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Journal Cover International Journal of Applied Sciences and Biotechnology
  [1 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2091-2609
   Published by Nepal Journals Online Homepage  [74 journals]
  • Indigenous Peoples, Indigenous Knowledge and Their Issues on Climate
           Change, particularly on REDD+, in Developing Countries

    • Authors: Shree Kumar Maharjan, Keshav Lall Maharjan
      Pages: 273 - 283
      Abstract: The lands, territories and resources of indigenous peoples (IPs) have been of significant historical importance to their livelihoods, cultures and spiritual identities. Over 370 million indigenous peoples are currently living in 70 countries in the world, mostly in remote, ecologically fragile and marginalized areas that are rich in traditional skills, knowledge and practices. Climate change is a global issue, impacting all livelihood sectors at the national and local levels. The poor and marginalized people, especially indigenous peoples, Dalits and women are in the forefront of its vulnerability and impacts. These people and communities have made the lowest contributions to the greenhouse gases emissions, however, they are the most at risks to its impacts. Furthermore, the policies and programs often marginalize and sideline the voices and issues of these people. This paper attempts to gather, review and analyze the information on indigenous knowledge, issues of indigenous peoples on climate change particularly in REDD+ in developing countries. The paper aims to highlight the REDD+ process in Nepal, its obligations at the national and international level (with cases from Asia, Africa and Latin America) and the indigenous perspectives towards the issues, gaps and challenges within the national and international negotiations.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 273-283
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18293
      Issue No: Vol. 5, No. 3 (2017)
  • An Evaluation of the Protein Digestibility of Flours and Derived Protein
           Rich Product of Three Varieties of Mucuna Pruriens (L.) From Rats (Rattus
           Norvegicus) Males in Growth

    • Authors: Maoudombaye Théopihile, Mahamat Seid Ali, Gomoung Doloum, Ngatchic Metsagang Therese Josiane, G. Ndoutamia
      Pages: 284 - 292
      Abstract: The objective of the present study is to evaluate the proteinaceous digestibility of crude flours and derived protein rich product of three varieties of Mucuna pruriens of the male albino rats in growth. 48 rats have been taken randomly split up into 8 groups where 6 each according to their formulated feedings; those foods are: foods without protein (RSP), foods with casein (RC), food with the black macuna derived protein rich product (RMNc), food with the scratch macuna derived protein rich product (RMRc), food with the white macuna derived protein rich product (RMBc), food with the black macuna crude flours (RMNf), food with the scratch macuna crude flours (RMRf) et food with the white macuna crude flours (RMBf). All those foods content the same quantity of azote excepted foods without protein. Rats have been isolated in the semi metabolic cages which allow gathering the remaining of the foods supplied and the faeces to determine the parameter values of consumption. The experiment has taken 28 days. The apparent and reel protein digestibilities obtained in each food are respectively : RC (76,63±0,36% ; 95,86±0,06%), RMNc (71,20±0,77% ; 91,60±0,99%), RMRc (73,68±0,23% ; 93,49±0,85%), RMBc (71,16±0,59% ; 92,03±0,70%), RMNf (33,25±1,91% ; 53,881±1,70%), RMRf (31,3±2,40% ; 52,12±2,22%) and  RMBf (31,12±4,00% ; 51,92±3,8%). The results show that derived protein rich product have ameliorated the protein digestibility and could be the alternative for the use of seeds of Mucuna pruriens to feed human beings and animals.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 284-292
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18119
      Issue No: Vol. 5, No. 3 (2017)
  • Isolation of Ethidium Bromide Degrading Bacteria from Jharkhand

    • Authors: Anil Kumar, Preeti Swarupa, Vikram Pal Gandhi, Snehal Kumari
      Pages: 293 - 301
      Abstract: Ethidium bromide (EtBr) is a carcinogenic and mutagenic agent which is widely used in research laboratories to probe nucleic acids by gel electrophoresis. It is generally buried underground (for solid waste) or disposed of pouring it down the sink (in case of liquid waste). Soil or drain microbial community may be able to take care of such substance else it will lead to contamination of our underground resources or others through defined and undefined routes. In view of the above assumption and literature reports the present study was undertaken to isolate and evaluate bacteria for removal, by bioaccumulation and /or biotransformation, of EtBr from contaminated sources and wastes, before their disposal to the environment. Two distinct bacteria both motile BR3 and BR4 could be identified from agarose-gel-waste containing 0.5-1.0 mg/ml ethidium bromide. Both bacteria were found to grow on EtBr-NA plate (Nutrient-Agar supplemented with EtBr at a concentration of 30 mg/ml) however only BR3 isolate showed large non-fluorescent-halo zone (characteristic to degradation of EtBr) when exposed to trans-UV light. Other isolate BR4 could accumulate EtBr within the colony biomass but did not showed clear (non-fluorescent) hallow zone around it. However the bacterium is not able to utilize the EtBr as a sole carbon source.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 293-301
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18296
      Issue No: Vol. 5, No. 3 (2017)
  • Incidence of Multi Drug Resistance and Diversity of TEM-1 Beta Lactamase

    • Authors: K. Natarajan, R. Subashkumar
      Pages: 302 - 308
      Abstract: Occurrence and incidence of drug resistant bacteria are becoming very high and common with overuse of antibiotics. This study focused on the isolation of drug resistant strains from the clinical and environmental sample that produce extended spectrum β-lactamase (ESBL) and identification of TEM-1 genes in the plasmid and genomic DNA. 45 Clinical samples from the hospital and 7 environmental samples from the polluted water sources were collected. Among the collected samples, E. coli showed a higher incidence (36.1 %) followed by Klebsiella sp., Staphylococcus sp., Streptococcus sp., Pseudomonas sp., Proteus sp., and Salmonella sp. Antibiotic susceptibility of the isolates were investigated against 25 commercially available antibiotics. All the isolated strains showed MAR index value of more than 0.2. Among the 36 isolates, 7 Gram negative isolates (19.4 %) showed positive results for ESBL production in Double disk synergy test. The plasmid and genomic DNA were isolated and analyzed using PCR with specific primers for the presence of the TEM-1 gene (716 bp). A selected PCR products of the TEM-1 gene was sequenced and analyzed using BLAST.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 302-308
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.17693
      Issue No: Vol. 5, No. 3 (2017)
  • Detection of hdc Gene from Histamine Producing Bacillus subtillis and
           Serratia marcescens

    • Authors: C. Aishwarya Laxmi, S. Munaliga, N.G. Ramesh Babu, R. Ragunathan
      Pages: 309 - 312
      Abstract: In the present study, two histamine producing bacteria were used to detect the presence of hdc gene using PCR. The histamine production was confirmed by using Soyabean Casein Digest Agar (Tryptone Soya Agar). The DNA was isolated using Phenol: chloroform method. PCR was carried out using hdc gene and the amplified product was confirmed (657bps and 668 bps respectively). Further, the DNA and the PCR product were transformed into E.coli and the gene transformation was confirmed.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 309-312
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.17826
      Issue No: Vol. 5, No. 3 (2017)
  • Emergence of the Epizootic Ulcerative Syndrome in Pond Reared Carp Fish
           (Cyprinids) and It's Control Measure in Chitwan, Central Nepal

    • Authors: Asha Rayamajhi, Swayam Prakash Shrestha, Bhagwat Prasad
      Pages: 313 - 320
      Abstract: A survey on epizootic ulcerative syndrome (EUS) was carried in carp fish (cyprinids) reared in ponds during January 2016 to January 2017 at six locations of Chitwan, Nepal. Outbreaks of EUS were observed in 39.6% of the carp during winter. EUS infection was the most for Cirrhinus mrigala (47.3% of the 170 samples) and the least for Labeo rohita (12.3% of the 65 samples). EUS infected fish weighing range between 15g-300g were characterized as extensive deep ulceration on head, dorsal and lateral part of body and at caudal peduncle region. Histopathology examination of affected tissue of skin with adjacent muscles showed mycotic granulomas indicated a positive diagnosis for EUS. Survey results indicate that poor water quality in undrained ponds, uncontrolled entry of flooded water into the pond, runoff water from adjacent paddy field and use of contaminated equipment and seine nets were found to trigger risk for EUS infection in carp pond. Impact assessment of EUS showed that average economic loss due to EUS fish disease was 2257.92 kg/ha/year (33.01%) of the expected production. The highest economic loss of 1432.14 kg/ ha/year (53.53%) was estimated for large farm and lowest loss of 950 kg/ha/year (5%) for medium size farm. The simultaneous application of Ciphalexin in feed at biomass of fish for 15 consecutive days and two applications of Kohrsolin-TH at 900 ml/ha (1 m pond depth water) at weekly interval was found effective to control EUS infection in carps. The combined efficacy of these two drugs was estimated to be 97.7% control of EUS infection in carp fish. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 313-320
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18019
      Issue No: Vol. 5, No. 3 (2017)
  • Prevalence of Haemonchus contortus in Goats of Western Chitwan of Nepal

    • Authors: Kashyap Adhikari, Hari Bahadur Rana, Krishna Kaphle, Tanka Khanal, Rabin Raut
      Pages: 321 - 325
      Abstract: The research was done in cross sectional design to study about the prevalence of Haemonchus contortus in goats of Western Chitwan of Nepal. A total of 252 fecal samples were collected through purposive sampling. Fecal samples were examined through sedimentation and floatation method. Egg per gram (EPG) was calculated through Mac Master Counting Technique of positive samples. Total prevalence was found to be 13.89%. Infection of parasite was more prevalent in males (15.18%) than in female (12.86%) but was statistically non-significant (P>0.05). In non-dewormed goats (40.32%), the prevalence was significantly (P<0.05) higher than in dewormed goats (5.26%). The results regarding the relationship between different age groups of goats and H. contortus showed that highest prevalence (14.54%) of parasite was observed in age group of below 1 year and the lowest (12.96%) in age group of  above 2 year, statistically non-significant (P>0.05). Maximum goats were found with medium level of infestation through EPG. This study shows higher prevalence of Haemonchus in non-dewormed than in dewormed goats which is statistically significant at (P<0.05). Thus, effective deworming programme and management must be maintained in order to upgrade the health status and maximize the benefits from the animal.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 321-325
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18268
      Issue No: Vol. 5, No. 3 (2017)
  • The Intensity-Curvature of Human Brain Vessels Detected with Magnetic
           Resonance Imaging

    • Authors: Carlo Ciulla, Ustijana Rechkoska Shikoska, Dimitar Veljanovski, Filip A. Risteski
      Pages: 326 - 335
      Abstract: The intensity-curvature term is the concept at the root foundation of this paper. The concept entails the multiplication between the value of the image pixel intensity and the value of the classic-curvature (CC(x, y)). The CC(x, y) is the sum of all of the second order partial derivatives of the model polynomial function fitted to the image pixel. The intensity-curvature term (ICT) before interpolation E0(x, y) is defined as the antiderivative of the product between the pixel intensity and the classic-curvature calculated at the origin of the pixel coordinate system (CC(0, 0)). The intensity-curvature term (ICT) after interpolation EIN(x, y) is defined as the antiderivative of the product between the signal re-sampled by the model polynomial function at the intra-pixel location (x, y) and the classic-curvature. The intensity-curvature functional (ICF) is defined as the ratio between E0(x, y) and EIN(x, y). When the ICF is almost equal to the numerical value of one (‘1’), E0(x, y) and EIN(x, y) are two additional domains (images) where to study the image from which they are calculated. The ICTs presented in this paper are able to highlight the human brain vessels detected with Magnetic Resonance Imaging (MRI), through a signal processing technique called inverse Fourier transformation procedure. The real and imaginary parts of the k-space of the ICT are subtracted from the real and imaginary parts of the k-space of the MRI signal. The resulting k-space is inverse Fourier transformed, and the human brain vessels are highlighted.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 326-335
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18271
      Issue No: Vol. 5, No. 3 (2017)
  • Finding a possible biomarker to tackle Parkinson’s disease by splice
           analysis and random point mutations to counter the expression of genes
           involved in Parkinson’s disease

    • Authors: Piyusha Kalwad, Ranjitha Guttapadu, Pradeep Shasi
      Pages: 336 - 344
      Abstract: The genes showing aberrant alternative splicing in Parkinson’s disease namely SNCA, SNCAIP, LRRK2, SRRM2, MAPT and PARK2 were analysed. Two of the genes, namely SNCAIP and SRRM2 that showed high effect were taken and splice site analysis was carried out. Random mutations were carried out on these two genes using Human Splicing Finder tool and the mutations showing the most promising results (i.e., mutations that can restore natural gene expression) were appropriately chosen to tackle Parkinson’s disease.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 336-344
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18290
      Issue No: Vol. 5, No. 3 (2017)
  • Perspective Response of Climate Change Impacts on Agricultural Crops in
           Sauraha-Pharsatikar VDC, Rupandehi District, Nepal

    • Authors: Kapil Khanal, Subodh Khanal, Surya Mani Dhungana
      Pages: 345 - 355
      Abstract: A survey research was conducted in Sauraha-Pharsatikar VDC of the Rupandehi district to study the perspective response of the farming communities on the impacts of the climate change in agricultural crops. Primary information was collected from household survey by administering pre-tested questionnaire and necessary data were collected from National Wheat Research Project (NWRP), Bhairahawa. Several results are obtained on the recall basis of the respondents thus they can not assumed correctly and all the past information provided by the farmers cannot be cross checked due to the lack of sufficient and reliable system for recording and checking. The trend analysis of rainfall data of Bhairahawa of 30 years (1984-2013) showed that the pattern of rainfall was irregular and it was in a decreasing trend by 1.944 mm per year and average maximum temperature has increased by 0.0.15oC and average minimum temperature has increased by 0.0.61oC per year which justifies that the summers are growing hotter and winters are growing warmer. About 52% of the respondents suggested monsoon starts earlier, 85% suggested there is more intense rain during the monsoon and 91.75% suggested drought has increased. 98.33% of the respondents perceived that the summer has become hotter. In general there is increase in the yield of cereal crops whereas the yield of pulses, legumes and vegetables had declined. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 345-355
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18291
      Issue No: Vol. 5, No. 3 (2017)
  • Quantification of Cry1Ac Protein in Bt Eggplant Fruits

    • Authors: M.M. Khatun, M.K. Hasan, M.K. Jamil, D. Khanam
      Pages: 356 - 360
      Abstract: The cultivation of genetically engineered crops has been expanded rapidly in worldwide in a very short span of time. Bt eggplant is a transgenic eggplant created by inserting a crystal protein gene Cry1Ac from the soil bacterium Bacillus thuringiensis. Four Bt eggplant varieties - BARI Bt Eggplant-1, BARI Bt Eggplant-2, BARI Bt Eggplant-3 and BARI Bt Eggplant-4 are started to cultivate in Bangladesh in 2014 as first genetically engineered crop in the country.  In the present study, ELISA technique was adopted to quantify the Cry1Ac proteins in the fruits of newly released four Bt eggplant varieties. The expression of Cry1Ac was found among the fruits of the varieties varied from 29.53 to 33.99 µg g-1. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 356-360
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18292
      Issue No: Vol. 5, No. 3 (2017)
  • Palynological Studies on Ten Species of Angiosperms from Nepal

    • Authors: Pushpa Karna Mallick
      Pages: 361 - 365
      Abstract: Palynological studies on ten species of angiosperms family namely Hibiscus rosa- sinensis L.(Malvaceae), Lilium candidum L. (Liliaceae), Petunia axillaris (Lam.) B.S.P.(Solanaceae) and seven species from family asteraceae namely Artemisia indica Willd., Aster ageratoides Kitam., Calendula offcinalis L., Cerpis japonica L. (Benth.), Sonchus asper (L.) Hill, Tagetus patula L., and Taraxicum officinale F.H.Wigg. was carried out. Result obtained from this investigation showed that pollen shape is spheroidal in Artemisia indica, Aster ageratoides, Calendula offcinalis, Hibiscus rosa- sinensis and P. axillaris; elliptical in L. candidum, hexagonal in S. asper and pentagonal in T. officinale; tricolpater in C.japonica. Pollen aperture is porate in all except in L.candidum where it is elliptic. Pollen wall is echinate in H.rosa-sinensis, A.ageratoides, T. patula; spinulate in P. axillaries, T. officinale, S. asper, C. japonica, C. officinalis and T. officinale and smooth in L. candidum. The general appearance of the pollen grain is circular in P. axillaries but longer than wide in L.candidum. The circular, echinate, large and triporate pollens seem to be primitive ones and spharoidal, hexaporate features have been regarded as comparatively advanced ones. Polyporate pollens are considered to be secondarily derived. The nature of the pollen grain in these species could be evolutionary significance. The similarities in structure of the pollen grain showed the inter- species relationships among the species and that’s why put in same family while the differences in structures showed reasons for them to exist as distinct species. Only one species namely L.candidum from monocot family (Liliaceae) included in this study. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 361-365
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18294
      Issue No: Vol. 5, No. 3 (2017)
  • Influence of Annealing on Creep Indentation, Surface Properties and
           Electrochemical Corrosion Behavior of Ni-Cr Based Dental Alloy

    • Authors: Abu Bakr El-Bediwi, Eman Kashita, Salah M.M. Salman
      Pages: 366 - 374
      Abstract: Heat treatment is a process in which alloy is heated to get different microstructures and desired properties associated with it which may affect the corrosion rate, nature form and size.  Effect of annealing on microstructure, creep indentation, hardness and electrochemical corrosion behavior of Ni63Cr24.6Mo10.8Si1.5Mn0.06C0.04 alloy were investigated.  Microstructure of Ni63Cr24.6Mo10.8Si1.5Mn0.06C0.04 alloy changed after annealing at different temperature for two hours.  Crystal size of nickel in matrix alloy increased after annealing for two hours at different temperature.  Stress exponent, n, Vickers hardness and calculated maximum shear stress of Ni63Cr24.6Mo10.8Si1.5Mn0.06C0.04 alloy decreased after annealing. Corrosion rate of Ni63Cr24.6Mo10.8Si1.5Mn0.06C0.04 alloy increased after annealing for two hours at different temperature. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 366-374
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18295
      Issue No: Vol. 5, No. 3 (2017)
  • Study of Fermentation Kinetics of Palm Sap from Cocos nucifera

    • Authors: Premalatha Shetty, Avila D’Souza, Sangeetha Poojari, Jayadurgi Narayana, Priya Rajeeva
      Pages: 375 - 381
      Abstract: Palm wine plays an important role as an alcoholic beverage in traditional practices. It is important to study the biochemical characteristics and microbiological aspects to understand the fermentation kinetics of palm saps. In the present investigation an elaborate study was carried out to study the fermentation kinetics of coconut palm sap. Total sugar, reducing sugar content and glucose concentration was estimated periodically during fermentation for 16h. Microbial load and invertase assay results were related to the changes in sugar concentration. Initial predominance of lactic acid bacteria was followed by dominance of yeasts. Hydrolysis of non reducing sugar occured at a faster rate between 3-9h of fermentation. During this period, the multiplication of yeasts began and reached its peak at 11h fermentation. Ethanol concentration was around 4.0 and 4.1%  at 11h and 13h of fermentation respectively.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 375-381
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18297
      Issue No: Vol. 5, No. 3 (2017)
  • Evaluation of Performances of Cowpea (Vigna ungiculata) Genotypes in
           Agronomy Farm of Lamjung Campus

    • Authors: Chandan Bhattarai, Dinesh Marasini, Prabin Dawadi, Sadkishya Aryal
      Pages: 382 - 385
      Abstract: Seed to seed production experiments for cowpea (Vigna ungiculata) was conducted at Agronomy farm at IAAS Lamjung, and to compare production potentialities of given cowpea genotypes under the given sets of conditions. The treatment consists of two cowpea varieties viz. Prakash (Standard Check) and Malepatan (Local check) and four genotypes; IT 99K-573-2-1, IT 86F-2062-5, IT 93K-452-1, IT 98K-205-8. The experiment was conducted on RCBD design. .All other activities were carried out according to prepared working calendar almost from August 2016 to December 2016.  Data collection were done for pre-determined growth and other yield parameters and was analyzed using MSTAT. Major parameters were Grain yield, Seeds/plant, Pods/plant, plant height, pod length, harvest index and test weight. Result shows maximum grain yield and harvest index was found for Prakash (Standard Check).Plant height and Pods /Plant was found highest for IT 99K-573-2-1. Similarly highest seed weight was found for Prakash (Standard Check) and Seeds/Plant and Pod length was maximum for IT 86F-2062-5.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 382-385
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18125
      Issue No: Vol. 5, No. 3 (2017)
  • Assessment of Antibiotic Residues in the Marketed Meat of Kailali and
           Kavre of Nepal

    • Authors: Rabin Raut, Ram Kumar Mandal, Krishna Kaphle, Dinesh Pant, Suresh Nepali, Arbindra Shrestha
      Pages: 386 - 389
      Abstract: This is a cross-sectional study to detect presence of antibiotic residues in meat sample of Kailali and Kavre districts. Each sample out of 55 meat samples (muscles and liver) of poultry-41, goat-12, buffalo-9 and pig-4 from Kailali and Kavre was tested for different groups of antibiotic residues separately. The antibiotics tested were Penicillin, Tetracycline, Aminoglycosides, Macrolides and Sulfonamides. Samples were preserved at -84˚C in the deep freezer after collecting in sterile plastic pouch. Rapid test kit (78.9% sensitivity and 96.7% specificity) of G9 Co. Ltd., Thailand was used to detect residues in the samples. The protocol of kit was followed for the test. The overall prevalence of antibiotic residues in meat was around 22%. Meat samples exceeding the Maximum Residue Limit (MRL) were 6. Liver was found with higher number of residues than muscles. The organ and location wise prevalence of residue was found statistically significant at p<0.05. The samples contained alarmingly high level of antibiotic residues which is because of neglecting the withdrawal period. The prescription of antibiotic is not prudent. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 386-389
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18302
      Issue No: Vol. 5, No. 3 (2017)
  • Bayesian Credible Intervals for Maize Grain Yields of the Maintenance
           Varieties Evaluated in Sudan

    • Authors: Siraj Osman Omer, Mohammed Salah Abdalla, Ibrahim Nuraldin Alzain, Abdelmoniea Dafaalla
      Pages: 390 - 396
      Abstract: Improved of maintenance crop varieties developed at agricultural research corporation, Wad median, Sudan is intended for seed foundation which is recently established. This study was undertaken to establish statistical investigation using Bayesian estimation for credible interval or posterior interval as a Bayesian strategy for a maintenance variety. Data on grain yield (kg/ha) on a maize crop variety were used. Bayesian posterior information can be annoying to investigate but are important in maintenance varieties that foundational claims are used to make general recommendations for practice. Half normal informative priors set were used. The heritability of yield (varieties)) was (h = 0.75). Predicted posterior means of varieties were shown with a Bayesian interval for scientific inference in the maintenance maize grain yield. Bayesian approach is useful for reducing uncertainty on decisions based on economic evaluation of new maize varieties in Sudan, the use of credible intervals for grain yield allow for early decisions.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 390-396
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18303
      Issue No: Vol. 5, No. 3 (2017)
  • Screening, Identification of Alkaline Proteases Producing Fungi from Soil
           of Different Habitats of Amalner Tahsil [Maharashtra] and Their

    • Authors: H.K. Suryawanshi, N.D. Pandya
      Pages: 397 - 402
      Abstract: Fungal proteases had wide applications in textile, leather, food and Pharmaceutical industries. As proteases shows proteolytic activity they are helpful in proteinic stain removal hence also used in various commercial detergent industries. For fungal isolation soil samples were collected from different sites of Amalner tahsil. (Maharashtra) e.g. crop fields, near dairy areas, poultry farms etc. Those soil samples showing alkaline pH were selected for isolation of fungi on Potato Dextrose Agar plates. Then among 14 different isolates 2 were selected for their most proteolytic activity after screening on casein agar, skimmed milk agar and gelatine agar. For submerged fermentation, these selected isolates were inoculated in production media in shake flask. After 72 hrs, plate assay was performed by taking crude enzyme after filtration and centrifugation as well as by taking partially purified enzyme.(partial purification done by ammonium sulphate precipitation method). Protease activity assay was performed by agar well diffusion method, as well as blood clot lysis activity and blood stain removal ability of protease obtained from selected isolates was studied.Selected isolates were identified,among them Aspergillus niger gives more proteolytic activity than Trichoderma longibrachiatum. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 397-402
      PubDate: 2017-09-27
      DOI: 10.3126/ijasbt.v5i3.18304
      Issue No: Vol. 5, No. 3 (2017)
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