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  Subjects -> VETERINARY SCIENCE (Total: 215 journals)
Showing 1 - 63 of 63 Journals sorted alphabetically
Acta Scientiae Veterinariae     Open Access   (Followers: 1)
Acta Veterinaria     Open Access   (Followers: 1)
Acta Veterinaria Brasilica     Open Access  
Acta Veterinaria Hungarica     Full-text available via subscription   (Followers: 2)
Acta Veterinaria Scandinavica     Open Access   (Followers: 1)
Advances in Animal Biosciences     Full-text available via subscription   (Followers: 9)
Advances in Small Animal Medicine and Surgery     Hybrid Journal  
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 12)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 12)
African Journal of Wildlife Research     Full-text available via subscription   (Followers: 1)
Alexandria Journal of Veterinary Sciences     Open Access  
American Journal of Animal and Veterinary Sciences     Open Access   (Followers: 8)
American Journal of Primatology     Hybrid Journal   (Followers: 11)
American Journal of Veterinary Research     Full-text available via subscription   (Followers: 21)
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C     Hybrid Journal   (Followers: 3)
Animal Behaviour     Hybrid Journal   (Followers: 106)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5)
Animal Health Research Reviews     Hybrid Journal   (Followers: 3)
Animal Nutrition     Open Access   (Followers: 7)
Animal Reproduction     Open Access   (Followers: 1)
Animal Reproduction Science     Hybrid Journal   (Followers: 4)
Animals     Open Access   (Followers: 7)
Annals of Agricultural and Environmental Medicine     Open Access   (Followers: 1)
Annual Review of Animal Biosciences     Full-text available via subscription   (Followers: 4)
Anthrozoos : A Multidisciplinary Journal of The Interactions of People & Animals     Hybrid Journal   (Followers: 6)
Archives of Animal Nutrition     Hybrid Journal   (Followers: 6)
Archivos de Medicina Veterinaria     Open Access   (Followers: 1)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access  
Arquivos de Ciências Veterinárias e Zoologia da UNIPAR     Open Access  
Ars Veterinaria     Open Access  
Asian Journal of Medical and Biological Research     Open Access   (Followers: 1)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atatürk Üniversitesi Veteriner Bilimleri Dergisi     Open Access  
Australian Equine Veterinarian     Full-text available via subscription   (Followers: 3)
Australian Veterinary Journal     Hybrid Journal   (Followers: 15)
Avances en Ciencias Veterinarias     Open Access  
Avian Diseases     Full-text available via subscription   (Followers: 4)
Avian Pathology     Hybrid Journal   (Followers: 2)
Bangladesh Journal of Animal Science     Open Access  
Bangladesh Journal of Veterinary Medicine     Open Access   (Followers: 1)
Bangladesh Veterinarian     Open Access  
BMC Veterinary Research     Open Access   (Followers: 10)
Brazilian Journal of Veterinary Research and Animal Science     Open Access   (Followers: 8)
Buletin Peternakan : Bulletin of Animal Science     Full-text available via subscription  
Buletin Veteriner Udayana     Open Access  
Bulletin of Animal Health and Production in Africa     Full-text available via subscription   (Followers: 1)
Bulletin of the Veterinary Institute in Pulawy     Open Access  
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access  
Canadian Journal of Veterinary Research     Full-text available via subscription   (Followers: 8)
Case Reports in Veterinary Medicine     Open Access   (Followers: 5)
Ciência Animal Brasileira     Open Access  
Ciência Rural     Open Access   (Followers: 1)
Cogent Food & Agriculture     Open Access  
Companion Animal     Full-text available via subscription   (Followers: 9)
Domestic Animal Endocrinology     Hybrid Journal   (Followers: 4)
Equine Health     Full-text available via subscription   (Followers: 3)
Equine Veterinary Education     Hybrid Journal   (Followers: 9)
Equine Veterinary Journal     Hybrid Journal   (Followers: 13)
Ethiopian Veterinary Journal     Open Access   (Followers: 5)
Eurasian Journal of Veterinary Sciences     Open Access   (Followers: 1)
FAVE Sección Ciencias Veterinarias     Open Access  
Folia Veterinaria     Open Access  
Frontiers in Veterinary Science     Open Access   (Followers: 1)
Global Journal of Animal Scientific Research     Open Access   (Followers: 1)
Human & Veterinary Medicine - International Journal of the Bioflux Society     Open Access   (Followers: 5)
ILAR Journal     Hybrid Journal  
In Practice     Full-text available via subscription   (Followers: 2)
Indian Journal of Animal Sciences     Open Access   (Followers: 5)
Indian Journal of Veterinary Anatomy     Full-text available via subscription   (Followers: 4)
Indonesia Medicus Veterinus     Open Access  
Intas Polivet     Full-text available via subscription  
International Journal for Agro Veterinary and Medical Sciences     Open Access   (Followers: 2)
International Journal of Livestock Research     Open Access  
International Journal of Veterinary Science and Medicine     Open Access   (Followers: 4)
Iranian Journal of Applied Animal Science     Open Access  
Irish Veterinary Journal     Open Access   (Followers: 5)
İstanbul Üniversitesi Veteriner Fakültesi Dergisi     Open Access  
Journal of Veterinary Science & Technology     Open Access   (Followers: 6)
Journal of Advanced Veterinary and Animal Research     Open Access   (Followers: 5)
Journal of Animal Behaviour and Biometeorology     Open Access   (Followers: 1)
Journal of Animal Physiology and Animal Nutrition     Hybrid Journal   (Followers: 5)
Journal of Animal Science and Technology     Open Access  
Journal of Applied Animal Nutrition     Hybrid Journal   (Followers: 3)
Journal of Avian Medicine and Surgery     Full-text available via subscription   (Followers: 5)
Journal of Buffalo Science     Hybrid Journal  
Journal of Equine Veterinary Science     Hybrid Journal   (Followers: 11)
Journal of Exotic Pet Medicine     Full-text available via subscription   (Followers: 3)
Journal of Experimental and Applied Animal Sciences     Open Access   (Followers: 1)
Journal of Feline Medicine & Surgery     Hybrid Journal   (Followers: 4)
Journal of Research in Forestry, Wildlife and Environment     Open Access   (Followers: 3)
Journal of Small Animal Practice     Hybrid Journal   (Followers: 11)
Journal of the American Veterinary Medical Association     Full-text available via subscription   (Followers: 28)
Journal of the Selva Andina Research Society     Open Access   (Followers: 1)
Journal of the South African Veterinary Association     Open Access   (Followers: 2)
Journal of Venomous Animals and Toxins     Open Access   (Followers: 4)
Journal of Veterinary Advances     Open Access   (Followers: 4)
Journal of Veterinary Behavior: Clinical Applications and Research     Hybrid Journal   (Followers: 4)
Journal of Veterinary Cardiology     Full-text available via subscription   (Followers: 7)
Journal of Veterinary Diagnostic Investigation     Hybrid Journal   (Followers: 8)
Journal of Veterinary Emergency and Critical Care     Hybrid Journal   (Followers: 15)
Journal of Veterinary Internal Medicine     Open Access   (Followers: 19)
Journal of Veterinary Medical Education     Partially Free   (Followers: 11)
Journal of Veterinary Medicine     Open Access   (Followers: 8)
Journal of Veterinary Medicine and Animal Health     Open Access   (Followers: 4)
Journal of Veterinary Pharmacology and Therapeutics     Hybrid Journal   (Followers: 8)
Journal of Veterinary Research     Open Access  
Journal of Veterinary Science & Medical Diagnosis     Hybrid Journal   (Followers: 4)
Journal of Zoo and Aquarium Research     Open Access   (Followers: 2)
Journal of Zoo and Wildlife Medicine     Full-text available via subscription   (Followers: 6)
Jurnal Agripet     Open Access  
Kenya Veterinarian     Full-text available via subscription   (Followers: 2)
kleintier konkret     Hybrid Journal  
Livestock     Full-text available via subscription   (Followers: 1)
Macedonian Veterinary Review     Open Access   (Followers: 2)
Media Peternakan - Journal of Animal Science and Technology     Open Access   (Followers: 1)
Medical Mycology     Open Access   (Followers: 3)
Medical Mycology Case Reports     Open Access  
Microbes and Health     Open Access   (Followers: 1)
New Zealand Veterinary Journal     Full-text available via subscription   (Followers: 11)
New Zealand Veterinary Nurse     Full-text available via subscription   (Followers: 3)
Nigerian Veterinary Journal     Open Access  
Nutrición Animal Tropical     Open Access   (Followers: 1)
Onderstepoort Journal of Veterinary Research     Open Access   (Followers: 3)
Open Access Animal Physiology     Open Access   (Followers: 4)
Open Journal of Animal Sciences     Open Access   (Followers: 4)
Open Journal of Veterinary Medicine     Open Access   (Followers: 4)
Pesquisa Veterinária Brasileira     Open Access  
pferde spiegel     Hybrid Journal  
Polish Journal of Veterinary Sciences     Open Access   (Followers: 3)
Pratique Médicale et Chirurgicale de l'Animal de Compagnie     Full-text available via subscription  
Preventive Veterinary Medicine     Hybrid Journal   (Followers: 10)
REDVET. Revista Electrónica de Veterinaria     Open Access  
Reproduction in Domestic Animals     Hybrid Journal   (Followers: 1)
Research & Reviews : Journal of Veterinary Science and Technology     Full-text available via subscription   (Followers: 1)
Research in Veterinary Science     Hybrid Journal   (Followers: 10)
Research Journal of Veterinary Sciences     Open Access   (Followers: 9)
Revista Brasileira de Ciência Veterinária     Open Access  
Revista Brasileira de Higiene e Sanidade Animal     Open Access  
Revista Brasileira de Parasitologia Veterinaria     Open Access  
Revista Brasileira de Reprodução Animal     Open Access  
Revista Brasileira de Zootecnia     Open Access   (Followers: 2)
Revista Ciencia Animal     Open Access  
Revista Ciencias Veterinarias     Open Access  
Revista Científica     Open Access  
Revista Colombiana de Ciencias Pecuarias (Colombian journal of animal science and veterinary medicine)     Open Access   (Followers: 1)
Revista Complutense de Ciencias Veterinarias     Open Access  
Revista da Sociedade Brasileira de Ciência em Animais de Laboratório     Open Access  
Revista de Ciências Agroveterinárias     Open Access  
Revista de Educação Continuada em Medicina Veterinária e Zootecnia     Open Access  
Revista de Investigaciones Veterinarias del Perú     Open Access  
Revista de la Facultad de Medicina Veterinaria y de Zootecnia     Open Access   (Followers: 1)
Revista de Medicina Veterinaria     Open Access  
Revista de Salud Animal     Open Access  
Revista Mexicana de Ciencias Pecuarias     Open Access  
Revista MVZ Córdoba     Open Access  
Revista Veterinaria     Open Access  
Revue Marocaine des Sciences Agronomiques et Vétérinaires     Open Access  
SA Stud Breeder / SA Stoetteler     Full-text available via subscription  
Schweizer Archiv für Tierheilkunde     Hybrid Journal  
Scientific Journal of Animal Science     Open Access   (Followers: 3)
Scientific Journal of Veterinary Advances     Open Access   (Followers: 1)
Small Ruminant Research     Hybrid Journal   (Followers: 2)
Sokoto Journal of Veterinary Sciences     Open Access   (Followers: 1)
Spei Domus     Open Access  
Tanzania Veterinary Journal     Full-text available via subscription   (Followers: 1)
team.konkret     Open Access  
The Dairy Mail     Full-text available via subscription   (Followers: 3)
Theriogenology     Hybrid Journal   (Followers: 1)
Topics in Companion Animal Medicine     Hybrid Journal   (Followers: 3)
Transboundary and Emerging Diseases     Hybrid Journal   (Followers: 2)
Trends in Animal and Veterinary Sciences     Open Access   (Followers: 5)
Trends in Parasitology     Full-text available via subscription   (Followers: 8)
Tropical Animal Health and Production     Hybrid Journal  
Tropical Veterinarian     Full-text available via subscription   (Followers: 1)
Turkish Journal of Veterinary and Animal Sciences     Open Access  
veterinär spiegel     Hybrid Journal   (Followers: 1)
Veterinária e Zootecnia     Open Access  
Veterinária em Foco     Open Access  
Veterinaria México     Open Access  
Veterinária Notícias     Open Access  
Veterinary Anaesthesia and Analgesia     Hybrid Journal   (Followers: 11)
Veterinary and Comparative Oncology     Hybrid Journal   (Followers: 7)
Veterinary Clinical Pathology     Hybrid Journal   (Followers: 9)
Veterinary Clinics of North America: Equine Practice     Full-text available via subscription   (Followers: 9)
Veterinary Clinics of North America: Exotic Animal Practice     Full-text available via subscription   (Followers: 7)
Veterinary Clinics of North America: Food Animal Practice     Full-text available via subscription   (Followers: 5)
Veterinary Clinics of North America: Small Animal Practice     Full-text available via subscription   (Followers: 15)
Veterinary Dermatology     Hybrid Journal   (Followers: 7)
Veterinary Immunology and Immunopathology     Hybrid Journal   (Followers: 10)
Veterinary Journal     Hybrid Journal   (Followers: 15)
Veterinary Medicine and Animal Sciences     Open Access   (Followers: 3)
Veterinary Medicine and Science     Open Access  
Veterinary Medicine International     Open Access   (Followers: 7)
Veterinary Medicine: Research and Reports     Open Access   (Followers: 4)
Veterinary Microbiology     Hybrid Journal   (Followers: 8)
Veterinary Nurse     Full-text available via subscription   (Followers: 3)
Veterinary Nursing Journal     Hybrid Journal   (Followers: 3)
Veterinary Ophthalmology     Hybrid Journal   (Followers: 6)
Veterinary Parasitology     Hybrid Journal   (Followers: 9)
Veterinary Pathology     Full-text available via subscription   (Followers: 14)

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Journal Cover Journal of Veterinary Diagnostic Investigation
  [SJR: 0.677]   [H-I: 53]   [8 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1040-6387 - ISSN (Online) 1943-4936
   Published by Sage Publications Homepage  [842 journals]
  • Letter to the Editor
    • Authors: OToole, D; Maxie, G.
      Pages: 196 - 197
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716646369
      Issue No: Vol. 28, No. 3 (2016)
  • Histology and immunohistochemistry of severe inflammatory bowel disease
           versus lymphoma in the ferret (Mustela putorius furo)
    • Authors: Watson, M. K; Cazzini, P, Mayer, J, Gottdenker, N, Reavill, D, Parry, N, Fox, J. G, Sakamoto, K.
      Pages: 198 - 206
      Abstract: Inflammatory bowel disease (IBD) is a common disorder of ferrets (Mustela putorius furo) that may progress to lymphoma. Although routine histology is used to distinguish between these diseases, misclassifications may occur. Immunohistochemistry (IHC) is commonly used to distinguish between IBD and lymphoma in small animals. The objective of our study was to determine the agreement in the diagnosis reached solely using hematoxylin and eosin (HE)-stained, full-thickness sections versus using a combination of HE and IHC. Enteric sections from 44 ferrets previously diagnosed with IBD or intestinal lymphoma and 3 control ferrets were analyzed by pathologists with expertise in ferrets. A pathologist blinded to the original diagnosis assessed the same HE-stained sections. Analysis was then repeated using HE sections in parallel with sections stained using antibodies against CD3 and CD79a. No significant difference was found between the original HE diagnosis and the HE diagnosis reached by the blinded pathologist (p = 0.91) or between the blinded pathologist’s HE versus HE with IHC diagnosis (p = 0.16). In the 2 cases where disagreement was present, IHC was pivotal in reaching a final diagnosis. There was no significant age (p = 0.29) difference between diagnoses; however, significantly more male ferrets were affected with IBD than females (p = 0.004). Immunophenotype of the lymphoma was not correlated with predilection for location in the intestinal wall (p = 0.44). Results suggest that although IHC is not necessary to distinguish IBD from intestinal lymphoma in ferrets, it can be useful a definitive diagnosis in cases of severe IBD.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716641156
      Issue No: Vol. 28, No. 3 (2016)
  • Detection of pregnancy-associated glycoproteins in milk and blood as a
           test for early pregnancy in dairy cows
    • Authors: Commun, L; Velek, K, Barbry, J.-B, Pun, S, Rice, A, Mestek, A, Egli, C, Leterme, S.
      Pages: 207 - 213
      Abstract: The objective of our study was to evaluate 2 pregnancy-associated glycoprotein (PAG)-based enzyme-linked immunosorbent assays (ELISAs) for use with either blood or milk. From 12 dairy farms, 116 Montbéliarde or Holstein cows were selected that had either undergone artificial insemination (AI; n = 102) or had calved (n = 14) 2–3 months earlier and had not undergone any further AI. Serum, plasma, and milk were obtained from all cows; serum and plasma were analyzed using the blood pregnancy test and milk using the milk pregnancy test. No false-positive results were observed when samples of the 14 noninseminated cows were tested. Cows undergoing AI were sampled at ~16, 30, and 41 days post-AI. An additional milk sample was taken at ~53 days post-AI. To establish whether the inseminated cows were pregnant, the cows were subjected to transrectal ultrasonography (TU) on or around day 41. Of the 102 inseminated cows, 63 were confirmed pregnant by TU. By day 30, the serum, plasma, and milk ELISAs demonstrated 100%, 100%, and 98.1% sensitivity and 88.6%, 88.9%, and 90.3% specificity, respectively, with potential pregnancy losses 30–41 days post-AI. Accuracy obtained on serum, plasma, and milk at ~41 days post-AI and on milk at ~53 days post-AI ranged from 97.4% to 100%. There were no differences of practical significance in performance between the blood and milk ELISAs for the sampling dates chosen. This new diagnostic capability with milk samples offers a major improvement in bovine reproductive management.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716632815
      Issue No: Vol. 28, No. 3 (2016)
  • A rapid cycleave PCR method for distinguishing the vaccine strain Brucella
           abortus A19 in China
    • Authors: Nan, W; Zhang, Y, Tan, P, Xu, Z, Chen, Y, Mao, K, Chen, Y.
      Pages: 214 - 218
      Abstract: Brucellosis is a widespread zoonotic disease caused by Brucella spp. Immunization with attenuated vaccines has proved to be an effective method of prevention; however, it may also interfere with diagnosis. Brucella abortus strain A19, which is homologous to B. abortus strain S19, is widely used for the prevention of bovine brucellosis in China. For effective monitoring of the control of brucellosis, it is essential to distinguish A19 from field strains. Single-nucleotide polymorphism–based assays offer a new approach to such discrimination studies. In the current study, we developed a cycleave PCR assay that successfully distinguished attenuated vaccine strains A19 and S19 from 22 strains of B. abortus and 57 strains of 5 other Brucella species. The assay gave a negative reaction with 4 non-Brucella species. The minimum sensitivity of the assay, evaluated using 10-fold dilutions of chromosomal DNA, was 7.6 fg for the A19 strain and 220 fg for the single non-A19/non-S19 Brucella strain tested (B. abortus 104M). The assay was also reproducible (intra- and interassay coefficients of variation: 0.003–0.01 and 0.004–0.025, respectively). The cycleave assay gave an A19/S19-specific reaction in 3 out of 125 field serum samples, with the same 3 samples being positive in an alternative A19/S19-specific molecular assay. The cycleave assay gave a total of 102 Brucella-specific reactions (3 being the A19/S19-specific reactions), whereas an alternative Brucella-specific assay gave 92 positive reactions (all also positive in the cycleave assay). Therefore, this assay represents a simple, rapid, sensitive, and specific tool for use in brucellosis control.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716640315
      Issue No: Vol. 28, No. 3 (2016)
  • Phylogenetic characterization of a novel herpesvirus found in the liver
           and lungs of a Chilean flamingo (Phoenicopterus chilensis)
    • Authors: Coverdill, C. C; Barnes, J. A, Garner, M. M, Hinton, K. L, Childress, A. L, Wellehan, J. F. X.
      Pages: 219 - 224
      Abstract: A novel herpesvirus was detected in a 17-day-old Chilean flamingo (Phoenicopterus chilensis) with pneumonia, hepatopathy, and severe anemia that was housed in California. Postmortem examination identified a pale, enlarged liver, mildly increased fluid in the lungs, and red foci in the spleen. Histologic examination revealed marked hepatic necrosis with syncytia, splenic necrosis, and interstitial pneumonia with eosinophilic intranuclear inclusions within hepatocytes and in unidentified cells of the lung. Transmission electron microscopy identified virions consistent with a herpesvirus in the nucleus and cytoplasm of degenerative hepatocytes. Nested consensus PCR, sequencing, and phylogenetic analysis identified a novel herpesvirus within the genus Iltovirus in the subfamily Alphaherpesvirinae.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716641157
      Issue No: Vol. 28, No. 3 (2016)
  • Helicobacter bilis and Helicobacter trogontum: infectious causes of
           abortion in sheep
    • Authors: Gill, J; Haydon, T. G, Rawdon, T. G, McFadden, A. M. J, Ha, H.-J, Shen, Z, Feng, Y, Pang, J, Swennes, A. G, Paster, B. J, Dewhirst, F. E, Fox, J. G, Spence, R. P.
      Pages: 225 - 234
      Abstract: The aim of our study was to determine the association of Helicobacter spp. that had flexispira morphology with ovine abortion, and to understand the importance of these organisms as a cause of ovine abortion in New Zealand. A retrospective diagnostic survey was carried out on laboratory submissions from ovine abortion outbreaks. A comparison was made of the proportion of laboratory submissions where Helicobacter spp. were detected from flocks that had no other agent identified (group A) with a group that had a known cause of abortion identified (group B). This latter group was considered to be a negative control, given the premise that Helicobacter spp. were not causing abortions and that Helicobacter spp. should be present at a lower rate in the group. Where no diagnosis had been made, aborted material was positive for Helicobacter spp. with flexispira morphology in 8 submissions (20%, 8/40) from 5 of the 31 survey farms (16%, 5/31). Helicobacter spp. were not detected in any of the 18 submissions from the 17 control farms (group B). Helicobacter spp. were confirmed by 16S ribosomal RNA sequencing of 3 of the Helicobacter spp. isolated by culture from the livers of aborted sheep fetuses, and 7 of the 8 where samples were positive in a Helicobacter PCR assay. The Helicobacter spp. were identified as Helicobacter trogontum (Flexispira taxon 5 genotype) and Helicobacter bilis (Flexispira taxon 8 genotype). The findings support Helicobacter spp. being a probable causative agent of ovine abortions in New Zealand.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716638704
      Issue No: Vol. 28, No. 3 (2016)
  • Validation of an enzyme-linked immunosorbent assay for measurement of
           feline haptoglobin
    • Authors: Stiller, J; Jasensky, A.-K, Hennies, M, Einspanier, R, Kohn, B.
      Pages: 235 - 243
      Abstract: Haptoglobin is a positive moderate acute phase protein (APP) in cats. Measurement of haptoglobin can be used in the diagnosis, prognosis, and monitoring of systemic inflammatory disease, especially by creating profiles with major APPs. The aim of our study was to validate a sandwich enzyme-linked immunosorbent assay (ELISA) for measurement of feline haptoglobin. The validation included an assessment of precision, accuracy, detection limit, method comparison with a spectrophotometric assay, and evaluation of the overlap performance. The concentration of haptoglobin was measured in serum from 27 healthy and 23 sick cats. The coefficients of variation were 2.5–4.7% for intra-assay variability and 7.1–11.6% for interassay variability. The ratio of observed to expected dilutional parallelism of 4 serum samples was 108.1–118.4%. The ratio of observed to expected spike recovery of 4 serum samples was 90.8–94.0%. The lower detection limit was 0.19 g/L. Method comparison revealed a positive correlation (r s = 0.949, P < 0.0001) and a proportional bias between the methods of –38.9%. Agreement between the methods was not clinically acceptable. Overlap performance of the ELISA was deemed satisfactory. The sandwich ELISA measures feline haptoglobin with an analytical and overlap performance acceptable for clinical purposes. Given the observed bias, the ELISA cannot be used interchangeably with the spectrophotometric assay.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716634397
      Issue No: Vol. 28, No. 3 (2016)
  • Pathology of ear hematomas in swine
    • Authors: Drolet, R; Helie, P, DAllaire, S.
      Pages: 244 - 248
      Abstract: The objectives of our study were to describe the pathology of ear hematomas in swine and to add to the comprehension of the pathogenesis of this condition. The pathogenesis of aural hematomas has been studied mainly in dogs; however, disagreements exist about the precise anatomic location of the hemorrhage. Sixteen pigs with ear hematoma at various stages of development were included in this study. The pigs were submitted for routine autopsy for various and unrelated reasons over a period of several years. Based on gross examination, the 16 cases of aural hematomas were subjectively classified as acute (n = 6), subacute (n = 3), and chronic (n = 7). The age of the animals at the time of autopsy ranged from 2 weeks to adulthood, with all acute cases being
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716630768
      Issue No: Vol. 28, No. 3 (2016)
  • Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and
           surfactant protein in the lungs of sheep with pulmonary adenomatosis
    • Pages: 249 - 256
      Abstract: Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring cancer in sheep that is caused by the Jaagsiekte sheep retrovirus (JSRV). Because the pathologic and epidemiologic features of OPA are similar to those of bronchoalveolar carcinoma in humans, OPA is considered a useful animal model for pulmonary carcinogenesis. In this study, 3,512 lungs from various breeds of sheep were collected and macroscopically examined. OPA was identified in 30 sheep, and samples of these animals were further examined by histologic, immunohistochemical (p53 protein, surfactant protein A [SP-A], proliferating cell nuclear antigen [PCNA], JSRV matrix protein [MA]), and PCR methods. Papillary or acinar adenocarcinomas were detected microscopically in the affected areas. Immunoreactivity for p53 PAb240 was detected in 13 sheep, whereas p53 DO-1 was not detected in any of the OPA animals. PCNA immunoreactivity was recorded in 27 animals. SP-A and JSRV MA protein was immunopositive in all 30. JSRV proviral DNA was detected by PCR analysis in all of the lung samples collected from OPA animals. In addition, the pulmonary SP-A levels were increased in tumor cells. The results of this study suggest that PCNA and p53 protein expression may be useful indicators in monitoring malignancy of pulmonary tumors.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716636939
      Issue No: Vol. 28, No. 3 (2016)
  • Establishment of a quantitative real-time PCR for the detection of
           Pneumocystis carinii f. sp. suis in bronchoalveolar lavage samples from
    • Pages: 257 - 262
      Abstract: Respiratory diseases in pigs are mostly polymicrobial, and the involved pathogens can vary from farm to farm. The impact of Pneumocystis carinii (P. c.) f. sp. suis on respiratory disorders has not been comprehensively appraised because tests were limited to lung tissue samples and, for this reason, it was not possible to detect the fungus in living animals. In the present study, bronchoalveolar lavage fluid (BALF) from 12 pigs and oral fluid samples from 9 pigs were tested for the presence of Pneumocystis by quantitative real-time PCR. The results from these 2 clinical specimens were compared with Pneumocystis quantities in lung tissue samples. Pneumocystis quantities in BALF correlated significantly to those in lung tissue. BALF has proved to be an adequate specimen for detection of various respiratory pathogens in pigs, and the collection procedure directly on farms is also well established. In contrast to the BALF results, all oral fluid samples were negative. Thus, specimens from the lower respiratory tract should generally be preferred for the detection of Pneumocystis. Additionally, under farm conditions, oral fluid is mainly collected in the form of collective samples per pen. In the present study, oral swab sampling of individual pigs was intended but failed in 3 of 12 pigs because they did not salivate sufficiently. As a conclusion, only BALF can be recommended as a useful tool for Pneumocystis herd monitoring in vivo.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716641158
      Issue No: Vol. 28, No. 3 (2016)
  • Cysticercus fasciolaris infection in wild rats (Rattus norvegicus) in
           Korea and formation of cysts by remodeling of collagen fibers
    • Authors: Lee, B.-W; Jeon, B.-S, Kim, H.-S, Kim, H.-C, Yoon, B.-I.
      Pages: 263 - 270
      Abstract: Cysticercus fasciolaris, the larval form of Taenia taeniaeformis, is commonly encountered in rodents. In our study, 287 wild rats (Rattus norvegicus) in South Korea were examined in 2010 and 2011. Of 287 rats, 97 (33.8%) were infected with C. fasciolaris. A strong positive correlation was found between the host body weight and prevalence in both sexes, regardless of the year of collection. The liver was the most common habitat of the parasite, and the lung was the most frequent ectopic region, followed by mesentery, pleura, abdominal wall, and kidney. The lesions of the affected organs were generally characterized by well-developed cysts, each containing a larva. However, the cysts within kidney and abdominal wall were poorly organized, filled with abscess, and lacked larvae. Collagen types I and III, but not type IV, played significant roles in constructing the cysts at differential stages, addressed by immunohistochemistry. During cyst wall development, both collagen types contributed equally to cyst formation at the early stage, whereas collagen type I was the major component at the late stage (p < 0.05). In early-stage cysts, distribution of collagens was interestingly differential depending on the development stage, as collagen type I was localized in the outer layer and type III was located in the inner layer. Our results suggest that an appropriate remodeling process of collagen fibers is necessary for C. fasciolaris to build the well-conditioned cysts in the target organs for survival.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716643129
      Issue No: Vol. 28, No. 3 (2016)
  • Expression of serotonin, chromogranin-A, serotonin receptor-2B, tryptophan
           hydroxylase-1, and serotonin reuptake transporter in the intestine of dogs
           with chronic enteropathy
    • Authors: Bailey, C; Ruaux, C, Stang, B. V, Valentine, B. A.
      Pages: 271 - 278
      Abstract: Serotonin regulates many intestinal motor and sensory functions. Altered serotonergic metabolism has been described in human gastrointestinal diseases. The objective of our study was to compare expression of several components of the serotonergic system [serotonin (5-HT), serotonin reuptake transporter protein (SERT), tryptophan hydroxylase-1 (TPH-1), 5-HT receptor2B (5-HT2B)] and the enterochromaffin cell marker chromogranin-A (CgA) in the intestinal mucosa between dogs with chronic enteropathy and healthy controls. Serotonin and CgA expression were determined by immunohistochemistry using banked and prospectively obtained, paraffin-embedded canine gastrointestinal biopsies (n = 11), and compared to a control group of canine small intestinal sections (n = 10). Expression of SERT, TPH-1, and 5-HT2B were determined via real-time reverse transcription (qRT)-PCR using prospectively collected endoscopic duodenal biopsies (n = 10) and compared to an additional control group of control duodenal biopsies (n = 8, control group 2) showing no evidence of intestinal inflammation. Dogs with chronic enteropathies showed strong staining for both 5-HT and CgA. Mean positive cells per high power field (HPF) were significantly increased for both compounds in dogs with chronic enteropathies (p < 0.001 for 5-HT; p < 0.05 for CgA). The number of 5-HT–positive and CgA-positive cells/HPF showed significant correlation in the entire group of dogs, including both diseased and healthy individuals (Pearson r 2 = 0.2433, p = 0.016). No significant differences were observed for SERT, TPH-1, or 5-HT2B expression; however, dogs with chronic enteropathy showed greater variability in expression of TPH-1 and 5-HT2B. We conclude that components of the neuroendocrine system show altered expression in the intestinal mucosa of dogs with chronic enteropathy. These changes may contribute to nociception and clinical signs in these patients.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638715618232
      Issue No: Vol. 28, No. 3 (2016)
  • Diagnostic investigation of new disease syndromes in farmed Australian
           saltwater crocodiles (Crocodylus porosus) reveals associations with
           herpesviral infection
    • Authors: Shilton, C. M; Jerrett, I. V, Davis, S, Walsh, S, Benedict, S, Isberg, S. R, Webb, G. J. W, Manolis, C, Hyndman, T. H, Phalen, D, Brown, G. P, Melville, L.
      Pages: 279 - 290
      Abstract: Since 2006, 3 new disease syndromes have emerged in farmed saltwater crocodiles (Crocodylus porosus) in the Northern Territory of Australia. We describe the syndromes through a retrospective study of laboratory findings from 187 diagnostic cases submitted to Berrimah Veterinary Laboratories between 2005 and 2014. The first syndrome was characterized by conjunctivitis and/or pharyngitis (CP), primarily in hatchlings. Herpesviruses were isolated in primary crocodile cell culture, or were detected by PCR directly from conjunctiva or pharyngeal tissue, in 21 of 39 cases of CP (54%), compared with 9 of 64 crocodiles without the syndrome (14%, p < 0.0001). Chlamydiaceae were detected by PCR in conjunctiva or pharyngeal tissue of 55% of 29 CP cases tested, and of these, 81% also contained herpesvirus. The second syndrome occurred in juveniles and growers exhibiting poor growth, and was characterized histologically by systemic lymphoid proliferation and nonsuppurative encephalitis (SLPE). Herpesviruses were isolated or detected by PCR from at least 1 internal organ in 31 of 33 SLPE cases (94%) compared with 5 of 95 crocodiles without the syndrome (5%, p < 0.0001). The third syndrome, characterized by multifocal lymphohistiocytic infiltration of the dermis (LNS), occurred in 6 harvest-sized crocodiles. Herpesviruses were isolated from at least 1 skin lesion in 4 of these 6 cases. Although our study revealed strong associations between herpesvirus and the CP and SLPE syndromes, the precise nature of the role of herpesvirus, along with the pathogenesis and epidemiology of the syndromes, requires further investigation.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716642268
      Issue No: Vol. 28, No. 3 (2016)
  • Bovine mastitis outbreak in Japan caused by methicillin-resistant
           Staphylococcus aureus New York/Japan clone
    • Authors: Hata; E.
      Pages: 291 - 298
      Abstract: Many methicillin-resistant Staphylococcus aureus (MRSA) strains are multidrug-resistant; consequently, infectious diseases involving MRSA are recognized as troublesome diseases not only in human health care but also in animal health care. A bovine mastitis case caused by MRSA isolates of the New York/Japan clone (NJC), which occurred in Japan in 2005, was monitored in the current study. Isolates of the NJC are typical of hospital-acquired MRSA in Japan. The genetic backgrounds of these strains differ from those of bovine-associated S. aureus, which are typically of clonal complex (CC)97, CC705, and CC133. Moreover, the NJC isolates in this bovine outbreak possessed a β-hemolysin–converting bacteriophage and an immune evasion cluster, as found in the NJC isolates from humans, so it is possible that this clone was introduced into the dairy herd by a human carrier. Most bovine intramammary infections (IMIs) caused by the NJC isolates in our study were asymptomatic, and obvious clinical signs were recognized in only the first 3 infected cows. Of a total of 78 cows, 31 cows were MRSA carriers, and these carrier cows were detected by testing the milk of all lactating cows at 1-month intervals. These S. aureus carrier cows were culled or the infected quarter was dried off and no longer milked. Both IMI and mastitis caused by MRSA were completely eradicated after 5 months. Genotyping data suggested that exchanging of the staphylococcal cassette chromosome mec (the determining factor in methicillin resistance) occurred easily between MRSA and methicillin-sensitive S. aureus in the udders of carrier cows. This case study demonstrates an effective procedure against the spread of MRSA in a dairy herd, and highlights the risk of emergence of new MRSA strains in a dairy herd.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716643126
      Issue No: Vol. 28, No. 3 (2016)
  • Development of a duplex rapid assay for immunoglobulins M and G to
           evaluate the parvoviral immune status of clinically healthy dogs
    • Authors: Palma, M; de la Roja, N, Monton, M, Sastre, P, Ramirez, S, Barreiro, B, Venteo, A, Rueda, P.
      Pages: 299 - 303
      Abstract: A duplex rapid assay for detection of serum antibodies to canine parvovirus (CPV) was developed. Canine immunoglobulin (Ig)M or IgG were captured in immunotubes with anti-canine IgM or IgG and detected with parvovirus VP2 recombinant protein followed by an anti-VP2 monoclonal antibody. The assay was tested using a collection of sera from dogs that were vaccinated against CPV on arrival at an animal shelter in Madrid, Spain. Results were compared with those of 2 commercial enzyme-linked immunosorbent assays (ELISAs) considered as reference techniques. A high correlation was found between the duplex rapid assay and the ELISAs, presenting an accuracy of 98% and 100% for IgG and IgM, respectively. According to the IgG and IgM levels at days 0–3 postvaccination, the samples were divided into 2 groups. One group of dogs showed high IgG and low IgM values at the first sampling post-vaccination and during the following 14 days, indicating that they had previously been in contact with the virus, either by vaccination or infection before arrival at the animal shelter. A second group of dogs appeared to be unvaccinated or uninfected before arrival at the animal shelter because they had negative IgM and IgG values soon after vaccination. These animals responded to vaccination, as demonstrated by seroconversion of both isotypes of immunoglobulins. The developed assay appears to be useful in determining the unknown immune status of dogs to CPV, especially in kennels and shelters where the rate of infection by CPV is relatively high.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638716634400
      Issue No: Vol. 28, No. 3 (2016)
  • Retrospective study of reticulocyte indices as indicators of
           iron-restricted erythropoiesis in dogs with immune-mediated hemolytic
    • Authors: Schaefer, D. M. W; Stokol, T.
      Pages: 304 - 308
      Abstract: Iron-restricted erythropoiesis can occur as a result of an absolute deficiency of iron stores, inflammation-mediated iron sequestration, or functional iron deficiency (in which release of stored iron is slower than the iron uptake by erythroid precursors during intense erythropoiesis). Reticulocyte indices are used to identify iron-restricted erythropoiesis, with the reticulocyte hemoglobin content (CHr) being the most commonly used index in human patients. Dogs with immune-mediated hemolytic anemia (IMHA) may have iron-restricted erythropoiesis caused by inflammation-mediated iron sequestration and/or functional iron deficiency, which could contribute to anemia severity and blunt the regenerative response in some dogs. To investigate this possibility, reticulocyte indices were examined retrospectively in 14 dogs (2–15 years of age; 9 spayed females, 1 intact female, 4 neutered males) with IMHA, and no clinical evidence of blood loss was found to suggest absolute iron deficiency. Five dogs (34%) had CHr below the preestablished lower reference limit (24.5 pg), and hematocrit was significantly lower in these dogs (p = 0.042, nonpaired t-test). Our results suggest that some dogs with IMHA may have iron-restricted erythropoiesis as a result of functional iron deficiency, inflammation-mediated iron sequestration, or (less likely) absolute iron deficiency. Further study is warranted to evaluate if dogs with IMHA may benefit from parenteral iron therapy.
      PubDate: 2016-05-02T14:03:39-07:00
      DOI: 10.1177/1040638715618231
      Issue No: Vol. 28, No. 3 (2016)
  • Development of a consensus reverse transcription PCR assay for the
           specific detection of tortoise picornaviruses
    • Authors: Marschang, R. E; Ihasz, K, Kugler, R, Lengyel, G, Feher, E, Marton, S, Banyai, K, Aqrawi, T, Farkas, S. L.
      Pages: 309 - 314
      Abstract: Picornaviruses (PVs) of different terrestrial tortoise species, previously designated as Virus "X," have been frequently detected from various tissues by virus isolation in Terrapene heart cell culture as the preferred laboratory method for diagnosis. Here, we describe the development of 2 diagnostic reverse transcription (RT)-PCR–based assays for the identification and characterization of tortoise PVs belonging to the tentative genus Topivirus. To test the novel diagnostic systems, PVs were isolated from swab and tissue samples collected in Germany, Italy, and Hungary between 2000 and 2013. All 25 tested isolates gave positive results with both novel consensus primer sets. Sequencing of the amplified products confirmed that all studied viruses were members of the new proposed genus Topivirus. Phylogenetic analyses clearly distinguished 2 lineages within the genus. Based on sequence analysis, no association was observed between the geographic distribution and genetic relatedness. Furthermore, no strict host specificity was indicated. The PCR-based diagnosis may provide a time-saving and sensitive method to detect tortoise PVs, and evaluation of PV presence in these animals may help control virus spread.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716628584
      Issue No: Vol. 28, No. 3 (2016)
  • Renal leiomyosarcoma in a cat
    • Authors: Evans, D; Fowlkes, N.
      Pages: 315 - 318
      Abstract: Renal leiomyosarcoma was diagnosed in a 10-year-old Domestic Shorthair cat with a 3-year history of clinically managed, chronic renal disease. Sudden death was preceded by a brief episode of mental dullness and confusion. At postmortem examination, the gross appearance of the left kidney was suggestive of hydronephrosis, and a nephrolith was present in the contralateral kidney. However, histology revealed an infiltrative, poorly differentiated, spindle cell sarcoma bordering the grossly cavitated area. Neoplastic cells were immunoreactive for vimentin and smooth muscle actin, which led to a diagnosis of renal leiomyosarcoma; neoplastic cells were not immunoreactive for desmin. Leiomyosarcoma arising in the kidney is a rare occurrence in humans and an even rarer occurrence in veterinary medicine with no prior cases being reported in cats in the English literature. The macroscopic appearance of the tumor at postmortem examination was misleadingly suggestive of hydronephrosis as a result of the large cavitation and may be similar to particularly unusual cases of renal leiomyosarcomas in humans that have a cystic or cavitated appearance.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716638126
      Issue No: Vol. 28, No. 3 (2016)
  • Dermatomycosis in three central bearded dragons (Pogona vitticeps)
           associated with Nannizziopsis chlamydospora
    • Authors: Schmidt-Ukaj, S; Loncaric, I, Spergser, J, Richter, B, Hochleithner, M.
      Pages: 319 - 322
      Abstract: Chronic dermatomycosis was identified in 3 central bearded dragons (Pogona vitticeps), held as companion animals by the same owner. Clinical signs of dermatomycosis included subcutaneous masses as well as crusty, erosive, and ulcerative skin lesions. The facial region was affected in 2 of the 3 cases. Masses were surgically excised, and histology confirmed necrotizing and granulomatous inflammatory processes associated with fungal hyphae. Two of the bearded dragons were euthanized because of their deteriorating condition. In both cases, postmortem histology confirmed systemic fungal infections despite treatment of 1 animal with itraconazole. In the third bearded dragon, therapy with voriconazole at 10 mg/kg was initially effective, but mycotic lesions reappeared 15 months later. Nannizziopsis chlamydospora was identified by PCR and subsequent DNA sequencing in 2 of these cases.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716636422
      Issue No: Vol. 28, No. 3 (2016)
  • Pyrosequencing as a fast and reliable tool to determine clade affiliation
           for equine Influenza A virus
    • Authors: Bernardino, P. N; Mapes, S. M, Corbin, R, Pusterla, N.
      Pages: 323 - 326
      Abstract: The objective of our study was to determine the clade affiliation of 116 contemporary equine Influenza A virus (EIV) isolates using pyrosequencing. The EIV isolates originated from horses with clinical signs of equine influenza and laboratory confirmation of EIV by real-time quantitative PCR (qPCR) in nasal secretions. Clade affiliation was performed on the basis of a single nucleotide polymorphism at 2 positions of the hemagglutinin 1 gene. Pyrosequencing was able to clearly classify EIV Florida sublineage prototype A/equine/Ohio/1/2003 and prototype A/equine/Richmond/1/2007 as clade 1 and 2, respectively. Out of the 116 EIV qPCR-positive samples, 113 (97.4%) were classified as belonging to clade 1 Florida sublineage, whereas 3 (2.6%) were classified as clade 2. All clade 1 EIV strains were detected in domestic horses, whereas the 3 clade 2 EIV strains originated from horses recently imported to the United States. Although clade 1 EIV strains are endemic in the United States, international transportation of horses represents a real risk in introducing clade 2 EIV strains into North America.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716638123
      Issue No: Vol. 28, No. 3 (2016)
  • Cutaneous epitheliotropic T-cell lymphoma with systemic dissemination in a
    • Authors: Mineshige, T; Kawarai, S, Yauchi, T, Segawa, K, Neo, S, Sugahara, G, Kamiie, J, Hisasue, M, Shirota, K.
      Pages: 327 - 331
      Abstract: Cutaneous epitheliotropic T-cell lymphoma (CETL) is characterized by neoplastic T-cell infiltration of the epidermis, adnexal structures, and oral mucosa. The objective of this report was to describe the pathological findings of a canine case of terminal-stage CETL. A 10-year-old, mixed-breed, neutered male dog was presented with erosion of the oral mucosa and mucocutaneous junction. The dog was diagnosed with CETL with no evidence of metastasis. Despite chemotherapy, the dog was re-presented with oral pain, vomiting, and diarrhea, and died 17 months after the first visit to the hospital. A complete autopsy was performed. Histologic examination of the primary lesion and systemic organs was performed. Gross examination revealed an advanced-stage oral lesion. Distinct tumor formation was not observed in the primary sites and systemic organs. Histologically, the primary oral lesion was characterized by massive intraepithelial infiltration of a large number of neoplastic lymphocytes. The neoplastic cells in the metastatic sites also showed exclusive epitheliotropic proliferation in organs, including the trachea, tonsils, esophagus, stomach, small intestine, colon, anal mucosa, liver, pancreas, kidneys, urinary bladder, prostate gland, ear canals, and auricular and ventral skin. Immunohistochemically, the neoplastic cells were positive for CD3 and negative for CD20 as well as CD79α, supporting a diagnosis of CETL with systemic dissemination. In canine CETL with systemic signs, systemic metastasis should be considered even without evident mass formation. Neoplastic lymphocytes of CETL showed distinct epitheliotropism even in the systemic metastatic sites.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716637642
      Issue No: Vol. 28, No. 3 (2016)
  • The use of native fluorescence analysis of synovial fluid in the diagnosis
           of medial compartment disease in medium- and large-breed dogs
    • Authors: Bilska, K; Steffekova, Z, Birkova, A, Marekova, M, Ledecky, V, Hluchy, M, Kiskova, T.
      Pages: 332 - 337
      Abstract: We assumed that proteins are most likely responsible for synovial fluid fluorescence and that changes detected in fluorescence intensity are most likely the result of changes in the concentration of fluorescent proteins. Synchronous fluorescent matrices from synovial fluid samples were measured in the excitation wavelength range of 200–350 nm using a luminescence spectrophotometer. The synchronous matrix of synovial fluid consists of 2 dominant fluorescent centers (F1 and F2) in the ultraviolet region. The fluorescence intensities of both centers were significantly higher in pathological samples, with p = 0.001 (a 59% increase of the median value) for the F1 center and p = 0.002 (a 52% increase of the median value) for the F2 center. Receiver operating characteristic analysis confirmed that synovial fluid autofluorescence is a significant predictor of medial compartment disease in dogs, with the area under the curve at 0.776 (F1) and 0.778 (F2). We did not detect any differences in the autofluorescence of synovial fluid between male and female, or any breed-based changes. No position changes of fluorescent centers were recorded in the synovial fluid in diseased dogs compared with healthy dogs. The synovial fluid metabolic fingerprint of canine patients with medial compartment disease differed from that of healthy dogs. Our study demonstrated the feasibility of synovial fluid fingerprinting to identify disease-specific profiles of synovial fluid metabolites.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716640312
      Issue No: Vol. 28, No. 3 (2016)
  • Histologic and molecular characterization of Edwardsiella piscicida
           infection in largemouth bass (Micropterus salmoides)
    • Authors: Fogelson, S. B; Petty, B. D, Reichley, S. R, Ware, C, Bowser, P. R, Crim, M. J, Getchell, R. G, Sams, K. L, Marquis, H, Griffin, M. J.
      Pages: 338 - 344
      Abstract: The genus Edwardsiella is composed of a diverse group of facultative anaerobic, gram-negative bacteria that can produce disease in a wide variety of hosts, including birds, reptiles, mammals, and fish. Our report describes the isolation and identification of Edwardsiella piscicida associated with chronic mortality events in 2 separate captive largemouth bass (Micropterus salmoides) populations in New York and Florida. Wet-mount biopsies of skin mucus, gill, kidney, and spleen from several affected largemouth bass contained significant numbers of motile bacteria. Histologic examination revealed multifocal areas of necrosis scattered throughout the heart, liver, anterior kidney, posterior kidney, and spleen. Many of the necrotic foci were encapsulated or replaced by discrete granulomas and associated with colonies of gram-negative bacteria. Initial phenotypic and matrix-assisted laser desorption ionization–time of flight mass spectrometric analysis against existing spectral databases of recovered isolates identified these bacteria as Edwardsiella tarda. Subsequent molecular analysis using repetitive sequence mediated and species-specific PCR, as well as 16S rRNA, rpoB, and gyrB sequences, classified these isolates as E. piscicida. As a newly designated taxon, E. piscicida should be considered as a differential for multiorgan necrosis and granulomas in largemouth bass.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716637639
      Issue No: Vol. 28, No. 3 (2016)
  • Pretreatment of serum samples to reduce interference of colostrum-derived
           specific antibodies with detection of Bovine viral diarrhea virus antigen
           by ELISA in young calves
    • Authors: Lanyon, S. R; Reichel, M. P.
      Pages: 345 - 349
      Abstract: Antigen enzyme-linked immunosorbent assay (ELISA) is used for the detection of Bovine viral diarrhea virus persistently infected (BVDV PI) cattle; however, colostrum-derived antibodies may interfere with antigen detection in serum from young PI calves. Our study aimed to assess serum pretreatment methods for reducing such interference. Dilution of PI serum with serum containing specific antibody showed that antibody levels equivalent to those observed in colostrum-fed calves were able to eliminate all antigen signals in a serum sample. Serum was treated with ethylenediamine tetra-acetic acid at pH 4.5, 5.5, 6.5, and 7.5, then boiled, centrifuged, and the supernatant-recovered. BVDV antibody was undetectable by ELISA in supernatants from treated samples, and the antigen ELISA signal was improved. Maximum antigen signal recovery of >90% was achieved at pH 5 ± 0.5. When this optimal treatment method was applied to field samples from 3 PI calves (which were negative in the antigen-capture ELISA without treatment), the antigen signal improved and gave a positive result in each case. Pretreatment may provide an improvement in the detection of young PI calves.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716633496
      Issue No: Vol. 28, No. 3 (2016)
  • Systemic Trichosporon loubieri infection in a cat
    • Authors: Rissi, D. R; Kirby, K. D, Sanchez, S.
      Pages: 350 - 353
      Abstract: Our study describes a case of systemic Trichosporon loubieri infection in a cat with acute dyspnea, anorexia, and aggressiveness. Physical examination revealed multiple ulcerative cutaneous lesions on the abdomen, neck, and thorax. Thoracic radiographs and ultrasound showed multiple mediastinal nodules and marked pleural effusion, respectively. A cutaneous biopsy from the ulcerated wounds revealed necrogranulomatous dermatitis and panniculitis with numerous intralesional fungal hyphae. Fungal culture on fresh swab samples from the cutaneous lesions yielded growth of a fungal organism that was further identified as Trichosporon loubieri by PCR and DNA sequencing. The cat was subsequently euthanized and submitted to autopsy. Gross pathology changes consisted of multifocal to coalescing white nodules ranging from 5 to 10 mm in diameter that expanded the mediastinal fat, intrathoracic lymph nodes, lungs, and costal pleura. These lesions consisted of areas of necrogranulomatous inflammation with numerous intralesional fungal hyphae morphologically similar to those observed in the cutaneous biopsy sample. Gross and histologic changes were consistent with a systemic fungal infection, and the etiologic diagnosis was supported by fungal culture. Fungal identity was confirmed by DNA sequencing of D1-D2 and TS1 regions.
      PubDate: 2016-05-02T14:03:40-07:00
      DOI: 10.1177/1040638716640313
      Issue No: Vol. 28, No. 3 (2016)
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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