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  Subjects -> VETERINARY SCIENCE (Total: 210 journals)
Acta Scientiae Veterinariae     Open Access  
Acta Veterinaria     Open Access   (Followers: 1)
Acta Veterinaria Brasilica     Open Access  
Acta Veterinaria Hungarica     Full-text available via subscription   (Followers: 1)
Acta Veterinaria Scandinavica     Open Access   (Followers: 1)
Advances in Animal Biosciences     Full-text available via subscription   (Followers: 7)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 9)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 14)
African Journal of Wildlife Research     Full-text available via subscription  
Alexandria Journal of Veterinary Sciences     Open Access  
American Journal of Animal and Veterinary Sciences     Open Access   (Followers: 10)
American Journal of Primatology     Hybrid Journal   (Followers: 7)
American Journal of Veterinary Research     Full-text available via subscription   (Followers: 21)
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C     Hybrid Journal   (Followers: 2)
Animal Behaviour     Hybrid Journal   (Followers: 194)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5)
Animal Health Research Reviews     Hybrid Journal   (Followers: 4)
Animal Nutrition     Open Access   (Followers: 5)
Animal Reproduction     Open Access  
Animal Reproduction Science     Hybrid Journal   (Followers: 6)
Animals     Open Access   (Followers: 6)
Annals of Agricultural and Environmental Medicine     Open Access   (Followers: 1)
Annual Review of Animal Biosciences     Full-text available via subscription   (Followers: 5)
Anthrozoos : A Multidisciplinary Journal of The Interactions of People & Animals     Hybrid Journal   (Followers: 8)
Archives of Animal Nutrition     Hybrid Journal   (Followers: 7)
Archivos de Medicina Veterinaria     Open Access   (Followers: 1)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access   (Followers: 1)
Arquivos de Ciências Veterinárias e Zoologia da UNIPAR     Open Access  
Ars Veterinaria     Open Access  
Asian Journal of Medical and Biological Research     Open Access  
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atatürk Üniversitesi Veteriner Bilimleri Dergisi     Open Access  
Australian Equine Veterinarian     Full-text available via subscription   (Followers: 2)
Australian Veterinary Journal     Hybrid Journal   (Followers: 12)
Avances en Ciencias Veterinarias     Open Access  
Avian Diseases     Full-text available via subscription   (Followers: 5)
Avian Diseases Digest     Full-text available via subscription   (Followers: 3)
Avian Pathology     Hybrid Journal   (Followers: 3)
Bangladesh Journal of Animal Science     Open Access  
Bangladesh Journal of Veterinary Medicine     Open Access   (Followers: 1)
Bangladesh Veterinarian     Open Access   (Followers: 1)
BMC Veterinary Research     Open Access   (Followers: 9)
Brazilian Journal of Veterinary Research and Animal Science     Open Access   (Followers: 7)
Buletin Peternakan : Bulletin of Animal Science     Full-text available via subscription  
Bulletin of Animal Health and Production in Africa     Full-text available via subscription   (Followers: 1)
Bulletin of the Veterinary Institute in Pulawy     Open Access  
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access  
Canadian Journal of Veterinary Research     Full-text available via subscription   (Followers: 8)
Case Reports in Veterinary Medicine     Open Access   (Followers: 4)
Ciência Animal Brasileira     Open Access  
Ciência Rural     Open Access   (Followers: 2)
Cogent Food & Agriculture     Open Access  
Companion Animal     Full-text available via subscription   (Followers: 5)
Domestic Animal Endocrinology     Hybrid Journal   (Followers: 2)
Equine Health     Full-text available via subscription   (Followers: 1)
Equine Veterinary Education     Hybrid Journal   (Followers: 8)
Equine Veterinary Journal     Hybrid Journal   (Followers: 11)
Ethiopian Veterinary Journal     Open Access   (Followers: 4)
Eurasian Journal of Veterinary Sciences     Open Access   (Followers: 1)
Frontiers in Veterinary Science     Open Access  
Global Journal of Animal Scientific Research     Open Access   (Followers: 1)
Human & Veterinary Medicine - International Journal of the Bioflux Society     Open Access   (Followers: 6)
ILAR Journal     Hybrid Journal  
In Practice     Full-text available via subscription   (Followers: 3)
Indian Journal of Animal Sciences     Open Access   (Followers: 5)
Indian Journal of Veterinary Anatomy     Full-text available via subscription   (Followers: 2)
Intas Polivet     Full-text available via subscription  
International Journal for Agro Veterinary and Medical Sciences     Open Access   (Followers: 4)
International Journal of Livestock Research     Open Access  
International Journal of Veterinary Science and Medicine     Open Access   (Followers: 6)
InVet     Open Access  
Iranian Journal of Applied Animal Science     Open Access  
Irish Veterinary Journal     Open Access   (Followers: 4)
İstanbul Üniversitesi Veteriner Fakültesi Dergisi     Open Access  
Journal of Veterinary Science & Technology     Open Access   (Followers: 4)
Journal of Advanced Veterinary and Animal Research     Open Access   (Followers: 8)
Journal of Animal Behaviour and Biometeorology     Open Access   (Followers: 2)
Journal of Animal Physiology and Animal Nutrition     Hybrid Journal   (Followers: 5)
Journal of Animal Science and Technology     Open Access  
Journal of Applied Animal Nutrition     Hybrid Journal   (Followers: 3)
Journal of Avian Medicine and Surgery     Full-text available via subscription   (Followers: 5)
Journal of Buffalo Science     Hybrid Journal  
Journal of Equine Veterinary Science     Hybrid Journal   (Followers: 10)
Journal of Exotic Pet Medicine     Full-text available via subscription   (Followers: 4)
Journal of Experimental and Applied Animal Sciences     Open Access  
Journal of Feline Medicine & Surgery     Hybrid Journal   (Followers: 5)
Journal of Research in Forestry, Wildlife and Environment     Open Access   (Followers: 3)
Journal of Small Animal Practice     Hybrid Journal   (Followers: 12)
Journal of the American Veterinary Medical Association     Full-text available via subscription   (Followers: 27)
Journal of the Selva Andina Research Society     Open Access  
Journal of the South African Veterinary Association     Open Access   (Followers: 1)
Journal of Venomous Animals and Toxins     Open Access   (Followers: 4)
Journal of Veterinary Advances     Open Access   (Followers: 3)
Journal of Veterinary Behavior: Clinical Applications and Research     Hybrid Journal   (Followers: 3)
Journal of Veterinary Cardiology     Full-text available via subscription   (Followers: 6)
Journal of Veterinary Diagnostic Investigation     Hybrid Journal   (Followers: 8)
Journal of Veterinary Emergency and Critical Care     Hybrid Journal   (Followers: 16)
Journal of Veterinary Internal Medicine     Open Access   (Followers: 17)
Journal of Veterinary Medical Education     Partially Free   (Followers: 12)
Journal of Veterinary Medicine     Open Access   (Followers: 6)

        1 2 3     

Journal Cover   Journal of Veterinary Diagnostic Investigation
  [SJR: 0.677]   [H-I: 53]   [8 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1040-6387 - ISSN (Online) 1943-4936
   Published by Sage Publications Homepage  [827 journals]
  • Letter to the Editor
    • Authors: Saliki, J; Jensen, W. A.
      Pages: 664 - 664
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715615114
      Issue No: Vol. 27, No. 6 (2015)
  • Technological advances in bovine mastitis diagnosis: an overview
    • Authors: Duarte, C. M; Freitas, P. P, Bexiga, R.
      Pages: 665 - 672
      Abstract: Bovine mastitis is an economic burden for dairy farmers and preventive control measures are crucial for the sustainability of any dairy business. The identification of etiological agents is necessary in controlling the disease, reducing risk of chronic infections and targeting antimicrobial therapy. The suitability of a detection method for routine diagnosis depends on several factors, including specificity, sensitivity, cost, time in producing results, and suitability for large-scale sampling of milk. This article focuses on current methodologies for identification of mastitis pathogens and for detection of inflammation, as well as the advantages and disadvantages of different methods. Emerging technologies, such as transcriptome and proteome analyses and nano- and microfabrication of portable devices, offer promising, sensitive methods for advanced detection of mastitis pathogens and biomarkers of inflammation. The demand for alternative, fast, and reliable diagnostic procedures is rising as farms become bigger. Several examples of technological and scientific advances are summarized which have given rise to more sensitive, reliable and faster diagnostic results.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715603087
      Issue No: Vol. 27, No. 6 (2015)
  • A review of traditional and contemporary assays for direct and indirect
           detection of Equid herpesvirus 1 in clinical samples
    • Authors: Balasuriya, U. B. R; Crossley, B. M, Timoney, P. J.
      Pages: 673 - 687
      Abstract: Equid herpesvirus 1 (EHV-1) is one of the most economically important equine viral pathogens. Its clinical manifestations in horses vary from acute upper respiratory tract disease, abortion, or neonatal death, to neurological disease termed equine herpesviral myeloencephalopathy, which may lead to paralysis and a fatal outcome. Successful identification of EHV-1 infection in horses depends on a variety of factors such as suitable case selection with emphasis on timing of sample collection, selection of appropriate sample(s) based on the clinical manifestations, application of relevant diagnostic technique(s) and/or test(s), and careful evaluation and interpretation of laboratory results. Several traditional serologic and virus isolation assays have been described; however, these assays have inherent limitations that prevent rapid and reliable detection of EHV-1. The advent of molecular biologic techniques has revolutionized the diagnosis of infectious diseases in humans and animal species. Specifically, polymerase chain reaction (PCR)–based assays have allowed detection of nucleic acid in clinical specimens precisely and rapidly as compared to the traditional methods that detect the agent or antigen, or agent-specific antibodies in serum. The new molecular methods, especially real-time PCR, can be a very useful means of EHV-1 detection and identification. Veterinarians involved in equine practice must be aware of the advantages and disadvantages of various real-time PCR assays, interpretation of viral genetic marker(s), and latency in order to provide the best standard of care for their equine patients.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715605558
      Issue No: Vol. 27, No. 6 (2015)
  • Protein and cholesterol electrophoresis of plasma samples from captive
           cownose ray (Rhinoptera bonasus)
    • Authors: Cray, C; Rodriguez, M, Field, C, McDermott, A, Leppert, L, Clauss, T, Bossart, G. D.
      Pages: 688 - 695
      Abstract: Our study was undertaken to assess the application of semiautomated methods available at the reference laboratory level for the evaluation of plasma protein and cholesterol via electrophoresis in samples from cownose rays (Rhinoptera bonasus). Three groups of animals were assessed: clinically normal, clinically abnormal, and parasitized with leeches. As reported previously, the albumin band was negligible; the protein electrophoretograms were dominated by a large beta-globulin fraction. While the group of samples from the leech-parasitized rays did not show any large differences, the abnormal group exhibited significantly elevated total solids and cholesterol levels. The latter was related to a significant increase in very low density lipoprotein levels. The results demonstrate the potential application of these laboratory methods in quantitation of plasma proteins and cholesterol fractions in subclass Elasmobranchii.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715607293
      Issue No: Vol. 27, No. 6 (2015)
  • A comparison of two real-time polymerase chain reaction assays using
           hybridization probes targeting either 16S ribosomal RNA or a subsurface
           lipoprotein gene for detecting leptospires in canine urine
    • Authors: Gentilini, F; Zanoni, R. G, Zambon, E, Turba, M. E.
      Pages: 696 - 703
      Abstract: Leptospires are excreted in the urine of infected animals, and the prompt detection of leptospiral DNA using polymerase chain reaction (PCR) is increasingly being used. However, contradictory data has emerged concerning the diagnostic accuracy of the most popular PCR assays that target either the 16S ribosomal RNA (rrs) or the subsurface lipoprotein (LipL32) genes. In order to clarify the effect of the gene target, a novel hydrolysis probe–based, quantitative real-time PCR (qPCR) assay targeting the LipL32 gene was developed, validated, and then compared directly to the previously described rrs hydrolysis probe–based qPCR using a convenience collection of canine urine samples. The novel LipL32 qPCR assay was linear from 5.9 x 106 to 59 genome equivalents per reaction. Both the LipL32 and the rrs qPCR assays showed a limit of detection of 10 target copies per reaction indicating an approximately equivalent analytical sensitivity. Both assays amplified all 20 pathogenic leptospiral strains tested but did not amplify a representative collection of bacteria commonly found in voided canine urine. When the field samples were assayed, 1 and 5 out of 184 samples yielded an amplification signal in the LipL32 and rrs assays, respectively. Nevertheless, when the limit of detection was considered as the cutoff for interpreting findings, the 4 discordant cases were judged as negative. In conclusion, our study confirmed that both LipL32 and rrs are suitable targets for qPCR for the detection of leptospiral DNA in canine urine. However, the rrs target requires the mandatory use of a cutoff value in order to correctly interpret spurious amplifications.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715610378
      Issue No: Vol. 27, No. 6 (2015)
  • A comparative evaluation of feathers, oropharyngeal swabs, and cloacal
           swabs for the detection of H5N1 highly pathogenic avian influenza virus
           infection in experimentally infected chickens and ducks
    • Authors: Nuradji, H; Bingham, J, Lowther, S, Wibawa, H, Colling, A, Long, N. T, Meers, J.
      Pages: 704 - 715
      Abstract: Oropharyngeal and cloacal swabs have been widely used for the detection of H5N1 highly pathogenic avian Influenza A virus (HPAI virus) in birds. Previous studies have shown that the feather calamus is a site of H5N1 virus replication and therefore has potential for diagnosis of avian influenza. However, studies characterizing the value of feathers for this purpose are not available, to our knowledge; herein we present a study investigating feathers for detection of H5N1 virus. Ducks and chickens were experimentally infected with H5N1 HPAI virus belonging to 1 of 3 clades (Indonesian clades 2.1.1 and 2.1.3, Vietnamese clade 1). Different types of feathers and oropharyngeal and cloacal swab samples were compared by virus isolation. In chickens, virus was detected from all sample types: oral and cloacal swabs, and immature pectorosternal, flight, and tail feathers. During clinical disease, the viral titers were higher in feathers than swabs. In ducks, the proportion of virus-positive samples was variable depending on viral strain and time from challenge; cloacal swabs and mature pectorosternal feathers were clearly inferior to oral swabs and immature pectorosternal, tail, and flight feathers. In ducks infected with Indonesian strains, in which most birds did not develop clinical signs, all sampling methods gave intermittent positive results; 3–23% of immature pectorosternal feathers were positive during the acute infection period; oropharyngeal swabs had slightly higher positivity during early infection, while feathers performed better during late infection. Our results indicate that immature feathers are an alternative sample for the diagnosis of HPAI in chickens and ducks.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715611443
      Issue No: Vol. 27, No. 6 (2015)
  • Breed-specific reference intervals for assessing thyroid function in seven
           dog breeds
    • Authors: Hegstad-Davies, R. L; Torres, S. M. F, Sharkey, L. C, Gresch, S. C, Munoz-Zanzi, C. A, Davies, P. R.
      Pages: 716 - 727
      Abstract: Thyroxine (T4), free T4 (FT4), and thyrotropin (TSH) concentrations were measured in serum from 693 healthy representatives from 7 dog breeds (Alaskan Malamute, Collie, English Setter, Golden Retriever, Keeshond, Samoyed, or Siberian Husky) to determine whether breed-specific reference intervals (RIs) are warranted. Veterinarians reviewed the health history, performed a physical examination, and approved laboratory data for the enrolled dogs. Many purebred dogs had T4 and FT4 concentrations that were at, or below, the lower limits previously determined for non–breed-specific RIs. Mean concentrations of T4, FT4, and TSH varied significantly among breeds. The range of mean concentration of T4 (19.7 nmol/L [1.53 µg/dL] in English Setters to 29.0 nmol/L [2.25 µg/dL] in Keeshonds) and FT4 (12.6 pmol/L [0.98 ng/dL] in English Setters to 20.2 pmol/L [1.57 ng/dL] in Samoyeds) was considerable. Median TSH values ranged from 6.10 mIU/L (0.07 ng/mL; Alaskan Malamute and Golden Retriever) to 17.6 mIU/L (0.26 ng/mL; Collie). Mean T4 and FT4 concentrations were higher in females. Increasing age was associated with decreasing T4 and FT4, and increasing TSH concentration. The substantial ranges across breeds of measures of central tendency (mean, median) for all hormones indicate that breed-specific RIs are warranted. RIs encompassing the central 95% of reference values for all breeds combined, and for individual breeds, were calculated using nonparametric (TSH) and robust (T4, FT4) methods. Use of breed-specific RIs in combination with careful attention to the potential for pre-analytical and analytical variability in test results will improve thyroid function assessment in these breeds.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715606953
      Issue No: Vol. 27, No. 6 (2015)
  • Enhanced sensitivity of an antibody competitive blocking enzyme-linked
           immunosorbent assay using Equine arteritis virus purified by
           anion-exchange membrane chromatography
    • Authors: Chung, C. J; Grimm, A. L, Wilson, C. L, Balasuriya, U. B. R, Chung, G, Timoney, P. J, Bandaranayaka-Mudiyanselage, C.-B, Lee, S. S, McGuire, T. C.
      Pages: 728 - 738
      Abstract: In an effort to improve a competitive blocking enzyme-linked immunosorbent assay (cELISA) for antibody detection to Equine arteritis virus (EAV), antigen purified by anion-exchange membrane chromatography capsule (AEC) was evaluated. Virus purification by the AEC method was rapid and easily scalable. A comparison was made between virus purified by the AEC method with that obtained by differential centrifugation based on the following: 1) the relative purity and quality of EAV glycoprotein 5 (GP5) containing the epitope defined by monoclonal antibody 17B7, and 2) the relative sensitivity of a commercial antibody cELISA with the only change being the 2 purified antigens. On evaluation by Western blot using GP5-specific monoclonal antibody 17B7, the AEC-purified EAV contained 86% GP5 monomer whereas the differentially centrifuged EAV contained
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715606487
      Issue No: Vol. 27, No. 6 (2015)
  • Development of an immunochromatographic strip for antibody detection of
           pseudorabies virus in swine
    • Authors: Li, X; Sun, Y, Yang, S, Wang, Y, Yang, J, Liu, Y, Jin, Q, Li, X, Guo, C, Zhang, G.
      Pages: 739 - 742
      Abstract: An immunochromatographic strip was developed for the serological detection of pseudorabies virus (PRV) in swine. In the strip, the expressed protein of gB, one of the glycoproteins of PRV, labeled with colloidal gold, was used as the detector; staphylococcal protein A and swine anti–pseudorabies virus antibody were blotted on nitrocellulose membrane for the test and control lines, respectively. The specificity of the strip was 98.1%, and the sensitivity of the strip with reference anti-PRV serum was 96.0%. Swine serum samples (296) were collected to evaluate the characteristics of the strip in comparison with an existing commercial kit. The agreement was 93.6%. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents, or equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715611442
      Issue No: Vol. 27, No. 6 (2015)
  • A retrospective study of skull base neoplasia in 42 dogs
    • Authors: Rissi; D. R.
      Pages: 743 - 748
      Abstract: This study describes the prevalence and distribution of 42 cases of skull base neoplasia in dogs between 2000 and 2014. The average age of affected individuals was 9.5 years, and there was no sex or breed predisposition. The most common skull base neoplasms were meningioma (25 cases) and pituitary adenoma (9 cases). Less common tumors included craniopharyngioma (2 cases), nerve sheath tumor (2 cases), and 1 case each of pituitary carcinoma, meningeal oligodendrogliomatosis, presumed nasal or sinonasal carcinoma, and multilobular tumor of bone. All neoplasms caused some degree of compression of adjacent structures. The distribution of the tumors was greatest in the sellar region (n = 18), followed by the paranasal region (n = 12), caudal cranial fossa (n = 10), central cranial fossa (n = 1), and rostral cranial fossa (n = 1).
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715611706
      Issue No: Vol. 27, No. 6 (2015)
  • Abortion in a Mediterranean miniature donkey (Equus asinus) associated
           with a gammaherpesvirus similar to Equid herpesvirus 7
    • Authors: LeCuyer, T. E; Rink, A, Bradway, D. S, Evermann, J. F, Nicola, A. V, Baszler, T, Haldorson, G. J.
      Pages: 749 - 753
      Abstract: Fetal tissues and placenta from a third trimester Mediterranean miniature donkey (Equus asinus) abortion were submitted to the Washington State University, Washington Animal Disease Diagnostic Laboratory for abortion diagnosis. Microscopic examination of formalin-fixed tissues revealed multifocal necrotizing placentitis. Several cells within the necrotic foci contained large, eosinophilic, intranuclear inclusions. Virus isolation from fresh, frozen placenta identified a cytopathic, syncytia-forming virus. Polymerase chain reaction (PCR) from the cultured virus using degenerate universal herpesvirus primers amplified a 699–base pair portion of the DNA polymerase gene. The PCR amplicon had 96.7% nucleotide identity with the DNA polymerase gene of Equid herpesvirus 7 (EHV-7; asinine herpesvirus 2), a gammaherpesvirus. An identical sequence was obtained when the same degenerate herpesvirus primers were used for PCR on the formalin-fixed placenta. Additionally, the amplicon had complete identity with short sequences of asinine herpesviruses that have been published in association with interstitial pneumonia in donkeys. EHV-7 has previously been isolated from nasal secretions of normal donkeys and mules. Our report describes a case of abortion associated with EHV-7 or a similar virus.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715611444
      Issue No: Vol. 27, No. 6 (2015)
  • The use of loop-mediated isothermal amplification improves Toxoplasma
           gondii detection in wildlife
    • Authors: Trisciuoglio, A; Zanet, S, Marello, G, Chiesa, F, Nucera, D. M, Bergallo, M, Gennero, M. S, Ferroglio, E.
      Pages: 754 - 757
      Abstract: Toxoplasma gondii is among the most widespread parasites worldwide. Wildlife is recognized as an important reservoir and source of infection of T. gondii. The goal of the present work was to assess the performance of loop-mediated isothermal amplification (LAMP) as a diagnostic tool for T. gondii infection in the skeletal muscle and central nervous system (CNS) of free-ranging ungulates and carnivores. Fifty-seven wild animals were tested for the presence of T. gondii DNA by polymerase chain reaction (PCR) and LAMP. The use of LAMP amplification improved sensitivity in T. gondii molecular detection compared with conventional PCR on skeletal muscle (2 = 5.8, P < 0.05), having a lower minimum detection limit (0.1 tachyzoite) than PCR (1 tachyzoite). No significant differences existed between the detection capacities of both assays when performed on CNS. LAMP is a valid tool to improve the diagnosis of T. gondii infection in wild game meat. The technique provides a sensitive yet specific method that can be applicable to both field surveys and large-scale testing of wildlife samples.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715611170
      Issue No: Vol. 27, No. 6 (2015)
  • Canid herpesvirus 1 (CHV-1)-related disease in older puppies and CHV-1
           shedding in the vagina of adult pregnant dogs
    • Authors: Kapil; S.
      Pages: 758 - 761
      Abstract: A large breeding kennel of Bulldogs (n = 57) experienced several Canid herpesvirus 1 (CHV-1)–related diseases in older puppies (9 weeks of age) in Arkansas. CHV-1 has been repeatedly confirmed in the kennel in several animals for 3 years (January 2012–February 2015) using various virology tests. I was able to detect a partial sequence of CHV DNA (~120 bp) in archived formalin-fixed, paraffin-embedded tissue blocks after 3 years of storage. CHV-1 is persistently circulating in this kennel in spite of high serum antibody titers in the adult dogs. The dogs were negative for canine brucellosis antibodies based on Brucella canis rapid card test.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715610377
      Issue No: Vol. 27, No. 6 (2015)
  • Chronic inflammatory demyelinating polyradiculoneuropathy with cholesterol
           deposits in a dog
    • Authors: Pineyro, P; Sponenberg, D. P, Pancotto, T, King, R. H. M, Jortner, B. S.
      Pages: 762 - 766
      Abstract: Chronic inflammatory demyelinating polyradiculoneuropathy occurred in an 11-year-old Labrador Retriever dog. Spinal cord compression resulted from massive radiculitis with prominent cholesterol granulomas. Cholesterol deposition and associated granuloma formation is unique in chronic inflammatory demyelinating polyradiculoneuropathy, in both its human and canine expressions.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715610379
      Issue No: Vol. 27, No. 6 (2015)
  • Disseminated mycobacteriosis manifesting as paraplegia in two Parma
           wallabies (Macropus parma) naturally exposed to Mycobacterium avium
    • Authors: Robveille, C; Albaric, O, Gaide, N, Abadie, J.
      Pages: 767 - 771
      Abstract: Two captive female Parma wallabies (Macropus parma) died after a history of flaccid paraplegia. On postmortem examination, granulomatous and suppurative osteomyelitis involving the left ischium and the lumbosacral region, with meningeal extension at the cauda equina, and caseonecrotic mastitis were the most significant changes. Multiple small nodules in the liver and spleen, and an enlargement of some lymph nodes with central caseous necrosis were also observed. Microscopically, a disseminated granulomatous inflammation with numerous multinucleate giant cells was seen. Numerous acid-fast bacilli were detected in macrophages, in multinucleated giant cells, and free in the central necrosis and suppurative exudate. After culture, polymerase chain reaction assays were carried out to detect the 65-kDa heat shock protein (Hsp65) and insertion sequences (IS)1245 and IS900. The causative agent was identified as Mycobacterium avium subsp. avium.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715608897
      Issue No: Vol. 27, No. 6 (2015)
  • An unusual lipomatous brain mass in a Golden Retriever dog
    • Authors: Scott, S. J; Elliot, K, Philibert, H, Summers, B. A, Godson, D, Singh, B, Simko, E.
      Pages: 772 - 776
      Abstract: A 9-year-old Golden Retriever dog was presented to the Veterinary Medical Center with a 3-week history of grand mal seizures and was subsequently euthanized. At autopsy, a discrete, firm, expansile mass was found in the right pyriform lobe, which compressed the ipsilateral hippocampus, thalamus, and cerebral cortex. Histologically, the mass was composed of well-differentiated adipose tissue supported by fibrous and mucinous stroma. Adipocytes exhibited strong immunoreactivity for vimentin and were negative for pancytokeratin (AE1/AE3), glial fibrillary acidic protein, neuron-specific enolase, and synaptophysin. These findings are most compatible with an intracranial lipomatous hamartoma, which is an extraparenchymal lesion that has been identified in several species. The current report describes an intracerebral lipomatous hamartoma in a veterinary species.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715608216
      Issue No: Vol. 27, No. 6 (2015)
  • Severe Mycoplasma bovis outbreak in an Austrian dairy herd
    • Pages: 777 - 783
      Abstract: A conventional dairy farm, housing 19 Austrian Simmental cows, experienced a spontaneous outbreak of a Mycoplasma bovis infection, showing severe clinical signs of respiratory tract disease, clinical mastitis, and tremendous drop in milk production. Despite intensive therapy, 5 cows died within 2 weeks or were euthanized. From the remaining cows, bacteriological culture and polymerase chain reaction revealed M. bovis in 10 of 14 milk samples. Mycoplasma bovis was found in 1 of 5 randomly collected nasal swabs. Autopsy of 1 cow revealed infection of the lungs and the udder with M. bovis. The 13 M. bovis isolates from milk samples, nasal swabs, lungs, and udder were genotyped by multilocus variable number of tandem-repeat analysis, and indicated that described infections were caused by a single M. bovis strain. The virulent M. bovis strain resulted in dramatic economic loss to the farmer. To control the disease, culling of all animals, including heifers and calves, was recommended, and strict hygienic measures were implemented before introducing new animals to the farm.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715603088
      Issue No: Vol. 27, No. 6 (2015)
  • Sensitive detection of Porcine circovirus-2 by droplet digital polymerase
           chain reaction
    • Authors: Zhao, S; Lin, H, Chen, S, Yang, M, Yan, Q, Wen, C, Hao, Z, Yan, Y, Sun, Y, Hu, J, Chen, Z, Xi, L.
      Pages: 784 - 788
      Abstract: Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 25 copies/μL, a 4-fold greater sensitivity than TaqMan real-time PCR. Both methods showed a high degree of linearity (R 2 = ~1), although TaqMan real-time PCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might represent a promising platform for detecting PCV-2 viral loads.
      PubDate: 2015-11-24T10:26:08-08:00
      DOI: 10.1177/1040638715608358
      Issue No: Vol. 27, No. 6 (2015)
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