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  Subjects -> NUTRITION AND DIETETICS (Total: 215 journals)
Showing 1 - 64 of 64 Journals sorted alphabetically
Acta Portuguesa de Nutrição     Open Access   (Followers: 1)
Advances in Digestive Medicine     Open Access   (Followers: 15)
Advances in Eating Disorders : Theory, Research and Practice     Hybrid Journal   (Followers: 20)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 73)
Advances in Nutrition     Hybrid Journal   (Followers: 64)
African Journal of Biomedical Research     Open Access   (Followers: 1)
African Journal of Food, Agriculture, Nutrition and Development     Open Access   (Followers: 27)
Aktuelle Ernährungsmedizin     Hybrid Journal   (Followers: 6)
Alimentos e Nutrição     Open Access   (Followers: 2)
American Journal of Botany     Full-text available via subscription   (Followers: 17)
American Journal of Clinical Nutrition     Hybrid Journal   (Followers: 239)
American Journal of Food and Nutrition     Open Access   (Followers: 60)
American Journal of Food Technology     Open Access   (Followers: 11)
Amerta Nutrition     Open Access  
Amino Acids     Hybrid Journal   (Followers: 8)
Annals of Nutrition and Metabolism     Full-text available via subscription   (Followers: 65)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 15)
Annual Review of Nutrition     Full-text available via subscription   (Followers: 43)
Appetite     Hybrid Journal   (Followers: 25)
Arab Journal of Nutrition and Exercise     Open Access   (Followers: 1)
Arbor Clinical Nutrition Updates     Full-text available via subscription   (Followers: 5)
Archive of Food and Nutritional Science     Open Access   (Followers: 2)
Archivos Latinoamericanos de Nutrición     Open Access   (Followers: 2)
Asia Pacific Journal of Clinical Nutrition     Full-text available via subscription   (Followers: 13)
Asian Journal of Clinical Nutrition     Open Access   (Followers: 14)
Australian Coeliac     Full-text available via subscription   (Followers: 2)
Bangladesh Journal of Nutrition     Open Access   (Followers: 7)
Better Nutrition     Full-text available via subscription   (Followers: 3)
Bioactive Carbohydrates and Dietary Fibre     Hybrid Journal   (Followers: 3)
Biomedicine & Preventive Nutrition     Partially Free   (Followers: 8)
BMC Nutrition     Open Access   (Followers: 11)
BMJ Nutrition, Prevention & Health     Open Access   (Followers: 5)
British Journal Of Nutrition     Hybrid Journal   (Followers: 102)
Cahiers de Nutrition et de Diététique     Full-text available via subscription   (Followers: 5)
Canadian Food Studies / La Revue canadienne des études sur l'alimentation     Open Access   (Followers: 1)
Canadian Journal of Dietetic Practice and Research     Full-text available via subscription   (Followers: 14)
Case Reports in Clinical Nutrition     Open Access   (Followers: 1)
Childhood Obesity     Hybrid Journal   (Followers: 24)
Clinical Nutrition     Hybrid Journal   (Followers: 96)
Clinical Nutrition ESPEN     Hybrid Journal   (Followers: 21)
Clinical Nutrition Experimental     Open Access   (Followers: 1)
Clinical Nutrition Insight     Full-text available via subscription   (Followers: 14)
Clinical Nutrition Supplements     Hybrid Journal   (Followers: 16)
Clinical Obesity     Hybrid Journal   (Followers: 19)
Comparative Exercise Physiology     Hybrid Journal   (Followers: 25)
Current Developments in Nutrition     Open Access   (Followers: 5)
Current Nutrition & Food Science     Hybrid Journal   (Followers: 28)
Current Nutrition Reports     Hybrid Journal   (Followers: 9)
Current Opinion in Clinical Nutrition & Metabolic Care     Hybrid Journal   (Followers: 23)
Current Research in Nutrition and Food Science     Open Access   (Followers: 6)
DEMETRA : Alimentação, Nutrição & Saúde     Open Access  
Diabetes, Metabolic Syndrome and Obesity     Open Access   (Followers: 50)
Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity     Hybrid Journal   (Followers: 24)
Ecology of Food and Nutrition     Hybrid Journal   (Followers: 9)
Egyptian Journal of Obesity, Diabetes and Endocrinology     Open Access   (Followers: 1)
Endocrinología y Nutrición     Full-text available via subscription   (Followers: 2)
Endocrinología y Nutrición (English Edition)     Full-text available via subscription   (Followers: 2)
Ernährung & Medizin     Hybrid Journal   (Followers: 4)
European Journal of Clinical Nutrition     Hybrid Journal   (Followers: 82)
European Journal of Nutrition     Hybrid Journal   (Followers: 41)
European Journal of Nutrition & Food Safety     Open Access   (Followers: 3)
Flavour     Open Access   (Followers: 3)
Food & Nutrition Research     Open Access   (Followers: 36)
Food and Environmental Virology     Hybrid Journal   (Followers: 1)
Food and Foodways: Explorations in the History and Culture of     Hybrid Journal   (Followers: 10)
Food and Health     Open Access   (Followers: 1)
Food and Nutrition Bulletin     Hybrid Journal   (Followers: 6)
Food and Waterborne Parasitology     Open Access  
Food Digestion     Hybrid Journal   (Followers: 5)
Food Quality and Safety     Open Access   (Followers: 1)
Food Science & Nutrition     Open Access   (Followers: 61)
Food, Culture and Society: An International Journal of Multidisciplinary Research     Full-text available via subscription   (Followers: 11)
Frontiers in Nutrition     Open Access   (Followers: 11)
Frontiers in Sustainable Food Systems     Open Access   (Followers: 1)
Functional Foods in Health and Disease     Open Access  
Gazi Sağlık Bilimleri Dergisi     Open Access  
Genes & Nutrition     Open Access   (Followers: 9)
Hacettepe University Faculty of Health Sciences Journal     Open Access  
Harran Tarım ve Gıda Bilimleri Dergisi     Open Access   (Followers: 1)
Indian Journal of Nutrition and Dietetics     Hybrid Journal   (Followers: 1)
Indonesian Food and Nutrition Progress     Open Access  
International Journal for Vitamin and Nutrition Research     Hybrid Journal   (Followers: 12)
International Journal of Behavioral Nutrition and Physical Activity     Open Access   (Followers: 30)
International Journal of Child Health and Nutrition     Hybrid Journal   (Followers: 7)
International Journal of Eating Disorders     Hybrid Journal   (Followers: 31)
International Journal of Food Safety, Nutrition and Public Health     Hybrid Journal   (Followers: 23)
International Journal of Food Science and Nutrition Engineering     Open Access   (Followers: 10)
International Journal of Food Sciences and Nutrition     Hybrid Journal   (Followers: 12)
International Journal of Nutrition and Metabolism     Open Access   (Followers: 24)
International Journal of Nutrition, Pharmacology, Neurological Diseases     Open Access   (Followers: 4)
International Journal of Nutrology     Open Access  
International Journal of Obesity     Hybrid Journal   (Followers: 94)
International Journal of Sport Nutrition & Exercise Metabolism     Hybrid Journal   (Followers: 88)
Intrinsically Disordered Proteins     Hybrid Journal  
Journal of Acupuncture and Herbs     Open Access   (Followers: 3)
Journal of Agriculture, Food Systems, and Community Development     Open Access   (Followers: 1)
Journal of Dietary Supplements     Hybrid Journal   (Followers: 11)
Journal of Eating Disorders     Open Access   (Followers: 11)
Journal of Ethnic Foods     Open Access   (Followers: 2)
Journal of Food & Nutritional Disorders     Hybrid Journal   (Followers: 2)
Journal of Food and Nutrition Research     Open Access   (Followers: 11)
Journal of Food Chemistry and Nutrition     Open Access   (Followers: 6)
Journal of Food Science and Nutrition Therapy     Open Access   (Followers: 2)
Journal of Health, Population and Nutrition     Open Access   (Followers: 10)
Journal of Human Nutrition and Dietetics     Hybrid Journal   (Followers: 51)
Journal of Hunger & Environmental Nutrition     Hybrid Journal   (Followers: 6)
Journal of Medical Nutrition and Nutraceuticals     Open Access   (Followers: 3)
Journal of Medicinal Herbs and Ethnomedicine     Open Access  
Journal of Muscle Foods     Hybrid Journal  
Journal of Nutraceuticals and Herbal Medicine     Open Access   (Followers: 4)
Journal of Nutrition     Hybrid Journal   (Followers: 40)
Journal of Nutrition & Food Sciences     Open Access   (Followers: 6)
Journal of Nutrition & Intermediary Metabolism     Open Access  
Journal of Nutrition and Metabolism     Open Access   (Followers: 17)
Journal of Nutrition Education and Behavior     Hybrid Journal   (Followers: 21)
Journal of Nutrition in Gerontology and Geriatrics     Hybrid Journal   (Followers: 15)
Journal of Nutrition, Health and Aging     Hybrid Journal   (Followers: 31)
Journal of Nutritional & Environmental Medicine     Full-text available via subscription   (Followers: 2)
Journal of Nutritional Biochemistry     Hybrid Journal   (Followers: 8)
Journal of Nutritional Disorders & Therapy     Open Access  
Journal of Nutritional Ecology and Food Research     Full-text available via subscription  
Journal of Nutritional Science     Open Access   (Followers: 3)
Journal of Nutritional Therapeutics     Hybrid Journal   (Followers: 2)
Journal of Obesity     Open Access   (Followers: 27)
Journal of Parenteral and Enteral Nutrition     Hybrid Journal   (Followers: 33)
Journal of Pediatric Gastroenterology and Nutrition (JPGN)     Hybrid Journal   (Followers: 50)
Journal of Pharmacy and Nutrition Sciences     Open Access   (Followers: 8)
Journal of Probiotics & Health     Open Access   (Followers: 1)
Journal of Renal Nutrition     Hybrid Journal   (Followers: 28)
Journal of Renal Nutrition and Metabolism     Open Access   (Followers: 1)
Journal of Sensory Studies     Hybrid Journal   (Followers: 4)
Journal of Spices and Aromatic Crops     Open Access  
Journal of the Academy of Nutrition and Dietetics     Full-text available via subscription   (Followers: 60)
Journal of the American College of Nutrition     Hybrid Journal   (Followers: 8)
Journal of the American Dietetic Association     Full-text available via subscription   (Followers: 27)
Journal of the Australasian College of Nutritional and Environmental Medicine     Full-text available via subscription  
Jurnal Gizi dan Dietetik Indonesia : Indonesian Journal of Nutrition and Dietetics     Open Access   (Followers: 4)
Jurnal Gizi dan Pangan     Open Access   (Followers: 2)
Jurnal Gizi Indonesia / The Indonesian Journal of Nutrition     Open Access   (Followers: 3)
Jurnal Gizi Klinik Indonesia     Open Access   (Followers: 1)
Jurnal Penelitian Gizi dan Makanan     Open Access   (Followers: 4)
Jurnal Riset Kesehatan     Open Access  
La Ciencia al Servicio de la Salud y Nutrición     Open Access   (Followers: 1)
Lifestyle Genomics     Open Access   (Followers: 4)
Lifestyle Journal     Open Access   (Followers: 1)
Maternal & Child Nutrition     Hybrid Journal   (Followers: 13)
Médecine & Nutrition     Full-text available via subscription  
Media Gizi Indonesia     Open Access   (Followers: 1)
Metabolism and Nutrition in Oncology     Open Access   (Followers: 4)
Molecular Nutrition & Food Research     Hybrid Journal   (Followers: 8)
NFS Journal     Open Access  
Nigerian Food Journal     Full-text available via subscription   (Followers: 2)
Nigerian Journal of Nutritional Sciences     Full-text available via subscription  
Nutrafoods     Hybrid Journal  
Nutrición Hospitalaria     Open Access   (Followers: 4)
Nutridate     Full-text available via subscription   (Followers: 1)
Nutrients     Open Access   (Followers: 11)
Nutrire     Hybrid Journal   (Followers: 2)
Nutrition     Hybrid Journal   (Followers: 22)
Nutrition & Diabetes     Open Access   (Followers: 21)
Nutrition & Dietetics     Hybrid Journal   (Followers: 29)
Nutrition & Metabolism     Open Access   (Followers: 13)
Nutrition - Science en évolution     Full-text available via subscription   (Followers: 1)
Nutrition : X     Open Access   (Followers: 1)
Nutrition Action Health Letter     Free   (Followers: 3)
Nutrition and Cancer     Hybrid Journal   (Followers: 11)
Nutrition and Dietary Supplements     Open Access   (Followers: 14)
Nutrition and Health     Hybrid Journal   (Followers: 9)
Nutrition and Metabolic Insights     Open Access   (Followers: 4)
Nutrition Bulletin     Hybrid Journal   (Followers: 8)
Nutrition Bytes     Open Access   (Followers: 6)
Nutrition in Clinical Practice     Hybrid Journal   (Followers: 43)
Nutrition Journal     Open Access   (Followers: 8)
Nutrition Research     Hybrid Journal   (Followers: 21)
Nutrition Research Reviews     Hybrid Journal   (Followers: 13)
Nutrition Reviews     Hybrid Journal   (Followers: 33)
Nutrition Today     Hybrid Journal   (Followers: 12)
Nutrition, Metabolism and Cardiovascular Diseases     Hybrid Journal   (Followers: 12)
Nutritional Neuroscience : An International Journal on Nutrition, Diet and Nervous System     Hybrid Journal   (Followers: 8)
Obésité     Hybrid Journal   (Followers: 1)
Obesity     Hybrid Journal   (Followers: 58)
Obesity Facts     Open Access   (Followers: 9)
Obesity Reviews     Hybrid Journal   (Followers: 26)
Open Food Science Journal     Open Access  
Open Nutrition Journal     Open Access   (Followers: 1)
Open Obesity Journal     Open Access   (Followers: 1)
Pakistan Journal of Nutrition     Open Access   (Followers: 2)
Pediatric Obesity     Hybrid Journal   (Followers: 9)
Perspectivas en Nutrición Humana     Open Access   (Followers: 1)
PharmaNutrition     Hybrid Journal   (Followers: 1)
Plant Foods for Human Nutrition     Hybrid Journal   (Followers: 5)
Plant Production Science     Open Access  
Procedia Food Science     Open Access  
Proceedings of the Nutrition Society     Hybrid Journal   (Followers: 8)
Progress in Nutrition     Open Access   (Followers: 1)
Public Health Nutrition     Hybrid Journal   (Followers: 28)
RBNE - Revista Brasileira de Nutrição Esportiva     Open Access   (Followers: 1)
RBONE - Revista Brasileira de Obesidade, Nutrição e Emagrecimento     Open Access  
Recent Patents on Food, Nutrition & Agriculture     Hybrid Journal  
Revista Chilena de Nutricion     Open Access   (Followers: 2)

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Similar Journals
Journal Cover
Amino Acids
Journal Prestige (SJR): 1.135
Citation Impact (citeScore): 3
Number of Followers: 8  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1438-2199 - ISSN (Online) 0939-4451
Published by Springer-Verlag Homepage  [2626 journals]
  • Design of improved synthetic antifungal peptides with targeted variations
           in charge, hydrophobicity and chirality based on a correlation study
           between biological activity and primary structure of plant defensin
    • Abstract: Abstract Microbial resistance to available drugs is a growing health threat imposing the need for the development of new drugs. The scaffold of plant defensins, including their γ-cores, are particularly good candidates for drug design. This work aimed to improve the antifungal activity of a previous design peptide, named A36,42,44γ32–46VuDef (for short DD) against yeasts by altering its biochemical parameters. We explore the correlation of the biological activity and structure of plant defensins and compared their primary structures by superimposition with VuDef1 and DD which indicated us the favorable position and the amino acid to be changed. Three new peptides with modifications in charge, hydrophobicity (RR and WR) and chirality (D-RR) were designed and tested against pathogenic yeasts. Inhibition was determined by absorbance. Viability of mammalian cells was determined by MTT. The three designed peptides had better inhibitory activity against the yeasts with better potency and spectrum of yeast species inhibition, with low toxicity to mammalian cells. WR, the most hydrophobic and cationic, exhibited better antifungal activity and lower toxicity. Our study provides experimental evidence that targeted changes in the primary structure of peptides based on plant defensins γ-core primary structures prove to be a good tool for the synthesis of new compounds that may be useful as alternative antifungal drugs. The method described did not have the drawback of synthesis of several peptides, because alterations are guided. When compared to other methods, the design process described is efficient and viable to those with scarce resources.
      PubDate: 2021-01-23
  • IHEC_RAAC: a online platform for identifying human enzyme classes via
           reduced amino acid cluster strategy
    • Abstract: Abstract Enzymes have been proven to play considerable roles in disease diagnosis and biological functions. The feature extraction that truly reflects the intrinsic properties of protein is the most critical step for the automatic identification of enzymes. Although lots of feature extraction methods have been proposed, some challenges remain. In this study, we developed a predictor called IHEC_RAAC, which has the capability to identify whether a protein is a human enzyme and distinguish the function of the human enzyme. To improve the feature representation ability, protein sequences were encoded by a new feature-vector called ‘reduced amino acid cluster’. We calculated 673 amino acid reduction alphabets to determine the optimal feature representative scheme. The tenfold cross-validation test showed that the accuracy of IHEC_RAAC to identify human enzymes was 74.66% and further discriminate the human enzyme classes with an accuracy of 54.78%, which was 2.06% and 8.68% higher than the state-of-the-art predictors, respectively. Additionally, the results from the independent dataset indicated that IHEC_RAAC can effectively predict human enzymes and human enzyme classes to further provide guidance for protein research. A user-friendly web server, IHEC_RAAC, is freely accessible at http://bioinfor.imu.edu.cn/ihecraac.
      PubDate: 2021-01-23
  • Transglutaminase 2: a novel therapeutic target for idiopathic pulmonary
           fibrosis using selective small molecule inhibitors
    • Abstract: Abstract This study investigates the effects of a site-directed TG2-selective inhibitor on the lung myofibroblast phenotype and ECM deposition to elucidate TG2 as a novel therapeutic target in idiopathic pulmonary fibrosis (IPF)—an incurable progressive fibrotic disease. IPF fibroblasts showed increased expression of TG2, α smooth muscle actin (αSMA) and fibronectin (FN) with increased extracellular TG2 and transforming growth factor β1 (TGFβ1) compared to normal human lung fibroblasts (NHLFs) which do not express αSMA and express lower levels of FN. The myofibroblast phenotype shown by IPF fibroblasts could be reversed by selective TG2 inhibition with a reduction in matrix FN and TGFβ1 deposition. TG2 transduction or TGFβ1 treatment of NHLFs led to a comparable phenotype to that of IPF fibroblasts which was reversible following selective TG2 inhibition. Addition of exogenous TG2 to NHLFs also induced the myofibroblast phenotype by a mechanism involving TGFβ1 activation which could be ameliorated by selective TG2 inhibition. SMAD3-deleted IPF fibroblasts via CRISPR-cas9 genome editing, showed reduced TG2 protein levels following TGFβ1 stimulation. This study demonstrates a key role for TG2 in the induction of the myofibroblast phenotype and shows the potential for TG2-selective inhibitors as therapeutic agents for the treatment of fibrotic lung diseases like IPF.
      PubDate: 2021-01-21
  • Colorimetric recognition of aromatic amino acid enantiomers by gluconic
           acid-capped gold nanoparticles
    • Abstract: Abstract In this work, we prepared gold nanoparticles (AuNPs) by employing gluconic acid (GlcA) as reducing-cum-stabilizing agent. The proposed GlcA-AuNPs successfully worked as a colorimetric sensor for visual chiral recognition of aromatic amino acid enantiomers, namely tyrosine (d/l-Tyr), phenylalanine (d/l-Phe), and tryptophan (d/l-Trp). After adding L-types to GlcA-AuNPs solution, the color of the mixture changed from red to purple (or gray), while no obvious color change occurred on the addition of D-types. The effect can be detected by naked eyes. The particles have been characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, zeta potential, the dynamic light scattering analysis as well as UV–Vis spectroscopy. This assay can be used to determine the enantiomeric excess of l-Trp in the range from 0 to + 100%. The method has advantages in simplicity, sensitivity, fast response, and low cost.
      PubDate: 2021-01-12
  • Correction to: Dietary l -glutamine supplementation modulates microbial
           community and activates innate immunity in the mouse intestine
    • Abstract: In the published article, the images for Actin in Figs. 4a, 5a, 6a, and 6c were incorrectly presented.
      PubDate: 2021-01-11
  • Effect of ageing and hypertension on the expression and activity of PEPT2
           in normal and hypertrophic hearts
    • Abstract: Abstract Some dipeptides have been implicated in myocardial protection, but little is known about their membrane transporter PEPT2. The aim of this study was to determine whether the expression and activity of the cardiac-type PEPT2 cotransporter could be affected by ageing and/or hypertension. Sarcolemmal vesicles (SV) were isolated from the hearts of all rat groups using a standard procedure to investigate the transport activity and protein abundance by fluorescence spectroscopy and Western blot, respectively. SLC15A2 “PEPT2” gene expression was relatively quantified by RT-qPCR. In the Wistar rat groups, the protein and gene expression of PEPT2 were upregulated with ageing. These changes were accompanied by corresponding increases in the competitive inhibition and the transport rate (Vmax) of β-Ala-Lys (AMCA) into SV isolated from middle-aged hearts. Although, the transport rate of β-Ala-Lys (AMCA) into SV isolated from old hearts was significantly the lowest compared to middle-aged and young adult hearts, the inhibition percentage of β-Ala-Lys (AMCA) transport by Gly-Gln was the highest. In the WKY and SHR rat groups, Y-SHR hypertrophied hearts showed an increase in PEPT2 gene expression accompanied by a significant decrease in protein expression and activity. With advanced age, however, M-SHR hypertrophied hearts revealed significantly lower gene expression, but higher protein expression and activity than Y-SHR hearts. These findings suggest that increased expression of PEPT2 cotransporter in all types of middle-aged hearts could be exploited to facilitate di-and tripeptide transport by PEPT2 in these hearts, which subsequently could result in improved myocardial protection in these populations.
      PubDate: 2021-01-06
  • Arginine methylation: the promise of a ‘silver bullet’ for
           brain tumours'
    • Abstract: Abstract Despite intense research efforts, our pharmaceutical repertoire against high-grade brain tumours has not been able to increase patient survival for a decade and life expectancy remains at less than 16 months after diagnosis, on average. Inhibitors of protein arginine methyltransferases (PRMTs) have been developed and investigated over the past 15 years and have now entered oncology clinical trials, including for brain tumours. This review collates recent advances in the understanding of the role of PRMTs and arginine methylation in brain tumours. We provide an up-to-date literature review on the mechanisms for PRMT regulation. These include endogenous modulators such as alternative splicing, miRNA, post-translational modifications and PRMT–protein interactions, and synthetic inhibitors. We discuss the relevance of PRMTs in brain tumours with a particular focus on PRMT1, -2, -5 and -8. Finally, we include a future perspective where we discuss possible routes for further research on arginine methylation and on the use of PRMT inhibitors in the context of brain tumours.
      PubDate: 2021-01-06
  • Factors associated with elevated plasma phenylalanine in patients with
           heart failure
    • Abstract: Abstract Elevated phenylalanine has been observed in patients with advanced heart failure (HF) and in community cohorts at risk of HF, and has been shown to have prognostic value. This study aimed to explore the factors associated with elevated phenylalanine in HF patients. Mass spectrometry was performed on blood from 669 participants, including 75 normal controls and 594 HF patients (stages A, B, and C). We measured phenylalanine and associated degradation products on the catecholamine pathway, C-reactive protein, valerylcarnitine, methionine sulfoxide, estimated glomerular filtration rate (eGFR), and B-type natriuretic peptide. Longitudinal analysis was conducted on 61 stage C HF patients who had recovered systolic function after 1 year. Phenylalanine and tyrosine levels increased from normal through stages A, B and C. Cross-sectional analysis in patients at stage C showed that phenylalanine levels were related to total bilirubin, eGFR, valerylcarnitine, methionine sulfoxide, C-reactive protein, and male gender. Longitudinal analysis in the patients at stage C with recovered systolic function after 1 year revealed that phenylalanine, tyrosine, methionine sulfoxide, total bilirubin, and C-reactive protein levels significantly decreased from baseline to 12 months. Based on a generalized estimating equations analysis model with time interaction considered, the only significant factor associated with changes in phenylalanine was changes in C-reactive protein concentrations from baseline to 12 months [B (coefficient) = 0.81, P < 0.001] after adjusting for methionine sulfoxide and total bilirubin levels. In conclusion, phenylalanine levels respond sensitively to HF improvement. Our findings suggest that inflammation plays a pivotal role in the elevation of phenylalanine levels in patients with HF.
      PubDate: 2021-01-04
  • Chemical synthesis and characterisation of the complement C5 inhibitory
           peptide zilucoplan
    • Abstract: Abstract The complement component C5 inhibitory peptide zilucoplan is currently in phase III clinical trials for myasthenia gravis (MG). Despite being at an advanced stage of clinical development, there have been no published reports in the literature detailing its chemical synthesis. In this work, we describe an approach for the chemical synthesis of zilucoplan and validate that the synthesised compound blocks LPS-induced C5a production from human blood.
      PubDate: 2021-01-04
  • Plasma amino acids indicate glioblastoma with ATRX loss
    • Abstract: Abstract Glioblastoma (GB) is the most common primary brain tumour in adults. The lack of molecular biomarker, non-specific symptoms and fast growth rate often result in a significant delay in diagnosis. Despite multimodal treatment, the prognosis remains poor. Here, we verified the hypothesis that amino acids (AA) regulating the critical metabolic pathways necessary for maintenance, growth, reproduction, and immunity of an organism, may constitute a favourable target in GB biomarker research. We measured the plasma amino acids levels in 18 GB patients and 15 controls and performed the quantitative and qualitative metabolomic analysis of free AA applying high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). We present both the raw data and the results of our statistical analysis. The majority of AA were lowered in the study group in comparison to the control group. Five of these (arginine, glutamic acid, glutamine, glycine, and histidine) differed significantly (all p < 10–5 and AUC > 0.9). Plasma levels of leucine and phenylalanine decreased in the case of GB with lost alpha-thalassemia/mental retardation X-linked (ATRX) expression on immunohistochemistry (p = 0.003 and 0.045, respectively). We demonstrated for the first time that certain plasma-free AA levels of GB patients were significantly different from those in healthy volunteers. Target profiling of plasma-free AA, identified utilizing LC-QTOF-MS, may present prognostic value by indicating GB patients with lost ATRX expression. The on-going quest for glioma biomarkers still aims to determine the detailed metabolic profile and evaluate its impact on therapy and prognosis.
      PubDate: 2021-01-04
  • Effects of long-term taurine supplementation on age-related changes in
           skeletal muscle function of Sprague–Dawley rats
    • Abstract: Abstract Taurine (2-aminoethanesulfonic acid) is a free amino acid found abundantly in mammalian tissues. Increasing evidence suggests that taurine plays a role in the maintenance of skeletal muscle function and increase of exercise capacity. Most energy drinks contain this amino acid; however, there is insufficient research on the effects of long-term, low-dose supplementation of taurine. In this study, we investigated the effects of long-term administration of taurine at low doses on aging in rodents. In Experiment 1, we examined age-related changes in aging Sprague–Dawley (SD) rats (32–92 weeks old) that O2 consumption and spontaneous activity decreased significantly with aging. In Experiment 2, we examined the effects of long-term (21-week) administration of taurine on healthy aging SD rats. SD rats were stabilized for 32–34 weeks and divided into three groups, administrated water (control), 0.5% taurine (25 mg/kg  body weight (BW)/day), or 1% taurine (50 mg/kg  BW/day) from age 34 to 56 weeks (5 days/week, 5 mL/kg BW). Our findings suggest that long-term administration of taurine at relatively low dose could attenuate the age-related decline in O2 consumption and spontaneous locomotor activity. Upon intestinal absorption, taurine might modulate age-related changes in respiratory metabolism and skeletal muscle function via peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), succinate dehydrogenase (SDH), cytochrome c (Cycs), myocyte enhancer factor 2A (MEF2A), glucose transporter 4 (GLUT4), and myoglobin, which are regulated by the activation of AMP-activated protein kinase (AMPK). This article examines the mechanism underlying the effects of taurine on age-related changes, which may have potential clinical implications.
      PubDate: 2021-01-04
  • Use of alternative protein sources for fishmeal replacement in the diet of
           largemouth bass ( Micropterus salmoides ). Part II: effects of
           supplementation with methionine or taurine on growth, feed utilization,
           and health
    • Abstract: Abstract Fishmeal has long been a staple protein feedstuff for fish, but its global shortage and high price have prompted its replacement with alternative sustainable sources. In this experiment involving largemouth bass (a carnivorous fish), a new mixture of feedstuffs (45% poultry byproduct meal, 30% soybean meal, 15% blood meal, and 10% krill shrimp meal) was added to low (14.5%) fishmeal diets along with 0.0%, 0.5% taurine, 0.5% methionine, or 0.5% taurine plus 0.5% methionine (dry matter basis). The positive control diet [65.3% fishmeal (46% crude protein on dry matter basis)] and all low-fishmeal diets contained 40% true protein and 10% lipids. There were 3 tanks per treatment group (20 fish/tank). Fish with the mean initial body weight of 16.6 g were fed to satiety twice daily. Compared with the unsupplemented low-fishmeal group, supplementing either 0.5% methionine or 0.5% methionine plus 0.5% taurine to the low-fishmeal diet improved (P < 0.05) the growth, feed utilization, retention of dietary protein and lipids, and health of largemouth bass, reduced (P < 0.05) the occurrence of black skin syndrome from ~ 40 to ~ 10%. Histological sections of tissues from the fish with black skin syndrome showed retina degeneration, liver damage, and enteritis in the intestine. Compared with methionine supplementation, supplementing 0.5% taurine alone to the low-fishmeal diet did not affect the growth or feed efficiency of fish and had less beneficial effects (P < 0.05) on ameliorating the black skin syndrome. These results indicated that: (a) the basal low-fishmeal diet was inadequate in methionine or taurine; and (b) dietary supplementation with methionine was an effective method to improve the growth performance, feed efficiency, and health of largemouth bass. Further studies are warranted to understand the pathogenesis of the black skin syndrome in largemouth bass.
      PubDate: 2021-01-04
  • Reduction of oxidative stress and ornithine decarboxylase expression in a
           human prostate cancer cell line PC-3 by a combined treatment with
           α-tocopherol and naringenin
    • Abstract: Abstract Differentiation of a human aggressive PC-3 cancer cell line was obtained, in a previous investigation, by the synergic effect of α-tocopherol (α-TOC) and naringenin (NG). This combined treatment induced apoptosis and subsequent reduction of the PC-3 cell proliferation and invasion, by a pro-differentiating action. Since one of the peculiar characteristics of NG and α-TOC is their strong antioxidant activity, this study aimed to investigate their potential effect on the activity of the main enzymes involved in the antioxidant mechanism in prostate cancer cells. NG and α-TOC administered singularly or combined in the PC-3 cell line, affected the activity of several enzymes biomarkers of the cellular antioxidant activity, as well as the concentration of total glutathione (GSH + GSSG) and thiobarbituric acid reactive substances (TBARS). The combined treatment increased the TBARS levels and superoxide dismutase (SOD) activity, while decreased the glutathione S-transferase (GST), glutathione reductase (GR), and glyoxalase I (GI) activities. The results obtained indicate that a combined treatment with these natural compounds mitigated the oxidative stress in the human PC-3 cell line. In addition, a significant reduction of both ornithine decarboxylase (ODC) expression and intracellular levels of polyamines, both well-known positive regulators of cell proliferation, accompanied the reduction of oxidative stress observed in the combined α-TOC and NG treatment. Considering the established role of polyamines in cell differentiation, the synergism with NG makes α-TOC a potential drug for further study on the differentiation therapy in prostate cancer patients.
      PubDate: 2021-01-04
  • Angiotensin-(1–7) attenuates collagen-induced arthritis via inhibiting
           oxidative stress in rats
    • Abstract: Abstract The present study was designed to investigate the anti-rheumatic effects and the mechanism of angiotensin (Ang)-(1–7) in rat models with collagen-induced arthritis (CIA). The CIA model was established using male Wistar rats by intradermal injection of bovine collagen-II in complete Freund's adjuvant at the base of the tail. The levels of angiotensin converting enzyme 2 (ACE2)/Ang-(1–7)/Mas receptor (MasR) were reduced in CIA rats. The attenuation of paw swelling and arthritis scores and improvement of indexes of spleen and thymus were done by Ang-(1–7) injection in CIA rats. The increased levels of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in the serum and hind paw were blocked by Ang-(1–7) administration. In addition, enhanced NADPH oxidase (Nox) activity, increased levels of superoxide anions and malondialdehyde (MDA), and weakened superoxide dismutase (SOD) activity, were all reversed by treatment with Ang-(1–7). Nox1 overexpression reversed the suppressing effects of Ang-(1–7) on paw swelling and arthritis scores in CIA rats. The Ang-(1–7)-induced improvement in spleen and thymus indexes in CIA rats was abolished by Nox1 overexpression. Nox1 overexpression reversed the inhibitory effects of Ang-(1–7) by increasing IL-1β, IL-6, TNF-α, and IFN-γ levels in the serum and hind paw of CIA rats. These results demonstrated that Nox1 increased the oxidative stress in arthritis, and Ang-(1–7) improved rheumatism in arthritis via inhibiting oxidative stress.
      PubDate: 2021-01-04
  • The introduction of l -phenylalanine into antimicrobial peptide
           protonectin enhances the selective antibacterial activity of its
           derivative phe-Prt against Gram-positive bacteria
    • Abstract: Abstract Protonectin was a typical amphiphilic antimicrobial peptide with potent antimicrobial activity against Gram-positive and Gram-negative bacteria. In the present study, when its eleventh amino acid in the sequence was substituted by phenylalanine, the analog named phe-Prt showed potent antimicrobial activity against Gram-positive bacteria, but no antimicrobial activity against Gram-negative bacteria, indicating a significant selectivity between Gram-positive bacteria and Gram-negative bacteria. However, when Gram-negative bacteria were incubated with EDTA, the bacteria were susceptible to phe-Prt. Next, the binding effect of phe-Prt with LPS was determined. Our result showed that LPS could hamper the bactericidal activity of phe-Prt against Gram-positive bacteria. The result of zeta potential assay further confirmed the binding effect of phe-Prt with LPS for it could neutralize the surface charge of E. coli and LPS. Then, the effect of phe-Prt on the integrity of outer membrane of Gram-negative bacteria was determined. Our results showed that phe-Prt had a much weaker disturbance to the outer membrane of Gram-negative bacteria than the parent peptide protonectin. In summary, the introduction of l-phenylalanine into the sequence of antimicrobial peptide protonectin made phe-Prt show significant selectivity against Gram-positive bacteria, which could partly be attributed to the delay effect of LPS for phe-Prt to access to cell membrane. Although further study is still needed to clarify the exact mechanism of selectivity, the present study provided a strategy to develop antimicrobial peptides with selectivity toward Gram-positive and Gram-negative bacteria.
      PubDate: 2021-01-01
  • Isocyanide-based consecutive Bargellini/Ugi reactions: an efficient method
           for the synthesis of pseudo-peptides containing three amide bonds
    • Abstract: Abstract Isocyanide-based consecutive Bargellini/Ugi multicomponent reactions as a combinatorial strategy have been developed for the synthesis of new class of pseudo-peptides. Via Bargellini reaction 3-carboxamido-isobutyric acids are prepared using acetone, chloroform, sodium hydroxide, and isocyanides. Then, using Ugi multicomponent reaction strategy, pseudo-peptides containing three amide bonds are synthesized using the Bargellini reaction product, aldehydes, amines, and isocyanides. This is an efficient and eco-friendly approach for easy access to wide variety of structurally diverse, drug-like pseudo-peptides from cheap and readily available precursors in high yields.
      PubDate: 2021-01-01
  • Plasma free amino acid profiling as metabolomic diagnostic and prognostic
           biomarker in paediatric cancer patients: a follow-up study
    • Abstract: Abstract Amino acids (AAs) play a crucial role in cancer cell metabolism. Levels of 22 plasma AAs at the time of diagnosis and after treatment were established among 39 pediatric cancer patients and 33 healthy children. Glutamic acid levels decreased and tryptophan levels increased during treatment. Cancer patients presented significantly lower levels of glutamine and leucine post-treatment while levels of 12 other AAs were higher comparing to controls. Results suggest that plasma free AA profile may serve as a prognostic biomarker.
      PubDate: 2021-01-01
  • Enhanced neural differentiation of neural stem cells by sustained release
           of Shh from TG2 gene-modified EMSC co-culture in vitro
    • Abstract: Abstract As a promising cell therapy, neural crest-derived ectoderm mesenchymal stem cells (EMSCs) secrete high amounts of extracellular matrix (ECM) and neurotrophic factors, promoting neural stem cell (NSC) differentiation into neuronal lineages and aiding tissue regeneration. Additionally, the forced overexpression of secreted proteins can increase the therapeutic efficacy of the secretome. Tissue transglutaminase (TG2) is a ubiquitously expressed member of the transglutaminase family of calcium-dependent crosslinking enzymes, which can stabilize the ECM, inducing smart or living biomaterial to stimulate differentiation and enhance the neurogenesis of NSCs. In this study, we examined the neuronal differentiation of NSCs induced by TG2 gene-modified EMSCs (TG2-EMSCs) in a co-culture model directly. Two weeks after initiating differentiation, levels of the neuronal markers, tubulin beta 3 class III and growth-associated protein 43, were higher in NSCs in the TG2-EMSC co-culture group and those of the astrocytic marker glial fibrillary acidic protein were lower, compared with the control group. These results were confirmed by immunofluorescence, and laminin, fibronectin and sonic hedgehog (Shh) contributed to this effect. The results of western blot analysis and the enzyme-linked immunoassay showed that after TG2-EMSCs were co-cultured for 2 weeks, they expressed much higher levels of Shh than the control group. Moreover, the sustained release of Shh was observed in the TG2-EMSC co-culture group. Overall, our findings indicate that EMSCs can induce the differentiation of NSCs, of which TG2-EMSCs can promote the differentiation of NSCs compared with EMSCs.
      PubDate: 2021-01-01
  • Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in
           one species to that in another species on unnatural amino acid
           incorporation efficiency
    • Abstract: Abstract Genetic code expansion is a powerful technique for site-specific incorporation of an unnatural amino acid into a protein of interest. This technique relies on an orthogonal aminoacyl-tRNA synthetase/tRNA pair and has enabled incorporation of over 100 different unnatural amino acids into ribosomally synthesized proteins in cells. Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA from Methanosarcina species are arguably the most widely used orthogonal pair. Here, we investigated whether beneficial effect in unnatural amino acid incorporation caused by N-terminal mutations in PylRS of one species is transferable to PylRS of another species. It was shown that conserved mutations on the N-terminal domain of MmPylRS improved the unnatural amino acid incorporation efficiency up to five folds. As MbPylRS shares high sequence identity to MmPylRS, and the two homologs are often used interchangeably, we examined incorporation of five unnatural amino acids by four MbPylRS variants at two temperatures. Our results indicate that the beneficial N-terminal mutations in MmPylRS did not improve unnatural amino acid incorporation efficiency by MbPylRS. Knowledge from this work contributes to our understanding of PylRS homologs which are needed to improve the technique of genetic code expansion in the future.
      PubDate: 2020-12-17
  • Correction to: Plasma free amino acid profiling as metabolomic diagnostic
    • Abstract: In the original publication of the article, Table 1 has been published with an error. The data in the columns is not placed under its headings.
      PubDate: 2020-12-11
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Edinburgh, EH14 4AS, UK
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