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Journal Cover   Biomedical Research and Therapy
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
   Published by Vietnam National University Homepage  [1 journal]
  • Evaluation in vitro senescence of mesenchymal stem cells isolated from
           mouse bone marrow and adipose tissue

    • Authors: Sinai Walker, Gene Sam, Martin Hill, Matthie Robert
      Abstract: Mesenchymal stem cells (MSCs) from bone marrow and adipose tissue are intensively used for regenerative medicine. This study aimed to compare the in vitro senescence of MSCs isolated from adipose (BM-MSCs) and bone marrow tissues (ADSCs). Both BM-MSCs and ADSCs were isolated from the same mice, and sub-cultured to 3rd passage. These MSCs were assessed cell proliferation, senescence associated beta-galactosidase staining, and telomere length as well as stemness marker expression. The results showed that both BM-MSCs and ADSCs would go to senescence after long-term culture. BM-MSCs significantly decreased the proliferation rate after passage 20, while ADSCs could strongly proliferate to passage 50. Based on beta-galactosidase expression, BM-MSCs also were positive sooner than ADSCs (passage 10 vs. passage 20, respectively). At these passages, both BM-MSCs and ADSCs exhibited the shortening of telomere length from 10.4 to 5.2 kbps in passage 3 to senescent cultures, respectively. In conclusion, MSCs from bone marrow appear to senesce much earlier than those from adipose tissue. These results suggested that numbers of sub-culture should be controlled to get MSCs in good status for research or application.
      PubDate: 2015-08-01
      Issue No: Vol. 2, No. 8 (2015)
  • Retinoic acid improves the cisplatin effect on tumor initiating cells in
           liver cancer

    • Authors: Julie Arnold, William Hwang, Sudipto Bari, Hong Zhang
      Abstract: In recent years, cisplatin is used in hepatocellular carcinoma (HCC) treatment as adjuvant treatment for post-operation patients. However, its efficiency also was largely limited because of the high incidence of chemoresistance. It was the aim of this study to assess the effects of cisplatin in combination with retinoic acid (RA) on differentiation and apoptosis of tumor-initiating cells (TICs) in human HCC. In this study, TICs would be treated cisplatin+RA and cisplatin or RA alone as the controls. The TIC differentiation was evaluated by phenotype changes while TIC apoptosis was evaluated by PI-annexin V assay. The results showed that RA effectively induced differentiation of TICs, which potentiated the cytotoxic effects of cisplatin. The combinatorial treatment of RA and cisplatin significantly promoted apoptosis, and differentiation of human TICs compared to the treatment with either drug alone. These findings demonstrated that the combination of RA with cisplatin may be a promising strategy in HCC treatment.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • Characterization and expression of pluripotency markers in human dental
           pulp stem cells

    • Authors: Miglino Maria, Laurino Carlos, Rosalia Mendez-Otero, Natasha Machado
      Abstract: Human dental pulp stem cell (DPSC) is new source of stem cells. In this study, we characterized expression of pluripotency markers such as Oct-3/4, Sox-2, and Nanog in DPSCs. DPSCs were isolated according to the published procedure. They were sub-cultured to 3rd passage and used for further experiments. Firstly, DPSCs were characterized with mesenchymal stem cell phenotypes included expression of CD29, CD44, CD73, CD90 and CD105; adipogenesis and osteogenesis differentiation. Secondly, DPSCs were checked expression of Oct-3/4, Sox-2, and Nanog by both RT-PCR, immunofluorescence. The results showed that DPSCs satisfied the minimal criteria of MSCs. Moreover, they also expressed the pluripotent markers Oct-3/4, Sox-2, and Nanog at both mRNA and protein level. Thus, these factors may have a potential role in influencing the high proliferation and differentiation of DPSCs. In conclusion, this result showed that DPSCs represent as autologous stem cell source with high potential for dental engineering as well as regenerative medicine.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • Hypoxia condition promoted the adipose derived stem cell proliferation via
           VEGF production

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Nhung Hai Truong, Loan Thi-Tung Dang, Nhan Lu-Chinh Phan, Ngoc Kim Phan
      Abstract: Adipose-derived stem cells (ADSCs) are a promising mesenchymal stem cells source with therapeutic applications. Some recent studies showed that ADSCs could be expanded in vitro without phenotype changes. This study aimed to evaluate the effect of hypoxia condition on ADSC proliferation in vitro and to determine the role of VEGF in ADSC proliferation. ADSCs were selectively cultured from stromal vascular fraction that obtained from adipose tissue in the DMEM/F12 medium supplemented with 10% FBS, 1% antibiotic-antimycotic. ADSCs were cultured in two conditions included hypoxia (5% O2) and normal oxygen (21% O2). Effects of oxygen concentration on cell proliferation were recorded by cell cycle and doubling time. Expression of VEGF was evaluated by real-time RT-PCR and ELISA assays. And the role of VEGF on ADSC proliferation was studied by neutralizing VEGF with anti-VEGF monoclonal antibody. The results showed that ADSC proliferation rate was increased 2.5 times in hypoxia compared to normal oxygen. And hypoxia condition, ADSCs also triggered VEGF synthesis at both mRNA and translational level. However, neutralizing VEGF with anti-VEGF monoclonal antibody significantly reduced the proliferation rate. These results suggested that hypoxia stimulated ADSC proliferation related to VEGF production. This finding will contribute not only in stem cell technology but also in obese treatment.
      PubDate: 2015-07-01
      Issue No: Vol. 2, No. 8 (2015)
  • Ethanopharmacological study of the aqueous, chloroform, ethanol leaves
           extracts and latex of Calotropisprocera in diabetic rats

    • Authors: Ali Ismaiel Ali-Abd Alrheam, Zafer Saad-Al Shehri
      Abstract: Introduction: Calotropisprocera is a member of the plant family Asclepiadaceae, a shrub about 6m high and is widely distributed in the kingdom of Saudi Arabia. This study aimed to show some medicinal potentials and biological activity of Calotropisprocera and to discover new natural, safe and effective materials available in the Saudi Arabia environment. Marerial and Methods:The leaves extracts and latex of Calotropisprocerawere investigated for its anti-hyperglycemic effect in Male Wister Albino rats. Diabetes was induced by administration of single dose of streptozotocin (STZ, 50 mg/kg, I.P). Forty two male albino rats, weighting 150-200 gm divided into seven groups, each consisted of  6 rats as follows: Group I : Normal control, Group II: Diabetic control, Group III: Diabetic rats given Glibenclamide 600 μg/kg, Group IV: Diabetic rats given aqueous leaves extracts C. procera200mg/kg b. wt, Group V: Diabetic rats given chloroform leaves extracts C. procera200mg/kg b. wt, Group VI: Diabetic rats given ethanol leaves extracts C. procera200mg/kg b. wt, Group VII: Diabetic rats given latex of C. procera200mg/kg b. wt.The leaves extracts and latex of Calotropisprocera were administered as single dose per day to diabetes-induced rats for a period of 15 days.The effect of C. proceraon blood glucose level was measured in the diabetic rats. Serum lipid profile (Total cholesterol, triglycerides, low density, and high density lipoprotein) also were measured. The activities were also compared to that effect produced by a standard anti-diabetic agent, glibenclamide 500μg/kg. Results and Discussion:The results showed a significant decrease in the mean level of blood glucose and serum cholesterol, Triglycrides, HDL, LDL. Calotropisprocera appears to be a rich source of phytoconstituents that activate and inhence a pharmacological response of different parts of the body and this study need further studies to shows the complete properties of the plant.
      PubDate: 2015-11-23
      Issue No: Vol. 2, No. 11 (2015)
  • Production of dendritic cells and cytokine-induced killer cells from
           banked umbilical cord blood samples

    • Authors: Phuc Van Pham, Binh Thanh Vu, Viet Quoc Pham, Phong Minh Le, Hanh Thi Le, Ngoc Kim Phan
      Abstract: Umbilical cord blood (UCB) is considered to be a source of hematopoietic stem cells (HSCs). All UCB banks have recently become interested in the isolation and storage of HSCs for the treatment of hematological diseases. However, UCB was also recently confirmed as a source of immune cells for immunotherapy such as dendritic cells (DCs) and cytokine-induced killer cells (CIKs). This study aimed to exploit this source of immune cells in banked UCB samples. After collection of UCB samples, mononuclear cells (MNCs) containing stem cells, progenitor cells, and mature cells were isolated by Ficoll-Hypaque-based centrifugation. The MNCs were subjected to freezing and thawing according to a previously published protocol. The banked MNCs were used to produce DCs and CIKs. To produce DCs, MNCs were induced in RPMI 1640 medium supplemented with GM-CSF (50 ng/ml) and IL-4 (40 ng/ml) for 14 days. To produce CIKs, MNCs were induced in RPMI 1640 medium supplemented an anti-CD3 monoclonal antibody, IL-3, and GMC-SF for 21–28 days. Both DCs and CIKs were evaluated for their phenotypes and functions according to previously published protocols. The results showed that banked UCB samples can be successfully used to produce functional DCs and CIKs. These samples are valuable sources of immune cells for immunotherapy. The present results suggest that banked UCB samples are useful not only for stem cell isolation, but also for immune cell production.
      PubDate: 2015-11-21
      Issue No: Vol. 2, No. 10 (2015)
  • Mini-invasive treatment for delayed or non-union: the use of percutaneous
           autologous bone marrow injection

    • Authors: Trung Hau LE THUA, Duy-Thang NGUYEN, Dang Nhat PHAM, Khanh-Linh LE, Quy Ngoc Bao LE, Phan Huy NGUYEN, Thi Thuy Hoa PHAN, Hoang Duy PHAN, Phuoc Quang LE, Willy Denis BOECKX, Albert DE MEY
      First page: 389
      Abstract: Delayed union or nonunion of bone fracture is becoming less frequent, but still remains a challenging clinical problem. Autologous cancellous bone grafting that is the gold standard method, often involves donor site morbidities and complications. Once these fractures have been mechanically stabilized, other local factors should be investigated to promote delayed healing. The purpose of this study was to evaluate the initial outcome of the percutaneous injection of autologous bone marrow after concentration for the treatment of delayed or nonunion. Our subjects included 10 patients of delayed or nonunion fracture (3 female, 7 male) with an average age of 28 years. All fractures were mechanically stabilized after accident. Delayed or nonunion affected the femur in 2 patients, the tibia in 5 patients, the humerus in 2 patients, and the ulna in 1 patient. Bone marrow aspirates were obtained from both the posterior superior iliac crest. Bone marrow aspiration concentrate was produced via density gradient centrifugation. Trocars were inserted in the delayed or nonunion gap under fluoroscopic guidance. The bone marrow aspiration concentrate was injected slowly. As results, all of 10 delayed or nonunion healed after treatment with percutaneous injection of autologous bone marrow. The mean time for new bone formation was 3.3 months, for clinical union was 5.2 months, and for radiological union was 11.8 months. The current study is encouraging in the initial outcome and percutaneous bone marrow implantation could be an effective and safe treatment for delayed or nonunion.
      PubDate: 2015-11-05
      Issue No: Vol. 2, No. 10 (2015)
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