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Biomedical Research and Therapy
   [4 followers]  Follow    
  This is an Open Access Journal Open Access journal
     ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
     Published by Vietnam National University Homepage  [1 journal]
  • Castleman's disease of the mesocolon: a rare case report

    • Authors: Mythri Mahesh Boovalli, Kalyani Raju, Srinivas Murthy Venkataramappa
      Abstract: Castleman’s disease is a rare form of localized lymph node hyperplasia of unknown etiology. The sub-types are; hyaline vascular, plasma cell and mixed variant. Clinical subtypes are localized (unicentric) and multicentric. It is reported in all age groups regardless of gender. Hyaline vascular type, accounts for 90% of all cases, often develops in the neck, mediastinum and pulmonary hilum. Its occurrence in the peritoneal cavity is very rare. We present a case in mesocolon of hyaline type in a 39 year female.
      PubDate: 2014-07-24
      Issue No: Vol. 1, No. 3 (2014)
  • Good manufacturing practice-compliant isolation and culture of human
           adipose derived stem cells

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Nhan Lu-Chinh Phan, Dung Minh Le, Nhat Chau Truong, Nhung Hai Truong, Khanh Hong-Thien Bui, Ngoc Kim Phan
      Abstract: Adipose-derived stem cells (ADSCs) are wonderful cells for regenerative medicine. Like mesenchymal stem cells, ADSCs hold multi-potent differentiation capacity that can differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into some other cells. ADSC transplantation rapidly increased for recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 10th passage could not form tumors in NOD/SCID mice. This research contributed a suitable protocol for clinical applications of expanded ADSCs.
      PubDate: 2014-07-05
      Issue No: Vol. 1, No. 3 (2014)
  • Direct reprogramming of somatic cells: an update

    • Authors: Phuc Van Pham
      Abstract:             Direct reprogramming is a technique that converting an adult cell into another differentiated cells – such from fibroblasts into cardiomyocytes – without passage through an undifferentiated pluripotent stage. This is a novel technology that opening a new chance for both biological research and regenerative medicine. Some preliminary studies about direct reprogramming started in the 1980s when differentiated adult cells could be converted into another differentiated cells by over-expressing some transcription factor genes. These studies showed that differentiated cells or mature cells also have the plasticity. To date, direct reprogramming becomes a powerful tool in the biological research and regenerative medicine, especially personalized medicine. Here, this review aimed to summarize all direct reprogramming studies of somatic cells by master control genes into others as well as potential applications of these techniques in research and treatment of some human diseases.
      PubDate: 2014-06-28
      Issue No: Vol. 1, No. 3 (2014)
  • Preliminary evaluation of intravenous infusion and intra-pancreatic
           injection of human umbilical cord blood-derived mesenchymal stem cells for
           the treatment of diabetic mice

    • Authors: Ngoc Kim Phan, Thuy Thanh Duong, Truc Le-Buu Pham, Loan Thi-Tung Dang, Anh Nguyen-Tu Bui, Vuong Minh Pham, Nhat Chau Truong, Phuc Van Pham
      Abstract:  Type 1 diabetes mellitus is characterized by the destruction of pancreatic islet beta cells, which leads to insulin insufficiency, hyperglycemia, and reduced metabolic glucose level. Insulin replacement is the current standard therapy for type 1 diabetes mellitus but has several limitations. Pancreatic islet transplantation can result in the production of exogenous insulin, but its use is limited by immune-rejection and donor availability. Recent studies have shown that mesenchymal stem cells (MSCs) can trans-differentiate into insulin-producing cells (IPCs), which could be utilized for diabetes mellitus treatment. Previously published reports have demonstrated that MSC or IPC transplantation could produce significant improvement in mouse models of diabetes mellitus. This study was aimed at determining the effects of two different methods of MSC transplantation on the efficacy of diabetes mellitus treatment in mouse models. The MSCs were isolated from umbilical cord blood and were proliferated following a previously published procedure. Diabetes mellitus was induced in mice by streptozotocin (STZ) injection. Thirty days after transplantation, the weight of the mice treated by intra-venous infusion and intra-pancreatic injection was found to be 22% and 14% higher than that of the un-treated mice. The blood glucose concentrations in both intra-venous infusion and intra-pancreatic injection groups decreased and remained more stable than those in the control group. Moreover, insulin was detected in the serum of the treated mice, and the pancreas also showed gradual recovery. Based on the results of this preliminary investigation, intra-venous infusion seems more suitable than intra-pancreatic injection for MSC transplantation for diabetes mellitus treatment. 
      PubDate: 2014-06-23
      Issue No: Vol. 1, No. 3 (2014)
  • Oncolytic virotherapy for cancer

    • Authors: Kotovoe Erlomeava
      Abstract: Cancer is the leading caused of death in human. In recent years, there are some therapies developed in the diagnosis and treatment of cancers. However, overall outcome is not efficiently improved. Virotherapy using oncolytic viruses is considered as novel strategy for cancer treatment. In principle, virotherapy used oncolytic viruses that can infect and lyse target cancer cells. Because oncolytic viruses can specific infect to target cells through receptors in cell surface. So this therapy promises without damage with healthy tissue. This review aimed to describe the use of oncolytic viruses in cancer treatment in recent years.
      PubDate: 2014-06-13
      Issue No: Vol. 1, No. 3 (2014)
  • Breast cancer tumor growth is efficiently inhibited by dendritic cell
           transfusion in murine model

    • Authors: Viet Quoc Pham, Sinh Truong Nguyen, Trang Thi Mai, Ngoc Kim Phan, Phuc Van Pham
      Abstract: The ability of dendritic cells to efficiently present tumor-derived antigens when primed with tumor cell lysates makes them attractive as an approach for cancer treatment. This study aimed to evaluate the effects of dendritic cell transfusion dose on breast cancer tumor growth in a murine model. Dendritic cells were produced from allogeneic bone marrow-derived mononuclear cells that were cultured in RPMI 1640 medium supplemented with 20 ng/mL GMCSF and 20 ng/mL IL-4 for 7 days. These cells were checked for maturation before being primed with a cancer cell-derived antigen. Cancer cell antigens were produced by a rapid freeze-thaw procedure using a 4T1 cell line. Immature dendritic cells were loaded with 4T1 cell-derived antigens. Dendritic cells were transfused into mice bearing tumors at three different doses, included 5.104, 105, and 106 cells/mouse with a control consisting of RPMI 1640 media alone. The results showed that dendritic cell therapy inhibited breast cancer tumors in a murine model; however, this effect depended on dendritic cell dose. After 17 days, in the treated groups, tumor size decreased by 43%, 50%, and 87.5% for the doses of 5 × 104, 105, and 106 dendritic cells, respectively, while tumor size in the control group decreased by 44%. This result demonstrated that dendritic cell therapy is a promising therapy for breast cancer treatment.
      PubDate: 2014-06-04
      Issue No: Vol. 1, No. 3 (2014)
  • Micronucleus Assessment as a Biomarker and Susceptibility to DNA Damage in
           Workers Occupationally Exposed to Pesticides

    • Authors: Mohammed RafiqKhan, Godan Tharangad Krishnan, Ranjini Keezhekalam, Srinivasapuram Natarajan Suresh, Umadevi Pongiya, Yalaga Rama Rao
      Abstract: Occupational exposures to hazardous chemicals are common in industries using solvent based materials as well as in indoor environments where people are exposed to volatile organic compounds from various sources. The health of the workers has several determinants, including risk factors at the workplace leading to cancer. The aim of the present investigation was to assess the potential cytogenetic damage associated with occupational exposure among pesticide workers by using micronuclei and other nuclear abnormalities as a biomarker. The micronucleus assay on exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. To determine the genotoxic effects of pesticide workers, Micronucleus assay was carried out in exfoliated buccal cells of 50 pesticide workers and 50 controls. For each individual, 2,000 exfoliated buccal cells were analyzed. Micronucleus and other nuclear abnormalities frequencies in exposed were significantly higher than those in control groups (P < 0.05) and also significantly related to smoking, tobacco chewing and alcohol drinking habit (P < 0.05). Increased frequency of these nuclear abnormalities in buccal epithelial cells of exposed workers indicates adverse cellular reaction and/or a surveillance mechanism to eliminate cells with genetic damage. The present studied individuals may be at a higher risk of developing cancer and therefore monitored for any long term adverse effects of the exposure. Genotoxic studies are foremost for any occupational exposure studies. Evaluation based on genotoxic parameters is often useful in warranting environmental endowment and occupational health. Genotoxicity biomarkers have received a considerable interest as tools for detecting human genotoxic exposure and effects, especially in health surveillance programs dealing with chemical carcinogens.
      PubDate: 2014-06-01
      Issue No: Vol. 1, No. 3 (2014)
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