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  Subjects -> VETERINARY SCIENCE (Total: 176 journals)
Acta Scientiae Veterinariae     Open Access  
Acta Veterinaria     Open Access  
Acta Veterinaria Brno     Open Access   (Followers: 1)
Acta Veterinaria Hungarica     Full-text available via subscription   (Followers: 1)
Acta Veterinaria Scandinavica     Open Access   (Followers: 1)
Advances in Animal Biosciences     Full-text available via subscription   (Followers: 5)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 5)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 10)
American Journal of Animal and Veterinary Sciences     Open Access   (Followers: 7)
American Journal of Primatology     Hybrid Journal   (Followers: 5)
American Journal of Veterinary Research     Full-text available via subscription   (Followers: 14)
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C     Hybrid Journal   (Followers: 2)
Animal Behaviour     Hybrid Journal   (Followers: 201)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 6)
Animal Health Research Reviews     Hybrid Journal   (Followers: 4)
Animal Reproduction Science     Hybrid Journal   (Followers: 4)
Animals     Open Access   (Followers: 5)
Annales UMCS, Medicina Veterinaria     Open Access  
Annals of Agricultural and Environmental Medicine     Open Access   (Followers: 1)
Annual Review of Animal Biosciences     Full-text available via subscription   (Followers: 4)
Anthrozoos : A Multidisciplinary Journal of The Interactions of People & Animals     Full-text available via subscription   (Followers: 7)
Archives of Animal Nutrition     Hybrid Journal   (Followers: 4)
Archivos de Medicina Veterinaria     Open Access   (Followers: 1)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access   (Followers: 1)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atatürk Üniversitesi Veteriner Bilimleri Dergisi     Open Access  
Australian Equine Veterinarian     Full-text available via subscription   (Followers: 1)
Australian Veterinary Journal     Hybrid Journal   (Followers: 10)
Avances en Ciencias Veterinarias     Open Access  
Avian Diseases     Full-text available via subscription   (Followers: 4)
Avian Diseases Digest     Full-text available via subscription   (Followers: 3)
Avian Pathology     Hybrid Journal   (Followers: 2)
Bangladesh Journal of Animal Science     Open Access  
Bangladesh Journal of Veterinary Medicine     Open Access  
BMC Veterinary Research     Open Access   (Followers: 5)
Brazilian Journal of Veterinary Research and Animal Science     Open Access   (Followers: 6)
Bulletin of Animal Health and Production in Africa     Full-text available via subscription  
Bulletin of the Veterinary Institute in Pulawy     Open Access  
Canadian Journal of Veterinary Research     Full-text available via subscription   (Followers: 4)
Case Reports in Veterinary Medicine     Open Access   (Followers: 4)
Ciência Rural     Open Access   (Followers: 2)
Companion Animal     Full-text available via subscription   (Followers: 4)
Domestic Animal Endocrinology     Hybrid Journal   (Followers: 3)
Equine Health     Full-text available via subscription  
Equine Veterinary Education     Hybrid Journal   (Followers: 7)
Equine Veterinary Journal     Hybrid Journal   (Followers: 9)
Ethiopian Veterinary Journal     Open Access   (Followers: 2)
Eurasian Journal of Veterinary Sciences     Open Access   (Followers: 1)
Human & Veterinary Medicine - International Journal of the Bioflux Society     Open Access   (Followers: 5)
ILAR Journal     Hybrid Journal  
In Practice     Full-text available via subscription   (Followers: 3)
Indian Journal of Animal Sciences     Open Access   (Followers: 5)
Indian Journal of Veterinary Anatomy     Full-text available via subscription   (Followers: 3)
International Journal for Agro Veterinary and Medical Sciences     Open Access   (Followers: 3)
International Journal of Livestock Research     Open Access  
International Journal of Veterinary Science and Medicine     Open Access   (Followers: 2)
InVet     Open Access  
Irish Veterinary Journal     Open Access   (Followers: 2)
ISRN Veterinary Science     Open Access  
Journal of Veterinary Science & Technology     Open Access   (Followers: 3)
Journal of Advanced Veterinary and Animal Research     Open Access   (Followers: 4)
Journal of Animal Behaviour and Biometeorology     Open Access   (Followers: 1)
Journal of Animal Physiology and Animal Nutrition     Hybrid Journal   (Followers: 5)
Journal of Applied Animal Nutrition     Hybrid Journal   (Followers: 3)
Journal of Avian Medicine and Surgery     Full-text available via subscription   (Followers: 4)
Journal of Equine Veterinary Science     Hybrid Journal   (Followers: 8)
Journal of Exotic Pet Medicine     Full-text available via subscription   (Followers: 2)
Journal of Experimental and Applied Animal Sciences     Open Access   (Followers: 1)
Journal of Feline Medicine & Surgery     Hybrid Journal   (Followers: 4)
Journal of Research in Forestry, Wildlife and Environment     Open Access  
Journal of Small Animal Practice     Hybrid Journal   (Followers: 8)
Journal of the American Veterinary Medical Association     Full-text available via subscription   (Followers: 21)
Journal of the Hellenic Veterinary Medical Society     Full-text available via subscription   (Followers: 2)
Journal of the South African Veterinary Association     Open Access   (Followers: 1)
Journal of Venomous Animals and Toxins     Open Access   (Followers: 3)
Journal of Veterinary Advances     Open Access   (Followers: 4)
Journal of Veterinary Behavior: Clinical Applications and Research     Hybrid Journal   (Followers: 3)
Journal of Veterinary Cardiology     Full-text available via subscription   (Followers: 4)
Journal of Veterinary Diagnostic Investigation     Hybrid Journal   (Followers: 4)
Journal of Veterinary Emergency and Critical Care     Hybrid Journal   (Followers: 10)
Journal of Veterinary Internal Medicine     Hybrid Journal   (Followers: 12)
Journal of Veterinary Medical Education     Partially Free   (Followers: 8)
Journal of Veterinary Medicine     Open Access   (Followers: 4)
Journal of Veterinary Medicine and Animal Health     Open Access   (Followers: 2)
Journal of Veterinary Pharmacology and Therapeutics     Hybrid Journal   (Followers: 4)
Journal of Veterinary Science & Medical Diagnosis     Full-text available via subscription   (Followers: 1)
Journal of Zoo and Aquarium Research     Open Access  
Journal of Zoo and Wildlife Medicine     Full-text available via subscription   (Followers: 2)
Kenya Veterinarian     Full-text available via subscription   (Followers: 1)
kleintier konkret     Hybrid Journal  
Livestock     Full-text available via subscription   (Followers: 1)
Macedonian Veterinary Review     Open Access   (Followers: 3)
MEDIA PETERNAKAN - Journal of Animal Science and Technology     Open Access   (Followers: 1)
Medical Mycology     Open Access   (Followers: 3)
Medical Mycology Case Reports     Open Access  
Microbes and Health     Open Access   (Followers: 2)
New Zealand Veterinary Journal     Full-text available via subscription   (Followers: 7)
New Zealand Veterinary Nurse     Full-text available via subscription   (Followers: 2)
Nigerian Veterinary Journal     Open Access  
Onderstepoort Journal of Veterinary Research     Open Access   (Followers: 2)

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Journal Cover Journal of Veterinary Diagnostic Investigation
   Journal TOC RSS feeds Export to Zotero [6 followers]  Follow    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
     ISSN (Print) 1040-6387 - ISSN (Online) 1943-4936
     Published by Sage Publications Homepage  [739 journals]   [SJR: 0.627]   [H-I: 51]
  • Methods comparison: An alternative approach for evaluating the impact of a
           modification to a validated assay
    • Authors: Reising, M. M; Schumann, K. R, Crossley, B. M, Glas, P. S, Jacobson, R. H, Toohey-Kurth, K. L, Pedersen, J. C, Siev, D, Martin, B. M.
      Pages: 480 - 487
      Abstract: The current report discusses the process in which a methods comparison study in the National Animal Health Laboratory Network is performed. Specific details are provided for designing and analyzing studies intended to evaluate analytical sensitivity, efficiency, analytical specificity, cross-contamination, repeatability, operator variability, and to compare the performance of methods using diagnostic samples. As an example, a case study is presented comparing the performance of a candidate reverse transcription polymerase chain reaction (RT-PCR) chemistry to the current RT-PCR chemistry in use when the assay was originally validated. The present study revealed that, for all of the validation factors evaluated, the candidate method performed at least as well and generally better than the current method. The candidate method was, therefore, deemed fit for the original intended purpose of the current method and rendered acceptable for use. A discussion of the case study is intended to further motivate consideration of the study designs chosen.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714535402|hwp:master-id:spvdi;1040638714535402
      Issue No: Vol. 26, No. 4 (2014)
  • Molecular characterization and virulence gene profiling of pathogenic
           Streptococcus agalactiae populations from tilapia (Oreochromis sp.) farms
           in Thailand
    • Authors: Kayansamruaj, P; Pirarat, N, Katagiri, T, Hirono, I, Rodkhum, C.
      Pages: 488 - 495
      Abstract: Streptococcus spp. were recovered from diseased tilapia in Thailand during 2009–2010 (n = 33), and were also continually collected from environmental samples (sediment and water) from tilapia farms for 9 months in 2011 (n = 25). The relative percent recovery of streptococci from environmental samples was 13–67%. All streptococcal isolates were identified as S. agalactiae (group B streptococci [GBS]) by a species-specific polymerase chain reaction. In molecular characterization assays, 4 genotypic categories comprised of 1) molecular serotypes, 2) the infB allele, 3) virulence gene profiling patterns (cylE, hylB, scpB, lmb, cspA, dltA, fbsA, fbsB, bibA, gap, and pili backbone–encoded genes), and 4) randomly amplified polymorphic DNA (RAPD) fingerprinting patterns, were used to describe the genotypic diversity of the GBS isolates. There was only 1 isolate identified as molecular serotype III, while the others were serotype Ia. Most GBS serotype Ia isolates had an identical infB allele and virulence gene profiling patterns, but a large diversity was established by RAPD analysis with diversity tending to be geographically dependent. Experimental infection of Nile tilapia (Oreochromis niloticus) revealed that the GBS serotype III isolate was nonpathogenic in the fish, while all 5 serotype Ia isolates (3 fish and 2 environmental isolates) were pathogenic, with a median lethal dose of 6.25–7.56 log10 colony-forming units. In conclusion, GBS isolates from tilapia farms in Thailand showed a large genetic diversity, which was associated with the geographical origins of the bacteria.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714534237|hwp:master-id:spvdi;1040638714534237
      Issue No: Vol. 26, No. 4 (2014)
  • Development of a multiplex amplification refractory mutation system
           reverse transcription polymerase chain reaction assay for the differential
           diagnosis of Feline leukemia virus vaccine and wild strains
    • Authors: Ho, C.-F; Chan, K.-W, Yang, W.-C, Chiang, Y.-C, Chung, Y.-T, Kuo, J, Wang, C.-Y.
      Pages: 496 - 506
      Abstract: A multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (ARMS RT-PCR) was developed for the differential diagnosis of Feline leukemia virus (FeLV) vaccine and wild-type strains based on a point mutation between the vaccine strain (S) and the wild-type strain (T) located in the p27 gene. This system was further upgraded to obtain a real-time ARMS RT-PCR (ARMS qRT-PCR) with a high-resolution melt analysis (HRMA) platform. The genotyping of various strains of FeLV was determined by comparing the HRMA curves with the defined wild-type FeLV (strain TW1), and the results were expressed as a percentage confidence. The detection limits of ARMS RT-PCR and ARMS qRT-PCR combined with HRMA were 100 and 1 copies of transcribed FeLV RNA per 0.5 ml of sample, respectively. No false-positive results were obtained with 6 unrelated pathogens and 1 feline cell line. Twelve FeLV Taiwan strains were correctly identified using ARMS qRT-PCR combined with HRMA. The genotypes of the strains matched the defined FeLV wild-type strain genotype with at least 91.17% confidence. A higher degree of sequence polymorphism was found throughout the p27 gene compared with the long terminal repeat region. In conclusion, the current study describes the phylogenetic relationship of the FeLV Taiwan strains and demonstrates that the developed ARMS RT-PCR assay is able to be used to detect the replication of a vaccine strain that has not been properly inactivated, thus acting as a safety check for the quality of FeLV vaccines.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714534850|hwp:master-id:spvdi;1040638714534850
      Issue No: Vol. 26, No. 4 (2014)
  • Brucella placentitis and seroprevalence in northern fur seals (Callorhinus
           ursinus) of the Pribilof Islands, Alaska
    • Authors: Duncan, C. G; Tiller, R, Mathis, D, Stoddard, R, Kersh, G. J, Dickerson, B, Gelatt, T.
      Pages: 507 - 512
      Abstract: Brucella species infect a wide range of hosts with a broad spectrum of clinical manifestations. In mammals, one of the most significant consequences of Brucella infection is reproductive failure. There is evidence of Brucella exposure in many species of marine mammals, but the outcome of infection is often challenging to determine. The eastern Pacific stock of northern fur seals (NFSs, Callorhinus ursinus) has declined significantly, spawning research into potential causes for this trend, including investigation into reproductive health. The objective of the current study was to determine if NFSs on St. Paul Island, Alaska have evidence of Brucella exposure or infection. Archived DNA extracted from placentas (n = 119) and serum (n = 40) samples were available for testing by insertion sequence (IS) 711 polymerase chain reaction (PCR) and the Brucella microagglutination test (BMAT), respectively. As well, placental tissue was available for histologic examination. Six (5%) placentas were positive by PCR, and a single animal had severe placentitis. Multilocus variable number tandem repeat analysis profiles were highly clustered and closely related to other Brucella pinnipedialis isolates. A single animal was positive on BMAT, and 12 animals had titers within the borderline range; 1 borderline animal was positive by PCR on serum. The findings suggest that NFSs on the Pribilof Islands are exposed to Brucella and that the organism has the ability to cause severe placental disease. Given the population trend of the NFS, and the zoonotic nature of this pathogen, further investigation into the epidemiology of this disease is recommended.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714532647|hwp:master-id:spvdi;1040638714532647
      Issue No: Vol. 26, No. 4 (2014)
  • Feline spinal cord gliomas: Clinicopathologic and diagnostic features of
           seven cases
    • Authors: Hammond, J. J; deLahunta, A, Glass, E. N, Kent, M, Summers, B. A, Miller, A. D.
      Pages: 513 - 520
      Abstract: Intraparenchymal spinal cord tumors in the cat are rarely reported and often as single case reports. In the current study, the clinical, magnetic resonance imaging (MRI), histologic, and immunohistochemical features of 7 cases of intraparenchymal spinal cord tumors in the cat are described. All cats were domestic breed, ranged from 4 to 12 years of age (median 8 years), and included spayed females (5/7) and neutered males (2/7). The duration of clinical signs ranged from 2 weeks to 3 months. MRI revealed lesions that were hyperintense on T2-weighted images with variable contrast enhancement. All 7 tumors had histologic features consistent with glial origin: 3 were astrocytic (gemistocytic or fibrous), and 2 were oligoastrocytic. Single cases of oligodendroglioma and gliomatosis cerebri were also present in the study. Glial fibrillary acidic protein immunoreactivity was robust in the tumors that were predominately astrocytic, and the gliomatosis cerebri case had extensive BLA.36 and Iba1 immunoreactivity. Ki-67 immunoreactivity was variable and most abundant in the case of malignant oligoastrocytoma. The majority of peritumoral lymphocytes were CD3 positive. The current study expands upon the known reports of spinal cord neoplasia in the cat, confirms a caudal cervical segment predilection, and includes a report of gliomatosis cerebri in the spinal cord of a cat.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714533118|hwp:master-id:spvdi;1040638714533118
      Issue No: Vol. 26, No. 4 (2014)
  • A high-throughput biliverdin assay using infrared fluorescence
    • Authors: Berlec, A; Štrukelj, B.
      Pages: 521 - 526
      Abstract: Biliverdin is an intermediate of heme degradation with an established role in veterinary clinical diagnostics of liver-related diseases. The need for chromatographic assays has so far prevented its wider use in diagnostic laboratories. The current report describes a simple, fast, high-throughput, and inexpensive assay, based on the interaction of biliverdin with infrared fluorescent protein (iRFP) that yields functional protein exhibiting infrared fluorescence. The assay is linear in the range of 0–10 µmol/l of biliverdin, has a limit of detection of 0.02 μmol/l, and has a limit of quantification of 0.03 µmol/l. The assay is accurate with relative error less than 0.15, and precise, with coefficient of variation less than 5% in the concentration range of 2–9 µmol/l of biliverdin. More than 95% of biliverdin was recovered from biological samples by simple dimethyl sulfoxide extraction. There was almost no interference by hemin, although bilirubin caused an increase in the biliverdin concentration, probably due to spontaneous oxidation of bilirubin to biliverdin. The newly developed biliverdin assay is appropriate for reliable quantification of large numbers of samples in veterinary medicine.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714535403|hwp:master-id:spvdi;1040638714535403
      Issue No: Vol. 26, No. 4 (2014)
  • Comparison of two methods for measurement of equine insulin
    • Authors: Banse, H. E; McCann, J, Yang, F, Wagg, C, McFarlane, D.
      Pages: 527 - 530
      Abstract: Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r 2 = 0.99, P < 0.001) than the CIA (r 2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714536560|hwp:master-id:spvdi;1040638714536560
      Issue No: Vol. 26, No. 4 (2014)
  • A multiresidue screen for the analysis of toxicants in bovine rumen
    • Authors: Vudathala, D. K; Cummings, M. R, Murphy, L. A.
      Pages: 531 - 537
      Abstract: Analysis of rumen contents is helpful in solving poisoning cases when ingestion of a toxic substance by cattle or other ruminant animals is suspected. The most common technique employs extraction of the sample with organic solvent followed by clean-up method(s) before analysis with gas chromatography–mass spectrometry equipped with a library of mass spectra to help identify unknowns. A rapid method using magnesium sulfate, primary secondary amine, and C18 sorbents following principles of QuEChERS to clean up rumen contents samples is reported herein. The method was validated to analyze fortified bovine rumen contents to detect commonly found organophosphorus pesticides, carbamates, and several other compounds such as atropine, 4-aminopyridine, caffeine, scopolamine, 3-chloro-4-methylaniline, strychnine, metaldehyde, and metronidazole. For each compound, the ratio of 2 ions from the mass spectrum was monitored in fortified rumen contents. The ion ratio of fortified sample was compared with the ion ratio of standard sample spectrum and was found to be within 20%, with the exception of aldicarb and 4-aminopyridine with ion ratio of 26% and 29%, respectively. Usefulness of the method was demonstrated by not only analyzing bovine rumen contents but also canine and avian gastrointestinal contents submitted for organic chemical screening.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714541836|hwp:resource-id:spvdi;26/4/531
      Issue No: Vol. 26, No. 4 (2014)
  • Use of the polymerase chain reaction assay for the detection of Babesia
           odocoilei 18S ribosomal RNA in formalin-fixed tissues
    • Authors: Lockerbie, B. P; Bollinger, T. K, Burgess, H. J.
      Pages: 538 - 541
      Abstract: The effect of fixation and storage conditions on the performance of polymerase chain reaction (PCR) assays for Babesia odocoilei were examined using 3 different primer sets targeting the eukaryotic 18S ribosomal RNA gene, with variably sized products of 1,723 base pairs (bp), 483 bp, and 306 bp. All primer sets performed well on fresh-frozen tissue, and storage for 1 year at –20°C did not affect PCR performance. Formalin fixation markedly affected the amplicon length that could be amplified. However, DNA was successfully amplified after storage in formalin for 2 months using the primer set with a 483-bp product, and up to 6 months using the primer set with a 306-bp product. The latter primer set successfully differentiated B. odocoilei and Babesia microti DNA; however, further evaluation is required to confirm its specificity. Treatment of tissues with formic acid, at concentrations typically used to denature prions, degraded the DNA and made it unsuitable for PCR testing.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714535600|hwp:master-id:spvdi;1040638714535600
      Issue No: Vol. 26, No. 4 (2014)
  • Touchdown polymerase chain reaction detection of polycystic kidney disease
           and laboratory findings in different cat populations
    • Authors: Scalon, M. C; da Silva, T. F, Aquino, L. C, Carneiro, F. T, Lima, M. G. d. M, Lemos, M. d. S, Paludo, G. R.
      Pages: 542 - 546
      Abstract: Autosomal-dominant polycystic kidney disease (ADPKD) is the most prevalent inherited genetic disease of cats, predominantly affecting Persian and Persian-related cats. A point mutation (C->A transversion) in exon 29 of the PKD1 gene causes ADPKD, and is the specific molecular target for genetic diagnosis in cats. The current study describes a newly developed touchdown polymerase chain reaction (PCR) to detect this single point mutation, using 2 primers specific for the mutant allele, adapted from an existing multiplex amplification refractory mutation system (ARMS PCR). Furthermore, correlations between the clinical outcomes of tested animals and the results of the genetic test were investigated. A total of 334 cats were tested, 188 from the Veterinary Hospital of Small Animals at the University of Brasilia, and 146 from an anti-rabies vaccine campaign of the Federal District. A total prevalence of 9% was evident among the samples, with 33% of the Persian cats testing positive, and 7% of the Brazilian long- and shorthaired cats testing positive. Prevalence was not correlated with gender or hemogram. Positive animals exhibited hyperglobulinemia (P = 0.02). This research demonstrated that the mutation does not only occur in Persian and Persian-related cats, and that a touchdown PCR can be used to diagnose ADPKD.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714536561|hwp:master-id:spvdi;1040638714536561
      Issue No: Vol. 26, No. 4 (2014)
  • Molecular, biological, and antigenic characterization of a Border disease
           virus isolated from a pig during classical swine fever surveillance in
    • Authors: Nagai, M; Aoki, H, Sakoda, Y, Kozasa, T, Tominaga-Teshima, K, Mine, J, Abe, Y, Tamura, T, Kobayashi, T, Nishine, K, Tateishi, K, Suzuki, Y, Fukuhara, M, Ohmori, K, Todaka, R, Katayama, K, Mizutani, T, Nakamura, S, Kida, H, Shirai, J.
      Pages: 547 - 552
      Abstract: In the current study, molecular, biological, and antigenic analyses were performed to characterize Border disease virus (BDV) strain FNK2012-1 isolated from a pig in 2012 in Japan. The complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. Phylogenetic analysis revealed that FNK2012-1 was clustered into BDV genotype 1 with ovine strains. FNK2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine cells than in porcine cells. Specific pathogen–free pigs inoculated with FNK2012-1 did not show any clinical signs. Noninoculated contact control pigs also did not show clinical signs and did not seroconvert. The results suggest that FNK2012-1 may be of ruminant origin and is poorly adapted to pigs. Such observations can provide important insights into evidence for infection and transmission of BDV, which may be of ruminant origin, among pigs.
      PubDate: 2014-07-15T09:41:01-07:00
      DOI: 10.1177/1040638714541837|hwp:resource-id:spvdi;26/4/547
      Issue No: Vol. 26, No. 4 (2014)
  • Whole genome sequencing and phylogenetic analysis of Bluetongue virus
           serotype 2 strains isolated in the Americas including a novel strain from
           the western United States
    • Authors: Gaudreault, N. N; Mayo, C. E, Jasperson, D. C, Crossley, B. M, Breitmeyer, R. E, Johnson, D. J, Ostlund, E. N, MacLachlan, N. J, Wilson, W. C.
      Pages: 553 - 557
      Abstract: Bluetongue is a potentially fatal arboviral disease of domestic and wild ruminants that is characterized by widespread edema and tissue necrosis. Bluetongue virus (BTV) serotypes 10, 11, 13, and 17 occur throughout much of the United States, whereas serotype 2 (BTV-2) was previously only detected in the southeastern United States. Since 1998, 10 other BTV serotypes have also been isolated from ruminants in the southeastern United States. In 2010, BTV-2 was identified in California for the first time, and preliminary sequence analysis indicated that the virus isolate was closely related to BTV strains circulating in the southeastern United States. In the current study, the whole genome sequence of the California strain of BTV-2 was compared with those of other BTV-2 strains in the Americas. The results of the analysis suggest co-circulation of genetically distinct viruses in the southeastern United States, and further suggest that the 2010 western isolate is closely related to southeastern strains of BTV. Although it remains uncertain as to how this novel virus was translocated to California, the findings of the current study underscore the need for ongoing surveillance of this economically important livestock disease.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714536902|hwp:master-id:spvdi;1040638714536902
      Issue No: Vol. 26, No. 4 (2014)
  • Use of an automated system for detection of canine serum antibodies
           against Ehrlichia canis glycoprotein 36
    • Authors: Moroff, S; Sokolchik, I, Woodring, T, Woodruff, C, Atkinson, B, Lappin, M. R.
      Pages: 558 - 562
      Abstract: Ehrlichia canis is the most common cause of monocytotropic ehrlichiosis in dogs around the world. The purpose of the present study was to validate a new automated fluorescence system (Accuplex4™ BioCD system; Antech Diagnostics, Lake Success, New York) to detect antibodies against the E. canis immunodominant glycoprotein 36 (gp36). Sera and blood samples (ethylenediamine tetra-acetic acid) were collected from mixed sex beagles (n = 8) on days 0, 3, 7, 10, 14, 17, 21, 28, 42, 49, 56, 63, 70, 77, 84, and 98 after intravenous inoculation with culture-derived E. canis. Sera were assayed using the Accuplex4 BioCD system (Accuplex4), an E. canis indirect fluorescent antibody test (IFAT), and a commercially available kit. A complete blood cell count and a proprietary E. canis polymerase chain reaction (PCR) were performed on each blood sample. On the day thrombocytopenia was first detected for each dog, E. canis DNA was amplified from blood of all dogs. At those times, E. canis antibodies were detected in 7 of 8 dogs by the Accuplex4, 1 of 8 dogs by the commercial kit, and 4 of 8 dogs by IFAT. Ehrlichia canis DNA was amplified from blood before seroconversion in any antibody assay for 6 dogs. Antibodies against gp36 were detected by Accuplex4 within 3 days of PCR-positive test results and were detected up to 25 days sooner than the commercial kit. After starting doxycycline treatment, E. canis DNA was no longer amplified by PCR assay, but serum antibodies remained detectable by all assays.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714534849|hwp:resource-id:spvdi;26/4/558
      Issue No: Vol. 26, No. 4 (2014)
  • Detection and genetic characterization of Canine parvovirus and Canine
           coronavirus strains circulating in district of Tirana in Albania
    • Authors: Cavalli, A; Desario, C, Kusi, I, Mari, V, Lorusso, E, Cirone, F, Kumbe, I, Colaianni, M. L, Buonavoglia, D, Decaro, N.
      Pages: 563 - 566
      Abstract: An epidemiological survey for Canine parvovirus 2 (CPV-2) and Canine coronavirus (CCoV) was conducted in Albania. A total of 57 fecal samples were collected from diarrheic dogs in the District of Tirana during 2011–2013. The molecular assays detected 53 and 31 CPV- and CCoV-positive specimens, respectively, with mixed CPV–CCoV infections diagnosed in 28 dogs. The most frequently detected CPV type was 2a, whereas IIa was the predominant CCoV subtype. A better comprehension of the CPV–CCoV epidemiology in eastern European countries will help to assess the most appropriate vaccination strategies to prevent disease due to infections with these widespread agents of acute gastroenteritis in the dog.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714538965|hwp:master-id:spvdi;1040638714538965
      Issue No: Vol. 26, No. 4 (2014)
  • Outbreak of Bluetongue virus serotype 4 in dairy sheep in Rio de Janeiro,
    • Authors: Balaro, M. F. A; dos Santos Lima, M, Del Fava, C, de Oliveira, G. R, Pituco, E. M, Brandao, F. Z.
      Pages: 567 - 570
      Abstract: In late January 2013, 10 nonpregnant Lacaune dairy ewes raised under extensive husbandry management on a farm in Rio de Janeiro, Brazil, presented with the general clinical signs of lethargy, hyporexia, edema of the face, hyperemia of the exposed parts of the skin, mouth lesions, pyrexia, and lameness. Additionally, 2 pregnant ewes died suddenly after the onset of respiratory signs. The complete blood counts and biochemistry analyses showed neutrophilic leukocytosis with monocytosis and reactive lymphocytes, normocytic normochromic anemia and increased aspartate aminotransferase levels. Postmortem examination revealed erosions on the lingual mucosa, bilateral submandibular ganglia infarctions, yellow foamy fluid accumulation in the trachea and bronchial bifurcation, pulmonary congestion, and edema associated with hemorrhagic lesions on the pulmonary artery and heart. The clinical and pathological findings were suggestive of bluetongue. For a molecular and virological diagnosis, tissue samples were analyzed by Bluetongue virus–specific real-time reverse transcription polymerase chain reaction (qRT-PCR), and viral isolation was performed in embryonated chicken eggs. For viral typing, positive tissue and egg-isolated samples were analyzed by qRT-PCR using primers and probes specific for the structural VP2 gene in genome segment 2 of all 26 serotypes. There are still no contingency plans for responding to an outbreak of bluetongue disease in Brazil, and this episode emphasizes the need for continuing serological and entomological surveillance programs. Additionally, this report describes the isolation of Bluetongue virus serotype 4 in sheep in the Americas.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714538020|hwp:master-id:spvdi;1040638714538020
      Issue No: Vol. 26, No. 4 (2014)
  • Spontaneous chronic T-cell leukemia in a male rhesus macaque (Macaca
    • Authors: Cazzini, P; Krimer, P. M, Williams-Fritze, M. J, Butler, A. M, Blas-Machado, U.
      Pages: 571 - 574
      Abstract: Blood smears from a 24-year-old male rhesus macaque (Macaca mulatta) used for cognitive function studies were evaluated. The macaque had an 8-month history of gradual weight loss and increasing lymphocytosis. Most of the lymphocytes present were small to medium and had a mature morphology. Based on the degree and duration of the lymphocytosis, and the appearance of the lymphocytes, a diagnosis of chronic lymphocytic leukemia was made. The animal tested negative for 4 viral diseases that are commonly associated with lymphoproliferative disorders in Old World monkeys. Over the course of 12 months, the lymphocytosis progressed from 18.4 to 384 x 103 lymphocytes/µl (reference range: 0.8–17 x 103 cells/µl), and euthanasia was elected. On histologic examination, cluster of differentiation (CD)3- and CD8-positive, CD79-negative neoplastic cells comprised 40–60% of the bone marrow, diffusely obscured the normal splenic architecture, and were present in the vascular channels in other organs. Findings were characteristic of T-cell lymphocytic leukemia. Naturally occurring T-cell lymphocytic leukemia has been rarely reported in rhesus macaques and, to the authors’ knowledge, never in males. A persistent lymphocytosis characterized by a monomorphic population of CD3- and CD8-positive cytotoxic T-lymphocytes and the presence of neoplastic cells in the bone marrow led to a diagnosis in the current case.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714532339|hwp:master-id:spvdi;1040638714532339
      Issue No: Vol. 26, No. 4 (2014)
  • Periocular extracranial cutaneous meningiomas in two dogs
    • Authors: Teixeira, L. B. C; Pinkerton, M. E, Dubielzig, R. R.
      Pages: 575 - 579
      Abstract: Cutaneous meningiomas are rare tumors in human beings and animals. Two canine cases of cutaneous meningiomas affecting the eyelid are described in the current study: the first from a 5-week-old female Springer Spaniel dog with an 8 cm in diameter congenital mass expanding the left upper eyelid and medial canthus; the second from a 10-year-old female spayed Maltese–Poodle mix dog with 3 firm subcutaneous nodules affecting the right upper eyelid. All masses were removed surgically. Histologically, tumors were composed of spindle-to-epithelioid cells arranged in small lobules forming solid concentric whorls. Neoplastic cells were positive for vimentin and S100 and negative for pancytokeratin, glial fibrillar acid protein, and neurofilament. Transmission electron microscopy revealed meningothelial cells with convoluted interdigitating processes, desmosomes, and hemidesmosomes, and moderate numbers of cytoplasmic microfilaments. None of the cases presented a primary neuroaxial meningioma. The first case presents clinicopathological features consistent with human type I (congenital) cutaneous meningioma. The second case is consistent with a type II (acquired ectopic) tumor, and both are hypothesized to arise from ectopic arachnoid cells displaced during development.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714533116|hwp:master-id:spvdi;1040638714533116
      Issue No: Vol. 26, No. 4 (2014)
  • Nocardia cyriacigeorgica as the causative agent of mandibular
           osteomyelitis (lumpy jaw) in a cat
    • Authors: Soto, E; Arauz, M, Gallagher, C. A, Illanes, O.
      Pages: 580 - 584
      Abstract: An unusual case of osteomyelitis caused by Nocardia cyriacigeorgica infection and resulting in mandibular osteomyelitis and cellulitis (lumpy jaw) is described in a young cat. A 1-cm hard nodular mass was an incidental finding in the right mandible of a 14-month-old cat during routine physical examination. The lesion was fast growing, reaching up to 6 cm in its largest dimension over a 5-week period. A core biopsy of the affected mandible revealed foci of osteolysis, woven bone formation, and a few large clusters of filamentous bacteria surrounded by fine eosinophilic amorphous material bordered by neutrophils, plasma cells, macrophages, and occasional multinucleated giant cells. Pure cultures of acid-fast variable, Gram-positive filamentous bacteria were recovered on blood and chocolate agar plates at 48-hr postinoculation. On amplification and sequencing of the 16S ribosomal RNA and 65-kDa heat shock protein genes, the microorganisms were identified as N. cyriacigeorgica, within the actinomycetes.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714533117|hwp:master-id:spvdi;1040638714533117
      Issue No: Vol. 26, No. 4 (2014)
  • Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis
    • Authors: Liu, H; Yan, J, Wang, Y, Yan, Q, Zhao, L, Yan, R, He, H.
      Pages: 585 - 587
      Abstract: Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive. The sequence of the 16S ribosomal DNA (rDNA) gene of the isolates matched the 16S rDNA sequence of M. bovoculi. Amplification of the 16S–23S intergenic spacer region followed by AfaI digestion produced a 600–base pair product, a result characteristic of M. bovoculi isolates. The phylogenetic analysis based on the 16S rDNA sequence confirmed the strain isolated in the current study had genetic homology with M. bovoculi.
      PubDate: 2014-07-15T09:41:02-07:00
      DOI: 10.1177/1040638714535601|hwp:master-id:spvdi;1040638714535601
      Issue No: Vol. 26, No. 4 (2014)
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