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Journal Cover Anatomica Karnataka - an International Journal
  [1 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2229-7545 - ISSN (Online) 2249-5398
   Published by ScopeMed Homepage  [98 journals]
  • Physicochemical properties and plankton composition of the river Meghna,
           Bangladesh

    • Abstract: 2016-09-03T14-41-51Z
      Source: Asian Journal of Medical and Biological Research
      Md. Robiul Awal Hossain, Md. Mehedi Hasan Pramanik, Flura, Md. Istiaque Haidar and Yahia Mahmud.
      The present study was conducted to assess the physicochemical properties and plankton composition of water from the river Meghna (Shatnol to Chor Alexander) in thirteen (13) sampling spots during the period of July 2014 to June 2015. Ten (three were physical and seven were chemical) physicochemical parameters of water viz temperature (air and water), transparency, dissolved oxygen (DO), free carbon dioxide (CO2), Conductivity, pH, total hardness, total alkalinity and ammonia (NH3), plankton community of both phytoplankton and zooplanktons were studied in aforesaid 13 sampling spots of the river Meghna. Maximum air temperature was recorded 37°C at Ananda Bazar. Maximum water temperature was recorded 30°C at Kaligonj, Chor Ludhua and Chor Alexander area of the river Meghna. Transparency was found minimum 12 cm at Chor Ludhua and maximum 75 cm at Horina Ghat. The concentration of dissolved oxygen was found maximum at Chor Voirabi and Horina Ghat 7.3 mg/l and was found minimum at Kaligonj 3.5 mg/l. Free CO2 was found highest at Kaligonj 17 mg/l and lowest at Horina Ghat 5 mg/l. pH was found ranged from almost neutral to alkaline (Minimum 7.2 to Maximum 9). Total hardness was found highest at Chor Alexander 802 mg/l and lowest at Eklaspur 35 mg/l. Total alkalinity was found highest at Chor Alexander 145 mg/l and found minimum at Madrasa Ghat and Hizla 29 mg/l. Conductivity was found highest at Chor Alexander and Chor Ludhua 1000 μs/cm. Ammonia concentration was found ranged from 0.00 to 0.03mg/l. The mean contribution of phytoplankton was about 90.5% of the total planktonic organisms and zooplankton contributed the rest. The major groups of phytoplankton were found Bacillariophyceae, Chlorophyceae, Cyanophyceae, Dinophyceae, Euglenophyceae, Myxophyceae and Xanthophyceae. Total 9 genera of zooplankton were identified from four families namely Rotifera with 2 genera, Cladocera with 3 genera, Copepoda with 3 genera and Ostracoda with 1 genus from the selected sampling spots.

      PubDate: Tue, 30 Aug 2016 04:54:46 GMT
       
  • Distribution of sul genes and their variants in uropathogenic Escherichia
           coli isolated from two hospitals of Sabah

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Zaw Lin, Yun Mei Lai, Myo Thura Zaw and Ahmad Toha Samsudin.
      Sulphonamides resistant strains are highly prevalent in uropathogenic Escherichia coli (UPEC) isolates. Sul genes encode sulphonamide resistance and are present on transferrable plasmids. Integrons (IGNs) are genetic elements containing integrase gene, attl site and gene cassettes which carry multiple antibiotic resistant genes. Class 1 integrons have been extensively studied because these were most prevalent among clinical isolates. In this study, UPEC isolates were determined for the antibiotic susceptibility patterns to four antibiotics commonly used for urinary tract infections, which include co-trimethoxazole (TMP-STX). Distribution of sul genes and integrase1 gene (intI1) was studied in TMP-STX resistant UPEC isolates by using multiplex polymerase chain reaction (mPCR). Sul genes variants were investigated by DNA sequencing of the whole open reading frame of sul1 and sul2 genes and PCR product of sul3 gene. Sul1, sul2 and sul3 genes were prevalent in 37 (24.7%) of 150 UPEC isolates. IntI1 is positive in 22 sul genes positive isolates. Of six isolates positive with sul2 genes, sul2(a) and sul2(b) variants, which were described in the previous study, in the four isolates and the two isolates respectively were observed. This is the first mPCR which investigates the prevalence of three sul genes and intI1 in the UPEC clinical isolates from two hospitals of Sabah.

      PubDate: Mon, 08 Aug 2016 08:26:40 GMT
       
  • Effect of milk replacer on kid performance among small holder farmers

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      M.Z. Rahman, M.A.I. Talukder, Md.Yousuf Ali and Most. Sumona Akter.
      An experiment was conducted with 15 Black Bengal kids of both sexes (in 3 groups) were fed two different milk replacer using ingredient shotti (T1), skim milk (T2) and no milk replacer- kids with mother, termed as control group (T0) at Southern agro trade, Subarnachar, Noakhali. The average total DM intake and milk dry matter intake were not significantly differed among the treatment group. Total dry matter intake was not affected by intake of liquid milk replacer or goat milk. The CP intake were ranged from 19.97 to 20.93 g/d. Total CP intake was not significantly differed among the group but T1 group was slightly higher(0.42 g/d) than T0 and T2. The amount of protein intake (g/d) was followed NRC (1985) recommendation. DMI from concentrate (g/d), DMI from green grass (g/d) and DMI from percent live weight were not significantly differed among the treatment groups. The average daily gain was not significantly (P>0.05) differed among the treatment group. The average growth rate (57.42 – 61.20 g/d) of all kid of Black Begal goat was lower than the Angora goats (115.00-125.00 g/d) and Alpine goats (153.00-258.00 g/d). Dry matter (DM) digestibility (%) was significantly (p

      PubDate: Sun, 07 Aug 2016 09:17:54 GMT
       
  • Prevalence and pathology of Newcastle disease in broiler at Bochaganj
           Upazila of Dinajpur, Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Pabitra Kumar, S. M. Harun-ur-Rashid, Md. Haydar Ali, Hosne Mobarak, Md. Aminul Islam and Rifat Haydar.
      The study was schemed to investigate the prevalence, pathological conditions, mortality and clinical features of Newcastle disease in the small scale commercial broiler farms at Bochaganj upazila of Dinajpur district during January to June, 2014. A total 1950 birds (from 5 farms), among which 160 diseased and dead birds were selected out of which 99 (5.35%) birds were found to be positive for Newcastle disease. The clinical signs of the affected birds were sneezing, coughing, nasal discharge, laboured breathing, and torticolis. Broiler were inactive, weak and rough in appearance, greenish watery diarrhoea occur severely. Nervous sign include clonic, spasm and paralysis of the legs and wings. In this observation, the gross pathological lesions were slight to severe haemorrhages in caecal tonsils, typical lesions were proventricular haemorrhage, most commonly seen in the surface near the junction with the proventriculus. The prevalence of Newcastle disease in Bochaganj upazila was 5.35%. Mortality of Newcastle disease in non-vaccinated and vaccinated broiler flock was 20.76% and 4.6%, respectively.

      PubDate: Sun, 07 Aug 2016 09:15:08 GMT
       
  • Comparative performances of hilly chicken and naked neck hilly chicken at
           Naikhongchari hilly areas of Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Azharul Islam Talukder, Md. Ashadul Alam, Md. Moklesur Rahman, Md. Abu Hemayet and Md. Asadul Islam.
      A total of 115 hilly chickens (92 female and 23 males) and 35 naked neck hilly Chickens (28 females and 7 males) were reared in open sided poultry house for 10 months period to compare their productive and reproductive performances. The mean body weights of adult female and male hilly birds and naked neck hilly birds were 2244, 2005 and 2664, 2576 g respectively. The age at first egg of both type of birds were 147 and 159 days respectively and hen-day egg production were 27±1.4 and 35±3.3 respectively. The average egg weight was 41±4 and 38±3.6 g respectively. Both type of bird per day average feed consumption were 97.61±11 and 102±22 g and mortality were 15.56±3.7 and 11.42±2.7 percent respectively. The average hatchability percentage performed by 14 broody hens reared on litter floor was 67.5±10 and 57±22 percent respectively. The average hatching egg weight and chick weight were 47.28±3, 41.48±1 and 31.4±3, 29.6±4 g respectively. The body weight of growing hilly bird and Naked Neck hilly bird after eleven weeks of age were 727.3±17.4 and 645±97.6g, respectively and Feed conversion ratios (FCR) were 3.39 and 3.34 respectively. It was concluded that Hilly chicken improved by body weight than previous year and both feathered and Naked Neck Hilly chicken given an indication of meat type native chicken and required to reproduce to have large stock for undertaking conservation and further improvement programme.

      PubDate: Sun, 07 Aug 2016 09:12:03 GMT
       
  • A study on consumption of iodine by an individual in selected area in
           Tangail, Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Sabina Yeasmin, Najia Kamrul, M. Burhan Uddin and Md. Fahad Jubayer.
      The purpose of this study was to assess consumption of iodized salt by an individual (g/person/day) in a village of Tangail district and to compute the intake of iodine by an individual (µg/person/day) in a village in Tangail district. Vacuum evaporated salt was used for the study. The salt was analyzed for chemical composition and iodine content. Various physico-chemical characteristics of salt were analyzed to determine the moisture content, chloride content (as NaCl), water insoluble matter, pH, calcium content, magnesium content, and iodine content. Study was done among 68 families for a period of 35 days. Family member was divided into 4 groups according to their age – below 5 years, 5-9 years, 10-19 years and above 19 years. Male member among the family members was minimum 1 and maximum was 8. In case of female, minimum number was 1 and maximum number was 7. Among family members, 45.58% were male and 54.42% were female consumers. Percentage of salt consumers among Family members below 5 years was 11.81%, in 5-9 years it was 6.94%. Percentage of salt consumer in family members between 10-19 years was 24.54%, and salt consumers percentage in family members above 19 years was 56.71%. By statistical analysis among data, mean value of salt consumption g/ person /day was 12.49, standard deviation was 2.05 and minimum salt consumption among family members was 10.02 g/person/day and maximum value was 18.47 g/person/day. Among the family members, mean value of intake of iodine was 399.68 µg/person/day, maximum intake of iodine was 591.04 µg/person/day and minimum intake was 320.64 µg/person/day. Finding of this study was that, salt consumption rate are satisfactory and intake of iodine by individual in village Chamuria in Tangail district is adequate to avoid Iodine Deficiency Disorder in normal health condition.

      PubDate: Sun, 07 Aug 2016 09:09:07 GMT
       
  • Isolation, molecular identification and antibiotic susceptibility profile
           of Aeromonas hydrophila from cultured indigenous Koi (Anabus testudineus)
           of Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Shuvho Chakra Borty, Fabaya Rahman, A.K.M. Ali Reza, Mst. Sharifa Khatun, Md. Luthful Kabir, Muhammed Hafizur Rahman and Md. Shirajum Monir.
      Fish play a crucial role in the Bangladeshi diet, providing more than 60% of animal source food, representing a crucial source of micro-nutrients and possessing an extremely strong cultural attachment. In this study isolation and identification of Aeromionas hydrophila was done by studying cultural properties, Gram’s staining and biochemical properties of isolates of diseased indigenous Koi fish (Anabas testudineus) of different upazillas of Mymensingh district. Antibiogram profile of the isolated bacteria was studied by using wide range of commercially available antibiotics. Quantitative study of bacteria isolated from diseased indigenous Koi fish showed variation of number in different organ. Total bacterial load was found to be 1.90 × 105, 1.19 × 105, 3.21 × 105, 2.18 × 106 and 3.14 × 105 cfu/g in lesions; 2.52 × 107, 2.34 × 108, 5.41 × 108, 2.54 × 109 and 5.21 × 109 cfu/g in liver; 2.54 × 108, 2.41 × 108, 1.90 × 107, 3.65 × 107 and 3.45 × 108 cfu/g in spleen; 3.51 × 107, 5.28 × 107, 3.14 × 106, 1.85 × 107 and 4.52× 107 cfu/g in kidney in diseased Koi of Mymensingh sadar, Muktagacha, Tarakanda, Gouripur and Fulpur upazillas, respectively under Mymensingh districts. Aeromonas hydrophila was initially identified by their specific morphological, physiological and biochemical characteristics. Then molecular detection of A. hydrophila was done by PCR. PCR products of desired 760 bp were obtained for A. hydrophila. The results of the antibiotic sensitivity test is exhibited that most of the bacterial samples were sensitive against ciprofloxacin (92%) and levofloxacin (84%), intermediate resistant against gentamicin (40%) and resistant against novobiocin (84%), ampicillin (100%) and penicillin (92%).

      PubDate: Sun, 07 Aug 2016 09:05:50 GMT
       
  • Effect of seed washing either alone or in combination with garlic extract
           and Knowin 50WP on quality of jute seeds

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      M. S. Islam and M. S. Monjil.
      Efficacy of seed washing, garlic extract, prewashed seeds treated with garlic extract, Knowin 50 WP (Carbendazim) and prewashed seeds treated with Knowin 50WP were evaluated on seed borne fungi of jute seeds. Nine seed borne fungi were detected on both deshi and tossa jute seed samples collected from sadar upazilla of Barisal district viz., Macrophomina phaseolina, Botryodiplodia theobromae, Fusarium spp, Colletotrichum corchori, Curvularia lunata, Aspergillus flavus, A. niger, Penicillium spp. and Alternaria spp.. Physical seed washing and garlic extract were found effective against seed borne diseases of jute which significantly decrease the association of seed borne fungi from jute seeds. Prewashed seeds treated with garlic extract showed highest germination and highest reduction of seed borne mycoflora from desi and tossa jute seeds. Knowin 50WP or prewashed seeds treated with Knowin 50WP were found effective to reduce seed borne fungi from jute seeds. Highest vigor index was obtained in jute seedlings raised from prewashed seeds treated with Garlic or Knowin 50WP. Thus, prewashed seeds treated with Garlic or Knowin 50WP resulting significant reduction of seed-borne fungal population and enhancing seeding vigor.

      PubDate: Sun, 07 Aug 2016 08:59:27 GMT
       
  • Potentiality of producing summer cauliflower as influenced by organic
           manures and spacing

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Laila Farzana, Abul Hasnat M Solaiman and Md. Ruhul Amin.
      The experiment was conducted to find out the effect of organic manures and spacing on the growth and yield of cauliflower in summer season. In this study, the treatment consisted of three organic manures viz. F0: no organic manure, F1: cowdung, F2: vermicompost and three spacing viz. S1 (60 × 30) cm, S2 (60 × 40) cm, S3 (60 × 50) cm. Two factorial experiments were laid out in the RCBD with three replications. Significant variations in all parameter were observed due to organic manure and spacing at different days after transplanting. For organic manure, highest yield of cauliflower (12.98 t ha-1) was obtained from F2 and lowest (8.24 t ha-1) from F0. For spacing, highest yield of cauliflower (11.25 t ha-1) was obtained from S1 and lowest (10.57 t ha-1) from S3. For combined effect, highest yield of cauliflower (13.33 t ha-1) was obtained from F2S1 and the lowest (7.91 t ha-1) from F0S3. The highest BCR (3.79) was found from F2S1 and lowest (2.7) from F0S3. It is found from the experiment that growth and yield of summer cauliflower were positively correlated with organic manure and spacing. However, white beauty cultivars can be cultivated in summer season and use of vermicompost with 60×50 cm spacing would be beneficial for the farmers.

      PubDate: Sun, 07 Aug 2016 08:56:14 GMT
       
  • The activities of Bangladesh water development board on the social
           environment of Bhadrabila union, Narail sadar upazila, Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Rimi Bhattacharjee and Md. Amit Hasan.
      The study was conducted at Bhadrabila union, Narail sadar upazila, Narail in Bangladesh. The study period was carried out during September to December, 2013. In the selected areas, the study was conducted on 375 persons. The collected information was totally interview and observation based study. In the study area the highest age distribution was 50% in 21-40 years. The second highest was 39% for 41-60 years. The third highest was 7% for the year of 1-20. The lowest age distribution was for the year of 60 and above. This study found that the highest respondents of 39% are the member of 3-4 family member range. 32% respondents are in the member of 5-6 family member range. 12% respondents are in the member of 7-8 family member range. This study represent that 18% houses were kacha, 27% houses were tin shade 29% half building and 26% house were building. Almost 3% respondents were in class 1-3, 31% were in class 4-6, 46% were in between class 7-9, 10% in S.S.C level and left 9% were in above S.S.C level. Almost 79% had sanitary latrine. . It was found that 28% peoples were and poor their monthly income was 3,000-6,000 taka, 40% peoples were middle class their monthly income was 6,000-9,000 taka, about 24% peoples were rich their monthly income was 9,000-12,000 and lest 8% peoples income were 12,000+. 90% tube wells were arsenic free and only 10% tube wells are arsenic contaminated. About 77% were related with the BWDB and rests 13% were not the beneficiary of the BWDB. About 86% respondants were benifited by the activities of the BWDB. About 100% of respondents are fully satisfied by the activities of BWDB in arsenic test. It was shown that the social life of the respondents of Bhadrabila Union Parisad was getting better. The most important thing is almost 100% of the respondents were satisfied by the activities of BWDB.

      PubDate: Sun, 07 Aug 2016 08:52:29 GMT
       
  • Excavation of irrigation canal changes the cropping pattern in 2013-14:
           Auria Union, Narail, Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Amit Hasan, Md. Shamsur Rahman, Md. Atiqur Rahman, Shaswata Biswas, Rimi Bhattacharjee and Md. Maruf Shaikah.
      The principal crop during winter is Bororice and it occupies about 70% of crops. Being a country of 140 million inhabitants, agriculture is still the major water using sector in order to feed a growing population where at least 40 million people do not have a square meal. Recently, Bangladesh Water Development Board (BWDB) launched some projects to various water user groups for maintenance and expenditure recovery. Chenchuri Irrigation Sub Project is under South-West Area Integrated Water Resources Planning and Management Project (SWAIWRPMP). The project area covers the South Western Narail District along with Auria Union at Narail Sadar Upazila. Data were collected from 150 farmers of 6 villages in Auria Union. This research investigates the overall cropping pattern in Auria Union, Narail Sadar Upazila, before the construction of canal (during 1987-88), after the construction of canal (during 2009-10)and after re-excavation of canal (2012-13). The result showed that, before the construction of canal (during 1987-88) there were 5 types of cropping pattern and after the construction of canal (during 2009-10) there were 6 types of cropping pattern. But after re-excavation of canal (2012-13), 12 types of cropping pattern were recorded. Among them, the T. Aman and Boro were the most prominent cropping pattern. In Kharif 1, Kharif 2 and Rabi seasons, various types of crops were cultivated. Such as Aus, B. Aman, T. Aman, Boro, Jute, Sesame, Summer vegetables, Chilly, Garlic, Chickpea, Lentil, Mustard, Onion, Sugarcane, Turmeric, Wheat, Winter vegetables etc. The result revealed that, the re-excavation of canal have significantly positive impact on the Boro, Wheat, Turmeric, Chilly, Chick pea, Sugarcane, Lentil production. The Boro rice cultivation increased significantly day by day and it replaced other crops gradually. Because, farmers get irrigation water easily and cheaply from canal in Rabi season (16 October – 15 March). The present study showed that, the excavation of irrigation canal completely changes the cropping pattern at auriaunion in Narail, Bangladesh.

      PubDate: Sun, 07 Aug 2016 08:48:49 GMT
       
  • Deep placement of N fertilizers influences N use efficiency and yield of
           BRRI dhan29 under flooded condition

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Samia Lutfa Hasan, Md. Rafiqul Islam, Mahmud Hossain Sumon and Azmul Huda.
      An experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during boro season of 2014 to evaluate the effect of deep placement of nitrogen (N) fertilizers on N use efficiency and yield of BRRI dhan29 under flooded condition. The soil was silt loam in texture having pH 6.27, organic matter content 1.95%, total N 0.136%, available P 3.16 ppm, exchangeable K 0.095 me%, available S 10.5 ppm and EC 348 μS cm-1. The experiment was laid out in a Randomized Complete Block Design (RCBD) with eight treatments and three replications. The treatments were T1 [Control], T2 [Prilled urea (130 kg N ha-1)], T3 [USG, 130 kg N ha-1], T4 [USG, 104 kg N ha-1], T5 [USG, 78 kg N ha-1], T6 [NPK briquette, 129 kg N ha-1], T7 [NPK briquette, 102 kg N ha-1] and T8 [NPK briquette, 78 kg N ha-1]. All the treatments except T6, T7 and T8 received 25 kg P and 64 kg K ha-1 as TSP and MoP respectively. In T6, T7 and T8 treatments, P and K were supplied from NPK briquettes. Prilled urea was applied in three equal splits. USG and NPK briquettes were applied at 10 DAT and were placed at 8-10 cm depth within four hills at alternate row. After deep placement of USG and NPK briquette, and each split application of PU, the water samples were collected for 5 consecutive days and analyzed for NH4-N concentration in surface water. After application of N fertilizers, the NH4-N in floodwater reached peak on the 2nd day in PU treated plots and then decreased rapidly over time. In the USG and NPK briquette treated plots, the NH4-N was generated slowly but spontaneously over the entire growth period as compared to PU indicating a beneficial role of USG and NPK briquette. The highest grain yield of 7.16 t ha-1 was recorded for T6 [NPK briquette, 129 kg N ha-1] which was statistically similar to that of T3 [USG, 130 kg N ha-1] and T4 [USG, 104 kg N ha-1]. The highest straw yield of 8.05 t ha-1 was obtained for T3 [USG, 130 kg N ha-1]. The deep placement of USG and NPK briquettes enhanced the recovery of applied N and N use efficiency in comparison with the broadcast application of N fertilizers.

      PubDate: Sun, 07 Aug 2016 08:45:11 GMT
       
  • Study on effect of different levels of concentrate on growth performance
           of both male and female lamb

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Masum Billah, Md. Humayun Kabir, Md. Hafizur Rahman and Md. Abdul Hamid.
      The present study was carried out to assess the effect of concentrate on growth performances of both male and female lamb. For this purpose twenty four (12 male and 12 female) lamb aged about 5-6 months were selected and divided into three groups (Treatment group T1, T2 and control group T0). In each group having 4 males and 4 females lamb, all lambs were supplied green roughages ad-libitum, control group T0 was allowed no concentrate and treatment group T1 & T2 were supplied 100 gm & 200 gm concentrate mixture respectively for 90 days experimental period. Animals were weighed at 15 days interval. Significant differences of live weight gain among these three groups were found. Allowing 100 gm (T1) concentrate mixture along with green grass improved growth rate in both male and female lamb. Increasing of concentrate supplementation improved live weight gain (found in T2 group). Total live weight gain (kg) and average daily live weight gain (g/d) were 4.25±0.52 kg and 47.20±5.75 g, 5.38±0.83 kg and 59.73+9.17 g and 7.00±0.54 kg and 77.78±6.00 g in male lambs and 2.25±0.21 kg and 25.00±2.34 g, 3.18±0.32 kg and 35.39±3.63 g and 3.81±0.24 kg and 42.33±2.77 g in female lambs for the To, T1 and T2 groups respectively. Live weight was significantly (P

      PubDate: Sun, 07 Aug 2016 08:42:03 GMT
       
  • Uses of milk in sweetmeat shops and consumer preferences to milk products
           at Mymensingh municipality in Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      A.B.M. Kawsar Ahmed, Md. Rezwanul Habib, Samia Afrin, Mohammad Ashiqul Islam and Md. Harun-ur-Rashid.
      The work has been designed to investigate the utilization of milk for consumption of fluid milk and milk products, their distribution pattern, pricing, shelf life and consumer’s preference of Mymensingh municipality in Bangladesh. The study was based on milk and milk products and data were collected from the selected sweetmeat shops by direct interview, of which 20 samples were collected from sweetmeat shops and 7 from goalas. Both tabular and statistical methods were used for collected data analysis. Shopkeepers of different sweetmeat shops received raw milk from farmers (52.6%) and goalas (47.4%) and the highest amount of whole milk was required in per unit production of rasomalai (21%) and ghee (18%) whereas the lowest amount in chomchom (9%). Milk products prices were not remained same throughout the year in this municipality due to fluctuation of raw milk availability and their price. Eid, Puja festivals and other educational activities results that may increase milk products selling especially rasogolla and kalojam. Research findings also showed nonsignificant difference in case of pricing, distribution pattern, shelf life and selling of milk and milk products following sweetmeats.

      PubDate: Sun, 07 Aug 2016 08:38:56 GMT
       
  • Evaluation of growth performance of Brahman cross calves to local
           environment of Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md Azizul Haque, Mst. Fatematuzzohora, Md. Azharul Hoque and Md. Younus Ali.
      The present study was conducted using growth performance data on 624 Brahman cross (25%) calves collected from three villages adjacent to Bangladesh Agricultural University, Mymensingh, Bangladesh. Growth performance data of 289 Brahman cross (50%) calves were also collected from record sheet maintained at 12 Upazila Livestock Offices under the “Beef breed development project” of Department of Livestock Services to compare performance between 25% and 50% Brahman cross calves. Growth performance traits were considered birth weight, weight at three-, six-, nine-, twelve-month of age, average daily gain from birth to twelve-month of age. Calving difficulties (dystocia) and calf mortality were also included in the study area. The birth weight, weight at three-, six-, nine- and twelve-month average daily gain for 25% Brahman cross calves were 19.79±0.20, 52.72±1.31, 86.61±2.02, 129.90±3.08, 172.60±3.48 kg and 426.00±4.99g, respectively. The average birth weight (21.40±0.24 kg) and twelve-month weight (229.62±2.08 kg) of 50% Brahman cross calves were significantly higher (p

      PubDate: Sun, 07 Aug 2016 08:35:59 GMT
       
  • Determination of appropriate level of fat in milk for the production of
           good quality dahi

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Morsheda Yesmin, Md. Rezaul Hai Rakib, Al Amin Hossain, Akhi Khan and Md. Nurul Islam.
      The research was conducted to determine the approximate fat in milk for good quality dahi production and to inform dahi manufacturers’ about the acceptable level of milk fat to be used and also their economic benefit. Quality assessment tests were conducted on four different types of dahi prepared by adding different percentage of milk fat such as 3.5% (A), 4.0% (B), 4.5% (C) and 5.0% (D) respectively. All the samples were analyzed for organoleptic, chemical and microbiological qualities. The scores for smell and taste, body and texture, color and appearance, and total scores for four types of dahi samples showed significant differences at various levels (p

      PubDate: Sun, 07 Aug 2016 08:32:42 GMT
       
  • Effect of fortification of skim milk with coconut milk on the proximate
           composition and manufacture of dahi, a traditional sweet curd

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Rezaul Hai Rakib, Morsheda Yesmin, Md. Abu Hemayet, Md. Ahsanul Kabir and Md. Nurul Islam.
      The experiment was conducted to measure the feasibility of partial replacement of skim milk with different levels of coconut milk in the manufacture of dahi. Skimmed milk was replaced by 0, 5, 10, 15 and 20% of coconut milk to produce Dahi designated as A, B, C, D and E. All the samples were analyzed for organoleptic, chemical and microbiological qualities. Replacement of skim milk up to 10% with coconut milk increased total organoleptic score but score decreased when level of coconut milk was 15% and 20%. Dahi manufactured by incorporating 5% coconut milk gave superior results for body and consistency and also for colour and texture. In all levels of replacement increased fat, carbohydrates, ash and total solids were obtained, while protein and pH content were significantly decreased in Dahi samples. Total bacterial count was higher in the dahi manufactured by replacement of coconut milk than control. It could be concluded that 5 and 10% replacement of skim milk with coconut milk for the manufacturing of Dahi was acceptable and reduced the production cost and they were better in compare to others, according to organoleptic, chemical and microbiological analysis. The work showed the potential of coconut as an alternative source of skim milk in dahi manufacturing with improved nutritional value and consumer acceptability.

      PubDate: Sun, 07 Aug 2016 08:27:55 GMT
       
  • Molecular characterisation of oomycetes from fish farm located in
           Mymensingh sadar during summer

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      K. M. Mahfujur Rahman and Mohammad Nasif Sarowar.
      Oomycetes, also known as water molds, can cause serious infection to plants and animals especially at lower temperature although they dwell in freshwater and moist ecosystem throughout the year. The aim of this research was to investigate the diversity of oomycetes inhabiting in small water bodies during summer. Three types of samples i.e. water, fish mucus and apparently infected muscle samples of fish were collected from a large fish farm consisting of over 100 medium to large ponds in Mymensingh during summer (March to June) in 2015. A total number of 385 samples (284 of water, 79 of mucus and 22 of apparently infected muscle samples) were collected in 15 ml sterile falcon tubes with baits in each. Eleven of the isolates were isolated in Potato Dextrose Agar (PDA) plates and were identified using molecular methods that included DNA extraction, PCR amplification and subsequent sequencing of the ITS region of the genomic DNA of the samples. BLAST analysis to GenBank revealed that two of the isolates were 99% similar to Pythium sp. (HQ643814), three of the isolates were 98-99% similar to Pythium sp. (KT247392), and each of the remaining four isolates was similar up to 99% to Pythium sp. (KF836354), 99% to Pythium sp. (EU544193), 99% to Pythium rhizo-oryzae (HQ643757) and 100% to Pythium catenulatum (KP862946). Two of the eleven isolates were not assessed due to sequencing error. Phylogenetic analysis revealed that six of the isolates are of clade B1 and three of the isolates are of clade B2 in the Pythium phylogeny. The results partially suggest that plant pathogenic oomycetes are more common in summer than animal or fish pathogenic isolates in the sampled farm however; intensive sampling with a broad range of freshwater ecosystems during summer can give a clearer view on oomycete diversity in Bangladesh.

      PubDate: Sun, 07 Aug 2016 08:21:35 GMT
       
  • Gross and histomorphological effects of formaldehyde on brain and lungs of
           Swiss albino mice

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Marya Afrin, Tanjina Amin, Md. Rabiul Karim and M. Rafiqul Islam.
      Formaldehyde (FA) is toxic over a range of doses and of particular concern to anatomists and medical students on laboratory use. FA induces several characteristics of neurotoxicity in addition to systemic effects. Therefore, to know the toxic effects of FA on brain and lungs, mice were collected from international center for diarrheal disease research. They were divided into four groups i.e. control, inhalation, oral and intraperitoneal. The inhalation, oral and intraperitoneal exposure groups further divided into three subgroups which were subjected to exposure of FA daily for 30 days in case of inhalation and oral groups and 10 days in intraperitoneal group for acute toxic effects. Morphological study showed gross abnormalities i.e. congestion on lungs. But no gross abnormal features observed on brain. In 5 ppm treated inhaled group, lungs tissue revealed hemorrhages. In the inhaled groups, brain tissue showed degenerating neurons with either pyknotic or karyorrhectic nuclei are gradually increased in highest concentration group. These data supports the view that FA has adverse effects on the morphology of brain and lungs.

      PubDate: Sun, 07 Aug 2016 08:16:09 GMT
       
  • Growth response of Aspergillus flavus IMS1103 isolated from poultry feed

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Monzur Morshed Ahmed, Md. Fakruddin, Md. Nur Hossain, Khandaker Rayhan Mahbub and Abhijit Chowdhury.
      Aspergillus flavus strains were isolated from locally available poultry feeds. Effect of temperature, pH and culture media on growth of Aspergillus flavus was studied. Temperature ranged from 4-42°C (4, 10, 20, 25, 30, 37 and 42°C) was examined. Except for 4°C and 10°C, the isolate was able to grow for the whole temperature range. The growth was maximum at 25°C and was influenced with increasing or decreasing of temperature from 42°C to 20°C.The lag time was strongly influenced by the temperature at lower temperature level than at higher temperature range. Effect of pH on growth of Aspergillus flavus was also examined; from comparison of 3 different pH levels, it is concluded that at most temperatures pH 6.5 showed a higher growth rate and as a consequence required a shorter time to achieve maximum colony diameter. No significant variations in the lag time were observed. A natural poultry feed meal agar medium (FMAM) was developed in the laboratory and growth of A. flavus was compared with other 2 synthetic dehydrated media namely; Czapek’sdox Agar (CDA) and potato dextrose Agar (PDA). Poultry feed meal agar medium showed better growth response than Czapek’sdox agar and potato dextrose agar at all conditions. At 25°C and pH 6.5 found optimum for growth of Aspergillus flavus in feed meal agar medium whereas, temperature 30°C and pH 6.5 found optimum for growth for Czapek’sdox agar media and temperature 30°C and pH 6 showed high growth rate on potato dextrose agar. Poultry feed meal media showed high affinity for growth of mycelium and early spore formation than other media examined.

      PubDate: Sun, 07 Aug 2016 08:12:44 GMT
       
  • Present status, problem and prospect of duck farming in rural areas of
           Mymensingh district, Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Asadul Islam, Md. Abdur Rahman Howlider, Md. Ashadul Alam, Md Abu Heyamet and Manika Debnath.
      The study was conducted to know the present status, existing production system of duck and assess the potentiality of duck rearing in rural areas of Mymensingh district in Bangladesh. Data were collected randomly from 50 duck rearing farmers using a pre-tested interview schedule during March to May 2010 from several villages under sadar upazilla of Mymensingh. The results reveal that most of the farmers (60%) were middle aged. About 32% farmers were illiterate. Most of the farmers (52%) reared deshi duck and duck population per household was 11.1. About 80% wife of farmer’s household were responsible for duck rearing. All farmers reared duck in semi scavenging system. About 36% farmers used wood and tin for construction of duck house and 94% farmers used bedding materials for their duck house. All farmers used rice in the diet for duck and 62% farmers accumulated rice and rice polish to make diet for their ducks. About 40% farmers provided on an average of 121.91g supplemental diet to each duck/day and cost of the diet was Tk. 0.85/duck/day. Most of the farmers (72%) provided diet to their ducks twice a day. The age and weight of duck at maturity were 183.6 days and 1.69 kg, respectively. Egg production/duck/year and weight of each egg were 117.5 no’s and 63.8g, respectively. All farmers incubated duck egg under broody hen and they got 85.83% hatchability on set eggs. Most of the farmers (52%) mentioned that most prevalent disease of duck was cholera and their duck mortality was 15.2%. About 52% farmers controlled their duck disease with medication and only 14% farmers used vaccine to prevent duck disease. About 22% and 13.5% people did not consume duck meat and egg, respectively because of odour, asthama and allergy. Most of the farmers (50%) incubating duck eggs for ducklings. About 66% farmers purchased duckling by Tk. 24-25 and 58% farmers sell adult duck by Tk. 175-190. Most of the farmers (81.25%) stated that the duck farming is decreasing day by day. About farmers (51%) stated that reason of decreasing duck farming was lack of scavenging area. It was concluded that duck rearing knowledge of the farmers such as breeding, feeding, housing, prevention and control of diseases are not satisfactory of this areas. Introducing of improved duck breeds/varieties, training to duck farmers, ensuring vaccination to ducks, financial and technical support to the farmers could increase the duck rearing with increased household income and employment to youth, rural women and the small-holder marginal farmers.

      PubDate: Sun, 07 Aug 2016 02:55:29 GMT
       
  • Factor shares analysis of livestock products in selected areas of
           Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Mamun Or Rashid, M. Kamruzzaman and Md. Kaosar Niaz Bin Sufian.
      A study was undertaken to assess the contribution of different inputs such as feed, labor veterinary services and depreciation cost etc. to the development of livestock sector including poultry in the study area. The factor shares of livestock products was estimated based on the primary data for the periods of April to July, 2012.The factor shares of livestock production suggested that current factors (Feed, hired labor, veterinary services and other inputs) and family labor was the major component in the livestock production. Traditional milk producing farmers in Gazipur were not benefited from their farming due to residual share was negative (11.15%). For commercial milk production, on an average the largest share current factors (60.54 percent) followed by residual (28.1 percent), family labor (6.63 percent), depreciation (4.74 percent), respectively and residual share was positive. Production of beef cattle on an average the largest share current factors (74.26 percent) followed by family labor (21.73 percent), depreciation (46.425 percent), respectively and residual share was negative (-2.41 percent). Production of egg by traditional management was found profitable enterprise in small and large categories of farms. As a result, residual share was positive (1.54 percent and 12.01 percent) for small and large farms. In case of commercial poultry production on an average the largest share current inputs (74.29 percent), residual ((19.64 percent), followed by family labor (5.64 percent) and depreciation (0.44 percent) respectively. In addition, on an average broiler production the largest share current inputs (84.18 percent), residual (12.53 percent), followed by family labor (2.68 percent) and depreciation (0.62 percent) respectively.

      PubDate: Sun, 07 Aug 2016 02:52:38 GMT
       
  • Effect of heat stress on milk production and its composition of Holstein
           Friesian crossbred dairy cows

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Mahmud Al Reyad, Md. Abid Hasan Sarker, Md. Elias Uddin, Raihan Habib and Md. Harun-ur-Rashid.
      The aim of this research was to observe the effect of heat stress on milk yield and milk compositions of Holstein Friesian crossbred (HF) dairy cows. To fulfill the objectives, a total of 9 Holstein Friesian crossbred cows were selected for this study. Green grasses (German, Para) were supplied adlibitum and concentrate feeds (mixture of wheat bran, rice polish, mustard oil cake, di-calcium phosphate and salt) were supplied at the rate of 2.0 kg/day/cow. Management practices for all the cows were similar following the BAU Dairy farm practices. Data were collected on milk yield (l/h/d), relative humidity (%) and barn temperature (0C). The obtained temperature humidity index (THI) of July, August, September and October were 84.95, 81.99, 81.40 and 79.57, respectively. The highest THI was found in July which indicated higher heat stress during this month. A significant difference (p

      PubDate: Sun, 07 Aug 2016 02:46:55 GMT
       
  • Effect of lead acetate alone and in combination with whole milk (Star
           ship®) on body growth and liver functions in an experimentally induced
           lead toxicity in rat

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Mohammad Usman Gani, Md. Saiful Islam Siddiqui, Md. Harun-or-Rashid, Kamrul Islam, Sharifunnessa Moonmoon, Shaifuddin Ahmed and Mahbub Mostofa.
      The Effect of lead acetate alone and in combination with whole milk on body weight gain and some biochemical parameters were carried out on a total of 15 (15 days old) male weaning Long- Evans strain rats. The rats were randomly divided into three equal groups, each consisting of five rats. Rats of group A were kept as control (without giving any treatment), group B received lead acetate alone @ 6mg/ml drinking water and group C received lead acetate @ 6mg/ml plus whole milk (Star ship®) 150 mg/ml drinking water. The result showed that body weight gain of control group per week per rat was found to increase but in treated group B, the body weight gain was found to decrease most significantly (P< 0.01) on day 56 while in group C, body weight was reduced significantly (P< 0.05) on day 56. The reducing body weight gain was less in group C than group B. A most significantly (P< 0.01) increased SGOT and SGPT values were observed in Group B but in group C, those count increased significantly (P< 0.05) on day 56 of experiment. From the study it was concluded that treatment with lead acetate at low doses has adverse effects on body growth and liver functions in experimental animals.

      PubDate: Sun, 07 Aug 2016 02:41:49 GMT
       
  • Molecular detection of cattle and buffalo species meat origin using
           mitochondrial cytochrome b (Cyt b) gene

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Sirazum Munira, Fatema Tuz Jahura, Md. Munir Hossain and Mohammad Shamsul Alam Bhuiyan.
      The study was conducted to adopt PCR based technique for identification of species origin from meat samples of cattle and buffalo using mitochondrial cytochrome b (Cyt b) gene fragment. A total of 42 ear tissue and meat samples were collected from different slaughterhouses and farms of Mymensingh, Bogra and Rangpur districts and stored in 96% ethanol at room temperature. Genomic DNA was extracted from all samples using GeNet Bio genomic DNA isolation kit. The average DNA yield of considered samples was found 204.57 ng/µl where the purity ranged from 1.82–1.99. Two (2) pair species-specific primers were used to amplify Cyt b gene fragments of 472 bp and 124 bp for cattle and buffalo, respectively. The PCR results revealed different species specific amplified fragments which could discriminate between cattle (472 bp) and buffalo (124 bp) species precisely from pure and mixed samples of those species. This study suggests an accurate molecular technique for identification of cattle and buffalo species meat origin and differentiates species present in adulterated meat samples. In conclusion, this DNA based technique could be utilized for prevention of malpractice in slaughterhouse and chain shops and thereby to protect consumer’s right.

      PubDate: Sun, 07 Aug 2016 02:38:47 GMT
       
  • Prevalence of Salmonella and Escherichia coli contamination in shrimp
           (Penaeus monodon) farms, depots and processing plants in different areas
           of Bangladesh

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Faridullah, Vikash Chandra Roy and Ulfat Jahan Lithi.
      A study was conducted to determine the level of contamination by indicator organisms (Salmonella and Escherichia coli) in shrimp (Penaeus monodon) farms, depots and processing plants of Cox’s Bazar, Khulna, Bagerhat and Satkhira districts of Bangladesh using conventional technique of bacterial isolation and identification. The results of this study revealed that prevalence of Salmonella positive samples was 43.7%, 62.5%, 20% and 0.0% for water, pond scum, shrimp and basket samples, respectively at farm level. Meanwhile, the prevalence of E. coli positive samples was 62.5%, 43.7%, 60% and 60% for water, pond scum, shrimp and basket samples, respectively at farm level. In case of depots, shrimp, basket and mat samples were analyzed and found 20%, 56.3% and 23% positive for Salmonella and 53.3%, 37.5% and 92.3% positive for E. coli. Bacteriological assessment of the shrimp samples obtained from four seafood processing plants revealed that all the samples were found contaminated with Salmonella and E. coli except the samples of one industry. Investigation finally showed that with few exceptions all the samples from farms, depots and processing plants have different levels of contamination by Salmonella and E. coli, which is very much alarming for shrimp industry of Bangladesh.

      PubDate: Sun, 07 Aug 2016 02:36:20 GMT
       
  • Changes in hepatosomatic index and histoarchitecture of liver in common
           carp exposed to organophosphate insecticide sumithion

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Sabrina Hossain, Md. Idris Miah, Md. Sadiqul Islam and Md. Shahjahan.
      Sumithion is widely used in larval rearing aquaculture ponds to control aquatic insects. This experiment was carried out to evaluate the effect of sumithion on hepatosomatic index and liver morphology in common carp, Cyprinus carpio. Acute toxicity of sumithion (96 h LC50 value) was first determined and it was 8.05 ppm for common carp. The fish were exposed to two sub-lethal concentrations (0.85 and 1.7ppm) of sumithion for a period of 21 days. A control set was also run for the same time with the same number of fish without sumithion (0 ppm). There was a marked increase in the hepatosomatic index in both concentrations (0.85 and 1.70 ppm) compared to control (0 ppm). Several histological changes of liver, such as necrosis, patchy degeneration, degenerated hepatocytes, vaculation and blood spilling were observed after exposure to sumithion with dose and exposure time dependent manner. The present investigation revealed the toxic potentiality of sumithion on common carp which alters liver morphology that may lead metabolic changes in fish.

      PubDate: Sun, 07 Aug 2016 02:26:34 GMT
       
  • Therapeutic use of Adhatoda vasica

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Tofazzal Hossain and Md. Obydul Hoq.
      Adhatoda vasica Nees belonging to family Acanthaceae, commonly known as Adosa, is found many regions of India and throughout the world, with a multitude of uses in traditional Unani and Ayurvedic systems of medicine. It is also called “Vasaka”. It is a well-known herb in indigenous systems of medicine for its beneficial effects, particularly in bronchitis. Vasaka leaves, bark, the root bark, the fruit and flowers are useful in the removal of intestinal parasites. Vasaka herb is used for treating cold, cough, chronic bronchitis and asthma. In acute stages of bronchitis, vasaka gives unfailing relief, especially where the sputum is thick and sticky. It liquefies the sputum so that it is brought up more easily. For relief in asthma, the dried leaves should be smoked. The juice from its leaves should be given in doses of 2 to 4 grams in treating diarrhea and dysentery. A poultice of its leaves can be applied with beneficial results over fresh wounds, rheumatic joints and inflammatory swellings. A warm decoction of its leaves is useful in treating scabies and other skin diseases. In olden times its leaves were made into a decoction with pepper and dried ginger. But the modern medicine searched its active ingredients and found out that vasicine, oxyvascicine and vasicinone are the alkaloids present in vasaka and in which vasicine is the active ingradient for expelling sputum from the body.

      PubDate: Sun, 07 Aug 2016 02:23:03 GMT
       
  • Therapeutic use of Withania somnifera

    • Abstract: 2016-08-10T04-18-05Z
      Source: Asian Journal of Medical and Biological Research
      Md. Khorshed Alam, Md. Obydul Hoq and Md. Shahab Uddin.
      Withania somnifera (L) Dunal is commonly called as Ashwagandha/Asgand and it belongs to the family Solanaceae. It is a well known medicinal plant widely used in the treatment of many clinical conditions in Indian subcontinent. It is an important medicinal plant commonly known as Asgand which has been used either single or in combination with other drugs in Unani as well as Ayurvedic system of medicine for centuries. It has been described by Dioscorides (78 AD) in his book “Kitab-ul-Hashaish”. Asgand consists of the roots of Withania somnifera which has various therapeutic actions such as anti – inflammatory (Muhallil-e-Warm), sedative (Musakkin), aphrodisiac (Muqawwi-e-Bah), hepatoprotective, immune-modulator activity, antioxidant, anti-ageing, anti-tumour activity, adaptogenic activity and has anti-anticonvulsant activity. Keeping in view the medicinal properties of Withania somnifera Dunal (Asgand), an attempt has been made in this paper to explore various dimensions of the drug including phytochemical and pharmacological studies carried out on this herb.

      PubDate: Sun, 07 Aug 2016 02:20:41 GMT
       
  • Prevalence of canine parvovirus and canine influenza virus infection in
           dogs in Dhaka, Mymensingh, Feni and Chittagong districts of Bangladesh

    • Abstract: 2016-07-25T23-15-29Z
      Source: Asian Journal of Medical and Biological Research
      Soumitra Sen, Md. Siddiqur Rahman, Minakshi Nag, Mohummad Muklesur Rahman, Roma Rani Sarker and S. M. Lutful Kabir.
      Canine parvovirus (CPV) and canine influenza virus (CIV) are highly contagious virus infecting members of the canine family including dogs, coyotes, foxes and wolves. The research work was carried out to determine the prevalence of CPV and CIV in dogs (stray and pet dogs) in Dhaka, Mymensingh, Feni and Chittagong districts by using RapiGen CPV and CIV Ag test kit. Fecal samples were collected from 50 dogs of different age and sex from different spots of Dhaka and Chittagong districts. Nasal fluid samples were collected from 50 dogs of different age and sex from different spots. The samples were tested with RapiGen canine parvovirus Ag test kit and RapiGen canine influenza Ag test kit. Overall prevalence of CPV in pet dogs was 22% and in stray dogs was 30%. The prevalence of canine parvovirus in relation to age was gradually decreasing with higher age group and the prevalence was 28% in 1-6 months age, 16.66% in 7-12 months age group 11.11% over 18 months age group. The prevalence of canine parvovirus was significantly higher in male (24.13%) than that in female (19.04%). All samples showed negative test result for canine influenza virus. Prevalence of canine influenza virus infection were 00.00% in all age groups and sex. Overall prevalence of CIV in all pet and stray dogs were 00.00%.

      PubDate: Wed, 20 Jul 2016 04:59:32 GMT
       
  • Management of seed borne fungal pathogens of okra collected from seed
           companies

    • Abstract: 2016-07-25T23-15-29Z
      Source: Asian Journal of Medical and Biological Research
      G. M. Kibria Hossain, S.M. Ahsan and Tanjila Ahmed.
      Effect of mehogoni, mehedi and allamanda extracts were tested to control seed borne fungi of okra seeds collected from 6 companies of notunbazar in Mymensingh district. Prevalence of seed borne fungi was studied by blotter method in the Seed Pathology Center (SPC) and MS Laboratory, Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh. The highest germination percentage was recorded from ACI seeds (88%), while lowest (70%) in BADC seeds. Six predominant fungal genera were identified. These species were Fusarium oxysporum (5.08%), Aspergillus flavus (4.50%), Aspergillus niger (6.50%), Colletotrichum dematium (4.67%), Rhizopus stolonifer (3.33%) and Penicillium spp. (3.00%). Germination percentage and fungal association varied from company to company. The germination was ranged from 70-95% and infections were recorded 0.80-6.1% in all the treated seeds. Mehogoni extracts at the rate of 1:1 showed best performance in increasing seed germination (96.00%) next to allamanda (70%). Vigour index of okra seeds were increased 19.14% over untreated seeds by the treatment of mehogoni seed extracts at the rate of 1:1. Mehogoniseed extract at the rate of 1:1 seemed to be adoptable at the farmer‟s level as an organic management practice.

      PubDate: Wed, 20 Jul 2016 04:55:50 GMT
       
  • Assessment of nutritional composition and heavy metal content in some
           edible mushroom varieties collected from different areas of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      K.M. Mohiuddin, Md. Mehediul Alam, Md. Taufique Arefin and Istiaq Ahmed.
      Four edible mushroom species (Pleurotus ostreatus, Agaricus bisporus, Volvariella volvacea, Ganoderma lucidum) from different locations of Bangladesh, were analysed for their protein and metal content profile (K, Na, Fe, Cu, Zn, Mn, Cr, Pb, As and Cd). Trace metals were determined by atomic absorption spectrophotometer, Na and K by flame emission spectrophotometer and protein by micro Kjeldhal method. All element concentrations were determined on a dry weight basis. The protein content of mushrooms varied from 13.8%–34.3% and the metal content of samples ranged from 0.54–2.25% for K and 12.6–81.6, 69.5–626.2, 39.2–163.4, 30.1–75.5, 52.9–104.5, 0.20–0.30, 0.13–0.59 μg g-1 for Na, Fe, Cu, Zn, Mn, Cd, Pb, respectively. Arsenic and cadmium concentrations were below the detection limit of the method used. The detection limits of the method for As and Cd are 0.01 μg g-1 for each element. In general, K and Fe content were higher than other metals in all mushroom species. The levels of Cu and Zn in some mushroom samples were found to be higher than legal limits.

      PubDate: Sun, 17 Jul 2016 07:17:45 GMT
       
  • An efficient protocol for in vitro regeneration of Stevia rebaudiana

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sheikh Rashel Ahmed, Md. Moniruzzaman Shohag Howlader, Pijush Sutradhar and Sabina Yasmin.
      An efficient high frequency plant regeneration protocol through direct organogenesis was developed for Sevia rebaudiana. Shoot tips containing axillary buds were used as an explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.6% (w/v) agar supplemented with various concentrations of benzy-ladenine (BA), kinetin (Kn) and thidiazuron (TDZ).. BAP proved to be a better choice than Kn and the maximum number of shoots (3.75) was obtained on 2.0 mgL-1 BAP concentration. Considering all parameters, combination of BAP and Kn gave comparatively better performance than single BAP or Kn. TDZ was effective for multiple shooting. Though, 1.5 mgL-1 TDZ gave the best number of shoots (14.5), but 1.0 mgL-1 TDZ gave best performance in response to all parameters under study. The highest number of shoots was obtained in 60 mlL-1 coconut water, but 40 mlL-1 coconut water gave the best result to all parameters. Root induction was tested by using two auxins namely NAA and IBA at different concentrations (1.0, 1.5, 2.0 mgL-1) on the MS medium. IBA at 1.0 mgL-1 increased the rooting response (66.67%), number of roots (7.0) and root length (2.9 cm). Higher concentration of IBA and NAA (2.0 mgL-1) showed poor results of rooting response (33.33%).

      PubDate: Sun, 17 Jul 2016 07:02:54 GMT
       
  • Yield attributes and oil content of different mustard (Brassica campestris
           l.) varieties effected by different levels of fertilizers

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Kamol Kumar Barman, Sakil Mahmud, Muhammad Salim and Bishan Lal Das Chowdhury.
      An experiment was conducted at the Agronomy Field Laboratory and Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh during the period from November 2013 to February 2014 to find out the appropriate fertilizer dose and best variety on the yield and oil content of mustard (Brassica spp). The experiment consisted of four fertilizer treatments viz., 0 fertilizer dose (control), 50% of recommended fertilizer dose, 100% recommended fertilizer dose and 150% of recommended fertilizer dose and three varieties viz. BINA Sarisha-5, BINA Sarisha-8 and Tori-7. The experiment was laid out in a two factor randomized complete block design with three replications. Both fertilizer dose and variety had significant effect on plant height (cm), number of branches plant-1, number of siliqua plant-1, siliqua length (cm), number of seeds siliqua-1, 1000 seed weight (g) and oil content (%). The interaction of fertilizer dose and variety had significant effect on all the plant characters except oil content of mustard (Brassica spp). The best result of the above characters was recorded in 100% recommended fertilizer dose in combination with BINA Sarisha-8. The next best result was obtained from 100% recommended fertilizer dose in combination with BINA Sarisha-5.

      PubDate: Sun, 17 Jul 2016 03:57:55 GMT
       
  • Impact of wastewater irrigation on major nutrient status in soil near
           Bhaluka industrial area of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Abdullah-Al-Zabir, M.W. Zaman, Md. Zakir Hossen, Md. Nizam Uddin, Md. Jamil Hossain Biswas and Abdulla-Al-Asif.
      The population increase has not only increased the fresh water demand but also increased the volume of wastewater generated. Treated or recycled wastewater (RWW) appears to be the only water resource that is increasing as other sources are dwindling. Increasing need for water has resulted in the emergence of domestic wastewater application for agriculture and its relative use. The present study was conducted at the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh during 2013 to evaluate the contribution of wastewater to major soil nutrients (N, P, K, S, Ca, Mg, B and Na) and fluctuation in physicochemical properties of soil (soil pH and Ec) from waste carrying canal at 10 selected sites of Bhaluka Upazila. Three (3) soil samples were collected at 0, 30 and 60 m distances from the waste discharging canals. The pH, EC, N, P, K, S, Ca, Mg, B and Na in soil samples decreased gradually with the increase of distance from waste discharging canal. Maximum concentrations of N at 60 and 0m distance varied from 8400 to 9700, P from 1850 to 5000, K from 4600 to 6000, S from 2000 to 4000, Ca from 7500 to 28800, Mg from 7500 to 7800, B from 90 to 2800 and Na from 2300 to 3100 μg g-1 in test soil.The results showed better nutrient status of the soil along waste discharge canals. The findings give applicable advice to commercial farmers and agricultural researchers for proper management and use of treated industrial wastewater for agricultural purpose.

      PubDate: Sun, 17 Jul 2016 03:47:17 GMT
       
  • Biochemical and phytochemical evaluation of Stevia rebaudiana

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Moniruzzaman Sohag Howlader, Sheikh Rashel Ahmed, Khadizatul Kubra and Md. Khairul Hassan Bhuiyan.
      The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.

      PubDate: Sun, 17 Jul 2016 03:44:54 GMT
       
  • Morphometric study of banded gourami (Colisa fasciata) in Jessore,
           Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sharmin Akter, Md. Farid Uz Zaman, Md. Hasan-Uj-Jaman, Iren Nahar Sithi, Dilruba Yesmin and Abdulla-Al-Asif.
      Population morphometry including population demography, size group distribution, length weight relationship and sex ratio of an important freshwater fish, Colisafasciata was studied which collected fromJessore and Jhenaidah districts in Bangladesh during May, 2014 to April, 2015. Random samples of 201 individuals (108males and 93 females) were collected from different fish markets in Jessore (Monirampur, Borobazar, Chowgasa) and Jhenaidah (Mohespur, Barobazar, Kotchandpur) districst. The mean size of the specimen was recorded 65.08 ±14.32 mm in length and 7.43 ±3.09 g in body weight. Females were found little smaller than males. The specimens were larger in winter than summer and monsoon season. But in monsoon season the females were larger than males. The maximum length of the specimen collected from Jhenaidah district was smaller compare to the specimen of Jessore district. Most of the population (29%) was observed under 55-64 mm size class. Linear relationship was obtained between the log length (mm) and log weight (g) of the species in respects of sexes, seasons, and districts. The allometric coefficient ‘b’ of the relationship indicated negative allometric growth of the species as ‘b’ value was less than 3. The overall sex ratio (male: female=1:0.86)showed significant difference from the expected value 1:1. Overall males showed non-significant over females (χ2 =1.119) in all season of both districts but in November males showed the significant (P

      PubDate: Sun, 17 Jul 2016 03:42:30 GMT
       
  • Prevalence of poultry diseases in Gazipur district of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Kamrul Hassan, Md. Humayun Kabir, Md. Abdullah-Al-Hasan, Shobnom Sultana, Md. Shohidul Islam Khokon and S. M. Lutful Kabir.
      This study was designed with a view to investigate the prevalence of poultry diseases in Gazipur district of Bangladesh. A total of 679 poultry birds (313 layers, 338 broilers and 28 cockrels) either dead or live were brought for diagnosis of diseases at Gazipur Sadar Upazilla Veterinary Hospital. The diseases were diagnosed on the basis of history, clinical signs and post-mortem findings. The diseases encountered in layers were bacterial diseases 52.29% (salmonellosis 38.56%, colibacillosis 6.7%, fowl cholera 4.79% and necrotic enteritis 1.60%), viral diseases 23.95% (avian influenza 2.56%, Newcastle disease 16.61%, infectious bronchitis 3.19% and avian leucosis 0.64%), mycoplasmal disease (mycoplasmosis 14.70%) and protozoal disease (coccidiosis 5.75%). Salmonellosis was most prevalent disease in age group of>20 weeks, while Newcastle disease most common in 8 to 20 weeks of age group. In case of broiler, bacterial diseases 28.99% (salmonellosis 21.30% and colibacillosis 7.69%), viral diseases 53.24% (infectious bursal disease 28.99%, Newcastle disease 8.87% and infectious bronchitis 15.38%), mycoplasmal disease (mycoplasmosis 7.1%) and protozoal disease (coccidiosis 6.5%). In cockrels, the most prevalent disease was colibacillosis 35.71% followed by salmonellosis 28.57%, Newcastle disease 14.28% and mycoplasmosis14.28%. So among the diseases, salmonellosis is most prevalent disease followed by infectious bursal disease and mycoplasmosis in different kinds of poultry of Gazipur district of Bangladesh.

      PubDate: Sun, 17 Jul 2016 03:39:47 GMT
       
  • Epidemic behavior of the etiological agent of infectious coryza in layer
           chicken of Bangladesh with isolation, identification and pathogenicity
           study

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mir Rowshan Akter, Md. Shahidur Rahman Khan, Md. Mostafizer Rahman, S. M. Lutful Kabir and Md. Abu Sayed Khan.
      The present study was selected as infectious coryza is one of the major problems affecting poultry industry in the developing country like Bangladesh and the reports regarding infectious coryza are yet not be documented considering epidemiological investigation, proper isolation, identification and pathogenicity study. The epidemic behavior of the etiological agent of this disease were studied based on age, sex, breed, spatial and temporal differences after collection of samples suspected to be infected with infectious coryza in layer chicken of Bangladesh. The incidence rate of infectious coryza from field cases were recorded as per information received from farmers by using a structured questionnaire and also clinical signs and symptoms. The disease was very high in laying hen (18.38%) in Sylhet and growing birds (7.25%) in Khulna in comparing with prelaying stage (2.07%) also in Sylhet region of Bangladesh. In this study no significant differences was observed as their location variation except Sylhet (9.2%) in comparison with other areas (Rangpur – 8.76%, Rajshahi -8.82%, Khulna – 8.83%, Dhaka – 8.72 and Chittagong – 8.65% respectively) of Bangladesh but significant differences was observed as their age group. However, the incidence rate of this disease was found to be very high during winter (8.77%) in compare with summer (0.42%) season. Moreover, during investigation a total of 122 samples were collected from different areas of Bangladesh for the period of March 2011 to February 2014. The higher rate of incidence of A. paragallinarum was found in Sylhet (66.66%) and lowest in Dhaka (43.75%). The association of A. paragallinarum with different seasons revealed that higher incidence rate was found in winter season (52.26%) in comparison with summer season (1.85%). The suspected positive isolates were subjected to experimental pathogenicity study in natural host for there – isolation of A. paragallinarum was done as per Kotch postulates.

      PubDate: Sun, 17 Jul 2016 03:24:07 GMT
       
  • Mitigation of salt stress in rice plant at germination stage by using
           methyl jasmonate

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sakil Mahmud, Shayla Sharmin, Bishan Lal Das Chowdhury, Mohammad Anowar Hossain and Muhammad Javidul Haque Bhuiyan.
      To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 μM MeJA and saline (12 dSm-1) + 20 μM MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 μM had a better effect over 20 μM MeJA.

      PubDate: Sun, 17 Jul 2016 03:21:34 GMT
       
  • Socio-economic condition of fishermen and intermediaries involved in
           marine fish marketing chain in Cox’s Bazar area, Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Khabirul Ahsan, Nusrat Sultana Runa, Subrata Kumar Ghosh, Muhammad Mehedi Hasan and Md. Kamal.
      Study was conducted to know the socio-economic condition of the fishermen and intermediaries of fish marketing chain in Cox’s Bazar area, Bangladesh. Socio-economic condition of the fishermen and intermediaries were not so good in the study area. It was found that about 35% fishermen and intermediaries were illiterate, 16% fishermen and intermediaries were semiliterate who only could write their names. 55% household of the fishermen and intermediaries had tin shed house with tin wall. Average income for most of fishermen and intermediaries was about Tk. 400-500 and 66% were found to borrow money from different source for continuing their business. Poor road and transport facilities, lack of credit facilities, insufficient supply of ice, inadequate training facilities and large number of intermediaries in the chain were the main problems of the marine fish marketing chain. It is therefore necessary to provide institutional and organizational support, government support, extension service and training facilities to the market operators for sustainable fish marketing systems.

      PubDate: Sun, 17 Jul 2016 03:19:41 GMT
       
  • A surveillance study on dairy farmers selected in Baghabari milk pocket
           area of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Nuruzzaman Munsi, Md. Azharul Islam Talukder, Md. Zillur Rahman and Md. Abu Hemayet.
      This study was carried out to reveal the animal agriculture status of the dairy farmers. The survey was conducted by random sampling on 100 dairy farmers (50 from Shahzadpur upazila and 50 from Bera upazila). A pretested questionnaire was developed and multiplied for data collection. The data collected were brought to the headquarters of Bangladesh Livestock Research Institute, Savar, Dhaka for analysis. After analysis it was found that the education level of the farmers of Shahzadpur upazila was better than that of Bera upazila, where 74% farmers of Shahzadpur and 66% farmers of Bera were engaged in agriculture. The average annual income and expense of the farmers of Shahzadpur were greater than that of Bera. The average total land, cultivable land and total livestock population per farmer were also higher in Shahzadpur upazila than in Bera upazila. In case of diversity of different genotypes of dairy cattle, except Jersey cross the number of Pabna, Sindhi cross, Shahiwal cross and H-F cross was much greater in Shahzadpur than in Bera. Most of the farmers purchase concentrate feeds and produce roughages in both the locations, where feed scarcity was seen mostly during rainy season. About 56% farmers treat their cattle with the help of veterinary surgeon and 44% farmers provide treatment with the help of veterinary field assistant in Shahzadpur, while the scenery was almost opposite in Bera. About 90% farmers of Bera upazila and 95% of Shahzadpur upazila used anthelminitics to deworm their animals. Most of the farmers in both sites dewormed their cattle every three months. About 78% farmers of Shahzadpur and 76% of Bera vaccinated their animals. About 85% farmers of Shahzadpur had been found to properly dispose the carcasses, where as only 48% farmers had been found to do it in Bera. Almost all the farmers washed their hands, equipment and udders of the cows with clean water before milking in both Shahzadpur and Bera. Approximately, all the farmers of both the locations milked their cows twice a day, did not perform milk testing and sold the raw fresh milk to the cooperative societies. All farmers used cow dung as fuel. Overall, it was revealed that Shahzadpur upazila was more advanced than Bera upazila in almost all aspects of animal agriculture.

      PubDate: Sun, 17 Jul 2016 03:16:52 GMT
       
  • Effects of saline water on health status of pregnant women in coastal
           regions of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Zohirul Islam Rony, Md. Shafiqul Islam Khan, Md. Ali Asgar, Musammet Rasheda Begum and ASM Golam Kibria.
      Drinking water from natural sources in coastal region of Bangladesh has been contaminated by varying degrees of salinity which have detrimental effects on the health. Pregnancy itself is a crucial time for women and the salinity increases its vulnerability regarding the raising of blood pressure. The study was conducted to evaluate the effect of saline water intake on the health status of pregnant women at Barguna and Patuakhali districts, Bangladesh. Sodium (Na) concentration in urine samples was analyzed and corresponding blood pressure was taken in the diagnostic laboratory. Descriptive statistical analysis showed that Na concentration, systolic and diastolic pressure (BP) was the highest in 2nd trimester followed by 3rd trimester and 1st trimester. Water intake and urine production were the highest in 3rd trimester and increased with age. Na concentration, systolic and diastolic pressure (bp) was the highest in 3rd parity followed by 2nd parity. Water intake and urine production did not very among parities. Correlation matrix express that sodium concentration has the significant association with the age and the highly significant association with systolic and diastolic blood pressure. In Logistic Regression Model, odds ratio (OR) estimate showed that women with systolic bp≥120 had 1.109 times greater sodium concentration than with systolic blood pressure of

      PubDate: Sun, 17 Jul 2016 03:13:45 GMT
       
  • Poverty alleviation of destitute women and poor farmers in Barisal
           district of Bangladesh through beef fattening

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mohammad Rohul Amin, Mohammad Hafizur Rahman, Milton Talukder, Md. Ali Asgar, Ashit Kumar Paul and Mohammad Lalmoddin Mollah.
      The cattle fattening programme was represents an important component of the agribusiness sector with great economic, income, poverty alleviation and social implications. The present study was performed at Barisal district in Bangladesh. Data were collected from 220 women and farmers on their household during the period June, 2013. Among them, 54 destitute women and poor farmers were identified and making groups of them for beef fattening. 54 cattle of each destitute women and poor farmers were randomly divided into nine groups (A, B, C, D, E, F, G, H and I), each consisting of six (6) cattle. Endex®, Aldazole® and Tetranid® were administered orally to the cattle of group A, B and C, respectively. Cattle of group D received subcutaneously Oxynil®. Cattle of group E received orally Peraclear® and Urea Molasses Straw (UMS). Cattle of group F received orally Endex® and lugols iodine intravenously. Cattle of group G were treated orally with Endex® and Metaphos® intramuscularly. Cattle of group H were treated orally with Endex® and A-Sol® intramuscularly. Cattle of group I was kept as control without giving any treatment. Body weight was examined before trials (day 0) and on 10th day, 20th day, 30th day, 40th day, 50th day, 60th day and 70th day. Out of the 220 women and farmers 56.36% were involved in agriculture, 10.45% in own business, 3.64% in shared business, 6.36% in govt. service and 23.18% in non-govt. service. 18.18% women and farmers had the knowledge of animals characteristics used for beef fattening. The knowledge of feed, animal diseases, veterinary drugs, animal vaccine, and drugs used for beef fattening was 18.64%, 47.73%, 25.45%, 25.45%, and 19.55%, respectively. About 7.73% respondents have training on cattle fattening. The body weight was increased significantly (p

      PubDate: Sun, 17 Jul 2016 03:11:22 GMT
       
  • Effect of arsenic trioxide along with tannic acid, di-sodium hydrogen
           phosphate, alum and effects of sand-charcoal-iron-filter bed filtrated
           water along with alum on body weight and some hematological parameters in
           rabbit

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Shaifuddin Ahmed, Md. Saiful Islam Siddiqui, Kamrul Islam, Mohammad Usman Gani, Sharifunnessa Moonmoon, Md. Harun-or-Rashid and Md. Abdul Awal.
      The effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and effects of sand-charcoal-iron-filter (SCIF)-bed filtrated water along with alum on body weight and some hematological parameters were carried out on a total of 30 (01 month old) adult Newzealand white rabbits. The Rabbits were randomly divided in to 6 equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100 ppm, group C received arsenic trioxide @ 100 ppm plus tannic acid @100 ppm, group D received arsenic trioxide @ 100 ppm plus di-sodium hydrogen phosphate @100 ppm, group E received arsenic trioxide @100 ppm plus alum @100 ppm orally daily for 60 days in all cases and group F received alum @100 ppm in SCIF-bed filtrated water orally daily for 60 days. The result showed that body weight gain of control group (A) per week per rabbit was found to increase but in treated group B (arsenic trioxide@100ppm orally daily) the mean body weight of group B were decreased gradually and significant (P

      PubDate: Sun, 17 Jul 2016 03:07:26 GMT
       
  • Sero-surveillance and sero-monitoring of locally produced PPR vaccine in
           the field and experimental level

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Ehsanul Kabir, Md. Mokbul Hossain, Md. Ershaduzzaman, Md. Abu Yousuf and Md. Rafiqul Islam.
      Peste des Petits Ruminants (PPR) is a highly contagious, economically important viral disease of goats with high morbidity and mortality. To control the disease effectively a live attenuated vaccine is available in Bangladesh which is produced by Livestock Research Institute (LRI), Mohakhali, Dhaka. The study was carried out to determine the immune status and immune response against PPR in field and experimental Black Bengal goats. Sero-surveillance of PPR was conducted by using c-ELISA in non-vaccinated 240 goats in Gazipur, Sirajgonj and Barisal. Out of the 240 goats tested, of which only 39 (20.31%) goats had positive level of PPR antibodies while 16.25% (13 out of 80 goats) in Gazipur, 28.75% (23 out of 80 goats) in Barisal and 3.75% ((3 out of 80 goats)) in Sirajgonj. In case of sero-monitoring of PPR, the result revealed that vaccinated goats from Rajshahi showed high positive result and have higher seroprevalence where 75% (60 out of 80 goats) were seropositive and only 25% (20 out of 80 goats) are seronegative. These result indicated that vaccinated Rajshahi goats is more resistant for PPR virus than non vaccinated goats. In experimentally to perform sero-monitoring, 10 seronegative goats were selected and divided into two equal groups (A and B).The immunization study against PPR with a commercial PPR vaccine was conducted on 5 goats of group A by inoculating @ 1.0 ml vaccine / animal subcutaneously and group B kept as non-vaccinated. The antibody titres against PPR in goats were determined at 0 day on vaccination and after 21DPV, 180DPV and 365DPV. The results found that 100% (5 out 5goats) seronegative in both vaccinated goats of group A and non-vaccinated goats of group B at 0 day on vaccination. The mean negative titres± SD were 79.285±13.921 and 76.707±9.265 in vaccinated group A and group B, respectively. The mean positive titers ±SD were 20.201±2.480, 8.630±4.970 and 11.382±1.419 at 21DPV, 180DPV an 365DPV, respectively in group A (100% seropositive). In case of non-vaccinated group B, the mean negative titres±SD were 74.258±7.793, 77.726±9.142 and 82.965±7.492 at 21DPV, 180DPV and 365DPV, respectively (100% seronegative). As it is observed, the antibody titres remain at the level over the period of time that indicates the immune response against PPR. From this finding, it is said that PPR vaccine could produce immune response in goats for about one year or 365 days.

      PubDate: Sun, 17 Jul 2016 03:04:22 GMT
       
  • Identification of genera of tubificid worms in Bangladesh through
           morphological study

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mariom, Sharmin Nahar Liza and Md. Fazlul Awal Mollah.
      Tubificids are aquatic oligochaete worms (F- Naididae, O- Haplotaxida, P- Annelida) distributed all over the world. The worms are very important as they are used as live food for fish and other aquatic invertebrates. A step was taken to identify the genera of tubicifid worms that exist in Mymensingh district, Bangladesh on the basis of some external features including the shape of their anterior (prostomium) and posterior end, number of body segment and arrangement of setae. The study result indicated the existence of three genera among the tubificid worms. These were Tubifex, Limnodrilus and Aulodrilus. All these three genera possessed a cylindrical body with a bilateral symmetry formed by a series of metameres. The number of body segments ranged from 34 to 120 in Tubifex, 50 to 87 in Limnodrilus, and 35 to 100 in Aulodrilus. In Tubifex, the first segment, with the prostomium, was round or triangular bearing appendages, whereas, in Limnodrilus and Aulodrilus, the prostomium without appendages was triangular and conical, respectively. Three types of setae i.e., hair setae, pectinate setae and bifid setae of various sizes were found in Tubifex, whereas, Limnodrilus possessed pectinate setae and bifid setae of more or less similar size and shape; and Aulodrilus possessed only hair setae and bifid setae of different size.

      PubDate: Sun, 17 Jul 2016 03:01:34 GMT
       
  • Evaluation of nutritive value and shelf life of strawberry genotypes

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sushan Chowhan, Md. Shyduzzaman Roni, Shaikh Shakib Ahmed, Hanif Uddin and Md. Mahmudul Hasan.
      An investigation was carried out to evaluate the nutritive value and shelf life of five strawberry genotypes. It was found that under fresh condition the highest ascorbic acid (45.76 mg/100g) was obtained by Rabi-3 and the highest -carotene (15.09 IU/100g) by Festival. The maximum amount of reducing sugar (6.373 g/100g) and non-reducing sugar (2.947 g/100g) was recorded in Camarosa and Festival respectively. The maximum amount of calcium (0.480%) in BADC Strawberry, iron (93.50 ppm) in Rabi-3, phosphorus (0.081%) and potassium (0.854%) in BARI Strawberry-1 was recorded. Under different temperature and packaging conditions, polyethylene package at 40C proved to be superior to others in all respects. The highest shelf life (47.00 days) and ascorbic acid (42.76 mg/100g) after four days of storage was obtained by Camarosa in this condition; whereas the lowest shelf life (2.33 days) and ascorbic acid (10.12 mg/100g) was recorded in BADC Strawberry in plastic punnet package at 250C.

      PubDate: Sun, 17 Jul 2016 02:58:58 GMT
       
  • Seroprevalence of ovine brucellosis in Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Osman Gani, Md. Nuruzzaman Munsi, Md. Ershaduzzaman, A.K.M. Anisur Rahman, Sajeda Sultana and Md. Shahin Alam.
      An investigation was carried out to study the seroprevalence and risk factors for Brucella seropositivity in sheep in Bangladesh. For this, highly sheep populated ten different districts including all seven divisions of Bangladesh were selected where sheep of Panchagarh (151), Naogaon (101), Tangail (83), Noakhali (56), Sunamganj (52), Patuakhali (119) and Dhaka (75) were tested but sheep of Chapai Nawabganj, Khulna, Noakhali and Feni has under tested for Brucellosis. In the present study, seroprevalence of ovine brucellosis was tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme-Linked Immunosorbent Assay (ELISA). About 5% sheep might have got Brucella infection. The highest Brucella antibody was observed in sheep of Tangail district (8.4%) followed by the sheep of Savar (8.0%), where as sheep of Subarna Char and Kala Para were free from Brucella antibody. In relation to age and sex, adults (6.5%) were more positive than young (3.6%) and female (5.3%) were more susceptible than male (3.8%).

      PubDate: Sun, 17 Jul 2016 02:56:59 GMT
       
  • Feeding and breeding management system of goat at central and southern
           regions of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Panir Choudhury, Anguara Khatun, Md. Shamsul Hossain and Farukul Islam.
      Data were collected to study the feeding and breeding management system of goat from the villages of Mymensingh, Barguna and Patuakhali districts in Bangladesh through direct interviewing method using a pre-structured questionnaire. The research work was conducted from June to July 2014. Most of the goat rearing farmers in central and southern region of Bangladesh reared goats in semi-intensive system and kept their goats at goat’s house. Farmers took winter care and took bath of their goats during summer. Maximum farmers (82%) of central region supplied concentrate feed but 61% farmers of southern region did not supply concentrate to their goat. Farmers (82%) of central region provided particular feeder but 67.7% farmers of southern region did not provide feeder during concentrate feeding. Tube well (84%) was the main source of water of goat at central part of Bangladesh. But main sources of water in southern region were (46.3%) tube well and (51.2%) pond. All farmers of central region did not keep buck but only 19.5% farmers of southern region kept buck for breeding their female goat. Ninety two percent famers of central part of Bangladesh and 70.7% farmers of southern region used village buck for breeding their does. All buck keeping farmers at central region received service charge during breeding and they also practiced close breeding method. Most of (73.2%) the buck keeping farmers at southern region of Bangladesh received service charge of buck and they did not practice close breeding system. So, the present study might be suggested that farmers at central region were more careful of their goats in terms of feeds, feeding, water sources selection and watering but farmers at southern region were more aware about buck keeping and inbreeding depressions in their goats.

      PubDate: Sun, 17 Jul 2016 02:54:20 GMT
       
 
 
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