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  Journal of Plant Breeding and Genetics
  [10 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2308-121X - ISSN (Online) 2305-297X
   Published by eSci Journals Publishing Homepage  [8 journals]
  • STUDIES ON GENETIC DIVERGENCE IN GOSSYPIUM ARBOREUM L.

    • Authors: Rumesh Ranjan, Rajvir S. Sangwan, Surender S. Siwach, Omender Sangwan, Mitali Sah, Nancee Singh
      Pages: 57 - 61
      Abstract: On the basis of D2 values, sixty genotypes were grouped into eight clusters containing one to fourteen genotypes. These clusters consisted of genotypes with different geographical origins and indicated no correlation between genetic divergence and geographical divergence. The genotypes of Cluster VIII showed maximum genetic divergence with Cluster I and cluster V. The genotypes belonging to cluster VIII and cluster I may be selected for hybridization for generating genetic variability. Cluster VI having six genotypes was found to be best performing for agronomic characters followed by cluster VIII with one genotype and cluster V with eight genotypes. Thus to generate desirable genetic variability the crossing between cluster VI, VIII and V genotypes would be useful. It is suggested that hybridization among the genotypes of above said clusters would produce segregants for more than one economic character which can serve as parents of hybrids. Days to first flower followed by seed cotton yield per plant, number of monopods and plant height contribute maximum toward divergence.
      PubDate: 2014-08-14
      Issue No: Vol. 2, No. 2 (2014)
       
  • STUDYING GXE INTERACTION UNDER DIFFERENT MANAGEMENT SYSTEM AND YIELD LEVEL
           USING LINEAR-BILINEAR MODELS: THE CASE OF CIMMYT INTERMEDIATE TO LATE
           HYBRIDS TRAILS IN EASTERN AFRICA

    • Authors: Girma Taye, Dan Makumbi
      Pages: 63 - 76
      Abstract: CIMMYT, with national programs, conducts selection of stress-tolerant genotypes under managed stress conditions; this investigation is expected to add information to the existing knowledge. Data sets used in this study comes from Intermediate to Late Hybrid Trails (ILHT) conducted in five Eastern and Central Africa (ECA) countries from 2008 to 2011. Trials, ranging from 18 in 2009 to 29 in 2010 were used. Trials are categorized into four management systems and two yield levels. Variance Components, broad sense heritability (H), Site Regression (SREG), Genotypic Regression (GREG), Completely Multiplicative Model (COMM) and Factor Analytic (FA) models were fitted. Results are discussed and compared with those stated in literature. We argue that it is preferable to first fit the fixed effect models before proceeding to the mixed effect model, as the former shows the level of complexity of the GE component and number of Axis required to explain it. The fixed effect model, SREG2, is preferable for trails targeting to compare hybrids with checks. From the GGE biplots it was noted that the first two PC did not account for sufficient percentage of variation for all years which witnessed complexity in the GE component for this data. Nevertheless, since PC1 accounted for large percentage of variation than PC2, the plot still gives some idea of which hybrids are favored and where. Most importantly, location of genotypes along PC1 can serve for judging yielding potential of the genotypes to guide in selection decision. Equivalence between Finlay – Wilkinson and GREG was established. The few environmental covariables obtained for 2009 was used to fit Partial Least Square (PLS) regression. The result indicated complexity in the GE component, as PLS latent factors accounted for small percentage of variation. It was recommended to use information from SREG2, GREG2 and FA(1) models in order to identify stable genotype.
      PubDate: 2014-08-14
      Issue No: Vol. 2, No. 2 (2014)
       
  • ASSESSMENT OF NIGERIAN WILD OIL PALM (ELAEIS GUINEENSIS JACQ.) POPULATIONS
           IN CROSSES WITH DELI TESTERS

    • Authors: Noumouha E. N. Ghislain, Allou Désiré, Adon Benjamin, Konan Jean-Noël, Sékou Diabaté, Konan K. Eugène, Simon-Pierre A. Nguetta
      Pages: 77 - 86
      Abstract: To widen the genetic variability and especially to enrich agronomic qualities of populations of oil palm (Elaeis guineensis Jacq.) of Group B used in the breeding scheme, a study was led on four populations (Abak, Ahoada, Ayangba and Uli) prospected in Nigeria. Sixteen palms chosen in these populations were evaluated in crosses with 10 Deli testers derived from the second cycle of reciprocal recurrent selection scheme. The LM 2 T × DA 10 D and LM 2 T × DA 115 D progenies derived from the first breeding cycle were used as controls. The bunch production components, vascular wilt susceptibility and vertical growth rate were used as criterion of evaluation. The Abak × Deli, Ahoada × Deli and Uli × Deli materials were characterized at the young age by bunch productions higher than that of the LM 2 T × DA 10 D control. The bunch productions at the adult period of various Nigerian × Deli materials were lower than those of the two controls. Abak × Deli material was tolerant to the vascular wilt. Ayangba × Deli material had a slower vertical growth rate than those of the two controls. Seven parents were identified in the four populations for their good general combining ability for the precocity and/or vascular wilt tolerance or the slow vertical growth rate. Prospects for utilization of these parents for the improvement of bunch production at the young age, the reduction of the vertical growth and the diversification of sources of vascular wilt tolerance of populations used in the reciprocal recurrent selection scheme were discussed.
      PubDate: 2014-08-14
      Issue No: Vol. 2, No. 2 (2014)
       
  • NUCLEAR DNA CONTENT ANALYSIS OF FOUR CULTIVATED SPECIES OF YAMS (DIOSCOREA
           SPP.) FROM CAMEROON

    • Authors: Marie F. Sandrine, Simon Joly, Mickaël Bourge, Spencer Brown, Denis N. Omokolo
      Pages: 87 - 95
      Abstract: Yam (Dioscorea spp.) is an important food source in Africa, but diseases and storage pests hinder the African farmers to achieve high yields during the harvest. One important limitation to the genetic breeding of yam is the relatively unknown ploidy level variation within and among species.  The objective of this study was to determine the nuclear DNA content of 59 accessions representing four cultivated Dioscorea species collected from three regions of Cameroon (Adamawa, Centre and Southwest) using flow cytometry with propidium iodide staining. Our findings suggested the variation of the genome size both within and among the yams species. Nuclear DNA content (mean 2C-value) in studied yam collection ranging from 0.72 ± 0.013 pg in D. dumetorum to 2.801 ± 0.068 pg in D. cayenensis. The accessions could be divided into four different categories according to their, nuclear DNA content suggestive of four different ploidy levels. Ploidy variation was observed within all species with the exception of D. dumetorum that is likely diploid. This study contributes to a better understanding of the genome characteristics of yam species from Cameroon and may help to the genetic improvement of this important crop in the future.
      PubDate: 2014-08-14
      Issue No: Vol. 2, No. 2 (2014)
       
  • MAINTAINING THE EFFICIENCY OF MAS METHOD IN CEREALS WHILE REDUCING THE
           COSTS

    • Authors: Odile Moullet, Arnold Schori
      Pages: 97 - 100
      Abstract: Marker-assisted selection (MAS) is a powerful tool too rarely exploited in practical breeding applications mainly because of its prohibitive costs. A new manual protocol has been developed for DNA extraction and polymerase chain reaction (PCR) analyses which could increase the impact of this technology on the creation of new varieties. In this procedure, only the amount of DNA serving as template is extracted directly into PCR tubes. The method is reproducible (100 %) and efficient (97.9 %). The overall cost is low in term of starting lab equipment (25000 €), chemicals and consumable materials (0.33 to 0.40 € per samples) and labor (1500 sample analyses per person and per week).
      PubDate: 2014-08-14
      Issue No: Vol. 2, No. 2 (2014)
       
 
 
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