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Biomedical Research and Therapy
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  This is an Open Access Journal Open Access journal
     ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
     Published by Vietnam National University Homepage  [1 journal]
  • The Engimatic WNT Signaling and Mesenchymal Stem Cell Adipogenesis :
           Implications for Metabolic disorders

    • Authors: Nidhi Anil
      Abstract: The past decade has witnessed a sudden surge in the obesity prevalence worldwide. Obesity  has been linked to several chronic metabolic disorders including diabetes, hyperlipidemia and atherosclerosis. Due to this there is an immense interest in understanding the intricate aspects of adipogenesis, specifically pertaining to the study of the mechanisms through which various signaling pathways regulate adipocyte differentiation. One such enigmatic signaling  pathway  regulating adipogenesis is the WNT signaling Pathway. The present review focusses on the role of WNT signaling on Adipogenesis and its relationship with the development of metabolic disorders.
      PubDate: 2014-09-17
      Issue No: Vol. 1, No. 4 (2014)
  • Production of functional dendritic cells from mouse bone marrow

    • Authors: Viet Quoc Pham, Sinh Truong Nguyen, Phuc Van Pham
      Abstract: Immune cells based treatments, especially dendritic cells, become a promising therapy for cancer treatment nowadays. Therefore, dendritic cell therapy is focused to explore and develop the novel therapies in many laboratories in over the world. This study aimed to suggest a reproducible procedure to produce the functional dendritic cells from mouse bone marrow for dendritic cell therapy research. Bone marrow was collected from the mouse femur bones by flushing with buffer saline. These cells were used to isolate mononuclear cells (MNCs) by Ficoll gradient centrifugation. MNCs were culture in RPMI 1640 medium supplemented with IL-4 and GMCSF to induce to immature DCs. The results showed that by this procedure induced cells exhibited the DC phenotypes such as expression of CD40, CD80 and CD86, high phagotosis capacity, strongly production of IL-12, and efficiency stimulation of T-CD4 lymphocytes. By this result, this method can be applied to produce functional DCs for further studies about dendritic cells and immune therapy.
      PubDate: 2014-09-13
      Issue No: Vol. 1, No. 4 (2014)
  • Dendritic cells transfected with interleukin-12 and breast cancer stem
           cells derived mRNA induce high avidity and antigen specification of
           cytotoxic cells

    • Authors: Phuc Van Pham, Viet Quoc Pham, Sinh Truong Nguyen, Hanh Thi Le, Phong Minh Le, Ngu Van Trinh, Hoang Minh Nguyen, Nhan Lu-Chinh Phan, Ngoc Kim Phan
      Abstract: Breast cancer is the one of the most cancer in the women. For recent years, some novel therapies based monoclonal antibodies were significantly improved the treatment efficacy. However, some reports also showed that treatment efficacy of breast cancer with triple negative phenotypes or high breast cancer stem cells also too low by these therapies. Dendritic cells based immunotherapy is considered as a promising therapy for cancer treatment. This study aimed to improve the dendritic cells based treatment through enhancement of antigen presentation and stimulation of dendritic cells on cytotoxic cells. Immature dendritic cells were induced from umbilical cord blood derived mononuclear cells by RPMI 1640 medium supplemented with GMCSF and IL-4 at 30 ng/mL of each for 7 days. Interleukin-12 mRNAs and breast cancer stem cell mRNAs were in vitro synthesized by commercial kits. Immature dendritic cells were pulsed with mixture of IL-12 mRNAs and breast cancer stem cell mRNAs by electroporation. Mature DCs were tested the capacity of antigen presentation in vitro by real-time impedance based cell cytotoxicity assay. The results showed that dendritic cells transfected with both IL-12 mRNA and breast cancer stem cell mRNA significantly stimulated the cytotoxic cells, strongly inhibited the breast cancer stem cell proliferation in vitro in comparison to tumor lysates primed DCs. Different to tumor lysates primed DCs, DCs transfected with IL-12 and breast cancer stem cell mRNA efficiently both breast cancer cells and breast cancer stem cells derived from the same tumors. These results showed that DCs transfected with breast cancer stem cells, and IL-12 mRNA is a bright option to improve the treatment efficacy of breast cancer by dendritic cell-based therapy.
      PubDate: 2014-09-10
      Issue No: Vol. 1, No. 4 (2014)
  • Calretinin negative Paratesticular mesothelioma: a rare case report

    • Authors: Ankitha Hebbar, Kalyani Raju, Srinivas Murthy Venkataramappa
      Abstract: Malignant mesothelioma of the tunica vaginalis is rare. The etiology is unclear. Exposure to asbestos is attributed as one of the risk factor. It is commonly seen in the elderly age group with hydrocele as the presenting complaint. Diagnosis is usually confirmed following histopathological examination, marker studies by immunohistochemistry and electron microscopy. We present a case in a 54 year old male who presented with left scrotal swelling.  Immunohistochemically the tumor cells were positive for WT1, keratins, EMA, vimentin and negative for calretinin, desmin, CEA and actin. Ours is a rare case of paratesticular mesothelioma with calretinin negative.
      PubDate: 2014-08-26
      Issue No: Vol. 1, No. 4 (2014)
  • Lawsonia inermis (L.): A Perspective on Anticancer potential of

    • Authors: Dhananjay Kumar Singh, Suaib Luqman
      Abstract: Incidence of cancer is growing swiftly worldwide and sparse supply of anticancer drugs; unaffordable cost and its lethal effect have shown the way to adopt complementary and alternative medicine for the treatment and/or prevention of cancer.  The isolation of anti-cancer alkaloids vinblastine, vincristine and podophyllotoxins during 1950s prompts research on anticancer agents from plant origins. Lawsonia inermis (L.) popularly known as Mehndi or Henna, is a cosmetically renowned plant of the oriental region possesses diverse pharmacological activity including anti-carcinogenic, antimicrobial, anti-inflammatory, analgesic, antipyretic, hepatoprotective, anti-tuberculostatic. In search of new anticancer drugs from natural sources many researchers have reported anticancer and chemopreventive properties of Henna extracts/compounds in their pre-clinical studies. Lawsone, one of the major constituent of henna, is used as a starting material in the synthesis of a variety of clinically valuable anticancer drugs such as atovaquone, lapachol and dichloroallyl lawsone. This plant contain other chemicals such as isoplumbagin, apigenin, apigenin glycosides, luteolin, luteolin-7 glucosides, p-coumarin and lupeol among which many are reported for their cytotoxicity and chemopreventive activity against different type of cancer cell. Here in this review, we are reporting a palingenesis of information regarding anticancer potential of Mehndi/Henna. We have included maximum available information from its in vitro and in vivo studies by referring different websites, text books, notes, and articles, abstract, summary and consulting worldwide accepted scientific databases. The present review recapitulates some important findings on the anticancer potential of Mehndi/Henna and to a great extent more work has to be undertaken to explore its novel target (s). Future investigation on novel molecules from Mehndi/Henna may offer great hope for discovering new cancer chemotherapeutic and/or chemopreventive agents from this miraculous plant.
      PubDate: 2014-08-26
      Issue No: Vol. 1, No. 4 (2014)
  • Good manufacturing practice-compliant isolation and culture of human
           adipose derived stem cells

    • Authors: Phuc Van Pham, Ngoc Bich Vu, Nhan Lu-Chinh Phan, Dung Minh Le, Nhat Chau Truong, Nhung Hai Truong, Khanh Hong-Thien Bui, Ngoc Kim Phan
      Abstract: Adipose-derived stem cells (ADSCs) are wonderful cells for regenerative medicine. Like mesenchymal stem cells, ADSCs hold multi-potent differentiation capacity that can differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into some other cells. ADSC transplantation rapidly increased for recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 10th passage could not form tumors in NOD/SCID mice. This research contributed a suitable protocol for clinical applications of expanded ADSCs.
      PubDate: 2014-07-05
      Issue No: Vol. 1, No. 3 (2014)
  • Direct reprogramming of somatic cells: an update

    • Authors: Phuc Van Pham
      Abstract:             Direct reprogramming is a technique that converting an adult cell into another differentiated cells – such from fibroblasts into cardiomyocytes – without passage through an undifferentiated pluripotent stage. This is a novel technology that opening a new chance for both biological research and regenerative medicine. Some preliminary studies about direct reprogramming started in the 1980s when differentiated adult cells could be converted into another differentiated cells by over-expressing some transcription factor genes. These studies showed that differentiated cells or mature cells also have the plasticity. To date, direct reprogramming becomes a powerful tool in the biological research and regenerative medicine, especially personalized medicine. Here, this review aimed to summarize all direct reprogramming studies of somatic cells by master control genes into others as well as potential applications of these techniques in research and treatment of some human diseases.
      PubDate: 2014-06-28
      Issue No: Vol. 1, No. 3 (2014)
  • Oncolytic virotherapy for cancer

    • Authors: Kotovoe Erlomeava
      Abstract: Cancer is the leading caused of death in human. In recent years, there are some therapies developed in the diagnosis and treatment of cancers. However, overall outcome is not efficiently improved. Virotherapy using oncolytic viruses is considered as novel strategy for cancer treatment. In principle, virotherapy used oncolytic viruses that can infect and lyse target cancer cells. Because oncolytic viruses can specific infect to target cells through receptors in cell surface. So this therapy promises without damage with healthy tissue. This review aimed to describe the use of oncolytic viruses in cancer treatment in recent years.
      PubDate: 2014-06-13
      Issue No: Vol. 1, No. 3 (2014)
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