for Journals by Title or ISSN
for Articles by Keywords
help
Followed Journals
Journal you Follow: 0
 
Sign Up to follow journals, search in your chosen journals and, optionally, receive Email Alerts when new issues of your Followed Journals are published.
Already have an account? Sign In to see the journals you follow.
Journal Cover Advance Laboratory Medicine International
  [1 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2228-8961 - ISSN (Online) 2228-8961
   Published by ScopeMed Homepage  [97 journals]
  • Combined osmotic fragility (OF) and dichlorophenol-indolephenol (DCIP)
           tests for screening for hemoglobin disorder: a reappraisal on the
           diagnostic property from real usage.

    • Abstract: 2016-01-18T23-16-48Z
      Source: Advance Laboratory Medicine International
      Beuy Joob.
      Hemoglobin disorders is an important public health problem in Thailand. There have been several trials on many screening methods for detection of hemoglobin disorder among Thai pregnants and good screening property of combined method between osmotic fragility (OF) and dichlorophenol-indolphenol (DCIP) can be seen. For a few years, the combined test has been launched to the rural hospital communities of Thailand, where the high prevalence of disorder is documented. Here, the author performed a reappraisal on usefulness of the program in Thai rural hospitals. According to this study, 3 complete reports on the using of OF and DCIP tests screening for antenatal clinic in rural communities were recruited for further study. All 4,902 pregnant subjects were screened and 2,232 got positive screening results (45.5 %). Of overall 2,232 positive cases from screening, 1,243 cases with confirmed disorder could be detected. According to this study, the overall positive predictive value for the real usage of the combined screening test is about 55 %. However, the sensitivity and specificity of the test cannot be calculated due to the lack of reported data. The expected possibility of any pregnant subjects visiting the antenatal clinic in this study to have hemoglobin disorder is equal to 25.02 %.

      PubDate: Fri, 15 Jan 2016 06:07:51 GMT
       
  • Labile plasma iron and transferrin saturation as iron overload markers

    • Abstract: 2015-12-14T23-17-32Z
      Source: Advance Laboratory Medicine International
      Debwasah Shutty, Roger Durman.
      This letter to editor discuss on usefulness and limitations of labile plasma iron and transferrin saturation as iron overload markers

      PubDate: Thu, 10 Dec 2015 20:05:35 GMT
       
  • Serum vascular endothelial growth factor to platelet count ratio after
           garlic supplementation in patient with lung cancer

    • Abstract: 2015-10-31T00-22-22Z
      Source: Advance Laboratory Medicine International
      Mohandras Benopula, Kazoles Hendra, Adica Elene.
      This short letter to editor report experience of author on garlic supplementation in a patient with lung cancer. The observation on serum vascular endothelial growth factor to platelet count (VEGF/PC) ratio in garlic supplementation in this patient is reported.

      PubDate: Mon, 26 Oct 2015 22:12:19 GMT
       
  • Oral glucose tolerance test: a case study on pre-analytical factor
           interference due to lemonade adding

    • Abstract: 2015-10-16T23-21-12Z
      Source: Advance Laboratory Medicine International
      Maximo Beraza, Horares Larestha.
      Oral glucose tolerance test (OGTT) is a useful test in diagnosis of diabetes mellitus. Here, the author presented an interesting case study on pre-analytical factor interference on OGTT. Some additional information on the preanalytical error for OGTT is also quoted in this article.

      PubDate: Tue, 13 Oct 2015 06:10:13 GMT
       
  • Evaluation of the flow cytometric osmotic fragility in the diagnostic of
           hereditary spherocytosis

    • Abstract: 2015-09-22T23-16-39Z
      Source: Advance Laboratory Medicine International
      Mohammed Nazim Bennaoum, Hassiba Lazreg, Affaf Adda.
      BHereditary spherocytosis is a constitutional hemolytic anemia due to the presence of spherical red cells in the blood secondary to a genetic defect of some membrane proteins. The test commonly used in the screening is the determination of the osmotic fragility. Practice this test is difficult to interpret and lack of sensitivity and specificity. The purpose of this study is to evaluate a new and simple method using flow cytometry for the determination of osmotic fragility. The principle of the test is to determine the percentage of residual red cells after the addition of deionised water which acts as an hemolysis-inducing agent. We therefore studied a group of 94 patients with 5 known cases with hereditary spherocytosis. Each sample was tested by osmotic globular resistance dilution analysis and by flow cytometry. The results are compared by PEARSON's correlation.The test has a sensitivity of 100% and a specificity of 96%. The correlation coefficient is -0.626 for the entire population (P

      PubDate: Tue, 22 Sep 2015 21:27:08 GMT
       
  • Groundnut oil as an alternative clearing agent to xylene in histological
           tissue processing

    • Abstract: 2015-08-14T04-21-26Z
      Source: Advance Laboratory Medicine International
      Ebenezer OLubunmi Esan, Ayodeji Blessing Ajileye, Samuel Ayobami Fasogbon.
      This research work was conducted to compare the tissue processing suitability of groundnut oil as a clearing agent with that of tissue processed with xylene as a clearing agent. Tissue samples were obtained from the liver, lungs, and small intestine of adult wistar rats. The tissue samples were processed with groundnut oil as a clearing agent and the other tissue samples which were also processed along with xylene as a clearing agent for a period of 4 hours to 24 hours using a manual tissue processing method. All the tissue samples processed with both groundnut oil and with xylene as a clearing agents were all sectioned with a rotary microtome and stained with Masson’s Trichrome and Haematoxylin and Eosin methods. Tissue samples labeled G1, G2 and G3, were cleared with Groundnut oil during tissue processing and these tissue samples were processed, but not as well as those tissue samples labeled X1, X2, X3, cleared with xylene during tissue processing. The tissue samples cleared and processed with Groundnut oil did not alter the histochemical staining of tissues. Simple staining techniques like Masson’s Trichrome and haematoxylin and Eosin were employed. The results showed that there was no difference between tissue sections cleared in Groundnut oil and those tissue sections cleared in Xylene. Groundnut oil, which is a cheap, easily available and non-toxic agent is suitable and can be used as a clearing agent in histological tissue processing.

      PubDate: Thu, 13 Aug 2015 04:33:58 GMT
       
  • Repeat endoscopy in Barrett's esophagus with no dysplasia and low-grade
           dysplasia

    • Abstract: 2015-06-24T23-32-38Z
      Source: Advance Laboratory Medicine International
      Abdulla Mohammad Shendal.
      This letter to editor briefly discusses on the clinical advantage on performing Repeat endoscopy in Barrett's esophagus with no dysplasia and low-grade dysplasia.

      PubDate: Sun, 21 Jun 2015 21:05:26 GMT
       
  • Diagnostic error in health care: an issue that requires continuous concern

    • Abstract: 2015-05-27T14-20-54Z
      Source: Advance Laboratory Medicine International
      Theenar Hares Shokar.
      This letter to editor briefly discusses on the common problem, diagnostic errors in healthcare.

      PubDate: Mon, 25 May 2015 21:36:37 GMT
       
  • Correlation between fever and leukocyte in dengue infection, a clinical
           observation

    • Abstract: 2015-05-13T04-17-31Z
      Source: Advance Laboratory Medicine International
      Sophiank Vong, Arun Kumar Srininath, Rutheran Koff Rodoff.
      Dengue infection is a major public health problem, affecting general population in the Southeast Asia Region. The haematologic aberration including lymphocytosis, coagulopathy and predominant thrombocytopenia are the common manifestation in the patients with dengue infection. Here, the author reported the experience about fever and leukocyte count of 30 patients with dengue infection who visited the author during endemic season, 2013. The average level of leukocyte among these patients was studied. The correlation between the recorded body temperature of the patients and the leukocyte level was investigated. We found that the fever is common in the patient with dengue infection and the lymphocytosis, not leukocytosis is the common leukocyte disorder in these patients. The correlation between body temperature and total white blood cell count and differential white blood cell count is poor and not significant. Therefore, the fever and the leukocyte disorder in the patients with dengue fever is only a co - presentation.

      PubDate: Wed, 06 May 2015 00:40:58 GMT
       
  • Role of immunohistochemistry in differentiating muscularis mucosae and
           muscularis propria as an aid to accurate staging of invasive urothelial
           carcinoma: a pilot study

    • Abstract: 2015-02-23T09-31-21Z
      Source: Advance Laboratory Medicine International
      Prateek Kinra, Ajay Malik, Vibha Dutta.
      The common prognostic markers for urothelial neoplasms include- tumour grade, invasion into muscle, vascular invasion, histological type, lymph node status and currently molecular markers. However the single most important prognostic marker that has stood the test of time is the extent of invasion into muscularis propria. Treatment of bladder carcinoma is determined by the stage (depth of invasion). Distinguishing bladder muscularis propria from muscularis mucosae can be problematic especially in small transurethral resection specimens cut tangentially. Florid desmoplasia and hyperplastic muscularis mucosa is commonly mistaken for muscularis propria. The sections of 70 cases of urothelial carcinoma were subjected to immunohistochemistry by the three immunohistochemical stains [vimentin (for mesenchymal cells-myofibroblasts), Smooth Muscle Actin (for smooth muscle cells) and smoothelin (specific for muscularis propria)]. Our study showed a sensitivity of 98.23% & specificity of 98.13% for the combination of the three immunohistochemical markers used together in accurately assessing the invasion into muscularis propria. Smoothelin positive predictive value for muscularis propria was very high (96.49%) whereas very low (2.8%) for muscularis mucosa. Immunohistochemistry picked up 3/70 cases that were wrongly diagnosed as invasive (florid desmoplastic response was mistaken for muscularis propria layer). Vimentin showed a moderately high sensitivity and specificity (accuracy of 81.25%) for myofibroblasts in the neighbouring area around tumour thereby assisting in picking up desmoplasia. Immunohistochemistry panel composed of SMA, smoothelin, and vimentin should be used as per the algorithm suggested. This will aid in accurately labelling the muscularis propria and rule out false positive & false negative invasions.

      PubDate: Wed, 18 Feb 2015 03:53:44 GMT
       
  • Facial demodex infestation among HIV seropositive and seronegative
           subjects

    • Abstract: 2015-01-29T09-34-48Z
      Source: Advance Laboratory Medicine International
      Beuy Joob, Chandrigra Singaraja.
      The skin disorder is usually an important problem in the patients with the problem of impaired immunity. The superficial dermatological problems can be seen in HIV infected patients but is not commonly mentioned in dermatology. In fact, in HIV infected cases, several problems can be seen such as onychomycosis, Tinea capitis, etc. Here, the authors report on the observation of facial demodex infestation, a rarely mentioned ectoparasitosis, among HIV seropositive and seronegative subjects. A small preliminary survey with improved transparent tape method was performed on 50 HIV seropositive and seronegative subjects. The prevalence rate of facial demodex infestation among HIV seropositive and seronegative subjects are equal 74 % and 46 %, respectively. Based on the present study, the similar prevalence of infestation among seronegative subjects to the previous publication on prevalence among healthy adults (44 %). Focusing on the prevalence among HIV infected patients, it is significantly higher than the rate among HIV seronegative group. This report is the pioneer work that addresses the importance of high prevalence of demodex infestation among HIV seropositive patients.

      PubDate: Thu, 29 Jan 2015 04:28:55 GMT
       
  • Histopathological evaluation of renal biopsy in a tertiary care center in
           South India

    • Abstract: 2014-11-13T08-33-21Z
      Source: Advance Laboratory Medicine International
      Nisha Jayashankar Marla, Ramesh Naik, Prashanth Kumar, Hilda Fernandes, Jayaprakash CS, Kirana Pailoor.
      Current study was done to evaluate the histopathological findings in glomerulopathies and to correlate the histopathological findings with the clinical disease. Thirty cases of renal biopsy were studied. All biopsies were collected in Bouin’s fluid and processed routinely. Slides were stained routinely with hematoxylin and eosin (H and E). Periodic acid Schiff (PAS) and Methanamine silver (PASM) were done as and when required. Immunofluorescence was performed and was correlated with clinical and microscopic findings in all cases. Among thirty renal biopsies, primary glomerulonephritis accounted for 70% and secondary glomerulonephritis accounted for 30%. Among primary glomerulonephritis, most predominant lesion was focal segmental glomerulosclerosis(FSGS) (28.5%) and post infectious glomerulonephritis (28.5%) followed by minimal change disease(MCD) (14.3%), membranous glomerulonephritis (9.5%), crescentric glomerulonephritis (4.8%). membranoproliferative glomerulonephritis (4.8%), immunoglobulin A nephropathy (4.8%), chronic glomerulonephritis (4.8%). Among secondary glomerulonephritis predominant lesion was systemic lupus nephritis (66.7%) followed by diabetes mellitus (22.2%) and amyloidosis (11.1%). Among primary glomerulonephritis increase in occurrence of focal segmental glomerulosclerosis and post infectious glomerulonephritis was noted. Among secondary glomerulonephritis increase in occurrence of systemic lupus erythematosus was noted.

      PubDate: Tue, 11 Nov 2014 16:20:29 GMT
       
  • Clinico-pathological study of adnexal tumors of skin

    • Abstract: 2014-11-13T08-33-21Z
      Source: Advance Laboratory Medicine International
      Nisha Jayashankar Marla, Ramesh Naik, Prashanth Kumar, Hilda Fernandes, Jayaprakash CS, Kirana Pailoor.
      The aims of this work is to determine the spectrum of histopathological patterns of Adnexal tumors of skin, to characterise the tumors histologically according to World Health Organization (WHO) classification system for skin adnexal tumors and to analyse the tumor with regard to behaviour, anatomic location and sex of the patient. The study was conducted retrospectively at the department of Pathology; in a Tertiary care Hospital in South India. Study duration was 3 years from June 2009 to June 2012. All the Adnexal tumors received during this period were collected and Hematoxylin and Eosin and slides of special stains of the same were reviewed and lesion were characterised according to WHO classification system for Skin Adnexal Tumors. 40 cases of Adnexal tumors were diagnosed during the study period. 36 cases (90%) were benign, while 4 cases (10%) were malignant. Tumors were observed more commonly in females with M: F ratio of 1.5:2. Most tumors were localized to head and neck region (62.5%), followed by trunk and upper limb (27.5%), abdomen and lower limb (7.5%) and labia majora (2.5%). Most common tumors were Eccrine tumors 18 cases (45%), followed by 12cases (30%) of Pilar tumors, 7 cases (17.5%) of Apocrine tumors and least common being tumors with sebaceous differentiation 3 cases (7.5%). Practically adnexal tumors of Skin have been historically classified into 4 groups. However, in some cases, the diagnosis of Adnexal neoplasm presents unique difficulties. Although most of these tumors are benign, it is important to diagnose them accurately, if incompletely excised can recur and evaluation of margins is recommended.

      PubDate: Tue, 11 Nov 2014 16:16:23 GMT
       
  • Dynamics of combined oral contraceptive: a study of some biochemical
           parameters in female wistar rats

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Joseph Emberga Toryila, Amadi K, Odeh SA, Adelaiye AB, Egesie UG, Achie LN.
      Contraception is an important health issue in preventive medicine because it protects women globally from the effects of unwanted pregnancy and allows them to integrate into society. Uncontrolled childbearing in underdeveloped countries carries a significant risk of maternal death. Combined oral contraceptive (COC) is the most reliable method of contraception. COCs are currently among the most commonly used drugs in developed countries; over one hundred million women worldwide are currently using oral contraceptives. Estrogens and oral contraceptives are both associated with several liver related complications including intrahepatic cholestasis, sinusoidal dilatation, hepatic adenomas, hepatocellular carcinoma, hepatic venous thrombosis and an increased risk of gallstones. These side effects are more common with higher doses of estrogens, as were used in the early high-dose estrogen formulation of oral contraceptives, but they have also been described with use of more modern birth control pills and with low dose, estrogen hormonal replacement therapy. This research priority included efforts to discover the effect of COC (DUOFEM) on biochemical parameters. Eighty (80) female wistar rats aged 10-12 weeks weighing 180-250 g were used for the study. They were divided into four groups of 20 rats each comprising 10 treated and 10 control rats. The treated rats received 0.6mg/kg body weight of COC intragastically for 36, 48, 60 and 72 days in five-day cycles (four-days treatment with one-day break). The COC was given intragastically in 5-day cycles (4-day treatment with 1-day break). All controls were given fresh water at ad libitum daily for the period of the experiment. Serum electrolytes determined using Audicom AC99000 and liver function tests by ELISA method. Liver and kidney tissues were stained with haematoxylin and eosin. Serum Alkaline Phosphatase (ALP) Aspartate aminotransferase (AST) Alanine aminotransferase (ALT) were all elevated in treated groups compared to controls (P

      PubDate: Fri, 29 Aug 2014 07:51:39 GMT
       
  • Chromogranin A – containing endocrine cells in the gastric mucosa of
           patietns with Helicobacter pylori associated chronic gastritis type B

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Sevgi Bakaris, Ali Çetinkaya.
      The aim of the present study was to examine the chromogranin A containing endocrine cells in gastric mucosa of patients with Helicobacter pylori associated chronic gastritis type B, histological inflammation and interactions among these parameters. In 35 patients (12 males and 23 females, median age 47 years, range 21-86 years) the gastric mucosal tissue was obtained by endoscopic biopsy from the antrum and corpus. Slides were stained with haematoxylin and eosin, alcian blue (pH 2.5), periodic acid Schiff, modified Giemsa for Helicobacter pylori, and chromogranin A to visualize endocrine cells. Biopsy specimens were assessed histologically according to the Sydney system. The mean number of stained cells was calculated by counting the number of cells per visual field when using a x 40 objective and a x10 eye piece. Cell counts were made on two visual fields in one section from each specimen (three specimen per site). We studied 24 patients positive for Helicobacter pylori and 11 negative patients. The median chromogranin A containing endocrine cell number in antrum and corpus mucosa was significantly lower in Helicobacter pylori positive than in Helicobacter pylori negative patients (respectively p< 0.01, p< 0.05). There was an inverse correlation between endocrine cell numbers and Helicobacter pylori of the gastric mucosa. On the other hand, endocrine cell numbers of the antral gastric mucosa significantly decreased with inflammatory change associated with Helicobacter pylori gastritis but did not in corpus. This decrease was correlated with the grade of active inflammation and chronic inflammation, which was in close relation to Helicobacter pylori infection. Besides endocrine cell were irregularly distributed, close to inflammatory areas, or near lymphoid follicles. These results indicate that, suggesting a close relationship between Helicobacter pylori and gastric chromogranin A regulation. A close correlation between an decrease in gastric chromogranin levels and the histologic activity was also present, suggesting that certain peptide-immune reactions in the gastric mucosa exist in HP infection.

      PubDate: Thu, 21 Aug 2014 02:43:00 GMT
       
  • Genotoxic Effects of Antidepressant Reboxetine in Human Peripheral
           Lymphocytes

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Lale Dönbak, Osman Turan Dal, Mustafa Çelik.
      Antidepressants are drugs of long-term use to treat various psychiatric disorders such as depression, anxiety, insomnia, chronic pain and are administered to numberless patients. There is some evidence that these drugs might cause the occurence of a mutagenic and/or genotoxic effects. In this study, possible genotoxic effect of reboxetine (Edronax)- a noradrenaline reuptake inhibitor (NARI)-was investigated by using the sister chromatid exchanges (SCEs), chromosomal aberrations (CAs), and micronucleus (MN) analysis. The levels of SCE, CA, and MN were determined in human peripheral lymphocytes treated with five different concentrations of reboxetine (25, 50, 75, 100, and 125 µg/ml) for both 24 and 48 h and compared with those of negative control. SCE frequency was significantly higher in cells treated with higher concentrations of reboxetine with regard to control. Elevation in the level of CA showed correlation with the increasing reboxetine concentrations both in 24 and 48 h treated cells. Similarly MN frequeny was significantly increased in a dose dependent manner in cells treated for 48 h. However, replication index (RI) and nuclear division index (NDI) were not affected by treatment of reboxetine. Obtained results indicate that reboxetine may demonstrate genotoxic activity in human cells.

      PubDate: Thu, 21 Aug 2014 02:40:20 GMT
       
  • Clinicopathologic relevance of angiogenesis and mast cells in basal cell
           carcinomas of skin

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Sevgi Bakaris, Muharrem Bitiren.
      Basal cell carcinoma (BCC) is the most common malignant tumor of the skin. We aim to investigate the correlation between tumor vascularization and mast cells with clinicopathologic parameters in BCC. Intratumoral microvessels were stained with anti-CD34 antibody and mast cells with toluidine blue in 45 BCC lesions. A significant correlation was noted between microvessels density (MVD) and mast cell density (MCD) values (p

      PubDate: Wed, 20 Aug 2014 05:20:07 GMT
       
  • Genotoxic risk of workers from offset printing offices

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Lale Dönbak, Hatice Avan, Ahmet Kayraldız.
      In the present study, a cytogenetic monitoring was carried out to investigate genotoxic risk of workers occupationally exposed to chemicals used in offset printing. The levels of chromosomal aberrations (CA), sister chromatid exchanges (SCEs), and micronuclei (MN) were analysed in the lymphocytes from 30 male workers. The results were compared with a control group consisting of 20 subjects similar to workers with respect to gender, age, and habit. The mean frequencies of CA, SCE, and MN were significantly higher in workers (P

      PubDate: Wed, 20 Aug 2014 05:17:20 GMT
       
  • Evaluation of reference values and biological variations for neuron
           – specific enolase in sterile human urine

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      ergul belge kurutas, Meral Miraloglu.
      The analytical, intra-individual and inter-individual variations for neuron-specific enolase (NSE) were determined and the reference values were established in sterile human urine. A total 400 apparently healthy people, 192 male and 208 female (from 25 to 63 years, average 44 years old), were randomly selected from villages and cities of the southern part of Turkey. Reference values of NSE were established from this population. Furhermore, biological variations of NSE were assessed in 20 apparently healthy subjects for over thirty-day period. NSE activity in spot urines of all individual were determined by ELISA method. The mean value (standard deviation) of the population investigated for NSE was 0.496±0.136 ng/mg Cr. Furhermore, the analytical, intra-individual and inter-individual variations were assessed in 20 apparently healthy subjects and were found to be; NSE: 5.0 %, 20.2% and 14.2%. The results of the index individuality showed that reference values of NSE could be used for diagnostic purposes in various diseases.

      PubDate: Wed, 20 Aug 2014 05:14:25 GMT
       
  • Workload measurement in governmental primary health care medical
           laboratories- Gaza Strip, Palestine

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Reem Tawfeek Abu Shomar.
      A realistic and accurate assessment of laboratory workload is necessary for effective laboratory management. Workload, the sum of the work achieved or to be achieved, is obtained by multiplying the raw count of each individual procedure by its unit value expressed in units (minutes). For many years, there had been dissatisfaction with the existing method of assessing workload since it doesn't reflect the complexity which varies from test to test. The objective of this work is to develop workload measurement through determination of workload unit values for laboratory tests performed at PHC medical Laboratories and once developed, it will serve as a management tool. Methodology: Time studies were performed by well trained medical technologists on tests performed by Primary Health Care (PHC) laboratories. The study presented the results of unit values per test. An examples were the unit values for Hb, CBC, WBCs, ESR, and Blood grouping & Rh which were 3.5, 2.7, 6.0, 3.3, and 4.1 minutes respectively. Also for Glucose, Urea, Creatinine, Uric Acid, Cholesterol, and Triglyceride the results for unit values were 6.3, 7.9, 8.1, 6.6, 6.5, and 6.5 minutes respectively. The authors recommend utilization of workload unit values determined through the conduction of time studies to develop workload measurement system in the governmental medical PHC laboratories.

      PubDate: Mon, 16 Jun 2014 05:23:34 GMT
       
  • Cytological appraisal of male thyroid lesions in a tertiary care center

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Deepak Kumar B, Vani BR, Padmapriya Kasukurti, Srinivasa Murthy V.
      It is an established fact that although the prevalence of thyroid nodules is lower in males, the risk of malignancy in males with nodules is significantly higher. Fine needle aspiration (FNA) cytology is recommended as the initial diagnostic test for such patients because of its simplicity, reliability, sensitivity and specificity. This study was undertaken to determine the proportion of various thyroid lesions in males based on FNA in our set up; to highlight the importance of FNA as an initial diagnostic tool based on its accuracy, sensitivity and specificity in male sex and to discuss the gender variability of thyroid malignancy. A retrospective analysis of male thyroid lesions was conducted over a 30-month period from Jan 2011 to June 2013. FNA aspiration results were classified into six groups as Non-diagnostic or unsatisfactory, Benign, Atypia of undetermined significance, Follicular Neoplasm or suspicious for a follicular neoplasm, Suspicious for malignancy and Malignant. Surgical pathology result was categorized as benign or malignant with specific subtyping of lesions. 89 cases of male thyroid lesions were studied cytologically, out of which histopathological correlation was available in 17% of cases. 13.7% of malignancies were detected. FNA showed a sensitivity of 75%, specificity of 85%, accuracy of 80%., PPV of 85%, NPV of 75%, FPR of 14% and FNR of 25%. FNA in male thyroid proves to be a very valuable and readily available tool for evaluating thyroid lesions with a high sensitivity, specificity and accuracy, and is of immense help in defining the extent of surgery.

      PubDate: Thu, 15 May 2014 07:17:32 GMT
       
  • Could adenosine deaminase and xanthine oxidase activity be an indicator of
           active hepatitis B'

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Selma Ates Guler, Fatma İnanc Tolun, Melike Yavuz.
      The aim of the present study was to investigate the serum levels of ADA and XO in the patients with chronic active hepatitis (CAH) and inactive hepatitis (IH) and to compare the findings with the healthy controls. Association between the activity of these enzymes and the severity of disease was also evaluated. This study consisted of 95 chronic hepatitis B patients and 67 healthy controls. Patients were divided into two groups as IH (n=45) and CAH (n=50). ADA and XO activities were measured using spectrophotometry. While the levels of ADA and XO were significantly different between the controls and the CAH and also with IH patients (p=0.002, p=0.000), no significant differences were noted between the CAH and IH groups. There were also weak correlations between serum ADA and DNA, and also between serum ADA and XSO levels (r=-0.283, p= 0.046; r=-277, p= 0.05). increased levels of ADA and XO can be an indicator of hepatocyte damage in the patients with hepatitis. However, since there was no significant difference between CAH and IH groups based on the levels of these enzymes, they cannot be considered as useful tools in the differentiation of active and inactive hepatitis.

      PubDate: Sun, 20 Apr 2014 05:41:32 GMT
       
  • Evaluation of Albumin/Globulin and De Ritis ratios as the cost effective
           tools for differential diagnosis of hepatitis

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Gadasala Soma Sekhar, Srinivasan AR, Mohamed Hanifah, Niranjan Gopal.
      The study was primarily undertaken to check the efficacy of De Ritis ratio as a simple, sensitive, reliable and economical biochemical indicator of liver diseases especially alcoholic liver disease. The study was also focused on the controversy surrounding De Ritis ratio as an unequivocal marker of liver diseases. Our study indicated that the De Ritis ratio based on the levels of non-functional proteins is a better indicator of liver diseases in comparison to the ratio of Albumin and Globulin (based on plasma functional proteins). De Ritis ratio values were 1.01+ 0.24, 1.49+ 0.74 and 2.39+ 0.94** respectively with viral hepatitis, non viral hepatitis and Alcoholic liver disease. De Ritis ratio was highly significant as also the Total bilirubin (p-value 0.000 for both). Hence, the De Ritis ratio could be used in association with serum total bilirubin in differentiating Alcoholic liver disease (ALD) from viral (VH) and non viral hepatitis (NVH). The levels of Total protein and Albumin in serum and also the Albumin/ Globulin ratio were insignificant, thereby implying that the ratio of the Non-functional proteins (AST/ALT—the de Ritis ratio) could be used as an independent and reliable indicator of liver diseases in general and ALD in particular.

      PubDate: Thu, 10 Apr 2014 07:28:06 GMT
       
  • Relationship between antioxidant capacity and oxidative stress in patients
           with chronic hepatitis B

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Selma ATES GULER, Fatma Inanc Tolun, Hasan Ucmak, Gulcan Haskaya, Melike Yavuz.
      The hepatitis B virus is a significant pathogen that causes cirrhosis, and hepatocellular cancer as a result of the damage it causes to liver cells. Its infection affects more than 400 million people globally. Although there is an effective vaccine and treatment methods, almost 1, 000, 000 people die every year. We aimed to evaluate the oxidant and antioxidant systems in patients with various clinical forms of chronic hepatitis B infection (CHB). 50 patients with inactive hepatitis B surface antigen (HbsAg) carrier state (IHBCS), 44 patients with CHB and 50 healthy adults were included in this study. The following values were measured and compared in all groups: Glutatyon peroksidaz (Gpx), myeloperoxidase (MPO), catalase (CAT), nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA). In patients with chronic hepatitis B, these values were compared with groups. Alanine aminotransferase (ALT), MPO, MDA levels were higher in the CHB group compared to the other groups (p values

      PubDate: Fri, 07 Mar 2014 02:05:43 GMT
       
  • Biological variations of Xanthine oxidase and myeloperoxidase in sterile
           human urine

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Meral Miraloglu, Ergul Belge Kurutas, Pınar Ciragil.
      The analytical, intra-individual and inter-individual variations were determined for xanthine oxidase (XO) and myeloperoxidase (MPO) and the reference values were established. A total of 380 apparently healthy people, 177 male and 203 female (from 20 to 82 years, average 40 years old), were randomly selected from villages and cities of the southern part of Turkey. XO and MPO activities in human sterile urine samples were measured by spectrophometric method. The distributions were Gaussian and no significant difference was observed between the male and female subjects. The mean value (standard deviation) of the population investigated for XO was 414.0 (93.3) U/L, and for MPO 104.5 (49.2) U/L, respectively. The analytical, intra-individual and interindividual variations were assessed in 20 apparently healthy subjects and were found to be; XO: 6.2 %, 21.2%, and 19.2% MPO: 4.0%, 16.4%, 11.7% respectively. The results of the index individuality showed that reference values of XO and MPO could be used for diagnostic purposes in various diseases.

      PubDate: Fri, 24 Jan 2014 17:38:47 GMT
       
  • The laboratory diagnosis of dengue virus infection, a review

    • Abstract: Publication year: 2014
      Source:Advance Laboratory Medicine International
      Kameel Mungrue..
      Since no protective vaccine or specific treatments are available for dengue fever accurate diagnosis is critical for the early initiation of specific preventive health measures to curtail epidemic spread and reduce economic losses as well as the timely monitoring of patients to prevent fatalities. Commonly used diagnostic methods for confirming dengue infection involve virus isolation, detection of virus antigen or RNA, and the presence of dengue virus-specific antibodies.

      PubDate: Thu, 05 Dec 2013 07:04:22 GMT
       
  • Adenosine deaminase and its isoenzyme as a diagnostic marker in tubercular
           meningitis

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Mrinal Pal, Subinay Datta, Chinmoy Ghosh, Partha Sarathi Das, Tarasankar Malik.
      Mortality and residual morbidity is quit high in tubercular meningitis particular in children. Several investigations have been developed for early diagnosis but this sill poses a lot of diagnostic problem in developing countries like India. So, the present study was performed to search a quick and definite investigation for easy and rapid diagnosis of tubercular meningitis and CSF ADA and its isoenzymes activity was selected to differentiate between tubercular and non-tubercular meningitis. Among 57 meningitis patients, 27 were tubercular, 13 were pyogenic, 12 were viral and 6 were cerebral malaria selected. Cerebrospinal fluid total ADA and ADA2 activity were measured and compared with 23 healthy controls and within the infective meningitis. The CSF ADA activity was significantly elevated in all infective meningitis than control (p

      PubDate: Thu, 05 Dec 2013 07:01:45 GMT
       
  • Pre-preanalytical concerns on the MonoSED - SR for the new MicroSed-SR
           erythrocyte sedimentation rate analyzer

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Sora Yasri.
      The erythrocyte sedimentation rate (ESR) is a time-honored blood test, useful in laboratory medicine. There are several new techniques developed for determination ESR at present. Automated ESR analyzer have been launched for a few years and approved for the laboratory diagnostic property. Here, the authors reported the mechanical consideration and pre-analytical user response to the new Automated ESR analyzer. All of the phlebotomist (8 phlebotomists) noted that this new vacuum tube is not comfortable for usage due to two reasons; a) the length of the tube exceeds the fully extension span between thumb and index fingers and b) the diameter of the tube is too small comparing to the standard needle holder. They said that these two reasons bring the delay in the venipuncture process for 1 – 2 minutes/ cases. The tube length of MonoSed-SR is 2 times about greater than the length of the needle holder and the diameter of MonoSed-SR is about a half of that of the needle holder. These facts make the users felt uncomfortable to use the tube. In addition, mechanical fulcrum can be expected and can bring the failure of venipuncture.

      PubDate: Thu, 31 Oct 2013 02:14:55 GMT
       
  • Biomarkers for neurofibromatosis

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Faik İlik.
      Biomarkers provide a dynamic and powerful approach to understanding the spectrum of neurological disease with applications in observational and analytic epidemiology, screening, diagnosis and prognosis. Biomarkers would be useful to verify NF1 diagnosis, monitor tumor burden, and/or detect transformation. This brief review describes the major uses of biomarkers in clinical investigation.

      PubDate: Sun, 22 Sep 2013 21:14:28 GMT
       
  • Serum vaspin and insulin resistance: predictors of steatosis and fibrosis
           in Egyptian patients with genotype 4 chronic hepatitis C

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Ragaa Abdelkader Ramadan.
      Individual variation in the course of chronic hepatitis C (CHC) is remarkable with implication of host, viral and metabolic factors as well as a suggested genotype dependent pattern. Vaspin and leptin, members of adipokines, are involved in regulation of inflammatory response and insulin resistance (IR) which has a potential role in CHC pathogenesis. Vaspin and leptin levels, viral load, and homeostasis model assessment of insulin resistance (HOMA-IR) were assessed in non-diabetic non-cirrhotic CHC-4 Egyptian patients to recognize variables independently associated with IR, steatosis, and fibrosis. This study included 175 subjects; 30 healthy volunteers with age 43.4±4.2 years, and 145 CHC-4 patients with age 44.4±5.4 years. Of the total patients, 55% had steatosis. Seven parameters were significantly associated with IR in CHC-4 patients including; body mass index (BMI), serum AST, TG, vaspin and leptin, and grades of steatosis and fibrosis. Logistic regression analysis identified TG (p=0.01) and fibrosis (p=0.001) as the independent predictors of IR. In multivariate analysis HCV viral load (p=0.03), vaspin (p=0.007), HOMA-IR (p=0.016), and HAI (p=0.009) were independently associated with grades of steatosis. The factors independently associated with liver fibrosis identified by multivariate analysis were BMI (p

      PubDate: Mon, 22 Jul 2013 09:31:18 GMT
       
  • Antibacterial activity of some natural Duhokian honey against clinical
           isolates of Staphylococcus aureus, Iraq

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Najim Abdulla yassin, Alaa Muyassar Ahmad, Adnan Abdulla Mohammed.
      Antibiotic resistance of bacteria is on the rise, namely multi-resistant Staphylococcus aureus which are resistant to all of the antibiotics in common use. Thus the discovery of alternative theuraptic agents is urgently needed. Honey is an ancient wound treatment that was re-introduced into modern medical practice and possesses therapeutic potential, including wound healing properties and antimicrobial activity. The aim of this study is to investigate and comparison of antibacterial activity of four types of natural honey, found in Duhok city, with commercial one against a multi-resistant Staphylococcus aureus strains isolated from wounds and burns infections in Duhok city, Iraq. Methods A total of 100 wound and burn swabs were collected and analyzed by screened on Blood, Mannitol salt, MacConkey and Nutrient agar followed by the identification of the isolates based on their cultural characteristics and their reactions in standard biochemical tests. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. The antibacterial activity of four natural honeys (agriculture college honey, Barwari Bala , Emadia and Shingal) and one commercial Al-Sheefa honey was evaluated by agar diffusion assay and determination of MIC values. Of the 100 swabs, 25 (25%) swab yielded positive culture of Staphylococcus aureus; the organism exhibited high resistance rates to all used antibiotics. The all natural honeys exhibited highest antibacterial activity than commercial one. Moreover, agriculture college honey had excellent antibacterial activity, at very low concentration 5% (wt/vt) produce inhibition zones, followed by Barwari Bala, Emadia then Sningal honey. Minimal inhibitory concentration (MIC) values of four types of natural honeys were found to be 5, 25, 50 and 50 %(v/v) for AC honey, Barwari Bala, Emadi and Shingal honey respectively. This study highlights that some natural Duhokian honey has complete and partial antibacterial activity when tested in vitro against a multi-resistant Staphylococcus aureus strains isolated from wounds and burns infections.

      PubDate: Sat, 20 Jul 2013 16:49:54 GMT
       
  • Histopathological examination of all resected gall bladder specimens
           – Requisite or redundant?

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Vani BR, Deepak Kumar B, Srinivasa Murthy V, Padma Priya Kasukurti.
      Unsuspected gall bladder carcinoma constitutes 0.3 to 2.85% of cholecystectomies. In the light of its poor prognosis and aggressive nature, the importance of incidentally diagnosed carcinoma cannot be highlighted enough. This study was undertaken to determine the prevalence of various gall bladder lesions in insured patients of a model hospital and highlighting the need of gallbladder resection. Histopathological study of cholecystectomy specimens was conducted over a period of 2 years. 229 were chronic cholecystits among which 177 were associated with calculi. 27 cases were cholesterolosis, in whom 16 had associated chronic inflammation in the wall induced by calculi. Two cases of adenocarcinoma were detected in clinically and grossly unsuspected cholecystitis cases with associated gall stones (mixed type). Histopathological detection of carcinoma is a gold standard and will continue to be superior to clinical and radiological examination.

      PubDate: Fri, 21 Jun 2013 02:23:16 GMT
       
  • Blood glucose levels in nephritic syndrome patients undergoing renal
           biopsy

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Ogu Carison, Kim Cheon.
      Nephrotic syndrome is classified as a disease associated with immune impairment. Nephrotic syndrome associated with other metabolic disorders is often manifested in the progression of diabetic nephropathy. In this study, the authors analyzed the prevalence of hyperglycemia in 316 patients suffering from nephrotic syndrome and who were undergoing renal biopsy. According to our study, 34 (10.8%) of the 316 subjects presented hyperglycemia fulfilling criteria for diabetes (fasting blood glucose> 126 mg/dL) and a considerable number of diabetic cases was detected . As intensive management of diabetic nephropathy has not been recorded, the combination of diabetes mellitus and nephrotic syndrome should be looked for in all cases with this syndrome.

      PubDate: Sun, 26 May 2013 23:20:20 GMT
       
  • Histopathological diagnosis of gastric biopsies in correlation with
           endoscopy – a study in a tertiary care centeR.

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Dr.Kirana Pailoor, Dr.Murali Keshava Sarpangala, Ramesh C.N Naik.
      This study was done to evaluate the histopathological diagnosis of gastric endoscopic biopsies and to correlate the endoscopic and histopathological diagnosis of these neoplastic and non-neoplastic gastric lesions. A total of fifty-two endoscopic biopsies were studied retrospectively. Biopsies were retrieved using flexible fibre – optic endoscope and also video-endoscope. They were transferred to a bottle containing 10% neutral formalin, processed and stained routinely with Haematoxylin and Eosin. Special stains such as Mucicarmine and Giemsa were done as and when required. Out of 52(100%) gastric endoscopic biopsies, 29(56%) were malignant and 23(44%) were benign. The correlation of endoscopic and histopathological diagnosis of these gastric lesions was 57.28%. The sensitivity was 65.96%, specificity 90.57%, positive predictive value 86.11% and negative predictive value was 75%. Endoscopic examination and biopsy is a convenient procedure for accurate objective assessment of patients with symptoms of gastrointestinal tract. Endoscopy is incomplete without biopsy and pathology is the gold standard for the diagnosis of endoscopically detected lesions.

      PubDate: Mon, 22 Apr 2013 22:46:42 GMT
       
  • Serum concentration of liver function indicators in HIV patients on
           different classes of highly active antiretroviral therapy (HAART)

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Idonije OB, Osadolor HB, Festus OO.
      Soon after the introduction of HAART , drug related toxicities became recognized and well characterized, thus liver toxicity has become a growing problem among HIV patients on HAART hence this study. Liver function was assessed in 290 HIV patients {80 on NNRTIs-based HAART, 80 on NRTIs-based HAART, 80 on PIs-based HAART and 50 newly diagnosed and not on any antiretroviral medication serving as test control} using plasma concentration of alanine transaminase (ALT) and aspartate transaminase (AST) as test indicators. Each of the three groups on HAART were further subdivided into four subgroups of 3, 6, 12 and>12 months based on the duration on HAART with each subgroup consisting of twenty patients. The ALT and AST concentration were determined using standard methods. When the patients on different classes of HAARTS were compared with the control group, the differences in enzyme (ALT and AST) activities were significantly higher in 3 and 6 months duration on HAART for all the three classes of HAART used. The NNRTIs and PIs-based groups also showed a significant difference only in ALT activity in the 12 months duration on HAART. When the ALT and AST activities of patients on NRTIs- based HAART group were compared with the NNRTIs and PIs- based HAART groups, statistically significant differences were observed only in the 3 and 6 months duration on HAART. Conclusively HAART has a duration and drug dependent effects on the liver cells integrity and functions. This effect is lesser with NRTIs as compared with NNRTIs and PIs. It is therefore recommended that routine liver function tests be instituted in HIV patients on HAART regimen.

      PubDate: Wed, 17 Apr 2013 07:08:00 GMT
       
  • Correlation between fever and leukocyte in dengue infection, a clinical
           observation

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Tuk Chea Sovannah, Vein Roun Haang.
      Dengue infection is a major public health problem, affecting general population in the Southeast Asia Region. The hematologic aberration including lymphocytosis, coagulopathy and predominant thrombocytopenia are the common manifestation in the patients with dengue infection. Here, the author reported the experience about fever and leukocyte count of 130 patients with dengue infection who visited the author during endemic season, 2012. The average level of leukocyte among these patients was studied. The correlation between the recorded body temperature of the patients and the leukocyte level was investigated. We found that the fever is common in the patient with dengue infection and the lymphocytosis, not leukocytosis is the common leukocyte disorder in these patients. The correlation between body temperature and total white blood cell count and differential white blood cell count is poor and not significant. Therefore, the fever and the leukocyte disorder in the patients with dengue fever is only a co - presentation.

      PubDate: Sun, 24 Mar 2013 00:55:05 GMT
       
  • In vitro effect of Ginseng, Ficus fovealata Wall. and Elephantopus scaber
           Linn. on spermatozoa

    • Abstract: Publication year: 2013
      Source:Advance Laboratory Medicine International
      Rakshmi Opas Singh, Kandhi Thamma Singh, Pisuth Gutta Prasad.
      Millions of world populations suffer from sexual drive dysfunction in their life. Despite the increasing availability of effective conventional medical treatments, alternative medicine, especially plant-derived and herbal remedies, continue to provide a popular alternative for men and women seeking to improve their sex life. Nevertheless, the efficacy of most herbal agents in treating sexual problems remains uncertain. Here, the author perform an in vitro test to study the effect of three herbs, which are widely used due to the belief that they can promote good sexual activity: Panax Ginseng, Ficus fovealata Wall., and Elephantopus scaber Linn. We found that the spermatic count and motility rate in the Ficus fovealata Wall., and Elephantopus scaber Linn. - added samples were lower than controls but the Ginseng – added were higher than the control.

      PubDate: Sun, 24 Mar 2013 00:33:13 GMT
       
 
 
JournalTOCs
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Email: journaltocs@hw.ac.uk
Tel: +00 44 (0)131 4513762
Fax: +00 44 (0)131 4513327
 
About JournalTOCs
API
Help
News (blog, publications)
JournalTOCs on Twitter   JournalTOCs on Facebook

JournalTOCs © 2009-2015