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Journal of Parasite Science
Number of Followers: 2  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2599-0993 - ISSN (Online) 2656-5331
Published by Universitas Airlangga Homepage  [51 journals]
  • Identifikasi Parasit Pentastomida pada Biawak Air (Varanus salvator) yang
           akan dikonsumsi

    • Authors: Andhika Yudhantama Subroto; I Komang Wiarsa Sardjana, Moh Sukmanadi, E Djoko Poetranto, Kusnoto Kusnoto, Agus Sunarso
      Abstract: This study aims to determine the pentastomide parasite that infects the digestive tract of giant water lizards examined in Laboratory of Parasitology Department, Faculty of Veterinary Medicine, Airlangga University. In this study, three sample were found to be infected with pentastomide parasite in the lung. The phase found was adult phase pentastomide; it has pentastomide spermatheca which shows that this parasite is female. 
      PubDate: Sat, 27 Jun 2020 07:18:20 +000
      DOI: 10.20473/jops.v4i1.20276
  • Identifikasi dan Pola Infestasi Caplak pada Biawak Air (Varanus salvator,
           Byers, d. 2000)

    • Authors: Kartika Aditiya Amelia; Rahaju Ernawati, Poedji Hastutiek, muchammad Yunus, Boedi Setiawan, Agus Sunarso
      Abstract: The aim of this study is to identify the type of ticks infestation pattern on water monitor lizard (Varanus salvator, Byers, D. 2000) with regard parameters water monitor lizard species, stadium and region of the water monitor lizard’s body. The total samples of 250 ticks from 30 water monitor lizard (18 male lizards and 12 female lizards) were collected every day starting from noon to evening during July to August 2019 at the monitor slaughtering place in Buduran District, Sidoarjo Regency. The tick samples were identified by the permanent mounting slide method without staining and put in bottles containing 10% KOH for 1-10 hours, then each stadium used a nikon eclipse E100 microscope with a 40x magnification binocular microscope. Data was analyzed using Multiple Correspondance Analytics. The result indicate that 30 V. salvator (100%) are positive infested by tick and the samples obtained consisted of 133 Aponomma sp. and 117 Amblyomma sp. in all regions of the body.
      PubDate: Sat, 27 Jun 2020 07:18:14 +000
      DOI: 10.20473/jops.v4i1.20274
  • Identifikasi Protozoa pada Darah dan Saluran Pencernaan Biawak Air
           (Varanus salvator)

    • Authors: Azizah Bilqis Nurkarimah; Mufasirin Mufasirin, Ratna Damayanti, Lucia Tri Suwanti, Boedi Setiawan, Endang Suprihati
      Abstract: The aim of this research is to identify the variaous of protozoa in the bloodanddigestive tract on water monitor lizard (Varanus salvator) was captured in Sidoarjo, East Java. This research was taken on March until June 2019. This research used a non-experimental method through an observation study. As many as 50 water monitor lizard were used this research and examined at the Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga.The samples of this research were made in thin blood smear using Giemsa stain, while fecal examination using native method, sucrose flotation, and modified Ziehl-Neelsen stain.The results showed that two various of protozoa found were single infection. Observations on the blood was obtained Haemogregarina sp. (14%) and observations of feces obtained Eimeria sp. (2%). The conclusion of this research indicate that type of protozoa was Haemogregarina sp. and Eimeria sp. were found on water monitor lizard(Varanus salvator).
      PubDate: Sat, 27 Jun 2020 07:18:09 +000
      DOI: 10.20473/jops.v4i1.20273
  • Identifikasi Cacing Nematoda pada Sekum dan Kolon Sapi Kurban yang
           Dipotong saat Idul Adha 1439 H di Wilayah Surabaya Timur

    • Authors: Jihaan Haajidah; Moh Sukmanadi, Kusnoto Kusnoto, Endang Suprihati, Lianny Nangoi, Poedji Hastutiek
      Abstract: The aim of this research was to detect the presence of nematode worms that infects sacrificial cattle slaughtered duringEid al-Adha 1439 H in East Surabaya. This research used 24 sampels of sacrificial cattle and used digestive tract surgical method. Based on the result of the examination using a microscope with 100x magnification, there are positive samples infected with nematode worms. In caecum cattle consist of Oesophagostomum radiatum and Trichuris spp. In colon cattle consist of Oesophagostomum radiatum.
      PubDate: Sat, 27 Jun 2020 07:18:03 +000
      DOI: 10.20473/jops.v4i1.20272
  • Prevalensi dan Derajat Infeksi Cacing Saluran Pencernaan pada Itik Jawa
           (Anas javanica) di Dua Daerah Geografis Berbeda

    • Authors: Dian Ayu Permatasari; Kusriningrum Rochiman, Tjuk Imam Restiadi, Sri Mumpuni Sosiawati, Endang Suprihati, Mustofa Helmi Effendi
      Abstract: The aim of this study was to determined the prevalence and infection disease degree of worm gastrointestinal on duck in two different geographical territories. The duck feces examination samples were using natif method, sedimentation, and floatation. The result of this examination of lowlands showed that 20% (12/60) ducks checked that infected by two worm species of nematoda, wich ia Ascaridia galli (11,7%), Heterakis sp. (8,3%), with the range of infection degree classified as low infection (0-500 EPG), while the examination results of highlands showed that 44% (33/75) ducks checked infected by two worm species of nematoda, which is Ascaridia galli (17,3%), Heterakis sp. (10,7%) and one species of trematoda, which is Echinostoma sp. (6,7%), with the range infection degree classified low infection (0-500 EPG). it can get both single or mix infection. The analysis result has shown that there was differences between lowlands and highlands on the prevalence and infection disease degree of worm gastrointestinal on duck, was highly significant (p<0,05).
      PubDate: Sat, 27 Jun 2020 07:17:56 +000
      DOI: 10.20473/jops.v4i1.20271
  • Efektifitas Anthelmintika Ekstrak Etanol Kulit Buah Delima (Punica
           granatum) terhadap Jumlah Kematian Cacing Ascaridia gallisecara inVitro

    • Authors: Nila Murodah; Sri Mumpuni Sosiawati, Iwan Sahrial Hamid, Setiawan Koesdarto, Rochmah Kurniasanti, Poedji Hastutiek
      Abstract: This research was conducted to determine the anthelmintics effect of peel a pomegranate fruit extract etanol against Ascaridia galli worm in vitro. In this research, using 240 samples of A. galli with length 7-11cm without differentiating sex. The concentration of peel a pomegranate fruit extract etanol for immersing the Ascaridia galli were 10 mg/ml, 20 mg/ml, 40 mg/ml, and 80 mg/ml. Negative control was used PBS. Positif control was used piperazin citrate 10 mg/ml. Observation death A. galli worm and analysis of the data at the 6 hour, 12 hour, 18 hour, 24 hour, 30 hour and 36 hour using ANOVA test and Duncan Range Test. ANOVA result showed significant differences between treatments (p<0,05). Duncan Range Test result showed peel a pomegranate extract etanol 40 mg/ml had anthelmintics effects the 36 hour which comparable with piperazin citrate 10 mg/ml.
      PubDate: Sat, 27 Jun 2020 07:17:50 +000
      DOI: 10.20473/jops.v4i1.20270
  • Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2
           Toxocara Cati

    • Authors: Diyah Ayu Candra; Nunuk Dyah Retno Lastuti, Nove Hidayati, Kusnoto Kusnoto, Poedji Hastutiek, Retno Bijanti
      Abstract: The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.
      PubDate: Sat, 27 Jun 2020 07:17:43 +000
      DOI: 10.20473/jops.v4i1.20269
  • Prevalensi Ektoparasit pada Kambing Kacang di Kecamatan Prambon Kabupaten

    • Authors: Nining Sari Virgandina Vinola; Agus Sunarso, Nenny Harijani, Endang Suprihati, Poedji Hastutiek, Mufasirin Mufasirin
      Abstract: The purpose of this research are to identify and prevalence of ectoparasite infestation in bean goats on the sub-district of Prambon, district of Nganjuk. The research was conducted from September until December 2019 with 115 samples of bean goats. The samples were identified in the Entomology and Protozoology Laboratory of Parasitology Department of the Veterinary Faculty of Airlangga University. This research use non-experimental method with survey study. This research found several kind of extoparasites that infestation bean goats, as follows: Bovicola caprae 24.3% the most infestation in dorsal region, Linognatus africanus 31.2% the most infestation in head-neck and dorsal region, Ctenocepallides felis 4.3%. Mix infestation found B. Caprae and L. Africanus 20.8%, B. Caprae and C. Felis 1%, B. Caprae, L. Africanus and C. Felis 1%. This ectoparasite usually found infestatio in head-neck, ventral, dorsal, inguinal, and ectremites. The prevalence of ectoparasites is 82.6% area at sub-district of Prambon, district of Nganjuk. The data analyzed using Chi-square test and Correspondence test. Statistically is no significant association (p>0.05) between the prevalence of ectoparasites that infested bean goats with types of stage and non-stage eclosure. This caused due to environmental conditions are dirty and many other livestock around the cage.
      PubDate: Sat, 27 Jun 2020 07:17:36 +000
      DOI: 10.20473/jops.v4i1.20222
  • Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola
           Gigantica Serum by Using Western Blot Technique

    • Authors: Firman Hadi Fanani; Kusnoto Kusnoto, Poedji Hastutiek, Muchammad Yunus, Setiawan Koesdarto, Endang Suprihati
      Abstract: The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 μg/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.
      PubDate: Sat, 27 Jun 2020 07:17:27 +000
      DOI: 10.20473/jops.v4i1.20176
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