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Journal Cover   Biomedical Research and Therapy
  [4 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
   Published by Vietnam National University Homepage  [1 journal]
  • In vitro anti-oxidant and in vivo anti-inflammatory activity determination
           of the methanolic leaves extract of Millettia pachycarpa

    • Authors: Shofiul Azam, Prawej Ansari, Mohammad Mamun-Ur Rashid, Mohammad Nazmul Alam, Ismail Hussein Ahmed, Mubarik Yusuf Ibarahim, Shah Mohammed Shafi, Sadequr Rahman, Altaf Hossen
      Abstract: Objective: Millettia pachycarpa is a member of the Fabacea family and our object has been set to examine the anti-oxidant and anti-inflammatory endeavor of this plant. This plant has been reported as potential anti-inflammatory agent previously.Materials and Method: DPPH free radical scavenging and reducing power assay was carried out to assess the electron donating capability of the methanolic crude extract of the specimen. A quantitative analysis has been made to measure phenolic and flavonoid content. The anti-inflammatory potentiality of methanolic extract of M. pachycarpa has been determined via carrageenan-induced paw edema assay in rats.Results: The IC50 value estimated was, 196.47 ± 2.061 µg/ml, which is a very much promising result. From quantitative assessment, sample extract reported very good number of phenolic and flavonoid measurement, 41.23 ± 1.527 mg equivalent gallic acid per gram and 110.33 ± 2.255 mg equivalent rutin per gram, respectively. The studied specimen showed expected dose dependent result in anti-inflammatory assay. At the dose of 300 mg/kg the extract showed maximum inhibitory effect (61.36% at 2 hour; P <0.001) on paw swelling due to Carrageenan injection.Conclusion: This finding have proved that methanolic extract of M. pachycarpa possess potent anti-inflammatory activity possibly due to presence of good quantity flavonoid and phenolic content, also have good anti-oxidant property.
      PubDate: 2015-09-20
      Issue No: Vol. 2, No. 9 (2015)

    • Authors: Nataliia Raksha, Dmitrii Gladun, Oleksii Savchuk, Lydmila Ostapchenko
      First page: 354
      Abstract: Marine organisms have been recognized as rich sources of bioactive compounds with valuable biotechnology potential. Enzymes extracted from marine hydrobionts have gained much attention because of their unique quite specific properties that determined their profound applications in chemical, medical, food industries and molecular biology experiments. In this regard, our work focused on investigation of proteolytic potential of marine hydrobionts. At first, tissue extract of Antarctic hydrobiont Parborlasia corrugatus was separated by gel filtration chromatography on a Superdex-75 PG. Further zymography with using gelatin as substrate revealed the presence of clear band that can indicate about active enzymes. It had been shown the presence of collagenolytic activity in all eight fractions obtained after chromatographic separation of tissue extract. Trypsin-like (L-BApNA hydrolyzing) was found only in first fraction. Our results let us assume that P. corrugatus can be regarded as potential source of enzymes for practical use.
      PubDate: 2015-09-05
      Issue No: Vol. 2, No. 9 (2015)
  • Evaluation in vitro senescence of mesenchymal stem cells isolated from
           mouse bone marrow and adipose tissue

    • Authors: Sinai Walker, Gene Sam, Martin Hill, Matthie Robert
      Abstract: Mesenchymal stem cells (MSCs) from bone marrow and adipose tissue are intensively used for regenerative medicine. This study aimed to compare the in vitro senescence of MSCs isolated from adipose (BM-MSCs) and bone marrow tissues (ADSCs). Both BM-MSCs and ADSCs were isolated from the same mice, and sub-cultured to 3rd passage. These MSCs were assessed cell proliferation, senescence associated beta-galactosidase staining, and telomere length as well as stemness marker expression. The results showed that both BM-MSCs and ADSCs would go to senescence after long-term culture. BM-MSCs significantly decreased the proliferation rate after passage 20, while ADSCs could strongly proliferate to passage 50. Based on beta-galactosidase expression, BM-MSCs also were positive sooner than ADSCs (passage 10 vs. passage 20, respectively). At these passages, both BM-MSCs and ADSCs exhibited the shortening of telomere length from 10.4 to 5.2 kbps in passage 3 to senescent cultures, respectively. In conclusion, MSCs from bone marrow appear to senesce much earlier than those from adipose tissue. These results suggested that numbers of sub-culture should be controlled to get MSCs in good status for research or application.
      PubDate: 2015-08-01
      Issue No: Vol. 2, No. 8 (2015)
  • Retinoic acid improves the cisplatin effect on tumor initiating cells in
           liver cancer

    • Authors: Julie Arnold, William Hwang, Sudipto Bari, Hong Zhang
      Abstract: In recent years, cisplatin is used in hepatocellular carcinoma (HCC) treatment as adjuvant treatment for post-operation patients. However, its efficiency also was largely limited because of the high incidence of chemoresistance. It was the aim of this study to assess the effects of cisplatin in combination with retinoic acid (RA) on differentiation and apoptosis of tumor-initiating cells (TICs) in human HCC. In this study, TICs would be treated cisplatin+RA and cisplatin or RA alone as the controls. The TIC differentiation was evaluated by phenotype changes while TIC apoptosis was evaluated by PI-annexin V assay. The results showed that RA effectively induced differentiation of TICs, which potentiated the cytotoxic effects of cisplatin. The combinatorial treatment of RA and cisplatin significantly promoted apoptosis, and differentiation of human TICs compared to the treatment with either drug alone. These findings demonstrated that the combination of RA with cisplatin may be a promising strategy in HCC treatment.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • Characterization and expression of pluripotency markers in human dental
           pulp stem cells

    • Authors: Miglino Maria, Laurino Carlos, Rosalia Mendez-Otero, Natasha Machado
      Abstract: Human dental pulp stem cell (DPSC) is new source of stem cells. In this study, we characterized expression of pluripotency markers such as Oct-3/4, Sox-2, and Nanog in DPSCs. DPSCs were isolated according to the published procedure. They were sub-cultured to 3rd passage and used for further experiments. Firstly, DPSCs were characterized with mesenchymal stem cell phenotypes included expression of CD29, CD44, CD73, CD90 and CD105; adipogenesis and osteogenesis differentiation. Secondly, DPSCs were checked expression of Oct-3/4, Sox-2, and Nanog by both RT-PCR, immunofluorescence. The results showed that DPSCs satisfied the minimal criteria of MSCs. Moreover, they also expressed the pluripotent markers Oct-3/4, Sox-2, and Nanog at both mRNA and protein level. Thus, these factors may have a potential role in influencing the high proliferation and differentiation of DPSCs. In conclusion, this result showed that DPSCs represent as autologous stem cell source with high potential for dental engineering as well as regenerative medicine.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • Hypoxia condition promoted the adipose derived stem cell proliferation via
           VEGF production

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Nhung Hai Truong, Loan Thi-Tung Dang, Nhan Lu-Chinh Phan, Ngoc Kim Phan
      Abstract: Adipose-derived stem cells (ADSCs) are a promising mesenchymal stem cells source with therapeutic applications. Some recent studies showed that ADSCs could be expanded in vitro without phenotype changes. This study aimed to evaluate the effect of hypoxia condition on ADSC proliferation in vitro and to determine the role of VEGF in ADSC proliferation. ADSCs were selectively cultured from stromal vascular fraction that obtained from adipose tissue in the DMEM/F12 medium supplemented with 10% FBS, 1% antibiotic-antimycotic. ADSCs were cultured in two conditions included hypoxia (5% O2) and normal oxygen (21% O2). Effects of oxygen concentration on cell proliferation were recorded by cell cycle and doubling time. Expression of VEGF was evaluated by real-time RT-PCR and ELISA assays. And the role of VEGF on ADSC proliferation was studied by neutralizing VEGF with anti-VEGF monoclonal antibody. The results showed that ADSC proliferation rate was increased 2.5 times in hypoxia compared to normal oxygen. And hypoxia condition, ADSCs also triggered VEGF synthesis at both mRNA and translational level. However, neutralizing VEGF with anti-VEGF monoclonal antibody significantly reduced the proliferation rate. These results suggested that hypoxia stimulated ADSC proliferation related to VEGF production. This finding will contribute not only in stem cell technology but also in obese treatment.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • A safety evaluation of adipose derived stem cells

    • Authors: Ngoc Bich Vu, Van Ngoc Le Trinh, Lan Thi Phi, Trieu Le Hoang Vo, Thuy Thi Thanh Dao, Ngoc Kim Phan, Van Thanh Ta, Phuc Van Pham
      First page: 359
      Abstract: Adipose tissue contains a large numbers of stem cells. Adipose-derived stem cells (ADSC) employed in regenerative medicine. This study aimed to isolate ADSC and evaluate the safety of ADSC in mice model. Stromal vascular fraction (SVF) was collected from adipose tissue by collagenase. Then, ADSCs were isolated from SVFs by in vitro culture. ADSCs were evaluated their stemness in vitro by self-renewal potential, differentiation potential into osteoblasts, adipocytes, and chondroblasts as well as expression of some specific markers. Finally, the ADSCs were evaluated tumor formation in vivo in athymic mouse. Results showed that 100% samples of ADSCs were developed well and maintained homogeneous up to passage 10th. ADSCs were completely sterilized and could not cause tumors in athymic mouse. These initial results showed that ADSCs were safety for stem cell therapy.
      PubDate: 2015-08-02
      Issue No: Vol. 2, No. 8 (2015)
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