Biomedical Research and Therapy
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Open Access journal
ISSN (Print) 2198-4093 - ISSN (Online) 2198-4093
Published by Vietnam National University [1 journal]
- Virus and Cervical Cancer: Role and implication: A Review
Authors: Kalyani Raju
Abstract: Cervical cancer is one of the leading cancers in women worldwide especially in developing countries. Various etiological factors are described, of which Human papiloma virus (HPV) is proved by various molecular epidemiological studies to play a major role. However many co-factors are required and thought to facilitate the action of HPV in cervical carcinogenesis. Here the role of various viruses in cervical cancer and its implication in screening and diagnosis of cervical cancer is highlighted. In-depth knowledge of role of different viruses helps in better screening methods and probably in target therapy / development of an appropriate vaccine.
Issue No: Vol. 2, No. 2 (2015)
- Retinoic acid improves the cisplatin effect on tumor initiating cells in
Authors: Julie Arnold, William Hwang, Sudipto Bari, Hong Zhang
Abstract: In recent years, cisplatin is used in hepatocellular carcinoma (HCC) treatment as adjuvant treatment for post-operation patients. However, its efficiency also was largely limited because of the high incidence of chemoresistance. It was the aim of this study to assess the effects of cisplatin in combination with retinoic acid (RA) on differentiation and apoptosis of tumor-initiating cells (TICs) in human HCC. In this study, TICs would be treated cisplatin+RA and cisplatin or RA alone as the controls. The TIC differentiation was evaluated by phenotype changes while TIC apoptosis was evaluated by PI-annexin V assay. The results showed that RA effectively induced differentiation of TICs, which potentiated the cytotoxic effects of cisplatin. The combinatorial treatment of RA and cisplatin significantly promoted apoptosis, and differentiation of human TICs compared to the treatment with either drug alone. These findings demonstrated that the combination of RA with cisplatin may be a promising strategy in HCC treatment.
Issue No: Vol. 2, No. 1 (2015)
- In vitro spontaneous differentiation of human breast cancer stem cell and
Authors: Phuc Van Pham
Abstract: Breast cancer stem cells were considered as origins of breast cancer. Previously published studies showed that breast cancer stem cells exhibited high multi-drug resistance. This study aimed to evaluate the spontaneous differentiation of human breast cancer stem cells and investigate some in vitro conditions to control this process. Human breast cancer stem cells (BCSCs) were sorted from primary culture of breast malignant tumors based on expression of CD44 and CD24. The in vitro spontaneous differentiation of BCSCs was evaluated in the popular culture medium DMEM/F12 supplemented with 10% fetal bovine serum (FBS), 1% antibiotic-antimycotic. There were some different methods to control the spontaneous differentiation of BCSCs included free serum culture, mammosphere culture, basic fibroblast growth factor and epidermal growth factor supplement to serum medium, and hypoxia culture. The results showed that BCSCs always were spontaneously differentiated in vitro in the popular culture medium DMEM/F12 plus 10% FBS. The percentage of BCSCs gradually decreased according to sub-culture times and became stable after 20 sub-culture times. All investigated methods could not completely inhibit the spontaneous differentiation of BCSCs. Serum-free culture combined with hypoxia condition had strongest inhibition of this process. These results demonstrated that the spontaneous differentiation is nature process of BCSCs; therefore this process should be determined and suitably controlled depending on different experiments.
Issue No: Vol. 2, No. 1 (2015)
- Evaluation in vitro senescence of mesenchymal stem cells isolated from
mouse bone marrow and adipose tissue
Authors: Sinai Walker, Gene Sam, Martin Hill, Matthie Robert
Abstract: Mesenchymal stem cells (MSCs) from bone marrow and adipose tissue are intensively used for regenerative medicine. This study aimed to compare the in vitro senescence of MSCs isolated from adipose (BM-MSCs) and bone marrow tissues (ADSCs). Both BM-MSCs and ADSCs were isolated from the same mice, and sub-cultured to 3rd passage. These MSCs were assessed cell proliferation, senescence associated beta-galactosidase staining, and telomere length as well as stemness marker expression. The results showed that both BM-MSCs and ADSCs would go to senescence after long-term culture. BM-MSCs significantly decreased the proliferation rate after passage 20, while ADSCs could strongly proliferate to passage 50. Based on beta-galactosidase expression, BM-MSCs also were positive sooner than ADSCs (passage 10 vs. passage 20, respectively). At these passages, both BM-MSCs and ADSCs exhibited the shortening of telomere length from 10.4 to 5.2 kbps in passage 3 to senescent cultures, respectively. In conclusion, MSCs from bone marrow appear to senesce much earlier than those from adipose tissue. These results suggested that numbers of sub-culture should be controlled to get MSCs in good status for research or application.
Issue No: Vol. 2, No. 1 (2015)
- Characterization and expression of pluripotency markers in human dental
pulp stem cells
Authors: Miglino Maria, Laurino Carlos, Rosalia Mendez-Otero, Natasha Machado
Abstract: Human dental pulp stem cell (DPSC) is new source of stem cells. In this study, we characterized expression of pluripotency markers such as Oct-3/4, Sox-2, and Nanog in DPSCs. DPSCs were isolated according to the published procedure. They were sub-cultured to 3rd passage and used for further experiments. Firstly, DPSCs were characterized with mesenchymal stem cell phenotypes included expression of CD29, CD44, CD73, CD90 and CD105; adipogenesis and osteogenesis differentiation. Secondly, DPSCs were checked expression of Oct-3/4, Sox-2, and Nanog by both RT-PCR, immunofluorescence. The results showed that DPSCs satisfied the minimal criteria of MSCs. Moreover, they also expressed the pluripotent markers Oct-3/4, Sox-2, and Nanog at both mRNA and protein level. Thus, these factors may have a potential role in influencing the high proliferation and differentiation of DPSCs. In conclusion, this result showed that DPSCs represent as autologous stem cell source with high potential for dental engineering as well as regenerative medicine.
Issue No: Vol. 2, No. 1 (2015)
- Oncolytic virotherapy for cancer
Authors: Kotovoe Erlomeava
Abstract: Cancer is the leading caused of death in human. In recent years, there are some therapies developed in the diagnosis and treatment of cancers. However, overall outcome is not efficiently improved. Virotherapy using oncolytic viruses is considered as novel strategy for cancer treatment. In principle, virotherapy used oncolytic viruses that can infect and lyse target cancer cells. Because oncolytic viruses can specific infect to target cells through receptors in cell surface. So this therapy promises without damage with healthy tissue. This review aimed to describe the use of oncolytic viruses in cancer treatment in recent years.
Issue No: Vol. 2, No. 1 (2014)
- Hypoxia condition promoted the adipose derived stem cell proliferation via
Authors: Phuc Van Pham, Ngoc Bich Vu, Nhung Hai Truong, Loan Thi-Tung Dang, Nhan Lu-Chinh Phan, Ngoc Kim Phan
Abstract: Adipose-derived stem cells (ADSCs) are a promising mesenchymal stem cells source with therapeutic applications. Some recent studies showed that ADSCs could be expanded in vitro without phenotype changes. This study aimed to evaluate the effect of hypoxia condition on ADSC proliferation in vitro and to determine the role of VEGF in ADSC proliferation. ADSCs were selectively cultured from stromal vascular fraction that obtained from adipose tissue in the DMEM/F12 medium supplemented with 10% FBS, 1% antibiotic-antimycotic. ADSCs were cultured in two conditions included hypoxia (5% O2) and normal oxygen (21% O2). Effects of oxygen concentration on cell proliferation were recorded by cell cycle and doubling time. Expression of VEGF was evaluated by real-time RT-PCR and ELISA assays. And the role of VEGF on ADSC proliferation was studied by neutralizing VEGF with anti-VEGF monoclonal antibody. The results showed that ADSC proliferation rate was increased 2.5 times in hypoxia compared to normal oxygen. And hypoxia condition, ADSCs also triggered VEGF synthesis at both mRNA and translational level. However, neutralizing VEGF with anti-VEGF monoclonal antibody significantly reduced the proliferation rate. These results suggested that hypoxia stimulated ADSC proliferation related to VEGF production. This finding will contribute not only in stem cell technology but also in obese treatment.
Issue No: Vol. 1, No. 5 (2014)
- Direct reprogramming of somatic cells: an update
Authors: Phuc Van Pham
Abstract: Direct reprogramming is a technique that converting an adult cell into another differentiated cells – such from fibroblasts into cardiomyocytes – without passage through an undifferentiated pluripotent stage. This is a novel technology that opening a new chance for both biological research and regenerative medicine. Some preliminary studies about direct reprogramming started in the 1980s when differentiated adult cells could be converted into another differentiated cells by over-expressing some transcription factor genes. These studies showed that differentiated cells or mature cells also have the plasticity. To date, direct reprogramming becomes a powerful tool in the biological research and regenerative medicine, especially personalized medicine. Here, this review aimed to summarize all direct reprogramming studies of somatic cells by master control genes into others as well as potential applications of these techniques in research and treatment of some human diseases.
Issue No: Vol. 1, No. 3 (2014)
- Changes blood protein composition under experimental chemical burns of
esophageal development in rats
Authors: Tetiana Ishchuk, Yana Raetska, Olexiy Savchuk, Ludmila Ostapchenko
Abstract: Exogenous poisoning with alkalis takes the leading position among causes of acute poisoning. Esophageal burns as a result of accidental swallowing of caustic material are seen frequently in children ages 1 to 8 years. A burn wound is perhaps the most intense stress that a human body can suffer. In the place of chemical trauma localization the processes of synthesis and degradation of proteins increase. As a result the structure and functions of vital organs and immune system suffer. The inflammation process after burn injury is determines the changes in protein expression. In our research we have shown that chemical burn of the esophagus is characterized by decreased level of total proteolytic activity, total protein and development of endogenous intoxication of the body as indicated by elevated MMM level. Obtained results suggest that the development of chemical burns of the esophagus grade 2 is characterized by more substantial changes in the protein composition of blood compared with grade 1. An in-depth study of this disease has a crucial scientific and practical importance for development of pathogenically oriented methods of wound process healing after the chemical esophageal burns depending on the stage and extent of the burn process
Issue No: Vol. 2, No. 3