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  Subjects -> VETERINARY SCIENCE (Total: 216 journals)
Showing 1 - 63 of 63 Journals sorted alphabetically
Acta Scientiae Veterinariae     Open Access   (Followers: 1)
Acta Veterinaria     Open Access   (Followers: 1)
Acta Veterinaria Brasilica     Open Access  
Acta Veterinaria Hungarica     Full-text available via subscription   (Followers: 2)
Acta Veterinaria Scandinavica     Open Access   (Followers: 1)
Advances in Small Animal Medicine and Surgery     Hybrid Journal  
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 14)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 12)
African Journal of Wildlife Research     Full-text available via subscription   (Followers: 1)
Alexandria Journal of Veterinary Sciences     Open Access  
American Journal of Animal and Veterinary Sciences     Open Access   (Followers: 9)
American Journal of Primatology     Hybrid Journal   (Followers: 13)
American Journal of Veterinary Research     Full-text available via subscription   (Followers: 21)
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C     Hybrid Journal   (Followers: 3)
Animal Behaviour     Hybrid Journal   (Followers: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5)
Animal Nutrition     Open Access   (Followers: 8)
Animal Reproduction     Open Access   (Followers: 1)
Animal Reproduction Science     Hybrid Journal   (Followers: 4)
Animals     Open Access   (Followers: 6)
Annals of Agricultural and Environmental Medicine     Open Access   (Followers: 1)
Annual Review of Animal Biosciences     Full-text available via subscription   (Followers: 4)
Anthrozoos : A Multidisciplinary Journal of The Interactions of People & Animals     Hybrid Journal   (Followers: 7)
Archives of Animal Nutrition     Hybrid Journal   (Followers: 7)
Archivos de Medicina Veterinaria     Open Access   (Followers: 1)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access  
Arquivos de Ciências Veterinárias e Zoologia da UNIPAR     Open Access  
Ars Veterinaria     Open Access  
Asian Journal of Medical and Biological Research     Open Access   (Followers: 1)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atatürk Üniversitesi Veteriner Bilimleri Dergisi     Open Access  
Australian Equine Veterinarian     Full-text available via subscription   (Followers: 3)
Australian Veterinary Journal     Hybrid Journal   (Followers: 16)
Avances en Ciencias Veterinarias     Open Access  
Avian Diseases     Full-text available via subscription   (Followers: 5)
Avian Pathology     Hybrid Journal   (Followers: 2)
Bangladesh Journal of Animal Science     Open Access   (Followers: 1)
Bangladesh Journal of Veterinary Medicine     Open Access   (Followers: 2)
Bangladesh Veterinarian     Open Access  
BMC Veterinary Research     Open Access   (Followers: 11)
Brazilian Journal of Veterinary Research and Animal Science     Open Access   (Followers: 8)
Buletin Peternakan : Bulletin of Animal Science     Full-text available via subscription  
Buletin Veteriner Udayana     Open Access   (Followers: 2)
Bulletin of Animal Health and Production in Africa     Full-text available via subscription   (Followers: 1)
Bulletin of the Veterinary Institute in Pulawy     Open Access  
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access  
Canadian Journal of Veterinary Research     Full-text available via subscription   (Followers: 8)
Case Reports in Veterinary Medicine     Open Access   (Followers: 5)
Ciência Animal Brasileira     Open Access  
Ciência Rural     Open Access   (Followers: 2)
Cogent Food & Agriculture     Open Access   (Followers: 1)
Companion Animal     Full-text available via subscription   (Followers: 9)
Domestic Animal Endocrinology     Hybrid Journal   (Followers: 5)
Equine Health     Full-text available via subscription   (Followers: 3)
Equine Veterinary Education     Hybrid Journal   (Followers: 9)
Equine Veterinary Journal     Hybrid Journal   (Followers: 16)
Ethiopian Veterinary Journal     Open Access   (Followers: 5)
Eurasian Journal of Veterinary Sciences     Open Access   (Followers: 2)
FAVE Sección Ciencias Veterinarias     Open Access  
Folia Veterinaria     Open Access  
Frontiers in Veterinary Science     Open Access   (Followers: 1)
Global Journal of Animal Scientific Research     Open Access   (Followers: 1)
Human & Veterinary Medicine - International Journal of the Bioflux Society     Open Access   (Followers: 6)
ILAR Journal     Hybrid Journal   (Followers: 1)
In Practice     Full-text available via subscription   (Followers: 2)
Indian Journal of Animal Sciences     Open Access   (Followers: 6)
Indian Journal of Veterinary Anatomy     Full-text available via subscription   (Followers: 4)
Indonesia Medicus Veterinus     Open Access  
Intas Polivet     Full-text available via subscription   (Followers: 1)
International Journal for Agro Veterinary and Medical Sciences     Open Access   (Followers: 2)
International Journal of Livestock Research     Open Access   (Followers: 2)
International Journal of Veterinary Science and Medicine     Open Access   (Followers: 4)
Iranian Journal of Applied Animal Science     Open Access   (Followers: 1)
Irish Veterinary Journal     Open Access   (Followers: 5)
İstanbul Üniversitesi Veteriner Fakültesi Dergisi     Open Access  
Journal of Veterinary Science & Technology     Open Access   (Followers: 7)
Journal of Advanced Veterinary and Animal Research     Open Access   (Followers: 5)
Journal of Animal Behaviour and Biometeorology     Open Access   (Followers: 2)
Journal of Animal Physiology and Animal Nutrition     Hybrid Journal   (Followers: 5)
Journal of Animal Science and Technology     Open Access  
Journal of Avian Medicine and Surgery     Full-text available via subscription   (Followers: 5)
Journal of Buffalo Science     Hybrid Journal  
Journal of Equine Veterinary Science     Hybrid Journal   (Followers: 12)
Journal of Exotic Pet Medicine     Full-text available via subscription   (Followers: 3)
Journal of Experimental and Applied Animal Sciences     Open Access   (Followers: 1)
Journal of Feline Medicine & Surgery     Hybrid Journal   (Followers: 4)
Journal of Feline Medicine and Surgery Open Reports     Open Access  
Journal of Research in Forestry, Wildlife and Environment     Open Access   (Followers: 4)
Journal of Small Animal Practice     Hybrid Journal   (Followers: 10)
Journal of the American Veterinary Medical Association     Full-text available via subscription   (Followers: 31)
Journal of the Selva Andina Research Society     Open Access   (Followers: 1)
Journal of the South African Veterinary Association     Open Access   (Followers: 2)
Journal of Venomous Animals and Toxins     Open Access   (Followers: 4)
Journal of Veterinary Advances     Open Access   (Followers: 4)
Journal of Veterinary Behavior: Clinical Applications and Research     Hybrid Journal   (Followers: 4)
Journal of Veterinary Cardiology     Full-text available via subscription   (Followers: 7)
Journal of Veterinary Diagnostic Investigation     Hybrid Journal   (Followers: 8)
Journal of Veterinary Emergency and Critical Care     Hybrid Journal   (Followers: 16)
Journal of Veterinary Internal Medicine     Open Access   (Followers: 22)
Journal of Veterinary Medical Education     Partially Free   (Followers: 12)
Journal of Veterinary Medicine     Open Access   (Followers: 9)
Journal of Veterinary Medicine and Animal Health     Open Access   (Followers: 5)
Journal of Veterinary Pharmacology and Therapeutics     Hybrid Journal   (Followers: 8)
Journal of Veterinary Research     Open Access   (Followers: 1)
Journal of Veterinary Science & Medical Diagnosis     Hybrid Journal   (Followers: 4)
Journal of Zoo and Aquarium Research     Open Access   (Followers: 4)
Journal of Zoo and Wildlife Medicine     Full-text available via subscription   (Followers: 6)
Jurnal Agripet     Open Access  
Jurnal Medika Veterinaria     Open Access  
Kenya Veterinarian     Full-text available via subscription   (Followers: 2)
kleintier konkret     Hybrid Journal  
Livestock     Full-text available via subscription   (Followers: 2)
Macedonian Veterinary Review     Open Access   (Followers: 2)
Media Peternakan - Journal of Animal Science and Technology     Open Access   (Followers: 1)
Medical Mycology     Open Access   (Followers: 4)
Medical Mycology Case Reports     Open Access   (Followers: 1)
Microbes and Health     Open Access   (Followers: 1)
New Zealand Veterinary Journal     Full-text available via subscription   (Followers: 11)
New Zealand Veterinary Nurse     Full-text available via subscription   (Followers: 4)
Nigerian Veterinary Journal     Open Access  
Nutrición Animal Tropical     Open Access   (Followers: 1)
Onderstepoort Journal of Veterinary Research     Open Access   (Followers: 3)
Open Access Animal Physiology     Open Access   (Followers: 4)
Open Journal of Animal Sciences     Open Access   (Followers: 4)
Open Journal of Veterinary Medicine     Open Access   (Followers: 4)
Pesquisa Veterinária Brasileira     Open Access  
pferde spiegel     Hybrid Journal  
Polish Journal of Veterinary Sciences     Open Access   (Followers: 3)
Pratique Médicale et Chirurgicale de l'Animal de Compagnie     Full-text available via subscription  
Preventive Veterinary Medicine     Hybrid Journal   (Followers: 10)
REDVET. Revista Electrónica de Veterinaria     Open Access  
Reproduction in Domestic Animals     Hybrid Journal   (Followers: 1)
Research & Reviews : Journal of Veterinary Science and Technology     Full-text available via subscription   (Followers: 1)
Research in Veterinary Science     Hybrid Journal   (Followers: 10)
Research Journal of Veterinary Sciences     Open Access   (Followers: 10)
Revista Brasileira de Ciência Veterinária     Open Access  
Revista Brasileira de Higiene e Sanidade Animal     Open Access  
Revista Brasileira de Parasitologia Veterinaria     Open Access  
Revista Brasileira de Reprodução Animal     Open Access  
Revista Brasileira de Zootecnia     Open Access   (Followers: 2)
Revista Ciencia Animal     Open Access  
Revista Ciencias Veterinarias     Open Access  
Revista Científica     Open Access  
Revista Colombiana de Ciencias Pecuarias (Colombian journal of animal science and veterinary medicine)     Open Access   (Followers: 1)
Revista Complutense de Ciencias Veterinarias     Open Access  
Revista da Sociedade Brasileira de Ciência em Animais de Laboratório     Open Access  
Revista de Ciências Agroveterinárias     Open Access  
Revista de Educação Continuada em Medicina Veterinária e Zootecnia     Open Access  
Revista de Investigaciones Veterinarias del Perú     Open Access  
Revista de la Facultad de Medicina Veterinaria y de Zootecnia     Open Access   (Followers: 1)
Revista de Medicina Veterinaria     Open Access  
Revista de Salud Animal     Open Access  
Revista Mexicana de Ciencias Pecuarias     Open Access  
Revista MVZ Córdoba     Open Access  
Revista Veterinaria     Open Access  
Revue Marocaine des Sciences Agronomiques et Vétérinaires     Open Access  
Revue Vétérinaire Clinique     Full-text available via subscription  
SA Stud Breeder / SA Stoetteler     Full-text available via subscription  
Schweizer Archiv für Tierheilkunde     Hybrid Journal  
Scientific Journal of Animal Science     Open Access   (Followers: 3)
Scientific Journal of Veterinary Advances     Open Access   (Followers: 1)
Small Ruminant Research     Hybrid Journal   (Followers: 2)
Sokoto Journal of Veterinary Sciences     Open Access   (Followers: 1)
Spei Domus     Open Access  
Tanzania Veterinary Journal     Full-text available via subscription   (Followers: 1)
team.konkret     Open Access  
The Dairy Mail     Full-text available via subscription   (Followers: 3)
Theriogenology     Hybrid Journal   (Followers: 1)
Topics in Companion Animal Medicine     Hybrid Journal   (Followers: 3)
Transboundary and Emerging Diseases     Hybrid Journal   (Followers: 2)
Trends in Animal and Veterinary Sciences     Open Access   (Followers: 6)
Trends in Parasitology     Full-text available via subscription   (Followers: 8)
Tropical Animal Health and Production     Hybrid Journal  
Tropical Veterinarian     Full-text available via subscription   (Followers: 1)
Turkish Journal of Veterinary and Animal Sciences     Open Access  
veterinär spiegel     Hybrid Journal   (Followers: 1)
Veterinária e Zootecnia     Open Access  
Veterinária em Foco     Open Access  
Veterinaria México     Open Access  
Veterinária Notícias     Open Access  
Veterinary Anaesthesia and Analgesia     Hybrid Journal   (Followers: 11)
Veterinary and Comparative Oncology     Hybrid Journal   (Followers: 7)
Veterinary Clinical Pathology     Hybrid Journal   (Followers: 9)
Veterinary Clinics of North America: Equine Practice     Full-text available via subscription   (Followers: 10)
Veterinary Clinics of North America: Exotic Animal Practice     Full-text available via subscription   (Followers: 7)
Veterinary Clinics of North America: Food Animal Practice     Full-text available via subscription   (Followers: 5)
Veterinary Clinics of North America: Small Animal Practice     Full-text available via subscription   (Followers: 16)
Veterinary Dermatology     Hybrid Journal   (Followers: 7)
Veterinary Immunology and Immunopathology     Hybrid Journal   (Followers: 9)
Veterinary Journal     Hybrid Journal   (Followers: 16)
Veterinary Medicine and Animal Sciences     Open Access   (Followers: 4)
Veterinary Medicine and Science     Open Access   (Followers: 1)
Veterinary Medicine International     Open Access   (Followers: 8)
Veterinary Medicine: Research and Reports     Open Access   (Followers: 4)
Veterinary Microbiology     Hybrid Journal   (Followers: 8)
Veterinary Nurse     Full-text available via subscription   (Followers: 5)
Veterinary Nursing Journal     Hybrid Journal   (Followers: 4)
Veterinary Ophthalmology     Hybrid Journal   (Followers: 6)
Veterinary Parasitology     Hybrid Journal   (Followers: 9)
Veterinary Parasitology : Regional Studies and Reports     Full-text available via subscription  

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Journal Cover Journal of Veterinary Diagnostic Investigation
  [SJR: 0.701]   [H-I: 60]   [8 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1040-6387 - ISSN (Online) 1943-4936
   Published by Sage Publications Homepage  [853 journals]
  • Letter to the Editor
    • Authors: Behr, M; Rudd, R, Driemeier, D.
      Pages: 621 - 622
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716675144
      Issue No: Vol. 28, No. 6 (2016)
       
  • Unclassified sarcomas: a study to improve classification in a cohort of
           Golden Retriever dogs
    • Authors: Boerkamp, K. M; Hellmen, E, Willen, H, Grinwis, G. C. M, Teske, E, Rutteman, G. R.
      Pages: 623 - 631
      Abstract: Morphologically, canine soft-tissue sarcomas (STSs) resemble human STSs. In humans, proper classification of STSs is considered essential to improve insight in the biology of these tumors, and to optimize diagnosis and therapy. To date, there is a paucity of data published on the significance of detailed classification of STSs in the dog. We revised a cohort (n = 110) of proliferative lesions obtained from a study in Golden Retrievers that were considered "soft tissue sarcoma, not otherwise specified or of uncertain subtype" in order to optimize the diagnoses of these lesions. The criteria according to the veterinary WHO classification, recent veterinary literature, and the WHO classification for humans were applied. Revision was initially based on morphologic characteristics of hematoxylin and eosin–stained histologic sections of the neoplasms. If considered necessary (n = 76), additional immunohistochemistry was applied to aid characterization. The diagnosis of STS was confirmed in 75 neoplasms (68%). Of this group, diagnosis of a specific subtype of the STSs was possible in 58 neoplasms. Seven neoplasms had morphologic characteristics that were suggestive for sarcoma subtypes only described in the WHO classification for humans. Seventeen neoplasms remained "unclassified STSs." Thirty-one lesions (28%) were diagnosed "neoplasm, not being STS." Four lesions (4%) were considered nonneoplastic. Because incorrect classification of a tumor could lead to inappropriate therapeutic intervention and prognostication, the results of our study clearly illustrate the importance of revision and further diagnosis of "unclassified STSs" in dogs.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716660130
      Issue No: Vol. 28, No. 6 (2016)
       
  • Nucleotide sequence analysis of a DNA region involved in capsular
           polysaccharide biosynthesis reveals the molecular basis of the
           nontypeability of two Actinobacillus pleuropneumoniae isolates
    • Authors: Ito, H; Ogawa, T, Fukamizu, D, Morinaga, Y, Kusumoto, M.
      Pages: 632 - 637
      Abstract: The aim of our study was to reveal the molecular basis of the serologic nontypeability of 2 Actinobacillus pleuropneumoniae field isolates. Nine field strains of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia, were isolated from pigs raised on the same farm and sent to our diagnostic laboratory for serotyping. Seven of the 9 strains were identified as serovar 15 strains by immunodiffusion tests. However, 2 strains, designated FH24-2 and FH24-5, could not be serotyped with antiserum prepared against serovars 1–15. Strain FH24-5 showed positive results in 2 serovar 15–specific PCR tests, whereas strain FH24-2 was only positive in 1 of the 2 PCR tests. The nucleotide sequence analysis of gene clusters involved in capsular polysaccharide biosynthesis of the 2 nontypeable strains revealed that both had been rendered nontypeable by the action of ISApl1, a transposable element of A. pleuropneumoniae belonging to the IS30 family. The results showed that ISApl1 of A. pleuropneumoniae can interfere with both the serologic and molecular typing methods, and that nucleotide sequence analysis across the capsular gene clusters is the best means of determining the cause of serologic nontypeability in A. pleuropneumoniae.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716656026
      Issue No: Vol. 28, No. 6 (2016)
       
  • Evaluation of a PCR assay on overgrown environmental samples cultured for
           Mycobacterium avium subsp. paratuberculosis
    • Authors: Arango-Sabogal, J. C; Labrecque, O, Pare, J, Fairbrother, J.-H, Roy, J.-P, Wellemans, V, Fecteau, G.
      Pages: 638 - 645
      Abstract: Culture of Mycobacterium avium subsp. paratuberculosis (MAP) is the definitive antemortem test method for paratuberculosis. Microbial overgrowth is a challenge for MAP culture, as it complicates, delays, and increases the cost of the process. Additionally, herd status determination is impeded when noninterpretable (NI) results are obtained. The performance of PCR is comparable to fecal culture, thus it may be a complementary detection tool to classify NI samples. Our study aimed to determine if MAP DNA can be identified by PCR performed on NI environmental samples and to evaluate the performance of PCR before and after the culture of these samples in liquid media. A total of 154 environmental samples (62 NI, 62 negative, and 30 positive) were analyzed by PCR before being incubated in an automated system. Growth was confirmed by acid-fast bacilli stain and then the same PCR method was again applied on incubated samples, regardless of culture and stain results. Change in MAP DNA after incubation was assessed by converting the PCR quantification cycle (Cq) values into fold change using the 2–Cq method (Cq = Cq after culture – Cq before culture). A total of 1.6% (standard error [SE] = 1.6) of the NI environmental samples had detectable MAP DNA. The PCR had a significantly better performance when applied after culture than before culture (p = 0.004). After culture, a 66-fold change (SE = 17.1) in MAP DNA was observed on average. Performing a PCR on NI samples improves MAP culturing. The PCR method used in our study is a reliable and consistent method to classify NI environmental samples.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716662302
      Issue No: Vol. 28, No. 6 (2016)
       
  • Intralaboratory development and evaluation of a high-performance liquid
           chromatography-fluorescence method for detection and quantitation of
           aflatoxins M1, B1, B2, G1, and G2 in animal liver
    • Authors: Shao, D; Imerman, P. M, Schrunk, D. E, Ensley, S. M, Rumbeiha, W. K.
      Pages: 646 - 655
      Abstract: Aflatoxins are potent mycotoxins with effects that include hepatotoxicity, immunosuppression, and suppression of animal growth and production. The etiologic diagnosis of aflatoxicosis, which is largely based on analysis of contaminated feed matrices, has significant disadvantages given the fact that representative feed samples may not be available and feed-based test methods are not confirmatory of an etiologic diagnosis. A tissue-based analytical method for biomarkers of exposure would be valuable for confirmation of aflatoxicosis. We describe in-house development and evaluation of a high-performance liquid chromatographic method with fluorescence detection and precolumn derivatization for determination of aflatoxins M1, B1, B2, G1, and G2 in animal liver. The method demonstrates good selectivity for the tested aflatoxins in the liver matrix. The overall range was 0.03–0.10 ng/g for limit of detection and 0.09–0.18 ng/g for limit of quantitation. The correlation coefficient (R2) of calibration curves was >0.9978 for AFM1, 0.9995 for AFB1, 0.9986 for AFB2, 0.9983 for AFG1, and 0.9980 for AFG2. For fortification levels of 0.2–10 ng/g, repeatability was 10–18% for AFM1, 7–14% for AFB1, 5–14% for AFB2, 6–16% for AFG1, and 10–15% for AFG2. Recovery was 52–57% for AFM1, 54–62% for AFB1, 55–61% for AFB2, 57–67% for AFG1, and 61–65% for AFG2. There was no liver matrix effect found. The method is rugged against minor changes based on the selected factors. The results indicate that the proposed method is suitable for quantitative determination of aflatoxins M1, B1, B2, G1, and G2 in liver.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716668217
      Issue No: Vol. 28, No. 6 (2016)
       
  • Spontaneous reproductive pathology in female guinea pigs
    • Authors: Veiga-Parga, T; La Perle, K. M. D, Newman, S. J.
      Pages: 656 - 661
      Abstract: Reproductive pathology of domestic guinea pigs is underreported to date. To provide a comprehensive review of uterine disease in guinea pigs, we performed a retrospective study of the pathology archives of the University of Tennessee, College of Veterinary Medicine. By histology, 13 of 37 uterine lesions in 23 animals were neoplastic; the other 24 nonneoplastic lesions included cystic endometrial hyperplasia (16 of 24), endometrial hemorrhage (3 of 24), pyometra (2 of 24), polyp (2 of 24), and mucometra (1 of 24). The most common guinea pig uterine neoplasms were uterine leiomyomas (6 of 13), followed by adenomas (3 of 13) and leiomyosarcomas (1 of 13). Other neoplasms included anaplastic tumors of unknown origin (2 of 13) and choriocarcinoma (1 of 13). Both anaplastic tumors and the choriocarcinoma were positive for vimentin. The choriocarcinoma was positive for HSD83B1, indicating a trophoblastic origin and its final diagnosis. All were negative for cytokeratin and smooth muscle. In multiple animals, more than 1 tumor or lesion was reported. Estrogen receptor and progesterone receptor expression was nearly 100% in uterine neoplasms. Nearly all animals for which data were available had cystic rete ovarii (18 of 19); the animal with no cystic rete ovarii had paraovarian cysts. In our study, female pet guinea pigs had a tendency to develop cystic endometrial hyperplasia and uterine neoplasia. Factors for the development of these lesions could be cystic rete ovarii, hormone dysregulation, and/or age. Other factors could contribute to the development of uterine lesions. As in other species, early ovariohysterectomy could decrease the prevalence of uterine lesions.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716665429
      Issue No: Vol. 28, No. 6 (2016)
       
  • Neoplasia in 125 donkeys (Equus asinus): literature review and a survey of
           five veterinary schools in the United States and Canada
    • Authors: Davis, C. R; Valentine, B. A, Gordon, E, McDonough, S. P, Schaffer, P. A, Allen, A. L, Pesavento, P.
      Pages: 662 - 670
      Abstract: A diagnosis of neoplasia was noted in 125 of 357 donkeys (35%) in our review of medical records from 5 veterinary schools in the United States and Canada. Equine sarcoid was the most common tumor in our study, accounting for 72% of all tumors and 82% of cutaneous tumors. Soft-tissue sarcomas were the second most common skin tumors. All other types of neoplasia were rare. Important differences in the occurrence of neoplasia in donkeys compared to horses included the rarity or absence of squamous cell carcinoma in any organ system and gray horse melanoma. Lymphosarcoma, the most common malignant tumor in horses, appears to be very rare in donkeys. We report several tumors in donkeys including melanocytoma, peripheral nerve sheath tumor, and gastrointestinal stromal tumor. Our data demonstrate commonalities as well as differences in neoplastic diseases of donkeys and horses. Understanding differences in carcinogenesis among these 2 closely related species can inform researchers pursuing pathogenic mechanisms of equine disease and inform veterinary diagnosticians regarding tumor prevalence.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716665659
      Issue No: Vol. 28, No. 6 (2016)
       
  • Tissue localization, shedding, virus carriage, antibody response, and
           aerosol transmission of Porcine epidemic diarrhea virus following
           inoculation of 4-week-old feeder pigs
    • Authors: Niederwerder, M. C; Nietfeld, J. C, Bai, J, Peddireddi, L, Breazeale, B, Anderson, J, Kerrigan, M. A, An, B, Oberst, R. D, Crawford, K, Lager, K. M, Madson, D. M, Rowland, R. R. R, Anderson, G. A, Hesse, R. A.
      Pages: 671 - 678
      Abstract: We determined tissue localization, shedding patterns, virus carriage, antibody response, and aerosol transmission of Porcine epidemic diarrhea virus (PEDV) following inoculation of 4-week-old feeder pigs. Thirty-three pigs were randomly assigned to 1 of 3 groups for the 42-day study: inoculated (group A; n = 23), contact transmission (group B; n = 5), and aerosol transmission (group C; n = 5). Contact transmission occurred rapidly to group B pigs whereas productive aerosol transmission failed to occur to group C pigs. Emesis was the first clinical sign noted at 3 days postinoculation (dpi) followed by mild to moderate diarrhea lasting 5 more days. Real-time PCR detected PEDV in fecal and nasal swabs, oral fluids, serum, and gastrointestinal and lymphoid tissues. Shedding occurred primarily during the first 2 weeks postinoculation, peaking at 5–6 dpi; however, some pigs had PEDV nucleic acid detected in swabs collected at 21 and 28 dpi. Antibody titers were measurable between 14 and 42 dpi. Although feces and intestines collected at 42 dpi were PEDV negative by PCR and immunohistochemistry, respectively, small intestines from 70% of group A pigs were PCR positive. Although disease was relatively mild and transient in this age group, the results demonstrate that 4-week-old pigs are productively infected and can sustain virus replication for several weeks. Long-term shedding of PEDV in subclinically affected pigs should be considered an important source for PEDV transmission.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716663251
      Issue No: Vol. 28, No. 6 (2016)
       
  • Evaluation of supervised machine-learning algorithms to distinguish
           between inflammatory bowel disease and alimentary lymphoma in cats
    • Authors: Awaysheh, A; Wilcke, J, Elvinger, F, Rees, L, Fan, W, Zimmerman, K. L.
      Pages: 679 - 687
      Abstract: Inflammatory bowel disease (IBD) and alimentary lymphoma (ALA) are common gastrointestinal diseases in cats. The very similar clinical signs and histopathologic features of these diseases make the distinction between them diagnostically challenging. We tested the use of supervised machine-learning algorithms to differentiate between the 2 diseases using data generated from noninvasive diagnostic tests. Three prediction models were developed using 3 machine-learning algorithms: naive Bayes, decision trees, and artificial neural networks. The models were trained and tested on data from complete blood count (CBC) and serum chemistry (SC) results for the following 3 groups of client-owned cats: normal, inflammatory bowel disease (IBD), or alimentary lymphoma (ALA). Naive Bayes and artificial neural networks achieved higher classification accuracy (sensitivities of 70.8% and 69.2%, respectively) than the decision tree algorithm (63%, p < 0.0001). The areas under the receiver-operating characteristic curve for classifying cases into the 3 categories was 83% by naive Bayes, 79% by decision tree, and 82% by artificial neural networks. Prediction models using machine learning provided a method for distinguishing between ALA–IBD, ALA–normal, and IBD–normal. The naive Bayes and artificial neural networks classifiers used 10 and 4 of the CBC and SC variables, respectively, to outperform the C4.5 decision tree, which used 5 CBC and SC variables in classifying cats into the 3 classes. These models can provide another noninvasive diagnostic tool to assist clinicians with differentiating between IBD and ALA, and between diseased and nondiseased cats.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716657377
      Issue No: Vol. 28, No. 6 (2016)
       
  • Identification of Fusarium solani species complex from infected zebrafish
           (Danio rerio)
    • Authors: Ke, X; Lu, M, Wang, J.
      Pages: 688 - 692
      Abstract: Although Fusarium sp. infections have been reported in aquatic invertebrates, studies of Fusarium spp. as fish pathogens remain very limited. In our study, a fungus was isolated from diseased zebrafish (Danio rerio). DNA sequence analysis of the fungus, based on a partial region of the translation elongation factor 1α gene (EF-1α), the internal transcribed spacer region and domains D1 and D2 of the large subunit of the ribosomal RNA gene (ITS plus LSU), and the RNA polymerase II subunit gene (RPB2), showed 99.9–100% homology to Fusarium solani species complex sequences. Multilocus sequence typing analysis based on 3-locus haplotypes (EF-1α, ITS plus LSU, and RPB2) suggests that the isolated strain was type 3+4-P. Challenge experiments showed that this organism could be pathogenic to zebrafish, but usually does not infect healthy subjects under normal circumstances.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716669539
      Issue No: Vol. 28, No. 6 (2016)
       
  • Validation of three automated assays for total antioxidant capacity
           determination in canine serum samples
    • Authors: Rubio, C. P; Hernandez-Ruiz, J, Martinez-Subiela, S, Tvarijonaviciute, A, Arnao, M. B, Ceron, J. J.
      Pages: 693 - 698
      Abstract: We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716664939
      Issue No: Vol. 28, No. 6 (2016)
       
  • Biological variation of 20 analytes measured in serum from clinically
           healthy domestic cats
    • Authors: Falkenö, U; Hillström, A, von Brömssen, C, Strage, E. M.
      Pages: 699 - 704
      Abstract: The applications of data on biological variation include assessment of the utility of population-based reference intervals, evaluation of the significance of change in serial results, and setting of analytical quality specifications. We investigated the biological variation of 19 biochemistry analytes and total T4, measured in serum from 7 clinically healthy domestic cats sampled once weekly for 5 weeks. Samples were frozen and analyzed in random order in the same analytical run. Results were analyzed for outliers, and the components of variance, subsequently generated by restricted maximum likelihood, were used to determine within-subject and between-subject variation (CVI and CVG, respectively), as well as analytical variation (CVA) for each analyte. Indices of individuality, reference change values, and analytical performance goals were calculated. The smallest CVI and CVG were found for calcium, chloride, and sodium, whereas the largest values were calculated for bile acids. Nine analytes (albumin, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, cholesterol, creatinine, phosphate [phosphorus], total protein, total T4) demonstrated high individuality, indicating limited utility of population-based reference intervals. Individuality was low, and population-based reference intervals were thereby considered appropriate for 5 analytes (bile acids, calcium, fructosamine, glucose, potassium). The intermediate individuality observed for 4 analytes (creatine kinase, iron, magnesium, urea) indicated that population-based reference intervals should be used with caution.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716666602
      Issue No: Vol. 28, No. 6 (2016)
       
  • Activity, specificity, and titer of naturally occurring canine anti-DEA 7
           antibodies
    • Authors: Spada, E; Proverbio, D, Baggiani, L, Canzi, I, Perego, R.
      Pages: 705 - 708
      Abstract: The reported prevalence of naturally occurring anti–dog erythrocyte antigen (DEA) 7 antibodies in DEA 7–negative dogs is as high as 50%. Characterization of these antibodies may better define their importance in canine transfusion medicine. We determined in vitro activity, specificity, and titer of anti–DEA 7 antibodies in DEA 7–negative dogs. Plasma samples from 317 DEA 7–negative dogs were cross-matched with DEA 7–positive red blood cells (RBCs) using gel column technology. Agglutination occurred with DEA 7–positive RBCs but not with DEA 7–negative RBCs in 73 samples (23%), which were hence classified as containing anti–DEA 7 antibodies. These samples were evaluated for hemolytic and agglutinating activity, strength of agglutination, and antibody specificity and titers. All samples showed agglutination but none showed hemolysis. Gel agglutination was graded as 1+ for 20 samples (27%), 2+ for 49 samples (67%), 3+ for 4 samples (6%); no samples were graded 4+. The agglutination titer was
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716668626
      Issue No: Vol. 28, No. 6 (2016)
       
  • Snake fungal disease caused by Ophidiomyces ophiodiicola in a free-ranging
           mud snake (Farancia abacura)
    • Authors: Last, L. A; Fenton, H, Gonyor-McGuire, J, Moore, M, Yabsley, M. J.
      Pages: 709 - 713
      Abstract: Snake fungal disease is an emerging infectious disease caused by the fungus Ophidiomyces ophiodiicola leading to severe dermatitis and facial disfiguration in numerous free-ranging and captive snakes. A free-ranging mud snake (Farancia abacura) from Bulloch County, Georgia, was presented for autopsy because of facial swelling and emaciation. Extensive ulceration of the skin, which was especially severe on the head, and retained shed were noted on external examination. Microscopic examination revealed severe heterophilic dermatitis with intralesional fungal hyphae and arthroconidia consistent with O. ophiodiicola. A skin sample incubated on Sabouraud dextrose agar yielded a white-to-tan powdery fungal culture that was confirmed to be O. ophiodiicola by polymerase chain reaction and sequence analysis. Heavy infestation with adult tapeworms (Ophiotaenia faranciae) was present within the intestine. Various bacterial and fungal species, interpreted to either be secondary invaders or postmortem contaminants, were associated with oral lesions. Although the role of these other organisms in the overall health of this individual is not known, factors such as concurrent infections or immunosuppression should be considered in order to better understand the overall manifestation of snake fungal disease, which remains poorly characterized in its host range and geographic distribution.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716663250
      Issue No: Vol. 28, No. 6 (2016)
       
  • Evaluation and improvement of a single nucleotide polymorphism-based PCR
           assay for rapid differentiation of live attenuated vaccine strains from
           field isolates of Erysipelothrix rhusiopathiae
    • Authors: Zhu, W; Li, J, Wang, Y, Kang, C, Jin, M, Chen, H.
      Pages: 714 - 717
      Abstract: A single nucleotide polymorphism–based PCR assay has been developed to differentiate the attenuated vaccine strain used in Japan from field isolates of Erysipelothrix rhusiopathiae found in pigs. However, this assay has been evaluated with only Japanese strains and isolates; therefore, it is unknown whether it could be used in other countries with E. rhusiopathiae strains and isolates of different genetic backgrounds. In our study, the PCR assay was evaluated using Chinese E. rhusiopathiae vaccine strains and field isolates. The PCR assay was able to differentiate the attenuated vaccine strains from the field isolates of E. rhusiopathiae in China but with a pattern different from that observed in Japan (only a single nucleotide polymorphism was detected in the Chinese vaccine strains compared with 5 in the Japanese vaccine strains). Importantly, either a DNA polymerase without 3' to 5' exonuclease activity or an exo+ polymerase with an antibody inhibiting the proofreading activity was required. In conclusion, after evaluation and improvement, this fast differentiation assay can be extended from Japan to China.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716665428
      Issue No: Vol. 28, No. 6 (2016)
       
  • Mycobacterium haemophilum infection in a juvenile leatherback sea turtle
           (Dermochelys coriacea)
    • Authors: Donnelly, K; Waltzek, T. B, Wellehan, J. F. X, Stacy, N. I, Chadam, M, Stacy, B. A.
      Pages: 718 - 721
      Abstract: Mycobacteriosis is infrequently reported in free-ranging sea turtles. Nontuberculous Mycobacterium haemophilum was identified as the causative agent of disseminated mycobacteriosis in a juvenile leatherback turtle (Dermochelys coriacea) that was found stranded on the Atlantic coast of Florida. Disseminated granulomatous inflammation was identified histologically, most notably affecting the nervous system. Identification of mycobacterial infection was based on cytologic, molecular, histologic, and microbiologic methods. Among stranded sea turtles received for diagnostic evaluation from the Atlantic and Gulf of Mexico coasts of the United States between 2004 and 2015, the diagnosis of mycobacteriosis was overrepresented in stranded oceanic-phase juveniles compared with larger size classes, which suggests potential differences in susceptibility or exposure among different life phases in this region. We describe M. haemophilum in a sea turtle, which contributes to the knowledge of diseases of small juvenile sea turtles, an especially cryptic life phase of the leatherback turtle.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716661746
      Issue No: Vol. 28, No. 6 (2016)
       
  • Canine mammary minute oncocytomas with neuroendocrine differentiation
           associated with multifocal acinar cell oncocytic metaplasia
    • Authors: Nagahara, R; Kimura, M, Itahashi, M, Sugahara, G, Kawashima, M, Murayama, H, Yoshida, T, Shibutani, M.
      Pages: 722 - 728
      Abstract: Two solitary and minute tumors of 1 and 1.5 mm diameter were identified by microscopy in the left fourth mammary gland of a 13-year-old female Labrador Retriever dog, in addition to multiple mammary gland tumors. The former tumors were well circumscribed and were composed of small-to-large polyhedral neoplastic oncocytes with finely granular eosinophilic cytoplasm, and were arranged in solid nests separated by fine fibrovascular septa. Scattered lumina of variable sizes containing eosinophilic secretory material were evident. Cellular atypia was minimal, and no mitotic figures were visible. One tumor had several oncocytic cellular foci revealing cellular transition, with perivascular pseudorosettes consisting of columnar epithelial cells surrounding the fine vasculature. Scattered foci of mammary acinar cell hyperplasia showing oncocytic metaplasia were also observed. Immunohistochemically, the cytoplasm of neoplastic cells of the 2 microtumors showed diffuse immunoreactivity to anti-cytokeratin antibody AE1/AE3, and finely granular immunoreactivity for 60-kDa heat shock protein, mitochondrial membrane ATP synthase complex V beta subunit, and chromogranin A. One tumor also had oncocytic cellular foci forming perivascular pseudorosettes showing cellular membrane immunoreactivity for neural cell adhesion molecule. The tumors were negative for smooth muscle actin, neuron-specific enolase, vimentin, desmin, S100, and synaptophysin. Ultrastructural observation confirmed the abundant mitochondria in the cytoplasm of both neoplastic and hyperplastic cells, the former cells also having neuroendocrine granule–like electron-dense bodies. From these results, our case was diagnosed with mammary oncocytomas accompanied by neuroendocrine differentiation. Scattered foci of mammary oncocytosis might be related to the multicentric occurrence of these oncocytomas.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716664381
      Issue No: Vol. 28, No. 6 (2016)
       
  • Coinfection by Cetacean morbillivirus and Aspergillus fumigatus in a
           juvenile bottlenose dolphin (Tursiops truncatus) in the Gulf of Mexico
    • Authors: Cassle, S. E; Landrau-Giovannetti, N, Farina, L. L, Leone, A, Wellehan, J. F. X, Stacy, N. I, Thompson, P, Herring, H, Mase-Guthrie, B, Blas-Machado, U, Saliki, J. T, Walsh, M. T, Waltzek, T. B.
      Pages: 729 - 734
      Abstract: A recently deceased juvenile male bottlenose dolphin (Tursiops truncatus) was found floating in the Gulf of Mexico, off Sand Key in Clearwater, Florida. At autopsy, we identified pneumonia and a focus of malacia in the right cerebrum. Cytologic evaluation of tissue imprints from the right cerebrum revealed fungal hyphae. Fungal cultures of the lung and brain yielded Aspergillus fumigatus, which was confirmed by amplification of a portion of the fungal nuclear ribosomal internal transcribed spacer 2 region sequence. Microscopic pulmonary lesions of bronchiolar epithelial cell syncytia with intracytoplasmic and intranuclear inclusions within bronchiolar epithelial cells were suggestive of Cetacean morbillivirus (CeMV) infection. The occurrence of CeMV infection was supported by positive immunohistochemical staining for morbillivirus antigen. CeMV detection was confirmed by amplification and sequencing a portion of the morbilliviral RNA-dependent RNA polymerase gene from lung tissue. This case provides CeMV sequence data available from the Gulf of Mexico and underscores the need for genomic sequencing across diverse host, temporospatial, and population (i.e., single animal vs. mass mortality events) scales to improve our understanding of these globally emerging pathogens.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716664761
      Issue No: Vol. 28, No. 6 (2016)
       
  • Elaeophora in the meninges of a Malayan sambar (Rusa unicolor equina)
    • Authors: Bernard, J; Grunenwald, C, Stalis, I. H, Varney, M, Zuba, J, Gerhold, R.
      Pages: 735 - 738
      Abstract: An adult nematode was grossly identified in the meninges of a Malayan sambar (Rusa unicolor equina), with numerous microfilariae associated with encephalitis and vasculitis on histopathology. The nematode was confirmed to be Elaeophora schneideri by sequencing a portion of the 18S rRNA gene. Our report highlights the potential for aberrant migration of E. schneideri in exotic deer species and the use of advanced testing to specifically identify this metazoan parasite, avoiding misidentification of Parelaphostrongylus tenuis.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716669388
      Issue No: Vol. 28, No. 6 (2016)
       
  • Hernia of the swim bladder (aerocystocele) with concurrent mycotic
           granulomatous inflammation and swim bladder carcinoma in a wild mullet
           (Mugil cephalus)
    • Authors: Sirri, R; Bianco, C, Zuccaro, G, Turba, M. E, Mandrioli, L.
      Pages: 739 - 743
      Abstract: We describe a hernia of the swim bladder, with a concurrent mycotic granulomatous inflammation, and carcinoma of the swim bladder in a wild mullet (Mugil cephalus) referred for an exophytic dorsal mass. Grossly, the mass was white, soft, and composed of multiple cystic gas-containing chambers connected by a funnel-shaped tissue segment to the coelomic swim bladder. Histologically, the mass was characterized by cysts of variable size, multifocally contiguous with the subepithelial rete mirabile, supported by abundant fibrous tissue. The skin covering the herniated swim bladder was focally ulcerated and replaced by abundant granulation tissue in which multiple scattered granulomas centered on pigmented fungal hyphae were observed. These granulomas were also seen in the remaining coelomic portion of the swim bladder as well as in the spleen, perivisceral pancreas, and peritoneal adipose tissue; the fungus was molecularly identified as Cladosporium spp. Focally, arising from the herniated swim bladder epithelium, an unencapsulated poorly demarcated, moderately cellular neoplasm, composed of islands, lobules, and acini of neoplastic epithelium, was found.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716663600
      Issue No: Vol. 28, No. 6 (2016)
       
  • Concurrent spindle-cell thymoma and thymic cysts in a Barbary sheep
           (Ammotragus lervia): case report and review of the literature
    • Authors: Li, W.-T; Chang, H.-W, Jeng, C.-R, Liu, C.-H, Wang, F.-I, Chang, L.-J, Pang, V. F.
      Pages: 744 - 749
      Abstract: An ~21-year-old female Barbary sheep (Ammotragus lervia) died spontaneously following a lengthy episode of difficulty in walking. An ~6 x 3 x 3 cm, unilocular cystic growth was found in the cranioventral thorax. The fibrotic cystic wall, lined by a single layer of flattened to cuboidal epithelial cells, was invaginated and partially encircled solid masses of fusiform neoplastic cells with multiple intratumoral cystic structures. The fusiform neoplastic cells were intensely positive for cytokeratin (CK) and partially positive for α–smooth muscle actin and vimentin, but negative for thyroid transcription factor–1 (TTF-1) and neuron-specific enolase (NSE). The intratumoral cysts were lined by CK-positive but TTF-1– negative, NSE-negative, flattened, cuboidal to columnar epithelial cells, suggestive of cystically dilated medullary duct epithelium-derived structures. Based on the location and histopathologic findings of the growth, concurrent spindle-cell thymoma and thymic cysts was diagnosed. We also discuss the correlation between thymic cysts and thymoma and review the literature of thymomas in ovine and wildlife species.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716665352
      Issue No: Vol. 28, No. 6 (2016)
       
  • Basal cell carcinoma in two Hermanns tortoises (Testudo hermanni)
    • Authors: Hellebuyck, T; Ducatelle, R, Bosseler, L, Van Caelenberg, A, Versnaeyen, H, Chiers, K, Martel, A.
      Pages: 750 - 754
      Abstract: Neoplastic disorders are frequently encountered in the practice of reptile medicine. Herein we report the clinical behavior, antemortem diagnosis, and histopathologic characteristics of a recurrent intraoral keratinizing basal cell carcinoma (BCC) and a metastatic BCC of the carapace in 2 Hermann’s tortoises (Testudo hermanni). Although squamous cell carcinomas (SCCs) in tortoises show similar predilection sites and gross pathologic features, the BCCs described in our report were characterized by a remarkably fast and highly infiltrative growth in comparison to SCCs. Accordingly, early diagnosis including reliable discrimination from SCC is essential toward the management of this neoplastic entity in tortoises.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716668560
      Issue No: Vol. 28, No. 6 (2016)
       
  • Cross-reactions in specific Brachyspira spp. PCR assays caused by
           "Brachyspira hampsonii" isolates: implications for detection
    • Authors: Aller-Moran, L. M; Martinez-Lobo, F. J, Rubio, P, Carvajal, A.
      Pages: 755 - 759
      Abstract: An emerging novel spirochete in swine, provisionally designated "Brachyspira hampsonii," has been detected worldwide. It has been associated with swine dysentery and cannot be differentiated from B. hyodysenteriae, the classical etiologic agent of this disease, using standard phenotypic methods. We evaluated cross-reactions of "B. hampsonii" isolates recovered from avian species in some of the currently available species-specific polymerase chain reaction (PCR) assays for the identification of swine Brachyspira species. Ten avian "B. hampsonii" isolates recovered from wild waterfowl were used. No false-positive results were recorded with a B. pilosicoli–specific PCR based on the amplification of a fragment of the 16S rRNA gene. However, the percentage of false-positive results varied, with a range of 10–80%, in the evaluated B. hyodysenteriae–specific assays based on the amplification of the 23S rRNA, nox, and tlyA genes. Similarly, results of the B. intermedia–specific PCR assays yielded poor specificity, with up to 80% of the "B. hampsonii" isolates tested giving false-positive results. Finally, 2 "B. hampsonii" avian isolates yielded a positive result in a B. innocens– and B. murdochii–specific PCR. This result should be interpreted very cautiously as these 2 isolates could represent a recombinant genotype.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716667528
      Issue No: Vol. 28, No. 6 (2016)
       
  • Pseudomonas aeruginosa mastitis in two goats associated with an essential
           oil-based teat dip
    • Authors: Kelly, E. J; Wilson, D. J.
      Pages: 760 - 762
      Abstract: Pseudomonas aeruginosa is an opportunistic pathogen that has been associated with mastitis in dairy animals, including goats. Often, the environmental sources of the bacteria are water-related (such as hoses and muddy pastures). Mastitis attributable to P. aeruginosa was identified in 2 goats in a small herd. Efforts were made to identify environmental sources of the pathogen. Multiple samples from the goats’ environment were cultured, including water from the trough, bedding, the hose used to wash udders, and the teat dip and teat dip containers. The bacterium was isolated from the teat dip and the teat dip container. The teat dip consisted of water, liquid soap, and several drops of essential oils (including tea tree, lavender, and peppermint). This case illustrates a potential problem that may arise as a result of the use of unconventional ingredients in teat dips. The use of alternative products by goat producers is likely to increase in the future.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716672255
      Issue No: Vol. 28, No. 6 (2016)
       
  • Isolation and identification of Caviibacter abscessus from cervical
           abscesses in a series of pet guinea pigs (Cavia porcellus)
    • Authors: Bemis, D. A; Johnson, B. H, Bryant, M. J, Jones, R. D, McCleery, B. V, Greenacre, C. B, Perreten, V, Kania, S. A.
      Pages: 763 - 769
      Abstract: An organism reported in the early literature to be a rare cause of cervical lymphadenitis in guinea pigs, Streptobacillus moniliformis, has been reclassified as Caviibacter abscessus. We describe a series of sequential cases of abscesses in guinea pigs that were presented to our clinic from which the only agent isolated was a unique, serum-requiring bacterium. Discrete colonies were not detected in 6.5% CO2 or anaerobically on routine primary isolation media containing up to 5% whole sheep blood, with and without cysteine, vitamin K, and hemin supplementation after 7 days of incubation at 37°C. Based on subsequently determined growth requirements, the organisms were best described as serum-requiring, aerotolerant anaerobes. Colonies were detectable within 24 h at 37°C in an anaerobic atmosphere on a mycoplasma agar–based medium containing 10% pig serum and reached 3 mm in diameter within 3–5 days. Microscopic appearance consisted of small gram-negative rods and coccobacilli with occasional filaments. However, in direct smears from clinical specimens and from weak or dysgonic growth on plates incubated under suboptimal growth conditions (e.g., in 6.5% CO2), irregular rods with occasional small bulbous forms or numerous long wavy filaments were observed. All of the isolates generated unique spectral profiles similar to that of C. abscessus when examined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Phylogenetic analysis of partial 16S rRNA gene sequences showed that the isolates were identical to each other and shared 99.9% sequence identity with C. abscessus.
      PubDate: 2016-10-13T12:55:12-07:00
      DOI: 10.1177/1040638716665660
      Issue No: Vol. 28, No. 6 (2016)
       
 
 
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