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Indonesia Journal of Biomedical Science
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  This is an Open Access Journal Open Access journal
     ISSN (Print) 2085-4773 - ISSN (Online) 2302-2906
     Published by Udayana University Homepage  [1 journal]
  • PHOTOCHEMOPROTECTION OF CAULERA SPP ACTIVE COMPONENT ON RAT MODEL SKIN

    • Authors: A. A. G. P. Wiraguna, W. Pangkahila, and N. Mantik-Astawa
      Abstract: Caulerpa spp. including seaweed and macro-algae can be found in a large number at Coastal Serangan, Bali-Indonesia. Caulerpa spp was historically used for food and vegetable. However, in our today life, this plant was applied as material for skin care products, especially for UV protection. The aim of this study is to know the active component of caulerpa spp extract and its inhibition of premature skin aging induced by UV-B radiation through collagen expression. This was descriptive and experimental research applying a randomized posttest only control group design. Active component contained in the caulerpa spp extract are carotenoid, vitamin A, C, and E, polyphenols, minerals and amino acids. Topical application of caulerpa spp can suppress the UV-B radiation induced collagen damage. Increased of collagen expression in all groups of caulerpa spp. just as good as astaxanthin group in improving collagen expression and 0.2% of caulerpa spp. extract is the most effective dose in improving collagen expression.
      PubDate: 2013-07-06
      Issue No: Vol. 7 (2013)
       
  • AMPLICATION OF 0.7KB FRAGMENT KATG GENE FROM CLINICAL MULTI DRUG RESISTANT
           TUBERCULOSIS ISOLATE IN BALI

    • Authors: A. W. Dwiputri, K. Ratnayani, and S. C. Yowani
      Abstract: During last decade has seen a particular increase in the occurrence of drug-resistant of tuberculosis (DR-TB) and multi-DR strains, such as Isoniazid (INH) resistant strains of M. tuberculosis.  INH resistance is more frequently associated with mutations in the katG gene. Detection of katG gene mutations can be performed by PCR technique, followed by sequences. The aim of this study is to amplify katG gene region (0,7 Kb) from clinical isolate of MDR-TB in Bali. DNA isolation for PCR was done by Boom method and katG gene amplification was performed under the following conditions: predenaturation at 950C for 15 min; fourty cycles of denaturation at 940C for 1 min, annealing at 560C for 1 min, extension at 720C for 2 min; final extension at 720C for 10 min. The amplicons were detected by 1.5% agarose gel electrophoresis and showed a specific band size at 0.7 kb. This suggests that the fragment of katG gene has been successfully amplified in these areas.
      PubDate: 2013-07-05
      Issue No: Vol. 7 (2013)
       
  • MASSAGE STIMULATION REDUCES TUMOR NECROTIC FACTOR-ALPHA AND INTERLEUKIN-6
           IN PRETERM, LOW BIRTH WEIGHT WITH APPROPRIATE GESTATIONAL AGE NEWBORNS

    • Authors: I G. A. Trisna Windiani, S. Sotjiningsih, N. Mantik Astawa, and R. Kusnandi
      Abstract: Preterm newborn is susceptible from various stresses such as infections or non infections. During stress, immune response is activated by synthesizing and releasing of cytokines from activated immune cells into the circulation. One of the efforts to overcome the stress is massage stimulation. Several studies have been carried out to find out the benefit of massage stimulation. The objective of this study is to find out the effect of massage stimulation to reduce of TNF-' and IL-6 levels on preterm, low birth weight, appropriate to gestational age newborn. This was a randomized controlled trial. Subjects of the study were all preterm newborns with a stable medical condition, birth weight 1,500-2,499 gram, appropriate to gestational age. The study was carried out on October-December 2011, in neonatology ward, Sanglah General Hospital, Vali-Indonesia. The number of cases was 35 subjects and the number of controls was 36 subjects. The examination of TNF-', IL-6 levels and birth weights before and after massage stimulation were performed on both groups. The level of TNF-' and IL-6 was examined by applying ELISA method. The level of confidence was ' = 0.05. All statistical analysis was performed by computer programme. There was different level of cytokine pro-inflammation TNF-' (p=0.025) and IL-6 (p=0,001) comparing before and after condition massage stimulation. Outcome of body weight were also different at before and after massage stimulation, with p value 0.042. The conclusion of this study is that massage stimulation can reduce the level of TNF-',   IL-6 and increasing of birth weight on preterm, low birth weight appropriate gestational age newborn. Based on this study, in the future, massage stimulation can be done in newborn with stable medical conditions by parents or trained staffs.
      PubDate: 2013-07-03
      Issue No: Vol. 7 (2013)
       
  • ASSESSMENT OF HEALTH AND NUTRITIONAL STATUS UNDER THE INTEGRATED CHILD
           DEVELOPMENT SERVICES: in The Field Practice Area of Rural Training Health
           Centre, Nadayara, Travancore Medical College, Kollam-India

    • Authors: D. Sumit, and J. Abraham
      Abstract: Assessment of health and nutritional status was carried out in Anganwadi Children in the Field Practice Area of Rural Training Health Centre, Nadayara, Travancore Medical College, Kollam. This is a cross sectional study within community base setting. Data were collected by a pretested structured questionnaire and analyzed by applying SPSS. Undernourished children in the Anganwadis under study were 21 %. Growth pattern of these children were significantly associated with the birth weight and the episodes of diarrhea. A significant proportion of children are missing the booster doses. DPT booster (16-24 months) is only 74.1 % and DT booster (5-6 years) is only a meager of 13.6%
      PubDate: 2013-07-03
      Issue No: Vol. 7 (2013)
       
  • GARCINIA MANGOSTANA L. RIND EXTRACT AND PHYSICAL TRAINING REDUCE OXIDATIVE
           STRESS IN WISTAR RATS DURING MAXIMAL PHYSICAL ACTIVITY

    • Authors: I N. Arsana, N. Adiputra, J. A. Pangkahila, and I. B. Putra-Manuaba
      Abstract: Oxidative stress is a condition of imbalance between the production of free radicals or reactive oxygen species (ROS) and antioxidants, in which the levels of free radicals higher than antioxidants. One causes of oxidative stress is the maximum physical activity. Oxidative stress can be reduced by antioxidants. One source of antioxidants is mangosteen rind (Garcinia mangostana L). This study aimed to investigate the role of mangosteen rind extract and physical training in reducing Malondialdehyde (MDA, increasing Superoxide dismutase (SOD) and Glutathione Peroxidase (GPx). This study used a randomized block design with factorial pattern of 6 x 2 with four repetitions. The first treatments are mangosteen rind extract with doses; 0, 50, 100; 200; 300, and 400 mg/kg bodyweight/day for four weeks. The second treatments are the physical training consists of; without and with physical training. MDA, SOD and GPx were assessed in the end of treatment. Data were analyzed with GLZ. The results showed that average of MDA, SOD and GPx different significantly (p<0.05) after administration of extracts as well as physical training. Extracts and physical training concurrently also showed effect significant (p<0.05). However, at doses 0 until 300 mg/kg bodyweight, MDA levels more higher while SOD and GPx lower significantly (p<0.05) at training group compared with without training. Whereas at dose 400 mg/kg bw, MDA lower at training (p>0.05), while SOD and GPx were recorded significantly (p<0.05) higher than the group without training. Overall, it could be concluded that physical training with administration of mangosteen rind extract reduce oxidative stress through reduction of MDA, as well as increased both SOD and GPx.
      PubDate: 2013-06-05
      Issue No: Vol. 7 (2013)
       
  • ASSOCIATION OF B-CELL LYMPHOMA PROTEIN-2 AND CASPASE-3 EXPRESSION IN
           OVARIAN CANCER

    • Authors: I N. G. Budiana
      Abstract: Ovarian cancer remains a major problem of women’s health in the world, including Indonesia, and is associated with high rates of incidence and mortality. There are many efforts in early diagnosis on ovarian cancer, but until now there have not been found any satisfactory method. On the other hand, knowledge and research in the field of molecular biology become more advance, one of them is a mechanism to control the growth of cells in ovarian cancer through a process of programmed cell death or apoptosis. B-cell lymphoma protein 2 (Bcl-2) and caspase-3 are proteins that play a role on the mechanism of apoptosis. The purpose of this study was to determine the expression of Bcl-2 and caspase-3 and their association with ovarian cancer. Materials and method: The design of this study was a cross-sectional study. Expression of Bcl-2 and caspase-3 examined by immunohistochemistry under light microscope with 400x light power field and expression as a negative when the protein expressed in 10% or less of cells and as a positive when the protein expressed in more than 10% of cells. A number of 45 subjects were recruited in this study. Thirthy one of 45 subjects showed the expression of Bcl-2 positive (68.9%), while the positive expression of caspase-3 present in 20 subjects (44.4%). There was a significant association between the expression of Bcl-2 with the expression of caspase-3 in ovarian cancer patients (p=0.002; lambda=0.4). There was also a significant association between stage of disease with expression of Bcl-2 (p=0.002; lambda=0.3) dan expression of caspase-3 (p=0.001; lambda=0.3). Conclusion: It concluded that there is a significant association between the expression of Bcl-2 and the expression of caspase-3 in ovarian cancer.
      PubDate: 2013-06-05
      Issue No: Vol. 7 (2013)
       
  • HIGH INTERLEUKIN-6, LOW CD4+ AND CD8+ T-LYMPHOCYTES EXPRESSIONS AS RISK
           FACTORS OF CERVICAL CARSINOMA INFECTED BY HUMAN PAPILLOMA VIRUS TYPE-52

    • Authors: I G. A. S; Mahendra-Dewi, I K. Suwiyoga, E. Joewarini, and I G. Alit-Artha
      Abstract: In Indonesia cervical carcinoma is the most common cancer in women and one of the leading cause of mortality. High risk human papillomavirus (HPV) is the major risk factor of cervical cancer. This study aims to know the role of IL-6, CD4+ and CD8+ T-lymphocyte for the risk of cervical carcinoma infected by HPV52. This study was a case control study, specimens of cervical carcinoma patients infected by HPV type-52 as the case group and HPV type-16 or 18 as the control group. HPV genotyping used SPF10 primer and type specific E7 primer by LiPA. Immunohistochemistry method was used to know expression of IL-6, CD4+ and CD8+ T lymphocyte. Pearson’s c2 test was applied with statistical significance was set at the 2-sided 0.05 level. The odds ratios (OR) were calculated for the risk, with 95% confidence intervals on SPSS 16.0 for windows. PCR examination was performed in 185 paraffin-embedded tissue. The risk of high IL-6 expression in cervical carcinoma infected by HPV type-52 was statistically significant 6-fold higher compare with cervical carcinoma infected by HPV type 16 (OR = 6.00 ; CI 95% = 1.13-31.99; p = 0.03; p < 0.05) and HPV type 18 (OR = 6.00 ; CI 95% = 1.13-31.99; p = 0.03; p < 0.05). The risk of low CD4+ T lymphocyte expression in cervical carcinoma infected by HPV type 52 was statistically significant 6-fold higher and 7.43-fold higher respectively compare with cervical carcinoma infected by HPV type 16 (OR = 6.00 ; CI 95% = 1.003-35.91; p = 0.04; p < 0.05) and HPV type 18 (OR = 7.43 ; CI 95% = 1.23-45.01; p = 0.02; p < 0.05). The risk of low CD8+ T lymphocyte expression in cervical carcinoma infected by HPV type 52 was statistically significant 13.5-fold higher and 11-fold higher respectively compare with cervical carcinoma infected by HPV type 16 (OR = 13.50 ; CI 95% = 1.42-128.26; p = 0.01; p < 0.05) and HPV type 18 (OR = 11.00 ; CI 95% = 1.16-103.94; p = 0.02; p < 0.05). No significance different between cases and controls group in mean-age, parity and sexual activity (p > 0.05). In conclusion, this study found that high IL-6 expression, low CD4+ and CD8+ T lymphocyte expression were the risk factors of cervical carcinoma infected by HPV type 52.
      PubDate: 2013-06-04
      Issue No: Vol. 7 (2013)
       
 
 
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