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Journal of Food and Pharmaceutical Sciences
  This is an Open Access Journal Open Access journal
     ISSN (Print) 2089-7200 - ISSN (Online) 2339-0948
     Published by Gadjah Mada University Homepage  [3 journals]
  • Protein Profilesof Beef (Bos indicus), Pork (Sus domesticus),and
           SausagesBy Using SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel
           Electrophoresis) Method

    • Authors: Zilhadia Zilhadia
      Abstract: A research has been done to analyze the protein profile in fresh beef, fresh pork, and 10 beef sausage by using SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) with 2 plate gel electrophoresis. From this research, we found several protein bands that become distinctive protein bands. On raw beef protein we found three bands that are not found in pork. They are protein band with molecular weight (MW) of 144,54 kDa, 81,28 kDa and 58,88 kDa respectively. On the raw pork, we found 5 protein bands that are not found in raw beef, namely protein bands with MW 154,88 kDa; 146,55 kDa; 83,18 kDa; 69,18 kDa and 61,66 kDa. There is a band on pork protein found on the second plate on MW gel is 69,18 kDa. Whereas in 10 samples of beef sausages we did not found any specific protein bands. This is presumably due to the difference in manufacturing process performed by the manufacturer.
      PubDate: 2013-10-15
      Issue No: Vol. 2, No. 2 (2013)
  • Antioxidant Activity of Stevia(Stevia rebaudianaL.) Leaf Powder and A
           Commercial Stevioside Powder

    • Authors: Galla Narsing Rao
      Abstract: Stevia leaf powder (SLP) and a commercial stevioside powder (CSP) were analysed for their polyphenol content, isothermal sorption behavior and their antioxidant activity was evaluated by DPPH radical scavenging activity, ferric reducing power and ABTS assay. Polyphenol contents were higher (5.6%) in SLP than in CSP (2.3%). It was observed that the SLP was non-hygroscopic and CSP was hygroscopic in nature as seen from sorption isotherms. The equilibrium relative humidity of SLP and CSP respectively was 62 and 24%. The antioxidant activity of the methanolic extracts were determined and DPPH radical scavenging activity for SLP showed 30.33% for 20 µg/ml and 52.46% inhibition at 100 µg/ml, which were slightly higher compared to CSP stevioside, which showed 29.5% and 47.64% inhibition for similar concentrations.Higher ferric reducing power was seen in CSP (0.632) than SLP (0.166) for 100 µg.The antioxidant activity measured by ABTS assay also indicated higher activity (37.5% inhibition for10 µg/ml) for SLP than CSP (39.66% for 40 µg/ml).
      PubDate: 2013-07-30
      Issue No: Vol. 2, No. 2 (2013)
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