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Journal Cover Research & Reviews : Journal of Veterinary Science and Technology
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 2349-3690 - ISSN (Online) 2319-3441
   Published by STM Journals Homepage  [22 journals]
  • Designing Mithun Specific Probes Towards Development of Microarray for
           Mithun (Bos frontalis)

    • Authors: M Mondal, M Karunakaran, KK Baruah
      Pages: 1 - 7
      Abstract: cDNA microarrays are powerful tools to analyze the expression pattern of thousands of genes simultaneously in response to treatments, during tissue differentiation or related to differences in traits. To get accurate results, the probes used in the microarray are to be specific to the species for which the array is being conducted. Non-availability of suitable microarray specific to mithun (Bos frontalis), a rare ruminant of Southeast Asia, limits its use to study the expression patterns of genes globally. Therefore, the authors designed the probes and developed a mithun-specific microarray for the first time. Transcripts obtained from bovine genome array in mithun tissues and from BLAST search were used to design probes and subsequent development of mithun-specific microarray. A total of 3,35,576 numbers of transcripts obtained from the next generation sequencing (through microarray using bovine genome array chips) of mithun tissues including several hundreds of unknown genes and others obtained for BLAST search (11,865) were used for designing the probes towards development of mithun-specific microarrays. A total of 3,47,441 probes were designed and length of each probe was 60 bp. The developed array has total features of 4,20,288, of which 4,16,505 features are readily available in the developed array. Besides, there are 3783 numbers of controls in the array to obtain high performance. In conclusion, the authors developed mithun-specific microarray using probes that are specific to this species for the first time. This microarray for mithun contains fully defined set of genes suitable for detailed analysis of expression of many genes involved in this species. The developed microarray will be of great use to analyze the molecular mechanisms controlling cell proliferation, differentiation in response to different treatments or variation in economic traits such as marbling and disease resistance in mithun. Keywords: Mithun, microarray, cDNA, transcripts, expression, genes Cite this Article
      Mondal M, Karunakaran M, Baruah KK. Designing mithun specific probes towards development of microarray for mithun (Bos frontalis). Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 1–7p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Haemato-Biochemical Changes in Staphylococcal Species Induced Mastitis in
           Mouse Model

    • Authors: P. Krishnamoorthy, M L Satyanarayana, BR Shome, Suguna Rao, H Rahman
      Pages: 8 - 14
      Abstract: In this study, haemato-biochemical changes in mouse mastitis by inoculating Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. Aureus isolated from bovine milk was studied. Blood was collected by intracardiac route at 6, 12, 24, 48, 72 and 96 h. Haematological changes noticed were: white blood cells (WBC) decreased in S. haemolyticus and S. chromogenes at 48 h; S. haemolyticus and S. aureus at 96 h. S. haemolyticus showed increased lymphocyte at 24 and 48 h and in S. aureus at 48 h. Neutrophil was decreased in S. haemolyticus and S. aureus group. Glucose increased at 6 h with three Coagulase Negative Staphylococcus (CNS) species and S. aureus. Glucose and total protein increased in S. chromogenes infected mice. Globulin levels showed decrease in S. epidermidis and S. aureus. Albumin globulin ratio showed decreasing trend in S. chromogenes and S. haemolyticus inoculated mice. Aspartate aminotransferase (AST) level increased at 6 and 12 h in all infected groups. Alanine aminotransferase increased at 12 and 48 h in S. chromogenes and S. aureus respectively. Lactate dehydrogenase (LDH) increased at 6 h in all the infected mice. Thus, three CNS species induced haemato-biochemical changes in haemoglobin, WBC, neutrophil, glucose, total protein, AST and LDH which may be used as indicators for diagnosis of CNS species mastitis in mice.  Keywords: Staphylococcus species, mouse mastitis, haemato-biochemical changesCite this Article Krishnamoorthy P, Satyanarayana ML, Shome BR, et al. Haemato-Biochemical Changes in Staphylococcal Species induced Mastitis in Mouse Model+. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 8–14p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Growth Performance and Weight Estimation of Large White Piglets Weaned at
           Different Ages

    • Authors: Peter T. Birteeb, saac O Tetteh, Abdul-Rahaman S Salifu
      Pages: 15 - 23
      Abstract: The study was carried out on 24 neonatal large white piglets at the National Livestock Breeding Station, Pong-Tamale to assess their performance when weaned at different ages and to determine the relationship between linear body measurements and the body weight of piglets in weight prediction. The piglets were randomly weaned at three selected weaning ages (4, 6 and 8 weeks) but reared under uniform conditions. Initial body weight (BW), initial body length (BL), initial chest girth (CG) and initial height at withers (HW); were measured. Weekly measurements of the parameters were taken for 20 weeks. By the 20th week, piglets weaned at 8 weeks were superior (P<0.05) by chest girth (58.14 cm) and body weight (18.43 kg) than those weaned at 6 weeks (50.50 cm and 12.79 kg) and 4 weeks (52.61 cm and 14.56 kg). No significant differences (P>0.05) were recorded in the body length and the height at withers of the selected weaned ages. The chest girth was the best predictor of body weight, yielding highest prediction accuracies of 92.3% (at 5 weeks), 76.9% (at 10 weeks), 86.1% (at 15 weeks) and 87.5% (at 20 weeks). Conclusively, large white piglets grew better when weaned at 8 weeks, and chest girth could be reliably used to predict body weight in large white grower pigs. Keywords: Swine, body measurements, linear, livestock, predictionCite this ArticleBirteeb Peter T, Tetteh Isaac O, Salifu Abdul-Rahaman S. Growth Perfor-mance and Weight Estimation of Large White Piglets Weaned at Different Ages. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 15–23p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Abscess on Right Thoracic Wall Due to Traumatic Reticulitis in a Buffalo

    • Authors: B Sudhakara Reddy, LSS Varaprasad Reddy, S Sivajothi
      Pages: 24 - 26
      Abstract: 3 year old heifer was presented to the clinic with repeated fever, anorexia and emaciation. Examination of buffalo revealed fever, tachycardia, tachypnea, ruminal atony with bloat and hot, pain full abscess on right thoracic wall. Haematology revealed erythrocytopenia, leucocytosis with more number of band cells. Buffalo was treated with inj. strepto penicillin along with supportive therapy and external application of iodex ointment over the abscess. After completion of five days of therapy abscess was ruptured and pus was oozed out. While draining the pus three inches iron long needle was came out from the abscess. Improvement was noticed after completion of therapy. Keywords: Abscess on right thoracic wall, traumatic reticulitis, buffalo, needle Cite this Article Sudhakara Reddy B, Varaprasad Reddy LSS, Sivajothi S. Abscess on Right Thoracic Wall due to Traumatic Reticulitis in a Buffalo. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 24–26p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Mammary Gland Fibroadenoma in a Bitch

    • Authors: Sanjiv kumar, Ramesh Tiwary
      Pages: 27 - 28
      Abstract: he work was conducted on an adult German shepherd bitch having a growing lump in the mammary gland. Tentatively it appeared to be a case of benign tumour of first left thoracic mammary gland. Sample was collected for cytopathological and histopathological examination of the affected tissue. FNAC was done by Papanicoloau’s stain while routine paraffin embedding technique was employed for histopathological examination, which revealed it to be a case of mammary gland fibroadenoma which was then surgically removed successfully with full recovery. Keywords: Mammary gland fibroadenoma, benign tumour, FNACCite this Article Sanjiv Kumar, Ramesh Tiwary. Mammary Gland Fibroadenoma in a Bitch.Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 27–28p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Development of A Rapid Microtiterplate Based Colorimetric Method for
           Estimation of Non-esterified Fatty Acids in Bovine Plasma

    • Authors: M Mondal, KK Baruah
      Pages: 29 - 34
      Abstract: Non-esterified fatty acid (NEFA) is one of the most important blood metabolites contributing significantly during body growth, cyclicity, pregnancy and lactation, and it plays more vital role particularly during transition phase of the biological processes like pregnancy to lactation transition period. Determination of blood concentrations of NEFA is, therefore, important. The measurement technique or assay for measurement of blood NEFA should be simple, reliable, accurate, rapid and cheap. Here, the authors developed a sensitive and fast microtiterplate colorimetry-based method for estimation of NEFA in bovine plasma. The method is simple to perform and gives accurate, precise results in 100 µL samples. The developed method requires at least five times less quantity of plasma and reagents than that of already existing methods. Furthermore, ability of the method for taking reading of all the samples together on microtiterplate in a reader enable us to save a lot of time. Hence, the developed method may be adopted for estimation of NEFA in bovine plasma.  Keywords: NEFA, bovine, microtiterplate, plasma, assay Cite this ArticleMondal M,BaruahKK. Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-Esterified Fatty Acids in Bovine Plasma. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 29–34p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Development of an Endocrine Profiles Based Mathematical Model for the
           Estrous Cycle in Indian Crossbred CowsDevelopment of an Endocrine Profiles
           Based Mathematical Model for the Estrous Cycle in Indian Crossbred Cows

    • Authors: M Mondal, M Karunakaran, MK Ghosh, TK Dutta
      Pages: 35 - 38
      Abstract: Declined fertility of the high-yielding dairy cows reared under intensive condition of management is of great concern not only for India but for the whole world. The bovine estrous cycle, particularly the estrus, is tightly controlled by the complex interplay of various organs and hormones. Mathematical modeling of the bovine estrous cycle could help in understanding the dynamics of this complex biological system. The authors, therefore, developed a mechanistic mathematical model for the estrous cycle of Indian crossbred cows that exclusively includes the key hormones that interact to control these processes. The authors used the data generated by them for approximately 163 estrous cycles having 1793 readings related to different hormones like LH, FSH, progesterone, estrogen, etc., under the control of the hypothalamo-pituitary-gonadal (ovarian) axis. The developed model was capable enough to generate successive estrous cycles of 21-day length. The model contains seven differential equations and 34 parameters. By simulating the developed model, a set of equations and parameters that were obtained were found to describe vividly the system consistent with empirical knowledge. In conclusion, the authors for the first time developed a mechanistic mathematical model of the estrous cycle for the Indian crossbred cows and it can be used for predicting hormonal inter milieu of the complex biological system without spending much time and money towards laboratory estimation. The developed model may also be used as a basis for more elaborate models that can predict the effects of external manipulations and genetic differences in Indian bovines. Keywords: Indian crossbred cow, hormones, differential equations, systems biology Cite this ArticleMondal M,KarunakaranM, Ghosh MK et al. Development of an Endocrine Profiles Based Mathematical Model for The Estrous Cycle in Indian Crossbred Cows. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 35–38p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
  • Diagnosis of Subclinical Mastitis in Cross Bred Cattle

    • Authors: B. SomaSekhar Reddy, B Shobhamani, B Sreedevi, K Nalini Kumari, Y Ravindra Reddy
      Pages: 39 - 43
      Abstract: Subclinical mastitis was diagnosed in 1599 quarter milk samples from 417 apparently healthy lactating cows in and around Tirupati. Diagnosis of subclinical mastitis was done by California Mastitis Test and Electrical Conductivity Test. The quarter-wise incidence of SCM as detected by CMT and EC was 27.83 and 19.95% respectively whereas the animal-wise incidence was 52.28 and 38.13% for CMT and EC respectively in the present study. Keywords: Cattle, mastitis, CMT, MWT, EC, SCCCite this ArticleSoma Sekhar ReddyB, Shobhamani B, Sreedevi B et al. Diagnosis of subclinical mastitis in cross bred cattle. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 39–43p.
      PubDate: 2018-04-21
      Issue No: Vol. 4, No. 3 (2018)
       
 
 
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