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International Journal of Applied Sciences and Biotechnology
   [3 followers]  Follow    
  This is an Open Access Journal Open Access journal
     ISSN (Print) 2091-2609
     Published by Nepal Journals Online Homepage  [61 journals]
  • Anticariogenic Activity and Haemolytic Study of Some Medicinal Plants Leaf
           Protein Extract against Six Oral pathogens in In vitro condition

    • Authors: Hiral Soni, Kalpesh Ishnava, Khushal Patel
      Abstract: Leaf extract of 12 medicinal plants were obtained by using 10mM sodium phosphate buffer and studied for their antibacterial activity against 6 cariogenic pathogens. Among them, the extract from Streblus asper showed the most intensive activity. Antibacterial activity of Streblus asper was investigated by the minimum inhibitory concentration (MIC) test and the viable cell count method. MIC of Streblus asper against Candida albicans causing dental caries was determined to be 2.5 μg/ml. The kinetic study showed that Streblus asper completely inactivated C. albicans at the concentration 50μg/ml in 15hours.Hemolytic study of the Streblus asper plant leaf protein gives HC50 value at a very high range  than that of the MIC values against C. albicans of the organisms and thus flourish its application as successful pharmaceutical drug in practice.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 253-259   
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Wild Edible Plants Used by Endangered & Indigenous Raji Tribe in
           Western Nepal

    • Authors: Lal B Thapa, Til Maya Dhakal, Raghunath Chaudhary
      Abstract: The Rajis are one of the endangered indigenous people distributed in western part of Nepal enriched in their own mother tongue, culture, beliefs and practices. Owing to lack of proper documentation, the traditional knowledge of uses and practices on wild edible plants by such an endangered community is about to extinct. This paper aims to present the traditional practices and use of wild edible plants by Raji people in Nepal. Our study found that a total of 67 wild edible plant species included in 56 genera and 38 families used by Raji people. Out of them 62 species were angiosperms, one species was Gymnosperm and 4 species were Pteridophytes. The results of study show that Rajis have their traditional way to use different parts of wild plants such as seeds, fruits, leaves, shoots, roots and tubers in the forms of vegetables, pickles, juice, and raw or as fruits.
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 243-252   
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Correlation between Childhood Food and Physical Activities on the Old Age
           Health in Sangli District

    • Authors: V.V. Koshti, K.V. Ashokan
      Abstract: The food in childhood determines the health in old age. We analyzed the difference in the food habit and physical activities of urban and rural children and their effect in the development of old age diseases. The consumption of breakfast is more in urban area than rural children, similarly the consumption of meals is more in rural children comparing to urban one and the consumption of dinner is more in urban than rural one. The leafy vegetable and non-leafy vegetable consumption is more by rural children as compared to urban, but underground stem consumption is more by urban children than rural one. Fish and mutton consumption is also more in the case of urban children as compared to rural children. The consumption of chicken is less in urban children as compared to rural children. This difference in the underground stem and non-vegetable food consumption may be reason behind the excess weight gain and more old age diseases in the urban area. These facts must be cross checked with other factors for better interpretation. Our study indicates that urban children involved in physical activities less time comparing to rural one (51, 37 minutes) respectively for less than 30 minutes. But rural people involved in physical activities greater than 1 hour or two hour is more. This shows that rural children getting better physical work than the urban counterpart. This difference in physical activity will correlate the old age disease. Therefore to prevent various diseases in old age and provide better old age life a well planned awareness program must be initiated in all the preprimary and primary educational institutes without delay
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 238-242  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Marker Assisted Selection for Bacterial Leaf Blight Resistance in
           Segregating Populations of Cottondora Sannalu

    • Authors: Manu Maya Magar, Ch.V. Durga Rani, G Auradha
      Abstract: The present investigation was undertaken with the objective to develop high yielding, fine grain rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz., xa13 and Xa21 from B95-1. A popular high yielding and fine grain rice variety, MTU1010 (Cottondora Sannalu), susceptible to BLB and B95-1 carrying resistant genes for BLB (xa13 & Xa21 genes) was selected as the parent for crossing. B95-1 was verified for the presence of target genes by using gene linked primers viz., xa13 promotor and pTA 248. These primers were also used to study polymorphism between resistant (B95-1) and susceptible (MTU1010) parents. The cross viz., MTU1010 x B95-1 was affected during Rabi, 2010 and F1 progenies were confirmed during Kharif, 2011. The F1 plants confirmed as true hybrids for both the genes were advanced to F2 generation and foreground selection was done using gene linked markers. Genetic analysis in F2 populations confirmed that the genes (xa13 & Xa21) governing BLB resistance followed Mendelian inheritance. The phenotypic data analysis revealed that the plants carrying two resistance gene combinations (xa13xa13 Xa21Xa21, xa13xa13Xa21xa21) showed BLB resistance (0-2 scale), while the gene combinations viz., Xa13Xa13Xa21Xa21, Xa13Xa13Xa21xa21, and Xa13xa13Xa21Xa21, Xa13xa13Xa21xa21 showed BLB resistance (0.5-3.0).
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 229-237   
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Use of Computational Matrix Adjustment to Evaluate the Effectiveness of
           Common Influenza Vaccines against the Emergence of Drift Variants

    • Authors: Jennifer Tram
      Abstract: Every year the FDA issues a recommendation for the composition of the year’s common influenza vaccine for influenzas A and B.  The FDA can consistently predict the dominance of a particular strand of influenza virus by taking into account previous years’ antigenic characterization percentages. However, the sudden disappearance of dominant antigens and the sudden emergence of drift variants can disrupt this pattern, which questions the effectiveness of that year’s vaccine. Basic Local Alignment Search Tool was used to compare the protein sequences for hemagglutinin and neuraminidase between the strands in the vaccine and the dominant viral strands. This study examined the effectiveness of vaccines from 2000 to 2012, focusing on the transitions between the B/Yamagata and B/Victoria lineages and A/New Caledonia and A/California lineages (H1N1). Between the years 2005 and 2006, dominance of the B/Yamagata lineage, represented by B/Shanghai/361/2002, disappeared almost entirely. For the 2005-2006 flu season, the CDC recommended a B/Shanghai/361/2002 vaccine which expressed a 98% identity to the dominant influenza B hemagglutinin sequence and a 97% identity to the dominant neuraminidase sequence. From 2007 to 2008, the A/New Caledonia virus declined to 34% of cases while the A/Solomon Islands/3/2006 virus increased to 66%. The A/New Caledonia/20/99 vaccine effectively expressed a 97% identity to the hemagglutinin sequence of A/Solomon Islands/3/2006 strand and a 98% identity to the neuraminidase sequence. This study demonstrates that from 2000 to 2012, despite drift variants in influenza viruses, the CDC-recommended vaccine effectively matches the hemagglutinin and neuraminidase protein sequences of the dominant viruses.
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 224-228  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Harmony in Chaos: Protein Crystallography

    • Authors: Saurabh Kumar Singh
      Abstract: No abstract
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 222-223  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Studies in Growth Parameter and Pigment Contents in Endosulphan, EC- 35
           Exposed Blue Green Alga (Anabena Cylindrica, L.)

    • Authors: Goutam Sabat, A.K. Panigrahi, A.K. Sahu, Bijaya Kumar Mohanty
      Abstract: Anabaena cylindrica L, a blue green alga commonly found in rice fields was experimentally exposed with a board spectrum insecticide, Endosulphan, EC50 in laboratory conditions for varied days. The growth of alga in terms of increase in Absorbance, dry weight and pigments like Total chlorophyll, carotenoid and phaeophytin were estimated to study the toxic effect and the subsequent recovery from insecticide stress. The results obtained indicated decrease in all studied parameters with increase in exposure period and concentration. The highest concentration (3.25ml/L) and highest period (15 Days) severely affected the growth of alga and decreased pigment content. However, when the algae were allowed to grow in insecticide free environment for 15 days, they could able to recover fully from the toxic stress.
      DOI :  Int J Appl Sci Biotechnol, Vol. 2(3): 260-264 
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Soil Properties Affected by Land Use Systems in Western Chitwan, Nepal

    • Authors: R.P. Chauhan, K.R. Pande, S. Thakur
      Abstract: Field experiments were conducted in acidic soils of Mangalpur and Fulbari VDCs in western Chitwan, Nepal to study the effects of different land use systems on soil properties. Seven land use systems (cereal based lowland, cereal based upland, vegetable farm land, fruit orchard land, pasture land, forest land and farmer’s field) were used and they were replicated four times in randomized complete block designs. Composite soil samples were collected from each study sites and were analyzed in laboratory for soil physicochemical properties. The data obtained were analyzed using MSTAT-C. Soil properties were significantly affected by land use systems in western Chitwan condition. Soil organic matter and total soil nitrogen were significantly higher from pasture land (4.69 % and 0.23 %) and the lowest were from farmer’s field (2.40 % and 0.08 %). However, available soil phosphorous content was significantly higher from cereal based upland (448.3 kg ha-1) and it was the lowest from forest land (13.0 kg ha-1). Soil bulk density and pH were not significantly affected by land use systems. Since land use systems and management practices significantly affect soil physical and chemical properties, an appropriate and sustainable land use management option is necessary for fertile and healthy soil. Conservation tillage with the addition of sufficient organic inputs can be suggested based on this study to maintain soil health for sustained production and optimum activity of soil organisms under the western Chitwan land use systems.
      DOI :  
      DOI : Int J Appl Sci Biotechnol, Vol. 2(3): 265-269  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Distribution and Seasonal Abundance of Potato Tuber Moth: Phthorimaea
           operculella (Zeller) (Lepidoptera: Gelechiidae) in Nepal

    • Authors: Y.P. Giri, R.B. Thapa, N. Dangi, S. Aryal, S. M. Shrestha, S. B. Pradhan, M. Sporleder
      Abstract: Annual monitoring of potato tuber moth (PTM) was made in 2008/09 in15 districts of Nepal to understand the distribution and seasonal abundance. The locally made pheromone trap was installed and observed in 24 hours intervals. The activity of PTM was observed in plain, mid hills (below 2200 masl) and high hill districts. The population of PTM was found more in mid-hills than in plain whereas PTM was absent in high hill districts. The highest average number of PTM was observed in May (480±238 moth/month) with no adult moths in October to December in plain whereas 522±174 moth/month was observed in July and 18±4 moth/month in December in mid-hills of Nepal. The seasonal abundance of PTM observed from March to July (74±63 to 126±100 moth/month) in Plain and March to October (191±157 to 104±60 moth/month) in mid-hills. The understanding of PTM population dynamics could be useful to make suitable management decision.
      DOI :  Int J Appl Sci Biotechnol, Vol. 2(3): 270-274   
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Weeds and Their Effect on the Performance of Maize and Finger Millet in
           MID - Hills of Nepal

    • Authors: Tika Bahadur Karki, Shrawan K. Sah, Resam B. Thapa, Andrew J. McDonald, Adam S. Davis, Yajna G. Khadka
      Abstract: Relay cropping of maize with fingermillet (maize/fingermillet) is the predominant cropping system for sustaining food security situation in the hilly regions of Nepal. In this region weed pressure severely reduces crop yields. Basic information on weed species composition, biomass production and their effect on crop yields and economics are lacking for this region. This information will be necessary to develop effective weed management strategies for the future. In light of this an empirical study was carried out in two representatives mid hill districts of Parbat and Baglung during summer season of 2010/2011 in Nepal. A total of 10 major weed species with densities of 172 in Parbat and 311 per 0.25m2 area in Baglung were observed. The highest percentage of both relative and absolute densities were recorded for Ageratum conyzoides in Parbat and Polygonum chinensis in Baglung. Weed infestation under farmers practice of crop management reduced the grain yield of maize by 1.985 Mt ha-1 (117%) in Baglung and 1.760 Mt ha-1 (108%) in Parbat. Similarly, in finger millet it was 0.489 Mt ha-1 (63%) in Baglung and 0.403 Mt ha-1 in Parbat. Similarly, the combined yield of both the crops was also significantly reduced by 79.3% and 61.7% in Baglung and Parbat respectively. Hence, weeds are directly affecting the crop performance in the region. Therefore, there is an urgent need to develop an alternative crop production system in the hills.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 275-278  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Electrophoretic Protein Banding Patterns among Penicillium Strains
           Isolated from Saudi Arabia

    • Authors: Ehab Abdel-Razik Kamel, M. Elsayed Rashed
      Abstract: 14 strains of Penicillium species were isolated from different localities and habitats from Jeddah, Saudi Arabia and cultivated on two different media: Czapek Dox’s medium, in which NaNO3 is the source of inorganic nitrogen, and Waksman’s medium, in which pepton is the source of organic nitrogen. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) technique was used in this study to distinguish these isolates. The Penicillium isolates examined in this study consisted of six Penicillium species: Penicillium corylophilum (three isolates), P. rubrum (one isolate), P. citrinum (two isolates), P. crustosum (one isolate), P. canesens (six isolates) and Penicillium sp. (one isolate). The electrophoretic protein patterns from Penicillium isolates grown on Czapek Dox's medium revealed the presence of 17 different bands (out of 14 polymorphic bands, there were three monomorphic bands and two unique bands). The electrophoretic protein pattern of the same isolates grown on Waksman's medium revealed the presence of 12 different bands (out of eight polymorphic bands, there were four monomorphic bands and one unique band). Data were analysed by a clustering method and similarity coefficients using NTSYSpc version 2.02i. Two different phenograms were produced for the studied Penicillium species based on the analysis of the protein banding patterns. Data from the protein banding patterns produced from both media were combined and analysed to produce third phenogram, and the relationships between the species and isolates are discussed.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 283-290  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Effect of Fermentation on Nutritional Composition of Selected Commonly
           Consumed Green Leafy Vegetables in Nigeria

    • Authors: B.O.T. Ifesan, O.O. Egbewole, B.T. Ifesan
      Abstract: Fresh leafy vegetables which include Amarathus hybridus (Bush green), Telfairia occidentalis (Fluted pumpkin leaf), Vernonia amygdalina (Bitter leaf) and Pterocarpus mildbraedii (White campwood leaf) were subjected to spontaneous fermentation for 5 days at room temperature. Physicochemical properties (pH, temperature, and total titratable acidity) were monitored during the fermentation. It was observed that the pH (6.8 to 3.78) of all the vegetables decreased steadily as fermentation progressed. However, the temperature (27°C to 34°C) and total titratable acidity expressed as lactic acid equivalent (0.014-0.147) for all the vegetables increased as fermentation progressed. The percentage of occurrence of bacteria isolated from the fermenting mass include Bacillus spp. (39.1%), Lactobacillus spp. (26.1%), Escherichia coli and Staphylococcus spp. (8.7%), Proteus spp., Serratia spp., Citrobacter spp. and Pseudomonas spp (4.3%) respectively. The proximate composition and mineral contents of fermented and unfermented leaves were investigated. Significant increase in ash content was noticed for unfermented (8.07%-15.72%) and for fermented vegetables (12.50%-23.28%). The protein contents of T. occidentalis (24.29%-25.65%), and A. hybridus (14.27%-16.96%) increased significantly after fermentation. A decrease in fiber content was noticed for all fermented vegetables except for V. amygdalina where significant increase was noticed (10.97%-14.55%). Increase in both thiamine (1.37-1.52mg/ml) and niacin (1.32-1.62mg/ml) contents were observed in T. occidentalis after fermentation while significant reductions were recorded for A. hybridus and V. amygdalina. Generally, vitamin C content of the vegetables decreased during fermentation while some increases were recorded in the mineral content of fermented vegetables.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 291-297  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Response of Tillage System, Nitrogen Level and Split Application of
           Nitrogen on Spring Maize in Chitwan, Nepal

    • Authors: B. Pandey, N.K. Chaudhary
      Abstract: Field experiment was conducted at Phulbari-9, Chitwan, Nepal during 2012 (Feb-July) to investigate response of tillage system, nitrogen level and split application of nitrogen on spring maize. The experiment was laid out in split-split plot design with twelve treatments and three replications, considering two tillage system (conventional and zero tillage) as main factor, three nitrogen levels (60, 120 and 180 kg N ha-1) as sub plots and spilt application of nitrogen (50 % each at basal and 45 DAS & 25% each at basal, 30, 45 and 60 DAS) as sub sub plot. Thousand grain weight, number kernel rows ear-1, number kernels ear-1, grain yield, stover yield and harvest index were significantly not influenced by tillage systems. Thousand grain weight, number of kernel rows ear-1, number kernels ear-1, grain yield, stover yield as well as harvest index showed remarkable increase with increasing N rate and number of N split application. Significantly, higher grain yield (8.91 t ha-1) was observed in 180 kg N ha-1 applied plot than the plots given 120 kg N ha-1 (8.15 t ha-1) and 60 kg N ha-1 (5.29 t ha-1). Four equal split application of nitrogen yielded significantly higher grain (7.68 t ha-1) than two equal split application of nitrogen (7.22 t ha-1). It is concluded that either conventional or zero tillage with application of 180 N kg ha-1 with four split application can increase yield and yield attributes.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 298-301  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Assessment of Physico-Chemical Parameters of Soil of Muthannan Kulam
           Wetland, Coimbatore, Tamil Nadu, India

    • Authors: K Manimegalai, S Sukanya
      Abstract: Soil serves as a more reliable index for productivity than water qualities. The productivity of any pond depends largely on the quality of bottom soil that is “store house of nutrients.” The present paper deals with the study of physicochemical parameters like organic carbon, pH, Electrical conductivity, Nitrogen, Phosphorus, Potassium of soil from Muthannan Kulam,Coimbatore (March 2013- May 2013). Analysis for the above parameters indicated contamination of the soil quality due to some anthropogenic activities.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 302-304  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Green Synthesis of Silver Nanoparticles from Stem Extracts of Caralluma
           Fimbriyata and Its Antibacterial Activity

    • Authors: N. Packialakshmi, S. Naziya
      Abstract: The aim of this study was to synthesis of silver nanoparticles in the aqueous stem extracts of Caralluma fimbriyata and investigate its antibacterial activity. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous stem extracts of plant parts to generate to extremely stable silver nanoparticles in water. The chemical groups studied using FT-IR analysis. Green synthesized silver nanoparticles showed zone of inhibition against isolated gram positive and gram negative bacteria.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 305-310  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Pig Sero-Survey and Farm Level Risk Factor Assessment for Japanese
           Encephalitis in Nepal

    • Authors: Shristi Ghimire, Santosh Dhakal, Narayan Prasad Ghimire, Durga Datt Joshi
      Abstract: The objectives of this cross-sectional study was to determine the seropositivity status of Japanese encephalitis virus (JEV) in pig and ascertain the farm level risk factors in Nepal. Serum samples were collected from 181 pigs in 91 farms of Rupandehi and Kapilbastu districts and tested for antibodies by IgG ELISA. Survey of farmers in each farm was carried out to determine the farm level risk factors. A total of 20 out of 181 (11%) serum samples tested positive for JEV antibodies. There was no statistically significant difference in seropositivity with age (p>0.05), sex (p>0.05) and breed (p>0.05) of pigs. Farm level infection was significantly associated with closeness to rice fields (p<0.05), closeness to standing water sources (p<0.05), wild bird exposure (p<0.05) and mosquito bite to pigs (p<0.05). These factors need to be addressed while formulating prevention, control and management strategies of JEV in pig and hence in human.
      DOI :  Int J Appl Sci Biotechnol, Vol. 2(3): 311-314  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle)
           Landraces of Eastern Nepal Using RAPD Markers

    • Authors: NN Munankarmi, RL Shrestha, N Rana, JKC Shrestha, S Shrestha, R Koirala, S Shrestha
      Abstract: Acid lime (Citrus aurantifolia Swingle) is an important commercial fruit crop, cultivated from terai to high hill landscapes of Nepal. However, production and productivity is very low due to various reasons including infestations by various diseases and pests, lack of diseases and pests resistant and high yielding varieties. In this context, determination of genetic variation at molecular level is fundamental to citrus breeders for the development of elite cultivars with desirable traits. In the present study, Random Amplified Polymorphic DNA (RAPD) marker technique has been employed to assess genetic diversity in 60 acid lime landraces representing different agro-ecological zones of eastern Nepal. Nine selected arbitrary primers generated 79 RAPD fragments of which 75 were polymorphic (94.94%). Phenogram was constructed by NTSYSPC ver. 2.21i using UPGMA cluster analysis based on Jaccard’s similarity coefficient to deduce overall genetic diversity and relationships of the acidlime genotypes under study. Sixty acid lime landraces formed seven clusters and similarity value ranged from 38% to 98% with an average of 72%. Genetic variation at different agro-ecological zones was assessed using Popgene ver. 1.32 and found 47% to 69.6% polymorphism. Shannon’s index and Nei’s gene diversity showed highest level of acid lime diversity in Terai zone (PPB, 69.62%; H, 0.213; I, 0.325) followed by mid-hill zone (PPB, 67.09%; H, 0.208; I, 0.317). The results obtained will be useful to citrus breeders for elite cultivar development. The RAPD-PCR technique is found to be the rapid and effective tool for genetic diversity assessment in acid lime landraces of Nepal.
      DOI :  Int J Appl Sci Biotechnol, Vol. 2(3): 315-327  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Tillage, Residue, Fertilizer and Weed Management on Phenology and Yield of
           Spring Maize in Terai, Nepal

    • Authors: Saugat Dahal, Tika Bahadur Karki, Lal Prasad Amgain, Birendra Kumar Bhattachan
      Abstract: With the aim of developing crop management technologies that reduce the yield gap of maize (Zea mays L.) in Nepal, a study was carried-out to determine whether the grain yield of maize could be manipulated through tillage, residue, and nutrient and weed management practices. The effect of tillage (conventional and no tillage), residue (residue retained and residue removed), fertilizer (recommended doses of fertilizer and farmers’ doses of fertilizer) and weed management practices (herbicide use and manual weeding) on phenology and grain yield of maize were investigated under maize-rice cropping system in Rampur, Nepal during 2013. The experimental results revealed that no tillage had significant effect on grain yield (6.64 Mg ha-1) and phenological parameters like days to silking, physiological maturity and seed fill duration. Similarly, residue retained treatment had significant effect on grain yield (7.02 Mg ha-1) and phenological parameters. Research dose of fertilizer had significant effect on phenological parameters and grain yield (8.42 Mg ha-1). However, weed management factor did not influence significantly on grain yield and phenological parameters. The grain yield increased in no tillage by 23.19% over conventional tillage, residue retained by 39.84% over residue removed, recommended doses of fertilizer by 132.60% over farmer dose of fertilizer. Thus, no tillage, residue retention, recommended doses of fertilizer and use of herbicide for weed management can be alternative technologies for sustainable higher grain yield.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 328-335  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Study of the Exposure Rate from the Patients Injected with

    • Authors: O. B. Khanal, D. Thapa, B. R. Shah, D. D. Mulmi, A. Shah, K. Adhikari, S. K. Aryal
      Abstract: Nuclear medical imaging is done by injecting very small amount of radiopharmaceutical to the patient. The radiations from patients are detected by special type of Gamma camera that works with computer to yield precise pictures of the organs being imaged. The Technetium-99m, injected to renal scan patient, is the source of radiation to the individual near to patient. Thus the individual receives exposure from the patient. The exposure received by public in the imaging center from renal scan patients has been calculated. The exposure rate at the center is measured to check whether an individual near to the patient is below the internationally acceptable public dose limit. Public receive low amount of average exposure of (3.7 ± 1.7) mSv/h at 1m distance for delayed scan. However, public receives high amount of exposure of (234.4 ± 74.8) mSv/h at shorter distance of 0.25 m after immediate scan are not subject to dose limits of the occupational radiation worker. The exposure received at shorter distance is higher from the patients. Thus the hospitals providing radiation treatments must take account of the public dose limit for individuals accompanying treated patients.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 336-341  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Design, Synthesis and Screening of Newer-8-Hydroxy Quinoline Derivatives
           as Novel Anti Tubercular Agents

    • Authors: S R Surekha, Lincy Joseph, Mathew George
      Abstract: A series of 8-hydroxyquinolines are synthesized and their Antitubercular, antifungal, and antibacterial activities are tested. Quinolines are found to possess antibacterial, antifungal, immunosuppressive, analgesic, vasorelaxing, Antiplasmodial, anticancer and PDE4 inhibitory activities. Aryloxypropanolamines were reported to be associated with β-adrenergic blocking, CNS depressant and hypotensive activities. In view of the potential nature of these moieties it was considered worthwhile to study the effects of two pharmacophoric moieties such as quinoline and propanolamines/amino ethane in a single molecule. In the present study the synthesis, antitubercular, antifungal, and antibacterial activities and structure activity relationship of aryloxypropanolamine substituted 8-hydroxy quinolines are reported. The compounds were characterized by IR, H-NMR, spectral and Elemental analysis.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 342-358  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Nutritional Evaluation of Some Fortified Traditional Weaning Meals from
           Oriade Local Government Area of Osun State, Nigeria

    • Authors: F.A. Bamisaye, Emmanuel Oladipo Ajani, I.O. Nurain, J.B. Minari
      Abstract: Nutritional status of traditional weaning meals used in Oriade Local Government Area of Osun State Nigeria, were evaluated. Five different fortified weaning meals were labeled as Red beans based diet (RBD), White beans based diet (WBD), Groundnut based diet (GND), Soya beans based diet (SBD) and Sorghum based diet (SGD) were used to feed five groups of young rats of three weeks old. The initial weights of rats were noted and thereafter maintained on their respective diet for twelve weeks. The final body weights of the rats were also noted after the twelve weeks of experiment. The rats were then sacrificed and their liver, heart and kidney weights were noted and compared. The results revealed that soya beans based diet produced the best growth compared with other formulas and control. However, this study concluded by advising nursing mothers to complement these weaning formulas with one another to wean their babies since, if prepared hygienically may improve nutritional status of their babies.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 359-363  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Variability Study of Advanced Fine Rice with Correlation, Path
           Co-efficient Analysis of Yield and Yield Contributing Characters

    • Authors: M. A. Rahman, M. S. Hossain, I.F. Chowdhury, M.A. Matin, H. Mehraj
      Abstract: The experiment was conducted in experimental Farm, Sher-e-Bangla Agricultural University, Bangladesh from July to December 2011 to study the variability of advanced lines of fine rice with correlation and path co-efficient of yield and yield contributing characters. Seven newly developed lines viz. S-1, S-2, S-5, AL-33(II), AL-36, AL-42(II), AL-44(I) and Chinigura. Grain yield/plant of newly developed advanced fine rice lines and check showed positive association in respect of number of effective tillers/hill (0.308), number of filled spikelets/panicle (0.110) and weight of 1000-grains (0.109), whereas significant negative association with panicle length (-0.609), number of unfilled spikelets/panicle (-0.542) and non significant negative association with plant height (-0.136) and number of ineffective tillers/hill (-0.304). Path analysis revealed that plant height had positive direct effect (0.154), number of effective tillers/hill had positive direct effect (0.065), number of ineffective tillers/hill had negative direct effect (-0.114), panicle length had positive direct effect (0.163), number of filled spikelets/panicle had positive direct effect (0.285), number of unfilled spikelets/panicle had negative direct effect (-0.154), weight of 1000-grains had positive direct effect (0.234) on grain yield/plant.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 364-370  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Peritoneal Tuberculosis in Pregnancy: A Rare Occurrence

    • Authors: Priti Anand, Mukul Bindra
      Abstract: Extrapulmonary tuberculosis is least common form of tuberculosis in pregnancy, early diagnosis of which will help to reduce maternal and neonatal morbidity and mortality. My patient is 25 years old primigravida admitted with Ascitis, Anorexia, low grade fever on and off. Diagnosed as peritoneal tuberculosis after abdominal fluid sent for AFB culture. Patient was given Antitubercular treatment. Ascitic fluid gradually declined and pregnancy moved on but patient landed up in preterm labour and delivered baby which the pediatrician was unable to save because of extreme prematurity.
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 371-372  
      PubDate: 2014-09-25
      Issue No: Vol. 2 (2014)
  • Antibacterial Screening on Leaves of Argyreia Cymosa Roxb. Against
           Pathogenic Bacteria Isolated from Infected Pateints Samples Wound, Sputum
           and Stool

    • Authors: N. Packialakshmi, H. Fazila Beevi
      Abstract: The present study deals with the aqueous leaf extract and synthesized silver nanoparticle of Argyreia cymosa (Roxb) were evaluated for antibacterial activity. The aqueous leaf extract and synthesized nanoparticle of Argyreia cymosa is active against E. coli, P. aeruginosa, Bacillus and S. aureus. The aqueous leaf extract showed maximum activity against Bacillus (20mm), S.aureus (18mm), P. aeruginosa (13mm) E.coli (12mm) and the synthesized silver nano particle showed maximum activity against Bacillus (27mm), E. coli (21mm) , S. aureus (12mm) and P. aeruginosa (11mm).
      DOI : J Appl Sci Biotechnol, Vol. 2(3): 279-282  
      PubDate: 2014-09-07
      Issue No: Vol. 2 (2014)
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