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Journal Cover Journal of Northeast Agricultural University
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     ISSN (Print) 1006-8104
     Published by Elsevier Homepage  [2571 journals]
  • Effect of Seed Soaking with Exogenous Proline on Seed Germination of Rice
           Under Salt Stress
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Liu Hua-long , Sha Han-jing , Wang Jing-guo , Liu Yang , Zou De-tang , Zhao Hong-wei
      To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy (GE), germination rate (GR), relative germination energy (RGE) and relative germination rate (RGR) significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol •L-1. Soaking with 15 mmol •L-1 and 30 mmol •L-1 proline significantly improved the amylase activities (e.g. alpha-amylase, beta-amylase and total amylase) of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol •L-1 proline brightened District I and increased the width of ‘i’ brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination.


      PubDate: 2014-11-08T12:18:59Z
       
  • High-throughput Sequencing Technology and Its Application
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Zhu Qiang-long , Liu Shi , Gao Peng , Luan Fei-shi
      Gene sequencing is a great way to interpret life, and high-throughput sequencing technology is a revolutionary technological innovation in gene sequencing researches. This technology is characterized by low cost and high-throughput data. Currently, high-throughput sequencing technology has been widely applied in multi-level researches on genomics, transcriptomics and epigenomics. And it has fundamentally changed the way we approach problems in basic and translational researches and created many new possibilities. This paper presented a general description of high-throughput sequencing technology and a comprehensive review of its application with plain, concisely and precisely. In order to help researchers finish their work faster and better, promote science amateurs and understand it easier and better.


      PubDate: 2014-11-08T12:18:59Z
       
  • Effect of Sodium Chloride on Meltability of Mozzarella Cheese
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Zhang Jian-qiang , Li Hao , Bian Chun , Cao Rong-an , Zhang Li-ping
      Meltability is one of the most important properties of Mozzarella cheese, as it is generally used in pizza and other foods. Mozzarella was prepared by no salted and immature production technology, and the effect of different addition amounts of salt on the meltability of mozzarella cheese was measured by Schreiber method and small amplitude oscillatory shear analysis (SAOSA) method. The results showed that different adding amounts of NaCl had significant influence on the meltability of Mozzarella cheese, and 2% NaCl addition was the best condition. The results measured by the methods of Schreiber and SAOSA were basically same: adding different amounts of NaCl had significant influence on the hardness and elasticity of mozzarella cheese, but no significant influence on the sticky. It was a good microscopic structure arrangement of Mozzarella cheese with 2% NaCl addition. Scanning electron micrographs showed that a space grid structure formed by casein had changed, and formed many uniform molecular holes. The results indicated that different addition amounts of salt had influence on meltability of no salted immature Mozzarella cheese, and this technology could be drastically shorten the processing time.


      PubDate: 2014-11-08T12:18:59Z
       
  • Technical Research for Detector of Grain Moisture Content Based on Error
           Compensation
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Dong Yu-de , Yang Xian-long , Ye Fei , Pan Kai , Jin Xing-chi , Shi De-cai
      According to the existing method including testing the frequency and establishing the relationship between moisture content and frequency, a corresponding instrument was designed. In order to further improve the accuracy and rapidity of the system, a new approach to describe the relationship between the measurement error and the temperature was proposed. The error band could be obtained and divided into several parts (based on the range of temperature) to indicate the error value that should compensate the grain moisture content for the changes in temperature. By calculating the error band at the maximum and the minimum operating temperatures, as well as by determining the error compensation value from the error band based on the measurement moisture content, the final effective result was derived.


      PubDate: 2014-11-08T12:18:59Z
       
  • Correlation Between Physicochemical Properties and Eating Qualities of
           Rice
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Qin Ke-xin , Liu Lin-lin , Liu Tian-yi , Cheng Wen-hong , Shi Yan-guo
      Correlation among physicochemical properties, which include amylose content, alkali spreading values, gel consistency, water absorption, expansion rate, solid content of rice-water, protein content and fat content, and eating qualities of six kinds of rice samples planted in Heilongjiang Province were studied. Correlation analysis showed that amylose content, water absorption and expansion rate were negatively correlated with eating qualities, yet gel consistency, alkali spreading values, solid content of rice-water and fat content were positively correlated with eating qualities. Among them, eating quality had an obvious correlation with amylose content and gel consistency, but no significant correlation with protein content. The regression equation, which described the relationship of the eating quality scores and physicochemical indexes, was Y=94.439–12.711X 1–23.721X 2–0.701X 3+0.570X 4+186.938X 5 (X 1, X 2, X 3, X 4 and X 5 represented amylose content, water absorption, expansion rate gel, consistency and fat content). The single factor analysis of variance showed that six kinds of rice existed significant differences in quality category.


      PubDate: 2014-11-08T12:18:59Z
       
  • Design and Implementation of Stud-farm Daily Management System Based on
           C/S Structure
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Luan Hong-liang , Wang Hong-bin , Qin Hong-yu , Wang Chao , Zhai Zhi-nan , Xiao Jian-hua
      In order to achieve the computerization and digitization of the daily system of stud-farm, the immunization system technology, racecourse health monitoring technologies and the stud-farm prevention technologies were combined to built the stud-farm daily management system. By using Visual Studio 2008 and Microsoft Office Access 2003, an immunization information system was developed based on Client/Server structure and combined with the key immunization link of horses. The system included stud-farm immune management and racecourse health monitoring system. The immune management contained immunization information input, the inquiry of immunization information, immunization statistics, immunization-reminding and system management. The racecourse health monitoring system included stud-farm monitoring, horses monitoring and inquiry and statistics of health monitoring. The software realized the analysis and tips of the daily information in stud-farm. The software could record the appropriate data and carry on the statistics. The stud-farm daily management system greatly improved the efficiency of the expanded program about immunization and horses’ healthy rearing technique.


      PubDate: 2014-11-08T12:18:59Z
       
  • Lentivirus Mediated Gene Manipulation in Trophectoderm of Porcine Embryos
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Yin Zhi , Guo Jia , Bou Gerelchimeg , Liu Shi-chao , Mu Yan-shuang , Liu Zhong-hua
      Development of tools that can manipulate gene expression specifically and efficiently in the trophectoderm (TE) lineage would greatly aid understanding the roles of different genetic pathways in TE versus embryonic lineages. Here, we showed first time that short-term lentivirus infection of porcine blastocysts could lead to rapid expression of transgene specifically in TE cells. Efficient TE-specific gene knockdown could also be achieved by lentivirus-mediated pol III-driven short hairpin RNA (shRNA) and TE-specific gene expression could be temporal controlled efficiently by combining this system with Tet-On system. This lentivirus lineage-specific infection system would facilitate gene function studies in porcine pre-implatation embryos by specifically knockdown or overexpression of these genes in TE.


      PubDate: 2014-11-08T12:18:59Z
       
  • Effects of Allicin on Lipid Metabolism and Antioxidant Activity in
           Chickens
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Wang Gong-chen , Han Lu-lu , Wang Jing , Lang Wan-nan , Pan Chuan-yi , Li Yan-fei
      To investigate the effects of allicin on chickens’ lipid and antioxidant performance, Hy-laying hens’ diets were replenished with 0 mg • kg-1, 50 mg • kg-1, 100 mg • kg-1, and 150 mg • kg-1 allicin for 42 days, respectively. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TCHO), high density lipoprotein (HDL), and low density lipoprotein (LDL) levels were measured in chicken serum. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) levels were measured in chicken serum and liver tissue homogenate. The results showed that the supplement dose of allicin tested did not significantly change the activity of ALT or AST (P>0.05); TG and CHOL levels decreased with the increase of allicin additive doses, and the difference between treatment groups and CG was significant (P<0.05), and there was the best effect with 100 mg • kg-1; allicin significantly reduced the content of MDA, and increased SOD and GSH-Px activities compared with CG (P<0.05), and 100 mg • kg-1 of allicin resulted in the strongest SOD and GSH-Px activity. The antioxidant function test results of liver tissue homogenate were consistant with that of serum. Our findings indicated that allicin could enhance antioxidant capacity and reduce blood lipid level in chickens and 100 mg • kg-1 was the optimal amount of allicin additives.


      PubDate: 2014-11-08T12:18:59Z
       
  • Transgenic Petunia hybrida with Silicon Transporter Protein OsLsi1 and
           OsLsi2 Genes and Its Drought Resistance Analysis
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Yang Tao , Zhao Ran , Zhao Jing , Wan Liang , Fan Jin-ping
      As one of the important materials in landscaping for flower terrace and border, Petunia hybrida needs high environmental conditions and its growth is seriously influenced by the drought. Silicon is considered to be a necessary element for plant growth, and soluble silicon can improve plant resilience. To improve the drought resilience of Petunia hybrida, the silicon transporter protein OsLsi1 and OsLsi2 genes cloned from rice (Oryza sative) were transferred into Petunia hybrida by Agrobacterium-mediated method, and finally got 26 and 32 positive plants, respectively by PCR and RT-PCR detections. With a control of non-transgenic plants, the obtained transgenic plants were taken by drought treatment stress for 0, 4, 7, 10 and 14 days, then re-watered and measured physiological indexes as malondialdehyde (MDA) content, free proline (Pro) content, superoxide dismutase (SOD) activity and peroxidase (POD) activity to study the effect of Petunia's drought resistance. All the results proved that the silicon transporter protein OsLsi1 and OsLsi2 genes were normally transcripted and expressed in transgenic Petunia hybrida; OsLsi1 gene could improve the abilities of plants’ drought resistance and recover after drought stress, while OsLsi2 gene could reduce the above abilities. The order of the drought resistance ability of the three strains from strong to weak was OsLsi1>CK>OsLsi2; and silicon indeed improved the ability of drought resistance as well. All these results provided a new way to improve the drought resistance of Petunia, and laid a foundation to improve the ability of garden plants’ drought resistance and water saving.


      PubDate: 2014-11-08T12:18:59Z
       
  • Method for Isolating Mitochondrial DNA from Etiolated Tissue of Cabbage
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Wang Shuai , Wang Chao , Zhang Xiao-xuan
      Isolation of high-quality mitochondrial DNA (mtDNA) is an important premise for researching molecular mechanisms in cytoplasmic male sterility of cabbage (Brassica oleracea L.var.capitata). An efficient protocol for separation and purification of mitochondria and extraction of mitochondrial DNA (mtDNA) from etiolated tissues of cabbage was developed. We took a method combined mannitol density gradient with differential centrifugation, selected appropriate rotational speed, extended DNase I treating time and changed mitochondria cracking condition. The results showed that the extracted mitochondria in this protocol had complete structure, appeared to ellipsoid and had not been contaminated with other impurities under the Jannus Green B staining. The isolated mitochondrial DNA had high purity and yield through detecting the optical density, nuclear specific primer PCR and agarose gel electrophoresis. The results indicated that mitochondrial DNA extracted by this protocol had high quality and enabled to be used in futher genetic studies.


      PubDate: 2014-11-08T12:18:59Z
       
  • Effects of Methylated Soybean Oil Adjuvant on Fomesafen Efficacy to Weeds
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Han Yu-jun , Fu Jiu-cai , Wang Qian-yu , Tao Bo , Mao Zi-jun
      Tank-mix adjuvant has the potential to improve the weed control efficacy of post-emergence herbicides. In order to study the synergistic effect of adjuvant, the effects of different rates of fomesafen alone or applied methylated soybean oil adjuvant (MSO) were sprayed on redroot pigweed, abutilon and black nightshade under greenhouse condition. The results showed that fomesafen had different performance on the three weeds, and MSO adjuvant could effectively increase the control. The nightshade control was lower than other two weeds with all the fomesafen doses from 131.25 to 506.25 ga.i. • hm-2 with or without adjuvant. The control of abutilon was between the black nightshade and the redroot pigweed, and had better control at 375 ga.i. • hm-2 with adjuvant or 506.25 ga.i. • hm-2 alone or with adjuvant respectively. The results indicated that mixing adjuvant with fomesafen improved the control on weeds, especially at the low rate. Black nightshade was more difficult to control. The redroot pigweed had the most susceptibility to fomesafen alone or with adjuvant.


      PubDate: 2014-11-08T12:18:59Z
       
  • Physiological Changes and Cold Tolerance of Three Camphor Species During
           Natural Winter Temperature Fluctuations
    • Abstract: Publication date: September 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 3
      Author(s): Wang Ning , Yuan Mei-li , Yao Fang
      The dynamic changes in the malondialdehyde (MDA), superoxide dismutase (SOD), soluble sugar, proline, and soluble protein contents, as well as the relative electrolyte conductivity and the corresponding cold resistance, of Cinnamomum bodinieri Level., C. camphora L., and C. caudiferum Kisterm were investigated during the winter months of October 2009 to April 2010. During the short period of temperature decline that lasted until mid-December, the changes in the relative electrolyte conductivity and MDA content with temperature were insignificant. In January, SOD activity continued to increase and then peaked as a result of rapid increases in soluble sugar, proline, soluble protein, as well as the inhibition of the relative electrolyte conductivity and decrease in MDA content. These physiological changes protected the camphor trees from cold damage during winter. From February to March, SOD activity and the soluble protein and proline contents increased with the increase in temperature. However, the relative electrolyte conductivity and MDA content decreased, indicating that the cell membrane damaged by low temperature was gradually being repaired. The cold dip in April led to slight increases in the relative electrolyte conductivity and MDA content. Using a fuzzy mathematics method, the cold resistance adaptability of the camphor trees was divided into three periods namely, the enhancement setting stage, the vigorous stage, and the reducing stage. The cold tolerance abilities were ranked as the following order: C. bodinieri Level>C. camphora L.>C. caudiferum Kisterm.


      PubDate: 2014-11-08T12:18:59Z
       
  • Analysis of Influential Factors on Agricultural Surplus Labor
           Professionalization During China's Economic Downturn
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Yang Xiu-li , Li Lu-tang
      This paper contributed to the pool of studies about agricultural surplus labor in China, also acted as the root to the imminent settlement of the issues concerning agriculture, countryside and farmers. Using data from survey of agricultural surplus labor in 2012, which covered three provinces in northern, midwestern and southern parts of China, this paper analyzed the influential factors on agricultural surplus labor professionalization by adoption of a logistic regression model. It showed that agricultural surplus labor shortage could be explained by low-quality professionalization. It was a feasible and effective way to solve the issue of workforce shortage during economic downturn by improving agricultural surplus labor's professionalization.


      PubDate: 2014-07-26T11:52:47Z
       
  • Role of miRNA in Mammary Gland Development and Lactation
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Li Qing-zhang , Wang Chun-mei , Gao Xue-jun
      miRNA can regulate development and milk yield of the mammary gland through epigenetic mechanism. miRNA can directly and indirectly modulate the activity of the epigenetic machinery, target genes through post-inhibition of translation initiation, mediate miRNA decay, target genes and inhibit the positive regulation, regulate tone modification, and regulate DNA methylation of target genes. Here we reviewed the role of miRNAs in mammary gland development and lactation. Researching miRNA in mammary gland development and lactation process, and understanding the response of the epigenetic mechanisms to external stimuli will be an important necessity to devise new technologies for maximizing their activity and milk production in the dairy cow.


      PubDate: 2014-07-26T11:52:47Z
       
  • Seasonal Variation of Major Elements in South Lake Cyohoha, Rwanda
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Jean de Dieu Bazimenyera , Fu Qiang , Théophila Niragire
      The paper analyzed the seasonal variation of the concentrations of Cr, Mn, Al, N, P, As, Ba, Ca, Cu, Fe, Mg, and K in South Lake Cyohoha water using spectroscopic technique. Water samples were taken monthly at Ngenda, Karehe and Nyamabuye stations from January 2009 to December 2010. The results showed that the concentrations of aluminum, nitrogen, potassium, arsenic, phosphorous, manganese, chromium, barium and copper were high during the raining season and low during the dry season, while calcium, iron and magnesium varied independently with seasonal change. The results of conductivity and pH also confirmed the effects of seasonal change on the quality of water in the South Lake Cyohoha since the highest value of conductivity was found during the raining season, while the smallest was observed during the dry season, for pH the highest number was noticed during the dry season and the lowest during the raining season.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Sequence Analysis of Y-box Binding Protein Gene in Min Pig
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Zhang Dong-jie , Liu Di , Wang Liang , He Xin-miao , Wang Wen-tao
      In order to study the gene sequence of Min pig Y-box binding protein (YB-1) gene, the complete coding sequence of Min pig YB-1 gene was cloned by RT-PCR, the sequence features were analyzed by some software and online website. The results showed that the complete CDS of Min pig Y-box was found to be 975 bp long, encoding 324 amino acids. It contained a conserved cold shock domain and several phosphorylation sites, but had no transmembrane domains, and was consistent with a protein found in the cytoplasm. Min pig YB-1 nucleotides shared high similarity (61.37%–97.66%) with other mammals.


      PubDate: 2014-07-26T11:52:47Z
       
  • Application of Protein Feed Processed by Microbial Fermentation to Dairy
           Cow
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Sun Zhe , Liu Ying , Pan Hong-bao , Gao Xue-jun
      Methionine (Met) and lysine (Lys) have been reported as the first two limiting amino acids (AA) for maximum milk yield and milk protein production. Supplying these AA may improve microbial protein synthesis and therefore improve milk production without adding excess N to the environment. This observation utilized fermented soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM) and corn by Bacillus subtilis 168 and Leuconostoc mesenteroides as core feedstuffs to produce special biological protein feed for dairy cow. The results showed that the milk production, milk protein percentage, milk fat percentage and milk DM percentage of test groups in trial period were significantly more than those of the control group (P<0.01), the results showed that adding fermenting protein feed in dairy cow diets could significantly improve milk yield, milk protein and milk fat content. The economic benefits of actual application were analyzed, the group of 0.5% was the best compared with the other groups.


      PubDate: 2014-07-26T11:52:47Z
       
  • Assessment of Phosphorus Levels in Small Ruminants as Affected by Summer
           and Winter
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Ansar Mahmood , Asma Hassan Dr. , Iram Maqsood , Tang Li-jie , Syed Mohsin Bukhari , Khalil Ur-Rehman , Shahla Andleeb
      An experiment was carried out to assess the phosphorus status of free grazing goats at Faisalabad, Pakistan. Samples were collected fortnightly during summer and winter seasons of 2010 from soil and plants. The highest (118±0.54 mg • kg−1) levels of feces phosphorus were recorded in lactating goats during winter and (9.87±0.99 mg • kg−1) in urine of male during winter. Similarly maximum (71.0±0.88 mg • kg−1) phosphorus concentration was observed in the plasma of lactating animals. Milk contained (31.0±0.36) mg • L−1 in winter while during the months of summer the highest values recorded in forages, soils, canal and tube well waters were (755±1.98) mg • kg−1, (785±4.98) mg • kg−1, (0.97±8.78) mg • L−1 and (4.12±0.55) mg • L−1, respectively. It was revealed from the current results that fecal matter, forage, milk, tube well and canal water contained lower amounts of P, while P levels in blood plasma was found within the critical limits. Therefore, phosphorus supplementations were required in the area under experimentation to meet the requirements of the animals for their normal growth.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effect of MSTN Propeptide and shRNA Co-expression Vector on Proliferation
           of Skeletal Muscle Satellite Cells
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Feng Lin-he , Wang Xin , Lu Ming , Tong Hui-li , Li Shu-feng , Yan Yun-qin
      Myostatin (MSTN) is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expression vector pcDNA3.1-Pro-MSTNshRNA, transfected it into muscle satellite cells by Liposome 2000, and detected cell proliferation changes by CCK-8 method and flow cytometry after 48 h. The expressions of P21 and CDK2 were detected by Western blot and real-time PCR. The results showed that the cell vitality of experimental groups significantly increased than that of the negative control, and cells in S phase also increased significantly (P<0.05). After knocked down MSTN gene, P21 expression decreased (P<0.05), but CDK2 gene expression increased (P<0.05). These results indicated that MSTN gene expression was associated with P21 and CDK2, the proliferation of skeletal muscle satellite cells could be promoted while MSTN was inhibited, which provided a theoretical basis for the study on transgenic cattle.


      PubDate: 2014-07-26T11:52:47Z
       
  • Vertical Migrating and Cluster Analysis of Soil Mesofauna at Dongying
           Halophytes Garden in Yellow River Delta
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): He Fu-xia , Xie Tong-yin , Xie Gui-lin , Fu Rong-shu
      For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta (YRD), Shandong Province. The results showed that the soil mesofauna tended to gather on soil surface in most samples at most times, but the vertical migrating greatly varied in different seasons or environment conditions. Acari was the dominant group. The index of diversity of the soil fauna was correlated with the index of evenness. The Acari's number of individuals infected other species and numbers. Dominant group-Acari made greater contribution to the result of cluster analysis, and there were significant differences between communities in different habitats by cluster analysis with both Bray-Curtis and Jaccard similarity coefficient.


      PubDate: 2014-07-26T11:52:47Z
       
  • Simulation of in situ Root Decomposition of Two Barley Cultivars
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Xu Jing-gang , Duan Xue-jiao , Nooralla Juma
      Root C and root-released C are closely related to soil organic matter content and mechanistic simulation modeling has proven to be useful for studying root and soil organic C dynamics in plant-soil ecosystems. A computer model was designed in this study to simulate the dynamics of root C and root released C decomposition in situ and the dynamics of different forms of C in soil under two barley cultivars (Abee and Samson). The results showed that on the 15th day, about 48% of the total 14C fixed in roots was respired for Abee and 42% for Samson. This indicated that the turnover rate of root 14C of Abee was higher than that of Samson. The percentage of water-soluble organic 14C, active microbial 14C and stable 14C over the total fixed 14C were not different between two barley cultivars. From the analysis of the model for two barley cultivars, the total 14C transformed into different soil pools (excluding CO2-C and root C pools) for the two barley cultivars was similar (26% for Abee and 25% for Samson), but the difference of 14C remaining in soil between the two barley cultivars was mainly because of the difference of 14C remaining in roots which have not been yet decomposed. Some of the information which could not be measured in the laboratory conditions was obtained in this study.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Expression Analysis of Mlo Gene from Pericallis hybrida B.
           Nord.
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Wang Jin-gang , Li Wei , Ren Hong-wei , Lv Yuan-da , Bai Ding , Xu Jing
      The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) and RACE. The result of sequence analysis indicated that Mlo gene from Pericallis hybrida B. Nord. contained about 1 296 bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlo gene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlo gene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.


      PubDate: 2014-07-26T11:52:47Z
       
  • Study on Relationship Between Cucumber Germplasm and Propamocarb Residue
           Using Subjective Rating Technique
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Wu Peng , Qin Zhi-wei , Zhou Xiu-yan , Wu Tao , Xin Ming , Guo Qian-qian
      Propamocarb (PM) residue in cucumber (Cucumis sativus L.) receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions (26 breeding lines and six cultivars) from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit (r=0.97) and leaf (r=0.94). In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher (P<0.01) PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cultivation of Chinese Farmers' Integrity
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Yang Xiu-li , Zhu Xue-jiao
      Farmers as the largest population group in China, their integrity levels impact the process of socialist modernization and construction of socialist harmonious society directly. Farmers’ credit construction is an important part of the construction of new socialist countryside. As the subject of construction of new socialist countryside, some farmers are weak in credit awareness. Low credibility of traditional culture, uncompleted credit system construction and farmers’ narrow thoughts led to their lacking of credit awareness. We should perfect the rural credit system, strengthen the construction of rural credit culture and enhance the construction of grass-roots credit administration, set integrity example model and take other measures to promote farmers’ awareness of the integrity.


      PubDate: 2014-07-26T11:52:47Z
       
  • Financing Problems in China's Rural Areas
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Li Mei-ni , Han Xue-ping
      Solving the financing problem in rural areas and improving the level of economic development in rural areas become important guarantees for the expansion of domestic demand. This research, through studying the current situation of rural financing, pointed out the main factors restricting the financing in rural areas, such as high risk credit, slow income growth, high financing cost and difficult mortgage and guaranteed loans. Based on those, the author worked out the objectives and direction of China's rural financing reform, from two aspects of path selection of reform and innovation and specific embodiments for adjusting the overall reconstruction, and put forward some relevant countermeasures.


      PubDate: 2014-07-26T11:52:47Z
       
  • Farmer Labor Service Export from Heilongjiang Province to Russia
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Xu Hong-zhuan , Wang Li-min , Feng Zhan-fu
      As contiguous area to Russia, Heilongjiang Province takes advantage of geography and resource superiority to promote the export of farmer labor services to Russia in recent years with trading cooperation between China and Russia. This paper briefly introduced the present situation of the export of farmer labor services from Heilongjiang Province to Russia. Under the background of Russian Far East economic development and development of “large agriculture” in Heilongjiang Province, Heilongjiang Province would continue to increase labor export to Russia. Then, the paper analyzed the problems of the export of farmer labor services and gave some proposals accordingly. At last, the result showed that well-organized and reasonable farmer labor export to Russia could play a role to the agricultural modernization and urbanization development.


      PubDate: 2014-07-26T11:52:47Z
       
  • Research Progress on Nitrogen Use and Plant Growth
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Cui Xin , Yan Qing-wei , Sun Jia-lin , Xiao Shuang , Xie Fu-chun , Chen Ya-jun
      The application of nitrogen (N) fertilizers in agriculture has been increasing dramatically since 1970s. However, the over-fertilization causes could cause environmental problems, as well as low N use efficiency (NUE). Promoting NUE in plants and minimizing the environmental impacts of N fertilizers had been the focus of the current research. We reviewed the importance of N, N metabolism and plant growth, plant N physiology and the molecular aspect of N metabolism in this paper. The future development of N use and NUE of plants was also discussed.


      PubDate: 2014-07-26T11:52:47Z
       
  • Synthesis and Identification of SG-BSA and SG-OVA
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Zhu Ze-yao , Zhan Chun-xia , Zhang Qi-zhong
      Hapten sulfaguanidine (SG) was coupled with carrier protein bovine serum albumin (BSA) to form a full antigen SG-BSA by diazotization and glutaraldehyde methods. Ovalbumin (OVA) was used as protein carrier to couple with Hapten SG by glutaraldehyde method. Then, the immunogen and the coating antigen were purified by dialysis and gel exclusion chromatography. The conjugated ratio of SG to BSA in artificial antigen was 5.3 (using diazotization method) and 6.5 (glutaraldehyde method), and the conjugated ratio of SG to OVA in coating antigen was 2.3 (glutaraldehyde method) by UV-visible spectrophotometer. The coupling was successful according to the analysis of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). BALB/c mice were immunized with the antigen (SG-BSA), and the titers of antiserum were tested to be 1 : 6 400 and 1 : 400 after three periods of immunities by indirect ELISA, which further identified the success of the synthesis of both immunogen SG-BSA and coating antigen SG-OVA.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Dietary Protein and Temperature on Growth and Flesh Quality of
           Songpu Mirror Carp
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Wang Chang-an , Xu Qi-you , Zhao Zhi-gang , Li Jin-nan , Wang Lian-sheng , Luo Liang
      The study was conducted to demonstrate the effects of dietary protein and water temperature on growth and flesh quality of Songpu mirror carp (Cyprinus carpio) at an initial weight (165.24±5.08) g. Practical diets were formulated to contain five protein levels (29.12%, 31.46%, 34.49%, 38.17%, and 40.13%), and each diet was randomly assigned triplicate groups of 15 fishes at three temperatures (18°C, 22°C, and 26°C) in the recirculation system. Fishes were fed twice daily to apparent satiation for 56 days. Results indicated that fishes had higher weight gain rate at 22°C and 26°C than that at 18°C (P<0.05), but there were no significant differences between 22°C and 26°C (P>0.05). Based on the weight gain rate, dietary protein level 29.12% could meet the requirement of the body at 18°C, 22°C, or 26°C. Crude protein, crude lipid, moisture and ash of muscle had no significant differences among those treatments (P>0.05). pH (after 24 h) of muscle was the highest at 18°C and the lowest at 22°C (P<0.05), but no differences were observed among different protein level groups at each temperature (P>0.05). No significant differences on shear force, water holding capacity, collagen, glycogen and lactate among all the treatments were found (P>0.05). It was concluded that when C. carpio fed to apparent satiation, the growth mainly depended upon temperature. Dietary protein could not significantly affect flesh quality, but temperature significantly affected pH of muscles. A dietary protein level 29.12% could meet the requirement of the body at 18°C, 22°C, or 26°C.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Maternal Dietary Energy Restriction on Fat Deposition of
           Offspring
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Li Jing-feng , Xu Liang-mei , Zhang Yan-yun , Jiang Dan , Zhang Jing , Zhang Hui , Li Jie
      The study was carried out to investigate the effects of maternal dietary energy restriction on growth performance, serum indices and fat deposition of offspring. A total of 400 female Arbor Acres (AA) broiler breeders were studied. These female birds involved three experimental treatments and a control group with normal dietary energy diets (ND, 11.7 MJ of ME • kg−1 during the laying). In treatments 2, 3 and 4, the energies of diets were 20%, 30% and 50% (LD20, LD30 and LD50) lower than those of the control, respectively. The study commenced at the beginning of the laying period when the total egg production reached 5% of the flock. All the broiler offspring were fed the same diets. The results showed that in low energy diets, offspring showed decreased 1-day-old weight, but 49-day-old weight was higher in LD20 diet (P<0.05). For offspring during days 1-49, the average daily gain (ADG) in LD20 group and the feed conversion ratio in LD50 group were improved as compared with those of the control (P<0.05). Compared with the control, abdominal fat percentage increased in 49-day-old offspring from LD30 diet (P<0.05); the fat content of breast muscle in offspring increased in broilers fed low energy diets (P<0.05). In 28-day-old offspring from breeders given LD20 and LD50 diets, liver fat percentages were higher compared with ND (P<0.05). The subcutaneous fat thickness in 28-day-old offspring from LD50 group and 49-day-old offspring from LD30 group was higher (P<0.05). On day 49, the serum cholesterol (CHO) of offspring from breeders fed LD20 diet and serum high-density lipoprotein (HDL) of offspring from breeders fed LD50 diet reduced compared with those of the control (P<0.05). In addition, a higher triiodothyronine (T3) content in serum was found in offspring from broiler breeders given LD20 and LD30 diets (P<0.05). Serum thyroxine (T4) in offspring significantly decreased with the decrease of diet energy (P<0.05). In conclusion, to a certain extent, dietary energy restriction in breeders could improve growth performance and promote lipid metabolism of offspring.


      PubDate: 2014-07-26T11:52:47Z
       
  • Expression of HSP72 in Mouse Preimplantation Embryos with Heat Shock
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Suo Jia-jia , Cao Rong-feng , Cui Xiao-ni , Jiang Zhong-ling , Cong Xia , Cui Kai , Tian Wen-ru
      The objective of the paper was to detect HSP72 expression and HSP72 gene sequence in heat shocked mouse preimplantation embryos and the effects of different thermo conditions on hatching rates of embryos. The mouse blastocysts cultured in vitro were heat treated at 40°C and 38°C for 1 h, 2 h and 3 h and then recovered at 37°C for 3 h, 2 h and 1 h, respectively, to detect their HSP72 gene expression by using RT-PCR after the total RNA extraction. The hatching rate of the blastocysts for different treated groups was recorded and the expression of HSP72 in the blastocysts was determined by Western blot. The results showed that all the groups of blastocysts, including the control, had the expression of HSP72 gene. The expression of HSP72 protein had the highest level in the embryos stressed at 38°C for 2 h, and it was significantly higher than that in the control group. The expression of HSP72 in the groups of blastocysts treated at 40°C was not significantly different from that in the control group. The embryos with induction of mild heat shock at 38°C for 2 h, then subjected to heat shock at 40°C for 2 h, had a significant higher (P<0.05) hatching rate of 54.74% compared to 47.85% in the embryos treated directly at 40°C for 2 h. The above results indicated that the mouse blastocysts were sensitive to heat shock and a mild heat shock induced HSP72 gene expression. Induction of HSP72 expression with mild heat shock helped embryos to tolerate more severe heat shocks.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Substitute Media on Development of Potted Cyclamen percicum
           Mill.
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Liu Qing-chao , Wang Kui-ling , Liu Qing-hua , Pan Hui-tang , Zhang Qi-xiang
      Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss (PM). The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir (PCC) were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.


      PubDate: 2014-07-26T11:52:47Z
       
  • Decursin Safety from Root of Angelica gigas in Rats
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Cheng-zhe Jiang , Jin-man Feng , Jian-feng Zhang , Ilhyun Han , Seyoung Choung
      The subacute intraperitoneal toxicity of decursin was investigated in Spargue-Dawly (SD) rats. The rats were treated with decursin at a dose of 125 and 250 mg · kg−1 · day−1 for 4 weeks. All animals were observed daily for clinical signs. Their body weights were recorded weekly, no mortality was observed in two doses of 125 and 250 mg · kg−1 of decursin. There were no significant differences in the clinical observations, body weight, food and water consumption, hematology, blood chemistry, gross pathological examination or organ weight between control and treated animals of both sexes. In conclusion, no evidence of a subacute toxic potential was observed in this study and no indication for adverse effects was noted at a dose level of 250 mg · kg−1 · day−1.


      PubDate: 2014-07-26T11:52:47Z
       
  • Recognition of Human G-protein Coupled Receptors Using Compressed Amino
           Acid Alphabets
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Cui-ping Guan
      G-protein coupled receptors (GPCRs) have a relatively conservative seven transmembrane helix (7tm) regions, and their N and C termini are various. In order to strengthen the features of GPCR families, N and C termini were removed in this study, then frequency features in the form of single amino acid and dipeptide compositions for recognition of human GPCRs were analyzed and extracted based on the compressed amino acid alphabets. Based on these features, classifiers were developed using support vector machine (SVM). The ability of different compressed methods was investigated. The testing results demonstrated that the suitable choice of compressed method combined with amino acid composition information could get good performance for the recognition of human GPCRs.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Probiotics on Growth, Pork Quality and Serum Metabolites in
           Growing-finishing Pigs
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Tian-yang Liu , Bin-chao Su , Jia-li Wang , Chao Zhang , An-shan Shan
      A total of 72 growing-finishing pigs (56 days old) were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups (four pens/group and six pigs/pen). Diet treatments were a corn-soybean meal (control group) and a corn-soybean meal with 1% probiotics (probiotics group) or 80 mg · kg−1 colistin sulfate (antibiotics group). Pigs were slaughtered when pigs of the lightest averaged 90 kg (157 days of age). There was no difference (p>0.05) in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups (p<0.05). Diet supplemented with probiotics reduced the drip loss and cooking loss of pork (p<0.05). Dietary supplementation with probiotics significantly decreased the content of triglycerides, low-density lipoprotein and urea nitrogen in serum (p<0.05), and increased the content of high-density lipoprotein in serum (p<0.05). These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.


      PubDate: 2014-07-26T11:52:47Z
       
  • Isolation of Nile Tilapia (Oreochromis niloticus) β-actin Promoter
           and Assay of Its Transcription Activity
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Mao-yuan Wang , Hong Yang , Quan-fu Zhong , Ming-yong Lai , Hai-ping Fan
      Through PCR amplification, 5′ flanking region and partial open reading frame (ORF) of gene of Nile tilapia (Oreochromis niloticus) was cloned by PCR amplification. Sequence analysis showed that no difference was found in known functional regions. This study was to construct and identify the mammalian expression vector of pEGFP-β-actin and to detect whether it could express in HEK 293T cell line. pEGFP-β-actin was transfected into HEK 293T cells with Lipofectamine 2000. The results showed that correct construction of recombinant pEGFP-β-actin has been shown by restriction enzyme digestion. The expression of gene in HEK 293T cells could be observed under microfluoroscope. pEGFP-β-actin could repress EGFP protein in HEK 293T cells. The results showed that β-actin gene promoter possessed effective transcription activities in eukaryotic cells. The work laid foundations for further study on the gene engineering and autotransgenic tilapia.


      PubDate: 2014-07-26T11:52:47Z
       
  • Seasonal Variation of Moisture Availability at Water-wind Erosion
           Crisscross Region in Northern Loess Plateau China
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Lu Tan , Zhong-bo Wang , Jin-bai Huang , Hiroshi Yasuda
      The objectives of the current study were to estimate evapotranspiration (ET) over the grassland and assess seasonal variation of moisture availability at the wind-water erosion crisscross region in the northern Loess Plateau of China. The Liudaogou Catchment which has the representative climatic and hydrological characteristics of the wind-water erosion crisscross region was chosen as the study location. The reference crop evapotranspiration (ET 0) was estimated by Penmen method, which was recommended by FAO56 and the evapotranspiration over the grassland (ET) was estimated by Penmen-Monteith equation using the observed meteorological data with time unit of 1 h. The soil moisture availability factor was defined by ma=ET/ET 0. The calculated results for 2006 indicated that the total ET 0 was slightly more than the total yearly precipitation and ET accounted for 37 % of that, ET increased distinctly after the intensive rainfall event in the rainy season. Most of the ma was less than 0.4 and its annual mean was 0.34. It was expected that the results provided a basis for studies on dynamic functional analysis of soil moisture, relationship between soil water and crop growth at the wind-water erosion crisscross region in the northern Loess Plateau.


      PubDate: 2014-07-26T11:52:47Z
       
  • Strategies for Promoting Rice Self-sufficiency in Sierra Leone
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Alphajoh Alhadi Bah
      Rice is the main food of Sierra Leoneans and an important source of employment and income for rural communities. According to the West African Rice Development Association report, annual consumption of rice is amongst the highest in Sub-Saharan Africa (SSA), 104 kg. Mainly, small scale farmers who are resource poor on both the upland and various lowlands grow rice. Sierra Leone has not been able to produce enough rice to meet its local consumption demand for a very long time now. 530 000 MT of milled rice is the required consumption need of the Sierra Leonean population per annum. In 2007, national paddy rice production was projected at 638 000 MT. The level of rice self-sufficiency rose from 50.57% in 2002 to 62.15% in 2006 and then to 63.49% in 2007. The remainder must be imported at increasingly expensive prices in the current situation of high food prices, which includes rice. The price of rice has seen a steady increase in the entire country. What most affected by this, is the low-income urban and peri-urban households. The promotion of domestic rice production is therefore a key element in the strategies for improving rice self-sufficiency, stimulating economic growth and increasing rural income. Therefore, supporting rice production programme is the only solution to pull the country out of the worsening rice situation and maintain the present trend in rice self-sufficiency. Therefore, having an understanding of the strategic interventions in rice production in Sierra Leone is an important ingredient in the promotion and attainment of rice self-sufficiency.


      PubDate: 2014-07-26T11:52:47Z
       
  • Constraints on Problems of Rural Surplus Manpower Capital Transfer in
           China
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Ying Chen , Yu-you Zou , Yao Chen , Hai-long Shen
      In recent years, researches on rural human capital are of great concern, but for how to effectively use the rural human capital is scanty, and thus, the transfer of rural human capital becomes a kind of way to solve rural human capital utilization. A portion of China rural human capital has been successful transferred, however, because of the restrain of their conditions, they can only be engaged in some low level work. In China rural areas, there are a lot of rural human capitals who are not successful transferred. How to successfully transfer the rest of this part rural human capital and how to make them play roles will be an important task. In this paper, according to the practical situations, we first defined the rural human capital, and then briefly summarized the importance of human capital transfer. At last, we found out the restriction factors of rural human capital transfer in China, and put forward some suggestions and measures to solve the problems.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of CMV Enhancer on Activity and Specificity of Bovine MyoG Gene
           Promoter
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Xin Wang , Ming Lu , Lin-he Feng , Yun-qin Yan
      Connected a segment of CMV enhancer to the front of MyoG gene promoter and then constructed the corresponding dual luciferase expression vector pGL3-CMV-MyoGpro. We set four eukaryotic expression vectors including pGL3-CMV, pGL3-MyoGpro, pGL3-CMV-MyoGpro, and pGL3-Basic which contained CMV promoter, MyoG promoter, CMV-MyoG synthesis promoter, and a promoterless negative control, respectively. Then the four vectors and internal control Renilla luciferase report gene vector phRL-TK were transfected into bovine skeletal muscle satellite cells, mouse C2C12 cells and bovine fetal fibroblast cells to detect the promoter activity with dual luciferase report system. The results showed that CMV enhancer could significantly improve the transcription activity of bovine MyoG gene promoter in muscle satellite cells and mouse C2C12 cells, and it had certain specificity. This study provided experimental materials for increasing the high expression of exogenous gene in bovine muscle cells, and also laid the molecular theoretical basis for obtaining the high specific promoter of bovine muscle and the transgenic beef cattle.


      PubDate: 2014-07-26T11:52:47Z
       
  • Expression of Six bHLH Superfamily Members in Mouse Uterus During Early
           Pregnancy
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Yong-wang Li , Xiao-ming Hou , Hua Ni
      bHLH transcript factor superfamily members involve in various bio-events in mice. According to existing researches, there are six members of bHLH superfamily likely to participate in the uterine development during early pregnancy in mice. Expression of six members of bHLH superfamily Hes6, Tcf23, Twist1, Twist2, Mitf and Tead4 in mouse uterus in early pregnancy was detected by RT-qPCR. All the six genes were expressed in early pregnancy mouse uterus, and expression pattern of each one was different from others. In situ hybridization results showed that Tead4 was expressed in decidual tissues of the 8th day pregnancy mouse. Tead4 might be involved in the regulation of embryo implantation in mice.


      PubDate: 2014-07-26T11:52:47Z
       
  • Galactopoietic Activity of Dibutyl Phthalate Isolated from Vaccaria
           segetalis
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Hui-li Tong , Xue-jun Gao , Zun-lai Sheng , Qing-zhang Li , Shu-feng Li , Nan Li , Jie Liu , Yun-qin Yan
      A galactopoietic compound, identified as dibutyl phthalate (DBP), was isolated from Vaccaria segetalis. The activity of DBP on lactation ability of dairy cow mammary gland epithelial cells (DCMECs) cultured in vitro and dairy cow was evaluated. Results showed that DBP could promote cell viability, proliferation ability, lactose and β-casein secretion of DCMECs, which could also raise the milk yields of dairy cows significantly.


      PubDate: 2014-07-26T11:52:47Z
       
  • Comparison of Physiological Properties Between Dwarf and Vine-type
           Cucumbers (Cucumis sativus Linn.)
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Xin Ming , Qin Zhi-wei , Zhou Xui-yan , Wu Tao
      Dwarfism is one of the most important traits in crop breeding. In this study, a dwarf cucumber D0462 and a vine NA129 were taken as experimental materials. The metabolism of hydrogen peroxide (H2O2) and superoxide anion radical (O2 −•), activities of antioxidant enzymes, and the levels of endogenous hormones of the two cultivars were compared. In the whole growth period, D0462 maintained higher H2O2 and O2 −• levels than NA129. The activities of SOD (superoxide dismutase), CAT (catalase), APX (ascorbate peroxidase) and IAAO (indoleacetic acid oxidase) in D0462 were found higher than those in NA129. The contents of gibberellic acid (GA3), indole-3-acetic acid (IAA), zeatin riboside (ZR) and jasmonic acid (JA) in internode were determined by enzyme-linked immunosorbent assay (ELISA). The internode of D0462 remained lower levels of GA3, IAA, and ZR. In contrast, JA level in internode of D0462 was higher than that of NA129. Compared with NA129, the cell mumbers in internode of D0462 did not change, but cell size evidently decreased. The chloroplasts in mature mesophyll D0462 exhibited swollen filled with starch grains instead of regular oval ones, and thylakoids of chloroplast swelled and became ambiguous.


      PubDate: 2014-07-26T11:52:47Z
       
  • Identification of Powdery Mildew Pathogen and Ribosomal DNA-ITS Sequence
           Analysis on Melon
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Xue-zheng Wang , Bing-yin Xu , Wang Ping , Fei-shi Luan , Hong-yan Ma , Ying-yuan Ma
      Identification of powdery mildew pathogens on melon (Cucumis melo) is important for melon breeding and disease-resistant germplasm selection. In this study, a powdery mildew pathogen that infected melon plants in Heilongjiang Province, China, was investigated in terms of host identification, morphological characteristics and phylogenetic relationships. The morphological characteristics of the pathogen were observed at five phases in the life cycle: germinating conidia, primary germ tube, hyphae, conidiophores, and colonization. The conidia were elliptical, colorless, catenulate, and the average length was 29.07 µm and average width was 17.82 µm. One ascus and eight ascospores were produced. DNA was extracted from 0.01 g conidiophores from a strain of powdery mildew pathogen that infected melon. ITS ribosomal DNA region (524 bp) was amplified with the universal ITS1 and ITS4 primers. The nucleotide sequence showed 100% similarity with ITS sequences for three Podosphaera fusca strains obtained from the GenBank database. The identity of the pathogen was confirmed as Sphaerotheca fuliginea. International standard differential hosts were used to identify S. fuliginea strain as 2F race. These results supported the notion that Podosphaera fusca was a synonym of S. fuliginea.


      PubDate: 2014-07-26T11:52:47Z
       
  • Bioinformatic Analysis of Functional Characteristics of miR172 Family in
           Tomato
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Jie Li , Yu-shi Luan , Jun-miao Zhai , Ping Liu , Xiu-ying Xia
      MicroRNAs, a class of endogenous non-coding small RNAs about 21 nucleotides in length, play pivotal roles in plant physiological and biochemical processes. Tomato is important economic crop throughout the world. Recently, involvement of miRNAs in tomato has received significant attention. MiR172 is one of the conserved miRNAs in tomato. Investigation into the roles andthe target genes of this small RNA molecular in Arabidopsis illustrated that miR172 functions in regulating the transitions between developmental stages and in specifying floral organ identify. Herein, we combined bioinformatics and molecular biology approaches to profile the functional characteristics of miR172 in tomato. The results of target prediction showed that AP2-like ethylene-responsive transcription factors were major targets of miR172, analysis of upstream sequence indicated the presence of stress-related cis-elements in its promoter regions, and further analysis of qRT-PCR confirmed that miR172 participated in various stress responses. Our research provided a paradigm for further in-depth investigation of the functional characteristics of miRNAs in tomato.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Expression Analysis of Sucrose Non-fermenting-1 Related
           
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Hong-mei He , Zhi-wei Qin , Zhuo Feng , Tao Wu
      The sucrose non-fermenting-1 related protein kinase (SnRK), whose expression is induced by kinds of hyperosmotic stresses, plays a key role in improving stress resistance of plants. In order to investigate the molecular mechanism of low nitrogen resistance in cucumber, the full-length cDNA of SnRK gene was cloned in this study. The result showed that SnRK gene was 1 548 bp in length, encoded 515 amino acids, and had more than 80% homology with other crops. The protein encoded by this gene was an unstable and hydrophilic protein with no transmembrane structure and no signal peptide. Under nitrogen-free conditions and low nitrogen conditions, the expression pattern analysis of SnRK gene showed that this gene was up-regulated and its expression increased and was significantly higher than the normal level as the nitrogen concentration decreased. In addition, the expression of SnRK gene was also inhibited in the high nitrogen level and was significantly lower than the normal level. The result of this study would help us understand the molecular mechanism of low nitrogen resistance in cucumber.


      PubDate: 2014-07-26T11:52:47Z
       
  • Types of Maize Virus Diseases and Progress in Virus Identification
           Techniques in China
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Cui Yu , Zhang Ai-hong , Ren Ai-jun , Miao Hong-qin
      There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, maize crimson leaf disease, maize wallaby ear disease and corn lethal necrosis disease. This paper reviewed their occurrence and distribution as well as virus identification techniques in order to provide a basis for virus identification and diagnosis in corn production.


      PubDate: 2014-07-26T11:52:47Z
       
  • Biogenesis of Plant MicroRNAs
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Kong Wen-wen , Wang Hong-bo , Li Jing
      microRNAs (miRNAs) play important regulatory roles in eukaryotic gene expression, predominantly at the posttranscriptional level. Elaborate and diverse biogenesis pathways have evolved to produce miRNAs. miRNA biogenesis is a multistep process including transcription, precursor slicing, methylation, nuclear export, and RNA-induced silencing complex assembly. In the decade, since the first discovery of plant miRNAs, many enzymes and regulatory proteins involved in miRNA biogenesis in plants have been uncovered and a basic picture of miRNA processing is emerging gradually. In this article, we summarized the current study of plant miRNA biogenesis and discussed the multiple integrated steps and diverse pathways of miRNA processing.


      PubDate: 2014-07-26T11:52:47Z
       
  • Regulation of Foliar Application DCPTA on Growth and Development of Maize
           Seedling Leaves in Heilongjiang Province
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Gu Wan-rong , Meng Yao , Zhang Jun-bao , Ji Biao , Wang Yong-chao , Li Jing , Wei Shi
      DCPTA (2-diethylaminoethyl-3, 4-dichlorophenylether) is a new plant regulator which can be used to regulate growth and development for crops. Experiments on maize seedlings were conducted in the growth chamber to study the effects of foliar applied DCPTA. The plant pots were placed in a completely randomized design with three replicates. The maize seedlings were treated with 0 mg • L−1 (control), 20 mg • L−1 and 40 mg • L−1 DCPTA solution. The effects of DCPTA on the photosynthetic characteristics (photosynthesis, stomata conductance, intercellular CO2, and transpiration rate), related physiological characteristics (contents of soluble sugar and starch), chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm, Fv/Fo, qP, and qN) and the weight of dry matter in maize seedling were studied. The results showed that DCPTA enhanced photosynthesis of maize seedling. In general, photosynthetic rate in leaves was significantly promoted through spraying DCPTA solution, and 40 mg • L−1 DCPTA was found to be the best concentration for maize. The relationship between stomata conductance and transpiration rate in maize leaves could be described as linear. With regard to the chlorophyll fluorescence parameters, our findings showed that 40 mg • L−1 DCPTA in maize seedling caused an increase in Fm, Fv/Fm, Fm/Fo, qP and a decrease in Fo and qN at some time points checked. It is suggested that DCPTA increased photosynthetic rate by raising both the content of chlorophyll and activities of PSII and the contents of sugar and starch. Compared with the control, the treated maize seedling caused an increase in plant height, root length, shoot dry mass, root dry mass, or the total (root plus shoot) dry mass.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of CuSO4 and Uniconazole on Mature Embryo Culture in Japonica Rice
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Shan He , Zou Deng-tang
      In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10–15 μmol • L−1 CuSO4 and 0.50–1.00 mg • L−1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol • L−1. When the concentration of CuSO4 was 15 μmol • L−1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol • L−1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg • L−1 and for Longjing 24 was 1.00 mg • L−1.


      PubDate: 2014-07-26T11:52:47Z
       
 
 
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