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Journal Cover Biomedical Research and Therapy
  [1 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
   Published by Vietnam National University Homepage  [1 journal]
  • Is the neutrophil extracellular trap-driven microvascular inflammation
           essential for diabetes vasculopathy'

    • Authors: Alexander Berezin
      Pages: 618 - 624
      Abstract: The neutrophil extracellular traps (NETs) are defined as an extensive web consisting decondensed chromatin, which is released from activated neutrophils, as well as cytotoxic proteins, histones and microbicidal proteases that cause tissue damage. NETs contribute to endothelial damage, inflammation, thrombosis, platelet aggregation, ischemia, that are essential players in the pathobiology of diabetic complications. The objective of the review is to highlight the possible role of NETosis in early diabetes-related vasculopathy beyond cardiovascular complications. Although the clinical significance of NETosis in diabetes beyond early atherosclerosis and cardiovascular complications is not still clear, there is limited data with respect to useful to use biological markers of NETosis aimed early stratification of the diabetics at risk of disease progression. Furthermore, several inductors of NETosis might be a target for novel pharmacological approaches to delay advance in diabetes and prevent diabetes-related vasculopathy.
      PubDate: 2016-05-27
      Issue No: Vol. 3, No. 5 (2016)
  • Concise review: 3D cell culture systems for anticancer drug screening

    • Authors: Huyen Thi-Lam Nguyen, Sinh Truong Nguyen, Phuc Van Pham
      Pages: 625 - 632
      Abstract: Three-dimensional (3D) cultures are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than monolayer cultures. In cancer research, the natural tumor characteristics and architecture are more closely mimicked by 3D cell models. Thus, 3D cell cultures are more promising and suitable models, particularly for in vitro drug screening to predict in vivo efficacy. Different methods have been developed to create 3D cell culture systems for research application. This review will introduce and discuss 3D cell culture methods most popularly used in drug screening. The potential applications of these systems in anticancer drug screening will also be discussed.
      PubDate: 2016-05-27
      Issue No: Vol. 3, No. 5 (2016)
  • Effects of ricin extracted from seeds of the castor bean (ricinus
           communis) on cytotoxicity and tumorigenesis of melanoma cells

    • Authors: Trung Ngoc Ngo, Tho Thi Nguyen, Dung Thi-Thuy Bui, Nhung Thi-My Hoang, Thang Dinh Nguyen
      Pages: 633 - 644
      Abstract: Ricin has been reported as a potential therapeutic agent for the treatment of various cancers due to its potency. In this study, we succeeded in isolating and purifying total ricin from seeds of the castor bean (ricinus communis) from Vietnam. We also revealed that total ricin showed strong cytotoxicity against melanoma cells; IC50 at 48 h was 34.1 ng/mL for SKMEL28 cells and 5.2 ng/mL for HaCaT cells. We examined the ability of total ricin to inhibit tumorigenesis of SKMEL28 cells in vitro. At low concentrations (< 3ng/mL) total ricin did not cause death of HaCaT or SKMEL28 cells but strongly reduced the size of SKMEL28 tumor colonies formed in soft agar. The effect of ricin on tumorigenesis was also confirmed by apoptotic and immunoblot analyses. Our results showed that treatment with total ricin (3 ng/mL) resulted in decreased ERK and p-ERK expression in SKME28 cells but did not affect expression levels of those genes in HaCaT cells. We showed that, although ricin at 1 and 3 ng/mL did not induce apoptosis of HaCaT cells, it significantly increased apoptosis of SKMEL28 up to 1.4 folds and 2.1 folds, respectively. The results from our study suggest that although ricin is listed as one of the most poisonous substances in nature, it has potential to be used as a drug for melanoma treatment.
      PubDate: 2016-05-27
      Issue No: Vol. 3, No. 5 (2016)
  • Isolation of endothelial progenitor cells from human adipose tissue

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Hoa Trong Nguyen, Ngoc Kim Phan
      Pages: 645 - 652
      Abstract: Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.
      PubDate: 2016-05-27
      Issue No: Vol. 3, No. 5 (2016)
School of Mathematical and Computer Sciences
Heriot-Watt University
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