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  Subjects -> HISTORY (Total: 1281 journals)
    - HISTORY (805 journals)
    - History (General) (51 journals)
    - HISTORY OF AFRICA (48 journals)
    - HISTORY OF ASIA (54 journals)
    - HISTORY OF AUSTRALASIA AREAS (7 journals)
    - HISTORY OF EUROPE (165 journals)
    - HISTORY OF THE AMERICAS (127 journals)
    - HISTORY OF THE NEAR EAST (24 journals)

HISTORY (805 journals)                  1 2 3 4 5 | Last

Showing 1 - 200 of 452 Journals sorted alphabetically
40 [degrees] South     Full-text available via subscription   (Followers: 2)
Aboriginal History     Full-text available via subscription   (Followers: 6)
Abstracta Iranica     Open Access   (Followers: 13)
Acadiensis : Journal of the History of the Atlantic Region / Acadiensis : revue d'histoire de la region Atlantique     Full-text available via subscription   (Followers: 5)
Accounting History Review     Hybrid Journal   (Followers: 17)
Acta Amazonica     Open Access   (Followers: 3)
Acta Historiae Artium     Full-text available via subscription   (Followers: 4)
Acta Orientalia     Full-text available via subscription   (Followers: 16)
Actes d'Història de la Ciència i de la Tècnica     Open Access  
Advances in Historical Studies     Open Access   (Followers: 3)
Advances in Software Engineering     Open Access   (Followers: 10)
Africa Confidential     Hybrid Journal   (Followers: 19)
Africa Research Bulletin: Political, Social and Cultural Series     Hybrid Journal   (Followers: 9)
Africa Today     Full-text available via subscription   (Followers: 16)
African and Asian Studies     Hybrid Journal   (Followers: 19)
African Diaspora     Open Access   (Followers: 8)
African Historical Review     Hybrid Journal   (Followers: 16)
African Natural History     Open Access   (Followers: 1)
Agora     Full-text available via subscription   (Followers: 3)
Agricultural History Review     Full-text available via subscription   (Followers: 9)
Akroterion     Full-text available via subscription   (Followers: 4)
Al-Masaq: Islam and the Medieval Mediterranean     Hybrid Journal   (Followers: 27)
albuquerque : revista de história     Open Access  
Almagest     Full-text available via subscription   (Followers: 1)
Altorientalische Forschungen     Hybrid Journal   (Followers: 6)
American Archivist     Hybrid Journal   (Followers: 156)
American Communist History     Hybrid Journal   (Followers: 18)
American Jewish History     Full-text available via subscription   (Followers: 16)
American Nineteenth Century History     Hybrid Journal   (Followers: 21)
American Periodicals : A Journal of History, Criticism, and Bibliography     Full-text available via subscription   (Followers: 7)
American Review of Canadian Studies     Hybrid Journal   (Followers: 6)
American Studies     Full-text available via subscription   (Followers: 21)
Amérique Latine Histoire et Mémoire     Open Access   (Followers: 2)
Amsterdamer Beitrage zur alteren Germanistik     Full-text available via subscription   (Followers: 3)
Amsterdamer Beitrage zur neueren Germanistik     Full-text available via subscription   (Followers: 3)
Anais do Museu Paulista : História e Cultura Material     Open Access   (Followers: 1)
Analecta Bollandiana     Full-text available via subscription   (Followers: 2)
Anales de Historia del Arte     Open Access   (Followers: 6)
Anatolica     Full-text available via subscription   (Followers: 10)
Ancient Civilizations from Scythia to Siberia     Hybrid Journal   (Followers: 10)
Ancient History : Resources for Teachers     Full-text available via subscription   (Followers: 1)
Anglican Historical Society Journal     Full-text available via subscription   (Followers: 2)
Annales historiques de la Révolution française     Open Access   (Followers: 11)
Annales UMCS, Historia     Open Access   (Followers: 5)
Annali dell'Istituto e Museo di storia della scienza di Firenze     Hybrid Journal  
Annuaire de l'Ecole pratique des hautes etudes. Section des sciences historiques et philologiques     Open Access   (Followers: 9)
Annual Bulletin of Historical Literature     Hybrid Journal   (Followers: 12)
ANQ: A Quarterly Journal of Short Articles, Notes, and Reviews     Hybrid Journal   (Followers: 1)
Antike und Abendland     Hybrid Journal   (Followers: 10)
Antiteses     Open Access  
Anuario de Estudios Atlánticos     Open Access   (Followers: 1)
Anuario de Historia de la Iglesia     Open Access   (Followers: 3)
Arabian Humanities     Open Access   (Followers: 1)
Arabica     Hybrid Journal   (Followers: 8)
ARAM Periodical     Full-text available via subscription   (Followers: 3)
Araucaria. Revista Iberoamericana de Filosofía, Política y Humanidades     Open Access   (Followers: 1)
Archeion     Full-text available via subscription  
ArcheoArte. Rivista Elettronica di Archeologia e Arte     Open Access   (Followers: 7)
Architectural Heritage     Hybrid Journal   (Followers: 24)
Archive for History of Exact Sciences     Hybrid Journal   (Followers: 7)
Archives Internationales d'Histoire des Sciences     Partially Free   (Followers: 2)
Archives of Natural History     Hybrid Journal   (Followers: 7)
Area     Hybrid Journal   (Followers: 12)
Arenal. Revista de historia de las mujeres     Open Access  
Aristotelian Society Supplementary Volume     Hybrid Journal   (Followers: 2)
Arizona Journal of Hispanic Cultural Studies     Full-text available via subscription   (Followers: 3)
Art History     Hybrid Journal   (Followers: 205)
Arthuriana     Full-text available via subscription   (Followers: 5)
Arys: Antigüedad, Religiones y Sociedades     Open Access   (Followers: 1)
Aschkenas     Hybrid Journal   (Followers: 3)
Asia Pacific Business Review     Hybrid Journal   (Followers: 5)
Asia Pacific Journal of Education     Hybrid Journal   (Followers: 18)
Asia-Pacific Journal : Japan Focus     Open Access   (Followers: 7)
Asian Journal of Social Psychology     Hybrid Journal   (Followers: 5)
Asian Perspectives     Full-text available via subscription   (Followers: 9)
Asian Philosophy: An International Journal of the Philosophical Traditions of the East     Hybrid Journal   (Followers: 7)
Asian Studies Review     Hybrid Journal   (Followers: 17)
Aspasia     Full-text available via subscription  
Astérion     Open Access   (Followers: 1)
Ateliê de História UEPG     Open Access  
Aurora Journal     Full-text available via subscription  
Austral Ecology     Hybrid Journal   (Followers: 12)
Australasian Journal of Irish Studies, The     Full-text available via subscription   (Followers: 9)
Australasian Review of African Studies, The     Full-text available via subscription   (Followers: 2)
Australian Antarctic Magazine     Free   (Followers: 4)
Australian Cultural History     Hybrid Journal   (Followers: 11)
Australian Historical Studies     Hybrid Journal   (Followers: 13)
Australian Journal of Legal History     Full-text available via subscription   (Followers: 19)
Australian Journal of Politics & History     Hybrid Journal   (Followers: 13)
Balkanologie : Revue d'Études Pluridisciplinaires     Open Access   (Followers: 4)
Baltic-Pontic Studies     Open Access   (Followers: 1)
Behemoth     Open Access   (Followers: 3)
Berichte Zur Wissenschaftsgeschichte     Hybrid Journal   (Followers: 9)
BIBLOS - Revista do Departamento de Biblioteconomia e História     Open Access   (Followers: 13)
Biodiversity and Natural History     Open Access   (Followers: 5)
Biographical Memoirs of Fellows of the Royal Society     Full-text available via subscription   (Followers: 2)
Biography     Full-text available via subscription   (Followers: 18)
Bochumer Philosophisches Jahrbuch für Antike und Mittelalter     Hybrid Journal   (Followers: 2)
Body & Society     Hybrid Journal   (Followers: 21)
Boletim Cearense de Educação e História da Matemática     Open Access  
Book History     Full-text available via subscription   (Followers: 116)
Boom : A Journal of California     Full-text available via subscription   (Followers: 1)
Britain and the World     Hybrid Journal   (Followers: 9)
British Journal for Military History     Open Access   (Followers: 23)
British Journal of Canadian Studies     Hybrid Journal   (Followers: 11)
British Journal of Middle Eastern Studies     Hybrid Journal   (Followers: 24)
British Mycological Society Symposia Series     Full-text available via subscription   (Followers: 1)
British Review of New Zealand Studies     Full-text available via subscription   (Followers: 2)
Bryn Mawr Classical Review     Open Access   (Followers: 32)
BSHM Bulletin: Journal of the British Society for the History of Mathematics     Hybrid Journal   (Followers: 6)
Buildings & Landscapes: Journal of the Vernacular Architecture Forum     Full-text available via subscription   (Followers: 13)
Bulletin d'histoire politique     Full-text available via subscription   (Followers: 3)
Bulletin de la Sabix     Open Access  
Bulletin de Philosophie Medievale     Full-text available via subscription   (Followers: 5)
Bulletin du centre d’études médiévales d’Auxerre     Open Access   (Followers: 6)
Bulletin d’études Orientales     Open Access   (Followers: 10)
Bulletin of Hispanic Studies     Hybrid Journal   (Followers: 11)
Bulletin of Latin American Research     Hybrid Journal   (Followers: 15)
Bulletin of Spanish Studies: Hispanic Studies and Researches on Spain, Portugal and Latin America     Hybrid Journal   (Followers: 23)
Bulletin of the Ecological Society of America     Open Access   (Followers: 4)
Bulletin of the John Rylands Library     Hybrid Journal   (Followers: 12)
Bustan     Hybrid Journal   (Followers: 1)
Byzantine and Modern Greek Studies     Hybrid Journal   (Followers: 10)
Byzantinische Zeitschrift     Hybrid Journal   (Followers: 11)
Byzantion Nea Hellás     Open Access   (Followers: 2)
C@hiers du CRHIDI     Open Access  
Cabo     Full-text available via subscription  
Cadernos de História     Open Access  
CADUS - Revista de Estudos de Política, História e Cultura     Open Access  
Cahiers d'histoire     Full-text available via subscription   (Followers: 2)
Cahiers d'histoire. Revue d'histoire critique     Open Access   (Followers: 12)
Cahiers de l'Urmis     Open Access   (Followers: 1)
Cahiers des études anciennes     Open Access   (Followers: 7)
Cahiers du Centre de recherches historiques     Open Access   (Followers: 9)
Cahiers du Monde Russe     Open Access   (Followers: 10)
Cahiers d’études africaines     Open Access   (Followers: 9)
Cahiers « Mondes anciens »     Open Access   (Followers: 5)
California Italian Studies Journal     Full-text available via subscription   (Followers: 5)
Cambridge Journal of Regions, Economy and Society     Hybrid Journal   (Followers: 9)
Canadian Historical Review     Full-text available via subscription   (Followers: 10)
Canadian Journal of History     Full-text available via subscription   (Followers: 8)
Canadian Journal of Linguistics / La revue canadienne de linguistique     Full-text available via subscription   (Followers: 8)
Canadian Review of American Studies     Full-text available via subscription   (Followers: 1)
Canadian-American Slavic Studies     Hybrid Journal   (Followers: 7)
Caribbean Studies     Full-text available via subscription   (Followers: 11)
Catholic Historical Review     Full-text available via subscription   (Followers: 15)
Cemoti, Cahiers d'études sur la méditerranée orientale et le monde turco-iranien     Open Access   (Followers: 2)
Central Asian Survey     Hybrid Journal   (Followers: 8)
Central Europe     Hybrid Journal   (Followers: 5)
Chaucer Review     Full-text available via subscription   (Followers: 7)
Childhood in the Past : An International Journal     Hybrid Journal   (Followers: 3)
Chinese Studies in History     Full-text available via subscription   (Followers: 9)
Chronica Nova. Revista de Historia Moderna de la Universidad de Granada     Open Access  
Chronique d'Egypte     Full-text available via subscription   (Followers: 6)
Church History and Religious Culture     Hybrid Journal   (Followers: 53)
Church History: Studies in Christianity and Culture     Full-text available via subscription   (Followers: 73)
Civil War History     Full-text available via subscription   (Followers: 19)
Cleveland Studies in the History of Art     Full-text available via subscription   (Followers: 2)
CLIO : Revista de Pesquisa Histórica     Open Access  
Clio y Asociados     Open Access  
Clio. Femmes, Genre, Histoire - Articles     Open Access   (Followers: 11)
Cliodynamics     Open Access  
Collections électroniques de l'INHA     Open Access   (Followers: 1)
Collingwood and British Idealism Studies     Full-text available via subscription   (Followers: 1)
Colonial Latin American Review     Hybrid Journal   (Followers: 9)
Comitatus : A Journal of Medieval and Renaissance Studies     Full-text available via subscription   (Followers: 16)
Comparative Legal History     Full-text available via subscription   (Followers: 5)
Comparative Studies of South Asia, Africa and the Middle East     Full-text available via subscription   (Followers: 18)
Comptabilités     Open Access  
Concorso. Arti e lettere     Open Access  
Conservative Judaism     Full-text available via subscription   (Followers: 5)
Conserveries mémorielles     Open Access  
Contemporaneity : Historical Presence in Visual Culture     Open Access   (Followers: 3)
Contemporary Arab Affairs     Hybrid Journal   (Followers: 3)
Contemporary British History     Hybrid Journal   (Followers: 21)
Contemporary French and Francophone Studies     Hybrid Journal   (Followers: 10)
Convivium     Full-text available via subscription  
Crime, Histoire & Sociétés     Open Access   (Followers: 3)
Critical Historical Studies     Full-text available via subscription   (Followers: 6)
Critique: Journal of Socialist Theory     Hybrid Journal   (Followers: 5)
Cromohs : Cyber Review of Modern Historiography     Open Access  
Crossing Borders : An Interdisciplinary Journal of Undergraduate Scholarship     Open Access   (Followers: 1)
Cuadernos de Historia     Open Access   (Followers: 3)
Cuadernos de historia de España     Open Access   (Followers: 3)
Cuadernos de Historia de la Salud Publica     Open Access  
Cuadernos de Historia del Derecho     Open Access   (Followers: 5)
Cuadernos de Historia Moderna     Open Access   (Followers: 2)
Cuadernos de Ilustración y Romanticismo     Open Access  
Cuban Studies     Full-text available via subscription   (Followers: 6)
Cuicuilco     Open Access   (Followers: 1)
Cultura Histórica & Patrimônio     Open Access  
Cultural and Social History     Full-text available via subscription   (Followers: 39)
Cultural History     Hybrid Journal   (Followers: 19)
Cultural-Historical Psychology     Open Access  
Culturas     Open Access   (Followers: 1)
Cultures & conflits     Open Access   (Followers: 10)
Cultures et conflits     Open Access   (Followers: 1)
Czech-Polish Historical and Pedagogical Journal     Open Access  
Dapim : Studies on the Holocaust     Hybrid Journal   (Followers: 8)
Das Mittelalter     Hybrid Journal   (Followers: 7)

        1 2 3 4 5 | Last

Journal Cover Bulletin of the Ecological Society of America
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 0012-9623
   Published by John Wiley and Sons Homepage  [1583 journals]
  • The Cytotoxic Effects of Camptothecin and Mastoparan on the Unicellular
           Green Alga Chlamydomonas reinhardtii

    • Authors: Jürgen Voigt; Markus Morawski, Johannes Wöstemeyer
      Abstract: We have recently reported that protease inhibitors affecting the activity of the proteasome cause necrotic cell death in Chlamydomonas reinhardtii instead of inducing apoptosis as shown for some mammalian cell lines. Therefore, we have studied other well-known inducers of apoptosis in mammalian cells for their effects on C. reinhardtii cells. Mastoparan caused rapid cell death without a prominent lag-phase under all growth conditions, whereas the cytotoxic effect of the topoisomerase I inhibitor camptothecin exclusively occurred during the cell-division phase. Essentially no differences between wall-deficient and wild-type cells were observed with respect to dose-response and time-course of camptothecin and mastoparan. In cultures of the wall-deficient strain, cell death was accompanied by swelling and subsequent disruption of the cells, established markers of necrosis. In case of the wild-type strain, camptothecin and mastoparan caused accumulation of apparently intact, but dead cells instead of cell debris due to the presence of the wall. Both in cultures of the wall-deficient and the wild-type strains, cell death was accompanied by an increase of the protein concentration in the culture medium indicating a lytic process like necrosis. Taking together, we have severe doubts on the existence of an apoptotic program in case of C. reinhardtii.
      PubDate: 2017-04-20T00:45:57.7052-05:00
      DOI: 10.1111/jeu.12413
       
  • Lineage-Specific and Highly Derived Gene Sequences Among Amoebozoa,
           Revealed by the Comparative Analysis of Transcriptomes from Twelve
           Amoebozoan Species

    • Authors: Natalya I. Bondarenko; Anton S. Bondarenko, Alexey V. Smirnov
      Abstract: Amoebozoa represent a difficult group for traditional morphology-based taxonomy. Molecular approaches, such as gene sequencing and DNA barcoding have greatly enhanced our knowledge of the diversity of these organisms. However, metagenomic studies of Amoebozoa still did not provide as impressive results as they did among some other groups of protists. In environmental DNA surveys done on fragments of SSU rDNA gene and other traditional DNA barcodes, Amoebozoa genes normally constitute a minor part of the total gene diversity and represent only the most abundant lineages. A potential way to resolve this problem is the usage of DNA barcodes based on genes, which are unique or highly derived in this group of organisms. In the present study, we attempted to find such genes and gene families with a low level of paralogy, potentially appropriate as Amoebozoa-specific DNA barcodes. For this we re-assembled transcriptomes of 12 amoebozoan species available from the public databases and performed gene annotation and identification of orthologous genes. In our analysis Amoebozoa-specific and highly derived sequences formed 53,182 clusters of orthologs, containing from 2 to 299 proteins each. Some of these genes may be a potential target for DNA barcoding of Amoebozoa.
      PubDate: 2017-04-10T02:19:13.520267-05:
      DOI: 10.1111/jeu.12397
       
  • Morphology and Phylogeny of the Soil Ciliate Metopus yantaiensis n. sp.
           

    • Authors: Atef Omar; Qianqian Zhang, Songbao Zou, Jun Gong
      Abstract: The morphology and infraciliature of a new ciliate, Metopus yantaiensis n. sp., discovered in coastal soil of northern China, were investigated. It is distinguished from its congeners by a combination of the following features: nuclear apparatus situated in the preoral dome; 18–21 somatic ciliary rows, of which three extend onto the preoral dome (dome kineties); three to five distinctly elongated caudal cilia, and 21–29 adoral polykinetids. The 18S rRNA genes of this new species and two congeners, Metopus contortus and Metopus hasei, were sequenced and phylogenetically analyzed. The new species is more closely related to M. hasei and the clevelandellids than to other congeners; both the genus Metopus and the order Metopida are not monophyletic. In addition, the digestion-resistant bacteria in the cytoplasm of M. yantaiensis were identified, using a 16S rRNA gene clone library, sequencing, and fluorescence in situ hybridization. The detected intracellular bacteria are affiliated with Sphingomonadales, Rhizobiales, Rickettsiales (Alphaproteobacteria), Pseudomonas (Gammaproteobacteria), Rhodocyclales (Betaproteobacteria), Clostridiales (Firmicutes), and Flavobacteriales (Bacteroidetes).
      PubDate: 2017-04-10T02:16:53.769892-05:
      DOI: 10.1111/jeu.12411
       
  • A Novel Spore Wall Protein from Antonospora locustae (Microsporidia:
           Nosematidae) Contributes to Sporulation

    • Authors: Longxin Chen; Runting Li, Yinwei You, Kun Zhang, Long Zhang
      Abstract: Microsporidia are obligate intracellular parasites, existing in a wide variety of animal hosts. Here, we reported AlocSWP2, a novel protein identified from the spore wall of Antonospora locustae (formerly, Nosema locustae, and synonym, Paranosema locustae), containing four cysteines that are conserved among the homologues of several Microspodian pathogens in insects and mammals. AlocSWP2 was detected in the wall of mature spores via indirect immunofluorescence assay. In addition, immunocytochemistry localization experiments showed that the protein was observed in the wall of sporoblasts, sporonts, and meronts during sporulation within the host body, also in the wall of mature spores. AlocSWP2 was not detected in the fat body of infected locust until the 9th day after inoculating spores via RT-PCR experiments. Furthermore, the survival percentage of infected locusts injected with dsRNA of AlocSWP2 on the 15th, 16th, and 17th days after inoculation with microsporidian were significantly higher than those of infected locusts without dsRNA treatment. Conversely, the amount of spores in locusts infected with A. locustae after treated with RNAi AlocSWP2 was significantly lower than those of infected locusts without RNAi of this gene. This novel spore wall protein from A. locustae may be involved in sporulation, thus contributing to host mortality.
      PubDate: 2017-04-10T02:06:04.449811-05:
      DOI: 10.1111/jeu.12410
       
  • Use of Highly Specific Molecular Markers Reveals Positive Correlation
           between Abundances of Mesodinium cf. major and Its Preferred Prey,
           Teleaulax amphioxeia, During Red Water Blooms in the Columbia River
           Estuary

    • Authors: Lydie Herfort; Katie Maxey, Ian Voorhees, Holly M. Simon, Kolette Grobler, Tawnya D. Peterson, Peter Zuber
      Abstract: In a previous study, Teleaulax amphioxeia—the preferred prey of Mesodinium in the Columbia River estuary—were undetectable within intense annual blooms, suggesting blooms are prey-limited or prey are acquired outside of bloom patches. We used a novel molecular approach specifically targeting the prey (i.e., Unique Sequence Element [USE] within the ribosomal RNA 28S D2 regions of T. amphioxeia nucleus and nucleomorph) in estuarine water samples acquired autonomously with an Environmental Sample Processor integrated within a monitoring network (ESP-SATURN). This new approach allowed for both more specific detection of the prey and better constraint of sample variability. A positive correlation was observed between abundances of M. cf. major and T. amphioxeia during bloom periods. The correlation was stronger at depth (> 8.2 m) and weak or nonexistent in the surface, suggesting that predator–prey dynamics become uncoupled when stratification is strong. We confirmed exclusive selectivity for T. amphioxeia by M. cf. major and observed the incorporation of the prey nucleus into a 4-nuclei complex, where it remained functionally active. The specific biomarker for T. amphioxeia was also recovered in M. cf. major samples from a Namibian coastal bloom, suggesting that a specific predator–prey relationship might be widespread between M. cf. major and T. amphioxeia.
      PubDate: 2017-04-07T04:37:21.874693-05:
      DOI: 10.1111/jeu.12407
       
  • A New Heterolobosean Amoeboflagellate, Tetramitus dokdoensis n. sp.,
           Isolated from a Freshwater Pond on Dokdo Island in the East Sea, Korea

    • Authors: Jong Soo Park
      Abstract: The genus Tetramitus is a representative amoeboflagellate group within the Heterolobosea, and currently contains over a dozen species. Here, a new heterolobosean amoeboflagellate was isolated from a freshwater pond on Dokdo Island, Korea. The amoebae have eruptive pseudopodia, no uroidal filament, and a nucleus with a central nucleolus. The length and width of the amoebae are 15.5–28.0 μm and 5.4–12.6 μm, respectively. The flagellates are conical, with 4 flagella of equal length (~10 μm). There is a discrete rostrum in the subapical region of the flagellate form. The cyst has thin endo- and ectocyst layers and no cyst pores. The amoeba shows slow movement at 37 °C, but does not move at 42 °C under a light microscope. Phylogenies of the 18S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 sequence show that the strain belongs to a subclade of Tetramitus that includes Tetramitus rostratus, Tetramitus waccamawensis and Tetramitus entericus, amongst others. Nonetheless, the strain is distinct from other species in both molecular phylogenetic trees. Thus the strain isolated from the Dokdo Island is proposed as a novel species, Tetramitus dokdoensis n. sp.
      PubDate: 2017-04-06T06:23:37.455395-05:
      DOI: 10.1111/jeu.12409
       
  • Differences in the Detection of BrdU/EdU Incorporation Assays Alter the
           

    • Authors: Marcelo Santos Silva; Paula Andrea Marin Muñoz, Hugo Aguirre Armelin, Maria Carolina Elias
      Abstract: Trypanosomatids are the etiologic agents of various infectious diseases in humans. They diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary and comparative studies. Here, we show a meticulous study comparing the incorporation and detection of the thymidine analogs BrdU and EdU in Leishmania amazonensis, Trypanosoma brucei, and Trypanosoma cruzi to monitor their DNA replication. We used BrdU- and EdU-incorporated parasites with the respective standard detection approaches: indirect immunofluorescence to detect BrdU after standard denaturation (2 M HCl) and “click” chemistry to detect EdU. We found a discrepancy between these two thymidine analogs due to the poor detection of BrdU, which is reflected on the estimative of the duration of the cell cycle phases G1, S, and G2. To solve this discrepancy, we increase the exposure of incorporated BrdU using different concentrations of HCl. Using a new value for HCl concentration, we re-estimated the phases G1, S, G2 + M, and cytokinesis durations, confirming the values found by this approach using EdU. In conclusion, we suggest that the studies using BrdU with standard detection approach, not only in trypanosomatids but also in others cell types, should be reviewed to ensure an accurate estimation of DNA replication monitoring.
      PubDate: 2017-03-28T02:15:37.710913-05:
      DOI: 10.1111/jeu.12408
       
  • Genetic Determinism vs. Phenotypic Plasticity in Protist Morphology

    • Authors: Matthieu Mulot; Katarzyna Marcisz, Lara Grandgirard, Enrique Lara, Anush Kosakyan, Bjorn J. M. Robroek, Mariusz Lamentowicz, Richard J. Payne, Edward A. D. Mitchell
      Abstract: Untangling the relationships between morphology and phylogeny is key to building a reliable taxonomy, but is especially challenging for protists, where the existence of cryptic or pseudocryptic species makes finding relevant discriminant traits difficult. Here we use Hyalosphenia papilio (a testate amoeba) as a model species to investigate the contribution of phylogeny and phenotypic plasticity in its morphology. We study the response of H. papilio morphology (shape and pores number) to environmental variables in (i) a manipulative experiment with controlled conditions (water level), (ii) an observational study of a within-site natural ecological gradient (water level), and (iii) an observational study across 37 European peatlands (climate). We showed that H. papilio morphology is correlated to environmental conditions (climate and water depth) as well as geography, while no relationship between morphology and phylogeny was brought to light. The relative contribution of genetic inheritance and phenotypic plasticity in shaping morphology varies depending on the taxonomic group and the trait under consideration. Thus, our data call for a reassessment of taxonomy based on morphology alone. This clearly calls for a substantial increase in taxonomic research on these globally still under-studied organisms leading to a reassessment of estimates of global microbial eukaryotic diversity.
      PubDate: 2017-03-28T02:10:27.303066-05:
      DOI: 10.1111/jeu.12406
       
  • Role of Modular Polyketide Synthases in the Production of Polyether Ladder
           Compounds in Ciguatoxin-Producing Gambierdiscus polynesiensis and G.
           excentricus (Dinophyceae)

    • Authors: Gurjeet S. Kohli; Katrina Campbell, Uwe John, Kirsty F. Smith, Santiago Fraga, Lesley L. Rhodes, Shauna A. Murray
      Abstract: Gambierdiscus, a benthic dinoflagellate, produces ciguatoxins that cause the human illness Ciguatera. Ciguatoxins are polyether ladder compounds that have a polyketide origin, indicating that polyketide synthases (PKS) are involved in their production. We sequenced transcriptomes of Gambierdiscus excentricus and Gambierdiscus polynesiensis and found 264 contigs encoding single domain ketoacyl synthases (KS; G. excentricus: 106, G. polynesiensis: 143) and ketoreductases (KR; G. excentricus: 7, G. polynesiensis: 8) with sequence similarity to type I PKSs, as reported in other dinoflagellates. In addition, 24 contigs (G. excentricus: 3, G. polynesiensis: 21) encoding multiple PKS domains (forming typical type I PKSs modules) were found. The proposed structure produced by one of these megasynthases resembles a partial carbon backbone of a polyether ladder compound. Seventeen contigs encoding single domain KS, KR, s-malonyltransacylase, dehydratase and enoyl reductase with sequence similarity to type II fatty acid synthases (FAS) in plants were found. Type I PKS and type II FAS genes were distinguished based on the arrangement of domains on the contigs and their sequence similarity and phylogenetic clustering with known PKS/FAS genes in other organisms. This differentiation of PKS and FAS pathways in Gambierdiscus is important, as it will facilitate approaches to investigating toxin biosynthesis pathways in dinoflagellates.
      PubDate: 2017-03-23T10:20:28.183513-05:
      DOI: 10.1111/jeu.12405
       
  • Description and Molecular Phylogeny of a Novel Hypotrich Ciliate from the
           Soil of Marche Region, Italy; Including Notes on the MOSYSS Project

    • Authors: Daizy Bharti; Santosh Kumar, Antonietta La Terza
      Abstract: The morphology and morphogenesis during cell division of a new stylonychine hypotrich, Rigidocortex quadrinucleatus n. sp., were investigated using live observation and protargol staining. The new species was isolated from soil samples collected from an organic farm in the Marche Region, Italy, in framework of the MOSYSS project. Rigidocortex quadrinucleatus is characterized as follows: cell size about 180 × 80 μm in vivo; four ellipsoidal macronuclear nodules; 44 adoral membranelles: 18 fronto-ventral-transverse cirri consisting of three frontal, four frontoventral, one buccal, three ventral, two pretransverse, and five transverse cirri; dorsal kinety 3 with multiple fragmentation; resting cyst with hyaline ridges. Rigidocortex quadrinucleatus mainly differs from the type species R. octonucleatus in having four (vs. eight) macronuclear nodules. Rigidocortex quadrinucleatus can be easily confused with Sterkiella cavicola since both have a rather similar ventral ciliature; however, they can be separated by the slightly higher number of cirri in the left marginal row that runs along the posterior cell's margin in R. quadrinucleatus. Morphogenesis on the ventral surface is highly similar to that of Sterkiella species, but differs significantly on the dorsal surface (multiple vs. simple fragmentation of dorsal kinety 3). Phylogenetic analyses based on SSU rRNA gene sequences consistently place the new species within the stylonychine oxytrichids, clustering closer to Gastrostyla steinii than to S. cavicola.
      PubDate: 2017-03-19T21:11:03.850739-05:
      DOI: 10.1111/jeu.12404
       
  • Molecular Phylogeny and Ultrastructure of Aphelidium desmodesmi, a New
           Species in Aphelida (Opisthosporidia)

    • Authors: Peter M. Letcher; Martha J. Powell, Philip A. Lee, Salvador Lopez, Micheal Burnett
      Abstract: Aphelids are a diverse group of intracellular parasitoids of algae and diatoms, and are sister to true fungi. Included in four genera, the 14 described species utilize phagocytosis as their mode of nutrition, and the life cycles of these taxa are remarkably similar. However, their putative specificity of host, morphological and ultrastructural features, and genetic divergence have been considered in taxon delineation. Here, we examine the host specificity, morphology, ultrastructure, and molecular 18S gene sequence of a new species in Aphelida, Aphelidium desmodesmi sp. nov. This taxon is in a well-supported clade with two other species of Aphelidium, and this lineage is sister to Amoeboaphelidium and Paraphelidium. Of interest, the mitochondrial structure of Aph. desmodesmi is more like that of Paraphelidium than that of Aphelidium aff. melosirae, the only other species of Aphelidium to have been examined ultrastructurally. This research examines and expands our understanding of host range, morphological diversity, and genetic divergence of the aphelids.
      PubDate: 2017-03-10T10:49:07.129471-05:
      DOI: 10.1111/jeu.12401
       
  • Survey for Zoonotic Microsporidian Pathogens in Wild Living Urban Rooks
           (Corvus frugilegus)

    • Authors: Agnieszka Perec-Matysiak; Maria Wesołowska, Kinga Leśniańska, Katarzyna Buńkowska-Gawlik, Joanna Hildebrand, Marta Kicia
      Abstract: Microsporidia are opportunistic pathogens in nature infecting all animal phyla. There is a potential risk of microsporidian spores transmission from urban rooks inhabiting some metropolitan cities to people through casual interactions. The aim of this study was to identify microsporidia species in the droppings of rooks in Wroclaw, Poland. A total of 15 collective sets of droppings were examined using nested-PCR method. Amplification of ITS rRNA gene revealed the presence of Enterocytozoon bieneusi D, Peru 6, and Encephalitozoon hellem 1A genotypes. This study indicates that excreta of urban rooks can be an important source of human infection with these pathogens.
      PubDate: 2017-03-10T10:48:55.940816-05:
      DOI: 10.1111/jeu.12402
       
  • The Synonymy of the Toxic Dinoflagellates Prorocentrum mexicanum and
           P. rhathymum and the Description of P. steidingerae sp. nov.
           (Prorocentrales, Dinophyceae)

    • Authors: Fernando Gómez; Dajun Qiu, Senjie Lin
      Abstract: Prorocentrum mexicanum and P. rhathymum are toxicologically important dinoflagellates, but their relationship is not well defined. We investigated strains from Puerto Rico and Brazil by light and scanning electron microscopies. We provide molecular data from a strain isolated near the type locality of P. rhathymum, and the first morphological and molecular data from the South Atlantic Ocean. The rRNA gene (rDNA) sequences of the Puerto Rican and Brazilian strains were identical, and their morphologies fit the description of P. rhathymum. In the molecular phylogenies, the globally distributed populations under the names P. mexicanum and P. rhathymum are intermixed and branched together, except for several divergent strains from Florida and Cuba. We examined the original descriptions and iconotypes of the species Prorocentrum maximum, P. brochii, P. mexicanum, and P. rhathymum. We conclude that P. maximum sensu Schiller's figure 41a corresponds to the earlier description of this species; the split of P. mexicanum and P. rhathymum was based on a misidentification because P. mexicanum sensu Cortés-Altamirano & Sierra-Beltrán corresponds to P. texanum var. cuspidatum; and P. rhathymum is a junior synonym of P. mexicanum. Several Floridian and Cuban strains correspond to a new species, which we describe as Prorocentrum steidingerae sp. nov.
      PubDate: 2017-03-10T10:48:48.283806-05:
      DOI: 10.1111/jeu.12403
       
  • Differential Gene Expression in Five Isolates of the Clam Pathogen, Quahog
           Parasite Unknown (QPX)

    • Authors: Ewelina Rubin; Arnaud Tanguy, Emmanuelle Pales Espinosa, Bassem Allam
      Abstract: Quahog parasite unknown (QPX) is a thraustochytrid protist that infects the hard clam, Mercenaria mercenaria, causing significant economic losses along the northeastern coast of North America. Previous investigations noted differences in growth dynamics and virulence in QPX cells from different geographic locations. In order to probe the molecular determinants for these variations, we investigated the transcriptomic profiles of five geographically distinct QPX isolates using custom 15k 60-mer oligonucleotide arrays. A total of 1,263 transcripts were differentially expressed (DE) among the five QPX isolates. The hierarchical clustering of gene expression profiles showed that the QPX isolates from Raritan Bay (RB, NY) and from Provincetown Harbor (MA) were more similar to each other and diverged from QPX isolates from Peconic Bay (PB, NY) and Old Plantation Creek (VA), which had more similar gene expression profiles. The most prominent difference was based on 78 transcripts coding for heat shock proteins DE between the five QPX isolates. The study generated contrasting transcriptomic profiles for QPX isolated from northern (MA) and deeper (RB, NY) locations as compared to southern (VA) and shallower (PB, NY) areas, suggesting the adaptation of the parasite to local environmental, in particular temperature, conditions.
      PubDate: 2017-03-10T10:47:21.478566-05:
      DOI: 10.1111/jeu.12400
       
  • Occurrence and Genetic Characteristics of Cryptosporidium hominis and
           Cryptosporidium andersoni in Horses from Southwestern China

    • Authors: Lei Deng; Wei Li, Zhijun Zhong, Chao Gong, Xuefeng Cao, Yuan Song, Wuyou Wang, Xiangming Huang, Xuehan Liu, Yanchun Hu, Hualin Fu, Min He, Ya Wang, Yue Zhang, Kongju Wu, Guangneng Peng
      Abstract: A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptosporidium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes. Subtyping analysis of the 60-kDa glycoprotein (GP60) gene sequence of C. hominis revealed a new rare subtype Id, named IdA15; only three Id isolates have been reported in humans to date. Multilocus sequence typing (MLST) analysis indicated that the C. andersoni subtype was A6, A5, A2, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively). This is the first report to identify the presence of C. andersoni and C. hominis in horses in southwestern China and the first to identify a rare zoonotic subtype Id of C. hominis in horses. These findings suggest that infected horses may act as potential reservoirs of Cryptosporidium to transmit infections to humans.
      PubDate: 2017-03-08T13:15:26.243506-05:
      DOI: 10.1111/jeu.12399
       
  • Evaluation of Systematic Position of Helicoprorodontids and Chaeneids
           (Ciliophora, Litostomatea): An Attempt to Break Long Branches in 18S rRNA
           Gene Phylogenies

    • Authors: Peter Vďačný; Matej Rataj
      Abstract: Phylogenetic position of some free-living litostomatean taxa has not been correctly determined because of long-branch artifacts in 18S rRNA gene trees. The main aim of this study was to test the effectiveness of various masking algorithms, tree-building techniques, binarization of DNA data as well as combining morphological and molecular data to eliminate long-branch attraction of two problematic groups, helicoprorodontids and chaeneids. Guidance and SlowFaster masking in a combination with PhyloBayesian tree construction erased the artifactual positions of helicoprorodontids and chaeneids. On the other hand, binarization of DNA sequences and the strategy of combining morphological and molecular data eliminated only the artifactual position of chaeneids but not that of helicoprorodontids which were still being attracted by out-group taxa. According to statistical tree topology tests and comparative morphological studies, helicoprorodontids are classified as a distinct order while chaeneids are considered to be fast evolving members of the order Lacrymariida. The high body contractility, “cephalization” of the anterior body end, and helicalization of the anterior portion of some or all somatic ciliary rows indicate relatedness of helicoprorodontids, chaeneids, and lacrymariids. On the other hand, the dorsal brush separated from the circumoral kinety by dense ciliary files supports kinships of chaeneids, lacrymariids, and didiniids.
      PubDate: 2017-03-07T11:55:29.906867-05:
      DOI: 10.1111/jeu.12396
       
  • Morphology and Morphogenesis of a Novel Saline Soil Hypotrichous Ciliate,
           Gonostomum sinicum nov. spec. (Ciliophora, Hypotrichia, Gonostomatidae),
           Including a Report on the Small Subunit rDNA Sequence

    • Authors: Xiaoteng Lu; Jie Huang, Chen Shao, Saleh A. Al-Farraj, Shan Gao
      Abstract: Morphology, cirral pattern, and morphogenesis of the new saline soil hypotrich, Gonostomum sinicum nov. spec. collected from Longfeng Wetland in Daqing, north China, were studied, using detailed live observations and protargol-stained specimens. The new species is characterized as follows: (i) a size in vivo of 100–125 × 30–40 μm, (ii) colorless cortical granules, 0.5 μm across, arranged in short rows, (iii) an adoral zone composed of 28–33 membranelles, (iv) three or four frontoventral rows, one of which extends onto the postoral area, (v) left and right marginal rows composed of 18–27 and 21–35, cirri, respectively, and (vi) usually two transverse cirri. Morphogenesis is as usual for the genus Gonostomum, i.e. the cirral primordia II–VI are primary primordia which split into two sets for proter and opisthe in division middle stages, except for anlage I which develops independently. However, the number of frontoventral transverse anlagen is either five or six not only in different individuals but even in proter and opisthe of the same divider. The phylogenetic analyses based on SSU rDNA sequences showed that the genus Gonostomum is nonmonophyletic, indicating that the patterns of cirri and dorsal kineties are homoplasious characters. The new species G. sinicum nov. spec. is perhaps closely related to Cotterillia bromelicola and two congeners.
      PubDate: 2017-02-28T09:25:40.184369-05:
      DOI: 10.1111/jeu.12398
       
  • In Memoriam: Norman E. Williams (1928–2016): Pioneer of Ciliate
           Architecture

    • Authors: Joseph Frankel; Howard E. Buhse
      PubDate: 2017-02-28T09:25:27.725113-05:
      DOI: 10.1111/jeu.12395
       
  • Diversity and Evolution of Paramoeba spp. and their Kinetoplastid
           Endosymbionts

    • Authors: Shannon J. Sibbald; Ugo Cenci, Morgan Colp, Yana Eglit, Charles J. O'Kelly, John M. Archibald
      Abstract: Members of the genus Paramoeba (including Neoparamoeba) (Amoebozoa) are single-celled eukaryotes of economic and ecological importance because of their association with disease in a variety of marine animals including fish, sea urchins, and lobster. Interestingly, they harbor a eukaryotic endosymbiont of kinetoplastid ancestry, Perkinsela sp. To investigate the complex relationship between Paramoeba spp. and Perkinsela sp., as well as the relationships between different Paramoeba species, molecular data was obtained for four novel isolates. We also acquired new data from the urchin pathogen P. invadens. Comprehensive molecular phylogenetic analyses were carried out using 33 newly obtained 18S rDNA sequences from the host amoebae and 16 new 18S rDNA sequences from their corresponding Perkinsela sp., together with all publicly available 18S molecular data. Intra-isolate 18S rDNA nucleotide diversity was found to be surprisingly high within the various species of Paramoeba, but relatively low within their Perkinsela sp. endosymbionts. 18S rDNA phylogenies and ParaFit co-evolution analysis revealed a high degree of congruence between the Paramoeba and Perkinsela sp. tree topologies, strongly suggesting that a single endosymbiotic event occurred in the common ancestor of known Paramoeba species, and that the endosymbionts have been inherited vertically ever since.
      PubDate: 2017-02-27T03:15:47.457782-05:
      DOI: 10.1111/jeu.12394
       
  • Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through
           Protoplast Technology

    • Authors: Rachel A. Levin; David J. Suggett, Matthew R. Nitschke, Madeleine J.H. Oppen, Peter D. Steinberg
      Abstract: Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular, the generation of viable cells with their cell walls removed (termed protoplasts) has enabled a wide range of experimental techniques for bacteria, fungi, plants, and algae such as ultrastructure studies, virus infection studies, patch clamping, genetic transformation, and protoplast fusion. However, previous studies have struggled to remove the cell walls from armored dinoflagellates, potentially due to the internal placement of their cell walls. Here, we produce the first Symbiodinium protoplasts from three genetically and physiologically distinct strains via incubation with cellulase and osmotic agents. Digestion of the cell walls was verified by a lack of Calcofluor White fluorescence signal and by cell swelling in hypotonic culture medium. Fused protoplasts were also observed, motivating future investigation into intra- and inter-specific somatic hybridization of Symbiodinium. Following digestion and transfer to regeneration medium, protoplasts remained photosynthetically active, regrew cell walls, regained motility, and entered exponential growth. Generation of Symbiodinium protoplasts opens exciting, new avenues for researching these crucial symbiotic dinoflagellates, including genetic modification.
      PubDate: 2017-02-21T22:30:45.734813-05:
      DOI: 10.1111/jeu.12393
       
  • The First Isolation and Characterisation of the Protist Labyrinthula sp.
           in Southeastern Australia

    • Authors: Brooke K. Sullivan; Katie L. Robinson, Stacey M. Trevathan-Tackett, Erna S. Lilje, Frank H. Gleason, Osu Lilje
      Abstract: As a result of anthropogenic influences and global climate change, emerging infectious marine diseases are thought to be increasingly more common and more severe than in the past. The aim of our investigation was to confirm the presence of Labyrinthula, the aetiological agent of the seagrass wasting disease, in Southeastern Australia and provide the first isolation and characterisation of this protist, in Australia. Colonies and individual cells were positively identified as Labyrinthula using published descriptions, diagrams, and photographs. Their identity was then confirmed using DNA barcoding of a region of the 18S rRNA gene. Species level identification of isolates was not possible as the taxonomy of the Labyrinthula is still poorly resolved. Still, a diversity of Labyrinthula was isolated from small sections of the southeast coast of Australia. The isolates were grouped into three haplotypes that are biogeographically restricted. These haplotypes are closely related to previously identified saprotrophic clades. The study highlights the need for further investigation into the global distribution of Labyrinthula, including phylogenetic pathogenicity and analysis of host-parasite interactions in response to stressors. Given the results of our analyses, it is prudent to continue research into disease and epidemic agents to better prepare researchers for potential future outbreaks.
      PubDate: 2017-02-20T12:40:34.674614-05:
      DOI: 10.1111/jeu.12387
       
  • Beyond the “Code”: A Guide to the Description and Documentation of
           Biodiversity in Ciliated Protists (Alveolata, Ciliophora)

    • Authors: Alan Warren; David J. Patterson, Micah Dunthorn, John C. Clamp, Undine E.M. Achilles-Day, Erna Aescht, Saleh A. Al-Farraj, Saleh Al-Quraishy, Khaled Al-Rasheid, Martin Carr, John G. Day, Marc Dellinger, Hamed A. El-Serehy, Yangbo Fan, Feng Gao, Shan Gao, Jun Gong, Renu Gupta, Xiaozhong Hu, Komal Kamra, Gaytha Langlois, Xiaofeng Lin, Diana Lipscomb, Christopher S. Lobban, Pierangelo Luporini, Denis H. Lynn, Honggang Ma, Miroslav Macek, Jacqueline Mackenzie-Dodds, Seema Makhija, Robert I. Mansergh, Mercedes Martín-Cereceda, Nettie McMiller, David J.S. Montagnes, Svetlana Nikolaeva, Geoffrey Odhiambo Ong'ondo, Blanca Pérez-Uz, Jasmine Purushothaman, Pablo Quintela-Alonso, Johana Rotterová, Luciana Santoferrara, Chen Shao, Zhuo Shen, Xinlu Shi, Weibo Song, Thorsten Stoeck, Antonietta La Terza, Adriana Vallesi, Mei Wang, Thomas Weisse, Krzysztof Wiackowski, Lei Wu, Kuidong Xu, Zhenzhen Yi, Rebecca Zufall, Sabine Agatha
      Abstract: Recent advances in molecular technology have revolutionized research on all aspects of the biology of organisms, including ciliates, and created unprecedented opportunities for pursuing a more integrative approach to investigations of biodiversity. However, this goal is complicated by large gaps and inconsistencies that still exist in the foundation of basic information about biodiversity of ciliates. The present paper reviews issues relating to the taxonomy of ciliates and presents specific recommendations for best practice in the observation and documentation of their biodiversity. This effort stems from a workshop that explored ways to implement six Grand Challenges proposed by the International Research Coordination Network for Biodiversity of Ciliates (IRCN-BC). As part of its commitment to strengthening the knowledge base that supports research on biodiversity of ciliates, the IRCN-BC proposes to populate The Ciliate Guide, an online database, with biodiversity-related data and metadata to create a resource that will facilitate accurate taxonomic identifications and promote sharing of data.
      PubDate: 2017-02-15T23:25:35.740329-05:
      DOI: 10.1111/jeu.12391
       
  • Pelagostrobilidium liui n. sp. (Ciliophora, Choreotrichida) from the
           Coastal Waters of Northeastern Taiwan and an Improved Description of
           Pelagostrobilidium minutum Liu et al., 2012

    • Authors: Po-Cheng Chen; Kuo-Ping Chiang, Sheng-Fang Tsai
      Abstract: The number of somatic kineties in Pelagostrobilidium ranges from 4 to 6 according to the present state of knowledge. This study investigates Pelagostrobilidium liui n. sp. using live observation, protargol stain, and small subunit rDNA data sequencing. Pelagostrobilidium liui n. sp. is characterized by having a spherical-shaped body, four somatic kineties, with kinety 2 spiraled around the left side of body, about six elongated external membranelles, and invariably no buccal membranelle. It differs from its most similar congener, Pelagostrobilidium minutum Liu et al., , in (i) cell shape; (ii) macronucleus width; (iii) oral apparatus; (iv) anterior orientation of kinety 2; (v) location where kinety 2 commences; (vi) arrangement of kinety 1; (vii) distance between the anterior cell end and the locations where kineties commence; and (viii) the presence of 12 different bases (including two deletions) in the small subunit rDNA sequences. The diagnosis of P. minutum Liu et al., is also improved to include the following new characteristics: invariably four somatic kineties; kineties 2 and 4 alone commence at the same level; kinety 2 originates from right anterior cell half on ventral side, extends sinistrally posteriorly, over kinety 1, around left posterior region, terminates near posterior cell end on dorsal side; kinety 1 commences below anterior third of kinety 2.
      PubDate: 2017-02-10T02:32:25.05991-05:0
      DOI: 10.1111/jeu.12392
       
  • Biofilms for Monitoring Presence of Microsporidia in Environmental Water

    • Authors: Joseph A. Moss; Richard A. Snyder
      Abstract: The development of molecular methodologies for targeting pathogens such as the Microsporidia has greatly improved our monitoring capabilities and initiatives. This study analyzed samples collected from five locations in Pensacola, Florida, USA for the presence of Microsporidian pathogens. To circumvent various impediments associated with water collection and filtration, we utilized biofilms as sentinels for detection of Microsporidia. We implemented membrane-dissolution and sample purification in a single confined step followed by real-time PCR to confirm pathogen presence. The results of this study demonstrate that microsporidia are present in environmental water sites in the Florida panhandle and that biofilms may serve as another alternative mode to circumvent filtration methods for their detection.
      PubDate: 2017-01-29T23:51:45.647679-05:
      DOI: 10.1111/jeu.12390
       
  • Haptophyte Diversity and Vertical Distribution Explored by 18S and 28S
           Ribosomal RNA Gene Metabarcoding and Scanning Electron Microscopy

    • Authors: Sandra Gran-Stadniczeñko; Luka Šupraha, Elianne D. Egge, Bente Edvardsen
      Abstract: Haptophyta encompasses more than 300 species of mostly marine pico- and nanoplanktonic flagellates. Our aims were to investigate the Oslofjorden haptophyte diversity and vertical distribution by metabarcoding, and to improve the approach to study haptophyte community composition, richness and proportional abundance by comparing two rRNA markers and scanning electron microscopy (SEM). Samples were collected in August 2013 at the Outer Oslofjorden, Norway. Total RNA/cDNA was amplified by haptophyte-specific primers targeting the V4 region of the 18S, and the D1-D2 region of the 28S rRNA. Taxonomy was assigned using curated haptophyte reference databases and phylogenetic analyses. Both marker genes showed Chrysochromulinaceae and Prymnesiaceae to be the families with highest number of Operational Taxonomic Units (OTUs), as well as proportional abundance. The 18S rRNA data set also contained OTUs assigned to eight supported and defined clades consisting of environmental sequences only, possibly representing novel lineages from family to class. We also recorded new species for the area. Comparing coccolithophores by SEM with metabarcoding shows a good correspondence with the 18S rRNA gene proportional abundances. Our results contribute to link morphological and molecular data and 28S to 18S rRNA gene sequences of haptophytes without cultured representatives, and to improve metabarcoding methodology.
      PubDate: 2017-01-29T23:47:03.038212-05:
      DOI: 10.1111/jeu.12388
       
  • Molecular Phylogeny of Paraphelidium letcheri sp. nov. (Aphelida,
           Opisthosporidia)

    • Authors: Sergey A. Karpov; Guifré Torruella, David Moreira, Maria A. Mamkaeva, Purificación López-García
      PubDate: 2017-01-22T07:25:35.739847-05:
      DOI: 10.1111/jeu.12389
       
  • Issue Information

    • First page: 73
      PubDate: 2017-03-31T14:22:34.7424-05:00
      DOI: 10.1002/bes2.1270
       
  • Ecological Society of America Announces 2017 Fellows

    • First page: 77
      PubDate: 2017-03-31T14:22:35.469739-05:
      DOI: 10.1002/bes2.1309
       
  • 2016 ESA Election Results

    • First page: 82
      PubDate: 2017-03-31T14:22:34.609653-05:
      DOI: 10.1002/bes2.1305
       
  • Request for Student Award Judges

    • First page: 83
      PubDate: 2017-03-31T14:22:34.299936-05:
      DOI: 10.1002/bes2.1318
       
  • ESA Historical Records Committee (Established 1944) Newsletter

    • First page: 84
      PubDate: 2017-03-31T14:22:35.296115-05:
      DOI: 10.1002/bes2.1311
       
  • Minutes of the ESA Governing Board 8–9 November 2016 Washington,
           D.C.

    • First page: 87
      PubDate: 2017-03-31T14:22:34.364671-05:
      DOI: 10.1002/bes2.1306
       
  • Breaking Through Ecosystem Boundaries

    • Authors: Amanda Klemmer; Michel Loreau
      First page: 95
      PubDate: 2017-03-31T14:22:36.376256-05:
      DOI: 10.1002/bes2.1313
       
  • Breaking Through Ecosystem Boundaries: Editor's Note

    • Authors: Stephen L. Young
      First page: 95
      PubDate: 2017-03-31T14:22:35.687288-05:
      DOI: 10.1002/bes2.1310
       
  • How Many Limnologists Does it Take to Fix the Plumbing' Editor's Note

    • Authors: Stephen L. Young
      First page: 99
      PubDate: 2017-03-31T14:22:36.461095-05:
      DOI: 10.1002/bes2.1312
       
  • How Many Limnologists Does It Take to Fix the Plumbing' The Arising
           Researcher

    • Authors: Grace Wilkinson
      First page: 99
      PubDate: 2017-03-31T14:22:34.523423-05:
      DOI: 10.1002/bes2.1317
       
  • How Many Limnologists Does It Take to Fix the Plumbing' The
           Established Researcher

    • Authors: Jonathan J. Cole
      First page: 100
      PubDate: 2017-03-31T14:22:35.600276-05:
      DOI: 10.1002/bes2.1316
       
  • Ecology on the Runway: Engaging the Public in Unexpected Places

    • Authors: Nalini Nadkarni; Douglas Levey
      First page: 103
      PubDate: 2017-03-31T14:22:34.819909-05:
      DOI: 10.1002/bes2.1302
       
  • Picture the Past, Looking to the Future: The Forest History Society's
           Repeat Photography Portal

    • Authors: Sara Pezzoni
      First page: 110
      PubDate: 2017-03-31T14:22:34.989786-05:
      DOI: 10.1002/bes2.1307
       
  • History of Ecological Sciences, Part 58B: Marine Ecology, mid-1920s to
           about 1990: Carson, Riley, Cousteau, and Clark

    • Authors: Frank N. Egerton
      First page: 113
      PubDate: 2017-03-31T14:22:35.724275-05:
      DOI: 10.1002/bes2.1314
       
  • The End of the Mythical Giant Catfish

    • Authors: Stéphanie Boulêtreau; Frédéric Santoul
      First page: 150
      PubDate: 2017-03-31T14:22:35.413301-05:
      DOI: 10.1002/bes2.1304
       
  • Caterpillar Seed Predators Mediate Shifts in Selection on Flowering
           Phenology in Their Host Plant

    • Authors: Alicia Valdés; Johan Ehrlén
      First page: 153
      PubDate: 2017-03-31T14:22:34.658928-05:
      DOI: 10.1002/bes2.1303
       
  • Global Change Impact in the Sierra Nevada Long-Term Ecological Research
           Site (Southern Spain)

    • Authors: Regino Zamora; Antonio J. Pérez-Luque, Francisco J. Bonet, José Miguel Barea-Azcón, Rut Aspizua, Francisco J. Sánchez-Gutiérrez, Francisco J. Cano-Manuel, Blanca Ramos-Losada, Ignacio Henares-Civantos
      First page: 157
      PubDate: 2017-03-31T14:22:35.096278-05:
      DOI: 10.1002/bes2.1308
       
  • As Climate Shifts, So Do Pests: a National Forum and Assessment

    • Authors: Stephen L. Young
      First page: 165
      PubDate: 2017-03-31T14:22:34.084182-05:
      DOI: 10.1002/bes2.1315
       
 
 
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