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Functional Foods in Health and Disease
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Online) 2160-3855
Published by The Food Science Publisher Homepage  [2 journals]
  • Nanoencapsulation and identification of phenolic compounds by
           UPLC-Q/TOF-MS2 of an antioxidant extract from Opuntia atropes

    • Authors: Héctor E. Martínez-Flores; Eunice Tranquilino-Rodríguez, José O. Rodiles-López, Guillermo C. G. Martínez-Avila
      Abstract: Background: Nanoencapsulation is a technique that protects bioactive compounds such as polyphenolic compounds from environmental factors, through a biopolymer that acts as a wall system. Cladodes of O. atropes are an important source of polyphenolic compounds, flavonoids being the most abundant, these are mainly in the form of glycosides and their consumption has been related to a decrease in glucose and lipid profile through the elimination of intracellular radicals.Purpose of the study: To study the formulation and process parameters that allow obtaining the best experimental conditions to nanoencapsulation and protect the polyphenolic compounds of the O. atropes extract.Methods: Wall materials applied for nanoencapsulation (soy protein isolate, calcium caseinate , and maltodextrin). A Box Behnken 33 design was used; wall material extract of O. atropes (1/1, 2.5 / 1, 4/1), temperature (95, 105 and 115 ° C) and spray (40, 70 and 100%), to evaluate the yield, feeding speed, particle size, encapsulation efficiency, phenolic acids, flavonoids, DPPH• and ABTS•+. Polyphenolic compounds were identified by UPLC-Q / TOF-MS2.Results: Maltodextrin presented better properties in the encapsulation of the extract of O. atropes. The Box Behnken 33 design made it possible to identify the treatment with the appropriate quality characteristics for the nanoencapsulation process; Formulation 2.5 / 1 (maltodextrin / O. atropes extract), 115 ° C and 100% spray, to obtain 85.22% yield, feeding speed of 9 mL / h, the particle size of 154.77 nm, 55.69 % encapsulation efficiency, phenolic acids of 0.95 mg GAE / g, flavonoids of 7.02 mg QE / g, and antioxidant activity in DPPH• of 0.29 mg GAE / g and  ABTS•+ of 0.45 mg GAE / g. The polyphenolic profile was characterized, and it was confirmed that nanoencapsulation protects some isomers of isorhamnetin 3-O-rhamnosido-7-O- (rhamnosyl-hexoside) and p-coumaric acid 4-O-glucoside.Conclusion: Maltodextrin is an excellent encapsulating material of the O. atropes extract, the formulation and process conditions that favored all the response variables were identified and it was shown that nanoencapsulation protects the polyphenolic compounds.Keywords: O. atropes extract, nanoencapsulation, polyphenolic profile, UPLC-Q / TOF-MS2.
      PubDate: Thu, 31 Dec 2020 17:49:15 +000
  • Brazilian green propolis promotes the cytoprotective expression of heme
           oxygenase-1 against oxidative stress injury in murine myoblast cells

    • Authors: Naoki Chinen; Wataru Otsu, Kazuki Ohuchi, Shiori Ando, Shinsuke Nakamura, Hiroyuki Kono, Masamitsu Shimazawa, Hideaki Hara
      Abstract: Background: Sarcopenia is a progressive skeletal muscle disorder characterized by the progressive loss of muscle mass and function, resulting in physical disability and mortality. Although sarcopenia impacts a large proportion of elderly individuals, no effective treatment for this disease has yet been identified. The excessive production of reactive oxygen species (ROS) can damage tissues and promote aging, and the daily use of dietary antioxidants can be effective for maintaining skeletal muscle health. Propolis, a natural substance that is collected by honey bees, has been used as traditional medicine, and many reports have described its antioxidative properties. However, how propolis exhibits cytoprotective effects and antioxidative effects in skeletal muscles remains unclear. The purpose of this study was to investigate the antioxidative effects of ethanol-extracted Brazilian green propolis (EEBP, from Baccharis dracunculifolia) and its three constituents using an in vitro myoblast cell model.Methods: Murine myoblast C2C12 cells were treated with either EEBP or its constituents, including caffeic acid, trans-ferulic acid, and p-coumaric acid, in the presence of 100 or 300 mM H2O2 to induce oxidative stress injury. The cell death ratio and cell viability were assessed by Hoechst 33342 and propidium iodide staining and the WST-8 assay, respectively. Simultaneously, intracellular ROS production was measured by CM-H2DCFDA [5-(and-6)-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester] assay. Finally, immunoblotting was performed in myoblast cell lysates to assess the expression level of an antioxidative enzyme, heme oxygenase-1 (HO-1).Results: We demonstrated that EEBP significantly reduced H2O2-induced cell death at a concentration of 3 µg/ml in myoblasts. Additionally, caffeic acid at 100 µM improved cell viability under oxidative stress conditions, but not trans-ferulic acid or p-coumaric acid. Both EEBP and caffeic acid inhibited the H2O2-induced increase in ROS production. Finally, HO-1 expression was increased by treatment with either EEBP or caffeic acid. The increase in HO-1 expression induced by H2O2 was enhanced in the presence of EEBP and caffeic acid.Conclusions: These findings indicated that EEBP has protective effects against oxidative damage in C2C12 murine myoblast cell line. Caffeic acid is an EEBP constituent that contributes to cytoprotective activity. EEBP may act as an inducer of HO-1 to prevent oxidative stress-induced myoblast death.Keywords: C2C12 murine myoblast cells, heme oxygenase-1, oxidative stress, propolis, reactive oxygen species
      PubDate: Wed, 16 Dec 2020 19:42:56 +000
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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