Journal Cover Jurnal Teknologi Laboratorium
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 2338-5634 - ISSN (Online) 2580-0191
   Published by Poltekkes Kemenkes Yogyakarta Homepage  [1 journal]
  • Isolation of Candida albicans from Swab Mucosa Mouth Patient Diabetes
           Mellitus Type 2

    • Authors: Ni Kadek Sri Jayanti, I Nyoman Jirna
      Pages: 1 - 7
      Abstract: Candida albicans can be pathogen when immunity had decreased and physiological function is impaired, such as in diabetes mellitus type 2. This study aims to isolation Candida albicans that collected from the oral cavity of diabetes mellitus type 2 patients. This research is conducted with a descriptive study by observing the presence of Candida albicans in 30 samples of diabetes mellitus type 2 patients, which grows on Potato Dextrose Agar. The microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours. Based on this research was found 14 (46,7%) patients from 30 patients were positive Candida albicans.
      PubDate: 2018-03-01
      Issue No: Vol. 7, No. 1 (2018)
       
  • Immunomodulator of Ethanol Extracts of The Leaves Azadirachta indica
           Against Macrophage Peritoneal Cell in Mice Induced The Vaccine BCG

    • Authors: Yogi Khoirul Abror, Evy Diah Woelansari, Suhariyadi Suhariyadi
      Pages: 8 - 14
      Abstract: This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.
      PubDate: 2018-05-03
      Issue No: Vol. 7, No. 1 (2018)
       
  • The Optimization of Secondary Metabolite Production Time and Screening
           Antibacterial Activity of Actinomycetes Isolate from Tin Plant Rizosfer
           (Ficus carica)

    • Authors: Warsi Warsi, Nanik Sulistyani
      Pages: 15 - 24
      Abstract: Some Actinomycetes isolates of tin plant (Ficus carica L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41 and T43. The aim of this study were to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin Resistant Staphyllococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.
      PubDate: 2018-05-03
      Issue No: Vol. 7, No. 1 (2018)
       
  • Potention of Antibacterial Isolat Actinomycetes to Proteolitic and
           Amilolitic Activity Escherichia Coli ATTC 25922

    • Authors: Meiskha Bahar, Fajriati Zulfa
      Pages: 25 - 30
      Abstract: Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
      PubDate: 2018-03-01
      Issue No: Vol. 7, No. 1 (2018)
       
  • The Variation of Alpha Cyclodextrin and Centrifugation Time in Lipemic
           Serum Preparation for Glucose Examination With GOD-PAP Method

    • Authors: Arfa Izzati, Ani Riyani
      Pages: 31 - 37
      Abstract: Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.
      PubDate: 2018-05-03
      Issue No: Vol. 7, No. 1 (2018)
       
 
 
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