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Journal Cover Anatomica Karnataka - an International Journal
  [1 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2229-7545 - ISSN (Online) 2249-5398
   Published by ScopeMed Homepage  [98 journals]
  • Prevalence of canine parvovirus and canine influenza virus infection in
           dogs in Dhaka, Mymensingh, Feni and Chittagong districts of Bangladesh

    • Abstract: 2016-07-25T23-15-29Z
      Source: Asian Journal of Medical and Biological Research
      Soumitra Sen, Md. Siddiqur Rahman, Minakshi Nag, Mohummad Muklesur Rahman, Roma Rani Sarker and S. M. Lutful Kabir.
      Canine parvovirus (CPV) and canine influenza virus (CIV) are highly contagious virus infecting members of the canine family including dogs, coyotes, foxes and wolves. The research work was carried out to determine the prevalence of CPV and CIV in dogs (stray and pet dogs) in Dhaka, Mymensingh, Feni and Chittagong districts by using RapiGen CPV and CIV Ag test kit. Fecal samples were collected from 50 dogs of different age and sex from different spots of Dhaka and Chittagong districts. Nasal fluid samples were collected from 50 dogs of different age and sex from different spots. The samples were tested with RapiGen canine parvovirus Ag test kit and RapiGen canine influenza Ag test kit. Overall prevalence of CPV in pet dogs was 22% and in stray dogs was 30%. The prevalence of canine parvovirus in relation to age was gradually decreasing with higher age group and the prevalence was 28% in 1-6 months age, 16.66% in 7-12 months age group 11.11% over 18 months age group. The prevalence of canine parvovirus was significantly higher in male (24.13%) than that in female (19.04%). All samples showed negative test result for canine influenza virus. Prevalence of canine influenza virus infection were 00.00% in all age groups and sex. Overall prevalence of CIV in all pet and stray dogs were 00.00%.

      PubDate: Wed, 20 Jul 2016 04:59:32 GMT
       
  • Management of seed borne fungal pathogens of okra collected from seed
           companies

    • Abstract: 2016-07-25T23-15-29Z
      Source: Asian Journal of Medical and Biological Research
      G. M. Kibria Hossain, S.M. Ahsan and Tanjila Ahmed.
      Effect of mehogoni, mehedi and allamanda extracts were tested to control seed borne fungi of okra seeds collected from 6 companies of notunbazar in Mymensingh district. Prevalence of seed borne fungi was studied by blotter method in the Seed Pathology Center (SPC) and MS Laboratory, Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh. The highest germination percentage was recorded from ACI seeds (88%), while lowest (70%) in BADC seeds. Six predominant fungal genera were identified. These species were Fusarium oxysporum (5.08%), Aspergillus flavus (4.50%), Aspergillus niger (6.50%), Colletotrichum dematium (4.67%), Rhizopus stolonifer (3.33%) and Penicillium spp. (3.00%). Germination percentage and fungal association varied from company to company. The germination was ranged from 70-95% and infections were recorded 0.80-6.1% in all the treated seeds. Mehogoni extracts at the rate of 1:1 showed best performance in increasing seed germination (96.00%) next to allamanda (70%). Vigour index of okra seeds were increased 19.14% over untreated seeds by the treatment of mehogoni seed extracts at the rate of 1:1. Mehogoniseed extract at the rate of 1:1 seemed to be adoptable at the farmer‟s level as an organic management practice.

      PubDate: Wed, 20 Jul 2016 04:55:50 GMT
       
  • Assessment of nutritional composition and heavy metal content in some
           edible mushroom varieties collected from different areas of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      K.M. Mohiuddin, Md. Mehediul Alam, Md. Taufique Arefin and Istiaq Ahmed.
      Four edible mushroom species (Pleurotus ostreatus, Agaricus bisporus, Volvariella volvacea, Ganoderma lucidum) from different locations of Bangladesh, were analysed for their protein and metal content profile (K, Na, Fe, Cu, Zn, Mn, Cr, Pb, As and Cd). Trace metals were determined by atomic absorption spectrophotometer, Na and K by flame emission spectrophotometer and protein by micro Kjeldhal method. All element concentrations were determined on a dry weight basis. The protein content of mushrooms varied from 13.8%–34.3% and the metal content of samples ranged from 0.54–2.25% for K and 12.6–81.6, 69.5–626.2, 39.2–163.4, 30.1–75.5, 52.9–104.5, 0.20–0.30, 0.13–0.59 μg g-1 for Na, Fe, Cu, Zn, Mn, Cd, Pb, respectively. Arsenic and cadmium concentrations were below the detection limit of the method used. The detection limits of the method for As and Cd are 0.01 μg g-1 for each element. In general, K and Fe content were higher than other metals in all mushroom species. The levels of Cu and Zn in some mushroom samples were found to be higher than legal limits.

      PubDate: Sun, 17 Jul 2016 07:17:45 GMT
       
  • An efficient protocol for in vitro regeneration of Stevia rebaudiana

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sheikh Rashel Ahmed, Md. Moniruzzaman Shohag Howlader, Pijush Sutradhar and Sabina Yasmin.
      An efficient high frequency plant regeneration protocol through direct organogenesis was developed for Sevia rebaudiana. Shoot tips containing axillary buds were used as an explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.6% (w/v) agar supplemented with various concentrations of benzy-ladenine (BA), kinetin (Kn) and thidiazuron (TDZ).. BAP proved to be a better choice than Kn and the maximum number of shoots (3.75) was obtained on 2.0 mgL-1 BAP concentration. Considering all parameters, combination of BAP and Kn gave comparatively better performance than single BAP or Kn. TDZ was effective for multiple shooting. Though, 1.5 mgL-1 TDZ gave the best number of shoots (14.5), but 1.0 mgL-1 TDZ gave best performance in response to all parameters under study. The highest number of shoots was obtained in 60 mlL-1 coconut water, but 40 mlL-1 coconut water gave the best result to all parameters. Root induction was tested by using two auxins namely NAA and IBA at different concentrations (1.0, 1.5, 2.0 mgL-1) on the MS medium. IBA at 1.0 mgL-1 increased the rooting response (66.67%), number of roots (7.0) and root length (2.9 cm). Higher concentration of IBA and NAA (2.0 mgL-1) showed poor results of rooting response (33.33%).

      PubDate: Sun, 17 Jul 2016 07:02:54 GMT
       
  • Yield attributes and oil content of different mustard (Brassica campestris
           l.) varieties effected by different levels of fertilizers

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Kamol Kumar Barman, Sakil Mahmud, Muhammad Salim and Bishan Lal Das Chowdhury.
      An experiment was conducted at the Agronomy Field Laboratory and Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh during the period from November 2013 to February 2014 to find out the appropriate fertilizer dose and best variety on the yield and oil content of mustard (Brassica spp). The experiment consisted of four fertilizer treatments viz., 0 fertilizer dose (control), 50% of recommended fertilizer dose, 100% recommended fertilizer dose and 150% of recommended fertilizer dose and three varieties viz. BINA Sarisha-5, BINA Sarisha-8 and Tori-7. The experiment was laid out in a two factor randomized complete block design with three replications. Both fertilizer dose and variety had significant effect on plant height (cm), number of branches plant-1, number of siliqua plant-1, siliqua length (cm), number of seeds siliqua-1, 1000 seed weight (g) and oil content (%). The interaction of fertilizer dose and variety had significant effect on all the plant characters except oil content of mustard (Brassica spp). The best result of the above characters was recorded in 100% recommended fertilizer dose in combination with BINA Sarisha-8. The next best result was obtained from 100% recommended fertilizer dose in combination with BINA Sarisha-5.

      PubDate: Sun, 17 Jul 2016 03:57:55 GMT
       
  • Impact of wastewater irrigation on major nutrient status in soil near
           Bhaluka industrial area of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Abdullah-Al-Zabir, M.W. Zaman, Md. Zakir Hossen, Md. Nizam Uddin, Md. Jamil Hossain Biswas and Abdulla-Al-Asif.
      The population increase has not only increased the fresh water demand but also increased the volume of wastewater generated. Treated or recycled wastewater (RWW) appears to be the only water resource that is increasing as other sources are dwindling. Increasing need for water has resulted in the emergence of domestic wastewater application for agriculture and its relative use. The present study was conducted at the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh during 2013 to evaluate the contribution of wastewater to major soil nutrients (N, P, K, S, Ca, Mg, B and Na) and fluctuation in physicochemical properties of soil (soil pH and Ec) from waste carrying canal at 10 selected sites of Bhaluka Upazila. Three (3) soil samples were collected at 0, 30 and 60 m distances from the waste discharging canals. The pH, EC, N, P, K, S, Ca, Mg, B and Na in soil samples decreased gradually with the increase of distance from waste discharging canal. Maximum concentrations of N at 60 and 0m distance varied from 8400 to 9700, P from 1850 to 5000, K from 4600 to 6000, S from 2000 to 4000, Ca from 7500 to 28800, Mg from 7500 to 7800, B from 90 to 2800 and Na from 2300 to 3100 μg g-1 in test soil.The results showed better nutrient status of the soil along waste discharge canals. The findings give applicable advice to commercial farmers and agricultural researchers for proper management and use of treated industrial wastewater for agricultural purpose.

      PubDate: Sun, 17 Jul 2016 03:47:17 GMT
       
  • Biochemical and phytochemical evaluation of Stevia rebaudiana

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Moniruzzaman Sohag Howlader, Sheikh Rashel Ahmed, Khadizatul Kubra and Md. Khairul Hassan Bhuiyan.
      The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.

      PubDate: Sun, 17 Jul 2016 03:44:54 GMT
       
  • Morphometric study of banded gourami (Colisa fasciata) in Jessore,
           Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sharmin Akter, Md. Farid Uz Zaman, Md. Hasan-Uj-Jaman, Iren Nahar Sithi, Dilruba Yesmin and Abdulla-Al-Asif.
      Population morphometry including population demography, size group distribution, length weight relationship and sex ratio of an important freshwater fish, Colisafasciata was studied which collected fromJessore and Jhenaidah districts in Bangladesh during May, 2014 to April, 2015. Random samples of 201 individuals (108males and 93 females) were collected from different fish markets in Jessore (Monirampur, Borobazar, Chowgasa) and Jhenaidah (Mohespur, Barobazar, Kotchandpur) districst. The mean size of the specimen was recorded 65.08 ±14.32 mm in length and 7.43 ±3.09 g in body weight. Females were found little smaller than males. The specimens were larger in winter than summer and monsoon season. But in monsoon season the females were larger than males. The maximum length of the specimen collected from Jhenaidah district was smaller compare to the specimen of Jessore district. Most of the population (29%) was observed under 55-64 mm size class. Linear relationship was obtained between the log length (mm) and log weight (g) of the species in respects of sexes, seasons, and districts. The allometric coefficient ‘b’ of the relationship indicated negative allometric growth of the species as ‘b’ value was less than 3. The overall sex ratio (male: female=1:0.86)showed significant difference from the expected value 1:1. Overall males showed non-significant over females (χ2 =1.119) in all season of both districts but in November males showed the significant (P

      PubDate: Sun, 17 Jul 2016 03:42:30 GMT
       
  • Prevalence of poultry diseases in Gazipur district of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Kamrul Hassan, Md. Humayun Kabir, Md. Abdullah-Al-Hasan, Shobnom Sultana, Md. Shohidul Islam Khokon and S. M. Lutful Kabir.
      This study was designed with a view to investigate the prevalence of poultry diseases in Gazipur district of Bangladesh. A total of 679 poultry birds (313 layers, 338 broilers and 28 cockrels) either dead or live were brought for diagnosis of diseases at Gazipur Sadar Upazilla Veterinary Hospital. The diseases were diagnosed on the basis of history, clinical signs and post-mortem findings. The diseases encountered in layers were bacterial diseases 52.29% (salmonellosis 38.56%, colibacillosis 6.7%, fowl cholera 4.79% and necrotic enteritis 1.60%), viral diseases 23.95% (avian influenza 2.56%, Newcastle disease 16.61%, infectious bronchitis 3.19% and avian leucosis 0.64%), mycoplasmal disease (mycoplasmosis 14.70%) and protozoal disease (coccidiosis 5.75%). Salmonellosis was most prevalent disease in age group of>20 weeks, while Newcastle disease most common in 8 to 20 weeks of age group. In case of broiler, bacterial diseases 28.99% (salmonellosis 21.30% and colibacillosis 7.69%), viral diseases 53.24% (infectious bursal disease 28.99%, Newcastle disease 8.87% and infectious bronchitis 15.38%), mycoplasmal disease (mycoplasmosis 7.1%) and protozoal disease (coccidiosis 6.5%). In cockrels, the most prevalent disease was colibacillosis 35.71% followed by salmonellosis 28.57%, Newcastle disease 14.28% and mycoplasmosis14.28%. So among the diseases, salmonellosis is most prevalent disease followed by infectious bursal disease and mycoplasmosis in different kinds of poultry of Gazipur district of Bangladesh.

      PubDate: Sun, 17 Jul 2016 03:39:47 GMT
       
  • Epidemic behavior of the etiological agent of infectious coryza in layer
           chicken of Bangladesh with isolation, identification and pathogenicity
           study

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mir Rowshan Akter, Md. Shahidur Rahman Khan, Md. Mostafizer Rahman, S. M. Lutful Kabir and Md. Abu Sayed Khan.
      The present study was selected as infectious coryza is one of the major problems affecting poultry industry in the developing country like Bangladesh and the reports regarding infectious coryza are yet not be documented considering epidemiological investigation, proper isolation, identification and pathogenicity study. The epidemic behavior of the etiological agent of this disease were studied based on age, sex, breed, spatial and temporal differences after collection of samples suspected to be infected with infectious coryza in layer chicken of Bangladesh. The incidence rate of infectious coryza from field cases were recorded as per information received from farmers by using a structured questionnaire and also clinical signs and symptoms. The disease was very high in laying hen (18.38%) in Sylhet and growing birds (7.25%) in Khulna in comparing with prelaying stage (2.07%) also in Sylhet region of Bangladesh. In this study no significant differences was observed as their location variation except Sylhet (9.2%) in comparison with other areas (Rangpur – 8.76%, Rajshahi -8.82%, Khulna – 8.83%, Dhaka – 8.72 and Chittagong – 8.65% respectively) of Bangladesh but significant differences was observed as their age group. However, the incidence rate of this disease was found to be very high during winter (8.77%) in compare with summer (0.42%) season. Moreover, during investigation a total of 122 samples were collected from different areas of Bangladesh for the period of March 2011 to February 2014. The higher rate of incidence of A. paragallinarum was found in Sylhet (66.66%) and lowest in Dhaka (43.75%). The association of A. paragallinarum with different seasons revealed that higher incidence rate was found in winter season (52.26%) in comparison with summer season (1.85%). The suspected positive isolates were subjected to experimental pathogenicity study in natural host for there – isolation of A. paragallinarum was done as per Kotch postulates.

      PubDate: Sun, 17 Jul 2016 03:24:07 GMT
       
  • Mitigation of salt stress in rice plant at germination stage by using
           methyl jasmonate

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sakil Mahmud, Shayla Sharmin, Bishan Lal Das Chowdhury, Mohammad Anowar Hossain and Muhammad Javidul Haque Bhuiyan.
      To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 μM MeJA and saline (12 dSm-1) + 20 μM MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 μM had a better effect over 20 μM MeJA.

      PubDate: Sun, 17 Jul 2016 03:21:34 GMT
       
  • Socio-economic condition of fishermen and intermediaries involved in
           marine fish marketing chain in Cox’s Bazar area, Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Khabirul Ahsan, Nusrat Sultana Runa, Subrata Kumar Ghosh, Muhammad Mehedi Hasan and Md. Kamal.
      Study was conducted to know the socio-economic condition of the fishermen and intermediaries of fish marketing chain in Cox’s Bazar area, Bangladesh. Socio-economic condition of the fishermen and intermediaries were not so good in the study area. It was found that about 35% fishermen and intermediaries were illiterate, 16% fishermen and intermediaries were semiliterate who only could write their names. 55% household of the fishermen and intermediaries had tin shed house with tin wall. Average income for most of fishermen and intermediaries was about Tk. 400-500 and 66% were found to borrow money from different source for continuing their business. Poor road and transport facilities, lack of credit facilities, insufficient supply of ice, inadequate training facilities and large number of intermediaries in the chain were the main problems of the marine fish marketing chain. It is therefore necessary to provide institutional and organizational support, government support, extension service and training facilities to the market operators for sustainable fish marketing systems.

      PubDate: Sun, 17 Jul 2016 03:19:41 GMT
       
  • A surveillance study on dairy farmers selected in Baghabari milk pocket
           area of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Nuruzzaman Munsi, Md. Azharul Islam Talukder, Md. Zillur Rahman and Md. Abu Hemayet.
      This study was carried out to reveal the animal agriculture status of the dairy farmers. The survey was conducted by random sampling on 100 dairy farmers (50 from Shahzadpur upazila and 50 from Bera upazila). A pretested questionnaire was developed and multiplied for data collection. The data collected were brought to the headquarters of Bangladesh Livestock Research Institute, Savar, Dhaka for analysis. After analysis it was found that the education level of the farmers of Shahzadpur upazila was better than that of Bera upazila, where 74% farmers of Shahzadpur and 66% farmers of Bera were engaged in agriculture. The average annual income and expense of the farmers of Shahzadpur were greater than that of Bera. The average total land, cultivable land and total livestock population per farmer were also higher in Shahzadpur upazila than in Bera upazila. In case of diversity of different genotypes of dairy cattle, except Jersey cross the number of Pabna, Sindhi cross, Shahiwal cross and H-F cross was much greater in Shahzadpur than in Bera. Most of the farmers purchase concentrate feeds and produce roughages in both the locations, where feed scarcity was seen mostly during rainy season. About 56% farmers treat their cattle with the help of veterinary surgeon and 44% farmers provide treatment with the help of veterinary field assistant in Shahzadpur, while the scenery was almost opposite in Bera. About 90% farmers of Bera upazila and 95% of Shahzadpur upazila used anthelminitics to deworm their animals. Most of the farmers in both sites dewormed their cattle every three months. About 78% farmers of Shahzadpur and 76% of Bera vaccinated their animals. About 85% farmers of Shahzadpur had been found to properly dispose the carcasses, where as only 48% farmers had been found to do it in Bera. Almost all the farmers washed their hands, equipment and udders of the cows with clean water before milking in both Shahzadpur and Bera. Approximately, all the farmers of both the locations milked their cows twice a day, did not perform milk testing and sold the raw fresh milk to the cooperative societies. All farmers used cow dung as fuel. Overall, it was revealed that Shahzadpur upazila was more advanced than Bera upazila in almost all aspects of animal agriculture.

      PubDate: Sun, 17 Jul 2016 03:16:52 GMT
       
  • Effects of saline water on health status of pregnant women in coastal
           regions of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Zohirul Islam Rony, Md. Shafiqul Islam Khan, Md. Ali Asgar, Musammet Rasheda Begum and ASM Golam Kibria.
      Drinking water from natural sources in coastal region of Bangladesh has been contaminated by varying degrees of salinity which have detrimental effects on the health. Pregnancy itself is a crucial time for women and the salinity increases its vulnerability regarding the raising of blood pressure. The study was conducted to evaluate the effect of saline water intake on the health status of pregnant women at Barguna and Patuakhali districts, Bangladesh. Sodium (Na) concentration in urine samples was analyzed and corresponding blood pressure was taken in the diagnostic laboratory. Descriptive statistical analysis showed that Na concentration, systolic and diastolic pressure (BP) was the highest in 2nd trimester followed by 3rd trimester and 1st trimester. Water intake and urine production were the highest in 3rd trimester and increased with age. Na concentration, systolic and diastolic pressure (bp) was the highest in 3rd parity followed by 2nd parity. Water intake and urine production did not very among parities. Correlation matrix express that sodium concentration has the significant association with the age and the highly significant association with systolic and diastolic blood pressure. In Logistic Regression Model, odds ratio (OR) estimate showed that women with systolic bp≥120 had 1.109 times greater sodium concentration than with systolic blood pressure of

      PubDate: Sun, 17 Jul 2016 03:13:45 GMT
       
  • Poverty alleviation of destitute women and poor farmers in Barisal
           district of Bangladesh through beef fattening

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mohammad Rohul Amin, Mohammad Hafizur Rahman, Milton Talukder, Md. Ali Asgar, Ashit Kumar Paul and Mohammad Lalmoddin Mollah.
      The cattle fattening programme was represents an important component of the agribusiness sector with great economic, income, poverty alleviation and social implications. The present study was performed at Barisal district in Bangladesh. Data were collected from 220 women and farmers on their household during the period June, 2013. Among them, 54 destitute women and poor farmers were identified and making groups of them for beef fattening. 54 cattle of each destitute women and poor farmers were randomly divided into nine groups (A, B, C, D, E, F, G, H and I), each consisting of six (6) cattle. Endex®, Aldazole® and Tetranid® were administered orally to the cattle of group A, B and C, respectively. Cattle of group D received subcutaneously Oxynil®. Cattle of group E received orally Peraclear® and Urea Molasses Straw (UMS). Cattle of group F received orally Endex® and lugols iodine intravenously. Cattle of group G were treated orally with Endex® and Metaphos® intramuscularly. Cattle of group H were treated orally with Endex® and A-Sol® intramuscularly. Cattle of group I was kept as control without giving any treatment. Body weight was examined before trials (day 0) and on 10th day, 20th day, 30th day, 40th day, 50th day, 60th day and 70th day. Out of the 220 women and farmers 56.36% were involved in agriculture, 10.45% in own business, 3.64% in shared business, 6.36% in govt. service and 23.18% in non-govt. service. 18.18% women and farmers had the knowledge of animals characteristics used for beef fattening. The knowledge of feed, animal diseases, veterinary drugs, animal vaccine, and drugs used for beef fattening was 18.64%, 47.73%, 25.45%, 25.45%, and 19.55%, respectively. About 7.73% respondents have training on cattle fattening. The body weight was increased significantly (p

      PubDate: Sun, 17 Jul 2016 03:11:22 GMT
       
  • Effect of arsenic trioxide along with tannic acid, di-sodium hydrogen
           phosphate, alum and effects of sand-charcoal-iron-filter bed filtrated
           water along with alum on body weight and some hematological parameters in
           rabbit

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Shaifuddin Ahmed, Md. Saiful Islam Siddiqui, Kamrul Islam, Mohammad Usman Gani, Sharifunnessa Moonmoon, Md. Harun-or-Rashid and Md. Abdul Awal.
      The effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and effects of sand-charcoal-iron-filter (SCIF)-bed filtrated water along with alum on body weight and some hematological parameters were carried out on a total of 30 (01 month old) adult Newzealand white rabbits. The Rabbits were randomly divided in to 6 equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100 ppm, group C received arsenic trioxide @ 100 ppm plus tannic acid @100 ppm, group D received arsenic trioxide @ 100 ppm plus di-sodium hydrogen phosphate @100 ppm, group E received arsenic trioxide @100 ppm plus alum @100 ppm orally daily for 60 days in all cases and group F received alum @100 ppm in SCIF-bed filtrated water orally daily for 60 days. The result showed that body weight gain of control group (A) per week per rabbit was found to increase but in treated group B (arsenic trioxide@100ppm orally daily) the mean body weight of group B were decreased gradually and significant (P

      PubDate: Sun, 17 Jul 2016 03:07:26 GMT
       
  • Sero-surveillance and sero-monitoring of locally produced PPR vaccine in
           the field and experimental level

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Ehsanul Kabir, Md. Mokbul Hossain, Md. Ershaduzzaman, Md. Abu Yousuf and Md. Rafiqul Islam.
      Peste des Petits Ruminants (PPR) is a highly contagious, economically important viral disease of goats with high morbidity and mortality. To control the disease effectively a live attenuated vaccine is available in Bangladesh which is produced by Livestock Research Institute (LRI), Mohakhali, Dhaka. The study was carried out to determine the immune status and immune response against PPR in field and experimental Black Bengal goats. Sero-surveillance of PPR was conducted by using c-ELISA in non-vaccinated 240 goats in Gazipur, Sirajgonj and Barisal. Out of the 240 goats tested, of which only 39 (20.31%) goats had positive level of PPR antibodies while 16.25% (13 out of 80 goats) in Gazipur, 28.75% (23 out of 80 goats) in Barisal and 3.75% ((3 out of 80 goats)) in Sirajgonj. In case of sero-monitoring of PPR, the result revealed that vaccinated goats from Rajshahi showed high positive result and have higher seroprevalence where 75% (60 out of 80 goats) were seropositive and only 25% (20 out of 80 goats) are seronegative. These result indicated that vaccinated Rajshahi goats is more resistant for PPR virus than non vaccinated goats. In experimentally to perform sero-monitoring, 10 seronegative goats were selected and divided into two equal groups (A and B).The immunization study against PPR with a commercial PPR vaccine was conducted on 5 goats of group A by inoculating @ 1.0 ml vaccine / animal subcutaneously and group B kept as non-vaccinated. The antibody titres against PPR in goats were determined at 0 day on vaccination and after 21DPV, 180DPV and 365DPV. The results found that 100% (5 out 5goats) seronegative in both vaccinated goats of group A and non-vaccinated goats of group B at 0 day on vaccination. The mean negative titres± SD were 79.285±13.921 and 76.707±9.265 in vaccinated group A and group B, respectively. The mean positive titers ±SD were 20.201±2.480, 8.630±4.970 and 11.382±1.419 at 21DPV, 180DPV an 365DPV, respectively in group A (100% seropositive). In case of non-vaccinated group B, the mean negative titres±SD were 74.258±7.793, 77.726±9.142 and 82.965±7.492 at 21DPV, 180DPV and 365DPV, respectively (100% seronegative). As it is observed, the antibody titres remain at the level over the period of time that indicates the immune response against PPR. From this finding, it is said that PPR vaccine could produce immune response in goats for about one year or 365 days.

      PubDate: Sun, 17 Jul 2016 03:04:22 GMT
       
  • Identification of genera of tubificid worms in Bangladesh through
           morphological study

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mariom, Sharmin Nahar Liza and Md. Fazlul Awal Mollah.
      Tubificids are aquatic oligochaete worms (F- Naididae, O- Haplotaxida, P- Annelida) distributed all over the world. The worms are very important as they are used as live food for fish and other aquatic invertebrates. A step was taken to identify the genera of tubicifid worms that exist in Mymensingh district, Bangladesh on the basis of some external features including the shape of their anterior (prostomium) and posterior end, number of body segment and arrangement of setae. The study result indicated the existence of three genera among the tubificid worms. These were Tubifex, Limnodrilus and Aulodrilus. All these three genera possessed a cylindrical body with a bilateral symmetry formed by a series of metameres. The number of body segments ranged from 34 to 120 in Tubifex, 50 to 87 in Limnodrilus, and 35 to 100 in Aulodrilus. In Tubifex, the first segment, with the prostomium, was round or triangular bearing appendages, whereas, in Limnodrilus and Aulodrilus, the prostomium without appendages was triangular and conical, respectively. Three types of setae i.e., hair setae, pectinate setae and bifid setae of various sizes were found in Tubifex, whereas, Limnodrilus possessed pectinate setae and bifid setae of more or less similar size and shape; and Aulodrilus possessed only hair setae and bifid setae of different size.

      PubDate: Sun, 17 Jul 2016 03:01:34 GMT
       
  • Evaluation of nutritive value and shelf life of strawberry genotypes

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sushan Chowhan, Md. Shyduzzaman Roni, Shaikh Shakib Ahmed, Hanif Uddin and Md. Mahmudul Hasan.
      An investigation was carried out to evaluate the nutritive value and shelf life of five strawberry genotypes. It was found that under fresh condition the highest ascorbic acid (45.76 mg/100g) was obtained by Rabi-3 and the highest -carotene (15.09 IU/100g) by Festival. The maximum amount of reducing sugar (6.373 g/100g) and non-reducing sugar (2.947 g/100g) was recorded in Camarosa and Festival respectively. The maximum amount of calcium (0.480%) in BADC Strawberry, iron (93.50 ppm) in Rabi-3, phosphorus (0.081%) and potassium (0.854%) in BARI Strawberry-1 was recorded. Under different temperature and packaging conditions, polyethylene package at 40C proved to be superior to others in all respects. The highest shelf life (47.00 days) and ascorbic acid (42.76 mg/100g) after four days of storage was obtained by Camarosa in this condition; whereas the lowest shelf life (2.33 days) and ascorbic acid (10.12 mg/100g) was recorded in BADC Strawberry in plastic punnet package at 250C.

      PubDate: Sun, 17 Jul 2016 02:58:58 GMT
       
  • Seroprevalence of ovine brucellosis in Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Osman Gani, Md. Nuruzzaman Munsi, Md. Ershaduzzaman, A.K.M. Anisur Rahman, Sajeda Sultana and Md. Shahin Alam.
      An investigation was carried out to study the seroprevalence and risk factors for Brucella seropositivity in sheep in Bangladesh. For this, highly sheep populated ten different districts including all seven divisions of Bangladesh were selected where sheep of Panchagarh (151), Naogaon (101), Tangail (83), Noakhali (56), Sunamganj (52), Patuakhali (119) and Dhaka (75) were tested but sheep of Chapai Nawabganj, Khulna, Noakhali and Feni has under tested for Brucellosis. In the present study, seroprevalence of ovine brucellosis was tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme-Linked Immunosorbent Assay (ELISA). About 5% sheep might have got Brucella infection. The highest Brucella antibody was observed in sheep of Tangail district (8.4%) followed by the sheep of Savar (8.0%), where as sheep of Subarna Char and Kala Para were free from Brucella antibody. In relation to age and sex, adults (6.5%) were more positive than young (3.6%) and female (5.3%) were more susceptible than male (3.8%).

      PubDate: Sun, 17 Jul 2016 02:56:59 GMT
       
  • Feeding and breeding management system of goat at central and southern
           regions of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Panir Choudhury, Anguara Khatun, Md. Shamsul Hossain and Farukul Islam.
      Data were collected to study the feeding and breeding management system of goat from the villages of Mymensingh, Barguna and Patuakhali districts in Bangladesh through direct interviewing method using a pre-structured questionnaire. The research work was conducted from June to July 2014. Most of the goat rearing farmers in central and southern region of Bangladesh reared goats in semi-intensive system and kept their goats at goat’s house. Farmers took winter care and took bath of their goats during summer. Maximum farmers (82%) of central region supplied concentrate feed but 61% farmers of southern region did not supply concentrate to their goat. Farmers (82%) of central region provided particular feeder but 67.7% farmers of southern region did not provide feeder during concentrate feeding. Tube well (84%) was the main source of water of goat at central part of Bangladesh. But main sources of water in southern region were (46.3%) tube well and (51.2%) pond. All farmers of central region did not keep buck but only 19.5% farmers of southern region kept buck for breeding their female goat. Ninety two percent famers of central part of Bangladesh and 70.7% farmers of southern region used village buck for breeding their does. All buck keeping farmers at central region received service charge during breeding and they also practiced close breeding method. Most of (73.2%) the buck keeping farmers at southern region of Bangladesh received service charge of buck and they did not practice close breeding system. So, the present study might be suggested that farmers at central region were more careful of their goats in terms of feeds, feeding, water sources selection and watering but farmers at southern region were more aware about buck keeping and inbreeding depressions in their goats.

      PubDate: Sun, 17 Jul 2016 02:54:20 GMT
       
  • Omega-3 fatty acids transport through the placenta

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Ariful Islam, Takanori Kodama, Yui Yamamoto, Majid Ebrahimi, Hirofumi Miyazaki, Yuki Yasumoto, Yoshiteru Kagawa, Tomoo Sawada, Yuji Owada and Nobuko Tokuda.
      The placenta is a temporary vital organ for sustaining the development of the fetus throughout gestation. Although the fatty acid composition delivered to the fetus is largely determined by maternal circulating levels, the placenta preferentially transfers physiologically important long-chain polyunsaturated fatty acids (LC-PUFAs), particularly omega-3 (n-3) FAs. The precise mechanisms governing these transfers were covered in a veil, but have started to be revealed gradually. Several evidences suggest fatty acid transport proteins (FATPs), placental specific membrane bound fatty acid binding proteins (pFABPpm) and fatty acid translocases (FAT/CD36) involved in LC-PUFAs uptake. Our studies have shown that the placental transfer of omega-3 FAs through the trophoblast cells is largely contributed by fatty acid binding protein 3 (FABP3). Recently there are considerable interests in the potential for dietary omega-3 FAs as a therapeutic intervention for fetal disorders. In fact, prenatal supply of omega-3 FAs is essential for brain and retinal development. Recent findings suggest a potential opportunity of omega-3 FA interventions to decrease the incidence of type 2 diabetes in future generations. In this review, we discuss the molecular mechanism of transportation of omega-3 FAs through the placenta and how omega-3 FAs deficiency/supplementation impact on fetal development.

      PubDate: Sun, 17 Jul 2016 02:51:48 GMT
       
  • Effect of different types of organic manure on biomass yield,
           morphological characteristic’s and nutritive value of BLRI Napier-4
           in Baghabari milk shed area, Sirajgonj

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Zillur Rahman, Md.Yousuf Ali, Nathu Ram Sarker, Most. Farhana Afroz, Md. Obayed Al. Rahman, Md. Rezaul Karim and Md. Azharul Islam Talukder.
      The trial was conducted at Bangladesh Livestock Research Institute (BLRI) regional station, Baghabari, Shajadpur, Sirajgonj to determine the biomass yield, morphological characteristics and nutritive value with the effect of different sources of organic manure such as biogas slurry, broiler litter and layer litter for fodder production. Significant (P fertilizer (26.56)> broiler litter (22.34)>layer litter (22.20). This study also showed that DM yield and survival percentage were also significantly (P≤0.05) differed among the treatments. The DM% in all groups was mostly similar but DM yield (t/ha) was the highest (3.94) in biogas slurry and the lowest (3.13) in layer litter. The cost (Tk/ha) was significant (P0.01) effect of different organic manures on nutritive value of BLRI Napier-4. It may be suggested that biogas slurry may be used as sources of fertilizer for fodder cultivation.

      PubDate: Sat, 16 Jul 2016 05:23:09 GMT
       
  • Anti-fungal potential of tridhara (Tridax procumbens) leaves

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Faruk Miya, Zakirul Islam, Sayeed Shahriyar, Md. Riad Hossain Khan and Md. Salim Reza.
      The antifungal activity of methanolic extracts of leaves of Tridax procumbens was studied. Fungal strains Aspergillus niger and Aspergillus ocraceous were selected for the study. Disc diffusion method was applied against the selected fungal strains and compared to the drug ciprofloxacin to observe the antifungal activity of the methanolic extracts of Tridax procumbens leaves. The present study demonstrated that the disks of methanolic extracts of T. procumbens showed effective inhibition against A. niger and A. ocraceous compared to ciprofloxacin. The results of the current investigation suggest that the methanolic extracts of T. procumbens leaves can be used for the treatment of the diseases caused by the tested organism and it has significant scope to develop a novel broad spectrum of antifungal herbal formulations.

      PubDate: Sat, 16 Jul 2016 05:20:29 GMT
       
  • Isolation, identification and characterization of bacterial flora from the
           respiratory tract of apparently healthy sheep

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Chandan Kumar Sarker, Anowar Hossen, Md. Abu Yousuf, Md. Ala Uddin, Most. Sumona Akter, Mostafizur Rahman and Md. Bahanur Rahman.
      Sheep is the common name for a group of grazing mammals that may be either wild or domesticated; the domesticated varieties are amongst the most widely distributed types of domestic animal, found in nearly all countries. Bangladesh is a densely populated developing country and its economy is primarily based on agriculture. The current study was designed for isolation, identification and characterization of bacterial flora from the upper respiratory tract of sheep. Thirty (30) apparently healthy sheep were selected at the adjacent areas of Bangladesh Agricultural University (BAU) for this experiment. Swab samples were collected from nasal swabs (10), lung swabs (10) and tracheal swabs (10). All samples were subjected into inoculated on to bacteriological media (nutrient broth, nutrient agar, Salmonella-Shigella agar, MacConkey agar, blood agar, brilliant green agar). Furthermore, all of the bacterial isolates were characterized by Gram’s staining, biochemical tests (sugar fermentation tests, catalase test, coagulase test, indole test, MR-VP test), antibiotics sensitivity tests and pathogenicity tests. None of the isolated Bacillus, E. coli and Staphylococcus spp. was found to be pathogenic. Isolated Pasteurella spp. were found to be pathogenic as observed in different experimental models and showed a degree of variation in antibiotic drug sensitivity test. Ciprofloxacillin was sensitive to all of the isolated bacteria. Through the bacteria that were isolated from various organs of apparently healthy sheep is normal micro flora, however these may act as primary pathogen and may produce diseases when the sheep are immunologically suppressed due to severe stress conditions.

      PubDate: Sat, 16 Jul 2016 05:17:44 GMT
       
  • Bio-security in small scale poultry farms against avian influenza:
           knowledge, attitude and practices

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Nelima Ibrahim, Marufa Akhter, Selim Al Mamun, Emdadul Haque Chowdhury and Priya Mohan Das.
      Avian influenza (AI) is considered as one of the greatest global threat for the poultry industry that the animal health sector has ever had to face. It is primarily an infectious disease of birds caused by influenza virus Type A strain. The major concern now is that a highly pathogenic strain (H5N1) has also been shown to transmit to humans and has the potential to be fatal. Since March 2007, outbreaks of highly pathogenic avian influenza (HPAI) have been occurring in commercial and backyard poultry in Bangladesh. Good bio-security practices can help reducing the risk of spreading and controlling the disease. This investigation describes the bio-security practices of small scale poultry holders (500-2000 birds/ farm) of Gazipur district, their knowledge and attitude in prevention and control of avian influenza. This was assessed using prescribed questionnaire. This study has been conducted on 100 poultry raising farmers through household-based individual interviews. Though respondents had different opinions on the magnitude of AI in their respective area, almost everyone realized AI is a big problem for Bangladesh. Generally, the respondents were not aware of the common infection sources such as, sick poultry, their pens, cages, backyard poultry, wild animals, migratory birds etc. Most of the interviewed small scale farmers in this area were not aware about the strict bio-security process like segregation of diseased birds, cleaning and disinfection of premises to prevent AI. Although there was a basic knowledge about the dangers and economic consequences of AI, there needs to be an updating of information on sources of infection, symptoms and prevention techniques, as well as an understanding of the cross species dangers of the infection. The study has, to a large extent, successfully drawn up a picture of how Bangladeshi small holder farmers have perceived and responded to AI and what they have understood and what practices they are taking against AI in their respective areas.

      PubDate: Sat, 16 Jul 2016 05:14:49 GMT
       
  • Flowering and fruiting behavior of velvet apple

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Tanvir Hossain, Md. Saiful Islam, Md. Fakhrul Hasan, Subrata Mojumder, Mahbub Robbani, S.M. Ahsan and Debasish Mondal.
      The study was conducted at three upazilla of Patuakhali during the period from the 1st February, 2013 to the 31st July, 2013 to evaluate the flowering and fruiting behavior of velvet apple germplasm. Twelve velvet apple germplasm were selected from three locations (DD 01, DD 04, DD 06, and DD 09 from Dumki; DD 02, DD 05, DD 08 and DD 10 from Mirzaganj; DD 03, DD 07, DD 11 and DD 12 from Patuakhali sadar upazilla) by field survey. The age of plant varied from 10 to 25 years having satisfactory growth and good health. A lot of variations in different parameters were observed among the germplasm. Flowering season started from 22 February and continued up to 20 April. Percentage of fruit setting ranged from 65.15 % to 92.26 % in DD 07 and DD 01, respectively. Required time for fruit maturation ranged from 116 days (DD 01) to 129 days (DD 07). Single fruit weight varied from 198.10 g (DD 12) to 238.90 g (DD 01). The highest number of seeds (8) was found in DD 07 while DD 01 was seedless. The length and diameter of fruit ranged from 6.72 cm (DD 07) to 8.04 cm (DD 01) and 6.09 cm (DD 07) to 7.27 cm (DD 01), respectively. The maximum edible portion (87.33 %) was recorded in seedless germplasm DD 01, while it was the lowest (42.00 %) in DD 07.

      PubDate: Sat, 16 Jul 2016 05:12:10 GMT
       
  • Early emergence of axons and formation of accessory nerve (XI) in avian
           embryos

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Ziaul Haque, Azimul Haque, Qin Pu and Ruijin Huang.
      Development of the nervous system involves specification of distinct classes of neurons at defined locations within the central nervous system (CNS). In the head–trunk-transitory region accessory nerve (XI) display a unique axonal trajectory in that they ascend along the lateral margin of the spinal cord and the medulla oblongata. At present, we lack a detailed description of early emergence and development of the accessory nerve (XI) in avian embryos. To know the early projection of accessory axons from the central nervous system (CNS) and formation of accessory nerve (XI), whole-mount immunostaining of chick embryos was performed. Our results showed that axons start to project from the CNS as early as Hamilton and Hamburger (HH) stage 18 at the level of occipital somites (1 to 5). In the succeeding developmental stages, the axons developed, united with each other and ran dorsolaterally along the longitudinal axis of the embryo. Finally, it formed a bent near the first occipital somite and passed along with vagus nerve. This study will give us an idea on the topographic anatomy of accessory nerve (XI) during development of chick embryos.

      PubDate: Sat, 16 Jul 2016 05:09:56 GMT
       
  • Effect of feeding tree forages on productive performances on growing sheep

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Zillur Rahman, Md.Yousuf Ali, Md. Azharul Islam Talukder, Md. Ershaduzzaman and Most. Sumona Akter.
      A research work was undertaken to evaluate the feeding effect of tree forages on performance of growing sheep. Twenty growing sheep (in 4 groups) were fed three different tree forage diets. Tree forages Melia azardirachta, Leucaena leucocephala and Artocarpus heterophyllus were supplied in three treatments except one consisted no tree forages which was considered as control. There were significant (p

      PubDate: Sat, 16 Jul 2016 04:34:04 GMT
       
  • Effect of cypermethrin on the histoarchitecture of gills and liver of a
           freshwater catfish, Pangasianodon hypophthalmus

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Shirajum Monir, Mohammad Ashaf-Ud-Doulah, Md. Khalilur Rahman, Jubaida Nasreen Akhter and Md. Rayhan Hossain.
      Histoarchitecture effect of cypermethrin was investigated in the gills and liver of freshwater catfish, Pangasianodon hypophthalmus (weight 60-70g) over an exposure period of 96 h as an endpoint of toxicity. The fish were exposed to five acute concentrations (0.00ml/L, 0.025ml/L, 0.050ml/L, 0.075ml/L and 0.10ml/L) and the 96 h LC50 was 0.075 ml/L. The physical reactions observed in the treated fish were erratic swimming, discolorations of the skin, loss of reflex, hyperactivities, surfacing, increasing opercula ventilation and these effects increased with increasing concentration of the toxicants and duration of exposure. The most common changes in gills histoarchitecture at all doses of cypermethrin were curl shape gill lamellae, bulged with the fusion of secondary gill lamellae, severe epithelial necrosis in gill lamellae and hypertrophy. The changes observed in the liver tissues were cloudy swelling, focal necrosis and hypertrophy of hepatocytes, degeneration of hepatocytes and cytoplasmic, extensive vacuolation of hepatocytes and pyknotic nuclei. The results of this histoarchitecture analysis of gills and liver tissues indicate a direct correlation between cypermethrin exposure and the histoarchitecture disorders.

      PubDate: Sat, 16 Jul 2016 04:32:15 GMT
       
  • Livestock and poultry health management in Saint Martin’s island

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Uday Kumar Mohanta, Md. Nuruzzaman Munsi, Md. Azharul Islam Talukder, Emdadul Haque Chowdhury and Md. Mafijul Islam.
      The study was performed to find out the existing status of livestock and poultry in the Saint Martin’s island, to determine the prevalence of parasitic and other infectious and non-infectious diseases of these, and to find out the existence of intermediate hosts of various parasites in the island. Prevalence of endoparasites in cattle, buffalo and goat was higher in winter than that of summer. Prevalence of stomach worm was highest in goat in both winter (50%) and summer (40%). 31.47% cattle harbored stomach worm infection in winter and 15.79% in summer. More than 20% buffaloes suffered from either single infection with Fasciola and Paramphistomum or in combination in both the seasons. Goat suffered from some extra species like Moniezia and Trichuris and the prevalence is just double in winter than that of summer. Morbidity rate of FMD in cattle is high, although mortality is low. But, in case of chicken, both morbidity and mortality rates were high enough to make a massive loss with respect to number and production due to Ranikhet/Newcastle Disease (ND), pox in the island. About 92.11% of total chicken population was lost every year due to outbreak of ND. Incidence of infectious disease in livestock and poultry in the island is very high as because the farmers cannot immunize the animals through routine vaccination. Even some farmers do not know about the immunization of livestock and poultry, and the veterinary service is not available in the island.

      PubDate: Sat, 16 Jul 2016 04:21:40 GMT
       
  • Detection of avian reovirus antibodies in layer birds of small scale
           commercial farms in Dinajpur district of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Abdus Salam, Md. Atiqul Haque, Md. Mostafizer Rahman, Mir Rowshan Akter and Farzana Afroz.
      The present study was conducted on layer birds of different age groups to determine specific antibody titer level against avian reovirus (ARV) by indirect enzyme linked immunosorbent assay (iELISA) at Dinajpur district of Bangladesh. This study showed that ARV specific antibody positive cases were 84 out of 90 blood serum samples and the highest antibody titer was 26120 and lowest antibody titer was 288. The total 93.33% sera samples were showed positive result. The study showed that 100% sera sample were positive against ARV at 6 weeks of aged group and the highest, lowest and mean antibody titer were 13917, 4895 and 10269 respectively. On the other hand 88.88% sera sample were positive against ARV at 10 weeks of aged group and the highest, lowest and mean antibody titer were 9779, 288 and 5689.89 respectively. The sera sample collected from 14 weeks of aged group showed 88.88% positive and the highest, lowest and mean antibody titer were 11727, 871 and 5250 respectively. The sera sample collected from 18 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 24440, 1234 and 12648.89 respectively. The sera sample collected from 22 weeks of aged group were 100% positive against ARV and the highest, lowest and mean antibody titer were 26120, 1752 and 11373.89 respectively. The sera sample collected from 26 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 8566, 1630 and 4327.44 respectively. The sera sample collected from 30 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 13431, 1989 and 5890.56 respectively. The sera sample collected from 40 weeks of aged group showed 77.77% positive against ARV and the highest, lowest and mean antibody titer were 14618, 433 and 5103.22 respectively. The sera sample collected from 48 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 14553, 957 and 7436.5 respectively. In conclusion it is evident that avian reovirus-specific antibody was successfully detected through commercially available avian reovirus antibody test kit (ELISA kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely ARV.

      PubDate: Sat, 16 Jul 2016 04:16:00 GMT
       
  • Induced breeding of Vietnames koi (Anabas testudineus) using pituitary
           gland (PG) and ovaprim at Tasty Fish and Feeds Industries Limited,
           Melandah, Jamalpur

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Ruhul Amin, Md. Mamunur Rahman, Sopna Aktar, Md. Badrul Alam and Md. Ashraful Alam.
      The research work was conducted for induced breeding of Vietnames Koi (Anabas testudineus) using carp PG and Ovaprim hormone at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur during March to May, 2015. Brood fishes were reared for 4-5 months in the brood rearing pond by providing farn maid artificial feed (containing 32% protein) for maturation before onset of breeding season. Two different hormones as inducing agents PG-PG, and PG-Ovaprim were used for the purpose of A. testudineus breeding in the months of March to May, 2015. In the present experiment, the broods were induced with PG-PG hormone (2 mg PG/ Kg body weight for male and 7 mg PG/Kg body weight for female) and PG-Ovaprim hormone (2 mg PG/Kg for male and 7 ml Ovaprim/Kg for female) were designated as treatments T1 and T2 respectively. Ovulation rates 100%, fertilization rates were 78.11 ± 3.24% and 71.00 ± 3.97%, hatching rates were 70.22 ± 3.40% and 60.89 ± 4.20% and survival rates were 51.22 ± 3.96% and 48.89 ± 4.41% in T1 and T2 respectively. The highest fertilization rate (78.11%), hatching rate (70.22%) and survival rate (51.22%) were found in the T1. Comparing the PG-PG and PG-Ovaprim doses, the highest breeding performance was observed in PG-PG dose.

      PubDate: Sat, 16 Jul 2016 04:13:55 GMT
       
  • Impact of aqua drugs and chemicals on the recoveries of fish diseases and
           total fish production in Sherpur region of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Gias Uddin Ahmed, Mohammad Nurul Alam and Md. Mamunur Rahman.
      The present study was conducted to investigate the impact of aqua-drugs and chemicals on fish health and total production in Sherpur region, Bangladesh from July 2014 to June 2015. Forty nine different types of aqua-drugs and chemicals were recorded in the study area, among those sixteen types mainly ADDIC drugs and chemicals were widely used by the farmers for different fish disease treatment. It was recorded that GR Plus, Active Blue with Copper sulphate, ID Plus, Aqua c vit and H. vit plus were used for the treatment of EUS in Pangus, Shing and Thai koi which had an average recovery of 80-90%. For the treatment of Edwardsiellosis in Pangus and Thai koi, farmers used GR Plus, ID Plus, OTC power, Aqua c vit, H.vit plus and potassium permanganate which had an average recovery of 75-80%, and for the treatment of dropsy in Thai koi and Shing GR Plus, ID Plus, OTC power, Aqua c vit, H.vit plus and potassium permanganate were used which had an average of 75-85% recovery. Histopathology of liver and kidney of fishes were almost normal in control ponds, whereas, in drugs treated pond of fishes organs had pathological changes like necrosis, pyknotic cells, hemorrhage, fat droplet, ilets of langerhens, degeneration of kidney tubules and vacuums. However, total production of Thai koi, Shing and Pangus was 10,000 kg/acre, 10,000 kg/acre and 13,000 kg/acre in drugs treated ponds respectively. Whereas, it was 5,000 kg/acre, 4,500 kg/acre and 6,500 kg/acre in non-treaded ponds respectively in both upazillas. In control ponds, mortality rate comparatively high and production also low than drugs treated ponds. End of the experiment, it was observed that efficacy of drugs of Aqua Drug and Disease Information Centre (ADDIC) was excellent.

      PubDate: Sat, 16 Jul 2016 04:11:13 GMT
       
  • In vitro regeneration protocol development via callus formation from stem
           explant of tomato

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Meherunnesa Papry, S.M. Ahsan and Sayeed Shahriyar.
      The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (21.00) of roots/plantlet, length (7.676 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the stem explants. The results of the current study showed significant increase in the growth of callus of Solanumlycopersicon Mill. Indicating a good efficiency of the optimized media composition and the experimental model used in comparison to other studies of similar nature.

      PubDate: Sat, 16 Jul 2016 04:08:03 GMT
       
  • Identification of causative agent for fungal infection and effect of
           disinfectants on hatching and survival rate of common carp (C. carpio)
           larvae

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Syeda Maksuda Yeasmin, Md. Anisur Rahman, Md. Mer Mosharraf Hossain, Md. Habibur Rahman and Abdulla-Al-Asif.
      Common carp (Cyprinus carpio) is one of the commercially important and commonly cultured fish. In the hatchery intensive incubation leads to microbial overgrowth in C. carpio eggs that hamper egg development, hatchability and larval survivability. The aim of this study is to find out causes of mass mortality in C. carpio eggs during peak- breeding season between March to May 2015 at Mafatema fish hatchery, Chanchra, Jessore sadar upazilla. In the present study three disinfectants with three different concentrations in each such as methylene blue 1, 3 and 5mg/L., malachite green 1, 3 and 5mg/L., sodium chloride 1, 2 and 3g/L were used to observe the hatching rate of fertilized eggs and survival rate of larvae. Bacterial load of culture water was examined during the induced breeding of C. carpio with mycological examination of egg samples with different disinfectants. The total bacterial count fluctuated from 3.4 x 108 CFU/ml to 32.7 x 108 CFU/ml during the period of fertilization to 4days of hatching. The fertilized eggs infected by Saprolegnia spp. were appeared as tuft hairy like balls with a white cottony envelop. Among all the treatment 1mg/L methylene blue, 3mg/L malachite green and 1g/L sodium chloride showed significantly better (P

      PubDate: Sat, 16 Jul 2016 04:05:59 GMT
       
  • Morphometric characterization of Brahman crossbred cattle and prediction
           of live weight using linear body measurements

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Mahbubur Rashid, Md. Azharul Hoque, Khan Shahidul Huque, Md. Azharul Islam Talukder and A. K. Fazlul Huque Bhuiyan.
      The present work was conducted to evaluate the variability in linear body measurements; to investigate the relationship between body linear measurements and live weight and to predict live weight of F1 Brahman crossbred cattle using body measurements. A total of 123 male and 87 female F1 Brahman crossbred cattle of 6-36 months age and weighing from 63 to 535 kg were used for the study over a period from 2010 to 2014. The study revealed that that most of the morphological measurements were linearly increased with the advances of age. The body weight had highest correlation coefficient with the heart girth around the chest (r=0.96, p12-24 months (r=0.95) group. The stepwise regression models revealed that heart girth singly accounted highest variation (93%) in body weight for all animals. Thus, the general equation for prediction of live weight of Brahman crossbred cattle was Y=4.07HG–356 (±6.96) where Y=live weight (Kg), HG=heart girth around the chest (cm). The regression equations for the live weight were Y=2.71HG–191 (±13.5), Y=4.05HG–357 (±9.77) and Y=4.87HG–471 (±23.0) for ≤12,>12-24 and>24 months age groups. The best model for estimating body weight was obtained using HG and body length (BL) for all animals Y=2.83HG+1.80BL–392 (±6.69). These results suggested that prediction equations based on HG or in combination of HG and BL can be used efficiently in Brahman crossbred cattle to predict live weight.

      PubDate: Sat, 16 Jul 2016 04:01:45 GMT
       
  • Effect of feeding management of broodstock on breeding performance of bata
           (Labeo bata)

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Habibur Rahman, Md. Anisur Rahman, Md. Mer Mosharraf Hossain, Syeda Maksuda Yeasmin and Abdulla-Al-Asif.
      Labeo bata is one of the important minor carps in Bangladesh with great demand as good table fish. The study was conducted to observe the breeding performance at different management practices in Mafatema, Rupali, Modhumoti and Anan fish hatchery and disinfection treatments of water, eggs and fry in Jessore, Bangladesh during 10 March 2014 to 15 May 2015. An improvement in broodstock nutrition and feeding has been shown to greatly improve seed production. Protein and lipid percentage of broodstock diet have been identified as major dietary factors. Protein level was 24.77%, 23.47%, 18.08%, 17.78% and lipid level was 11.07%, 9.50%, 7.74%, 8.14% in Mafatema, Rupali, Modhumoti and Anan fish hatchery respectively. Three concentrations of four chemical-formalin (10, 20, 30 mg/L), malachite green (1, 3, 5 mg/L), NaCl (1, 2, 3 g/L) and methylene blue ( 1, 3, 5 mg/L) treatment regimes and a control were compared for efficacy in treating L. bata eggs to prevent fungus and bacterial infection and improve hatch and survival rate of fry. Highest correlation value between absolute fecundity and body weight (r=.938, p

      PubDate: Sat, 16 Jul 2016 03:59:31 GMT
       
  • Shrimp disease investigation and culture strategies in Bagerhat district,
           Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Azharul Islam Shabuj, Tonusree Bairagi, Abdulla-Al-Asif, Omar Faruq, Md. Rafiqul Bari and Md. Sharif Neowajh.
      The present study was conducted in three upazilla (Bagerhatsadar, Fakirhat and Rampaul) to investigate the shrimp diseases and culture strategies of Bagerhat district in 2012. Post larvae (PL-13/21) from hatchery and natural were used to stock in the study area. The highest mean stocking density was recorded in the ghers of Rampaul (16796±7729.69 PL/ha), where the maximum survival rate was also found (63.00±11.52 %). The highest dose of lime was recorded as 302.58±50.92 kg/ha at Bagerhat sadar; cowdung was recorded as 605.16±118.46 kg/ha at Fakirhat upazilla and urea was recorded as 29.02±5.84 kg/ha (Bagerhat sadar) respectively. The maximum doses of MP and TSP were recorded as 34.58±4.51 kg/ha at Bagerhat sadar and Fakirhat and 27.79±3.71 kg/ha at Bagerhat sadar respectively. No supplementary feed were fed to shrimp, in where culture dependent on natural foods. The highest shrimp production was found in Fakirhat upazila (667±307.05 kg/ha) and the lowest production was in Rampaul upazilla (497±268.97 kg/ha). Sudden change of pH , low dissolved o2 levels, salinity variations, nutritional deficiency and other environmental changes causes yellow head viral disease (YHD), white spot syndrome viral (WSSV), vibriosis, fusarium and protozoan were recorded during the experimental period.

      PubDate: Sat, 16 Jul 2016 03:55:43 GMT
       
  • Comparative study on meristem culture of three potato cultivars diamant,
           cardinal and granula and their shoot formation

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Marufa Khatun, Md. Shahadat Hossain, Md. Khalekuzzan and Amina Rownaq.
      The main aim of this study was to analyze the comparative study on meristem culture of three important potato cultivars, diamant, cardinal and granula and their shoot formation using meristem tips. Apical meristems were isolated from shoot tips of 25-35 days old field grown plants. After surface sterilization the meristems (0.3 mm) were isolated from the shoot tips. After isolation the apical meristems were placed quickly on “M” shaped filter paper bridge in culture tubes containing liquid MS medium supplemented with various concentrations and combinations of different plant growth regulators. Meristems showed their first growth response by increase in size and became greenish white in colour. They continued their growth and developed shoots with roots. In the present investigation, among the three cultivars of potato, cardinal were the best responsive cultivars for the resuming new growth of cultured meristems on MS medium supplemented with KIN (0.4 mg l-1) + GA3 (0.5 mg l-1). Cultivar diamant showed comparatively better growth response in the primary culture and granula showed better results in shoot length formation in MS0 medium. Cultivar cardinal proved to be best potato cultivar in case of meristem culture than diamant and granula cultivars.

      PubDate: Sat, 16 Jul 2016 03:53:01 GMT
       
  • Determination of in-vivo growth kinetics of virulent Newcastle disease
           virus in layer chicken

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Mostofa Kamal, Mohammad Aynul Haque, Shuvho Chakra Borty, AKM Khashruzzaman, Mohammad Nizam Uddin Chowdhury and Md. Alimul Islam.
      The research work was conducted on 105 layer chicks with a view to determine the rate of distribution of neurotropic virulent Newcastle disease virus (NVNDV) in various organs following infection through natural (intranasal, intraocular and oral) and parenteral (intravenous, intramuscular and subcutaneous) routes of inoculation at different ages (7, 15 and 28 days of old). Each bird received a dose of 0.2 ml contained 300 ELD50 of reference NVNDV. The highest body temperature (≥1080F) was recorded in the birds of almost all the experimental groups within 48 to 72 hours of PI. Appearance of clinical signs was observed earlier (48 to 72 hours of PI) in parenterally infected birds than those of inoculated through natural routes. The shortest duration (24-48 hours of PI) and longest duration (74-138 hours of PI) of death time were recorded in birds those inoculated through IV and oral routes of infection respectively. Isolation of NDV was positive from day 2 of PI and onward in all the groups with some minor variations in some cases. The CEF system was found more sensitive for the isolation of viruses compare to that of avian embryo. The highest HA titre of NDV was found in the brain tissue followed by lungs and kidney. Significantly (p

      PubDate: Sat, 16 Jul 2016 03:49:29 GMT
       
  • Prevalence and risk factors of Human Immunodeficiency virus, Hepatitis B
           virus, Hepatitis C virus and Syphilis infections among nonprofessional
           blood donors in Chittagong, Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Zobaidul Alam, Md. Amayan Hossain and Mahmud A. Chowdhury.
      Human Immunodeficiency virus (HIV), Hepatitis B virus (HBV), Hepatitis C virus (HCV) and Syphilis infection are the major public health problem, alarming the most developing countries like Bangladesh. These studies determined the prevalence rate and risk factors of HIV, Hepatitis B, Hepatitis C and Syphilis among nonprofessional blood donors in multiple blood transfusion center of Chittagong. Information was collected by questionnaire to find out the relationship of HIV, HBV, HCV and Syphilis infection with age, sex, socioeconomic status, residence and religious believes. The sero-prevalence of HIV, HBsAg (HBV), anti-HCV (HCV), VDRL (Syphilis) among the non-professional blood donors were 00.00%, 4.88%, 0.92%, and 0.70% respectively. So, the highest prevalence rate was HBsAg and the lowest was HIV. We didn’t find more than one positive case in the same blood sample. The prevalence of HBsAg among the study population, students (120/6) were 5.00%, service holders (73/3) were 4.11%, businessmen (40/4) were 10%, housewives (14/0) were 00.00%, others (farmers, immigrant workers) (40/1) were 2.50%. The highest prevalence of HBsAg was due to illiteracy and unsafe sexual behaviors.

      PubDate: Sat, 16 Jul 2016 03:45:26 GMT
       
  • Effect of nitrogenous fertilizer on yield of mungbean [Vignaradiata (L.)
           Wilczek] in Patuakhali district of Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Mosarref Hossen, A.S.M. Iqbal Hussain, Abdullah –Al –Zabir, Md. Jamil Hossain Biswas and Md. Rakibul Islam.
      An experiment was conducted at the research field of the Horticulture Research Center at Labukhali, Patuakhali during the period from January to March 2014 to find out the most suitable BARI mungbean variety and optimum rates of N concerning higher seed yield under the regional condition of Patuakhali (AEZ–13). Two BARI mungbean varieties namely BARI mung–5 (V1) and BARI mung–6 (V2) and five levels of N fertilizer including control viz. 0 kg N ha–1 (N0), 30 kg N ha–1 (N30), 45 kg N ha–1 (N45), 60 kg N ha–1 (N60), and 75 kg N ha–1 (N75) were used for the present study as level factor A and B, respectively. In case of variety, BARI mung–6 produced significantly longest pod (7.56 cm), maximum pods (9.14) plant–1, maximum seeds (9.14) pod–1, higher weight of 100–seed (4.48 g), highest seed weight (4.33 g plant–1) and highest seed yield (1.56 t ha–1) than BARI mung–5 at harvest. In case of N fertilizer, longest pod (7.96 cm), maximum pods plant–1 (10.45), maximum seeds pod–1 (9.70), higher weight of 100–seed (4.52 g), higher weight of seed (5.73 g plant–1) and greater seed yield (1.85 t ha–1) were also obtained in 45 kg N ha–1 compare other N levels. The BARI mung–6 × 45 kg N ha–1 for seed yield was found under the regional condition of Patuakhali (AEZ-13).

      PubDate: Sat, 16 Jul 2016 03:42:24 GMT
       
  • Effect of planting time on the growth and yield of chia (Salvia hispanica
           L.)

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Masudul Karim, Md. Ashrafuzzaman and Md. Alamgir Hossain.
      Chia (Salvia hispanica L.) contains the highest level of omega- 3 fatty acid available in any cultivated plant source. The objective of this research was to find out the suitable planting time for chia in Bangladesh and to study the effect of planting time on its growth and yield. An experiment was carried out at the field laboratory of the Department of Crop Botany, Bangladesh Agricultural University (BAU), Mymensingh, during the period from November 2013 to July 2014 to study the growth, morpho-physiological yield contributing characters and yield of chia. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. The collected data were analyzed statistically and the means were adjudged by DMRT using the statistical computer package program, MSTAT_C. The tallest plant (136 cm), highest number of inflorescence plant–1 (13.67), highest amount of total dry matter (305 g) and highest root length (7.767 cm) were recorded from November planting at harvest. However, leaf area plant–1 (207.2 cm2) and LAI (1.891) had also significantly higher from November planting at 90 days after sowing (DAS) compared to other planting times. November planting further recorded the higher CGR (33.43 g m-2 day-1) at the stage between 76–90 DAS and thereafter they decreased. Higher seed yield (1033 Kg ha–1), husk yield (888 Kg ha–1), seed yield plant-1 (4.773 g), husk yield plant-1 (2.840 g) were also recorded from November planting. In December planting the seed yield, husk yield, seed yield plant-1, husk yield plant-1 were 681 Kg ha–1, 340 Kg ha–1, 2.350g and 0.9467g, respectively. Lower seed yield (159 Kg ha–1), husk yield (166 Kg ha–1), seed yield plant-1 (0.6150 g) and husk yield plant-1 (0.5383 g) were recorded from January planting. Except total dry matter, other morpho-physiological characters, yield and yield contributing factors were less effective in January than other planting time. February and March planting did not produce any fertile spike resulting yield less vegetative growth. These results revealed that, November planting was found outstanding superiority for plant growth, yield and morpho-physiological characters of chia in Bangladesh.

      PubDate: Sat, 16 Jul 2016 03:38:39 GMT
       
  • Effects of selected vitamins and minerals on growth rate and hematological
           parameters in broilers

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mohammad Harun-Ar-Rashid, Nazim Ahmad, Mohammad Rohul Amin and Mohammad Lalmoddin Mollah.
      The present study was designed with a view to study the body weight and hematological parameters; total erythrocyte count, haemoglobin concentration, packed cell volume and erythrocyte sedimentation rate of growing broilers fed with standard poultry ration. Broilers were kept under close observation for a period of 14 days and body weight was measured at each 7 days interval up to the end of the 35 days of experimental period. A total of 20 (7 days old) broiler chicks were reared throughout the entire period of study. These chicks were randomly divided into four equal groups (n=5). Group A considered as control, fed only standard ration. Group B, C and D was considered as treated group. Group B with vitamin AD3E 1 ml/5 liter drinking water. Group C treated with mineral 1 ml/5 liter dirking water and Group D treated with vitamin (AD3E) 1 ml/5liter and mineral 1 ml/5 liter dirking water with standard ration. It was observed that body weight of broiler increased significantly (p

      PubDate: Sat, 16 Jul 2016 03:10:27 GMT
       
  • Mitigation of the adverse effects of soil salinity in rice using exogenous
           proline and organic manure

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Sukanta Dhar, Mohammad Golam Kibria, M. Mazibur Rahman and Md. Anamul Hoque.
      Salinity causes cellular damage and limits crop productivity. Accumulation of organic compound is one of the major adaptive mechanisms for salinity tolerance in plants. The main objective of the present study was to mitigate the adverse effects of soil salinity in rice through organic amendments like proline and organic manure. The field experiments were conducted at the farmer’s field of Batiaghata upazilla under Khulna district with aman rice. The soil was silty clay loam having pH 7.2, EC 6.6 dS/m, CEC 26 meq/100 g soil and organic matter 0.84%. Rice variety BR 23 was used as a test crop. There were 15 treatment combinations with different doses of proline and organic manures. Recommended doses of N, P, K, S and Zn fertilizers were applied to all the experimental plots. Proline was applied as foliar spray at seedling and/or vegetative stages. Organic manures were added to soils during final land preparation. The experiments were laid out in a randomized complete block design with three replications. Significant reductions in growth and yield of rice were observed under saline conditions. Application of both proline and organic manures significantly increased growth, yield contributing characters, and grain and straw yields of rice under salinity conditions. There were no significant variations in growth and yield of rice due to use of different doses of proline and organic manure. Increased nutrient uptake and K+/Na+ ratio were observed in rice due to proline as well as organic manure application under saline conditions. The present study suggests that exogenous proline or organic manure confers tolerance to salinity in rice by increasing K+/Na+ ratio and nutrient uptake.

      PubDate: Sat, 16 Jul 2016 03:06:19 GMT
       
  • Effect of spent mushroom compost on yield and fruit quality of tomato

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Reema Ashrafi, Md. Rashedur Rahman Rajib, Rajia Sultana, M Mazibur Rahman, Musharrof Hossain Mian and Faria Hossain Shanta.
      An experiment was conducted to observe the performance of the composted spent mushroom substrate (SMS) along with chemical fertilizers on the yield, fruit quality and nutrient uptake by tomato plant for using the mushroom waste through composting. The experiment was laid out in a randomized complete block design with seven replications. Treatments were T1: no fertilizer and compost application (control), T2: recommended dose of fertilizers (RFD), T3: 25% SMC-N+ 75% fertilizer-N, T4: RFD + 2.5 t ha-1 SMC, T5: 50% SMC-N + 50% fertilizer-N, T6: 100% SMC-N and T7: 100% SMC-N + 50% fertilizer-N. Application of SMS compost at 2.5 t ha-1 along with recommended fertilizer dose showed the best performance for number of fruits, fruit yield, fruit quality (total protein, vitamin C, total sugar, reducing sugar) and nutrient uptake by tomato. This treatment showed significantly higher fruit yield, quality and nutrient uptake not only over control but also RFD, SMS compost alone and combination of SMS compost & RFD. Though SMS compost alone proved less effective, however combined application of SMS compost at 2.5 tha-1 with chemical fertilizer of recommended dose had shown to be more effective.

      PubDate: Sat, 16 Jul 2016 03:03:51 GMT
       
  • Microbial and sensory evaluation of Bangladeshi frozen chicken sausage

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Shuvho Chakra Borty, Md. Hiron Khan, Tanvir Ahammed, Md. Ataul Gani, Mohammad Nizam Uddin Chowdhury, Md. Shirajum Monir and Md. Alimul Islam.
      The purpose of this study was to evaluate the microbiological safety and the effect of some synthetic chemicals with comparison to natural additives such as rosemary extract in preventing microbial growth and as a source of antioxidant by maintaining GHP as well as GMP of Bangladeshi processed chicken sausages with low fat content to correlate some scientific evidence in producing cancer and cardiovascular diseases by the consumption of meat product. Two experimental batches of chicken sausage were prepared according to Bangladeshi standard using different parameters of ingredients. Then centesimal parameter, PH and instrumental color of the product were analyzed according to standard protocol. A sensory parameter was also checked by ranking the acceptance level of consumers about different batches of chicken sausage. The sensory properties and the instrumental color parameter of two experimental batches of chicken sausage ‘A’ and ‘B’ were found almost similar in comparison to the commercial cured chicken sausage ‘C’ but quite dissimilar in comparison to commercial uncured sausage ‘D’. Total coliforms were observed 4.4 × 10 for sausage ‘A’ and 5.3 × 10 for sausage ‘B’, while the most probable number (MPN) for E. coli was 1.7 for both products. The counting of sulphite reducing Clostridium was 2 cfu/g and 3 cfu/g for both of the batches ‘A’ and ‘B’ consecutively. The coagulase-positive Staphylococci and lactose fermenting Salmonella was not found in any batches of chicken sausage.The results of this experiment clearly mentioned the possibility of producing safe and high quality chicken sausage with reduced fat content in Bangladesh by using natural pigments and antioxidants.

      PubDate: Sat, 16 Jul 2016 03:01:19 GMT
       
  • Identification and antibiogram study of bacterial species isolated from
           milk samples of different locations in Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Md. Nuruzzaman Munsi, Nathu Ram Sarker, Razia Khatun and Mohammed Khorshed Alam.
      Cow’s milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Gram’s staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.

      PubDate: Sat, 16 Jul 2016 02:56:29 GMT
       
  • Prevalence of common parasitic and infectious diseases of goat at Babugonj
           upazilla, Barisal, Bangladesh

    • Abstract: 2016-07-19T23-35-06Z
      Source: Asian Journal of Medical and Biological Research
      Mohammad Rohul Amin.
      This study was conducted to investigate the prevalence of common parasitic and infectious diseases of goat at Babugonj upazilla, Barisal, Bangladesh. The study was performed in Veterinary Clinic, ANSVM, PSTU and Upazilla Veterinary Hospital, Babugonj, Barisal during June, 2014 to May, 2015. The diseases were diagnosed on basis of clinical signs, owner’s statement, general clinical examinations and laboratory diagnosis. A socio-economic survey of 200 destitute women and marginal/poor farmers of Barisal district was also done using a semi-structured questionnaire on their household. 70% of the respondents were females while 30% were males. 65% respondents were involved in agriculture, 13% in own business, 3% in shared business, 2% in govt. service and 17% in non-govt. service. 39% respondents had knowledge about signs of health of goat followed by management (74%), goat diseases (24%), veterinary drugs (20%), animal vaccine (14%) and biosecurity (13%). 53% animal houses were kacha followed by tin (41%) and pukka (2%). Goat received feed from own fodder areas (52%), purchase concentrate (5%) and both (43%). Respondents used tube-well (24%), river (18%), canal (26%) and ponds (32%) as the source of water for their goat. The prevalence of parasitic diseases was 41.33% followed by infectious diseases (39.34%) in goat. The prevalence of parasitic diseases was highest in rainy season 51.28% followed by autumn (41.61%), summer (40.37%) and winter (27.98%). The prevalence of infectious diseases was highest in winter 52.60% followed by autumn (37.55%), summer (37.47%) and rainy season (32.42%). Among viral diseases, prevalence of PPR was highest 8.52% followed by FMD (6.81%), goat pox (2.68%), contagious ecthyma (1.76%) and rabies (0.25%). The prevalence of peste des petits ruminants (PPR) was highest in winter (15.86%). The prevalence of pneumonia was highest 8.71% in whole year among the bacterial diseases. The prevalence of fungal disease (ringworm) was 1.44% in goat. The prevalence of trematodiasis was 12.60% followed by nematodiasis (11.27%), babesiosis (2.18%), tape worm infection (2.03%) and coccidiosis (1.19%). The prevalence of ecto-parasitic diseases was 12.06% .The prevalence of trematodiasis was highest in rainy season (18.01%). The prevalence of babesiosis was highest in summer (3.81%) and coccidiosis in winter (3.81%). The prevalence of ecto-parasitic diseases was lowest in winter (8.88%). The results of the present study will be helpful for scientists, extension service providers and veterinary practitioners for designing appropriate control measures for such diseases of goat.

      PubDate: Sat, 16 Jul 2016 02:53:21 GMT
       
 
 
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