International Journal of Biotechnology and Molecular Biology Research
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Open Access journal
ISSN (Print) 2141-2154
Published by Academic Journals [72 journals]
- Characterization of gamma-irradiated seeds of a wild Namibian marama bean
(Tylosema esculentum) with microsatellite markers
Abstract: Legumes have been used as food by indigenous people of Africa yet their full potential as food sources has not been completely realized. With modern technologies such as marker-assisted selection and mutational breeding, there is scope for rapid improvement of germplasm in these underutilized species. One approach is to induce genetic mutations through irradiation of plant tissues with neutr...
PubDate: Thu, 31 Dec 2015 00:00:00 +010
- In vitro propagation of two elite cooking banana cultivars- FHIA 17 and
Abstract: Explants of two elite cooking banana cultivars, FHIA17 and INJAGI were collected from healthy source of stock plants growing in the field. Sterilization was evaluated using different concentrations (20, 30, 40, 50 and 60%) of a commercial bleach (JIK) for 25 min. Effects of cytokinins benzyl amino purine (BAP), 2-isopentenyl adenine (2iP) and kinetin each evaluated at 5, 10, 20, 24 and 40 M ...
PubDate: Mon, 30 Nov 2015 00:00:00 +010
- RNA Interference – A fine tuner of gene regulation: a Review
Abstract: The discovery of RNA interference by Fire and Mello has opened up a new arena for research in molecular biology. The interfering RNAs (iRNAs) are 21-23 nucleotide RNA duplexes, which have the ability to reduce or abolish gene activity, and this property is being used in reverse genetics. Dicer and RISC (RNA-induced silencing complex) play important role in mechanism of RNA interference. The ...
PubDate: Tue, 30 Jun 2015 00:00:00 +010
- Induction and regeneration of somatic embryos from Vitex doniana
(Lamiaceae) leaf explants
Abstract: The present study was conducted with the aim of evaluating some of the factors that influence induction and regeneration of somatic embryos in Vitex doniana since there are no available reports on tissue culture of this tree species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog supplemented with silver nitrate and four amino acids (proline, tryptophan,...
PubDate: Sun, 31 May 2015 00:00:00 +010
- Production, purification and characterization of a thermostable alkaline
serine protease from Bacillus lichniformis NMS-1
Abstract: Alkaline proteases are used in food industry, leather tanning and processing industry, preparation of pharmaceuticals and also in the fiber industry. An alkaline serine protease producing strain was isolated using soil sample from a natural hot water spring in Sri Lanka. It was identified based on morphological, biochemical and 16s rRNA identifications as Bacillus licheniformis NMS-1. The ex...
PubDate: Tue, 31 Mar 2015 00:00:00 +010
- Genetic diversity of sesame germplasm collection (SESAMUM INDICUM L.):
implication for conservation, improvement and use
Abstract: Genetic diversity assessment of genetic resources maintained at Gene-Banks has important implication for future improvement, conservation and collection activities. However, such information is not available for sesame collected by IBC, Ethiopia. Inter simple sequence repeat (ISSR) marker was used to assess the level of genetic diversity, genetic structure and genetic distance, and to indire...
PubDate: Sat, 28 Feb 2015 00:00:00 +010
- A new method for the detection of oil degrading genes in Pseudomonas
aeruginosa based on transformation and PCR hybridization
Abstract: Biodegradation is the chemical breakdown of materials by a physiological environment. The term is often used in relation to ecology, waste management and environmental remediation. A specific gene from bacteria is identified and sequenced which has the capacity of oil degradation without any obvious strain specific discrimination using a combination of PCR and hybridization. The parameters o...
PubDate: Sat, 31 Jan 2015 00:00:00 +010
- Molecular characterization of resistance to Russian wheat aphid (Diuraphis
noxia Kurdjumov) in bread wheat (Triticum aestivum L.) line KRWA9
Abstract: The Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov) causes extensive economic damage to wheat (Triticum aestivum L.) in most wheat growing regions of the world. Control of RWA using systemic insecticides is expensive and pollutes the environment therefore the most effective method of RWA control is the development of RWA resistant cultivars. This study was initiated to determine inher...
PubDate: Wed, 31 Dec 2014 00:00:00 +010
- Molecular identification of Lactobacillus plantarum isolated from
Abstract: The identification of a microbial isolate to genus level only amounts to a partial characterization of the isolate, but this can tell us a lot about that organism. Knowing the species allows the laboratory access to the body of knowledge that exists on that species. Identification schemes using phenotypic characteristics such as colony and cell morphology, Gram reaction and other staining ch...
PubDate: Wed, 31 Dec 2014 00:00:00 +010
- Assessment of different samples for molecular diagnosis of extra-pulmonary
Abstract: Extrapulmonary tuberculosis is an important clinical problem particularly in developing countries. The aim of this study was to assess two different samples (blood and fluid) for the diagnosis of extra-pulmonary tuberculosis (abdominal tuberculosis and tuberculous lymphadenitis). The study subjects were recruited from WadMedani Teaching Hospital during 2009-2013. Seventy five ascetic fluid a...
PubDate: Sun, 31 Aug 2014 00:00:00 +010