Authors:Sitti Saleha, Nurdin Saidi, Saiful ., Murniana ., Saida Rasnovi, Teuku M. Iqbalsyah Pages: 1 - 6 Abstract: Proximate analysis of Dioscorea hispida tubers, collected from five locations around Leuser ecosystem in Aceh Province, showed variations amongst samples. Standard AOAC method for proximate analysis of the fresh weight showed that the water content varied between 15.8 - 37.8%, crude protein 1.13 -6.20%, crude lipid 1.99 - 9.36% and ash 0.29 - 1.24%. The total carbohydrate was high, i.e. between 58.3 -71.9%. The main mineral was phosphorus, with a value of 11.7 - 46.9 mg/100g. These variations could be due to soil, climate and weather factors, as well as postharvest handling. Phytochemical tests showed that all of the samples contained alkaloids and terpenoids. One of the samples (LP) also contained phenol and steroid. The high cyanide content in the tubers (379 - 739 ppm) was easily removed by repeated washing. The cyanide level dropped significantly after the 3rd wash. Information on nutritional content in D. hispida is essential for planning its utilization. Increasing the economic value of D. hispida is expected to attract people around the Leuser ecosystem to cultivate and utilize it, thereby reducing illegal forest encroachment.Keywords: Dioscorea hispida, proximate, Leuser, janeng, gadung, starchREFERENCES Barton H 2014 Yams: Origins and Development, Encyclopaedia of Global Archaeology, p 7943-7947, (Springer. DOI 10.1007/978-1-4419-0465-2_2193). Obidiegwu J E and Akpabio E M 2017 The Geography of Yam Cultivation in Southern Nigeria: Exploring Its Social Meanings and Cultural Functions J. Ethnic Foods 4 28-35. Chandrasekara A and Kumar T J 2016 Roots and Tuber Crops as Functional Foods: A Review on Phytochemical Constituents and Their Potential Health Benefits Intl. J. Food Sci. 2016 1-15. Kumar S, Das G, Shin H-S and Patra J K 2017 Dioscorea spp. (A Wild Edible Tuber): A Study on Its Ethnopharmacological Potential and Traditional Use by the Local People of Similipal Biosphere Reserve India Front. Pharmacol. 8 52. Lin J Y, Lu S, Liou Y L and Liou H L 2006a Antioxidant and Hypolipidaemic Effects of a Novel Yam–boxthorn Noodle in an In Vivo Murine Model Food Chem. 94 377–384. Lin J Y, Lu S, Liou Y L and Liou H L, 2006b, Increased IgA and IgG Serum Levels Using a Novel Yam–boxthorn Noodle in a BALB/c Mouse Model Food Chem. Toxicol. 44 170–178. Bhandari M R and Kawabata J 2004 Organic Acid, Phenolic Content and Antioxidant Activity of Wild Yam (Dioscorea spp.) Tubers of Nepal Food Chem. 88 163–168. Lin J T and Yang D J 2008 Determination of Steroidal Saponins in Different Organs of Yam (Dioscorea pseudojaponica Yamamoto) Food Chem. 108 1068–1074. AOAC 1984. Official Methods of Analysis. Washington DC: Association of Official Analytical Chemists. Harborne J B 1984 Phytochemical Method 2nd ed. (London: Chapman and Hall Ltd.) Bhandari M R, Kasai T and Kawabata J 2003 Nutritional Evaluation of Wild Yam (Dioscorea spp.) Tubers of Nepal Food Chem. 82 619–623. Adepoju O T, Boyejo O and Adeniji P O 2017 Nutrient and Antinutrient Composition of Yellow Yam (Dioscorea cayenensis) Products Data in Brief 11 428–431. Wu Z-G, Jiang W, Nitin M, Bao X-Q, Chen S-L and Tao Z-M 2016 Characterizing Diversity Based on Nutritional and Bioactive Compositions of Yam Germplasm (Dioscorea spp.) Commonly Cultivated in China J. Food Drug Anal. 24 367 – 375. Udensi E A, Osebele H O and Iweala O O 2008 The Investigation of Chemical Composition and Functional Properties of Water Yam (Dioscorea alata): Effect of Varietal Differences Pakistan J. Nutrition 7(2) 342-344. Hornick S B 1992 Factors Affecting the Nutritional Quality of Crops Am. J. Altern. Agric. 7 (Special Issue on Soil Quality) 63-68. Lewicki P P 2004 Water as the Determinant of Food Engineering Properties, A Review J. Food Eng. 61 483–495 Yeh A-I, Chan T-Y and Chuang G C-C 2009 Effect of Water Content and Mucilage on Physico-chemical Characteristics of Yam (Discorea alata Purpurea) Starch J. Food Eng. 95 106–114. McPherson E and Jane J 1999 Comparison of Waxy Potato with Other Root And Tuber Starches Carbohydr. Polym. 40 57–70. Freitas R A, Paula R C, Feitosa J P A, Rocha S and Sierakowski M R 2004 Amylose Contents, Rheological Properties and Gelatinization Kinetics of Yam (Dioscorea alata) and cassava (Manihot utilissima) starches Carbohydr. Polym. 55 3–8. Barsby T L, Donald A M, Frazier P J, Donald A M, Perry P A and Waigh T A 2001 The Impact of Internal Granule Structure on Processing and Properties in Starch: Advances in Structure and Function p 45-52 (Royal Society of Chemistry, http://dx.doi.org/10.1039/9781847551917-00045) Tattiyakul J, Naksriarporn T and Pradipasena P 2012 X-ray Diffraction Pattern and Functional Properties of Dioscorea hispida Dennst Starch Hydrothermally Modified at Different Temperatures Food Bioproc. Technol. 5 964–971. Savikin-Fodulovic K, Grubisic D, Culafic L, Menkovic N and Ristic M 1998 Diosgenin and Phytosterols Content in Five Callus Lines of Dioscorea balcanica Plant Sci. 135 63–67. Cushnie T. P. T, Cushnie B and Lamb A J 2014 Alkaloids: An Overview of Their Antibacterial, ... PubDate: 2018-02-01 Issue No:Vol. 18, No. 1 (2018)
Authors:Ridha kurniati, Nurdin Saidi, Rosnani Nasution Pages: 7 - 10 Abstract: Antifeedant activity of neem leaf (A. indica A. Juss) has been identified by against Tenebrio molitor bio-indicator. The highest activity was obtained on ethyl acetate extract at 0.5% concentration having Antifeedant Index (AI) of 51.53% and most active at 10% concentration of 82.05%. The method used to test the antifeedant activity is the no choice leaf disk method. Secondary metabolites contained in neem leaf extract (A. indica A. juss) include terpenoids, steroids, flavonoids, saponins and phenolics. Keywords: Neem leaf (Azadirachta Indica A.Juss), No choice leaf disk method, Antifeedant ActivityREFFERENCES Benge, M.D. 1986. Neem the Cornucopia Tree. S and T/FENR Agroforestation Technical Series No. 5. Agency for International Development Washington, D.C.190p. Schumutterer., H. 2002. Properties and Potensial of Natural pPsticides from Neem Tree, Azadirachta indica Ann. Rev. Entomol. 35; 271-291 Alzohairy, M.A. 2016. Review Article Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment, Article ID 7382506, 11p. 4. Patel, S.M., Venkata., K.C.N., Bhattacharyya, P., Sethi, G., Bishayee, A. 2016. Potential of Neem (Azadirachta indica) For Prevention and treatment of Oncologic Diseases Seminar In Cancer Biology Diabate, D., Gnago, J.A., Tano, Y., 2014. Toxicity, Antifeedant and Repellent, effect of Azadirachta indica A. Juss and Jatropa carcus L. aqueous extracts agaianst Plutella xylostella (Lepidoptera: Plutellidae) J. Basic. Appl. Sci. Res. 4 (11) : 51 – 60 Jeyasankar, A., and Gokilamani, D., 2016. Biology and eco-friendly control of Amaranth pests, Hymenia recurvalis Fabricus and Psara basalis (Lepidoptera : Crambidae) Inter. J. Acad. Stud. 2 (4): 218 – 230. Pavunraj, M., Muthu, C., Ignacimuthu,S., Janarthanan, S., Duraipandiyan, V., Raja, N. and Vimalraj, S. 2011. Antifeedant Activity of a Novel 6-(4,7-hydroxyl-heptyl)Quinone From The Leaves of The Milkweed Pergularia daemia on The Cotton Bollworm Helicoverpa armegera (Hub.) and The Tobacco Armworm Spodoptera litura (Fab.) Phytoparasitica 39 : 145 – 150. Munoz, E., Lamilla, C., Marin, J.C., Alarcon, J., Cespedes, C.L.m 2012. Antifeedant, Insect Growth and Insecticidal Effec of Calceolaria talcana (Calceolariaceae) on Dropsopphila melanogaster and Spodoptera frugiperda Industrial Crops and Product, 42, 137 – 144. Saxena, M., Saxena, J., Nema, R., Singh, D and Gupta, A. 2013. Phytochemistry of Medicinal Plants Journal pharmacognosy and Phytochemistry, 1;6. Liu, L., Zhao, Y.L., Cheng, G.G., Chen, Y.Y., Qin, angX.J., C.C.W., Yang, X.W., Liu, Y.P., Luo, X.D, 2014. Antifeedant activity and effect of fruits and seed extract of Cabralea canjerana canjerana (Vell.) Mart. (Meliaceae) on the immature stages of the fall armworm Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae Crops and Product. 65 ; 156 – 158. Schoonhven, LM. 1982. Biologycal Aspect of Antifeedant . Ent, Exp and Appl .31: 57 - 69 Gahukar, R.T. 2014. Factor Affecting Content and Bioefficacy of Neem (Azadirachta indica A. Juss) Phytochemicals used in Agriculture pest control A Review. Crop Protection. 62: 93 – 99. Pattanaik, S.J., Ranghupati, N.D.,Chary, P.2006.Ecomorphometric Marker Reflect Variation in Azadirachtin Contents of Azadirachta indica A. Juss. (Meliaceae) in Select Regions of Andhra Pradesh India. Curr. Sci, 91:628 – 636. Adel, M.M., Sehnal, H., Jurzysta, M. 2000. Effects of Alfalfa safonin on the Moth Spodoptera litura Journal of Chemical Ecology, 26 : 7-14 Shuklar, Y.N., Rani, A. Tripathi,A.K., Sharma, S. 1996. Antifeedant, Activity of Ursolic Acid Isolation from Duboisia myoporoides Phytotheraphy, 10 : 359 – 360 Walter, J.F. 1999. Commercial With Neem Product, P. 155-170. In Franklin R, Hall and Julius J. Menn. Biopesticides Use and Delivery. Humana Press. Totowo, New Jersey. PubDate: 2018-01-24 DOI: 10.24815/jn.v18i1.8781 Issue No:Vol. 18, No. 1 (2018)
Authors:Binawati Ginting, Ratna Maira, Mustanir ., Hira Helwati, Lydia Septa Desiyana, Rohmat Mujahid Pages: 11 - 17 Abstract: The essential oil of the nutmeg plant (Myristica fragrans Houtt) has been obtained from roots, bark, fruit, mace and seeds using Stahl steam distillation. Each essential oil have tested for antioxidant activity with DPPH. Antioxidant activity of essential oil from each nutmeg plant to DPPH with concentration 25, 50 and 100 ppm. Each essential oil did not show strong antioxidant activity but the result of nutmeg isolation had strong antioxidant activity with IC50 that was 80,555 ppm. Based on GC-MS analysis of essential oil of nutmeg isolate obtained myristicin compound with 96.52% area and time Retention 22.127. Myristicin is one of the main components of essential oils of nutmeg plants that play an important role as antioxidants. Keywords: Nutmeg plant (Myristica fragrans Houtt), Essential Oil, DPPH, Antioxidant radicalsREFERENCES Suryanti, Siti. 2014. Metabolit Sekunder Pada Tanaman dan Fungsinya. http://seputarduniasains.blogspot.com Diakses tanggal 2 juni 2015. Gupta, A. D., Bansal, V. K., Babu, V., Maithil, N. 2013. Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fregrans Houtt) Journal of Genetic Engineering and Biotechnologyh 11:25-31. Rodianawati, I., Hastuti, P., Cahyanto, M. N. 2015. Nutmeg’s (Myristica fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties Procedia Food Science 3:244-254. Masyithah, Z. 2006. Pengaruh Volume dan Konsentrasi Pelarut pada Isolasi Trimiristin dari Limbah Buah Pala Jurnal Teknologi Proses 5(1) Januari; 64-67. Wibowo, S dan Komarayati, S. 2015. Sifat Fisiko Kimia Minyak Cupresus (Cupressus benthamii) Asal Aek Nauli, Parapat Sumatera Utara Jurnal Penelitian Hasil Hutan 33 (2) Juni 2015: 93-103. Nurdjannah, N. 2007. Teknologi Pengolahan Pala. Badan Penelitian dan Pengembangan Pertanian. Bogor. Hellen, M, Vargheese, T.N, Kumari, J, Abiramy, Sajina, Sree, J, 2012, Phytochemical Analysis and Anticancer Activity of Essential Oil From Myristica fragrans, International Journal of Current Pharmaceutical Review and Research. Chatterjee, S, Zareena Niaz, S. Gautam, Soumyakanti Adhikari, Prasad S. Variyar, Arun Sharma, 2007, Antioxidant Activity of Some Phenolic Constituents from Green Pepper (Piper nigrum L.) and Fresh Nutmeg Mace (Myristica fragrans) J. Food Chemistry 101, 515–523. Sulaiman, S. F and Kheng, L. O. 2012. Antioxidant and anti food-borne bacterial activities of extracts from leaf and different fruit parts of Myristica fragrans Houtt. Food Control 25:533-536. Akinboro, A, Kamaruzzaman, M. B, Asmawi, M. Z, Sulaiman, S. F, Sofiman, O. H. 2011. Antioxidans in Aqueous extract of Mirystica fragrans (Houtt) Suppress Mitosis and Cyclophosphamide-induce chromosomal aberrations in Allium cepa L. Cells, Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology). ISSN 1673-1581 (Print); ISSN 1862-1783 (Online).www.zju.edu.cn/jzus; www.springerlink.com Ginting, B., 2013, Aktifitas Antifungi Ektrak Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia tahun 2013. Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2013, Isolasi dan Sifat Antioksidan Total Flavonoid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia, Samarinda Ginting, B, T. Barus, P, Simanjuntak, L. marpaung.,2013, Isolasi dan Dan Penentuan Aktivitas Antioksidan Total Alkaloid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Yusuf Banseh Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2016, Isolation and Identification of Flavonoid Compound from Nutmeg Leaves (Mirystica fragrans Houtt), Asian Journal Of Chemistry. Ginting, B., Mustanir., Helwati, H., Desiyana, L. S., Eralisa, Mujahid, R. 2017. Antioxidant Activity Of N-Hexane Extract Of Nutmeg Plants From South Aceh Province. Jurnal Natural 17(1) Ramaswany, V. N., Varghese, A., Simon. 2011. An Investigation on Cytotoxic Ternatea L International Journal of Drug Discovery 3: 74-77. ISSN : 0975-4423. Guenther, E. 1987. Minyak Atsiri Jilid 1, terjemahan dari: Essensial oil. Penerjemah: Ketaren S, Universitas Indonesia Press, Jakarta. Harborne, J.B. 1987. Metode Fitokimia: Penentuan Cara Moderen Menganalisa Tumbuhan. Terjemahan dari Phytochemical Methods oleh Kosasih Padmawinata. ITB. Bandung. Sipahelut, S. G. 2012. Karakteristik Minyak Daging Buah Pala (Myristica fragrans Houtt) Melalui Beberapa Cara Pengeringan Dan Distilasi Journal Agroforestri 7(1) Maret 2012. Andini, V., Gupta, S., Chatterejee, S., Variyar, P.S and Sharma, A. 2015. Activity Guided Characterization of Antioxidant Components from Essential Oil of Nutmeg (Myristica fragrans). Vol 52; 221-230. Nagja, T., Vimal, K, Sanjeev, A. 2015. Myristica fragrans: A Comprehensive Review International Journal of Pharmacy and Pharmaceutical Sciences 8 (2). Isnindar., Wahyuono, S., Setyowati, E.P. 2011. Isolasi dan Identifikasi Senyawa Antioksidan Daun Kesemek (Diospyros ka... PubDate: 2018-02-01 DOI: 10.24815/jn.v18i1.6604 Issue No:Vol. 18, No. 1 (2018)
Authors:Syafrizal Idris, Muhammad Syukri, Muhammad Syukri Surbakti, Marwan ., Muchlis ., Ibnu Rusydy, Nurul Aflah Pages: 18 - 21 Abstract: It has been conducted a geo-electrical resistivity survey at geothermal area of Ie Jue, Aceh Besar. The survey aims to measure resistivity value in the area. It is expected that the values of resistivity will image the subsurface structure in the area. In addition geological features in the area are shown after we process it. The data acquisition in this study was acquired using a SuperSting R8/IP resistivitymeter and its data processing using EarthImager 2D software to get the image of resistivity pseudosection. The two lines of measurement crossed the surface geothermal manifestation. Each line has 330 m in length, and 56 electrodes with electrode space of 6 m. The result of resistivity pseudosection model interpreted based on geological condition and observation in the area. Resistivity pseudosection model shows the study is water-saturated (<64.0 Ωm) to a depth of ±65 m and a highly conductive zone (0.6-3.5 Ωm) with a thickness of 30-50 m. This conductive layers below the embodied surface at a distance of 158-160 m on the IJ-Y1 line and at a range of 130-185 m on the IJ-Y2 line. This conductive zone is indicated as a weak zone where geothermal fluids are transported to the surface.Keywords: Geothermal, Ie Jue, geo-electrical resistivity survey, resistivityREFERENCES Bennet, J.D., Bridge, D.McC., Cameron, N.., Djunuddin, A., Ghazali, S.A., Jeffrey, D.H., Kartawa, W., Rock, M.N.S., Thomson, S.J., Whandoyo, R., 1981. PetaGeologiLembar Banda Aceh, Sumatera, Puslitbang Geologi. Bandung. Syukri, M., Saad, R., Nordiana, M., & Azwin, I. 2014. Preliminary Study of Sumatera Fault Using 2-D Resistivity Imaging Method. Electronic Journal of Geotechnical Engineering, 19. Knödel, K., Lange, G., & Voigt, H.J., 2007. Environmental geology: Handbook of field methods and case studies: Springer Science & Business Media. Telford, W. M., Geldart, L. P., Sheriff, R. E. 1990. Applied Geophysics Second Edition, Cambridge University Press, USA Marwan, M., Rusydy, I., Nugraha, G.S. and Asrillah, A., 2014. Study of Seulawah Agam’s Geothermal Source Using Gravity Method. Jurnal Natural, 14(2) Asrillah, A., Marwan, M., Rusydy, I. and Nugraha, G.S., 2014. Application of Magnetics Method to Mapping the Geothermal Source at Seulawah Agam Area. Jurnal Natural, 14(2). Ismail, N.,Ramadhan, S., 2013. Karakterisasi Struktur Dangkal pada Lapangan Panas Bumi Seulawah Agam Menggunakan Metode Very Low Frequency (VLF). Prosiding Semirata FMIPA Universitas Lampung. Lampung, Indonesia. PubDate: 2018-02-03 DOI: 10.24815/jn.v18i1.9676 Issue No:Vol. 18, No. 1 (2018)
Authors:Ismail ., Akmal Nizar, Mursal . Pages: 22 - 25 Abstract: Abstract. Cempaka-madu is a local name for one kind of gemstone from Aceh province in Indonesia. It is so attractive and its color is dark-orange. We have utilized X-Ray Florescent (XRF) to study these cempaka-madu gemstones from Aceh Tengah and Nagan Raya districts in Aceh province. Our results show that the cempaka-madu gemstone from Nagan Raya district contains 84.90% of SiO2, 8.89% of NiO, 5.47% of Fe2O3, and 0.6% of CaO. The mineral contains in cempaka-madu gemstone from Aceh Tengah district is about the same as those from Nagan Raya district. It is found that the oxide compounds contained in the cempaka-madu gemstone is significantly different than that in jadeite, nephrite-actinolite, nephrite-tremolite, serpentine-clinochrysotile, serpentine-antigoride, and vesuvianite. Consequently, we conclude that the cempaka-madu gemstone from Aceh Indonesia cannot be categorized as jade. Keywords: cempaka-madu gemstone, jade, jadeite, nephrite, serpentine, vesuvianite PubDate: 2018-02-07 DOI: 10.24815/jn.v18i1.9802 Issue No:Vol. 18, No. 1 (2018)
Authors:Erda Marniza, Marlina ., M Nasir Pages: 26 - 31 Abstract: Abstract. The effect of bentonite as filler on the performance of the composite membrane of PVDF-PMMA has been performed. This study was conducted to determine the performance PVDF-PMMA composite membranes and membrane applications PVDF-PMMA-Bentonite on oily wastes. Bentonite is obtained from North Aceh while PVDF membrane-PMMA by phase inversion method. This study uses an oily waste water model was made by mixing surfactant tween 80 with concentration of 2% with palm oil and gas oil. Membrane PVDF-PMMA-Bentonite is analyzing using cell ultrafiltration (flux test), FTIR and SEM-EDX. The results showed that the value of flux with the addition of bentonite is greater than without bentonite. Good flux values contained in the PVDF-PMMA-Bentonite (1:1:2) amounted to 32.143 L/m2.h.bar with permeability of 21.428 L/m2.h. FTIR characterization analysis results show that bentonite can add to pore at wave number 1520 cm -1 and 1660 cm-1 SEM and EDX results showed regular shape and uniform pore. Keywords: Membrane, bentonite, filler, FTIR, SEM-EDX, Oily wastewater PubDate: 2018-02-13 DOI: 10.24815/jn.v18i1.8023 Issue No:Vol. 18, No. 1 (2018)
Authors:Sadli . Pages: 32 - 37 Abstract: Abstract. A research on analyzing the phytochemical content in Volvariella volvacea (straw mushroom) extract from a local cultivation in Aceh has been conducted. Straw mushroom is potentially a medicine ingredient. This study aims to examine the secondary metabolite compounds in the methanol extract of straw mushroom. The extraction of the secondary metabolite compounds was undertaken by using the maceration method with methanol solvent. Phytochemical test was conducted qualitatively by using the meyer’s reagent, bouchardat, dragendrof, FeCl3, HCl 0.1 M, HCl 2N and Lieberman – bouchardat. Phytochemical analysis was carried out on flavonoids, alkaloids, glycosides, saponins, tannins and terpenoids. The phytochemical analysis’ result shows that the straw mushroom extract contains alkaloids, flavonoids, terpenoids, and saponins.Keywords: straw mushroom, methanol extract, maceration method, phytochemical screening PubDate: 2018-02-13 DOI: 10.24815/jn.v18i1.9228 Issue No:Vol. 18, No. 1 (2018)
Authors:Surya Lubis, Ilham Maulana, Masyithah . Pages: 38 - 43 Abstract: In this paper, TiO2/α-Fe2O3 composite with high photocatalytic activity was prepared by a mechanical milling using iron sand from Lampanah in Aceh Besar regency as hematite (α-Fe2O3) source. Hematite was extracted from iron sand by using hydrochloric acid followed by co-precipitating using ammonium hydroxide as co-precipitation agent. Hematite and TiO2 powder were mixed and milled on a planetary ball mill without incorporating any solvent. The materials were characterized by using X-ray difraction (XRD) and scanning electron microscopy energy dispersive x-ray spectroscopy (SEM-EDX). The XRD results showed that the iron (III) oxide synthesized was hematite (α-Fe2O3) phase with the average crystallite size 27.967 nm. SEM analysis showed that iron sand was on irregular form, while the hematite (α-Fe2O3) and TiO2/α-Fe2O3 composite was on regular sphere. The TiO2/α-Fe2O3 composite was evaluated on photodegradation of indigo carmine (IC) dye using UV light irradiation. The highest degradation efficiency of IC (100%) was obtained by initial pH of dye solution equal to 1, photocatalyst dosage of 0.2 g, initial dye consentration of 5 mg/L for 120 min irradiation time. The photocatalytic activity of TiO2/α-Fe2O3 composites using UV light was almost the same as that of by solar light. Keywords: Iron sand, α-Fe2O3/TiO2 composite, mechanical milling, photocatalyst, indigo carmine PubDate: 2018-03-03 DOI: 10.24815/jn.v18i1.8649 Issue No:Vol. 18, No. 1 (2018)
Authors:Kartini Eriani, Ainsyah Ainsyah, Rosnizar Rosnizar, Yunita Yunita, Ichsan Ichsan, Al Azhar Pages: 44 - 48 Abstract: Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p<0.05) between treatment groups. Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation. PubDate: 2018-04-30 DOI: 10.24815/jn.v18i1.9830 Issue No:Vol. 18, No. 1 (2018)
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