American Journal of Agricultural and Biological Sciences
[SJR: 0.347] [H-I: 15] [10 followers] Follow
Open Access journal
ISSN (Print) 1557-4989 - ISSN (Online) 1557-4997
Published by Science Publications [28 journals]
- Using the Environmental Kuznets Curve to Study the Mutual Effects between
CO2 Emissions and Economic Growth in Jordan
Abstract: This study analyzes the dynamic relationship between economic growth and CO2 emissions in Jordan using the Environmental Kuznets Curve. The study looks at the direction of the causal relationship both in the long and short-run to determine the mutual effects between the two variables during the 1980-2010 period. Using the ARDL bound testing approach and the Vector Error Correction Model (VECM), the study shows that the results are consistent with the Environmental Kuznets Curve (EKC) hypothesis. Furthermore, the study shows the existence of a bidirectional causal relationship, both in the long and short run, between economic growth and CO2 emissions. Based on that, the authors conclude that environmental issues must be taken into consideration when formulating economic policies, as well as using environmentally friendly technologies in the manufacturing and transportation sectors.
- Development and Cultivation of Local Kidney Bean (Phaseolus Vulgaris L)
Through Breeding to use Multi gamma Irradiation Technique (Nuclear)
Abstract: Mutation on the all species of plant were generally caused by gamma or multi gamma radiation sources. The largest effect of that was occurred on chromosome and genetic factor, specially on structure and composition of chromosome and Deoxyribo Nucleic Acid (DNA). This case can be used for breeding of several important plants in Agricultural science. The objectives of this research is to develop of local kidney bean (Phaseolus Vulgaris L) that tolerant to dry condition and high production. Multi gamma radiation and selection are the main methods to use in this research and other methods comprised of observation/survey, sampling, comparison, analyzing and interpretation. The number of mutant varieties of kidney bean (superior seed of mutant) obtained in this research based on selection method are ten mutant varieties. The growth percentage rate, protein content of control and mutant variety of kidney bean were estimated respectively of 97, 22.54 and 24.21%. The production of mutant kidney bean was between 3.97 tons/ha up to 5.28 tons/ha and the average production was 4.71 tons/ha. The average production of control was 2.60 tons/ha, in order that the production was significantly increased by 44.80%.
- Phenomenon of Thrips (Thysanoptera) Attack on Chili Plant (Capsicum annuum
Abstract: Phenomenon of Thrips (Thysanoptera) Attacks on Chili Plant (Capsicum annuum L.). Thrips are insects that damage agricultural plants such as chili plants (Capsicum annuum L.). The attacks which was caused by the thrips starting from mild to severe attacks. In Jambi province there was not any report about the attacks phenomenon of thrips on chili plants. The research aimed to analyze the phenomenon of thrips attack on chili plant. The study was conducted by observing the attacks of thrips on the leaves chili plant. First, observations were done on 160 chili plants in the infested cage thrips which obtained from chili plantation, cucumbers and squash. Observations were carried out at the part of affected leaves, color, shape and location of the attack, leaf structure, chlorophyll content and the content of nitrogen, fats and carbohydrates of affected leaves. Besides, tests Elisa was also done on the affected leaves. Second, observations of thrips population and the attack percentage, as well as the abundance of predators were performed on 400 chili plants in the field experiment in Jambi University. The thrips attack was allowed to happen naturally without spraying plants by insecticide. Observations in captivity found that thrips attack occurs on the upper leaf surface, occurs at the base, the middle and the edges with silvery attack. Thrips attacks on chili plant lower chlorophyll content and damage the structure of the leaf cells. Levels of nitrogen, fats and carbohydrates of chili leaf which attacked by thrips were not significantly different from control leaves at the level of 5%. Elisa test results showed that the leaves were attacked by the thrips which derived from chili plantation and host plant do not contain Tospovirus. Results of research in the field shows that the population of thrips, the percentage of the attack and the abundance of insect predators of thrips fluctuate and the highest is at the age of 8 mst chili plants. There are 15 species of predatory thrips were found in the field experiment of Jambi University. The members of Sphecidae, Araneidae, Theridiidae and Coccinellidae were found mostly.
- The Resistance Evaluation of Dry Subtropics Brown Soils to Heavy Metal and
Oil Contamination by Biological Indicators
Abstract: Contamination of brown calcareous soils with Cr, Cu, Ni, Pb and oil leads to deterioration of their biological properties. There is a reduction in activity of catalase and dehydrogenase, an abundance of Azotobacter genus bacteria, cellulolytic activity and the length of radish roots. As a rule, there is a direct relationship between the content of contaminant in the soil and degree of deterioration of biological properties of the soil. According to the degree of negative impact on the biological properties of brown soil heavy metals form the following series: Cr > Cu > = Pb > = Ni. The pattern that chromium is more toxic and copper, lead and nickel have less and similar to each other degree of toxicity was established earlier in research conducted by the same method, with other soils in southern Russia. According to the degree of resistance brown soils are close to chestnut soils, they are less stable than chernozems and more stable than brown semi desert soils. Regional standards of Cr, Cu, Ni, Pb and oil content in brown soils of Ciscaucasia dry subtropics Ciscaucasia are proposed.
- Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase:
Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis
Abstract: An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield.
- Durum Wheat Seed Germination Response to Hydrogel Coatings and Moisture
under Drought Stress
Abstract: Technologies that can increase crop production while maintaining low pollution are essential for sustainable agriculture. This study compares the germination performance of Durum wheat seeds (Triticum durum Desf.) which were coated with synthetic coatings [polyacrylamide (PA)] and novel natural coatings [mixtures of agar/ι-carrageenan (AC)] at different moisture levels. A number of germination performance indicators were measured for each of the categories of coatings. These included: (i) % germination, (ii) radicle emergence, (iii) speed of germination, (iv) seedling length, (v) fresh seed weight and (vi) seed vigour index. Results showed that both the synthetic and natural coatings improved germination performance in the Durum wheat seeds versus the untreated seeds when non-drought (80% moisture) conditions were used. However, when 40% moisture levels were used, the natural coated seeds showed a 6% improvement in germination performance over the synthetic PA coated seeds. This improved germination performance under drought stress along with the fact that these novel AC coatings used are completely natural and environmentally friendly suggests that these novel coatings have a big future in seed coatings, especially in countries which experience drought conditions and are dependent on natural irrigation.
- Forecasting of Banana Production in Bangladesh
Abstract: In Bangladesh, banana is a very popular fruit and cultivated almost everywhere round the year. Rank of Bangladesh is 14 among the top 20 banana producing countries in the world. It is a commercial fruit, but in Bangladesh it is grown in limited area commercially. The demand of banana is increasing day by day in Bangladesh. Thus, this paper attempts to identify the Auto-Regressive Integrated Moving Average (ARIMA) model that could be used to forecast the production of banana in Bangladesh. This study considered the secondary data of yearly banana production in Bangladesh over the period 1972 to 2013. The best selected ARIMA model to forecast the banana productions in Bangladesh is ARIMA (0,2,1). The graphical comparison between the observed and forecasted banana production indicate the fitted model behaved statistically well during and beyond the estimation period.
- Thermodynamic Model of Calcium Carbonate System of Soil Solution
Abstract: Carbonate system determines the basic processes of soil formation and evolution. The aim of research was to show the importance of chemical Carbonate Calcium Equilibrium (CCE) in soil solutions. CCE causes a degree of soil solution’s saturation with CaCO3 as a factor of carbonate geochemical cycle. CCE depends on chemical composition, pH, Eh, buffering properties of liquid phase, dissolution, migration, precipitation of carbonates in the soil profile and landscape, ion exchange processes at the interface of soil solid and liquid phases. At high ionic force in soil solution are formed electrically neutral ion pairs ''''30; CaSO40, MgCO30, MgSO40 and charged ion pairs CaHCO3+, MgHCO3+, NaCO3-, NaSO4-, CaOH+, MgOH+. The object of research-saline soil of dry steppe zone of Southern Russia. This soil is moderately thick, solonized, humus 2.6%, physical clay 47.7%, clay 29.5%, CaCO3 0.15% (up to 3-10% at the depth of 0,8-1,5 m), pH = 7.8-9.0, readily soluble salts in soil solution from 0.2% in 0-5 cm to 3.5% in 70-80 cm, exchangeable cations: Ca2+ – 182 mmol kg-1, Mg2+ – 65 mmol kg-1, Na+ – 34 mmol kg-1. The soil solution was extracted from soil layers at the depth of 0-150 cm. Ion’s composition of the soil solution was determined using standard analytical methods. The mathematical model shows the real state of associated ions at high ionic strength of saline soil solution. On the basis of algorithm CCE a computer program was developed The concentration of free and associated ion form is a result of iteration beginning from analytical ion concentration considering ion material balance, linear interpolation of equilibrium constants, method of ionic pairs, laws of: Initial concentration preservation, operating masses of equilibrium system. Concentration constants of ion pair dissociation were calculated following the law of operating masses. Were determined a quantity of ion free form and the coefficient of ion association γe as a ratio of ion’s free form to its analytical content γe = Cass/Can. Because of association of ions, which differs in soil layers: The chemical equilibrium in soil solution determines high mobility of carbonates, transformation of solution, carbon sink from soil.
- Oil and Fatty Acids in Seed of Eggplant (Solanum melongena L.) and Some
Related and Unrelated Solanum Species
Abstract: The seed oil content of 305 genebank accessions of eggplant (Solanum melongena), five related species (S. aethiopicum L., S. incanum L., S. anguivi Lam., S. linnaeanum Hepper and P.M.L. Jaeger and S. macrocarpon L.) and 27 additional Solanums pecies, was determined by NMR. Eggplant (S. melongena) seed oil content varied from 17.2% (PI 63911317471) to 28.0% (GRIF 13962) with a mean of 23.7% (std. dev = 2.1) across the 305 samples. Seed oil content in other Solanum species varied from 11.8% (S. capsicoides-PI 370043) to 44.9% (S. aviculare-PI 420414). Fatty acids were also determined by HPLC in genebank accessions of S. melongena (55), S. aethiopicum (10), S. anguivi (4), S. incanum (4) and S. macrocarpon (2). In all samples examined, the predominant fatty acid was linoleic acid (18:2) followed by oleic acid (18:1) and palmitic acid (16:0). Levels of linoleic acid ranged from 57% (S. aethiopicum-PI 194166) to 74.5% (S. anguivi-PI 183357). Oleic and palmitic acid levels ranged from 11.3% (S. anguivi-PI 183357) to 25.2% (S. aethiopicum-PI 194166) and 8.4% (S. melongena-PI 115507) to 11.2% (S. melongena-PI 600912), respectively. Oil extracted from seed of S. melongena cv. Black Beauty had a pour point of -12°C, viscosities of 28.8 (40°C) and 7.3 (100°C), a viscosity index of 240, an oxidation onset temperature of 160°C and a Gardner Color of 6+.
- Heterophylly in Murraya exotica L. (Rutaceae)
Abstract: Heterophylly, the existence of different leaf types and leaflet shapes on the same plant was observed for the first time in Murraya exotica L. populations from different locations in Java, Indonesia. In each population, three types of compound leaves (paripinnate, imparipinnate and intermediate) and three types of leaflet shape (oblong, rounded and obcordate) were observed. This heterophyllous condition was found on both juvenile and adult plants. The variation in leaf type and leaflet shape may be due to the environmental conditions prevailing at the time of leaf production directing leaf development or due to developmental noise caused by random interactions between genes and the environment.
- Quality Herbage Production of Dwarf Napiergrass with Italian Ryegrass
Cropping under Digested Effluent Application in Southern Kyushu, Japan
Abstract: Digested effluent produced by a biogas-plant contains essential plant nutrients to solve the treated and disposal problems of livestock manure. Dwarf variety of late-heading type (dwarf) napiergrass as a perennial summer crop and intercropping of over-sown Italian ryegrass as an annual winter crop were applied to 3 levels of digested effluent in southern Kyushu from 2007 to 2009. Digested effluent revealed so effective fertilizer as chemical one to increase dry matter yield, wintering ability and forage quality in crude protein concentration and in vitro dry matter digestibility (digestibility) with increasing application rate. Digestibility in leaf blade of napiergrass and that in whole Italian ryegrass were positively correlated with acid detergent lignin and neutral detergent fiber concentrations, respectively, which might be mediated with reducing tiller size with defoliation proceeded. The present cropping systems have a potential under sufficient application of the effluent to achieve sustainable quality herbage production in southern Kyushu, Japan.
- Can Contract Marketing Motivate Farmers to Go Organic' Measuring the
Moderation Effect of Contract Marketing
Abstract: The demand for organic food is growing in Malaysia due to consumer concerns for food safety, sustainable development, and urban expansion. However, the share of farmers engaged in this type of agriculture in Malaysia is notably low with only 72 (0.02%) organic vegetable farmers out of a total of 272,000 farmers in the country. Therefore, it is crucial to understand why the majority of the farmers do not practice organic farming and suggest possible solutions through contract marketing as a motivating tool to enhance their participation in organic farming. In this study, a total of 329 conventional vegetable farmers were selected and interviewed through multi-stage systematic random sampling method via a structured questionnaire. Factor analysis and hierarchical multiple regression analysis has revealed that there is a moderating role by contract marketing to strengthen the relationship between attitude and public farmersâ intention to practice organic farming. This study has identified contract marketing as a component which moderates the perceived behavioral control-intention to practice organic farming. Therefore, contract farming not only shapes the attitude of farmers but it also develops the control over the chain by channeling farmersâ products to the buyers.
- Year-Round Forage Yield Stability through a System Combining Triple-Maize
Crops with Winter Barley in Kyushu, Japan
Abstract: Reduction of feeding cost for the beef and dairy cow industry is an urgent matter requiring the intensification of summer and winter forage cropping in Kyushu, Japan. Forage maize is a prominent high-yield summer crop in the region, where it used to be sown from late March to early August. Due to global warming, maize cropping in the common hot summer now easily suffers from heavy rain and typhoons and this contrasts with the need of expanding the growth periods suitable for maize cropping. This study tested the cropping of triple (spring, summer and autumn) maize crops with winter barley to determine its suitability to be cultivated in the region. Winter barley, sown into inter-rows of the previous row crops in mid-November, was harvested from late February to late March, when spring maize was sown earlier than the current season, after the danger of late frost has passed. Summer maize was sown from early June to early July and autumn maize which was sown from late August to mid-September was harvested in mid-to late November. Winter barley yielded 900-1090 g dry matter (DM)/m2 with 22% ear DM. Early-planted spring maize which was sown in early March yielded 1600-1880 g DM/m2 with 16-28% ear DM and summer maize yielded 900-1280 g DM/m2 with 19-39% ear DM. However, the autumn maize yield varied from 130-300 g DM/m2 without seed maturation to 740-880 g DM/m2 with 51-54% ear DM due to typhoon and cool weather damage. Therefore, triple maize with winter barley cropping can produce a yield of more than 4000 g DM/m2, especially from 2600 g DM/m2 from winter barley and spring maize crops sown in early March, leading to the conclusion that spring maize sown in early March can be harvested without high risk from weather disasters in the region (290 words ≤ 300 words, upper-limit).
- SSR Analysis of Maternal and Paternal Lines Selected in the Don Region
Abstract: Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf lines with use of 11 SSR markers. It is shown that the level of genetic diversity of microsatellite loci of CMS lines is 1.2 times lower than that of Rf lines. The average number of alleles per SSR locus for maternal lines also lowers than for paternal lines. In addition, CMS lines and Rf lines differ in the frequency of alleles of SSR loci. Thus, the discriminatory capacity of the studied marker system for identification of cultivated sunflower lines was quite high and is suitable for certification of the lines.
- GIS Mapping of Basal Stem Rot Disease in Relation to Soil Series Among Oil
Abstract: Basal Stem Rot (BSR) is a disease caused by fungus which affect oil palm tree. This study aims to visualize the geographical distribution of BSR incidence among oil palm smallholders in Selangor using Geographical Information System (GIS) and its relationship to the type of soil series. The geographical distribution of BSR incidence of smallholders in Selangor were mapped using 168 Global Positioning System (GPS) coordinate of possible infected area. The methodology followed in finding the relationship between BSR and soil series by assigning percentage of BSR incidence based on type of soil series. Majority of the BSR incidences were found to be confined to the Selangor-Kangkong (Aeric Tropic Fluvaquent-Typic sulfuric tropic fluvaquent) soil series followed on Peat (Typic Fibric Tropohemist) soil then Kranji (Typic Sulfaquents) soil series and finally the Briah-Organic Clay and Muck (Typic Tropic Fluvaquent) soil series. Distribution pattern of the BSR revealed that most of the incidences were confined along the coastal area which might be due to the planting of oil palm in previous coconut stands. Low pH of Selangor (Aeric Tropic Fluvaquent) and Peat (Typic Fibric Tropohemist) soil series shows a higher BSR incidence while high pH of Kranji (Typic Sulfaquents) and Briah (Typic Tropic Fluvaquent) soil series shows slightly lower BSR incidence. From this study, it can be concluded that the distribution of BSR were mostly confined to the coastal areas which might be due to the planting of oil palm in previous coconut stands rather than any relationship with the type of soil series. This distribution map of BSR incidence can be used to deliver effective extension services regarding BSR to smallholders with a specified target based on location and soil series.
- Models of Winter Wheat Yield Based on Calcareous Chernozem Fertility
Abstract: The use of polyelement diagnostics and regression analysis for predicting the yield of winter wheat at different stages of vegetation is considered. It has been established that the prediction and integrated assessment of grain yield and quality should be performed with consideration for the balance of macro-and micronutrients in the grain and the aboveground biomass of plants. Possibility of predicting the contents of macro-and micronutrients in wheat grain from the chemical composition of plants at the shooting stage has been revealed. Scientifically based recommendations are presented for managing the grain yield and quality of winter wheat.
- Compaction Properties of Silty Soils in Relation to Soil Texture, Moisture
Content and Organic Matter
Abstract: Soil compaction is recognized as an increasingly challenging problem with regard to root growth and yield reduction in agricultural production in Thailand. The aim of this research was to study the effect of soil texture, moisture content and organic matter on the compaction properties of silty soils. Three soils with different silt contents were studied-loam, silty clay loam and silt loam. The study showed that compacting silt loam soil using the modified Proctor test resulted in a higher bulk density than from the oedometer test at 200 kPa. Statistical analysis showed that soil type and the moisture content significantly affected both the bulk density and penetration resistance. However, organic matter showed a significant effect only on the bulk density. The bulk density increased with increased moisture content. The bulk densities of compacted soils were higher in the order of loam, silt loam and silty clay loam. The penetration resistance seemed to increase with increased silt content. The differences in the bulk density and penetration resistance among these three soils decreased as the moisture content increased. Adding organic matter at 5% by weight to soils resulted in lower bulk densities of the soil using the oedometer test, with reductions of 3.9, 3.8 and 7.7% for loam, silty clay loam and silt loam, respectively. In order to avoid effects on root growth, it is recommended that machines with 200 kPa of tire contact pressure should only work in fields with a moisture content not exceeding 20.27, 18.74 and 17.88% for loam, silt loam and silty clay loam, respectively.
- Can Anthocyanins be Part of the Metal Homeostasis Network in Plant?
Abstract: Anthocyanins are a class of flavonoids with a high level ofdiversification and likely the most studied pathway of secondary metabolism in Plantae.Anthocyanins have raised a growing interest due to the huge variability oftheir chemical structures and the more new anthocyanins are isolated fromplants, the more questions on their evolutionary and ecological meaning theyraise. Antioxidant, photoprotection against high light and UV, defence againstherbivores and pathogens, attraction of pollinator are only some proposedbiological functions for those versatile compounds. Anthocyanins have also beenfound complexed with metal ions either in flower pigments (commelin andprotocyanin) or in leaves and stems. Due to the potentiality of anthocyanins tochelate to metals, their involvement in the attenuation of metal toxicity hasbeen recently proposed. Conversely, the ability of plants to remobilize metalions from stored metal-anthocyanin complexes when plants experience a period oftransient metal shortage has never been investigated before. The aim of this paperis to support the hypothesis that the anthocyanin-metal interactions mightrepresent a further ecological role for these pigments and also thatanthocyanins can be part of the complex network of metal homeostasis in plant.
- Introgression the SalTol QTL into the Elite Rice Variety of Russia by
Abstract: The present work is devoted to investigation of the possibility to apply methods of Marker-Assisted Selection (MAS) to introgression the SalTol QTL into genotypes of elite Russian varieties of rice. It was shown that microsatellite markers Rm493, may be effectively used to control transfer the SalTol QTL genes into Russian populations of rice. Based on the highly productive variety “Novator”, we obtained the lines Nov-129 and Nov-148 carrying loci SalTol in homozygous state. The lines Nov-129 and Nov-148 are used as an improved salt tolerance donor source to obtain hybrids tolerance to salinity.
- Effects of Cement Dust on the Physiological Activities of Arabidopsis
Abstract: Air pollution exerts detrimental effects on plant ecosystems and restricts agricultural productivity. Cement dust is considered to be one of the most harmful air pollutants of industrial origin and is a limiting factor for plant growth and productivity. This study aims to uncover the impacts of cement dust on photosynthetic pigments, protein content, hydrogen peroxide (H2O2) accumulation, lipid per oxidation and antioxidant enzyme activities of Arabidopsis thaliana. In this study, Arabidopsis plants were dusted with cement at a rate of 1.5 g per 1-m2 area and measurements were undertaken at 10 days after cement dust application. Treatment with cement dust resulted insignificant reductions in chlorophyll content and total soluble protein accumulation. Neither carotenoid nor starch content of Arabidopsis plants was affected by exposure to cement dust, whereas protease activity was significantly enhanced in cement dust-treated plants. Furthermore, exposure to cement dust significantly enhanced the production of H2O2, a product of oxidative stress, in the leaves of Arabidopsis plants. Moreover, Malondialdehyde (MDA) content, a product of lipid per oxidation, significantly increased after exposure to cement dust. In response to cement dust, activities of scavenging enzymes such as Ascorbate Peroxides (APX), Superoxide Dismutase (SOD) and Guaiacol Peroxidase (GPX) increased, whereas the activity of Catalase (CAT) activity decreased. The present results suggest that cement dust induced oxidative stress in Arabidopsis plants through the generation of Reactive Oxygen Species (ROS), induction of lipid peroxidation and up regulation of antioxidant enzyme activities.
- Genetic Relationship among Wild Medicinal Genotypes of Ziziphora canescens
Benth. and Ziziphora tenuior L. and Detection of Genetic Variations
Resulted From Tissue Culture, Salinity and pH Media
Abstract: Ziziphora tenuior L. and Ziziphora canescens Benth. are two of plants which using in folk medicine in the Kalamoon Mountains areas of Syria for cough, stomachache and dysentery. Samples of Ziziphora genotypes were collected from four different locations (Assal Al-Ward, Yabroud, Qarah and Maaraba), 20 RAPD and 12 ISSR primers were used to assess the genetic diversity of 10 genotypes. Amplified fragments were polymorphic with percentage 93.4% and 100% when RAPD and ISSR markers were used respectively. The first cluster depending on RAPD data formed by the grouping of all Ziziphora tenuior L. and the second cluster formed by grouping of all Ziziphora canescens Benth. genotypes. While the first cluster based on ISSR data formed by two genotypes Ziziphora tenuior L. (Maaraba), the second cluster formed by three genotypes Ziziphora tenuior L. and five genotypes Ziziphora canescens Benth. (Assal Al-Ward, Yabroud and Qarah). ISSR markers were recorded a high degree of biodiversity among Ziziphora tenuior L. genotype collected from Maaraba and other genotypes. Genetic stability of Ziziphora tenuior L. and Ziziphora canescens Benth. was confirmed among mother plant and shoots that underwent one to nine cycles of in vitro subculturing by RAPD markers that produced monomorphic bands, while ISSR bands were polymorphic especially in 7,8 and 9 subcultures. Also Genetic variations of Ziziphora canescens Benth. that resulted from salinity (1,2,3,4 and 5 g/L) and pH (7,8 and 9) media were detected, both of RAPD and ISSR bands were polymorphic compared with control except some RAPD primers produced monomorphic bands. Callus from Ziziphora tenuior L. that induction on different media (MS + 1.5 mg/L NAA + 0.5 mg/L Kin) or (MS + 2 mg/L IBA + 0.5 mg/L Kin) showed high variations compare with micropropagated plants from apical mirestem on media (MS + 1 mg/L Kin + 0.1 mg/L NAA).
- The Influence of Mineral Fertilizers on the Chemical Composition of
Verdurous Masses of Forder Crops
Abstract: This article presents the results of sorghum and pearl millet studies conducted in order to develop the technology of their cultivation as a new forage crops for livestock in arid areas of Kazakhstan. The article presents data on the effect of seeding rates and term of sowing on seeds germination and survival of plants and a crop of verdurous masses of sorghum and pearl millet against the background of watering. The optimal seeding rate and sowing of sorghum and pearl millet in saline soils of rice irrigation systems are determined in terms of the ecological crisis in Aral Sea region, Kazakhstan.