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  Subjects -> CONSERVATION (Total: 140 journals)
Showing 1 - 37 of 37 Journals sorted alphabetically
Advanced Sustainable Systems     Hybrid Journal   (Followers: 4)
African Journal of Ecology     Hybrid Journal   (Followers: 16)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 13)
African Journal of Wildlife Research     Full-text available via subscription   (Followers: 7)
AICCM Bulletin     Hybrid Journal   (Followers: 1)
Ambiens. Revista Iberoamericana Universitaria en Ambiente, Sociedad y Sustentabilidad     Open Access   (Followers: 1)
American Journal of Rural Development     Open Access   (Followers: 7)
American Museum Novitates     Open Access   (Followers: 5)
Animal Conservation     Hybrid Journal   (Followers: 43)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 7)
Aquatic Conservation Marine and Freshwater Ecosystems     Hybrid Journal   (Followers: 41)
Arcada : Revista de conservación del patrimonio cultural     Open Access   (Followers: 1)
Archeomatica     Open Access   (Followers: 9)
Arid Land Research and Management     Hybrid Journal   (Followers: 10)
Asian Journal of Sustainability and Social Responsibility     Open Access   (Followers: 4)
Australasian Plant Conservation: Journal of the Australian Network for Plant Conservation     Full-text available via subscription   (Followers: 4)
Biodiversity and Conservation     Open Access   (Followers: 244)
Biological Conservation     Hybrid Journal   (Followers: 377)
Business Strategy and the Environment     Hybrid Journal   (Followers: 13)
Catalysis for Sustainable Energy     Open Access   (Followers: 11)
Challenges in Sustainability     Open Access   (Followers: 8)
Chelonian Conservation and Biology     Hybrid Journal   (Followers: 9)
Conservación Vegetal     Open Access  
Conservation     Hybrid Journal   (Followers: 28)
Conservation Biology     Hybrid Journal   (Followers: 335)
Conservation Genetics     Hybrid Journal   (Followers: 19)
Conservation Science     Open Access   (Followers: 25)
Conservation Science and Practice     Open Access  
Diversity and Distributions     Open Access   (Followers: 44)
Earth's Future     Open Access   (Followers: 3)
Eastern European Countryside     Open Access   (Followers: 2)
Eco-Entrepreneur     Open Access  
Ecological Economics     Hybrid Journal   (Followers: 199)
Ecological Psychology     Hybrid Journal   (Followers: 3)
Ecological Restoration     Full-text available via subscription   (Followers: 22)
Ecology and Evolution     Open Access   (Followers: 95)
Ecology and Society     Open Access   (Followers: 51)
Environment and Natural Resources Journal     Open Access  
Environmental and Resource Economics     Hybrid Journal   (Followers: 25)
Environmental and Sustainability Indicators     Open Access   (Followers: 3)
Environmental Conservation     Hybrid Journal   (Followers: 59)
Ethnobiology and Conservation     Open Access   (Followers: 3)
European Countryside     Open Access   (Followers: 1)
Forest Policy and Economics     Hybrid Journal   (Followers: 22)
Forum Journal     Full-text available via subscription   (Followers: 1)
Frontiers in Ecology and Evolution     Open Access   (Followers: 43)
Functional Ecology     Hybrid Journal   (Followers: 52)
Future Anterior     Full-text available via subscription  
Global Ecology and Biogeography     Hybrid Journal   (Followers: 74)
Global Ecology and Conservation     Open Access   (Followers: 16)
Human Dimensions of Wildlife: An International Journal     Hybrid Journal   (Followers: 6)
Ideas in Ecology and Evolution     Open Access   (Followers: 11)
In Situ. Revue des patrimoines     Open Access   (Followers: 1)
Indonesian Journal of Conservation     Open Access  
Indonesian Journal of Sustainability Accounting and Management     Open Access   (Followers: 1)
Interações (Campo Grande)     Open Access  
Interdisciplinary Environmental Review     Hybrid Journal   (Followers: 3)
International Journal of Architectural Heritage: Conservation, Analysis, and Restoration     Hybrid Journal   (Followers: 10)
International Journal of Biodiversity and Conservation     Open Access   (Followers: 4)
International Journal of Biodiversity Science and Management     Open Access   (Followers: 9)
International Journal of Environment and Pollution     Hybrid Journal   (Followers: 2)
International Journal of Global Energy Issues     Hybrid Journal   (Followers: 8)
International Journal of Social Ecology and Sustainable Development     Full-text available via subscription   (Followers: 10)
International Journal of Sustainable Development     Hybrid Journal   (Followers: 16)
International Journal of Sustainable Development and World Ecology     Hybrid Journal   (Followers: 13)
International Soil and Water Conservation Research     Open Access   (Followers: 3)
Intervención     Open Access   (Followers: 1)
Journal for Nature Conservation     Hybrid Journal   (Followers: 31)
Journal for the Study of Religion, Nature and Culture     Hybrid Journal   (Followers: 12)
Journal of East African Natural History     Full-text available via subscription   (Followers: 7)
Journal of Ecology and The Natural Environment     Open Access   (Followers: 4)
Journal of Industrial Ecology     Hybrid Journal   (Followers: 15)
Journal of International Wildlife Law & Policy     Hybrid Journal   (Followers: 4)
Journal of Natural Resources Policy Research     Hybrid Journal   (Followers: 10)
Journal of Paper Conservation     Hybrid Journal   (Followers: 3)
Journal of Rural Studies     Hybrid Journal   (Followers: 17)
Journal of Sustainable Mining     Open Access   (Followers: 3)
Journal of the American Institute for Conservation     Hybrid Journal   (Followers: 4)
Journal of the Institute of Conservation     Hybrid Journal   (Followers: 27)
Journal of Threatened Taxa     Open Access   (Followers: 2)
Journal of Urban Ecology     Open Access   (Followers: 5)
Julius-Kühn-Archiv     Open Access  
Lakes & Reservoirs Research & Management     Hybrid Journal   (Followers: 14)
Landscape and Urban Planning     Hybrid Journal   (Followers: 33)
Madagascar Conservation & Development     Open Access   (Followers: 3)
Madera y Bosques     Open Access  
Media Konservasi     Open Access   (Followers: 1)
Monographs of the Western North American Naturalist     Full-text available via subscription  
Natural Resources and Conservation     Open Access   (Followers: 5)
Natural Resources Forum     Hybrid Journal   (Followers: 7)
Nature Conservation     Open Access   (Followers: 36)
Nature Sustainability     Hybrid Journal   (Followers: 11)
Natureza & Conservação : Brazilian Journal of Nature Conservation     Open Access  
Neotropical Biology and Conservation     Open Access   (Followers: 2)
Nepalese Journal of Development and Rural Studies     Open Access  
Northeastern Naturalist     Full-text available via subscription   (Followers: 3)
Northwestern Naturalist     Full-text available via subscription  
Novos Cadernos NAEA     Open Access   (Followers: 1)
npj Urban Sustainability     Open Access   (Followers: 2)
Nusantara Bioscience     Open Access   (Followers: 4)
Ocean Acidification     Open Access   (Followers: 6)
One Ecosystem     Open Access  
Oryx     Hybrid Journal   (Followers: 18)
Pacific Conservation Biology     Full-text available via subscription   (Followers: 2)
Park Watch     Full-text available via subscription   (Followers: 1)
Proceedings of the Academy of Natural Sciences of Philadelphia     Full-text available via subscription   (Followers: 4)
Process Integration and Optimization for Sustainability     Hybrid Journal  
Rangeland Journal     Hybrid Journal   (Followers: 4)
Recursos Rurais     Open Access   (Followers: 1)
Recycling     Open Access  
Resources, Conservation & Recycling     Hybrid Journal   (Followers: 23)
Resources, Conservation & Recycling : X     Open Access   (Followers: 2)
Restoration Ecology     Hybrid Journal   (Followers: 47)
Revista de Ciencias Ambientales     Open Access   (Followers: 2)
Revista de Direito e Sustentabilidade     Open Access  
Revista Meio Ambiente e Sustentabilidade     Open Access   (Followers: 1)
Revista Memorare     Open Access  
Rural Sustainability Research     Open Access   (Followers: 4)
Savana Cendana     Open Access  
Society & Natural Resources: An International Journal     Hybrid Journal   (Followers: 20)
Socio-Ecological Practice Research     Hybrid Journal  
Soil Ecology Letters     Hybrid Journal  
Southeastern Naturalist     Full-text available via subscription   (Followers: 3)
Southern Forests : a Journal of Forest Science     Hybrid Journal   (Followers: 4)
Studies in Conservation     Hybrid Journal   (Followers: 14)
Sustainable Earth     Open Access  
Sustainable Environment Agricultural Science (SEAS)     Open Access  
Sustentabilidade em Debate     Open Access  
Tanzania Journal of Forestry and Nature Conservation     Full-text available via subscription   (Followers: 2)
The American Midland Naturalist     Full-text available via subscription   (Followers: 11)
The Southwestern Naturalist     Full-text available via subscription   (Followers: 3)
Tropical Conservation Science     Open Access   (Followers: 2)
Tropical Ecology     Hybrid Journal  
VITRUVIO : International Journal of Architectural Technology and Sustainability     Open Access  
Water Conservation Science and Engineering     Hybrid Journal   (Followers: 1)
Western North American Naturalist     Full-text available via subscription   (Followers: 1)
Wildfowl     Open Access   (Followers: 8)
Wildlife Australia     Full-text available via subscription   (Followers: 1)
Wildlife Research     Hybrid Journal   (Followers: 15)
World Review of Entrepreneurship, Management and Sustainable Development     Hybrid Journal   (Followers: 3)

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Nusantara Bioscience
Number of Followers: 4  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2087-3948 - ISSN (Online) 2087-3956
Published by Society for Indonesian Biodiversity Homepage  [11 journals]
  • Short Communication: Genetic diversity of the IGF2 gene as a source of
           genetic marker for halal authentication

    • Authors: ANTI DAMAYANTI HAMDANI, MADIHAH ., RR. BHINTARTI SURYOHASTARI, DEWI PETI VIRGIANTI, RAHAYU FITRIAYANI WANGSA PUTRIE
      Pages: 203 - 209
      Abstract: Hamdani AD, Madihah, Suryohastari RB, Virgianti DP, Putrie RFW. 2018. Genetic diversity of the IGF2 gene as a source of genetic marker for halal authentication. Nusantara Bioscience 10: 203-209. The main issue of halal authenticity is the availability of reliable and rapid analytical methods to identify animal species in raw and processed food. The two most popular procedures for identifying the source of the meat are protein-based and DNA-based methods, including mass spectroscopy (MS) using a peptide marker or PCR based DNA marker, respectively. This study aims to investigate the genetic diversity of insulin-like growth factor 2 (IGF2) gene, mainly from porcine (Sus scrofa) and bovine (Bos taurus) as a source of genetic marker for halal authentication by in-silico analysis using bioinformatic tools. Multiple sequence alignment and phylogenetic tree construction of IGF2 protein sequences from representative species of mammals, birds, and fishes showed the paraphyletic relationship between the IGF2 protein of S. scrofa and B. taurus. Protein structure analysis revealed differences in helical structures near the carboxyl end of the protein, while gene analysis showed different number of exon and motifs. By in-silico analysis, we have designed a peptide marker from amino acids at position of 93-107 (S. scrofa) and 93-112 (B. taurus) from peptide preptin region that resulted in different pattern of mass spectrum between the two species. We have also identified two DNA markers that can be detected by PCR using two primers sets designed from the IGF2 transcript sequences, to examine the presence of porcine in the sample. Thus, this study provides new genetic predictive markers, derived from the IGF2 gene, to identify the source of meat for halal authentication.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Intracellular antioxidant activity of Muntingia calabura leaves methanolic
           extract

    • Authors: AULIA NUR RAHMAWATI, OKID PARAMA ASTIRIN, ARTINI PANGASTUTI
      Pages: 210 - 14
      Abstract: Rahmawati AN, Astirin OP, Pangastuti A. 2018. Intracellular antioxidant activity of Muntingia calabura leaves methanolic
      extract. Nusantara Bioscience 19: 210-214. Excessive reactive oxygen species (ROS) results in oxidative damage that destroys proteins,
      lipids, and DNA, resulting in various diseases and accelerating the aging process. Antioxidants are compounds that have the ability to
      suppress oxidative stress. Muntingia calabura has a high phytochemical content, especially the phenolic group that can act as
      antioxidant. This study aimed to determine the intracellular antioxidant activity of M. calabura leaves methanolic extract (MLME)
      through intracellular ROS levels in 3T3 fibroblast cells under normal condition and oxidative stress due to UVB exposure. Tests were
      performed using the DPPH (Diphenyl-1-picrylhydrazyl) method and modified NBT (Nitroblue Tetrazolium). Testing of antioxidant
      activity with DPPH method obtained result that MLME had high antioxidant activity with IC50 value of 3.030 μg mL-1, while
      intracellular antioxidant activity with modified NBT method showed that MLME statistically had significant effect on intracellular ROS
      level under normal condition (p < 0.05) based on dose-dependent manner, but not significant (p > 0.05) under oxidative stress condition.
      Significant effects were only shown at a concentration of 20 μg mL-1 against the control of oxidative stress due to UVB exposure.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Effects of salinity and external proline on seed germination and early
           growth of Cucumis sativus cv. super ps

    • Authors: FARIBA AMINI, MEHRY ASKARY, FAHIMEH MOHTASHAMI, FAHIMEH MOHTASHAMI
      Pages: 215 - 220
      Abstract: Amini F, Askary M, Mohtashami F. 2018. Effects of salinity and external proline on seed germination and early growth of Cucumis sativus cv. super ps. Nusantara Bioscience 10: 215-220. Salinity is one of the important abiotic stresses that has an adverse effect on plant productions. High salt concentrations had negative effects on plant processes such as seed germination, seedling growth and turgescent, vegetative growth, flowering and fruiting stages. Proline as a compatible solution plays an important role in defense mechanisms of cellular stress. This study was done to evaluate effects of proline pretreatment (0 and 20 mM) in terms of salinity (0, 50, 100 and 150 mM) on cucumber cv. super ps. The obtained results proved that salinity treatments had significant effects on seed germination rate. On the contrary, application of proline had no significant effect on germination percentages of control and salt-stressed seeds. The root fresh weight and length of plant reduced with salinity increment while proline treatment had positive significant effects on the root dry as well as the fresh weight. Although, no significant variation was found in the root length and dry weight of cultivars treated with proline under salinity stresses. Meanwhile, the shoot length and dry weight of plants declined after salt-stressed treatments. While this condition increased the fresh weight of shoot. Proline treatments increased all of observed parameters but there were no significant variation found in the root length or dry weight of plants treated under saline stresses. The total soluble carbohydrate of plants had positive significant correlation with salt concentration, but the application of proline declined carbohydrate amounts in both salt-stressed and non-stressed plant. Salinity stress increased sodium, proline, protein contents and CAT enzyme, GPOX as well as MDA concentrations, while in the proline-treated plants salinity stresses had no significant effect on CAT, GPOX, MDA concentrations and internal proline amount.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Short Communication: Association of GH, IGF1R, and PIT1 genes polymorphism
           with average daily gain and body measurement in Pesisir cattle

    • Authors: ARNIM ., TINDA AFRIANI, YURNALIS ., DINO EKA PUTRA
      Pages: 221 - 225
      Abstract: Arnim, Yurnalis, Afriani T, Putra DE. 2017. Short Communication: Association of GH, IGF1R, and PIT1 genes polymorphism
      with average daily gain and body measurement in Pesisir cattle. Nusantara Bioscience 10: 221-225. Growth hormone (GH) and
      insuLin-like growth factor I receptor (IGF1R), and Pit1 gene has important effect on growth, carcass, and meat quality traits in many
      species. The objectives of the present study were to study the associations of the GH/AluI, GH/MboII, IGF1R/MspI and PIT1/HinfI with
      growth and body measurent such as body length (BL), chest girth (CG), height withers (HW) in Pesisir cattle. A total 175 Pesisir cattle
      were used in this study. Weight gain, and body measurement were fitted using the General Linear Model (GLM) procedure of the SAS
      program. The resuLts showed significant associations between the CC genotype of the GH/MboII polymorphism and higher weight gain
      and height withers (p < 0.05). The GH/AluI polymorphism did not have any effect on the weight gain and body measurement. Allele
      frequencies were found as A: 0.374 and B: 0.626 in GH/MboII polymorphism in GH gene. The effect of GH/AluI polymorphisms was
      not observed on average daily gain and body measurement in Pesisir cattle (P>0.05). The effect of IGF1R/MSpI polymorphism was not
      observed on average daily gain but show significant association between AA and AB genotype and height withers (p < 0.005). The
      PIT1/HinfI polymorphism was not associated with average daily gain and body measurement in Pesisir cattle. The resuLts demostrated
      that the GH/MboII and GHR1/MspI polymorphisms couLd be used as a candidate gene for selection in Pesisir cattle.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Effect of nodes position on the growth and yield of stem cutting of
           Sambiloto (Andrographis paniculata)

    • Authors: SOLIKIN SOLIKIN
      Pages: 226 - 231
      Abstract: Solikin. 2018. Effect of nodes position on the growth and yield of stem cutting of Sambiloto (Andrographis paniculata). Nusantara Bioscience 10: 226-231. Plant propagation by cuttings can be done to produce relatively uniform quality of plant yield such as sambiloto (Andrographis paniculata (Burm.f.) ex Nees). The study aimed to determine the effect of nodes position on the growth and yield of stem cutting of sambiloto. The experiment was conducted in Purwodadi Botanic Garden in February-May 2017. The study used randomized block design with the treatment of nodes potition i.e. top, middle and base of stem cuttings. Each treatment was replicated three times. The results showed that the top stem cutting produced the highest cutting life, leaf area, leaf dry weight, root and generative organs dry weight. In contrast, the base stem cutting produced the lowest live cutting and plant growth.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • The phytochemistry of Cymbopogon winterianus essential oil from Lombok
           Island, Indonesia and its antifungal activity against phytopathogenic
           fungi

    • Authors: PUTRI SRI ANDILA, I PUTU AGUS HENDRA, PUTRI KESUMA WARDANI, I GEDE TIRTA, SUTOMO SUTOMO, DEDEN FARDENAN
      Pages: 232 - 239
      Abstract: Andila PS, Hendra IPA, Wardani PK, Tirta IG, Sutomo, Fardenan D. 2018. The phytochemistry of Cymbopogon winterianus essential oil from Lombok Island, Indonesia and its antifungal activity against phytopathogenic fungi. Nusantara Bioscience 10: 232-239. Cymbopogon winterianus Jowitt ex Bor [Sereh Wangi] is a plant producing essential oil that has been extensively used in perfumery, mosquito repellent, medicinal, and cosmetic industry. In Indonesia, the accession of C. winterianus plant had been collected from natural forest in Sembalun, Lombok, West Nusa Tenggara (NTB) Province. However, the phytochemical properties and its biological potential of this accession have never been reported. The aim of this study was to determine the phytochemical content of essential oil of C. winterianus from Lombok and to explore its potential as a bioantifungal against several phytopathogen fungi. The essential oils were extracted from fresh stem and leaf of C. winterianus by hydrodistillation. The chemical composition of them was determined by chromatography-mass spectrometry (GC-MS). A total of 26 and 20 compounds were obtained from the stem and leaf oil with the dominant compounds of essential oil in stem were Torreyol (17.89%), Oxirane, octyl- (15.84%), (-)-Isopulegol (14.44%), Isopulegol 2 (12.18%), and Geranyl Acetate (5.89%) while in leaf were (-)-Isopulegol (22.69%), Oxirane, octyl- (16.64%), Cyclohexanol, 5-methyl-2-(1-methylethenyl)- (14.21%), "KW3 AUS Epiglobulol” (9.47%), and Cyclohexene, 1-methyl-4-(1-methylethenyl)- (7.14%). The presence of these contents as dominant chemical compounds in S. winterianus was reported for the first time. The highest content of oils was identified as monoterpene (38.01% and 45.78% in stem and leaf oil) and sesquiterpene ( 27.67% and 17.78 % in stem and Leaf oil). The biological study showed that leaf essential of C. winterianus expressed positive antifungal activity against Fusarium solani, Aspergillus niger, dan Cladosporium sp.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Production of bioethanol from sago hampas via Simultaneous
           Saccharification and Fermentation (SSF)

    • Authors: HUANG CHAI HUNG, DAYANG SALWANI AWANG ADENI, QUEENTETY JOHNNY, MICKY VINCENT
      Pages: 240 - 245
      Abstract: Huang CH, Adeni DSA, Johnny Q, Vincent M. 2018. Production of bioethanol from sago hampas via Simultaneous Saccharification and Fermentation (SSF). Nusantara Bioscience 10: 240-245. Sago hampas is an inexpensive, renewable and abundant agro-industrial residue that can be exploited to produce bioethanol. In this study, ethanol production was performed via simultaneous saccharification and fermentation (SSF) on fresh sago hampas at 2.5%, 5.0% and 7.5% (w/v) feedstock loadings with the aid of amylolytic enzymes, cellulolytic enzymes and Saccharomyces cerevisiae, under anaerobic condition for five days with a constant agitation of 150 rpm and ambient temperature. Results obtained indicated that SSF with 5.0% (w/v) sago hampas loading produced the highest ethanol yield at 17.79 g/L (79.65% Theoretical Ethanol Yield, TEY), while SSF using 2.5% and 7.5% (w/v) sago hampas produced ethanol at only 8.38 g/L (75.00% TEY) and 23.28 g/L (69.48% TEY), respectively. Total biomass reduction was recorded between 66.3% to 71.3% by the end of the SSF period. This study demonstrated that fresh sago hampas is a promising feedstock for bioethanol production as yields are generally high for all the substrate loadings tested. Moreover, bioethanol production using fresh sago hampas may assist in reducing pollution caused by sago waste accumulation.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • The role of neem leaves as organic pesticides in chili pepper (Capsicum
           frutescens)

    • Authors: SOPIALENA SOPIALENA, SURYA SILA, ROSFIANSYAH ROSFIANSYAH, JULI NURDIANA
      Pages: 246 - 250
      Abstract: Sopialena, Sila S, Rosfiansyah, Nurdiana J. 2018. The role of neem leaves as organic pesticides in chili pepper (Capsicum frutescens). Nusantara Bioscience 10: 246-250. The agricultural crops and horticultural plants are always under constant assault caused by diseases, insect pests, viruses, and other pathogens which may substantially reduce yield. Chili Pepper (Capsicum annum L.) is a popular horticultural plant of the Solanaceae family in Indonesia. Some serious diseases widely found in chili peppers, are anthracnose (Colletotrichum capsici), leaf spot (Cercospora capsici) and fruit rot (Phytophthora capsici). To manage the potential problems, this study is aimed to provide an explanatory knowledge of the use of plant-based pesticide to control the diseases in chili peppers. The information is meant to fill the knowledge gaps in the use of plant-based pesticide to control the chili diseases. The use of nonchemical pesticide benefits not only the environment but also as an organic strategy for disease management. This research used a randomized complete block design (RCBD) categorized into four groups and six different treatments. The organic pesticides were prepared from the extract of neem leaves, soursop leaves, lemongrass extract, tuba root extract, and kenikir/Cosmos caudatus extract). The result indicates that neem leaves are the most effective organic pesticides to control the chili pepper disease in Indonesia.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
  • Production of antioxidant compounds from tissue culture of Artemisia annua

    • Authors: AMALIA INDAH PRIHANTINI, AGUS SUKITO, SANRO TACHIBANA
      Pages: 251 - 255
      Abstract: Prihantini AI, Sukito A, Tachibana S. 2018. Production of antioxidant compound from tissue culture of Artemisia annua. Nusantara Bioscience 10: 251-255. Phenolic compounds have attracted much attention because of their role in some oxidative diseases. Therefore, the study aimed to investigate the production of antioxidant compound from tissue culture of A. annua. The callus were developed on solid MS medium treated with four combination of plant growth regulators: NAA 0.5 mg/L + BA 0.5 mg/L; NAA 0.5 mg/L + kinetin 0.5 mg/L; 2,4-D 0.5 mg/L + BA 0.5 mg/L; and 2,4-D 0.5 mg/L+ kinetin 0.5 mg/L. The callus extracts were evaluated their antioxidant activity and were analyzed the content of phenolic content. Furthermore, detection and quantification of phenolic compound were investigated. Combination of NAA 0.5 mg/L + BA 0.5 mg/L showed the highest DPPH radical scavenging activity and total phenolic content. GCMS analysis also revealed that the combination also showed the highest content of caffeic acid, a phenolic compound known as an antioxidant agent. Furthermore, the callus extract treated with NAA 0.5 mg/L + BA 0.5 mg/L increased both the antioxidant activity and caffeic acid content compared with A. annua leaf extract. In conclusion, plant tissue culture enhanced pharmaceutical properties of A. annua. Moreover, the study suggested that the treated callus extract of A. annua is potential to be further developed for production of antioxidant compound.
      PubDate: 2018-09-24
      Issue No: Vol. 10, No. 4 (2018)
       
 
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