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Journal Cover Biomedical Research and Therapy
  [1 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
   Published by Vietnam National University Homepage  [1 journal]
  • CORRECTION: Isolation of endothelial progenitor cells from human adipose

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Hoa Trong Nguyen, Ngoc Kim Phan
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
  • Re-Emergence of Congo Virus in Pakistan: Call for Preparedness

    • Authors: Tauseef Ahmad, Muhmmad Khan, Saqib Malik
      First page: 742
      Abstract: Crimean-congo hemorrhagic fever (CCHF) once again re-emerged in Pakistan. In July 2016, 2 CCHF cases were reported from Lodhran and Bahawalpur districts of Pakistan. Later on the CCHF virus was also reported from other region of the country including Balochistan, Karachi and Khyber Pakhtunkhwa. Up to 22 August 2016, a total of 20 deaths were reported of which 12 from Balochistan, 5 from Karachi, 2 from Bahawalpur and 1 from Khyber Pakhtunkhwa. Precautions measurements and awareness is necessary to protect the normal individuals away from this fatal disease. The media, health department and government need to play their active role to stop the spread of CCHF in the country.
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
  • Liquid biopsies: tumor diagnosis and treatment monitoring

    • Authors: Binh Thanh Vu, Dat Tan Le, Phuc Van Pham
      First page: 745
      Abstract: Cancer is a disease with high evolutionary, i.e., malignant, characteristics that change under selective pressure from therapy. Characterization based on molecular or primary tumor properties or clinicopathological staging does not fully reflect the state of cancer, especially when cancer cells metastasize. This is the major reason for failure of cancer treatment. Currently, there is an urgent need for new approaches that allow more effective, but less invasive, monitoring of cancer status, thereby improving the efficacy of treatments. With recent technological advances, “liquid biopsies,” the isolation of intact cells or analysis of components that are secreted from cells, such as nucleic acids or exosomes, could be implemented easily. This approach would facilitate real-time monitoring and accurate measurement of critical biomarkers. In this review, we summarize the recent progress in the identification of circulating tumor cells using new high-resolution approaches and discuss new circulating tumor nucleic acid- and exosome-based approaches. The information obtained through liquid biopsies could be used to gain a better understanding of cancer cell invasiveness and metastatic competence, which would then benefit translational applications such as personalized medicine.
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
  • Optimizing a multiplex high resolution melting curve to diagnose G6PD
           deficiency based on viangchan and canton mutations

    • Authors: Nghia Le Tri, Giang Thanh Nguyen-Dien, Anh Thi Lan Dang, Ngoc Tran Bao, Hien Tran Tinh, Hue Thi Nguyen
      First page: 757
      Abstract: Glucose-6-phosphate dehydrogenase (G6PD) deficiency which is caused by mutation on G6PD gene  is the most common enzyme disorder in human. There have been 184 discovered mutations among which Viangchan [Val291Met] and Canton mutation [Arg459Leu] are the most common variants in Vietnamese. Due to the severity of this disease, several methods  have been devised for diagnostics. However, time-consuming, low sensitivity and expensiveness are major problems of those techniques. Recently, High Resolution Melting (HRM) has been developed and proven to be an effective method for DNA genotyping, mutation scanning and sequence matching. Hence, in this study a multiplex HRM has been developed aiming at detecting these two mutations concurrently. At first, a singleplex HRM was designed for each mutation. Then, conditions for these singleplex assay were combined and optimized again in order to get the optimal condition for multiplex HRM.  Although this method showed a promising potential with a high accuracy, sensitivity, and specificity, it is impractial to continue developing this method because of the lack of controls. However, the optimized singleplex PCR-HRM in this study still can be used for single mutation detection and serve as the background to develop PCR-HRM for other G6PD mutations in Vietnamese-Kinh population.
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
  • Human adipose-derived mesenchymal stem cell could participate in
           angiogenesis in a mouse model of acute hindlimb ischemia

    • Authors: Thuy Thi-Thanh Dao, Ngoc Bich Vu, Lan Thi Phi, Ha Thi -Ngan Le, Ngoc Kim Phan, Van Thanh Ta, Phuc Van Pham
      First page: 770
      Abstract: Mesenchymal stem cells (MSCs) transplantation for the treatment of acute hindlimb ischemia is recently attracting the attention of many scientists. Identifying the role of donor cells in the host is a crucial factor for improving the efficiency of treatment. This study evaluated the injury repair role of xenogeneic adipose-derived stem cell (ADSC) transplantation in acute hindlimb ischemia mouse model. Human ADSCs were transplanted into the limb of ischemic mouse. The survival rate of grafted cells and expression of human VEGF-R2 and CD31 positive cells were assessed in the mouse. In addition, the morphological and functional recovery of ischemic hindlimb was also assessed. The results showed that one-day post cell transplantation, the survival percentage of grafted cells was 3.62% ± 2.06% at the injection site and 15.71% ± 12.29% around the injection site. The rate of VEGFR2-positive cells had highest expression at 4 days post transplantation, approximately 5.46% ± 2.13% at the injection site; 9.12% ± 7.17% at the opposite of injection site, and 7.22% ± 4.59% at the lateral gastrocnemius. The percentage of CD31 positive cells increased on day 4 at the injection site to approximately 0.8% ± 1.60%, and further increased on day 8 at the lateral gastrocnemius site and the opposite injection site to approximately 1.56% ± 0.44% and 1.17% ± 1.69%, respectively. After 14 days, the cell presentation and the angiogenesis marker expression were decreased to zero, except for CD31 expression at the opposite of injection site (0.72% ± 1.03%). Histological structure of the cell-injected muscle tissue remained stable as that of the normal muscle. New small blood vessels were found growing in hindlimb. On the other hand, approximately 66.67% of mice were fully recovered from ischemic hindlimb at grade 0 and I after cell injection. Thus, xenotransplantation of human ADSCs might play a significant role in the formation of new blood vessel and can assist in the treatment of mouse with acute hindlimb ischemia.
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
  • Direct reprogramming of fibroblasts into endothelial progenitor cells by
           defined factors

    • Authors: Mai Thi-Hoang Truong, Oanh Thuy Huynh, Liem Hieu Pham, Phuc Van Pham
      First page: 780
      Abstract: Endothelial progenitor cells (EPCs) are important progenitor cells in vasculargenesis as well as in tissue engineering. However, a few EPCs can be isolated from bone marrow, peripheral blood and umbilical cord blood. Moreover, in vitro their proliferation potential also is limited. Therefore, this study aimed to produce EPCs from direct reprogramming of fibroblasts by transduction with some specific factors. Human fibroblasts were collected from the human skin by published protocols. These cells were transduced with 2 viral vectors containing 5 factors, included Oct3/4, Sox2, Klf4, c-Myc (plasmid 1), VEGFR2 (plasmid 2). Transduced cells were treated with the endothelial cell medium for 21 days. These cells were analyzed the expression of Oct3/4, Sox3, Klf4, c-Myc and VEGFR2 at day 5, and EPC phenotype at day 21. The results showed that after 5 days of transduction, fibroblasts acquired partial pluripotential. After 21 days of transduction and cultured in the endothelial cell medium, these cells exhibited the endothelial markers included CD31 and VEGFR2, formed blood vessels like capillary. Our finding suggested another strategy for direct reprogramming of fibroblasts into EPCs.
      PubDate: 2016-08-30
      Issue No: Vol. 3, No. 8 (2016)
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