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- Histochemical and Phytochemical Analysis of Medicinally Important Plants
Authors: B. K. Sushma, K. S. Ashalatha, Preetam Ray, H. R. Raveesha
Pages: 1 - 13
Abstract: The present study was aimed to investigate the histochemical and phytochemical studies of four important medicinal plants. Leaf, stem and root sections were examined for alkaloid, protein and phenolic tests. Methanolic extracts of leaf, stem and root were analysed for the phytochemical screening of major bioactive constituents. Histochemical analysis reveals the presence of alkaloids, phenols and proteins in epidermis, cortex and vascular bundles of root, stem and leaf. Preliminary phytochemical analysis showed the presence of alkaloids, tannins, phenols, flavonoids, steroids, saponins, terpenoids, coumarins, quinone and proteins. The root extract of Catharanthus roseus recorded maximum content of phenols compared to other extracts. Costus pictus root and leaf extracts exhibited a significant amount of flavonoids and tannins respectively. Further studies were focused on isolation and characterization of each plant compounds.
PubDate: 2020-01-04
DOI: 10.9734/ejmp/2019/v30i430185
- Assessment of Growth Characteristics of Medicinal Plants Ocimum
tenuiflorum L. and Plectranthus amboinicus (Lour.) Spreng. Under Shade Net
and Open Conditions
Authors: J. Ambujavalli, K. Santhiya, K. Rohini, V. Kumaresan
Pages: 1 - 8
Abstract: Aim: To study the effect of shade net on the growth characteristics of two medicinal plants viz., Tulsi and Indian Borage in terms of plant height, number of leaves, leaf area and flowering in relation to open condition. Place and Duration of Study: Lawspet, Puducherry, India between June 2018 and October 2018. Methodology: Tulsi and Indian Borage plants were grown in similar type of soil. The potted plants were kept in open, 35% and 75% shade net conditions and studied for 3 months. The plant height, number of leaves, and leaf area were measured including number of inflorescence branches for Tulsi. Results: The average air temperature was lower under shade net. Relative humidity was more in shade net conditions with maximum in 75% shade and least in open conditions. The number of leaves of Tulsi and Indian Borage recorded was maximum when grown under 35% shade and least in open conditions. Plant height was maximum in 75% shade for Tulsi and 35% shade in Indian Borage and least in open conditions on both the cases. Leaf area was maximum for both Tulsi and Indian Borage in 75% shade. Conclusion: Growth of the two medicinal plants was influenced by the microclimate conditions. The present study shows that the medicinal plants can be grown in shade net conditions to increase the yield of medicinally useful parts.
PubDate: 2020-01-04
DOI: 10.9734/ejmp/2019/v30i430186
- Anti-oxidant and Anti-inflammatory Activity of Ethyl Acetate Fraction of
Moringa oleifera Flowers
Authors: A. Rajeshkanna, M. M. Senthamilselvi, D. Prabhakaran
Pages: 1 - 8
Abstract: Aims: To investigate the antioxidant and anti-inflammatory activities of ethyl acetate extract of Moringa oleifera flowers. Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between April 2017 and January 2018. Methodology: Extraction and fractionation were carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The anti-inflammatory effect of the extract was investigated by HRBC membrane stabilization and Albumin denaturation methods. Anti-oxidant effect of the extract was determined by DPPH assay and ABTS method. Results: The dry sample extracted from the ethyl acetate fraction of Moringa oleifera flowers possess highly anti-oxidant activity showed by the DPPH assay and ABTS method and also having anti-inflammatory activity is determined by human red blood cell (HRBC) membrane stabilization and Albumin denaturation methods. However, these effects need to be confirmed using in vivo models and clinical trials before its utilization as a therapeutic agent. Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Moringa oleifera flowers possesses effective anti-oxidant and anti-inflammatory activities.
PubDate: 2020-01-03
DOI: 10.9734/ejmp/2019/v30i430184
- Quality of Guava Seed (Psidium guajava L.) White and Red Varieties on
Different Substrates
Authors: Calebe Oliveira Crispim da Silva, Daniella Inácio Barros, Helber Véras Nunes, Bruno Henrique Di Napoli Nunes, João Lucas Aires Dias, Vitória Alves Cavalcante, Higor Silva Rezende, Igor Fayder de Souza e Silva, Dhenys Miller Jorge de Souza, Victor Hugo Silva Rezende
Pages: 1 - 5
Abstract: The guava tree is the fruit of the guava tree of the species Psidium guajava L. belonging to the family of Myrtaceae a rustic fruit and adapts to the most varied types of soils. It is a fleshy fruit, thin-skinned, green, or when mature yellowish and very rich in soluble fibers. Seed germination is influenced by factors such as substrate, which can improve germination, resulting in the acquisition of more vigorous seedlings. The experiment was conducted in a greenhouse at the Federal Institute of Education, Science and Technology of Tocantins, Gurupi/TO, in 2017. The seeds of guava Kumagai and Paluma (Psidium guajava L.) used in the experiment were taken directly from the fruit, which was collected in the urban region of Gurupi in March 2017. For the two varieties evaluated, both for the first emergence count and seedling emergence, the substrates stood out: organic compound + black earth + commercial substrate (55% and 37%) (67% and 49%), washed sand + rice straw + black earth (52% and 36%) (52% and 36 %) lower substrates: washed sand (46% and 10%) (66% and 35%) and rice straw + sawdust + black ground (47% and 7%) (59% and 31%), respectively. The substrates: organic compound + black earth + commercial substrate and washed sand + rice straw + black earth provided the highest values of viability and vigour in white and red pulp guava seeds.
PubDate: 2020-01-01
DOI: 10.9734/ejmp/2019/v30i430183
- Synthesis and Characterization of Indium Tin Oxide with Neem Extract for
Antioxidant Applications
Authors: R. Perumalsamy, G. Prabhavathi, D. Saravanakkumar, N. N. Shafeera, A. Ayeshamariam, M. Sivabharathy, M. Jayachandran
Pages: 1 - 9
Abstract: Indium tin oxide was prepared by using simple co precipitation method with Neem extract as reducing agent and it was characterized by using X-ray diffractometer, with the applications of antioxidant effect, the prepared sample was directly calcined at 400ºC and then characterized. Morphological studies were analyzed by using Transmission Electron Microscopy and Selective area diffraction pattern. The crystal sizes were calculated and it value is nearly 12 nm. Here, Williamson-Hall (W–H) have been used to investigate the particle size and the intrinsic strain from the XRD peak broadening analysis.
PubDate: 2019-12-31
DOI: 10.9734/ejmp/2019/v30i430182
- Bio-guided Fractionation of the Ethanolic Extract from Leaves of Trema
orientalis Blume (Cannabaceae), a Presumed Antihypertensive Plant from
Congo-Brazzaville
Authors: Bonazaba Milandou Longin Justin Clair, Nkounkou Loumpangou Célestine, Carreyre Hélène, Bescond Jocelyn, Vandebrouck Clarisse, Etou Ossibi Arnaud Wilfrid, Moutsambote Jean-Marie, Ouamba Jean-Maurille, Thibaudeau Sébastien, Abena Ange Antoine
Pages: 1 - 14
Abstract: Hypertension is a risk factor for cardiovascular disease, which is currently a real public health problem. This disease affects about one billion people worldwide and is responsible for more than 70% of cardiovascular related deaths. Recently, the World Health Organization reported that of the hypertensive cases detected in Congo, only 7% were controlled. Today, there is no lifetime treatment and existing drugs are less accessible by the African population. To treat the disease, the Congolese population uses more the medicinal plants. However, the majority of compounds responsible for the biological activity of these plants are not known. In order to bring out Congolese plants with antihypertensive properties, we focus our interest on Trema orientalis Blume (Canabaceae). An ethanolic extract of the leaves of Trema orientalis was prepared after successive depletion of the organic solvents. Thereafter, a bio-guided fractionation on silica gel of the ethanol extract was carried out. Fractionation monitoring was done by TLC and the results of vasodilating activity measured. The fractions exhibiting the best biological activity allowed a second fractionation process to obtain five fractions which are characteristic of polyphenols, in particular flavonoids, and which exhibited good vasodilating activity on the isolated aorta of rats. Our future work will focus on the identification of these biologically active compounds.
PubDate: 2019-12-30
DOI: 10.9734/ejmp/2019/v30i430181
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