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Journal Cover   Advance Laboratory Medicine International
  [1 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 2228-8961 - ISSN (Online) 2228-8961
   Published by ScopeMed Homepage  [97 journals]
  • Evaluation of the flow cytometric osmotic fragility in the diagnostic of
           hereditary spherocytosis

    • Abstract: 2015-09-22T23-16-39Z
      Source: Advance Laboratory Medicine International
      Mohammed Nazim Bennaoum, Hassiba Lazreg, Affaf Adda.
      BHereditary spherocytosis is a constitutional hemolytic anemia due to the presence of spherical red cells in the blood secondary to a genetic defect of some membrane proteins. The test commonly used in the screening is the determination of the osmotic fragility. Practice this test is difficult to interpret and lack of sensitivity and specificity. The purpose of this study is to evaluate a new and simple method using flow cytometry for the determination of osmotic fragility. The principle of the test is to determine the percentage of residual red cells after the addition of deionised water which acts as an hemolysis-inducing agent. We therefore studied a group of 94 patients with 5 known cases with hereditary spherocytosis. Each sample was tested by osmotic globular resistance dilution analysis and by flow cytometry. The results are compared by PEARSON's correlation.The test has a sensitivity of 100% and a specificity of 96%. The correlation coefficient is -0.626 for the entire population (P

      PubDate: Tue, 22 Sep 2015 21:27:08 GMT
  • Groundnut oil as an alternative clearing agent to xylene in histological
           tissue processing

    • Abstract: 2015-08-14T04-21-26Z
      Source: Advance Laboratory Medicine International
      Ebenezer OLubunmi Esan, Ayodeji Blessing Ajileye, Samuel Ayobami Fasogbon.
      This research work was conducted to compare the tissue processing suitability of groundnut oil as a clearing agent with that of tissue processed with xylene as a clearing agent. Tissue samples were obtained from the liver, lungs, and small intestine of adult wistar rats. The tissue samples were processed with groundnut oil as a clearing agent and the other tissue samples which were also processed along with xylene as a clearing agent for a period of 4 hours to 24 hours using a manual tissue processing method. All the tissue samples processed with both groundnut oil and with xylene as a clearing agents were all sectioned with a rotary microtome and stained with Masson’s Trichrome and Haematoxylin and Eosin methods. Tissue samples labeled G1, G2 and G3, were cleared with Groundnut oil during tissue processing and these tissue samples were processed, but not as well as those tissue samples labeled X1, X2, X3, cleared with xylene during tissue processing. The tissue samples cleared and processed with Groundnut oil did not alter the histochemical staining of tissues. Simple staining techniques like Masson’s Trichrome and haematoxylin and Eosin were employed. The results showed that there was no difference between tissue sections cleared in Groundnut oil and those tissue sections cleared in Xylene. Groundnut oil, which is a cheap, easily available and non-toxic agent is suitable and can be used as a clearing agent in histological tissue processing.

      PubDate: Thu, 13 Aug 2015 04:33:58 GMT
  • Repeat endoscopy in Barrett's esophagus with no dysplasia and low-grade

    • Abstract: 2015-06-24T23-32-38Z
      Source: Advance Laboratory Medicine International
      Abdulla Mohammad Shendal.
      This letter to editor briefly discusses on the clinical advantage on performing Repeat endoscopy in Barrett's esophagus with no dysplasia and low-grade dysplasia.

      PubDate: Sun, 21 Jun 2015 21:05:26 GMT
  • Diagnostic error in health care: an issue that requires continuous concern

    • Abstract: 2015-05-27T14-20-54Z
      Source: Advance Laboratory Medicine International
      Theenar Hares Shokar.
      This letter to editor briefly discusses on the common problem, diagnostic errors in healthcare.

      PubDate: Mon, 25 May 2015 21:36:37 GMT
  • Correlation between fever and leukocyte in dengue infection, a clinical

    • Abstract: 2015-05-13T04-17-31Z
      Source: Advance Laboratory Medicine International
      Sophiank Vong, Arun Kumar Srininath, Rutheran Koff Rodoff.
      Dengue infection is a major public health problem, affecting general population in the Southeast Asia Region. The haematologic aberration including lymphocytosis, coagulopathy and predominant thrombocytopenia are the common manifestation in the patients with dengue infection. Here, the author reported the experience about fever and leukocyte count of 30 patients with dengue infection who visited the author during endemic season, 2013. The average level of leukocyte among these patients was studied. The correlation between the recorded body temperature of the patients and the leukocyte level was investigated. We found that the fever is common in the patient with dengue infection and the lymphocytosis, not leukocytosis is the common leukocyte disorder in these patients. The correlation between body temperature and total white blood cell count and differential white blood cell count is poor and not significant. Therefore, the fever and the leukocyte disorder in the patients with dengue fever is only a co - presentation.

      PubDate: Wed, 06 May 2015 00:40:58 GMT
  • Role of immunohistochemistry in differentiating muscularis mucosae and
           muscularis propria as an aid to accurate staging of invasive urothelial
           carcinoma: a pilot study

    • Abstract: 2015-02-23T09-31-21Z
      Source: Advance Laboratory Medicine International
      Prateek Kinra, Ajay Malik, Vibha Dutta.
      The common prognostic markers for urothelial neoplasms include- tumour grade, invasion into muscle, vascular invasion, histological type, lymph node status and currently molecular markers. However the single most important prognostic marker that has stood the test of time is the extent of invasion into muscularis propria. Treatment of bladder carcinoma is determined by the stage (depth of invasion). Distinguishing bladder muscularis propria from muscularis mucosae can be problematic especially in small transurethral resection specimens cut tangentially. Florid desmoplasia and hyperplastic muscularis mucosa is commonly mistaken for muscularis propria. The sections of 70 cases of urothelial carcinoma were subjected to immunohistochemistry by the three immunohistochemical stains [vimentin (for mesenchymal cells-myofibroblasts), Smooth Muscle Actin (for smooth muscle cells) and smoothelin (specific for muscularis propria)]. Our study showed a sensitivity of 98.23% & specificity of 98.13% for the combination of the three immunohistochemical markers used together in accurately assessing the invasion into muscularis propria. Smoothelin positive predictive value for muscularis propria was very high (96.49%) whereas very low (2.8%) for muscularis mucosa. Immunohistochemistry picked up 3/70 cases that were wrongly diagnosed as invasive (florid desmoplastic response was mistaken for muscularis propria layer). Vimentin showed a moderately high sensitivity and specificity (accuracy of 81.25%) for myofibroblasts in the neighbouring area around tumour thereby assisting in picking up desmoplasia. Immunohistochemistry panel composed of SMA, smoothelin, and vimentin should be used as per the algorithm suggested. This will aid in accurately labelling the muscularis propria and rule out false positive & false negative invasions.

      PubDate: Wed, 18 Feb 2015 03:53:44 GMT
  • Facial demodex infestation among HIV seropositive and seronegative

    • Abstract: 2015-01-29T09-34-48Z
      Source: Advance Laboratory Medicine International
      Beuy Joob, Chandrigra Singaraja.
      The skin disorder is usually an important problem in the patients with the problem of impaired immunity. The superficial dermatological problems can be seen in HIV infected patients but is not commonly mentioned in dermatology. In fact, in HIV infected cases, several problems can be seen such as onychomycosis, Tinea capitis, etc. Here, the authors report on the observation of facial demodex infestation, a rarely mentioned ectoparasitosis, among HIV seropositive and seronegative subjects. A small preliminary survey with improved transparent tape method was performed on 50 HIV seropositive and seronegative subjects. The prevalence rate of facial demodex infestation among HIV seropositive and seronegative subjects are equal 74 % and 46 %, respectively. Based on the present study, the similar prevalence of infestation among seronegative subjects to the previous publication on prevalence among healthy adults (44 %). Focusing on the prevalence among HIV infected patients, it is significantly higher than the rate among HIV seronegative group. This report is the pioneer work that addresses the importance of high prevalence of demodex infestation among HIV seropositive patients.

      PubDate: Thu, 29 Jan 2015 04:28:55 GMT
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