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  Subjects -> VETERINARY SCIENCE (Total: 206 journals)
Acta Scientiae Veterinariae     Open Access  
Acta Veterinaria     Open Access   (Followers: 1)
Acta Veterinaria Brasilica     Open Access  
Acta Veterinaria Hungarica     Full-text available via subscription   (Followers: 1)
Acta Veterinaria Scandinavica     Open Access   (Followers: 1)
Advances in Animal Biosciences     Full-text available via subscription   (Followers: 6)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 7)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
African Journal of Wildlife Research     Full-text available via subscription  
Alexandria Journal of Veterinary Sciences     Open Access  
American Journal of Animal and Veterinary Sciences     Open Access   (Followers: 10)
American Journal of Primatology     Hybrid Journal   (Followers: 7)
American Journal of Veterinary Research     Full-text available via subscription   (Followers: 18)
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C     Hybrid Journal   (Followers: 2)
Animal Behaviour     Hybrid Journal   (Followers: 151)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5)
Animal Health Research Reviews     Hybrid Journal   (Followers: 4)
Animal Nutrition     Open Access   (Followers: 5)
Animal Reproduction     Open Access  
Animal Reproduction Science     Hybrid Journal   (Followers: 6)
Animals     Open Access   (Followers: 6)
Annals of Agricultural and Environmental Medicine     Open Access   (Followers: 1)
Annual Review of Animal Biosciences     Full-text available via subscription   (Followers: 4)
Anthrozoos : A Multidisciplinary Journal of The Interactions of People & Animals     Hybrid Journal   (Followers: 8)
Archives of Animal Nutrition     Hybrid Journal   (Followers: 7)
Archivos de Medicina Veterinaria     Open Access   (Followers: 1)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access   (Followers: 1)
Arquivos de Ciências Veterinárias e Zoologia da UNIPAR     Open Access  
Ars Veterinaria     Open Access  
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atatürk Üniversitesi Veteriner Bilimleri Dergisi     Open Access  
Australian Equine Veterinarian     Full-text available via subscription   (Followers: 2)
Australian Veterinary Journal     Hybrid Journal   (Followers: 10)
Avances en Ciencias Veterinarias     Open Access  
Avian Diseases     Full-text available via subscription   (Followers: 5)
Avian Diseases Digest     Full-text available via subscription   (Followers: 3)
Avian Pathology     Hybrid Journal   (Followers: 3)
Bangladesh Journal of Animal Science     Open Access  
Bangladesh Journal of Veterinary Medicine     Open Access   (Followers: 1)
Bangladesh Veterinarian     Open Access   (Followers: 1)
BMC Veterinary Research     Open Access   (Followers: 5)
Brazilian Journal of Veterinary Research and Animal Science     Open Access   (Followers: 7)
Buletin Peternakan : Bulletin of Animal Science     Full-text available via subscription  
Bulletin of Animal Health and Production in Africa     Full-text available via subscription   (Followers: 1)
Bulletin of the Veterinary Institute in Pulawy     Open Access  
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access  
Canadian Journal of Veterinary Research     Full-text available via subscription   (Followers: 7)
Case Reports in Veterinary Medicine     Open Access   (Followers: 4)
Ciência Animal Brasileira     Open Access  
Ciência Rural     Open Access   (Followers: 2)
Cogent Food & Agriculture     Open Access  
Companion Animal     Full-text available via subscription   (Followers: 5)
Domestic Animal Endocrinology     Hybrid Journal   (Followers: 2)
Equine Health     Full-text available via subscription   (Followers: 1)
Equine Veterinary Education     Hybrid Journal   (Followers: 8)
Equine Veterinary Journal     Hybrid Journal   (Followers: 11)
Ethiopian Veterinary Journal     Open Access   (Followers: 4)
Eurasian Journal of Veterinary Sciences     Open Access   (Followers: 1)
Frontiers in Veterinary Science     Open Access  
Global Journal of Animal Scientific Research     Open Access   (Followers: 1)
Human & Veterinary Medicine - International Journal of the Bioflux Society     Open Access   (Followers: 6)
ILAR Journal     Hybrid Journal  
In Practice     Full-text available via subscription   (Followers: 3)
Indian Journal of Animal Sciences     Open Access   (Followers: 5)
Indian Journal of Veterinary Anatomy     Full-text available via subscription   (Followers: 2)
Intas Polivet     Full-text available via subscription   (Followers: 1)
International Journal for Agro Veterinary and Medical Sciences     Open Access   (Followers: 3)
International Journal of Livestock Research     Open Access  
International Journal of Veterinary Science and Medicine     Open Access   (Followers: 4)
InVet     Open Access  
Iranian Journal of Applied Animal Science     Open Access  
Irish Veterinary Journal     Open Access   (Followers: 2)
Journal of Veterinary Science & Technology     Open Access   (Followers: 3)
Journal of Advanced Veterinary and Animal Research     Open Access   (Followers: 7)
Journal of Animal Behaviour and Biometeorology     Open Access   (Followers: 2)
Journal of Animal Physiology and Animal Nutrition     Hybrid Journal   (Followers: 5)
Journal of Animal Science and Technology     Open Access  
Journal of Applied Animal Nutrition     Hybrid Journal   (Followers: 3)
Journal of Avian Medicine and Surgery     Full-text available via subscription   (Followers: 5)
Journal of Buffalo Science     Hybrid Journal  
Journal of Equine Veterinary Science     Hybrid Journal   (Followers: 10)
Journal of Exotic Pet Medicine     Full-text available via subscription   (Followers: 4)
Journal of Experimental and Applied Animal Sciences     Open Access  
Journal of Feline Medicine & Surgery     Hybrid Journal   (Followers: 5)
Journal of Research in Forestry, Wildlife and Environment     Open Access   (Followers: 3)
Journal of Small Animal Practice     Hybrid Journal   (Followers: 10)
Journal of the American Veterinary Medical Association     Full-text available via subscription   (Followers: 25)
Journal of the Selva Andina Research Society     Open Access  
Journal of the South African Veterinary Association     Open Access   (Followers: 1)
Journal of Venomous Animals and Toxins     Open Access   (Followers: 4)
Journal of Veterinary Advances     Open Access   (Followers: 3)
Journal of Veterinary Behavior: Clinical Applications and Research     Hybrid Journal   (Followers: 3)
Journal of Veterinary Cardiology     Full-text available via subscription   (Followers: 4)
Journal of Veterinary Diagnostic Investigation     Hybrid Journal   (Followers: 7)
Journal of Veterinary Emergency and Critical Care     Hybrid Journal   (Followers: 13)
Journal of Veterinary Internal Medicine     Open Access   (Followers: 15)
Journal of Veterinary Medical Education     Partially Free   (Followers: 11)
Journal of Veterinary Medicine     Open Access   (Followers: 4)
Journal of Veterinary Medicine and Animal Health     Open Access   (Followers: 2)
Journal of Veterinary Pharmacology and Therapeutics     Hybrid Journal   (Followers: 5)

        1 2 3     

Journal Cover   Journal of Veterinary Diagnostic Investigation
  [SJR: 0.677]   [H-I: 53]   [7 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1040-6387 - ISSN (Online) 1943-4936
   Published by Sage Publications Homepage  [821 journals]
  • Characterization of Mannheimia haemolytica in beef calves via
           nasopharyngeal culture and pulsed-field gel electrophoresis
    • Authors: Capik, S. F; White, B. J, Lubbers, B. V, Apley, M. D, Mosier, D. A, Larson, R. L, Murray, R. W.
      Pages: 568 - 575
      Abstract: Mannheimia haemolytica is a major bacterial component of bovine respiratory disease (BRD); unfortunately, very little is known about M. haemolytica transmission dynamics among cattle. Identifying potential variation in M. haemolytica populations over time and induction of nasopharyngeal colonization and subsequent shedding are 2 areas where knowledge is lacking. In our study, 2 separate loads of 20 mixed-origin, male calves were purchased through an order buyer on different dates. Deep nasopharyngeal cultures (NPC) were performed on all calves on arrival and, if M. haemolytica–negative, a second screening culture was obtained. Calves that were negative on 2 initial NPCs (NEG; n = 4) were subsequently challenged with a previously isolated field strain of M. haemolytica in both the upper and lower respiratory tract, individually housed, and then monitored for M. haemolytica shedding via NPCs at 0.5, 1, 3, 5, 7, and 9 days postchallenge. Naturally M. haemolytica–positive calves (2 per load) were kept for additional daily cultures (POS; n = 4). Individual calf M. haemolytica status for both the POS and NEG groups was inconsistent between study days. Additionally, pulsed-field gel electrophoresis performed on isolates from the positive cultures showed that the NEG calves did not shed the M. haemolytica challenge strain, but rather 2 distinct clusters of M. haemolytica were shared among POS and NEG calves regardless of their initial status. Although sample sizes were small, these findings illustrate how variable the results of a single nasopharyngeal swab can be and the challenges of using an individual culture to truly represent animal M. haemolytica status.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715597724
      Issue No: Vol. 27, No. 5 (2015)
  • Use of serologic tests to predict resistance to Canine distemper
           virus-induced disease in vaccinated dogs
    • Authors: Jensen, W. A; Totten, J. S, Lappin, M. R, Schultz, R. D.
      Pages: 576 - 580
      Abstract: The objective of the current study was to determine whether detection of Canine distemper virus (CDV)-specific serum antibodies correlates with resistance to challenge with virulent virus. Virus neutralization (VN) assay results were compared with resistance to viral challenge in 2 unvaccinated Beagle puppies, 9 unvaccinated Beagle dogs (4.4–7.2 years of age), and 9 vaccinated Beagle dogs (3.7–4.7 years of age). Eight of 9 (89%) unvaccinated adult dogs exhibited clinical signs after virus challenge, and 1 (13%) dog died. As compared to adult dogs, the 2 unvaccinated puppies developed more severe clinical signs and either died or were euthanized after challenge. In contrast, no clinical signs were detected after challenge of the 9 adult vaccinated dogs with post-vaccination intervals of up to 4.4 years. In vaccinated dogs, the positive and negative predictive values of VN assay results for resistance to challenge were 100% and 0%, respectively. Results indicate that dogs vaccinated with modified live CDV can be protected from challenge for ≤4.4 years postvaccination and that detection of virus-specific antibodies is predictive of whether dogs are resistant to challenge with virulent virus. Results also indicate that CDV infection in unvaccinated dogs results in age-dependent morbidity and mortality. Knowledge of age-dependent morbidity and mortality, duration of vaccine-induced immunity, and the positive and negative predictive values of detection of virus-specific serum antibodies are useful in development of rational booster vaccination intervals for the prevention of CDV-mediated disease in adult dogs.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715602291
      Issue No: Vol. 27, No. 5 (2015)
  • Multiple antigen target approach using the Accuplex4 BioCD system to
           detect Borrelia burgdorferi antibodies in experimentally infected and
           vaccinated dogs
    • Authors: Moroff, S; Woodruff, C, Woodring, T, Sokolchik, I, Lappin, M. R.
      Pages: 581 - 588
      Abstract: The primary objective of our study was to optimize detection of serum antibodies to Borrelia burgdorferi using a new commercial automated fluorescence system (Accuplex4 BioCD system, Antech Diagnostics, Lake Success, New York). The system used multiple natural and artificial peptides—outer surface proteins (OspA, OspC, OspF), an outer membrane protein (P39), and a proprietary synthetic peptide (small Lyme peptide [SLP])—and the results were compared with a commercially available enzyme-linked immunosorbent assay that uses a proprietary peptide (C6). Sera from 4 groups were evaluated: dogs vaccinated with 1 of 3 commercially available vaccines (n = 18); dogs infested with adult Ixodes scapularis (black-legged tick; n = 18); dogs previously vaccinated and then infested with I. scapularis (n = 18); and dogs with B. burgdorferi infection that were then vaccinated (n = 14). All of the vaccines evaluated induced OspA responses. However, antibodies against OspF or C6 were not induced in any of the vaccinated dogs. Additionally, the OspF antibodies had 100% sensitivity and specificity when compared to antibodies against C6 peptide. In B. burgdorferi–infected dogs, antibodies against OspC and SLP were detected in serum sooner than antibodies against the other targets. Low levels of antibodies against OspA developed in 6 of 14 B. burgdorferi–infected, unvaccinated dogs and had the shortest duration compared to the other antibodies. Detection of antibody responses to multiple B. burgdorferi targets with this system can be used to help differentiate vaccinated dogs from exposed dogs as well as acute infection from chronic infection.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715600196
      Issue No: Vol. 27, No. 5 (2015)
  • Cutaneous histiocytic sarcoma with E-cadherin expression in a Pembroke
           Welsh Corgi dog
    • Authors: Hirako, A; Sugiyama, A, Sakurai, M, Ozaki, K, Sakai, H, Takeuchi, T, Morita, T, Moore, P. F.
      Pages: 589 - 595
      Abstract: An 11-year-old male neutered Pembroke Welsh Corgi dog displayed a mass measuring 7.5 cm x 6.6 cm x 1.6 cm in the skin. Neoplastic tissue was nonencapsulated, and the neoplastic cells showed infiltrative growth into the surrounding tissue on microscopic examination. The neoplastic tissue was mainly located from the dermis to the subcutis. Epidermotropism of neoplastic cells was not observed. The tissue was composed of irregular, solid nests of round to polygonal cells. Nests were separated by fine fibrovascular stroma. Mitotic index was high (7.90 ± 0.38 per high power field) and extensive necrosis was observed in the neoplastic tissue. Vascular invasion was often observed in the neoplastic tissue. Neoplastic cells were positive for vimentin, HLA-DR antigen, Iba1, CD18, and E-cadherin, but cells did not express cytokeratin, S100, CD20, CD79α, CD3, MUM-1, lambda light chain, kappa light chain, lysozyme, CD204, or CD11d by immunohistochemistry. Electron microscopic analysis revealed dendrites on these cells. From the above-mentioned findings, the tumor was diagnosed as a cutaneous histiocytic sarcoma with E-cadherin expression. It is possible that neoplastic cells in the present case were derived from cutaneous Langerhans cell. To our knowledge, cutaneous histiocytic sarcoma with E-cadherin expression in domestic animals has not been previously diagnosed in domestic animals.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715604185
      Issue No: Vol. 27, No. 5 (2015)
  • Screening for JH1 genetic defect carriers in Jersey cattle by a polymerase
           chain reaction and restriction fragment length polymorphism assay
    • Authors: Zhang, Y; Guo, G, Huang, H, Lu, L, Wang, L, Fang, L, Liu, L, Wang, Y, Zhang, S.
      Pages: 596 - 599
      Abstract: An autosomal recessive genetic defect termed JH1 has been associated with early embryonic loss in the Jersey cattle breed. The genetic basis has been identified as a cytosine to thymine mutation in the CWC15 gene that changes an amino acid from arginine to a stop code. To screen for JH1 carriers in an imported Jersey population in China, a method based on a polymerase chain reaction amplification followed by a restriction fragment length polymorphism assay (PCR-RFLP) was developed for the accurate diagnosis of the JH1 allele. A total of 449 randomly chosen cows were examined with the PCR-RFLP assay, and 31 were identified as JH1 carriers, corresponding to a carrier frequency of 6.9%. The PCR-RFLP method was validated by DNA sequencing of 8 positive and 13 negative samples, with all 21 samples giving the expected DNA sequence. In addition, 3 negative and 3 positive samples were confirmed by a commercial microarray-based single nucleotide polymorphism assay. Finally, samples from 9 bulls in the United States of known status were correctly identified as carriers (5 bulls) or noncarriers (4 bulls). As the JH1 defect has most likely spread worldwide, implementing routine screening is necessary to avoid the risk of carrier-to-carrier matings and to gradually eradicate the deleterious gene.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715589362
      Issue No: Vol. 27, No. 5 (2015)
  • Outbreak of variant pseudorabies virus in Bartha-K61-vaccinated piglets in
           central Shandong Province, China
    • Authors: Hu, D; Zhang, Z, Lv, L, Xiao, Y, Qu, Y, Ma, H, Niu, Y, Wang, G, Liu, S.
      Pages: 600 - 605
      Abstract: An epidemic that mainly endangered 3–7-day-old piglets struck many farms in Shandong Province, China in 2013 and caused heavy losses. To identify the pathogenesis, the type of lesions, and the causative agent, systemic examinations were performed. Autopsy showed multiple lesions, including necrotic foci of the spleen and liver, punctate hemorrhage of the renal cortex, and interstitial pneumonia. Histological examinations showed typical nonsuppurative encephalitis, necrotic lymphocytes, and reticuloendothelial cells in lymphatic tissues, as well as eosinophilic inclusion bodies in the nuclei of reticuloendothelial cells, necrotic foci in liver cells, and hemorrhagic glomeruli. The average seroprevalence rate of field pseudorabies virus (PRV; Suid herpesvirus 1) of a representative farm tested by enzyme-linked immunosorbent assay was 46%, indicating that the PRV infectious pressure was quite severe especially among gilts, young multiparous sows, boars, and growing–finishing pigs. The glycoprotein E (gE) gene of PRV was detected in 8 of 10 clinical samples, and the virus in the positive samples induced obvious cytopathic effects. An immunoperoxidase monolayer assay showed that PRV antigens were distributed both in the nucleoli and cytoplasm of infected cells. Sequencing and phylogenetic analysis of the gE gene showed that the strain isolated herein, TaiAn SD 2013, was highly similar to previously isolated strains, especially those isolated in northern China in 2013, and was closely related to other isolates from Asia. Evidence confirmed that the variant PRV was the etiologic agent of this epidemic, suggesting that the Bartha-K61 vaccine does not provide complete protection against PRV infection. Further challenge tests are ongoing to investigate the virulence of variant PRV.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715593599
      Issue No: Vol. 27, No. 5 (2015)
  • Assessment of litter prevalence of Mycoplasma hyopneumoniae in preweaned
           piglets utilizing an antemortem tracheobronchial mucus collection
           technique and a real-time polymerase chain reaction assay
    • Authors: Vangroenweghe, F; Karriker, L, Main, R, Christianson, E, Marsteller, T, Hammen, K, Bates, J, Thomas, P, Ellingson, J, Harmon, K, Abate, S, Crawford, K.
      Pages: 606 - 610
      Abstract: The swine industry currently lacks validated antemortem methods of detecting baseline herd prevalence of Mycoplasma hyopneumoniae. The focus of our study was to evaluate alternative antemortem detection techniques and to determine baseline litter prevalence in preweaned pig populations utilizing the selected technique and a real-time polymerase chain reaction (qPCR) assay. Preliminary data was analyzed on weaned piglets with evidence of respiratory disease (n = 32). Five sample types (antemortem nasal swab, tracheobronchial mucus, postmortem deep airway swab, bronchoalveolar lavage, and lung tissue) were collected from each pig. Individual samples were tested for M. hyopneumoniae using qPCR. Compared to nasal swabs, tracheobronchial mucus demonstrated higher test sensitivity (P < 0.0001). Tracheobronchial mucus was collected from apparently healthy preweaned piglets (n = 1,759; sow farms 1–3) and preweaned piglets exhibiting signs of respiratory disease (n = 32; sow farm 4), ranging in age from 12 to 25 days. Samples from sow farms 1–3 were pooled into 2 groups of 5 per litter (n = 360 pools from 180 litters), and qPCR was utilized to analyze each pool. A qPCR-positive result, threshold cycle
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715595062
      Issue No: Vol. 27, No. 5 (2015)
  • Giant axonal neuropathy-like disease in an Alexandrine parrot (Psittacula
    • Authors: Stent, A; Gosbell, M, Tatarczuch, L, Summers, B. A.
      Pages: 611 - 615
      Abstract: A chronic progressive neurological condition in an Alexandrine parrot (Psittacula eupatria) was manifest as intention tremors, incoordination, and seizure activity. Histology revealed large eosinophilic bodies throughout the central nervous system, and electron microscopy demonstrated that these bodies were greatly expanded axons distended by short filamentous structures that aggregated to form long strands. The presence of periodic acid–Schiff-positive material within the neuronal bodies of Purkinje cells and ganglionic neurons is another distinctive feature of this disease. The histological features of this case display some features consistent with giant axonal neuropathy as reported in humans and dogs. Based on investigation of the lineage in this case, an underlying inherited defect is suspected, but some additional factor appears to have altered the specific disease presentation in this bird.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715603423
      Issue No: Vol. 27, No. 5 (2015)
  • A comparative study of Toxoplasma gondii seroprevalence in mink using a
           modified agglutination test, a Western blot, and enzyme-linked
           immunosorbent assays
    • Authors: Gu, Y; Wang, Z, Cai, Y, Li, X, Wei, F, Shang, L, Li, J, Liu, Q.
      Pages: 616 - 620
      Abstract: Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P > 0.05), but a significant difference was observed between MAT and WB (P < 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB ( = 0.83), and a substantial agreement (92.4–93.1%) was observed in the TSA- and GRA1-ELISAs ( = 0.68–0.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74–0.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715596033
      Issue No: Vol. 27, No. 5 (2015)
  • Dermatophytosis in farmed mink (Mustela vison) caused by Trichophyton
    • Authors: Overy, D. P; Marron-Lopez, F, Muckle, A, Bourque, A, Lund, L, MacHattie, D, Lopez, A.
      Pages: 621 - 626
      Abstract: This report details 2 outbreaks of dermatophytosis in 2 different mink ranches. On the first farm, only kits were affected, while on the second farm, small numbers of adults were infected. Affected mink were otherwise clinically healthy and in good body condition. Three animals were euthanized and submitted for autopsy. Grossly, mink exhibited locally extensive to coalescing areas of crusting alopecia but no other significant gross lesions in internal organs. Microscopically, skin lesions were characterized by chronic hyperplastic dermatitis with folliculitis, furunculosis, occasional intracorneal pustules, and large numbers of intrafollicular fungal arthrospores and hyphae. The dermatophyte was cultured and identified as Trichophyton equinum based on molecular barcoding of the internal transcribed spacer region of the ribosomal DNA gene.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715596036
      Issue No: Vol. 27, No. 5 (2015)
  • Canine dysautonomia in a litter of Havanese puppies
    • Authors: Hull, N. C; O'Toole, D, Miller, M. M, Shoults, H, Deck, R, Jones, W, Johnson, G. C, Shaw, D. P, Schumaker, B. A.
      Pages: 627 - 631
      Abstract: Canine dysautonomia is a sporadic, generally fatal disease that rarely affects groups of related animals. Four 10-week-old Havanese puppies from a litter of 5 developed clinical signs of canine dysautonomia. The 4 affected dogs were exposed to an outdoor environment, whereas the fifth littermate was not exposed to the outdoors and remained clinically healthy. Clinical signs of dysautonomia developed 10–16 days after going outside the house. An unrelated dog also developed dysautonomia after exposure to 1 of the affected Havanese littermates. All 5 dogs had morphological changes consistent with dysautonomia (widespread neuronal degeneration in autonomic ganglia, select brainstem nuclei, and ventral horn motor neurons). Differential diagnoses were excluded through negative toxicological evaluation, fecal parasite screening, negative Canine distemper virus reverse transcription polymerase chain reaction, fluorescent antibody testing, attempted virus isolation, and electron microscopy. The 5 affected dogs were in the Kansas City, Missouri area, where there is a high incidence of dysautonomia.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715595838
      Issue No: Vol. 27, No. 5 (2015)
  • Acute death associated with Citrobacter freundii infection in an African
           elephant (Loxodonta africana)
    • Authors: Ortega, J; Corpa, J. M, Orden, J. A, Blanco, J, Carbonell, M. D, Gerique, A. C, Latimer, E, Hayward, G. S, Roemmelt, A, Kraemer, T, Romey, A, Kassimi, L. B, Casares, M.
      Pages: 632 - 636
      Abstract: A 21-year-old male African elephant (Loxodonta africana) died suddenly with no previous medical history. Grossly, there were severe multifocal epicardial and endocardial hemorrhages of the atria and ventricles, hydropericardium, multifocal pleural hemorrhages, and severe pulmonary congestion and edema. Histologically, there was fibrinoid vasculitis and thrombosis in the heart and lung and myocardial necrosis. Citrobacter freundii was isolated in abundance in pure culture from liver and heart samples. Low levels of multiples types of elephant endotheliotropic herpesvirus (EEHV-6, EEHV-2B, and EEHV-3A) were detected in spleen samples, but not in heart samples. The levels of EEHV DNA found were much lower than those usually associated with acute EEHV hemorrhagic disease, and many other genomic loci that would normally be found in such cases were evidently below the level of detection. Therefore, these findings are unlikely to indicate lethal EEHV disease. Polymerase chain reaction for encephalomyocarditis virus (EMCV) and toxicology for oleander (Nerium oleander) were negative. Stress, resulting from recent transport, and antimicrobial therapy may have contributed to the death of this animal.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715596034
      Issue No: Vol. 27, No. 5 (2015)
  • Glomerulonephritis in a ferret with feline coronavirus infection
    • Authors: Fujii, Y; Tochitani, T, Kouchi, M, Matsumoto, I, Yamada, T, Funabashi, H.
      Pages: 637 - 640
      Abstract: A male domestic ferret (Mustela putorius furo), which was purchased from outside of Japan at 13 weeks of age, was euthanized at 18 months of age because of poor health. At autopsy, the liver, spleen, and mesenteric lymph node were enlarged, and white foci were observed on the outer surface of the liver. The outer surface of the mesenteric lymph node was dark red. Histologically, granulomas were observed in the liver, spleen, bone marrow, and lymph nodes, composed mainly of aggregated epithelioid macrophages, some of which were positive to an anti–feline coronavirus (FCoV; Alphacoronavirus 1) antibody in immunohistochemistry. Mesangioproliferative glomerulonephritis was observed, and periodic acid–Schiff-positive deposits were observed along glomerular capillary walls. These deposits stained pale red with periodic acid–methenamine silver stain and red with Masson trichrome stain, and were also observed in the mesangial matrix. In affected glomeruli, glomerular capillary walls and mesangial areas were positive for anti-ferret immunoglobulin G. By electron microscopy, subepithelial and mesangial electron-dense deposits were observed consistent with immune complex deposition. The deposition of immune complexes may have been associated with FCoV infection.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715599570
      Issue No: Vol. 27, No. 5 (2015)
  • Yersiniosis due to infection by Yersinia pseudotuberculosis 4b in captive
           meerkats (Suricata suricatta) in Japan
    • Authors: Nakamura, S.-i; Hayashidani, H, Yonezawa, A, Suzuki, I, Une, Y.
      Pages: 641 - 644
      Abstract: Two meerkats (Suricata suricatta) housed in the same zoological garden in Japan died due to Yersinia pseudotuberculosis serotype 4b infection. Gross and microscopic lesions included necrotizing enteritis and enlargement of the spleen and liver with multifocal necrosis. Inflammatory cells, primarily neutrophils, and nuclear debris were associated with clusters of Gram-negative bacilli. Additionally, there were aberrant organism forms that were larger than bacilli and appeared as basophilic globular bodies. Immunohistochemical examination showed that the bacilli and globular bodies were strongly positive for Y. pseudotuberculosis O4 antigen. The globular bodies were considered a shape-changed form of Y. pseudotuberculosis, and these morphologically abnormal bacteria could present a diagnostic challenge.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715596035
      Issue No: Vol. 27, No. 5 (2015)
  • Comparison of primer sets for T-cell clonality testing in canine
           intestinal lymphoma
    • Authors: Takanosu, M; Kagawa, Y.
      Pages: 645 - 650
      Abstract: Clonality testing based on polymerase chain reaction is an important tool for diagnosis of lymphoproliferative diseases. Many primers have been designed and used for canine clonality testing. Canine intestinal lymphoma is usually diagnosed pathologically by examination of excised intestinal or endoscopic biopsy tissues, and clonality testing is sometimes used to support the pathological diagnosis if this examination is inconclusive. In the present study, the sensitivity of each previously published primer set for clonality testing was examined by using formalin-fixed, paraffin-embedded sections from 39 cases pathologically diagnosed as canine intestinal lymphoma (large-cell type). All 39 cases were immunohistochemically positive for cluster of differentiation (CD)3. Thirty-two out of the 39 cases showed clonality in the T-cell receptor gamma (TRG) with at least 1 of the tested primers. The primer set with the highest sensitivity detected all 32 cases with TRG clonality, with a sensitivity of 82.1%. These results provide useful evidence for the selection of primer sets for clonality testing of canine intestinal lymphoma.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715600197
      Issue No: Vol. 27, No. 5 (2015)
  • Bovine tuberculosis in an Asian small-clawed otter (Aonyx cinerea) in the
           Republic of Korea
    • Authors: Lee, H; Kim, J.-M, Jang, Y, Lee, K, Baek, K, Lee, B, Kim, H.-Y, Lee, M.-H, Ryoo, S, Bae, Y.-C, Choi, E.-J, So, B.
      Pages: 651 - 655
      Abstract: Bovine tuberculosis caused by Mycobacterium bovis has a wide range of hosts including cattle and humans, but its incidence in otters is very rare. Our report describes a case of bovine tuberculosis in an Asian small-clawed otter (Aonyx cinerea). A deceased female otter ~2–3 years of age that was raised in an aquarium was submitted to the Animal and Plant Quarantine Agency (Anyang, Republic of Korea) for autopsy in June 2013. Following gross pathological examination, many white nodules were observed in the lungs and mesentery. The nodules showed central necrosis infiltrated with lymphocytes and macrophages and surrounded by fibrous tissue. Acid-fast bacteria were detected in the necrotic foci, but no fungi were observed. Molecular analysis led to the detection of M. bovis, which is identified in otters in some European countries such as Spain and France.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715600198
      Issue No: Vol. 27, No. 5 (2015)
  • Cervical chordoma in a domestic ferret (Mustela putorius furo) with
           pulmonary metastasis
    • Authors: Frohlich, J. R; Donovan, T. A.
      Pages: 656 - 659
      Abstract: A 4-year-old, male neutered domestic ferret (Mustela putorius furo) was evaluated for a mass in the left cervical region. The owner elected humane euthanasia, and an autopsy was performed, revealing a neoplasm with infiltration into the left cranial articular fovea of the atlas and cervical vertebrae, with regional compression of the spinal cord. Histologic evaluation was consistent with cervical chordoma. At autopsy, a left cranial lung lobe nodule was observed. Additional sectioning and histologic evaluation revealed multiple foci of metastatic chordoma at this site. A small focus of micrometastasis was also detected in a section from the right lung lobes. Chordoma is the most common musculoskeletal neoplasm of ferrets, arising from remnant fetal notochord. To our knowledge, pulmonary chordoma metastasis has not been previously reported in the ferret. This case demonstrates the potential for visceral metastasis of chordoma in the ferret, as has been reported in other species.
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715603422
      Issue No: Vol. 27, No. 5 (2015)
  • Book Review: Arresting Contagion: Science, Policy, and Conflicts Over
           Animal Disease Control
    • Authors: O'Toole; D.
      Pages: 660 - 660
      PubDate: 2015-09-24T13:05:25-07:00
      DOI: 10.1177/1040638715606675
      Issue No: Vol. 27, No. 5 (2015)
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