstract: Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.
      PubDate: Fri, 01 Jul 2022 12:00 EST
       
  • Robotic Left Hepatectomy using Indocyanine Green Fluorescence Imaging for
           an Intrahepatic Complex Biliary Cyst

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      Abstract: Robotic liver surgery has gained more acceptance as a feasible, safe, and effective procedure for the treatment of both benign and malignant indications. However, robotic left hepatectomy is still technically demanding. We describe our surgical technique of a robotic left hepatectomy using indocyanine green fluorescence imaging for a large biliary cyst.
      PubDate: Fri, 24 Jun 2022 13:00 EST
       
  • Robotic Pancreatoduodenectomy for Pancreatic Head Cancer: a Case Report of
           a Standardized Technique

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      Abstract: Robotic pancreatoduodenctomy (RPD) has been highly standardized in recent years and may be used in selected patients with pancreatic head cancer, including those with a replaced right hepatic artery. This case report describes a standardized and reproducible technique for RPD, which includes the approach of the Dutch LAELAPS-3 training program to an aberrant vasculature.
      PubDate: Fri, 24 Jun 2022 12:00 EST
       
  • Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of
           Alpha-Synuclein Structural Dynamics Under Physiological Conditions

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      Abstract: The structural ensemble of monomeric alpha-synuclein affects its physiological function and physicochemical properties. The present protocol describes how to perform millisecond hydrogen/deuterium-exchange mass spectrometry and subsequent data analyses to determine conformational information on the monomer of this intrinsically disordered protein under physiological conditions.
      PubDate: Thu, 23 Jun 2022 14:00 EST
       
  • Self-Assembly of Microtubule Tactoids

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      Abstract: This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.
      PubDate: Thu, 23 Jun 2022 13:00 EST
       
  • Microfluidic Tools for Probing Fungal-Microbial Interactions at the
           Cellular Level

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      Abstract: Owing to the opacity of soil, interactions between its constituent microbes cannot easily be visualised with cellular resolution. Here, two microfluidic tools are presented, which offer new opportunities for investigating fungal-microbial interactions. The devices are versatile and simple to use, enabling high spatiotemporal control and high-resolution imaging at the cellular level.
      PubDate: Thu, 23 Jun 2022 13:00 EST
       
  • Determining Binding Affinity (KD) of Radiolabeled Antibodies to
           Immobilized Antigens

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      Abstract: Here, a method is described to determine the binding affinity (KD) of radiolabeled antibodies to immobilized antigens. KD is the equilibrium dissociation constant that can be determined from a saturation binding experiment by measuring the total, specific, and nonspecific binding of a radiolabeled antibody at various concentrations to its antigen.
      PubDate: Thu, 23 Jun 2022 13:00 EST
       
  • Non-Destructive Evaluation of Regional Cell Density Within Tumor
           Aggregates Following Drug Treatment

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      Abstract: The present protocol develops an image-based technique for rapid, non-destructive, and label-free regional cell density and viability measurement within 3D tumor aggregates. Findings revealed a cell-density gradient, with higher cell densities in core regions than outer layers in developing aggregates and predominantly peripheral cell death in HER2+ aggregates treated with Trastuzumab.
      PubDate: Tue, 21 Jun 2022 14:00 EST
       
  • Pooled shRNA Library Screening to Identify Factors that Modulate a Drug
           Resistance Phenotype

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      Abstract: High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).
      PubDate: Fri, 17 Jun 2022 15:00 EST
       
  • Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

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      Abstract: Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.
      PubDate: Thu, 16 Jun 2022 14:00 EST
       
  • Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated
           CRISPR/Cas9 Gene Editing

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      Abstract: The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.
      PubDate: Thu, 16 Jun 2022 14:00 EST
       
  • Standardized Rat Coronary Ring Preparation and Real-Time Recording of
           Dynamic Tension Changes Along Vessel Diameter

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      Abstract: The present protocol describes the wire myograph technique for measuring vascular reactivity of the rat coronary artery.
      PubDate: Thu, 16 Jun 2022 14:00 EST
       
  • Hydrogel Arrays Enable Increased Throughput for Screening Effects of
           Matrix Components and Therapeutics in 3D Tumor Models

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      Abstract: The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.
      PubDate: Thu, 16 Jun 2022 13:00 EST
       
  • A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic
           Resorption In Vitro

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      Abstract: Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.
      PubDate: Thu, 16 Jun 2022 13:00 EST
       
  • Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

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      Abstract: Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.
      PubDate: Wed, 15 Jun 2022 13:00 EST
       
  • Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from
           Human Pluripotent Stem Cells

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      Abstract: Recent advances in human induced pluripotent stem cell differentiation protocols allow for the stepwise derivation of organ-specific cell types. Here, we provide detailed steps for the maintenance and expansion of iPSC-derived airway basal cells and their differentiation into a mucociliary epithelium in air-liquid interface cultures.
      PubDate: Tue, 14 Jun 2022 14:00 EST
       
  • Preparation of Enantiopure Non-Activated Aziridines and Synthesis of
           Biemamide B, D, and epiallo-Isomuscarine

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      Abstract: In this study, we prepare both enantiomers of aziridine-2-carboxylate, which are used in the asymmetric synthesis of alkaloids, including biemamide B and D, and (-)-epiallo-isomuscarine.
      PubDate: Mon, 13 Jun 2022 15:00 EST
       
 
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