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Journal Cover Journal of Northeast Agricultural University
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     ISSN (Print) 1006-8104
     Published by Elsevier Homepage  [2563 journals]
  • Analysis of Influential Factors on Agricultural Surplus Labor
           Professionalization During China's Economic Downturn
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Yang Xiu-li , Li Lu-tang
      This paper contributed to the pool of studies about agricultural surplus labor in China, also acted as the root to the imminent settlement of the issues concerning agriculture, countryside and farmers. Using data from survey of agricultural surplus labor in 2012, which covered three provinces in northern, midwestern and southern parts of China, this paper analyzed the influential factors on agricultural surplus labor professionalization by adoption of a logistic regression model. It showed that agricultural surplus labor shortage could be explained by low-quality professionalization. It was a feasible and effective way to solve the issue of workforce shortage during economic downturn by improving agricultural surplus labor's professionalization.


      PubDate: 2014-07-26T11:52:47Z
       
  • Role of miRNA in Mammary Gland Development and Lactation
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Li Qing-zhang , Wang Chun-mei , Gao Xue-jun
      miRNA can regulate development and milk yield of the mammary gland through epigenetic mechanism. miRNA can directly and indirectly modulate the activity of the epigenetic machinery, target genes through post-inhibition of translation initiation, mediate miRNA decay, target genes and inhibit the positive regulation, regulate tone modification, and regulate DNA methylation of target genes. Here we reviewed the role of miRNAs in mammary gland development and lactation. Researching miRNA in mammary gland development and lactation process, and understanding the response of the epigenetic mechanisms to external stimuli will be an important necessity to devise new technologies for maximizing their activity and milk production in the dairy cow.


      PubDate: 2014-07-26T11:52:47Z
       
  • Seasonal Variation of Major Elements in South Lake Cyohoha, Rwanda
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Jean de Dieu Bazimenyera , Fu Qiang , Théophila Niragire
      The paper analyzed the seasonal variation of the concentrations of Cr, Mn, Al, N, P, As, Ba, Ca, Cu, Fe, Mg, and K in South Lake Cyohoha water using spectroscopic technique. Water samples were taken monthly at Ngenda, Karehe and Nyamabuye stations from January 2009 to December 2010. The results showed that the concentrations of aluminum, nitrogen, potassium, arsenic, phosphorous, manganese, chromium, barium and copper were high during the raining season and low during the dry season, while calcium, iron and magnesium varied independently with seasonal change. The results of conductivity and pH also confirmed the effects of seasonal change on the quality of water in the South Lake Cyohoha since the highest value of conductivity was found during the raining season, while the smallest was observed during the dry season, for pH the highest number was noticed during the dry season and the lowest during the raining season.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Sequence Analysis of Y-box Binding Protein Gene in Min Pig
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Zhang Dong-jie , Liu Di , Wang Liang , He Xin-miao , Wang Wen-tao
      In order to study the gene sequence of Min pig Y-box binding protein (YB-1) gene, the complete coding sequence of Min pig YB-1 gene was cloned by RT-PCR, the sequence features were analyzed by some software and online website. The results showed that the complete CDS of Min pig Y-box was found to be 975 bp long, encoding 324 amino acids. It contained a conserved cold shock domain and several phosphorylation sites, but had no transmembrane domains, and was consistent with a protein found in the cytoplasm. Min pig YB-1 nucleotides shared high similarity (61.37%–97.66%) with other mammals.


      PubDate: 2014-07-26T11:52:47Z
       
  • Application of Protein Feed Processed by Microbial Fermentation to Dairy
           Cow
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Sun Zhe , Liu Ying , Pan Hong-bao , Gao Xue-jun
      Methionine (Met) and lysine (Lys) have been reported as the first two limiting amino acids (AA) for maximum milk yield and milk protein production. Supplying these AA may improve microbial protein synthesis and therefore improve milk production without adding excess N to the environment. This observation utilized fermented soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM) and corn by Bacillus subtilis 168 and Leuconostoc mesenteroides as core feedstuffs to produce special biological protein feed for dairy cow. The results showed that the milk production, milk protein percentage, milk fat percentage and milk DM percentage of test groups in trial period were significantly more than those of the control group (P<0.01), the results showed that adding fermenting protein feed in dairy cow diets could significantly improve milk yield, milk protein and milk fat content. The economic benefits of actual application were analyzed, the group of 0.5% was the best compared with the other groups.


      PubDate: 2014-07-26T11:52:47Z
       
  • Assessment of Phosphorus Levels in Small Ruminants as Affected by Summer
           and Winter
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Ansar Mahmood , Asma Hassan Dr. , Iram Maqsood , Tang Li-jie , Syed Mohsin Bukhari , Khalil Ur-Rehman , Shahla Andleeb
      An experiment was carried out to assess the phosphorus status of free grazing goats at Faisalabad, Pakistan. Samples were collected fortnightly during summer and winter seasons of 2010 from soil and plants. The highest (118±0.54 mg • kg−1) levels of feces phosphorus were recorded in lactating goats during winter and (9.87±0.99 mg • kg−1) in urine of male during winter. Similarly maximum (71.0±0.88 mg • kg−1) phosphorus concentration was observed in the plasma of lactating animals. Milk contained (31.0±0.36) mg • L−1 in winter while during the months of summer the highest values recorded in forages, soils, canal and tube well waters were (755±1.98) mg • kg−1, (785±4.98) mg • kg−1, (0.97±8.78) mg • L−1 and (4.12±0.55) mg • L−1, respectively. It was revealed from the current results that fecal matter, forage, milk, tube well and canal water contained lower amounts of P, while P levels in blood plasma was found within the critical limits. Therefore, phosphorus supplementations were required in the area under experimentation to meet the requirements of the animals for their normal growth.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effect of MSTN Propeptide and shRNA Co-expression Vector on Proliferation
           of Skeletal Muscle Satellite Cells
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Feng Lin-he , Wang Xin , Lu Ming , Tong Hui-li , Li Shu-feng , Yan Yun-qin
      Myostatin (MSTN) is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expression vector pcDNA3.1-Pro-MSTNshRNA, transfected it into muscle satellite cells by Liposome 2000, and detected cell proliferation changes by CCK-8 method and flow cytometry after 48 h. The expressions of P21 and CDK2 were detected by Western blot and real-time PCR. The results showed that the cell vitality of experimental groups significantly increased than that of the negative control, and cells in S phase also increased significantly (P<0.05). After knocked down MSTN gene, P21 expression decreased (P<0.05), but CDK2 gene expression increased (P<0.05). These results indicated that MSTN gene expression was associated with P21 and CDK2, the proliferation of skeletal muscle satellite cells could be promoted while MSTN was inhibited, which provided a theoretical basis for the study on transgenic cattle.


      PubDate: 2014-07-26T11:52:47Z
       
  • Vertical Migrating and Cluster Analysis of Soil Mesofauna at Dongying
           Halophytes Garden in Yellow River Delta
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): He Fu-xia , Xie Tong-yin , Xie Gui-lin , Fu Rong-shu
      For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta (YRD), Shandong Province. The results showed that the soil mesofauna tended to gather on soil surface in most samples at most times, but the vertical migrating greatly varied in different seasons or environment conditions. Acari was the dominant group. The index of diversity of the soil fauna was correlated with the index of evenness. The Acari's number of individuals infected other species and numbers. Dominant group-Acari made greater contribution to the result of cluster analysis, and there were significant differences between communities in different habitats by cluster analysis with both Bray-Curtis and Jaccard similarity coefficient.


      PubDate: 2014-07-26T11:52:47Z
       
  • Simulation of in situ Root Decomposition of Two Barley Cultivars
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Xu Jing-gang , Duan Xue-jiao , Nooralla Juma
      Root C and root-released C are closely related to soil organic matter content and mechanistic simulation modeling has proven to be useful for studying root and soil organic C dynamics in plant-soil ecosystems. A computer model was designed in this study to simulate the dynamics of root C and root released C decomposition in situ and the dynamics of different forms of C in soil under two barley cultivars (Abee and Samson). The results showed that on the 15th day, about 48% of the total 14C fixed in roots was respired for Abee and 42% for Samson. This indicated that the turnover rate of root 14C of Abee was higher than that of Samson. The percentage of water-soluble organic 14C, active microbial 14C and stable 14C over the total fixed 14C were not different between two barley cultivars. From the analysis of the model for two barley cultivars, the total 14C transformed into different soil pools (excluding CO2-C and root C pools) for the two barley cultivars was similar (26% for Abee and 25% for Samson), but the difference of 14C remaining in soil between the two barley cultivars was mainly because of the difference of 14C remaining in roots which have not been yet decomposed. Some of the information which could not be measured in the laboratory conditions was obtained in this study.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Expression Analysis of Mlo Gene from Pericallis hybrida B.
           Nord.
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Wang Jin-gang , Li Wei , Ren Hong-wei , Lv Yuan-da , Bai Ding , Xu Jing
      The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) and RACE. The result of sequence analysis indicated that Mlo gene from Pericallis hybrida B. Nord. contained about 1 296 bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlo gene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlo gene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.


      PubDate: 2014-07-26T11:52:47Z
       
  • Study on Relationship Between Cucumber Germplasm and Propamocarb Residue
           Using Subjective Rating Technique
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Wu Peng , Qin Zhi-wei , Zhou Xiu-yan , Wu Tao , Xin Ming , Guo Qian-qian
      Propamocarb (PM) residue in cucumber (Cucumis sativus L.) receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions (26 breeding lines and six cultivars) from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit (r=0.97) and leaf (r=0.94). In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher (P<0.01) PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cultivation of Chinese Farmers' Integrity
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Yang Xiu-li , Zhu Xue-jiao
      Farmers as the largest population group in China, their integrity levels impact the process of socialist modernization and construction of socialist harmonious society directly. Farmers’ credit construction is an important part of the construction of new socialist countryside. As the subject of construction of new socialist countryside, some farmers are weak in credit awareness. Low credibility of traditional culture, uncompleted credit system construction and farmers’ narrow thoughts led to their lacking of credit awareness. We should perfect the rural credit system, strengthen the construction of rural credit culture and enhance the construction of grass-roots credit administration, set integrity example model and take other measures to promote farmers’ awareness of the integrity.


      PubDate: 2014-07-26T11:52:47Z
       
  • Financing Problems in China's Rural Areas
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Li Mei-ni , Han Xue-ping
      Solving the financing problem in rural areas and improving the level of economic development in rural areas become important guarantees for the expansion of domestic demand. This research, through studying the current situation of rural financing, pointed out the main factors restricting the financing in rural areas, such as high risk credit, slow income growth, high financing cost and difficult mortgage and guaranteed loans. Based on those, the author worked out the objectives and direction of China's rural financing reform, from two aspects of path selection of reform and innovation and specific embodiments for adjusting the overall reconstruction, and put forward some relevant countermeasures.


      PubDate: 2014-07-26T11:52:47Z
       
  • Farmer Labor Service Export from Heilongjiang Province to Russia
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Xu Hong-zhuan , Wang Li-min , Feng Zhan-fu
      As contiguous area to Russia, Heilongjiang Province takes advantage of geography and resource superiority to promote the export of farmer labor services to Russia in recent years with trading cooperation between China and Russia. This paper briefly introduced the present situation of the export of farmer labor services from Heilongjiang Province to Russia. Under the background of Russian Far East economic development and development of “large agriculture” in Heilongjiang Province, Heilongjiang Province would continue to increase labor export to Russia. Then, the paper analyzed the problems of the export of farmer labor services and gave some proposals accordingly. At last, the result showed that well-organized and reasonable farmer labor export to Russia could play a role to the agricultural modernization and urbanization development.


      PubDate: 2014-07-26T11:52:47Z
       
  • Research Progress on Nitrogen Use and Plant Growth
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Cui Xin , Yan Qing-wei , Sun Jia-lin , Xiao Shuang , Xie Fu-chun , Chen Ya-jun
      The application of nitrogen (N) fertilizers in agriculture has been increasing dramatically since 1970s. However, the over-fertilization causes could cause environmental problems, as well as low N use efficiency (NUE). Promoting NUE in plants and minimizing the environmental impacts of N fertilizers had been the focus of the current research. We reviewed the importance of N, N metabolism and plant growth, plant N physiology and the molecular aspect of N metabolism in this paper. The future development of N use and NUE of plants was also discussed.


      PubDate: 2014-07-26T11:52:47Z
       
  • Synthesis and Identification of SG-BSA and SG-OVA
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Zhu Ze-yao , Zhan Chun-xia , Zhang Qi-zhong
      Hapten sulfaguanidine (SG) was coupled with carrier protein bovine serum albumin (BSA) to form a full antigen SG-BSA by diazotization and glutaraldehyde methods. Ovalbumin (OVA) was used as protein carrier to couple with Hapten SG by glutaraldehyde method. Then, the immunogen and the coating antigen were purified by dialysis and gel exclusion chromatography. The conjugated ratio of SG to BSA in artificial antigen was 5.3 (using diazotization method) and 6.5 (glutaraldehyde method), and the conjugated ratio of SG to OVA in coating antigen was 2.3 (glutaraldehyde method) by UV-visible spectrophotometer. The coupling was successful according to the analysis of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). BALB/c mice were immunized with the antigen (SG-BSA), and the titers of antiserum were tested to be 1 : 6 400 and 1 : 400 after three periods of immunities by indirect ELISA, which further identified the success of the synthesis of both immunogen SG-BSA and coating antigen SG-OVA.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Dietary Protein and Temperature on Growth and Flesh Quality of
           Songpu Mirror Carp
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Wang Chang-an , Xu Qi-you , Zhao Zhi-gang , Li Jin-nan , Wang Lian-sheng , Luo Liang
      The study was conducted to demonstrate the effects of dietary protein and water temperature on growth and flesh quality of Songpu mirror carp (Cyprinus carpio) at an initial weight (165.24±5.08) g. Practical diets were formulated to contain five protein levels (29.12%, 31.46%, 34.49%, 38.17%, and 40.13%), and each diet was randomly assigned triplicate groups of 15 fishes at three temperatures (18°C, 22°C, and 26°C) in the recirculation system. Fishes were fed twice daily to apparent satiation for 56 days. Results indicated that fishes had higher weight gain rate at 22°C and 26°C than that at 18°C (P<0.05), but there were no significant differences between 22°C and 26°C (P>0.05). Based on the weight gain rate, dietary protein level 29.12% could meet the requirement of the body at 18°C, 22°C, or 26°C. Crude protein, crude lipid, moisture and ash of muscle had no significant differences among those treatments (P>0.05). pH (after 24 h) of muscle was the highest at 18°C and the lowest at 22°C (P<0.05), but no differences were observed among different protein level groups at each temperature (P>0.05). No significant differences on shear force, water holding capacity, collagen, glycogen and lactate among all the treatments were found (P>0.05). It was concluded that when C. carpio fed to apparent satiation, the growth mainly depended upon temperature. Dietary protein could not significantly affect flesh quality, but temperature significantly affected pH of muscles. A dietary protein level 29.12% could meet the requirement of the body at 18°C, 22°C, or 26°C.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Maternal Dietary Energy Restriction on Fat Deposition of
           Offspring
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Li Jing-feng , Xu Liang-mei , Zhang Yan-yun , Jiang Dan , Zhang Jing , Zhang Hui , Li Jie
      The study was carried out to investigate the effects of maternal dietary energy restriction on growth performance, serum indices and fat deposition of offspring. A total of 400 female Arbor Acres (AA) broiler breeders were studied. These female birds involved three experimental treatments and a control group with normal dietary energy diets (ND, 11.7 MJ of ME • kg−1 during the laying). In treatments 2, 3 and 4, the energies of diets were 20%, 30% and 50% (LD20, LD30 and LD50) lower than those of the control, respectively. The study commenced at the beginning of the laying period when the total egg production reached 5% of the flock. All the broiler offspring were fed the same diets. The results showed that in low energy diets, offspring showed decreased 1-day-old weight, but 49-day-old weight was higher in LD20 diet (P<0.05). For offspring during days 1-49, the average daily gain (ADG) in LD20 group and the feed conversion ratio in LD50 group were improved as compared with those of the control (P<0.05). Compared with the control, abdominal fat percentage increased in 49-day-old offspring from LD30 diet (P<0.05); the fat content of breast muscle in offspring increased in broilers fed low energy diets (P<0.05). In 28-day-old offspring from breeders given LD20 and LD50 diets, liver fat percentages were higher compared with ND (P<0.05). The subcutaneous fat thickness in 28-day-old offspring from LD50 group and 49-day-old offspring from LD30 group was higher (P<0.05). On day 49, the serum cholesterol (CHO) of offspring from breeders fed LD20 diet and serum high-density lipoprotein (HDL) of offspring from breeders fed LD50 diet reduced compared with those of the control (P<0.05). In addition, a higher triiodothyronine (T3) content in serum was found in offspring from broiler breeders given LD20 and LD30 diets (P<0.05). Serum thyroxine (T4) in offspring significantly decreased with the decrease of diet energy (P<0.05). In conclusion, to a certain extent, dietary energy restriction in breeders could improve growth performance and promote lipid metabolism of offspring.


      PubDate: 2014-07-26T11:52:47Z
       
  • Expression of HSP72 in Mouse Preimplantation Embryos with Heat Shock
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Suo Jia-jia , Cao Rong-feng , Cui Xiao-ni , Jiang Zhong-ling , Cong Xia , Cui Kai , Tian Wen-ru
      The objective of the paper was to detect HSP72 expression and HSP72 gene sequence in heat shocked mouse preimplantation embryos and the effects of different thermo conditions on hatching rates of embryos. The mouse blastocysts cultured in vitro were heat treated at 40°C and 38°C for 1 h, 2 h and 3 h and then recovered at 37°C for 3 h, 2 h and 1 h, respectively, to detect their HSP72 gene expression by using RT-PCR after the total RNA extraction. The hatching rate of the blastocysts for different treated groups was recorded and the expression of HSP72 in the blastocysts was determined by Western blot. The results showed that all the groups of blastocysts, including the control, had the expression of HSP72 gene. The expression of HSP72 protein had the highest level in the embryos stressed at 38°C for 2 h, and it was significantly higher than that in the control group. The expression of HSP72 in the groups of blastocysts treated at 40°C was not significantly different from that in the control group. The embryos with induction of mild heat shock at 38°C for 2 h, then subjected to heat shock at 40°C for 2 h, had a significant higher (P<0.05) hatching rate of 54.74% compared to 47.85% in the embryos treated directly at 40°C for 2 h. The above results indicated that the mouse blastocysts were sensitive to heat shock and a mild heat shock induced HSP72 gene expression. Induction of HSP72 expression with mild heat shock helped embryos to tolerate more severe heat shocks.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Substitute Media on Development of Potted Cyclamen percicum
           Mill.
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Liu Qing-chao , Wang Kui-ling , Liu Qing-hua , Pan Hui-tang , Zhang Qi-xiang
      Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss (PM). The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir (PCC) were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.


      PubDate: 2014-07-26T11:52:47Z
       
  • Decursin Safety from Root of Angelica gigas in Rats
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Cheng-zhe Jiang , Jin-man Feng , Jian-feng Zhang , Ilhyun Han , Seyoung Choung
      The subacute intraperitoneal toxicity of decursin was investigated in Spargue-Dawly (SD) rats. The rats were treated with decursin at a dose of 125 and 250 mg · kg−1 · day−1 for 4 weeks. All animals were observed daily for clinical signs. Their body weights were recorded weekly, no mortality was observed in two doses of 125 and 250 mg · kg−1 of decursin. There were no significant differences in the clinical observations, body weight, food and water consumption, hematology, blood chemistry, gross pathological examination or organ weight between control and treated animals of both sexes. In conclusion, no evidence of a subacute toxic potential was observed in this study and no indication for adverse effects was noted at a dose level of 250 mg · kg−1 · day−1.


      PubDate: 2014-07-26T11:52:47Z
       
  • Recognition of Human G-protein Coupled Receptors Using Compressed Amino
           Acid Alphabets
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Cui-ping Guan
      G-protein coupled receptors (GPCRs) have a relatively conservative seven transmembrane helix (7tm) regions, and their N and C termini are various. In order to strengthen the features of GPCR families, N and C termini were removed in this study, then frequency features in the form of single amino acid and dipeptide compositions for recognition of human GPCRs were analyzed and extracted based on the compressed amino acid alphabets. Based on these features, classifiers were developed using support vector machine (SVM). The ability of different compressed methods was investigated. The testing results demonstrated that the suitable choice of compressed method combined with amino acid composition information could get good performance for the recognition of human GPCRs.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of Probiotics on Growth, Pork Quality and Serum Metabolites in
           Growing-finishing Pigs
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Tian-yang Liu , Bin-chao Su , Jia-li Wang , Chao Zhang , An-shan Shan
      A total of 72 growing-finishing pigs (56 days old) were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups (four pens/group and six pigs/pen). Diet treatments were a corn-soybean meal (control group) and a corn-soybean meal with 1% probiotics (probiotics group) or 80 mg · kg−1 colistin sulfate (antibiotics group). Pigs were slaughtered when pigs of the lightest averaged 90 kg (157 days of age). There was no difference (p>0.05) in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups (p<0.05). Diet supplemented with probiotics reduced the drip loss and cooking loss of pork (p<0.05). Dietary supplementation with probiotics significantly decreased the content of triglycerides, low-density lipoprotein and urea nitrogen in serum (p<0.05), and increased the content of high-density lipoprotein in serum (p<0.05). These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.


      PubDate: 2014-07-26T11:52:47Z
       
  • Isolation of Nile Tilapia (Oreochromis niloticus) β-actin Promoter
           and Assay of Its Transcription Activity
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Mao-yuan Wang , Hong Yang , Quan-fu Zhong , Ming-yong Lai , Hai-ping Fan
      Through PCR amplification, 5′ flanking region and partial open reading frame (ORF) of gene of Nile tilapia (Oreochromis niloticus) was cloned by PCR amplification. Sequence analysis showed that no difference was found in known functional regions. This study was to construct and identify the mammalian expression vector of pEGFP-β-actin and to detect whether it could express in HEK 293T cell line. pEGFP-β-actin was transfected into HEK 293T cells with Lipofectamine 2000. The results showed that correct construction of recombinant pEGFP-β-actin has been shown by restriction enzyme digestion. The expression of gene in HEK 293T cells could be observed under microfluoroscope. pEGFP-β-actin could repress EGFP protein in HEK 293T cells. The results showed that β-actin gene promoter possessed effective transcription activities in eukaryotic cells. The work laid foundations for further study on the gene engineering and autotransgenic tilapia.


      PubDate: 2014-07-26T11:52:47Z
       
  • Seasonal Variation of Moisture Availability at Water-wind Erosion
           Crisscross Region in Northern Loess Plateau China
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Lu Tan , Zhong-bo Wang , Jin-bai Huang , Hiroshi Yasuda
      The objectives of the current study were to estimate evapotranspiration (ET) over the grassland and assess seasonal variation of moisture availability at the wind-water erosion crisscross region in the northern Loess Plateau of China. The Liudaogou Catchment which has the representative climatic and hydrological characteristics of the wind-water erosion crisscross region was chosen as the study location. The reference crop evapotranspiration (ET 0) was estimated by Penmen method, which was recommended by FAO56 and the evapotranspiration over the grassland (ET) was estimated by Penmen-Monteith equation using the observed meteorological data with time unit of 1 h. The soil moisture availability factor was defined by ma=ET/ET 0. The calculated results for 2006 indicated that the total ET 0 was slightly more than the total yearly precipitation and ET accounted for 37 % of that, ET increased distinctly after the intensive rainfall event in the rainy season. Most of the ma was less than 0.4 and its annual mean was 0.34. It was expected that the results provided a basis for studies on dynamic functional analysis of soil moisture, relationship between soil water and crop growth at the wind-water erosion crisscross region in the northern Loess Plateau.


      PubDate: 2014-07-26T11:52:47Z
       
  • Strategies for Promoting Rice Self-sufficiency in Sierra Leone
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Alphajoh Alhadi Bah
      Rice is the main food of Sierra Leoneans and an important source of employment and income for rural communities. According to the West African Rice Development Association report, annual consumption of rice is amongst the highest in Sub-Saharan Africa (SSA), 104 kg. Mainly, small scale farmers who are resource poor on both the upland and various lowlands grow rice. Sierra Leone has not been able to produce enough rice to meet its local consumption demand for a very long time now. 530 000 MT of milled rice is the required consumption need of the Sierra Leonean population per annum. In 2007, national paddy rice production was projected at 638 000 MT. The level of rice self-sufficiency rose from 50.57% in 2002 to 62.15% in 2006 and then to 63.49% in 2007. The remainder must be imported at increasingly expensive prices in the current situation of high food prices, which includes rice. The price of rice has seen a steady increase in the entire country. What most affected by this, is the low-income urban and peri-urban households. The promotion of domestic rice production is therefore a key element in the strategies for improving rice self-sufficiency, stimulating economic growth and increasing rural income. Therefore, supporting rice production programme is the only solution to pull the country out of the worsening rice situation and maintain the present trend in rice self-sufficiency. Therefore, having an understanding of the strategic interventions in rice production in Sierra Leone is an important ingredient in the promotion and attainment of rice self-sufficiency.


      PubDate: 2014-07-26T11:52:47Z
       
  • Constraints on Problems of Rural Surplus Manpower Capital Transfer in
           China
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Ying Chen , Yu-you Zou , Yao Chen , Hai-long Shen
      In recent years, researches on rural human capital are of great concern, but for how to effectively use the rural human capital is scanty, and thus, the transfer of rural human capital becomes a kind of way to solve rural human capital utilization. A portion of China rural human capital has been successful transferred, however, because of the restrain of their conditions, they can only be engaged in some low level work. In China rural areas, there are a lot of rural human capitals who are not successful transferred. How to successfully transfer the rest of this part rural human capital and how to make them play roles will be an important task. In this paper, according to the practical situations, we first defined the rural human capital, and then briefly summarized the importance of human capital transfer. At last, we found out the restriction factors of rural human capital transfer in China, and put forward some suggestions and measures to solve the problems.


      PubDate: 2014-07-26T11:52:47Z
       
  • Influence of Low-temperature Stress on Photosynthetic Traits in Maize
           Seedlings
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Zhuang Wen-feng , Wu Xin-juan , Yang Meng , Gu Wan-rong , Xu Zheng-jin , Li Jing
      Three varieties were employed as materials to study changes of photosynthetic traits under low-temperature stress. The results showed that Pn, Gs and Tr decreased under low-temperature treatment. Ci decreased under low-temperature treatment 18°C/9°C, and 16°C/7°C, and it decreased in earlier stage after increased under 14°C/5°C. WUE was increased in earlier stage and after stabilized. The order of the three varieties of cold resistance were Jinyu 5>Xingken 3>Jidan 198. They could make self-regulation through adjusting Gs, Tr, Ci and WUE.


      PubDate: 2014-07-26T11:52:47Z
       
  • Nutrient Status and Dynamics of Trifolium spp. at Different Growing Stages
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Zheng Hai-xia , Li Xue , Wang Yan-bin , Yin Xiu-jie , Yan Qin-wei , Chen Ya-jun
      The nutrient status and dynamics of three clover (Trifolium spp.) varieties at different growth stages were investigated with the crude protein content as an indicator of nutrient equilibrium. The crude protein, neutral detergent fiber (NDF), phosphorus (P), and calcium (Ca) content followed a decreasing trend at the initial growing stage and increased later. NDF and Ca content peaked in May, while crude protein and P content reached the highest in May and the lowest in July. The crude ash content followed an uncertain pattern as plants growing. The ash content of both wild-type red clover (T. pratensis L.) and ‘Sativum’ decreased at the beginning and then increased. However, the ash content of ‘Revendel’ white clover (T. repens L.) decreased all through the experimental period. The general trend was observed for all three clover varieties as the ash content was the highest at vegetative growing stage (May), and the acidic detergent fiber (ADF) content increased with time. The nutrient equilibrium value was ranked as ‘Revendel‘>wild-type red clover>‘Sativum‘.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Sequence Analysis of Actin Gene Fragment from Iris lactea
           var.chinensis Fisch.Koidz
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Fu Guo-hua , Yang Tao , Li Wei , Wang Jin-gang
      Degenerate primers were designed based on the conserved sequences of the Actin gene from other plants. Total RNA was extracted from the leaves of Iris lacteal var.chinensis Fisch.Koidz. Actin gene fragment was obtained by reverse transcription polymerase chain reaction (RT-PCR) and cloned into pMD18-T vector. The positive clone identified by PCR was sequenced. The sequencing result showed that the Actin gene fragment from Iris lacteal var.chinensis Fisch.Koidz contained about 598 bp, encoding 199 amino acids. Homology comparison with Actin gene sequences of other plants in the GenBank showed that it shared over 82% nucleotide sequence homology and 90% amino acid sequence homology. It indicated that this was the Actin gene. Because of the stability expression of Actin gene, it usually cited as the internal reference to study the expression and regulation of foundation in other genes of Iris lacteal var.chinensis Fisch.Koidz well.


      PubDate: 2014-07-26T11:52:47Z
       
  • Expression of an Episomal Vector in Developing Chicken Embryo and Chicks
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Mu Yan-shuang , Wang Jia-qiang , Yin Zhi , Jiang Dan-dan , Li Hui , Liu Zhong-hua
      The domesticated chicken has important roles in basic and applied research. The vector based on scaffold matrix attachment region (S/MAR) appears to be sufficient to maintain long-term expression in an episomal state in various mammalian cells. To explore the practical use of episomal vector in transgene technology of agricultural chickens, we fused the S/MAR of the human β-interferon gene into the pEGFP vector and transduced it into chickens by sperm-mediated gene transfer (SMGT). PCR detection indicated the positive rate of transgene chickens was 60%. The RT-PCR detection and fluorescence observation confirmed the expression of the GFP and indicated the existence of the GFP during the chicken embryogenesis and fetal development. The PCR detection and rescue experiments confirmed the episomal state of the pEPI-EGFP in chick embryos and chicks. These results showed that the S/MAR-based vector could function properly in chicken embryos and was a practicable tool combined with the SMGT to study the development of chickens.


      PubDate: 2014-07-26T11:52:47Z
       
  • Northern Blot and Immunohistochemistry Analysis of HMOX1 Expression in
           Blue- and Brown-shelled Chickens
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Wang Zhe-peng , Liu Rui-fang , Hua Peng-huan , Wang An-ru
      HMOX1 is an important functional candidate gene for chicken blue egg in view of its role in biosynthesis of biliverdin for blue egg coloration. To elucidate molecular mechanism of blue egg formation, this study detected expression of HMOX1 in blue-shelled chickens and brown-shelled chickens. Expression and alternative splicing of HMOX1 were detected by Northern blot, expression traits of HO-1 protein in shell glands of blue- (n=4) and brown-shelled (n=4) chickens were analyzed by immunohistochemistry. 3’ UTR of HMOX1 was cloned using 3'RACE. Results showed that the expression of HMOX1 at mRNA level had no significant difference between two groups of chickens, but at protein level HO-1 protein was highly expressed in blue-shelled chickens. Immunohistochemistry analysis showed that HO-1 protein expression was predominately located in villus epithelial cell of shell gland. Length of HMOX1 3’ UTR were 586 bp. In 3’ UTR we found a SNP of rs13866562 showing significant association with blue egg phenotype. Further miRNA prediction showed that it might influence interaction of some miRNAs and target sequences. The data suggested that blue egg is relevant to high expression of HO-1 in villus epithelial cell of shell gland. Further experimental validation for biological relevance of miRNAs is dispensable to elucidate reason for differential expressions of HO-1 protein.


      PubDate: 2014-07-26T11:52:47Z
       
  • Proteomic Analysis of Nuclear Phosphorylated Proteins in Dairy Cow Mammary
           Epithelial Cells Treated with Prolactin
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Huang Jian-guo , Lu Li-min , Qiao Bin , Liu Rong , Wang Jia-li , Pan Hong-bao , Li Qing-zhang , Gao Xue-jun
      Prolactin (PRL) is a versatile signaling molecule and regulates a variety of physiological processes, including mammary gland growth and differentiation and the synthesis of milk proteins. While PRL is known to be necessary for high levels of milk protein expression, the mechanism by which the synthesis of milk proteins is stimulated at the transcript level is less known. A major modification in the transcript level is protein phosphorylation. To gain additional insights into the molecular mechanisms at the transcript level underlying PRL action on the dairy cow mammary epithelial cells (DCMECs), nuclear phosphoproteins whose expression distinguishes proliferating regulated by PRL in DCMECs were identified. A phosphoprotein-enriched fraction from nuclear proteins was obtained by affinity chromatography, and a two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization time of matrix-assisted laser desorption/ionization/time of flight mass spectrometry (MALDI-TOF MS) were used to identify the changes of nuclear phosphoproteins in DCMECs treated with prolactin. Seven proteins displaying≥2-fold difference in abundance upon PRL treatment in DCMECs were identified by MALDI-TOF MS. The protein-GARS (GlyRS), which belonged to the class-II aminoacyl-tRNA synthetase family, played a global role in the milk protein synthesis. SERPINH1 (Heat shock protein 47), which was the first heat shock protein found to be a member of the serpin superfamily, regulated physiologic functions, such as complement activation, programmed cell death, and inflammatory processes. PRDX3, which belonged to a family of antioxidant enzymes, played an important role in scavenging intracellular reactive oxygen species (ROS). ACTR1A, belonged to the actin family, which was associated with transport of p53 to the nucleus. Annexin A2, a Ca2+-dependent phospholipid-binding protein, maintained the viability and cell cycle regulation of DCMECs. PSMB2 and PSMD10, which belonged to ubiquitin-proteasome system, were involved in several cellular processes, including cell cycle control, cellular stress response, intracellular signaling. This screening revealed that prolactin influenced the level of nuclear phosphoproteins in DCMECs. This result opens new avenues for the study of the molecular mechanism linked to the synthesis of milk proteins.


      PubDate: 2014-07-26T11:52:47Z
       
  • Construction and Screening of Bovine Bax Gene shRNA Interference
           Expression Vector
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Lu Ming , Wang Xin , Feng Lin-he , Yan Yun-qin
      Bax is a pro-apoptotic member of the Bcl-2 family genes which regulate programmed cell death. To decrease the apoptosis of bovine fibroblast cells, we construsted specific short hairpin (shRNA) expression vector encoding shRNA targeting Bax gene to screen the most effective vector. Four shRNAs sequences based on the sequence of bovine Bax mRNA in the GenBank were designed, and one scrambled shRNA sequence was regarded as negative control. The designed and synthesised single-stranded primer were annealed to double-stranded oligo sequences and cloned into linear pRI-GFP vector digested by enzymes Xho I and Bgl II. Screening positive cloning after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing. Named the correct vectors as pRI-GFP-Bax-190, pRI-GFP-Bax-206, pRI-GFP-Bax-215, pRI -GFP-Bax-389, pRI-GFP-Bax-NC (the negative control) and seleced them by quantitative PCR after transfected after 24 h and 48 h. The results showed that pRI-GFP-Bax-190 was the highest efficiency (95.47%), and significant difference (P>0. 01) after 48 h transfection. RNA interference (RNAi) mediated by shRNA expression vector could significantly down-regulate the expression of Bax gene in bovine fibroblast cells, which laid a foundation for further research.


      PubDate: 2014-07-26T11:52:47Z
       
  • Genetic Diversity Analysis Among Populations of Mink from China by
           Inter-simple Sequence Repeat (ISSR) Markers
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Yang Hong-yan , Fu Jing , Ning Fang-yong , Du Zhi-heng , Bai Xiu-juan
      Inter-simple Sequence Repeat (ISSR) analysis was applied to assess the genetic diversity within and among five populations of mink from Liaoning Province. A total of 20 primers were screened, five selected primers produced 35 discernible bands, with 30 (85.71%) being polymorphic, indicating high genetic diversity at the species level. The highest genetic diversity was observed in the brown mink population, whereas the lowest diversity was found in the standard-pitchy mink population. Based on genetic distance (1972), a dendrogram was constructed by using UPGMA algorithm, and five populations were divided into two major groups. Group I consisted of only the standard-pitchy mink population, and Group II included other four populations, in Group II, sapphire mink was close to brown mink population. The results of genetic differentiation indicated that the genetic differentiation degree between populations was lower and the genetic variation primarily came from within populations. This paper showed that ISSR technique was a reliable tool that could be used to study genetic diversity in the mink.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of CMV Enhancer on Activity and Specificity of Bovine MyoG Gene
           Promoter
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Xin Wang , Ming Lu , Lin-he Feng , Yun-qin Yan
      Connected a segment of CMV enhancer to the front of MyoG gene promoter and then constructed the corresponding dual luciferase expression vector pGL3-CMV-MyoGpro. We set four eukaryotic expression vectors including pGL3-CMV, pGL3-MyoGpro, pGL3-CMV-MyoGpro, and pGL3-Basic which contained CMV promoter, MyoG promoter, CMV-MyoG synthesis promoter, and a promoterless negative control, respectively. Then the four vectors and internal control Renilla luciferase report gene vector phRL-TK were transfected into bovine skeletal muscle satellite cells, mouse C2C12 cells and bovine fetal fibroblast cells to detect the promoter activity with dual luciferase report system. The results showed that CMV enhancer could significantly improve the transcription activity of bovine MyoG gene promoter in muscle satellite cells and mouse C2C12 cells, and it had certain specificity. This study provided experimental materials for increasing the high expression of exogenous gene in bovine muscle cells, and also laid the molecular theoretical basis for obtaining the high specific promoter of bovine muscle and the transgenic beef cattle.


      PubDate: 2014-07-26T11:52:47Z
       
  • Expression of Six bHLH Superfamily Members in Mouse Uterus During Early
           Pregnancy
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Yong-wang Li , Xiao-ming Hou , Hua Ni
      bHLH transcript factor superfamily members involve in various bio-events in mice. According to existing researches, there are six members of bHLH superfamily likely to participate in the uterine development during early pregnancy in mice. Expression of six members of bHLH superfamily Hes6, Tcf23, Twist1, Twist2, Mitf and Tead4 in mouse uterus in early pregnancy was detected by RT-qPCR. All the six genes were expressed in early pregnancy mouse uterus, and expression pattern of each one was different from others. In situ hybridization results showed that Tead4 was expressed in decidual tissues of the 8th day pregnancy mouse. Tead4 might be involved in the regulation of embryo implantation in mice.


      PubDate: 2014-07-26T11:52:47Z
       
  • Galactopoietic Activity of Dibutyl Phthalate Isolated from Vaccaria
           segetalis
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Hui-li Tong , Xue-jun Gao , Zun-lai Sheng , Qing-zhang Li , Shu-feng Li , Nan Li , Jie Liu , Yun-qin Yan
      A galactopoietic compound, identified as dibutyl phthalate (DBP), was isolated from Vaccaria segetalis. The activity of DBP on lactation ability of dairy cow mammary gland epithelial cells (DCMECs) cultured in vitro and dairy cow was evaluated. Results showed that DBP could promote cell viability, proliferation ability, lactose and β-casein secretion of DCMECs, which could also raise the milk yields of dairy cows significantly.


      PubDate: 2014-07-26T11:52:47Z
       
  • Comparison of Physiological Properties Between Dwarf and Vine-type
           Cucumbers (Cucumis sativus Linn.)
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Xin Ming , Qin Zhi-wei , Zhou Xui-yan , Wu Tao
      Dwarfism is one of the most important traits in crop breeding. In this study, a dwarf cucumber D0462 and a vine NA129 were taken as experimental materials. The metabolism of hydrogen peroxide (H2O2) and superoxide anion radical (O2 −•), activities of antioxidant enzymes, and the levels of endogenous hormones of the two cultivars were compared. In the whole growth period, D0462 maintained higher H2O2 and O2 −• levels than NA129. The activities of SOD (superoxide dismutase), CAT (catalase), APX (ascorbate peroxidase) and IAAO (indoleacetic acid oxidase) in D0462 were found higher than those in NA129. The contents of gibberellic acid (GA3), indole-3-acetic acid (IAA), zeatin riboside (ZR) and jasmonic acid (JA) in internode were determined by enzyme-linked immunosorbent assay (ELISA). The internode of D0462 remained lower levels of GA3, IAA, and ZR. In contrast, JA level in internode of D0462 was higher than that of NA129. Compared with NA129, the cell mumbers in internode of D0462 did not change, but cell size evidently decreased. The chloroplasts in mature mesophyll D0462 exhibited swollen filled with starch grains instead of regular oval ones, and thylakoids of chloroplast swelled and became ambiguous.


      PubDate: 2014-07-26T11:52:47Z
       
  • Identification of Powdery Mildew Pathogen and Ribosomal DNA-ITS Sequence
           Analysis on Melon
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Xue-zheng Wang , Bing-yin Xu , Wang Ping , Fei-shi Luan , Hong-yan Ma , Ying-yuan Ma
      Identification of powdery mildew pathogens on melon (Cucumis melo) is important for melon breeding and disease-resistant germplasm selection. In this study, a powdery mildew pathogen that infected melon plants in Heilongjiang Province, China, was investigated in terms of host identification, morphological characteristics and phylogenetic relationships. The morphological characteristics of the pathogen were observed at five phases in the life cycle: germinating conidia, primary germ tube, hyphae, conidiophores, and colonization. The conidia were elliptical, colorless, catenulate, and the average length was 29.07 µm and average width was 17.82 µm. One ascus and eight ascospores were produced. DNA was extracted from 0.01 g conidiophores from a strain of powdery mildew pathogen that infected melon. ITS ribosomal DNA region (524 bp) was amplified with the universal ITS1 and ITS4 primers. The nucleotide sequence showed 100% similarity with ITS sequences for three Podosphaera fusca strains obtained from the GenBank database. The identity of the pathogen was confirmed as Sphaerotheca fuliginea. International standard differential hosts were used to identify S. fuliginea strain as 2F race. These results supported the notion that Podosphaera fusca was a synonym of S. fuliginea.


      PubDate: 2014-07-26T11:52:47Z
       
  • Bioinformatic Analysis of Functional Characteristics of miR172 Family in
           Tomato
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Jie Li , Yu-shi Luan , Jun-miao Zhai , Ping Liu , Xiu-ying Xia
      MicroRNAs, a class of endogenous non-coding small RNAs about 21 nucleotides in length, play pivotal roles in plant physiological and biochemical processes. Tomato is important economic crop throughout the world. Recently, involvement of miRNAs in tomato has received significant attention. MiR172 is one of the conserved miRNAs in tomato. Investigation into the roles andthe target genes of this small RNA molecular in Arabidopsis illustrated that miR172 functions in regulating the transitions between developmental stages and in specifying floral organ identify. Herein, we combined bioinformatics and molecular biology approaches to profile the functional characteristics of miR172 in tomato. The results of target prediction showed that AP2-like ethylene-responsive transcription factors were major targets of miR172, analysis of upstream sequence indicated the presence of stress-related cis-elements in its promoter regions, and further analysis of qRT-PCR confirmed that miR172 participated in various stress responses. Our research provided a paradigm for further in-depth investigation of the functional characteristics of miRNAs in tomato.


      PubDate: 2014-07-26T11:52:47Z
       
  • Cloning and Expression Analysis of Sucrose Non-fermenting-1 Related
           
    • Abstract: Publication date: December 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 4
      Author(s): Hong-mei He , Zhi-wei Qin , Zhuo Feng , Tao Wu
      The sucrose non-fermenting-1 related protein kinase (SnRK), whose expression is induced by kinds of hyperosmotic stresses, plays a key role in improving stress resistance of plants. In order to investigate the molecular mechanism of low nitrogen resistance in cucumber, the full-length cDNA of SnRK gene was cloned in this study. The result showed that SnRK gene was 1 548 bp in length, encoded 515 amino acids, and had more than 80% homology with other crops. The protein encoded by this gene was an unstable and hydrophilic protein with no transmembrane structure and no signal peptide. Under nitrogen-free conditions and low nitrogen conditions, the expression pattern analysis of SnRK gene showed that this gene was up-regulated and its expression increased and was significantly higher than the normal level as the nitrogen concentration decreased. In addition, the expression of SnRK gene was also inhibited in the high nitrogen level and was significantly lower than the normal level. The result of this study would help us understand the molecular mechanism of low nitrogen resistance in cucumber.


      PubDate: 2014-07-26T11:52:47Z
       
  • Types of Maize Virus Diseases and Progress in Virus Identification
           Techniques in China
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Cui Yu , Zhang Ai-hong , Ren Ai-jun , Miao Hong-qin
      There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, maize crimson leaf disease, maize wallaby ear disease and corn lethal necrosis disease. This paper reviewed their occurrence and distribution as well as virus identification techniques in order to provide a basis for virus identification and diagnosis in corn production.


      PubDate: 2014-07-26T11:52:47Z
       
  • Biogenesis of Plant MicroRNAs
    • Abstract: Publication date: March 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 1
      Author(s): Kong Wen-wen , Wang Hong-bo , Li Jing
      microRNAs (miRNAs) play important regulatory roles in eukaryotic gene expression, predominantly at the posttranscriptional level. Elaborate and diverse biogenesis pathways have evolved to produce miRNAs. miRNA biogenesis is a multistep process including transcription, precursor slicing, methylation, nuclear export, and RNA-induced silencing complex assembly. In the decade, since the first discovery of plant miRNAs, many enzymes and regulatory proteins involved in miRNA biogenesis in plants have been uncovered and a basic picture of miRNA processing is emerging gradually. In this article, we summarized the current study of plant miRNA biogenesis and discussed the multiple integrated steps and diverse pathways of miRNA processing.


      PubDate: 2014-07-26T11:52:47Z
       
  • Regulation of Foliar Application DCPTA on Growth and Development of Maize
           Seedling Leaves in Heilongjiang Province
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Gu Wan-rong , Meng Yao , Zhang Jun-bao , Ji Biao , Wang Yong-chao , Li Jing , Wei Shi
      DCPTA (2-diethylaminoethyl-3, 4-dichlorophenylether) is a new plant regulator which can be used to regulate growth and development for crops. Experiments on maize seedlings were conducted in the growth chamber to study the effects of foliar applied DCPTA. The plant pots were placed in a completely randomized design with three replicates. The maize seedlings were treated with 0 mg • L−1 (control), 20 mg • L−1 and 40 mg • L−1 DCPTA solution. The effects of DCPTA on the photosynthetic characteristics (photosynthesis, stomata conductance, intercellular CO2, and transpiration rate), related physiological characteristics (contents of soluble sugar and starch), chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm, Fv/Fo, qP, and qN) and the weight of dry matter in maize seedling were studied. The results showed that DCPTA enhanced photosynthesis of maize seedling. In general, photosynthetic rate in leaves was significantly promoted through spraying DCPTA solution, and 40 mg • L−1 DCPTA was found to be the best concentration for maize. The relationship between stomata conductance and transpiration rate in maize leaves could be described as linear. With regard to the chlorophyll fluorescence parameters, our findings showed that 40 mg • L−1 DCPTA in maize seedling caused an increase in Fm, Fv/Fm, Fm/Fo, qP and a decrease in Fo and qN at some time points checked. It is suggested that DCPTA increased photosynthetic rate by raising both the content of chlorophyll and activities of PSII and the contents of sugar and starch. Compared with the control, the treated maize seedling caused an increase in plant height, root length, shoot dry mass, root dry mass, or the total (root plus shoot) dry mass.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effects of CuSO4 and Uniconazole on Mature Embryo Culture in Japonica Rice
    • Abstract: Publication date: June 2014
      Source:Journal of Northeast Agricultural University (English Edition), Volume 21, Issue 2
      Author(s): Shan He , Zou Deng-tang
      In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10–15 μmol • L−1 CuSO4 and 0.50–1.00 mg • L−1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol • L−1. When the concentration of CuSO4 was 15 μmol • L−1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol • L−1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg • L−1 and for Longjing 24 was 1.00 mg • L−1.


      PubDate: 2014-07-26T11:52:47Z
       
  • Effect of Different Stocking Rates on Deyeuxia angustifolia Bog Meadow in
           Sanjiang Plain of China
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Wang Ming-jun , Shi Yin-tao , Zhang Cheng-yu , Cui Guo-wen , Hu Guo-fu , Li Jing-xin , Yin Xiu-jie , Li Hai-xian
      This paper investigated the effects of cattle grazing on herbage production and its nutrient and soil characteristics over the grazing season. The grazing experiment was conducted on Deyeuxia angustifolia Bog meadow grassland established in 2010 in Sanjiang Plain. Four grazing treatments were light grazing (LG), moderate grazing (MG), heavy grazing (HG), and a non-grazed exclosure (CK) with corresponding stocking rates of 0.6, 1.0, 1.4, and 0 AU • hm−2 • month−1, selected as three replications. And then found out the optimum carrying capacity after analyzed the nutrition balance between livestock and grassland. Heavy grazing led to a decline in grassland productivity and soil nutrients. The SOM and the TN content in the soil layer of 0–10 cm were much higher than that in the soil layer of 20–30 cm. More attention should be paid to the nutrient changes in the soil layer of 20–30 cm. Currently, studies showed that the reliable carrying capacity in this type of grassland was 1.0 AU • hm−2 • month−1. Inclusion of rangeland health monitoring and optimum stocking rate into grassland management model could facilitate the sustainable development of the grassland.


      PubDate: 2014-07-26T11:52:47Z
       
  • Mutagenesis of Arachidonic Acid-producing Mortierella Isabellina and
           Analyses of Δ6-desaturase Role by qPCR
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Yao Di , Yu Chang-qing , Yang Jian , Li Li-na
      Arachidonic acid (AA or ARA), an essential ω−6 polyunsaturated fatty acid (PUFA), can be produced by Mortierella isabellina. Mutagenesis on Mortierella isabellina As3.3410 was induced to raise ARA production. The mutant strain of YZ-124 had the highest ARA of 4.72 g • L−1, which was 5.5 times higher than that of the original strain 3.3410. mRNA expression level of Δ6-desaturase was determined in five different kinds of ARA-producing Mortierella isabellina after cultured for 7 days, and in the mutant strain YZ-124 over a 3–8 day time-course. In addition, the desaturase activity and ARA content were measured at the selected time points. The lowest expression of Δ6-desaturase was observed in the original strain and the highest expression in the mutant strain YZ-124, which increased with increasing time in culture. Furthermore, a positive correlation was observed between the expression levels of Δ6-desaturase and ARA content. Based on this, Δ6-desaturase played a significant role in ARA synthesis pathway in Mortierella isabellina.


      PubDate: 2014-07-26T11:52:47Z
       
  • A Two-step Biotechnological Process for Improving Nutrition Value of
           Feather Meal by Bacillus licheniformis S6
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Guo Gang , Chu Jie , Wang Jun-gao , He Qiu-xia , Liu Ke-chun
      A two-step biotechnological process was developed using Bacillus licheniformis S6 to provide a simple and economical procedure which significantly improved feather meal nutrition value. Compared with IFM (initial feather meal) and CFM (commercial feather meal), SFEFM (feather meal gained by solid fermentation and enzymolysis with continuous agitation) had a significant improvement (P<0.05) in vitro digestibility, contents of oligopeptides and soluble protein released in digestive juice by pepsin-pancreatin digestion procedure, furthermore, some deficient essential amino acids in feather protein (histidine, methionine, lysine) were enhanced. Comapared with CFM, the oligopeptides released into digestive juice of ISFM (feather meal obtained by the biotechnological process described in the paper with intermittent shaking) was significantly enhanced (P<0.05), and its in vitro digestibility was statistically (P>0.05) equivalent to CFM. The summary of the finding to IFM treatment and possible means of further improvements were also listed.


      PubDate: 2014-07-26T11:52:47Z
       
  • Back Ground Segmentation of Cucumber Target Based on DSP
    • Abstract: Publication date: September 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 3
      Author(s): Fang Jun-long , Zhang Dong , Qiao Yi-bo
      In order to realize automatic and accurate grading of cucumber, the first thing is to make sure the high accuracy and integrity in cucumber shape segmentation. As the core processor of this dissertation, DSP TMS320DM6437 acquired and processed digital image, it solved the common shadowing problem associated with the natural light. Ultimately, the background subtraction was proposed. Compared with the result of above-mentioned image data processing, the error rate of classic background subtraction method was often high. The result of optimization showed that the improved background subtraction method worked well, and it could meet an accurate segmentation of the fruit in comparison with the original methods.


      PubDate: 2014-07-26T11:52:47Z
       
 
 
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