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Journal Cover Journal of Northeast Agricultural University
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     ISSN (Print) 1006-8104
     Published by Elsevier Homepage  [2563 journals]
  • Effects of Low-temperature and Herbicide on Membrane Stability,
           Antioxidant Capacity, and Product of Metabolism in Barley Seedlings
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Kong Zhi-you , Qin Peng , Liu Ye-ju , Chen Jia , Wang Shuo
      In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15°C and 12 h-light per day for 7 days, and then subjected to herbicide treatment, prometryn (with the concentrations of 0, 0.15%, 0.30%, and 0.45%) or isoproturon (with the concentrations of 0, 0.30%, 0.60%, and 0.90%), and the SOD activity, the CAT activity, the POD activity, the MDA content, proline content, soluble protein content, electrical conductivity, and the rate of O2 − were determined and analyzed. The results showed that the low-temperature was the most important, and the treatment-time of low-temperature was another significant influencing factor on the physiological and biochemical indices of barley seedlings. However, all of the physiological and biochemical indices determined were not affected by the kinds of herbicides and herbicide concentrations, and the SOD was stable and should play the more prominent role on extracting of free radicals according to the stepwise regression and correlation. The herbicide concentrations should be increased in the future research for truly reflecting the effects of the herbicide concentration on the physiological and biochemical indices of barley seedlings.


      PubDate: 2013-05-25T09:45:23Z
       
  • Physiological and Biochemical Changes of IrlVHA-c gene Transgenic Tobacco
           Seedlings and Self-crossed Progeny Under NaHCO3 Stress
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Li Shan-shan , Yang Tao , Bi Xiao-lu , Fu Guo-hua , Wang Jin-gang
      In order to study the alkali resistivity of VHA-c in Iris lacteal. (IrlVHA-c), the transgenic tobacco seedlings harboring IrlVHA-c gene from Iris lactea (T0), the self-crossed progeny (T1), and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol • L−1 NaHCO3. The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol • L−1 NaHCO3, and activity was slightly lower at 300 mmol • L−1 NaHCO3. When the concentration of NaHCO3 was as high as 400 mmol • L−1 the seedlings were badly hurt. In addition, the activity of T0 and T1 transgentic tobacoo was maintained more or less. While the non-transgenic lines of tobacco could maintain viably up to 100 mmol • L−1 NaHCO3, and they could not survive at 400 mmol • L−1 NaHCO3. The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.


      PubDate: 2013-05-25T09:45:23Z
       
  • Ecuador Export Potential Agricultural Products to China
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Alvarez Alex , Wang Du-chun , Wang Pan-pan
      This paper presented a conceptual framework to country competitiveness using the Index of Competitiveness (IVCR), revealed for Ecuador to determine the most competitive and potential products exported to China; also measured the export complementarities through other indicators and by comparison with similar products exported by competing countries. This study used the 6-digit Harmonized System (HS) and the 10-digit NANDINA tariff codes for more specific items. Thereby we can identify trends that allow the Ecuadorian authorities analyze the state of bilateral agreements and develop action strategies, moreover help exporters identify potential market niches, considering that China is currently one of the developing countries with higher growth and expanding economy. The results showed that the most competitive agricultural products consisted on fish flour, bovine skins, mollusks, fish wastes and cocoa grains. The founded potential products were broad beans, fruits, fish viscera, algae, alpaca hair or hair wastes. Nevertheless these were none adding value commodities we need to find some mechanisms to deepen the exportable offer of value-added goods.


      PubDate: 2013-05-25T09:45:23Z
       
  • Research on DSP-Based Automatic Excitation Regulator in Small Rural
           Hydropower Station
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Gu Xiang-yu , Zhao Yu-lin , Fu Yan , Zhang Yu-juan , Sun Shuai
      Excitation regulator has played an important role to ensure that generator run safely and stably. However, in some remote areas, the rural small generator excitation control system is very backward, and it has hindered the development of Chinese agriculture and the pace of new countryside construction. This paper introduced a kind of structure and basic principle of DSP-based automatic excitation regulator, which was applied to rural small generator. We chose TMS320LF2407A chip as the core of regulator control system; the regulator adjusted PWM based on PID control strategy to control the exciting current. The theoretical analysis showed that the regulator had the characteristics of simplicity and reliability, well dynamic and static characteristics, and it could also adjust its own state quickly. The result was applicable to small rural hydropower station.


      PubDate: 2013-05-25T09:45:23Z
       
  • Establishment and Analysis on Material Flow Model in Argo-animal Husbandry
           Ecosystem
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Yin Hai-dong , Liu Zhen-zhong , Wu Qiu-feng
      This paper analyzed the material flow situation in argo-animal husbandry ecosystem by compartment model. This model was an important mean for investigating the whole structural characteristics in ecosystem. Based on this analysis, characteristics of material cycle and integrity in the system were mastered. As an example of natural conditions in Yonghe Village, Shuangcheng Township, Shuangcheng Municipal, Heilongjang Province, the system of linear differential equations in system was established by extracting each compartment and investigating material flow and stability of this model was proved by Lyapunov linear theory. The result showed that this system could not be interfered by initial value in the state of present, input and output.


      PubDate: 2013-05-25T09:45:23Z
       
  • Drying Characteristics of Microwave-assisted Foam Drying of Corn Soaking
           Water
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Li Qiang , Sun Yu , Xu Xiang-wen , Zhang Qin-qin , Zheng Xian-zhe
      To improve the industrial utilization of corn soaking water, the yeast protein powder was produced by microwave-assisted foam drying. Firstly, preparation experiments were carried out, which included the foaming characteristic experiments of fermentation broth and that about the effects of carbon and nitrogen ratio on solid content in the fermentation broth. Secondly, the drying characteristics experiment of corn soaking water was studied, which selected the microwave power, material amount and thickness as the influencing factors for the single experiment. The results showed that the final moisture content (d.b.) and drying rate were significantly affected by those factors.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Sub-chronic Aluminum Exposure on Renal Pathologic Structure in
           Rats
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Xia Shi-liang , Li Miao , Shao Bing , Bai Chong-sheng , Zhang Ji-hong , Li Yan-fei
      A total of 40 Wistar rats, weighing 130-140 g, were allocated randomly into four groups. They were orally administrated with 0 (control group, GC), 64.18 (low-dose group, GL), 128.36 (middle-dose group, GM), and 256.72 (high-dose group, GH) mg aluminum chloride (AlCl3) per kilogram body weight in drinking water for 120 days. Kidney coefficient and aluminum (Al) concentrations in blood and kidney were determined, and renal autopsy and histological changes were observed. The results showed that kidney coefficient in all Al-treated groups were obviously lower than that in GC (P<0.01) and there was a dose-effect relationship. The kidneys were solid, lusterless and pale brown with white necrosis point on surface. Under electron microscope, renal cortex became thin, the renal tubule was narrowed and the epithelium dissolved; the renal glomerulus became atrophied and the glomerular became vasodilator. The Al concentrations in blood and kidney were higher in all Al-treated rats than those in GC (P<0.01), and there was a dose-effect relationship. The results indicated that sub-chronic Al exposure could lead to Al accumulation in kidney, restrain the development of kidney and cause the pathologic damage in rats.


      PubDate: 2013-05-25T09:45:23Z
       
  • Expression of a Lysine-rich Gene in Bacillus subtilis 168
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Li Xue-lin , Liu Xiao-fei , Gao Xue-jun , Qiao Bin , Pan Hong-bao , Ao Jin-xia
      Lysine-rich protein gene (lys) was cloned from winged bean (Psophocarpus tetragonolobus (L.) DC), and cloned into prokaryotic expression vector pHT43, the recombinant plasmid pHT43/lys were constructed and then transferred into Bacillus subtilis168, upon IPTG induction, the recombinant protein was expressed, and the content of lysine was detected by HPLC. The result showed that lysine content increased by 9.85%. It was suggested that introducing lys gene into Bacillus subtilis 168 was an effective way to improve its nutrition quality.


      PubDate: 2013-05-25T09:45:23Z
       
  • Isolation, Purification and Cryopreservation of Cells from Neonatal Bovine
           Testis
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Zheng Peng , Hu Peng-fei , Tian Ya-guang , Huang He , Zhang Gui-xue
      The development and application of spermatogonial stem cell technology have an important significance in animal cloning, preservation of endangered species and spermatogenesis research. In this study, the seminiferous epithlium cells were isolated and purified, the cells were cryopreserved after identification, and the effects of different purification and cyopreservation methods on bovine testicular cells were studied. The results showed that there were spermatogonial stem cells and sertoli cells in the neonatal bovine seminiferous tubules, differential adherent selection methods could effectively separate these two cell types. Spermatogonial stem cells were positive after AKP, C-kit, and OCT-4 identification; sertoli cells were positive after oil red O and vimentin identification. Frozen stock solution supplemented with 10% DMSO had the best effect in spermatogonial stem cell cryopreservation, while frozen stock solution supplemented with 10% of ethylene glycol and 0.1 mmol • L−1 trehalose had the best effect in sertoli cells cryopreservation.


      PubDate: 2013-05-25T09:45:23Z
       
  • Molecular Typing of Aeromonas hydrophila Isolated from Common Carp in
           Northeast China
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Li Shao-wu , Wang Di , Liu Hong-bai , Lu Tong-yan
      A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus (ERIC-PCR) method. The results showed that three different types, i.e. type I, II, and type III, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed III> I > II, while the isolates of Liaoning Province showed III> II> I. The percentage of different types in different provinces varied in each other; however, they didn't show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.


      PubDate: 2013-05-25T09:45:23Z
       
  • Genetic Dissimilarity of Clonal Populations of Leymus chinensis (Trin.)
           Tzvel in Heterogeneous and Homogeneous Habitats in Southwestern
           Heilongjiang Province of China
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Hong Rui-min
      Leymus chinensis (Trin.) Tzvel is the characteristic of the Eastern Asia, and is a perennial herbaceous rhizome species on L. chinensis steppe of the Northern China, the Eastern Mongolia, and area of Outer-Baikal of Russia. It has both vegetative and sexual reproductions, and results in complexity of the population structure. Thus, it is difficult to identify distinct clones. Author used the randomly amplified polymorphic DNA (RAPD) technique to identify the clonal structure of heterogeneous and homogeneous habitats in two populations of Leymus chinensis, analyzed 260 ramets samples using 12 primers and 60 polymorphic markers (reproducible RAPD bands). The results proved clonal diversity described using PD, D, and E was higher in the HCS habitat than that in the HPS habitat. The PD, D, and E in the HCS of Songzhan population were 0.21, 0.90, and 0.91, respectively, whereas the PD, D, and E in the HPS of Songzhan population were only 0.09, 0.68, and 0.77, respectively; the PD, D, and E in the HCS of Yongle population were 0.19, 0.92 mad 0.90, respectively, correspondingly, these measures in the HPS of Yongle population were only 0.09, 0.63, and 0.79, respectively. The results also indicated that clonal population of L. chinensis in HPS habitat had lower genetic dissimilarity index than that in the HCS habitat.


      PubDate: 2013-05-25T09:45:23Z
       
  • ISSR Analysis of Hybrid Descendants of Roses
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Che Dai-di , Su Cheng-yuan , Zhang Jin-zhu , Chen Xue , Ma Xue , Wang Na , Li Wen-tian
      Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F1 offspring in this research.


      PubDate: 2013-05-25T09:45:23Z
       
  • SWOT Analysis and Development Strategies of Maize Industry in Heilongjiang
           Province
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Gu Wan-rong , Jin Yi , Meng Yao , Wang Jian-guo , Zhao Xian-long , Li Jing , Wei Shi
      With the development of China's market economy, the international grain supply situation is increasingly tense, and the grain has been regarded as an important international strategic resource in the international market. Due to the reduction of international grain output and expected inflation, the gap between maize supply shortage and increasing demand is apparent in China, so that China is described as a worrying importer country. By using SWOT analysis belonging to the field of enterprise strategic management, this paper analyzed the current maize industry development in Heilongjiang Province as an entry point, the strength, weakness and opportunity and threat were put forward. Thereby, the developing principles and strategies were proposed to stimulate the maize industry development in Heilongjiang Province, which would lay a good foundation of 50 billion kilograms grain production capacity by 2015 in China, as well as the economic development and local farmers' income increase.


      PubDate: 2013-05-25T09:45:23Z
       
  • Research Progress on Water Use Efficiency and Drought Resistance of
           Turfgrass
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Li Xin , Yan Qing-wei , Zhang Lu , Liu Hui-min , Zheng Hai-xia , Bao Wen-long , Chen Ya-jun
      Improving turfgrass drought resistance and save-water features are very important to solve water deficiency in turfgrass production and management. This research did some surveys of systematic literature review about turfgrass drought tolerance morphological characteristics, physiological characteristics and water use efficiency, and provided the detailed information for enhancing turfgrass drought resistant and water use efficiency in the future.


      PubDate: 2013-05-25T09:45:23Z
       
  • Advanced Progress on Adaptive Stress Response of Oenococcus oeni
    • Abstract: Publication date: March 2013
      Source:Journal of Northeast Agricultural University (English Edition), Volume 20, Issue 1
      Author(s): Zhao Wen-ying , Kang Zhen-kui
      Oenoccoccus oeni is an alcohol-tolerant, acidophilic lactic acid bacterium with its ability to perform malolactic fermentation in wine, which is of fundamental importance in oenology. As a representative of the wine bacterium with remarkable adaptability to the very harsh physicochemical conditions of wine, many studies were carried out for its applied interest and focused mainly on its stress response mechanisms of O. oeni. on both physiological and molecular levels. In this review, three main stress response mechanisms in O. oeni during culturing process were addressed. Of them, various solute transporters and secondary metabolic energy-generating systems were utilized to control the intracellular environment and the energetic status of O. oeni. The changes in cell membrane fatty acid composition profiles and synthesis of stress proteins, especially small heat shock proteins were required for active cell response to maintain membrane integrity and function under stress conditions. The study on stress response of O. oeni played an important role on culture bacteria selection, making inoculation culture and construction of other engineering bacteria.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Phosphate Fertilizer on Cold Tolerance and Its Related
           Physiological Parameters in Rice Under Low Temperature Stress
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Hou Li-gang , Chen Wen-fu , Zhao Guo-chen , Ma Wei , Qi Chun-yan , Liu Liang , Sun Hong-jiao
      The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings (chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81) under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Row-Spacing on Canopy Structure and Yield in Different Plant
           Type Rice Cultivars
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Zhao Hai-xin , Wang Xiao-xue , Guo Zhen-hua , Huang Xiao-qun , Liu Hua-long
      Two japonica rice varieties, Longjing 20 (more tillers and curved panicle type, MCP) and Longjing 21 (few tillers and half erect panicle type, FEP), were used to study the effects of row-spacing on canopy structure, morphological characteristics and yield. The results showed that the percentage of productive tiller reduced first, and increased afterwards as row-spacing increasing. The relationship between row spacing and the percentage of productive tiller fitted a quadratic regression. The effects of row spacing on leaf area index (LAI) at later tillering stage and the highest stem number per square meter also followed a quadratic regression relationship with increasing first and then reducing. The effects of row-spacing on primary branch were larger than the secondary branch in Longjing 20. However, the trend in Longjing 21 was opposite. The relationship between row spacing and seed setting rate of the secondary branch or panicle was negatively correlated. An extreme significant negative correlation was obtained between seed setting rate of secondary branch in Longjing 20. There was no significant positive correlation between row-spacing and yield in Longjing 20 (R2=0.68). However, the negative correlation between row-spacing and yield of Longjing 21 was extremely significant (R 2=−0.96**). The canopy structure of MCP was more sensitive to row-spacing. The positive correlation between row spacing and the length of the flag leaf (R2=0.89**), the width of the flag leaf (R2=0.85*), the length of the last internode (R2=0.85*), the length of the last 2nd internode (R2=0.96**) or the length of the panicle (R2=0.91**) was significant or extremely significant in Longjing 20, but not in Longjing 21. The wider row-spacing promoted the accumulation of the dry matter of panicle, stem and leaf and the yield formation in MCP. The best row-spacing in Longjing 20 was 30 cm. For Longjing 21, the narrower row-spacing was better. The best row-spacing of it was 21 cm. These results suggested that improved the population environment of MCP or the utilization of the free space in the field of FEP could be reached either by wider row-spacing or narrow row-spacing.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Irrigation Schedules on Photosynthetic Carbon Assimilation of
           Winter Wheat (Triticum aestivum L.) in North China Plain: from Leaf to
           Population
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Liu Li-Ping , Ouyang Zhu
      A field experiment was conducted to elucidate the regulation mechanism of different irrigation schedules on population photosynthetic of winter wheat. The experiment included five irrigation schedules, such as no irrigation (W0), irrigation once at jointing (W1j) or at booting (W1b), irrigation twice at jointing and booting (W2), and irrigation three times at jointing, booting and grain-filling (W3) and three planting densities, such as 180 (D1), 300 (D2) and 450 (D3) seedlings per square meter. The results indicated that irrigation significantly improved population photosynthesis. The relationship between population photosynthesis and irrigation time/volume was to some extent parabolic. Improvements in population photosynthesis (resulting from more irrigation time/volume) were mainly related to increase in leaf area index and population light interception. Population photosynthesis exhibited a significantly negative correlation with canopy light transmittance. Population photosynthesis at grain filling stage was significantly positively correlated with dry matter accumulation at post-anthesis and grain yield. Main effects and partial correlation analysis showed that population photosynthesis of W0, W1j, W1b and W3 were regulated by canopy light transmittance and leaf area. On the other hand, population photosynthesis of W2 was mainly influenced by flag leaf photosynthetic rate. On this basis, planting 300 seedlings per square meter was the optimum combination. The combination of W2D2 increased population photosynthesis during mid-late growth stages and extended high population photosynthesis duration, which ultimately increased grain yield.


      PubDate: 2013-05-25T09:45:23Z
       
  • Development of Molecular Marker Linked to Cf-10 Gene Using SSR and AFLP
           Method in Tomato
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Li Ning , Jiang Jing-bin , Li Jing-fu , Xu Xiang-yang
      The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.


      PubDate: 2013-05-25T09:45:23Z
       
  • Study on Classification and Genetic Diversity of Kentucky Bluegrasses by
           Using RAPD Markers
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Wang Chao , Li Xin , Gao Li-na , Zhang Lu , Liu Wei , Liu Hui-min , Chen Ya-jun
      A total of 69 random primers were screened by using random amplified polymorphic DNA (RAPD) markers to analyze the genetic bands of 32 Kentucky bluegrass cultivars. A total of 197 bands were amplified from 46 primers, among which 195 bands were polymorphic. Each primer could amplify one to nine polymorphic bands with an average of 4.3 per primer. Based on similarity coefficient analysis of RAPD results and by using NTSYS software to cluster analyze with the average UPGMA method, the result showed that 18 cultivars of the 32 were in group 1, three cultivars were in group 2, two cultivars were in group 3, eight cultivars were in group 4, and only one cultivar in group 5.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Different Cropping Patterns of Soybean and Maize Seedlings on
           Soil Enzyme Activities and MBC and MBN
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Yan Mu-chun , Xu Ting-ting , Song Peng-hui , Dai Jian-jun
      Through the pot experiment, the effects of different cropping patterns of soybean and maize seedlings on rhizosphere soil urease, catalase, polyphenol oxidase and invertase activities and microbial biomass carbon (MBC) and nitrogen (MBN) were studied. Six treatments of soybean-soybean, soybean-maize, soybean-mixed, maize-soybean, maize-maize and maize-mixed were conducted in pots. Results showed that catalase activity and invertase activity of maize-maize were the highest and significantly different from those of the other treatments except maize-soybean; soil polyphenol oxidase activity of soybean-maize was the highest, and reached significant level among the other treatments, but there was no significant difference of urease activity among treatment soils; MBC of maize-maize soil and MBN of maize-mixed soil reached the highest and significant levels compared with other treatments; MBC and C/N ratio had positive and very significant correlations with soil catalase activity and invertase activity, respectively. Therefore, different cropping patterns could affect rhizosphere soil enzyme activities and soil MBC and MBN, which influenced soil carbon and nitrogen mineralization.


      PubDate: 2013-05-25T09:45:23Z
       
  • Establishment of TaqMan Real-time Quantitative PCR Assay for Foreign Gene
           Copy Numbers in Transgenic Soybean
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Qiu You-wen , Gao Xue-jun , Qi Bang-ruo , Li Lu , Zhen Zhen
      TaqMan quantitative PCR technique was used to detect the copies of exogenous CaMV35S flanks sequence in transgenic soybean. With soybean lectin as the endogenous reference gene, and gene complex DNA in non-GMO soybeans as the endogenous reference standard, the gradient dilution method was used to separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies, and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation. The standard curve equation of endogenous reference gene was y=−3.422x+35.201, R2=0.998; the standard curve equation of exogenous gene was y =−3.495x+35.303, R2=0.999. The sample copies was got by putting Ct value into the standard curve equation, and it was the ratio of exogenous gene and reference gene. We found that CaMV35S gene in transgenic soy was single copy.


      PubDate: 2013-05-25T09:45:23Z
       
  • Study on Semen Freezing Preservation of German Shepherd Dogs
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Huang He , Tian Ya-guang , Zheng Peng , Zhang Gui-xue
      In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 (P<0.01). The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method (P<0.01). The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Subchronic Aluminum Exposure on Amino Acids Neurotransmitters
           in Chicken Brain
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Han Yan-fei , Xia Shi-liang , Bai Chong-sheng , Zhang Ji-hong , Li Yan-fei
      To investigate the effects of aluminium (Al) exposure on amino acid neurotransmitters, the chickens with different levels of subchronic Al poisoning were estabolished by continuous peritoneal injection of fixed volume and different concentrations of gradient of aluminium trichloride (AlCl3). The levels of amino acid neurotransmitters in chicken brains were determined by high performance liquid chromatography (HPLC) after being exposed of Al for 60 days, and Al levels in serum and brain tissue were determined by atomic absorption spectrophotometry (AAS). The results showed that Glu levels increased with the increase of Al, but there was no significant difference compared with the control. The levels of Al, Asp, Gly, GABA and Tau were significantly higher in Al-treated groups than those in the control. The results indicated that Al intoxication led to excitatory neurotoxicity.


      PubDate: 2013-05-25T09:45:23Z
       
  • In Vitro Study on Commercial Organic Acid Activate WD (WD) Against Four
           Pathogenic Bacteria
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Jiang Guo-zheng , Li Ji-chang , Han Zhen-xing , Liu Ting , Wang Yuan , Cao Hong
      To investigate the antibacterial activity of commercial organic acid Activate WD (WD), the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of WD against Staphylococcus aureus, Escherichia coli, Salmonella pullorum, and Campylobacter jejuni were determined by double broth dilution method. Bacteria were added in a mixture of water and commercial broiler feed adjusted by WD into pH 3.0, 4.0, and 5.0; the survival of bacteria was determined by a direct plate count method at 0, 0.5, 1, 4, 8, and 24 h after being mixed to test the bactericidal potential of WD. The results showed that the MIC of WD against four bacteria was 0.3125%, and the MBC was 0.625%. At pH 3.0, bacteria could not survive. Campylobacter jejuni died after 4 h of incubation at pH 4.0, 5.0, whereas for other three bacteria, the bacterium numbers were below detection limits until 8 h of incubation. In conclusion, WD had significant antibacterial activity, and could be used on farms to prevent cross-infection via rearing water.


      PubDate: 2013-05-25T09:45:23Z
       
  • Response of Osmotic Adjustment of Lactobacillus bulgaricus to NaCl Stress
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Li Chun , Liu Li-bo , Sun Di , Chen Jing , Liu Ning
      Growth and osmotic response of Lactobacillus bulgaricus ATCC 11842 under hyperosmotic constraint were investigated in a chemically defined medium (CDM) and MRS medium. NaCl could inhibit the growth of L. bulgaricus which decreased with increasing NaCl concentration. In the MRS, NaCl of 1.0 mol · L−1 was the biggest salt stress concentration; in the CDM, 0.8 mol · L−1 was the biggest inhibition concentration. In contrast to what was observed in other lactic acid bacteria, proline, glycine betaine and related molecules were unable to relieve inhibition of growth of L. bulgaricus under osmotic constraint. This was correlated to the absence of sequences homologous to the genes coding for glycine-betaine and/or proline transporters described in Lactococcus lactis and Bacillus subtilis. The amino acid aspartate and alanine were proved to be osmoprotective under NaCl stress. Addition of peptone (0.25% w/v) in the presence of salt led to a stimulation of the growth, as the decrease of the lag time and generation time, and the final biomass increased from 0.31 to 0.64.


      PubDate: 2013-05-25T09:45:23Z
       
  • Temperature Simulation of Berry Slices Under Microwave Vacuum Puffing
           Conditions
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Liu Hai-jun , Lin Zhen , Zheng Xian-zhe , Liu Cheng-hai , Song Xiang-yu , Liu Dai-ya
      In order to study the temperature simulation of berry slices in microwave vacuum puffing process, numerical simulation method was used to simulate the temperature in different time according to theoretical calculation result and the deviation had been verificated throughout the experiment. The better uniform of temperature between experiment and simulation could be concluded except in the period of rapid temperature increase. The temperature reached the high point of 130.25°C in the middle point of slices at 100 s, while the temperature was over 50°C in external parts of slices for the reason of thermal diffusion. Conclusions could be drawn that the model was effective and numerical analysis could simulate the temperature simulation for berry leather in microwave vacuum puffing process.


      PubDate: 2013-05-25T09:45:23Z
       
  • Storage System Design Scheme in Virtualization Construction
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Si Zhen-yu
      In order to improve resource utilization, it is necessary to integrate storage and data, and the emergence of cloud computing makes it possible. This paper analyzed the study of virtualization and cloud computing, proposed a new scheme based on virtualization, and established a shared storage platform, which made a good complement and perfected the centralized storage platform.


      PubDate: 2013-05-25T09:45:23Z
       
  • Study on Improving Development Strategies of New Rural Social Pension
           Insurance System in Heilongjiang Province
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Zhang Qi-wen , Li Hui-fang , Gao Li-na
      Since 2009, the new rural pension insurance in Heilongjiang Province has obtained certain achievements, but the effects are not obvious. Reflection on the development process, we find that there are a lot of problems. Therefore, in order to guarantee the smooth implementation of new rural social pension insurance work in Heilongjiang Province, this paper combined with the reality to provide several suggestions for improving the new rural social pension insurance system in Heilongjiang Province.


      PubDate: 2013-05-25T09:45:23Z
       
  • Approaches to Promote China's Low Carbon Agricultural Development
    • Abstract: Publication date: December 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 4
      Author(s): Chen Yao
      Low carbon agriculture is a new production mode of green agriculture development to face the challenge of the global climate change, and a kind of innovation on developing circular agriculture. Up to now, low carbon agricultural development approaches are lacking of deep and systematical researches. Therefore, the paper briefly analyzed the situation and the existing problems of China's low carbon agricultural development, and then put forward the approaches to promote the low carbon agricultural development.


      PubDate: 2013-05-25T09:45:23Z
       
  • Regulatory Network of Transcription Factors in Response to Drought in
           Arabidopsis and Crops
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Li-miao Chen , Li Wen-bin , Zhou Xin-an
      Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in Arabidopsis, rice and other crops. To better understand reaction mechanism of plant to drought tolerance, we mainly focused on introducing the research of transcription factors (TFs) in signal transduction and regulatory network of gene expression conferring drought. A TF could bind multiple target genes to increase one or more kinds of stress tolerance. Sometimes, several TFs might act together with a target gene. So drought-tolerance genes or TFs might respond to high-salinity, cold or other stresses. The crosstalk of multiple stresses signal pathways is a crucial aspect of understanding stress signaling.


      PubDate: 2013-05-25T09:45:23Z
       
  • Comparison of Net Photosynthetic Rate in Leaves of Soybean with Different
           Yield Levels
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Li Da-yong , Zhang Zhi-an , Zheng Dian-jun , Jiang Li-yan , Wang Yuan-li
      A total of nine soybean (Glycine max (L.) Merr.) cultivars were divided into three yield levels which were planted under the same environmental condition. The net photosynthetic rate was measured by LI-6400 portable photosynthesis system. The chlorophyll content and specific leaf weight were measured with regular methods. The results showed that the specific leaf weight, chlorophyll content and net photosynthetic rate of high yield varieties were higher than those of low yield varieties. The yield had a significantly positive correlation with the net photosynthetic rate. With the improvement of modern technology, the net photosynthetic rate could be measured quickly and exactly. Hence, net photosynthetic rate could be used as an effective index in the selection of high yield soybean.


      PubDate: 2013-05-25T09:45:23Z
       
  • Multiplex PCR System Optimization with Potato SSR Markers
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Wang Shao-peng , Liu Shang-wu , Li Yong , Liu Wei-ting , Lv Dian-qiu
      Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components (enzyme, MgCl2, DNA template and dNTPs) in PCR system were used in the research with the concentration of the other component remained the same; the orthogonal design L9 (34) was applied in the optimization of four sets of primers (STM0014, Pat, SSI, and UGP) in the reaction system at three levels; the temperature gradient selection was used to find out the optimum annealing temperature for the primer. The optimized multiplex PCR system of potato SSR marker with a total volume of 20 μL: 2.5 μL 25 mmol • L−1 MgCl2, 0.6 μL 10 mmol • L−1 dNTPs, 0.8 U Taq, 80 ng DNA template was ultimately established through the comparison and analysis of test results; the ratio of four pairs of 4 mmol • L−1 primers was 2: 1: 2: 3, and the annealing temperature was 54.7°C. The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties. This research built the solid foundation for the further study of genetic diversity of potato germplasms and construction of DNA fingerprinting.


      PubDate: 2013-05-25T09:45:23Z
       
  • Analysis on Combining Ability for Characters of Male Sterile Lines in
           Rapeseed (Brassica napus L.)
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Huang Ze-su , Laosuwan Paisan , Machikowa Thitiporn , Chen Ze-hui , Dai Wen-dong , Tang Rong , Li Dezhen
      The male sterile line is very important in the hybrid breeding program of rapeseed. This study was conducted to evaluate the combining ability of many characters of male sterile lines in Brassica napus L. Ten recessive genetic male sterile (RGMS) lines were used as parents to produce 45 single cross hybrids by using a half diallel cross method. These 45 crosses and their 10 parents were evaluated at Guiyang during 2007–2008. The results showed that both general combining ability (GCA) and specific combiing ability (SCA) effects were important for all characters, but additive gene effects were more predominant than non-additive gene effects. Qianyou 8AB and You 2894AB gave respective highly significant GCA effects of 230.94 and 127.65 kg • hm−2 for seed yield. Lines You 2894AB, QH303-4AB, You 157AB and You 2341AB gave highly significant GCA effects for oil content of 0.99, 1.62, 1.20 and 1.53%, respectively. The crosses among lines Qianyou 3A×Qianyou 8B, Qianyou 8A×You 2894B, You 2894A×Qianyou 6B, Qianyou 8A×QH303-4B and Qianyou 8A× Qianyou 6B gave high SCA effects of 616.29, 398.71, 356.48, 394.24 and 303.79 kg hm−2 for seed yield, respectively. All these crosses also gave high seed yield indicating that these crosses could be used in the breeding program.


      PubDate: 2013-05-25T09:45:23Z
       
  • Study on Mutant Induction of Gladiolus by in vitro Culture of Petals
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Li Cai-hua , Fan Jin-ping , Gong Shu-fang , Che Dai-di
      Petals of Gladiolus ‘Rose Supreme’ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in M1 generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS+2, 4-D 4.0 mg • L−1+6-BA 0.5 mg • L−1 and the best medium to induce somatic embryogenesis was MS+2, 4-D1.0 mg • L−1+TDZ0.3 mg • L. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.


      PubDate: 2013-05-25T09:45:23Z
       
  • Research on Tobacco Transformation of Vacuolar H+-ATPase Subunit c Gene
           from Iris lacteal
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Wu Duo , Zhou Ai-min , Shi Sheng-feng , Li Shan-shan , Wang Jin-gang
      Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaCl for 7 days. Detected the MDA content, the SOD activity and the POD activity. Moreover, the transgenic plants displayed obviously better growth than the normal plants under salt stress. These results suggested that VHA-c could confer the stress tolerance through enhancing the POD and the SOD activities, and by protecting membranes from damage by decreasing lipid peroxidation under salt stress.


      PubDate: 2013-05-25T09:45:23Z
       
  • Genome-wide Analysis of Ovate Family Proteins in Arabidopsis
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Huang Jian-ping , Li Hong-ling , Chang Ying
      Arabidopsis thaliana ovate family proteins (AtOFPs) is a newly found plant-specific protein family interacting with TALE (3-aa loop extension homeodomain proteins) homeodomain proteins in Arabidopsis. Here, based on bioinformatic analysis, we found that Arabidopsis genome actually encoded 17 OVATE domain-containing proteins. One of them, AtOFP19, has not been previously identified. Based on their amino acid sequence similarity, AtOFPs proteins can be divided into two groups. Most of the AtOFPs were located in nuclear, four of them were presented in chloroplast and the remaining two members appeared in cytoplasmic. A genome-wide microarray based gene expression analysis involving 47 stages of vegetative and reproductive development revealed that AtOFPs have diverse expression patterns. Investigation of proteins interaction showed that nine AtOFPs only interacted with TALE homeodomain proteins, which are fundamental regulators of plant meristem function and leaf development. Our work could provide important leads toward functional genomics studies of ovate family proteins, which may be involved in a previously unrecognized control mechanism in plant development.


      PubDate: 2013-05-25T09:45:23Z
       
  • Regulation of Floral Organ Identity in Arabidopsis by Ectopic Expression
           of OsMADS58
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Wang Yan-mei , Yan Da-wei , Zhang Ying-ying , Li Jing , Cang Jing
      The ABCDE model has been described comprehensively to interpret the mechanism of floral organ development. In Arabidopsis, AGMAOUS was a well-studied class C gene encoding a transcription factor with MADS box, and its overexpression in Arabidopsis induced the transformation of sepals to carpels and petals to stamens. However, there is little knowledge about the function of OsMADS58, which was a homologue of AGMAOUS in rice. In our study, OsMADS58 driven by the UBIQUITIN promoter was ectopicly expressed in Arabidopsis., and carpel-like sepals, incomplete petals and stamens instead of petals were found in different transgenic plants according to different OsMADS58 mRNA expression levels. The phenotype of transgenic plant was similar to the AGMAOUS overexpression plant, suggesting that OsMADS58 might play an important role in flower development like AGMAOUS and they keep conserved function during evolution.


      PubDate: 2013-05-25T09:45:23Z
       
  • Impact of let-7g on Proliferation and Lactation of Mouse Mammary
           Epithelial Cells
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Feng Li , Li Qing-zhang , Cui Wei , Ding Wei
      Let-7g, a member of the let-7 family, regulates gene expression at the post-transcriptional level. The study explored a series of biological effects of mouse mammary epithelial cells that let-7g was produced. The differential expression of let-7g was detected by qRT-PCR in different developmental stages of the mouse mammary gland. let-7g expression and impact of let-7g on mouse mammary epithelial cells were analyzed by CASY-technology, qRT-PCR, Western blotting and HPLC inhibited let-7g expression of mouse mammary epithelial cells through gene silencing. The results showed that qRT-PCR identified let-7g as being down-regulated in mouse mammary epithelial cells after it was inhibited. Mouse mammary epithelial cells with low expression of let-7g displayed higher expression of TGFβR I protein than those with high expression of let-7g, suggesting that low let-7g expression contributed to TGFβR I over-expression. Finally, the expression of let-7g was down-regulated, which significantly enhanced the proliferation of mouse mammary epithelial cells, and increased expression of β-Casein. The data indicated that let-7g could negatively regulate the expression of target Tgfbr1 by complementary combination in mouse mammary epithelial cells, and then regulate the cell proliferation and expression of β-Casein by suppressing the TGFβR I expression.


      PubDate: 2013-05-25T09:45:23Z
       
  • Screen of Bovine Mammary Gland Epithelial Cell Specifcity Promotor
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Liu Xiao-fei , Li Qing-zhang , Qiu You-wen , Gao Xue-jun
      Three lactoproteins (α-S1-casein, β-lactoglobulin, and β-casein) promotors were cloned, sequenced and compared relative luciferase expression. The results showed that the promotor activity of bovine α-S1-casein gene was the best, and would be used to produce pharmaceutically and medically important proteins in the mammary gland of transgenic animals and also for the construction of an inducible eukaryotic expression vector.


      PubDate: 2013-05-25T09:45:23Z
       
  • HPLC Analysis of α-lactalbumin and β-lactoglobulin in Bovine
           Milk with C4 and C18 Column
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Kong Xiao-yu , Wang Jing , Tang Yan-jun , Li Dan-dan , Zhang Nan-nan , Jiang Jin-dou , Liu Ning
      Reversed-phase high-performance liquid chromatography (RP-HPLC) method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C4 column (250 mm×4.6 mm×5 μm) and Shiseido CAPCELL PAK SG 300 C18 column (250 mm×4.6 mm×5 μm) were used in the experiment. Phase A was composed of 0.1% (V/V) trifluoroacetic acid (TFA) in ultrapure water and Phase B (organic phase) was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min−1. The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30°C. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%–104.38%, C18 column had stronger ability to resist acid and more stable, and the method with C4 column had excellent sensitivities and good separation.


      PubDate: 2013-05-25T09:45:23Z
       
  • Effects of Aluminum Trichloride on Structure and Function of Kidney in
           Chickens
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Zhang Ji-hong , Luo Ji-long , Yu Hong-xiang , Bai Chong-sheng , Han Yan-fei , Xia Shi-liang , Li Yan-fei
      To investigate the effects of aluminum trichloride (AlCl3) on renal structure and function in chickens, the chickens were injected continuously with different gradient AlCl3 for 60 days to establish sub-chronic aluminum (Al) intoxication model. The Al concentration in serum and kidney, contents of creatinine (Cr) and blood urea nitrogen (BUN) in serum were detected, and the renal structure was also observed by optical microscope. The results showed that the Al concentration in serum and kidney, the contents of Cr and BUN in serum were significantly higher in Al-treated chickens than those in the control group (P<0.05; P<0.01) and there was an AlCl3 dose-depended manner. Histopathologically, in the low dose group, acinus renis swelled mildly and there were no apparent pathological changes in nephric tubule and proximal convoluted tubule. In the middle and high dose group, acinus renis swelled, glomerular cells increased, nephric tubule cloudy swelled, and epithelial cells of proximal convoluted tubule swelled. The results indicated that sub-chronic Al exposure impaired the renal structure and function in chickens.


      PubDate: 2013-05-25T09:45:23Z
       
  • Optimizing Rank of Landscape Planning Works of Urban Wetland Park
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Qiao Li-fang , Zhang Yi-chuan , Qi An-guo , Li Xin-zheng
      Classifying and ranking the huge amounts of landscape planning works of urban wetland park is always difficult due to the multi-functions (ecological, leisure, educational and disaster prevention) of the urban wetland park. Therefore, an optimizing rank system is urgently needed. Analytic Hierarchy Process (AHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) models were used to rank the planning works of 30 urban wetland park based on four mainly factors, which included landscape ecological planning, landscape planning, ecological planning and economic planning. The study indicated that the AHP-TOPSIS model had good discrimination in the classification and ranking of landscape planning works of urban wetland park and it was also applicable to the planning works of other urban greenbelts.


      PubDate: 2013-05-25T09:45:23Z
       
  • Analysis on Stability Factors of Grain Price in China
    • Abstract: Publication date: September 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 3
      Author(s): Li Cui-xia , Zhang Yu-ling
      To make grain price stable is an important goal for the Chinese government. The paper compared the grain supply elasticity and demand elasticity to determine the grain price stability in China; used “k value” method to analyze the grain price fluctuation from 1985 to 2010; divided the grain price volatility into three stages; and analyzed the factors in each phase. On the base, it put forward some countermeasures to guarantee the stability of the grain price.


      PubDate: 2013-05-25T09:45:23Z
       
  • Inheritance of Erucic Acid, Glucosinolate, and Oleic Acid Contents in
           Rapeseed (Brassica napus L.)
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Huang Ze-su , Laosuwan Paisan , Machikowa Thitiporn , Chen Ze-hui , Dai Wen-dong , Tang Rong , Li Dezhen
      This study was conducted to verify the inheritance of certain characters of rapeseed including erucic acid, glucosinolate and oleic acid contents by using generation mean analysis. The cross of lines III174×Zi20 (F1), F2, BC1 (F1×P1), BC2 (F1×P2), and parents (P1 and P2) were evaluated in the field. Data were measured on individual plants for oleic acid, erucic acid, and glucosinolate contents. Transgressive variations in F2 population were observed for oleic acid content, indicating that dominance and recessive genes distributed in both parents. Scaling test indicated that the effects of genes controlling these characters did not follow the additive-dominance model. The data for three characters were analyzed using six parameter models and found that one or more types of epistatic gene effects were important for glucosinolate content. High broad sense heritabilities were obtained for erucic acid, oleic acid, and glucosinolate contents with the values of 98.97%, 93.68%, and 86.17%, respectively. Two major gene pairs were found to control the expression of erucic acid and oleic acid contents, while three major gene pairs were detected to control glucosinolate content.


      PubDate: 2013-05-25T09:45:23Z
       
  • Genetic Diversity and Global Distribution of Citrus tristeza virus (CTV)
           Strains
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Wu Xiao-yun , Cheng Xiao-fei , Luo Lu , Wu Xiao-xia
      Citrus tristeza virus (CTV), the most devastating viral pathogen in citrus, causes tremendous economic losses to citrus industry worldwide. The CTV isolates exhibit variable pathogenicities on their hosts indicating a mixed population of the CTV in nature. Several fragments within the CTV genome have been used for studying the genetic diversity of the CTV, however, the best region for rapid the CTV strain differentiation is still absent at present. In present study, a systemic analysis was carried out to evaluate the best region within the CTV genome for rapid CTV strain differentiation. Results of our study showed that the major coat protein (CP) coding region was the best region for this purpose. Using pair-wise distance frequency distribution plot, a reasonable genetic distance cut-off value was set for the CTV CP gene for the CTV strain differentiation. Using this criterion, eight CTV strains, including seven well characterized and a new strain, were successfully differentiated using 537 CTV isolates reported from 38 countries. The global strain distribution pattern was then determined and discussed. Our results also provided a new insight into the evolution and spreading of the virus, as well as the information for developing proper disease management strategy.


      PubDate: 2013-05-25T09:45:23Z
       
  • Evaluation and Genetic Analysis of Five Parental Varieties Resistant to
           Rice Blast Pathogen in Heilongjiang Province
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Zhang Zhong-chen , Liu Hong-liang , Gao Hong-xiu , Liu Hai-ying , Jin Zheng-xun
      Five F2 segregation populations, derived from crosses between the susceptible japonica cultivars (cvs.) Kongyu131 and donor cvs. Aichi Asahi (AA), BL1, Digu, Pai-kan-tao (PKT) and Oryzica Llanos 5 (ORL5), were used to evaluate their natural resistance to blast in cold region. The field test of their blast resistance was conducted in 858 Farm, which showed that Aichi Asahi, BL1and Digu were highly resistant to either leaf blast or neck blast and could be used to develop molecular breeding by design, and genetic analysis indicated that the field resistance of Aichi Asahi, BL1and Digu to leaf blast and neck blast was controlled by a single dominant gene, and the leaf blast and neck blast resistance in the donor cv. Pai-kan-tao was inherited as a single recessive gene, the neck blast resistance of the donor cv. Oryzica Llanos 5 was controlled by a single recessive gene while its leaf blast resistance was not controlled by this gene. These results suggested that five parental varieties positively contributed to resistance to either leaf blast or neck blast and could be used to expand the genetic germplasms resistant to blast in cold region using molecular assisted selection.


      PubDate: 2013-05-25T09:45:23Z
       
  • A Novel Method Isolated Microorganisms in Soil Granule
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Liu Bao-ping , Xiang Wen-sheng , Wang Hong-yan , Fu Shi-cong
      A novel method isolated microorganisms in soil granule was built. The key steps included: repeated elutriation of soil by sterilized water, inoculation on the plates with the elutriated sediments, incubation of the plates and isolation of the actinomycetes by using selected culture medium. We formulated that most microflora included the dominant actinomycetes in the soil were carried away with the sterilized water in the elutriation procedure, some rare actinomycetes and few other microflora included bacteria were remained in the elutriated sediments, the other microflora were excluded to grew into colonies on the plates by using selective culture medium for actinomycetes in the elutriated sediments. Results showed the supposition. Non-streptomycete actinomycetes were isolated both from black soil samples from Chinese northeast area and compost samples from Chinese central area. Soil fungi in granule were isolated by using the selective conditions to favor fungi. The results showed that the method was effective.


      PubDate: 2013-05-25T09:45:23Z
       
  • 3′ Noncoding Region Construction of GHR Gene-luciferase Report
           Vector and Valuation
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Jie Jing , Men Jing , Wang Chun-mei , Gao Xue-jun , Li Qing-zhang
      To analyze miR-139 target sites in 3′ UTR of GHR gene in dairy cow mammary gland, a GHR 3′ UTR- luciferase reporter vector was constructed and the effect of miRNA on its activity was evaluated in dairy cow mammary gland epithelial cells (DCMECs). The miR-139 targeting GHR 3′ UTR was predicted by Target Scan 5.1 software, 3′ UTR fragment of GHR was amplified by PCR from RNA of DCMECs. PCR products were cloned into Spe I/Hind III modified pMIR-Report vector. The luciferase reporter vector and miRNA eukaryotic expression vector were transferred into DCMECs using lipofectamine 2000 transfection reagent. The dualluciferase reporter assay system was used to quantitiate the reporter activity. The results showed that a 107 bp 3′ UTR fragment of GHR gene was successfully cloned into the pMIR-Report vector, which authenticated by Spe I/Hind III digestion and DNA sequencing. The luciferase activity of reporter construction treated with miR-139 decreased 20.87% compared with the control group. It was concluded that the GHR3′ UTR-luciferase reporter vector had been successfully constructed. The luciferase activity of the reporter could be suppressed by miR-139.


      PubDate: 2013-05-25T09:45:23Z
       
  • PiggyBac Transposon Mediated Efficient eGFP Expression in Porcine Somatic
           Cells and Cloned Embryos
    • Abstract: Publication date: June 2012
      Source:Journal of Northeast Agricultural University (English Edition), Volume 19, Issue 2
      Author(s): Luo Yi-bo , Zhang Li , Zhu Jiang , Wu Mei-ling , Huan Yan-jun , Yin Zhi , Mu Yan-shuang , Xia Ping , Liu Zhong-hua
      PiggyBac transposon has demonstrated its long-term and stable transposition on genomes of various species but lacking of the evidence on farm animal genomes. In this study, we constructed a piggyBac transposon marked with enhanced green fluorescent protein (eGFP) and showed efficient transposition in porcine somatic cells and cloned embryos. Our results demonstrated that piggyBac transposase could efficiently catalyze transposition in porcine fetal fibroblast cells, as well as in embryos. PiggyBac transposition generated 18-fold more eGFP-positive cell colonies compared to pEGFP-C1 random insertion mutagenesis, but excessive transposase might affect the transfection rate. Also piggyBac mediated 4-fold more eGFP expression than random insertion in cells and 17-fold in cloned embryos at mRNA level. When the mutagenized cells were used for somatic cell nuclear transfer (SCNT), the cleavage rate and blastocyst rate of constructed embryos harboring piggyBac transposition had no difference with random insertion group. This study provides key information on the piggyBac transposon system as a tool for creating transgenic pigs.


      PubDate: 2013-05-25T09:45:23Z
       
 
 
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