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Journal Cover Journal of Food and Pharmaceutical Sciences
  [1 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2089-7200 - ISSN (Online) 2339-0948
   Published by Gadjah Mada University Homepage  [4 journals]
  • ELECTRICAL STUNNING AND PRE-SLAUGHTERING TIME INTERVAL EFFECT IN
           EXPRESSION OF HEART BROILER CHICKENS PROTEIN

    • Authors: Sandra Hermanto
      Abstract: Pre-slaughtering process by transporting broiler chickens from farm to slaughterhouse and treatment of electrical stunning could be one of halal critical point. Stressing on chickens could be trigger a specific protein expression or increase its expression as a result of changes in metabolism that occurs. The purpose of this study was to identify the effect of treatment of electrical stunning and the pre-slaughtering interval time on broiler chicken liver protein expression through the analysis of protein profile by SDS-PAGE. Experiments conducted on 7 broiler chickens aged 35 days were given pre-slaughter treatment are different. Treatments include electrical stunning of 0 V, 25 V and 250 V as well as the pre-slaughtering time interval 0 hours, 2 hours, 2 hours + 12 hours (fasting) and 2 hours + 12 (fasting) with electrical stunning 250 V. Each of samples (chicken liver protein) isolated by homogenized in Trs-HCl buffer and the protein concentration determined by Lowry method. Identification of the protein profile was done by observing the differences in the intensity of the electrophoresis results followed by densitometry analysis. The results showed significant differences in outcomes electrical stunning treatment as indicated by the appearance of protein bands at around 109 kDa and do not appear on other treatments. The difference is predicted from particular protein expressed induced by cell metabolism responsive in chicken liver cell.
      PubDate: 2016-03-29
      Issue No: Vol. 3, No. 3 (2016)
       
  • Tiron Mitigates Thioacetamide-Induced Acute Liver Injury

    • Authors: Amal Mahmoud Shoeib, Eman Said, Manar Ahmed Nader, Hatem Abd-Elrahman Salem, Elsayed Mohamed Ammar
      Abstract: Acute liver injury is a crippling disorder accompanied by extensive impairment of liver’s synthetic, metabolic and detoxifying functions. Tiron is a synthetic vitamin E analog, proven to be anti-oxidant. This study was undertaken to investigate the protective activity of tiron against thioacetamide (TAA)-induced acute liver injury. Rats were orally treated with tiron (300 mg/kg) for eight days prior to I.V. TAA either (70 mg/kg) or (280 mg/kg) to induce acute liver injury. Biochemical evaluation of hepatotoxicity indices, oxidative stress, cytotoxicity and inflammatory marker: interleukin-6 (IL-6) was conducted along with histopathological examination. Meanwhile, tiron was found to mitigate the TAA-induced elevation of ALT, AST and ALP. However, serum albumin levels mildly improved. Tiron restored liver GSH contents and maintained liver SOD activity. Moreover, tiron significantly reduced the level of serum IL-6. In context, histopathological examination revealed that tiron slightly reduced the extent of TAA-induced necrosis. Tiron has manifested the observed hepatoprotective effect probably by manipulating inflammatory response of liver to injury via downregulating the expression of inflammatory IL-6 and alleviating oxidative stress.  
      PubDate: 2016-03-29
      Issue No: Vol. 3, No. 3 (2016)
       
  • DETERMINATION OF NUTRIENT COMPOSITIONS, GLYCEAMIC INDEX AND ANT-DIABETIC
           POTENTIALS OF MULTI-PLANT BASED FUNCTIONAL FOODS IN RATS

    • Authors: Ijarotimi Oluwole Steve
      Abstract: Background: Nutrition transition, that is, increase in consumption of high energy-dense foods, with low consumption of fruits and vegetables, has been implicated as the major factors responsible for the increase in prevalence of diet-related diseases such as diabetes and cardiovascular diseases worldwide. Evidence has shown that consumption of plant-based foods prevents the risk of these chronic diseases, hence, the present study formulated and evaluated nutrient compositions and antidiabetic potentials of multi-plant based functional foods from locally available food materials.Methods: Food materials (popcorn, moringa leaves, wonderful kola and defatted soybean) were obtained from reputable farms and markets in Akure, Nigeria. The food materials were processed as raw, blanched and fermented flour samples, and blended to obtain nine samples, i.e., R1, B1 and F1 (popcorn 60%, soybean 10%, moringa 20% and groundnut oil 10%), R2, B2 and F2 (popcorn 60%, soybean 10%, wonderful kola 20% and groundnut oil 10%) and R3, B3 and F3 (popcorn 60%, soybean 10%, moringa 10%, wonderful kola 10% and groundnut oil 10%) using  Nutri-Survey software. Proximate compositions of the blended samples were determined using AOAC methods, and glyceamic index and anti-diabetic potentials were determined using rat models.Results: Proximate compositions (g/100g) of the formulated multi-plant based functional foods were as follows: moisture contents ranged from 6.29 in F3 to 8.27 in R3, crude fiber contents from 2.79 in F2 to 4.68 in B3 and crude protein contents from 23.22 in B1 to 30.39 in F3, while carbohydrate content of the formulations ranged from 52.10 in F3 sample to 56.94 in B2 sample, while energy values were between 421.1 in R1 and 433.7 kcal in B1. Glycaemic index (GI) of the formulated functional foods ranged from 5.8% in R3 to 28.9% in R1 sample, and were significantly (<0.05) lower than in glucose (a reference sample). The glycaemic load (GL) of R4 sample had the lowest value (3.3), while F4 sample had the highest value (15.1). The percentage blood glucose reduction of diabetic-induced rats fed with R1samples (63.8%) had the highest values; while those rats fed with B2 sample (24.1%) had the lowest blood glucose reduction. Statistically, the percentage blood glucose reduction of the formulated functional foods, particularly R1 and F1, were comparable to metformin (a synthetic anti-diabetic drug) in terms of antidiabetic activities.  Conclusion: The study reported on the nutritional profile of multi-plant based functional foods from popcorn, soybean, wonderful kola and moringa leaves. Findings showed that these functional foods contain appreciable amount of protein, fiber, carbohydrate content within the recommended value for diabetic patients, low glycaemic index and glycaemic load properties and with antidiabetic activities. Hence, the formulated functional foods may be suitable for individuals at risk of diabetes or diabetic patients.
      PubDate: 2016-03-29
      Issue No: Vol. 3, No. 3 (2016)
       
  • Antimicrobial and Volatile Compounds Study of Four Spices Commonly Used in
           Indonesian Culinary

    • Authors: Alwani Hamad, M. Gigih Panji Mahardika, Istifah Istifah, Dwi Hartanti
      Abstract: The n-hexane extracts of the aerial parts of Ocimum x citriodorum and the leaves of Cymbopogon citratus, Syzygium aromaticum and Syzygium polyanthum were evaluated for their antimicrobial activity against some food borne microorganisms. Their volatile compounds were analyzed by gas chromatography/mass spectrometry (GC/MS). All extracts inhibited the growth of Bacillus subtilis. The extract of S. polyanthum showed the strongest inhibitory activity against Salmonella typhimurium. The growth of Staphylococcus aureus, Escherichia coli and Vibrio cholera were not inhibited by all extracts. The major volatile constituent of O. x citriodorum, C. citratus, S. aromaticum and S. polyanthum crude extracts were citral, germacrene D-14-ol, p-eugenol and squalene, respectively.
      PubDate: 2016-03-29
      Issue No: Vol. 3, No. 3 (2016)
       
 
 
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