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Journal Cover Journal of Plant Breeding and Genetics
  [10 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2308-121X - ISSN (Online) 2305-297X
   Published by eSci Journals Publishing Homepage  [8 journals]
  • DETERMINATION OF THE GENETIC INHERITANCE OF THE OVULE FUZZLESS TRAIT IN
           GOSSYPIUM ARBOREUM GERMPLASM

    • Authors: John E. Erpelding
      Pages: 01 - 05
      Abstract: The United States Department of Agriculture, National Plant Germplasm System Gossypium arboreum collection is an important source of genetic diversity for cotton improvement. Inheritance of the fuzzless trait was determined by crossing PI 615737 x PI 529729 (ovule fuzzy). Seeds from F1 plants showed ovule fuzz development and the F2 segregation data fit a single recessive gene model for the fuzzless trait. Additionally, results indicate incomplete dominance for ovule fuzz development. The information generated from the study will aid in further determination of the ovule fuzzless trait inheritance in cotton.
      PubDate: 2016-07-20
      Issue No: Vol. 1, No. 1 (2016)
       
  • INDUCTION OF MALE STERILITY IN SESAME (SESAMUM INDICUM L.)

    • Authors: Kalyan D. Savant
      Pages: 07 - 10
      Abstract: Hybridization and mutagenesis have been applied in India since the 1970’s in order to improve several seed crops as wheat, soybean, maize, groundnut, sunflower, rice, sesame, cowpea, moth bean, etc. Many mutant varieties were approved as national varieties and some promising regional lines through mutagenesis. Though exhaustive work has not been done on the male sterility systems and identification of restorers in Sesamum, there are few studies on genetic male sterility (GMS) and cytoplasmic male sterility (CMS) and also reports of male sterility during the study of cytogenetics or crossability using wild species in sesame. The possible applications of genetic male sterility (GMS) in plant breeding are reviewed and discussed. The basic contribution of GMS is that it provides a means of genetic emasculation which can be applied for the massive production of hybrids. There are two main fields of application, the production of hybrid varieties and inter- and intraspecific hybridization and back-crossing programmes for the introduction of genetic variation into crop varieties. Induction of genetic male sterility system coupled with natural honey bee activity can provide an effective tool for hybrid seed production in sesame. An attempt was made to induce genic male sterility system through a chemical mutagenesis. Two male sterile plants have been developed and maintained through sib mating. The male sterility system was found to be unstable in sesame. Stable male sterile lines are to be selected through repeated selection.
      PubDate: 2016-07-20
      Issue No: Vol. 1, No. 1 (2016)
       
  • OPTIMIZING GENOMIC DNA ISOLATION IN PINEAPPLE (ANANAS COMOSUS L.)

    • Pages: 11 - 18
      Abstract: The isolation of high molecular mass genomic DNA (Deoxyribonucleic acid) is crucial for applications in molecular biology. To this end many protocols were developed for the extraction of plant DNA. However, for pineapple (Ananas comosus), standard protocols are scarce and not always efficient when resources are limited. In this study, we developed a new protocol for nuclear DNA extraction in pineapple. Four existing protocols were tested but none has provided high quality DNA extract. The original laboratory standard protocol based on the use of CTAB (Cetyltrimethylammonium bromide) was successfully modified to optimize the quality of the DNA extract using eighteen pineapple young leaf samples including three parts: the leaf apex, the mid blade, and the leaf base of two cultivars (i.e. Sugarloaf and Smooth Cayenne). The successful extraction of DNA in a sister species, Ananas bracteatus, gave evidence that the protocol can be used for others Bromeliaceae. The new protocol yielded 51.76 µg/ml DNA, which is higher than that obtained with previous protocols. The DNA extract was efficiently PCR (Polymerase Chain Reaction) amplified using simple sequence repeat primers. We proposed henceforth the use of this protocol for further DNA isolation in pineapple particularly under limited resources condition when using CTAB.
      PubDate: 2016-07-20
      Issue No: Vol. 1, No. 1 (2016)
       
 
 
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