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CHEMISTRY (658 journals)                  1 2 3 4 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 14)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 31)
ACS Applied Polymer Materials     Hybrid Journal  
ACS Catalysis     Hybrid Journal   (Followers: 54)
ACS Chemical Neuroscience     Hybrid Journal   (Followers: 22)
ACS Combinatorial Science     Hybrid Journal   (Followers: 21)
ACS Macro Letters     Hybrid Journal   (Followers: 29)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 46)
ACS Nano     Hybrid Journal   (Followers: 364)
ACS Photonics     Hybrid Journal   (Followers: 15)
ACS Symposium Series     Full-text available via subscription   (Followers: 1)
ACS Synthetic Biology     Hybrid Journal   (Followers: 24)
Acta Chemica Iasi     Open Access   (Followers: 6)
Acta Chimica Slovaca     Open Access   (Followers: 2)
Acta Chimica Slovenica     Open Access   (Followers: 1)
Acta Chromatographica     Full-text available via subscription   (Followers: 8)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 7)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 8)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 9)
Adsorption Science & Technology     Open Access   (Followers: 7)
Advanced Functional Materials     Hybrid Journal   (Followers: 63)
Advanced Science Focus     Free   (Followers: 5)
Advances in Chemical Engineering and Science     Open Access   (Followers: 85)
Advances in Chemistry     Open Access   (Followers: 28)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 20)
Advances in Drug Research     Full-text available via subscription   (Followers: 26)
Advances in Environmental Chemistry     Open Access   (Followers: 8)
Advances in Enzyme Research     Open Access   (Followers: 11)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 9)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 17)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 11)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 28)
Advances in Nanoparticles     Open Access   (Followers: 18)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Polymer Science     Hybrid Journal   (Followers: 49)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 19)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Science and Technology     Full-text available via subscription   (Followers: 13)
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 5)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 8)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Al-Kimia : Jurnal Penelitian Sains Kimia     Open Access  
Alchemy : Journal of Chemistry     Open Access   (Followers: 3)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
Alotrop     Open Access  
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 71)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 22)
American Journal of Chemistry     Open Access   (Followers: 34)
American Journal of Plant Physiology     Open Access   (Followers: 13)
American Mineralogist     Hybrid Journal   (Followers: 15)
Anadolu University Journal of Science and Technology A : Applied Sciences and Engineering     Open Access  
Analyst     Full-text available via subscription   (Followers: 37)
Angewandte Chemie     Hybrid Journal   (Followers: 192)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 282)
Annales Universitatis Mariae Curie-Sklodowska, sectio AA – Chemia     Open Access   (Followers: 1)
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 4)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 4)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 8)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 15)
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 2)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 9)
Applied Spectroscopy     Full-text available via subscription   (Followers: 26)
Applied Surface Science     Hybrid Journal   (Followers: 33)
Arabian Journal of Chemistry     Open Access   (Followers: 6)
ARKIVOC     Open Access   (Followers: 1)
Asian Journal of Biochemistry     Open Access   (Followers: 3)
Asian Journal of Chemistry and Pharmaceutical Sciences     Open Access   (Followers: 2)
Atomization and Sprays     Full-text available via subscription   (Followers: 5)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 4)
Avances en Quimica     Open Access  
Biochemical Pharmacology     Hybrid Journal   (Followers: 11)
Biochemistry     Hybrid Journal   (Followers: 396)
Biochemistry Insights     Open Access   (Followers: 7)
Biochemistry Research International     Open Access   (Followers: 7)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 11)
Bioinspired Materials     Open Access   (Followers: 5)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access   (Followers: 2)
Biomacromolecules     Hybrid Journal   (Followers: 25)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 10)
Biomedical Chromatography     Hybrid Journal   (Followers: 6)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 6)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 180)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 92)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 17)
Biosensors     Open Access   (Followers: 2)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 1)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 25)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 3)
Cakra Kimia (Indonesian E-Journal of Applied Chemistry)     Open Access  
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 2)
Canadian Journal of Chemistry     Hybrid Journal   (Followers: 12)
Canadian Mineralogist     Full-text available via subscription   (Followers: 7)
Carbohydrate Research     Hybrid Journal   (Followers: 24)
Carbon     Hybrid Journal   (Followers: 72)
Catalysis for Sustainable Energy     Open Access   (Followers: 10)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 10)
Catalysis Science and Technology     Hybrid Journal   (Followers: 10)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 14)
Cellulose     Hybrid Journal   (Followers: 14)
Cereal Chemistry     Full-text available via subscription   (Followers: 5)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 2)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 23)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 74)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 27)
Chemical Physics Letters : X     Open Access   (Followers: 2)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Hybrid Journal   (Followers: 23)
Chemical Reviews     Hybrid Journal   (Followers: 228)
Chemical Science     Open Access   (Followers: 32)
Chemical Technology     Open Access   (Followers: 46)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 5)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 56)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 21)
ChemInform     Hybrid Journal   (Followers: 8)
Chemistry     Open Access  
Chemistry & Biodiversity     Hybrid Journal   (Followers: 7)
Chemistry & Biology     Full-text available via subscription   (Followers: 32)
Chemistry & Industry     Full-text available via subscription   (Followers: 8)
Chemistry - A European Journal     Hybrid Journal   (Followers: 188)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 16)
Chemistry and Materials Research     Open Access   (Followers: 21)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Open Access   (Followers: 3)
Chemistry Letters     Full-text available via subscription   (Followers: 45)
Chemistry of Heterocyclic Compounds     Hybrid Journal   (Followers: 4)
Chemistry of Materials     Hybrid Journal   (Followers: 282)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 10)
Chemistry World     Full-text available via subscription   (Followers: 21)
Chemistry-Didactics-Ecology-Metrology     Open Access   (Followers: 1)
ChemistryOpen     Open Access   (Followers: 1)
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 3)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 15)
Chemosensors     Open Access  
ChemPhysChem     Hybrid Journal   (Followers: 12)
ChemPlusChem     Hybrid Journal   (Followers: 2)
ChemTexts     Hybrid Journal  
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 12)
Chromatographia     Hybrid Journal   (Followers: 22)
Chromatography     Open Access   (Followers: 3)
Chromatography Research International     Open Access   (Followers: 5)
Clay Minerals     Hybrid Journal   (Followers: 10)
Cogent Chemistry     Open Access   (Followers: 2)
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 11)
Colloids and Interfaces     Open Access  
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 8)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 4)
Combustion Science and Technology     Hybrid Journal   (Followers: 24)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Communications Chemistry     Open Access   (Followers: 2)
Composite Interfaces     Hybrid Journal   (Followers: 8)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 1)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 2)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 9)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 13)
Computational Chemistry     Open Access   (Followers: 3)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 10)
Coordination Chemistry Reviews     Full-text available via subscription   (Followers: 4)
Copernican Letters     Open Access   (Followers: 1)
Corrosion Series     Full-text available via subscription   (Followers: 7)
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 8)
Croatica Chemica Acta     Open Access  
Crystal Structure Theory and Applications     Open Access   (Followers: 4)
CrystEngComm     Full-text available via subscription   (Followers: 13)
Current Catalysis     Hybrid Journal   (Followers: 2)
Current Chromatography     Hybrid Journal  
Current Green Chemistry     Hybrid Journal   (Followers: 2)
Current Metabolomics     Hybrid Journal   (Followers: 6)
Current Microwave Chemistry     Hybrid Journal  
Current Opinion in Colloid & Interface Science     Hybrid Journal   (Followers: 10)
Current Opinion in Molecular Therapeutics     Full-text available via subscription   (Followers: 13)
Current Research in Chemistry     Open Access   (Followers: 9)

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Similar Journals
Journal Cover
Colloids and Surfaces B: Biointerfaces
Journal Prestige (SJR): 1.071
Citation Impact (citeScore): 4
Number of Followers: 8  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0927-7765
Published by Elsevier Homepage  [3182 journals]
  • Injected laquinimod D-α-tocopheryl polyethylene glycol-1000 succinate
           polymeric micelles for the treatment of inflammatory bowel disease
    • Abstract: Publication date: Available online 19 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Lifen Wang, Pengpeng Xue, Mengqi Tong, Rui Chen, Waigeng Yang, Deli ZhuGe, Jiandong Yuan, Qing Yao, Yingzheng Zhao, Helin Xu Inflammatory bowel diseases (IBDs) are chronic relapsing disorders of the gastrointestinal tract characterized pathologically by intestinal inflammation and epithelial injury. Laquinimod (LAQ), a poorly water-soluble compound, was proved to be effective for colitis remission at low dose of 0.5 mg/kg in patients with Crohn’s disease. Due to its extremely low solubility in water, it was difficult to develop an injectable liquid dosage form. Herein, D-α-Tocopheryl polyethylene glycol-1000 succinate (TPGS) polymeric micelles were developed as a delivery vehicle of LAQ for the management of inflammatory bowel disease. Using the LAQ/TPGS ratio of 1:100, LAQ-loaded micelles were successfully prepared by thin-film dispersion method. The solubility of LAQ in water was significantly increased from 10.5 μg/mL in pure water to 500 μg/mL in TPGS micelles. LAQ-loaded micelles of TPGS exhibited the fine particle size of 34.6 nm and Zeta potential of -0.67 mV. Moreover, the good stability of LAQ-loaded micelles in physiology-mimicking medium was confirmed by detecting their particle size, zeta potential and leakage of the loading drug. Therapeutic effect of LAQ-loaded micelles on DSS-induced mice was proved by detecting DAI score, colon length and loss of body weight. Moreover, the morphology and colonic mucosal barrier of the injured colon of DSS-induced mice was largely recovered after treatment with LAQ-loaded micelles. Meanwhile, the inflammation of colitis colon was also obviously alleviated by LAQ-loaded micelles. Conclusively, polymeric micelles of TPGS may be a promising delivery vehicle of LAQ for the management of inflammatory bowel disease.Graphical abstractGraphical abstract for this article
  • Effect of a dirhamnolipid biosurfactant on the structure and phase
           behaviour of dimyristoylphosphatidylserine model membranes
    • Abstract: Publication date: Available online 19 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Alfonso Oliva, José A. Teruel, Francisco J. Aranda, Antonio Ortiz Rhamnolipids are bacterial biosurfactants containing one or two rhamnose rings and a hydrophobic hydrocarbon portion. These compounds are mainly isolated from Pseudomonas spp culture media, and have been shown to present outstanding biological activities. A number of experimental works have shown that the interaction of rhamnolipids with target membranes could play a role in these actions. Therefore the study of the interaction of purified rhamnolipids with the various phospholipid components of biological membranes is of great interest. This paper shows the phase behaviour of mixtures of 1,2-dimyristolylphosphatidylserine (DMPS) with a dirhamnolipid (diRL) fraction produced by P. aeruginosa. This experimental approach has been based on the use of physical techniques such as Differential Scanning Calorimetry (DSC) and Fourier-Transform Infrared Spectroscopy (FTIR). DSC indicated that the presence of increasing concentrations of diRL in the bilayer resulted in a progressive broadening of the gel to liquid-crystalline phase transition of DMPS. In addition a complex thermal behaviour was observed, with the presence of more than one transition at higher concentrations of the biosurfactant, indicating phase separation. FTIR showed that diRL increased the proportion of gauche rotamers of DMPS, thus affecting acyl chain order. The change in the frequency of the carboxylate stretching band of DMPS observed upon interaction with diRL pointed toward changes in the local environment of the polar headgroup of the phospholipid, resulting in a modification of its conformation or insertion within the bilayer. This result was corroborated by the effect of diRL on the carbonyl and phosphate stretching bands of DMPS, showing an increase of the hydration both in the gel and in the liquid-crystalline phase. Molecular Dynamics (MD) simulations gave further support to the experimental results, showing diRL cluster formation as well as an augmented exposition of DMPS to the water layer in the presence of the biosurfactant.Graphical Graphical abstract for this article
  • Effect of molecular structure of eco-friendly glycolipid biosurfactants on
           the adsorption of hair-care conditioning polymers
    • Abstract: Publication date: Available online 19 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Laura Fernández-Peña, Eduardo Guzmán, Fabien Leonforte, Ana Serrano-Pueyo, Krzysztof Regulski, Lucie Tournier-Couturier, Francisco Ortega, Ramón G. Rubio, Gustavo S. Luengo Pseudo-binary mixtures of different glycolipids, four different rhamnolipids (RL) and an alkyl polyglucoside (APG), with poly(diallyl-dimethylammonium chloride) (PDADMAC) have been studied in relation to their adsorption onto negatively charged surfaces to shed light on the impact of the molecular structure of biodegradable surfactants from natural sources (instead of synthetic surfactant, such as sodium laureth sulfate) on the adsorption of hair-care formulations. For this purpose, the self-assembly of such mixtures in aqueous solution and their adsorption onto negatively charged surfaces mimicking the negative charge of damaged hair fibres have been studied using experiments and self-consistent field (SCF) calculations. The results show that the specific physico-chemical properties of the surfactants (charge, number of sugar rings present in surfactant structure and length of the hydrocarbon length) play a main role in the control of the adsorption process, with the adsorption efficiency and hydration being improved in relation to conventional sulfate-based systems for mixtures of PDADMAC and glycolipids with the shortest alkyl chains. SCF calculations and Energy Dispersive X-Ray Spectroscopy (EDS) analysis on real hair confirmed such observations. The results allow one to assume that the characteristic of the studied surfactants, especially rhamnolipids, conditions positively the adsorption potential of polyelectrolytes in our model systems. This study provides important insights on the mechanisms underlying the performance of more complex but natural and eco-friendly washing formulations.Graphical abstractGraphical abstract for this article
  • Calorimetric approach to understand pH and salt influence on the
           adsorption mechanism of lysozyme to a traditional cation exchanger
    • Abstract: Publication date: Available online 18 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): F.S. Marques, G.L. Silva, M.E. Thrash, A.C. Dias-Cabral Built upon our interest in illustrating the complexity of protein adsorption onto chromatographic supports and to understand the rule of nonspecific interactions in the ion exchange adsorption process, a traditional model system (lysozyme – carboxymethyl cellulose) was used to determine the charge influence during biomolecule adsorption. Flow microcalorimetry (FMC) was exploit as a dynamic technique that provides adsorption and desorption heat signals for a specific system, permitting an improved understanding of the driving forces and mechanisms involved during the interaction. For this purpose, measurements were made at pH 8 at both absence and presence of salt (NaCl 50 mM) and compared with previous studies performed at pH 5. Distinct FMC profiles were observed regarding pH. For most of the experiments, two exothermic heat signals are observed at pH 8 while at pH 5 one endothermic and one exothermic peak are shown. This difference was justified with a less energy demanding for desolvation at pH 8. Lysozyme adsorption was shown to be a multi-step process involving desolvation, primary protein adsorption and secondary adsorption after reorientation with distinct contributions to the overall energy. At pH 8, the exothermic contribution to the adsorption process is lower compared to pH 5, which is justified by the lower charge density that lysozyme presents at pH 8 compared to pH 5.Graphical abstractGraphical abstract for this article
  • Effects of primary amine-based coatings on microglia internalization of
    • Abstract: Publication date: Available online 17 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Emanuele Mauri, Pietro Veglianese, Simonetta Papa, Arianna Rossetti, Massimiliano De Paola, Alessandro Mariani, Zbyšek Posel, Paola Posocco, Alessandro Sacchetti, Filippo Rossi Nanogels represent a pivotal class of biomaterials in the therapeutic intracellular treatment of many diseases, especially those involving the central nervous system (CNS). Their biocompatibility and synergy with the biological environment encourage their cellular uptake, releasing the curative cargo in the desired area. As a main drawback, microglia are generally able to phagocytize any foreign element overcoming the blood brain barrier (BBB), including these materials, drastically limiting their bioavailability for the target cells. In this work, we investigated the opportunity to tune and therefore reduce nanogel internalization in microglia cultures, exploiting the orthogonal chemical functionalization with primary amine groups, as a surface coating strategy. Nanogels are designed by following two methods: the direct grafting of aliphatic primary amines and the linkage of -NH2 modified PEG on the nanogel surface. The latter synthesis was proposed to evaluate the combination of PEGylation with the basic nitrogen atom. The achieved results indicate the possibility of effectively modulating the uptake of nanogels, in particular limiting their internalization using the PEG-NH2 coating. This outcome could be considered a promising strategy for the development of carriers for drugs or gene delivery that could overcome microglia scavenging.Graphical abstractGraphical abstract for this article
  • A multifunctional nanoplatform based on MoS2-nanosheets for targeted drug
           delivery and chemo-photothermal therapy
    • Abstract: Publication date: Available online 17 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yanbo Yang, Jianrong Wu, David H. Bremner, Shiwei Niu, Yu Li, Xuejing Zhang, Xiaotian Xie, Li-Min Zhu Synergistic tumor treatment has recently attracted more and more attention due to its remarkable therapeutic effect. Herein, a multifunctional drug delivery system based on hyaluronic acid (HA) targeted dual stimulation responsive MoS2 nanosheets (HA-PEI-LA-MoS2-PEG, HPMP) for active interaction with CD44 receptor positive MCF-7 cells is reported. Melanin (Mel), a new type of photothermal agent and doxorubicin (DOX) are both loaded onto the HPMP nanocomposite and can be released by mild acid or hyperthermia. The prepared HPMP nanocomposite has a uniform hydrodynamic diameter (104 nm), a high drug loading (944.3 mg.g-1 HPMP), a remarkable photothermal effect (photothermal conversion efficiency: 55.3%) and excellent biocompatibility. The DOX release from HPMP@(DOX/Mel) can be precisely controlled by the dual stimuli of utilizing the acidic environment in the tumor cells and external laser irradiation. Meanwhile, loading of Mel onto the surface can enhance the photothermal effect of the MoS2 nanosheets. In vitro experiments showed that the HPMP@(DOX/Mel) nanoplatform could efficiently deliver DOX into MCF-7 cells and demonstrated enhanced cytotoxicity compared to that of the non-targeted nanoplatform. In vivo experiments in a breast cancer model of nude mice further confirmed that the HPMP@(DOX/Mel) significantly inhibited tumor growth under near infrared (NIR) laser irradiation, which is superior to any single therapy. In summary, this flexible nanoplatform, based on multi-faceted loaded MoS2 nanosheets, exhibits considerable potential for efficient pH/NIR-responsive targeted drug delivery and chemo-photothermal synergistic tumor therapy.Graphical abstractGraphical abstract for this article
  • Nanotubes and nano pores with chitosan construct on TiZr serving as drug
    • Abstract: Publication date: 1 January 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 185Author(s): Andrei Bogdan Stoian, Ioana Demetrescu, Daniela Ionita Taking into account that modified and non modified TiZr alloys have a chance as alternatives for Ti as implant materials, this paper is focused on the elaboration and characterization of TiZr hybrid nanostructures (nanopores and nanotubes) loaded with gentamicin (GS) and covered with chitosan. FT-IR analysis permitted structure and corresponding bands identification. Scanning electronic microscopy (SEM) was used for morphology analysis and nanostrucure dimensions evaluations. The surface analysis was completed with roughness measurements, contact angle determinations and surface energy evaluations. The amount of drug released in both cases was measured using ultraviolet–visible spectroscopy (UV/Vis). The in-vitro drug release profile was applied to three kinetic mathematical models (Korsmeyer-Peppas, Peppas-Sahlin and Linder-Lippold). Using the experimental data on the three simulation models, the Lindner-Lippold was found to be the best suited for the transport mechanism dominated by Fickian diffusion. It was observed that the same quantity of gentamicin (approx. 95%) will be released in 10 days from nanopores and in 21 days from nanotubes, establishing in both cases longer term release as an expression of better targeted treatment of bone and osteomyelitis.Graphical abstractGraphical abstract for this article
  • Thermoresponsive anionic copolymer brush-grafted surfaces for cell
    • Abstract: Publication date: 1 January 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 185Author(s): Kenichi Nagase, Naho Uchikawa, Tadashi Hirotani, Aya Mizutani Akimoto, Hideko Kanazawa Cell separation methods that do not require surface modification of cells are needed in tissue engineering and regenerative medicine. We developed thermoresponsive anionic polymer brushes for cell separation without modification of the cell surfaces. Copolymer brush poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-tert-butyl acrylate, P(NIPAAm-co-tBAAm-co-tBA), was prepared on a cover glass plate through activator regenerated by electron transfer atom transfer radical polymerization (ARGET-ATRP). The tert-butyl group of the copolymer brush was then deprotected and a P(NIPAAm-co-tBAAm-co-acrylic acid (AAc)) brush-modified glass surface was obtained. ARGET-ATRP synthesis achieved polymers with low polydispersity. The negative surface charge of the polymer brush-modified substrates was evaluated using zeta potential measurements and the phase transition temperature of the polymer was modulated between 37–20 °C to perform cell adhesion and detachment, respectively. The adhesion and detachment behavior of cells used in cardiovascular tissue engineering on the thermoresponsive anionic polymer brushes was investigated. Normal human umbilical vein endothelial cells (HUVEC) exhibited prompt detachment from the thermoresponsive anionic polymer brush surfaces. In addition, normal human aortic smooth muscle cells (SMC) exhibited relatively high adhesion on thermoresponsive anionic polymer brush-modified surfaces compared with those modified with thermoresponsive polymer brushes without anionic groups. By utilizing the difference in the cell adhesion and detachment properties of the cell types, a mixture of HUVEC and SMC was separated simply by altering the applied temperature. This result indicated that the prepared thermoresponsive anionic polymer brush-modified glass surface could be used as a tool for the separation of cells in cardiovascular tissue engineering.Graphical abstractGraphical abstract for this article
  • Fe3O4 composited with MoS2 blocks
           horizontal gene transfer
    • Abstract: Publication date: 1 January 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 185Author(s): Honggui Wang, Huachen Qi, Shujun Gong, Zhihai Huang, Chuang Meng, Ya Zhang, Xiang Chen, Xin’an Jiao In this study, we found that Fe3O4 promoted horizontal gene transfer (HGT), but when Fe3O4 was composited with MoS2, the Fe3O4@MoS2 nanocomposite interacting with bacteria significantly blocked the HGT in the conjugation system. qPCR was used to analyze the expression of genes belonging to the chromosome and plasmid in the conjugation system. Results demonstrated that Fe3O4@MoS2 inhibited conjugation by promoting the expression of the global regulatory gene (trbA) and inhibiting the expression of conjugative transfer genes involved in mating pair formation (traF, trbB), DNA replication (trfA), and porins (outer membrane protein (omp) A and ompC). All of these genes are related to the permeability of the cell membrane, except for trfA. The results showed that Fe3O4@MoS2 interacted with bacteria to decrease their permeability against exogenous DNA. MoS2 may play an essential role in the HGT-inhibiting activity of Fe3O4@MoS2. This study highlights the diverse biological properties of nano-materials and provides clues for nano-scientists to develop environmentally friendly materials.Graphical abstractThe solid arrow means “promote”, and the T arrow represents “inhibit”.Graphical abstract for this article
  • Polymerized graphene oxide/MnCe0.5Fe1.5O4 nanoferrofluid as a T2- and
           T2*-weighted contrast agent for magnetic resonance imaging
    • Abstract: Publication date: 1 January 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 185Author(s): Narges Torkashvand, Nahid Sarlak In this study synthesis of graphene oxide-poly citric acid/MnCe0.5Fe1.5O4 nanoferrofluid with a simple method and its ability for enhancing the contrast of magnetic resonance images was reported. The co-precipitation method has been used for the production of MnCe0.5Fe1.5O4 nanoparticles with a size distribution of 25–40 nm, which has shown a very good superparamagnetic property with saturation magnetization (Ms) 53.6 emu g−1. Graphene oxide (GO) was prepared by a modified Hummers method and polymerized with citric acid to produce GO-PCA. The nanoparticles were loaded on the GO-PCA surface to produce nanoferrofluid that showed excellent colloidal stability, extra dispersibility, and good magnetic properties with Ms equal to 47.8 emu g−1. This nanoferrofluid has an increased effect on the contrast of T2 and T2*-weighted images, with the values 109.15 and 180.23 mM−1 s−1 for r2 and r2*, respectively at 3.0 T. The cellular uptake evaluation revealed adequate uptake for HeLa cell lines.Graphical abstractGraphical abstract for this article
  • Self-assembling Dextran prodrug for redox- and pH-responsive co-delivery
           of therapeutics in cancer cells
    • Abstract: Publication date: 1 January 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 185Author(s): Manuela Curcio, Giuseppe Cirillo, Alessandro Paolì, Giuseppina Daniela Naimo, Loredana Mauro, Diana Amantea, Antonella Leggio, Fiore Pasquale Nicoletta, Francesca Iemma Self-assembling prodrug containing pH- and redox-responsive functional groups was prepared by covalent conjugation of Doxorubicin (Dox) and lipoic acid (LA) to a polyaldehyde Dextran (PAD). The resultant amphiphilic DoxPADLA forms, in a single step, hemocompatible vesicular systems able to respond to intracellular signals without using external crosslinking agents. Camptothecin (CPT) was encapsulated exploiting the hydrophobic interactions with the vesicle membrane, and release experiments, carried out in media mimicking the physiological and endolysosomial compartments, in the absence or presence of Glutathione, proved the ability of the system to modulate drug release in relation to the variation of pH and redox potential. Cytotoxicity assays and confocal experiments demonstrated the efficacy of the vesicle formulation in enhancing the synergistic anticancer effect of the delivered Dox and CPT and a rapid and significant internalization of the carrier in cancer cells.Graphical abstractGraphical abstract for this article
  • Bottom-up synthesis of nitrogen and oxygen co-decorated carbon quantum
           dots with enhanced DNA plasmid expression
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Amir Yadegari, Jafar Khezri, Sadaf Esfandiari, Hossein Mahdavi, Ali Asghar Karkhane, Reza Rahighi, Rashid Heidarimoghadam, Lobat Tayebi, Ehsan Hashemi, Abbas Farmany In this paper, a bottom-up hydrothermal route is reported for the synthesis of oxygen and nitrogen co-decorated carbon quantum dots (CQDs) using ammonium hydrogen citrate (AHC) as a single precursor. DLS data approved the formation of 4.0 nm (average size) CQDs. XRD pattern shows the interlayer spacing (002) of 3.5 Å for CQDs, which is exactly the same as that of crystalline graphite. XPS and FTIR spectra verified the formation of oxygen and nitrogen functional groups on the CQDs surface. Co-decorated carboxyl, hydroxyl and amine groups on the CQDs surfaces make them as promising polyelectrolyte for gene delivery. Toxicity assay showed a survival rate of 70% under different incubation times and up to 500 μg/mL. The highly water-soluble, stable fluorescence and low toxic CQDs increased the gene expression of DNA plasmid in E. coli bacteria 4-fold more than the control group.Graphical abstractThe overall scheme for gene delivery by CQDs particles.Graphical abstract for this article
  • Oral delivery of paclitaxel by polymeric micelles: A comparison of
           different block length on uptake, permeability and oral bioavailability
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Lai Pan Sze, Ho Yin Li, Ka Lun Alan Lai, Shing Fung Chow, Qingqing Li, Kin Wah KennethTo, Tai Ning Teddy Lam, Wai Yip Thomas Lee Drug solubility and permeability are two major challenges affecting oral delivery, the most popular route of drug administration. Polymeric micelles is an emerging technology for overcoming the current oral drug delivery hurdles. Previous study primarily focused on developing new polymers or new micellar systems and a systematic investigation of the impact of the polymer block length on solubility and permeability enhancement; and their subsequent effect on oral bioavailability is lacking.Herein, by using paclitaxel, a poorly soluble P-glycoproteins (P-gp) substrate, as a model, we aim to assess and compare the drug-loaded micelles prepared with two different molecular weight of poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-b-PCL), with the ultimate goal of establishing a strong scientific rationale for proper design of formulations for oral drug delivery.PEG-b-PCL (750:570) (PEG17-b-PCL5) and PEG-b-PCL (5k:10k) (PEG114-b-PCL88) effectively enhanced the solubility of paclitaxel compared to the free drug. PEG-b-PCL (750:570) increased both P-gp and non P-gp substrate cellular uptake and increased the apparent permeability coefficient of a P-gp substrate.In vivo animal study showed that PEG-b-PCL micelles efficiently enhanced the oral bioavailability of paclitaxel. In addition to solubility enhancement, polymer choice also plays a pivotal role in determining the oral bioavailability improvement, probably via permeation enhancement.In conclusion, the knowledge gained in this study enables rational design of polymeric micelles to overcome the current challenges of oral drug delivery and it also provides a basis for future clinical translation of the technology.Graphical abstractGraphical abstract for this article
  • Investigating the synergetic dispersing effect of hydrolyzed
           biomacromolecule Cellulase and SDS on CuPc pigment
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Yuetai Zhao, Rui Wang, Kuanjun Fang, Yeqiang Tan, Shiquan Chen, Yan Guan, Longyun Hao In this paper, the dispersion performance of biomacromolecule hydrolyzed cellulase from Trichoderma reesei on copper phthalocyanine (CuPc) pigment was first studied. The effect of hydrolysis time, cellulase concentration and environmental pH on the dispersion performance was investigated by particle size distribution and suspension transmittance measurement. The hydrolysis degree of cellulase was determined by FTIR, XRD, UV–vis and fluorescence spectra, potential and particle size analysis, respectively. Subsequently, the hydrolyzed cellulase was combined with sodium dodecyl sulfate (SDS) for acquiring better CuPc suspension based on their synergetic effects on dispersion. The optimal mass ratio of hydrolyzed cellulase to SDS was found to be 1:9. The resulting CuPc dispersion by this hydrolyzed cellulase/SDS composite was characterized by FTIR, TG, TEM, XRD analysis, respectively. This study demonstrated that there were strong interactions between hydrolyzed cellulase and SDS to result in synergistic dispersing effect on CuPc for better stability.
  • A comparative and systematic approach to desolvation and self-assembly
           methods for synthesis of piperine-loaded human serum albumin nanoparticles
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Hossein Abolhassani, Seyed Abbas Shojaosadati The present work aimed to accomplish a comparative and principled study on desolvation and self-assembly methods for synthesis of piperine-loaded human serum albumin nanoparticles (PIP-HSA-NPs). Among drugs, PIP was selected as the hydrophobic model drug. The response surface methodology (RSM)-central composite design (CCD) was employed to precisely study the processes and the interactions between the factors affecting the methods. Optimization was performed to obtain the best formulations for both procedures. Both optimized PIP-HSA-NPs prepared by the two methods were stable and semi-spherical with the size less than 200 nm. The self-assembled PIP-HSA-NPs which were prepared under the optimized conditions with drug encapsulation efficiency (DEE) of 76.8% ± 0.44%, and drug loading efficiency (DLE) of 8.92% ± 0.3% had significantly higher DEE and DLE than the optimized particles obtained from the desolvation method with DEE of 34.1% ± 0.32% and DLE of 1.68 ± 0.11%. The secondary structure of HSA did not change much in self-assembled PIP-HSA-NPs compared to desolvated PIP-HSA-NPs. The self-assembled PIP-HSA-NPs showed more cumulative drug release than desolvated NPs, causing them to have more cytotoxicity on MCF-7 cells at high concentrations. These findings introduce the self-assembly technique as the better chemical method to produce a practical cost-effective carrier for many hydrophobic drugs.Graphical abstractGraphical abstract for this article
  • Biomimetic phosphorylcholine strategy to improve the hemocompatibility of
           pH-responsive micelles containing tertiary amino groups
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Hu Lin, Quan Wang, Rui Zhong, Zhen Li, Weifeng Zhao, Yuanwei Chen, Meng Tian, Xianglin Luo pH-responsive nanocarriers such as polymeric micelles that self-assemble from amphiphilic copolymers containing amino groups have been limited by their significant effects on the blood and thus compromise of their hemocompatibility due to the amino group-induced positive charges. Here we report a biomimetic phosphorylcholine strategy to improve the hemocompatibility of the pH-responsive micelles with positive charges. Amphiphilic copolymers containing different number of tertiary amino groups were synthesized in five steps through ring opening polymerization, azide reaction, thio-bromo “Click” chemistry, and atom transfer radical polymerization to self-assemble biomimetic phosphorylcholine micelles with pH-responsiveness, which shown no significant effects on red blood cells, coagulation, and platelet activation. Moreover, albumin adsorption on the micelles was significantly lower than that of polycaprolactone-methoxypolyethylene glycol (PCL-mPEG) control, and in terms of immune cells, the micelles showed controllable phagocytosis that dependent on the number of tertiary amino groups, in which the one containing four tertiary amino groups in its corresponding copolymer remains had a lower phagocytosis by whole blood leukocyte than that of PCL-mPEG. Based on these results, the hemocompatibility related mechanism of the micelles was discussed and proposed. Our findings demonstrated that this biomimetic phosphorylcholine is a promising strategy to improve the hemocompatibility of the positively charged nanocarriers.Graphical abstractGraphical abstract for this article
  • Interactions of a short hyaluronan chain with a phospholipid membrane
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Piotr Bełdowski, Steven Yuvan, Andra Dėdinaitė, Per M. Claesson, Thorsten Pöschel Hyaluronic acid and phospholipids are two components that are present in the synovial fluid, and both are implicated as important facilitators of joint lubrication. In this work we aim to clarify how hyaluronic acid interacts with a phospholipid bilayer through their molecular interactions at the bilayer surface. To this end we performed molecular dynamics simulations of one hyaluronic acid molecule at a phospholipid bilayer in aqueous solution. The simulations were carried out for two aqueous solutions of equal concentrations, containing either NaCl or CaCl2. We analyzed hydrogen bonds, hydrophobic contacts and cation mediated bridges to clarify how hyaluoronic acid binds to a phospholipid bilayer. The analysis shows that calcium ions promote longer lasting bonds between the species as they create calcium ion bridges between the carboxylate group of hyaluronic acid and the phosphate group of the phospholipid. This type of additional bonding does not significantly influence the total number of contact created, but rather stabilizes the contact. The presented results can facilitate understanding of the role of hyaluronic acid and phospholipid interactions in terms of lubrication of articular cartilage.Graphical abstractGraphical abstract for this article
  • Incorporation of bioflavonoids from Bidens tripartite into micelles of
           non-ionic surfactants – experimental and theoretical studies
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Paweł Śliwa, Karolina Śliwa, Elżbieta Sikora, Jan Ogonowski, Jan Oszmiański, Paulina Nowicka Classical extraction methods used for an isolation of active substances from the plant material are expensive, complicated and often environmentally unfriendly. The ultrasonic assistance micelle-mediated extraction method (UAMME) seems to be an interesting alternative. The aim of this work was to compare an efficiency of water solutions of three non-ionic surfactants: C9-11 Pareth-5, PPG-6 Steareth-7 and PPG-4 Laureth-5 in UAMME of Bidens tripartita. Subsequently, the obtained extracts were separated into two immiscible phases, a polyphenols rich surfactants phase and an aqueous phase by its heating above surfactants cloud points (CPC) or by salting out with NaCl. Along with decreasing the Hydrophilic/Lipophilic Balance (HLB) factor value of surfactants, i.e. increase of the hydrophobicity, a significant decreasing of the flavonoid content was observed. While polyphenols content and antioxidant activity increased. The good surface properties of all surfactants correspond to the high content of phenolic compounds in extracts and both concentration methods resulted in even a 50-fold increase of polyphenols content. Dynamic light scattering measurements (DLS) provided that solubilization of polyphenols, i.e. their incorporation into surfactants’ micelles, occurred with significant enlarging of particle size. Based on the molecular dynamic simulations, the mechanism of polyphenols incorporation into micelles was discussed.Graphical abstractGraphical abstract for this article
  • Non-proteinogenic Amino Acid based Supramolecular Hydrogel Material for
           Enhanced Cell Proliferation
    • Abstract: Publication date: Available online 16 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jaini Flora Arokianathan, Koduvayur A Ramya, Asuma Janeena, Abhijit P. Deshpande, Niraikulam Ayyadurai, Ambrose Leemarose, Ganesh Shanmugam Supramolecular gel material built from low-molecular-weight (LMW) gelators finds potential applications in various fields, especially in drug delivery, cell encapsulation and delivery, and tissue engineering. The majority of the LMW gelators in these applications are based on functionalized peptides/amino acids consisting of proteinogenic amino acids which are proteolytically unstable. Herein, we have developed a new LMW gelator containing non-proteinogenic amino acid namely 2,3-diaminopropionic acid (Dap), a key precursor in the synthesis of many antibiotics namely viomycin and capreomycin, by functionalizing with fluorenylmethoxycarbonyl at both amino terminals of Dap [Fm-Dap(Fm)]. Hydrogelation test at different pH indicates that Fm-Dap(Fm) can form a hydrogel in a wide range of pH (4.9 to 9.1) with minimum hydrogelation concentration depends on the pH. The mechanical strength and thermal stability of the Fm-Dap(Fm) hydrogel material are found to decrease with increasing pH (acidic > neural/physiological > basic). The thermal stability of Fm-Dap(Fm) hydrogels is pH-dependent and elicits high stability at acidic pH. Also, Fm-Dap(Fm) hydrogels exhibit strong thixotropic property where regelation (self-healing) occurs upon release of stress. Morphological analysis indicates the formation of fibrils, which are entangled to form three dimensional network structures. Several spectroscopic measurements provided evidence for the self-assembly of Fm-Dap(Fm) molecules through intermolecular aromatic π-π stacking and hydrogen bonding interactions during hydrogelation. Interestingly, Fm-Dap(Fm) not only exhibits hydrogel formation but also shows cell viability and enhanced cell proliferation at physiological pH (7.4). Further, Fm-Dap(Fm) forms a hydrogel upon co-incubation with vitamin B12 and also exhibits release of vitamin B12 over a period. The current study thus demonstrates the development of a new hydrogel material, based on LMW gelator containing the non-proteinogenic amino acid, which can elicit cell viability, enhanced cell proliferation, drug encapsulation, and drug release properties. Hence, Fm-Dap(Fm) hydrogel could be an ideal material for biomedical applications, especially in tissue engineering and drug delivery.Graphical abstractGraphical abstract for this article
  • PEGylated MoS2 quantum dots for traceable and pH-responsive
           chemotherapeutic drug delivery
    • Abstract: Publication date: Available online 16 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Li Liu, Hongli Jiang, Jian Dong, Wenxian Zhang, Guangyao Dang, Mingfeng Yang, Yanyan Li, Hongyu Chen, Haiwei Ji, Lifeng Dong Since low pH value is widely observed in most of solid tumors, pH-responsive drug delivery system (DDS) can provide a general strategy for tumor-targeting therapy. In this work, a traceable and pH-responsive DDS (MoS2-PEG-DOX) based on MoS2 quantum dots (MoS2 QDs) is successfully developed by covalently grafting MoS2 QDs with diamine-terminated oligomeric polyethylene glycol (PEG) and then loading with a fluorescent antineoplastic anthracycline drug, doxorubicin (DOX). The functionalization of MoS2 QDs with PEG imparts the nanocomposite with strong blue photoluminescence, low cytotoxicity, and excellent physiological stability. The MoS2-PEG-DOX nano-assembly can be effectively taken up by U251 cells, and an accelerated DOX release is then triggered by intracellular acid condition, which in turn diminishing unwanted side effects derived by the incorporation of DOX into healthy cells. Meanwhile, the cellular uptake of the MoS2-PEG-DOX nano-assembly, consequent DOX release and the localization of nanocarrier can be real-time monitored due to the inherent stable fluorescence of MoS2-PEG and DOX. These findings demonstrate that MoS2-PEG-DOX will be promising for high treatment efficacy with minimal side effects in future therapy.Graphical Graphical abstract for this article
  • Covalent grafting of titanium with a cathelicidin peptide produces an
           osteoblast compatible surface with antistaphylococcal activity
    • Abstract: Publication date: Available online 16 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Gerard Boix-Lemonche, Jordi Guillem-Marti, Francesca D’Este, José María Manero, Barbara Skerlavaj Bacterial infection of orthopaedic implants, often caused by Staphylococcus species, may ultimately lead to implant failure. The development of infection-resistant, osteoblast-compatible biomaterials could represent an effective strategy to prevent bacterial colonization of implants, reducing the need for antibiotics.In this study, the widely used biomaterial titanium was functionalized with BMAP27(1-18), an α-helical cathelicidin antimicrobial peptide that retains potent staphylocidal activity when immobilized on agarose beads. A derivative bearing a short spacer with a free thiol at the N-terminus was coupled to silanized titanium disks via thiol-maleimide chemistry. Tethering was successful, as assessed by Contact angle, Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), and X-ray Photoelectron Spectroscopy (XPS), with an average surface mass density of 456 ng/cm2 and a layer thickness of 3 nm. The functionalized titanium displayed antimicrobial properties against a reference strain of Staphylococcus epidermidis with well-known biofilm forming capability. Reduction of bacterial counts and morphological alterations of adhering bacteria, upon 2 h incubation, indicate a rapid contact-killing effect. The immobilized peptide was not toxic to osteoblasts, which adhered and spread better on functionalized titanium when co-cultured with bacteria, compared to non-coated surfaces. Results suggest that functionalization of titanium with BMAP27(1-18) could be promising for prevention of bacterial colonization in bone graft applications.Graphical abstractGraphical abstract for this article
  • C-12 vs C-3 substituted bile salts: an example of the effects of
           substituent position and orientation on the self-assembly of steroid
           surfactant isomers
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jacopo Cautela, Emilia Severoni, Carlos Redondo-Gómez, Maria Chiara di Gregorio, Alessandra Del Giudice, Simona Sennato, Roberta Angelini, Marco D’Abramo, Karin Schillén, Luciano Galantini Biomolecule derivatives are transversally used in nanotechnology. Deciphering their aggregation behavior is a crucial issue for the rational design of functional materials. To this end, it is necessary to build libraries of selectively functionalized analogues and infer general rules. In this work we enrich the highly applicative oriented collection of steroid derivatives, by reporting a rare example of C-12 selectively modified bile salt. While nature often exploits such position to encode functions, it is unusual and not trivial to prepare similar analogues in the laboratory. The introduction of a tert-butyl phenyl residue at C-12 provided a molecule with a self-assembly that remarkably switched from rigid pole-like structures to twisted ribbons at a critical biologically relevant temperature (∼25 °C). The system was characterized by microscopy and spectroscopy techniques and compared with the C-3 functionalized analogue. The twisted ribbons generate samples with a gel texture and a viscoelastic response. The parallel analysis of the two systems suggested that the observed thermoresponsive self-assemblies occur at similar critical temperatures and is probably dictated by the nature of the substituent, but involve aggregates with different structures depending on position and orientation of the substituent. This study highlights the self-assembly properties of two appealing thermoresponsive systems. Moreover, it adds fundamental insights hereto missing in the investigations of the relation between self-assembly and structure of synthetic steroids, which are valuable for the rational design of steroidal amphiphiles.Graphical abstractGraphical abstract for this article
  • Effect of Al3+ ions substitution in novel zinc phosphate glasses on
           formation of HAp layer for bone graft applications
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): M. Mohan Babu, P. Venkateswara Rao, N. Veeraiah, P. Syam Prasad Aluminium doped phosphate based bioglasses have potential applications in the field of bone tissue engineering because of their excellent bioactivity and biocompatibility along with high mechanical strength and controlled dissolution. In the present study, 8ZnO–22Na2O–(24-x)CaO–46P2O5–xAl2O3 (where x = 0, 2, 4, 6, 8 and 10 mol%) glass system was synthesized and investigated by means of XRD, FTIR, SEM and EDS before and after immersion in SBF for 3, 7, 14 and 21days, the physic-chemical properties of samples, including density and microhardness, evaluation of pH and weight loss of glasses in physiological fluid and cell cultural studies like cell viability, cytocompatability and cell proliferation by seeding rMSCs cells on the glass samples in order to throw some light on their structural properties. The results showed that, the density and Vickers hardness found to be increased with theincrease in content of alumina due to the slight increase in the number of octahedrally coordinated Al3+ ions and stronger ionic cross linkages due to insertion of Al3+ ions between phosphate networks. The initial rise in pH and controlled solubility in SBF strongly supports the apatite layer development. The growth of the rMSCs cells on all samples showing good cytocompatability and proliferation up to 6 mol% Al2O3 after that decreases slightly with an increase in alumina content due to network forming action of Al3+ ions in zinc phosphate based glasses. The results confirmed the suitability of these glasses for clinical trials towards bone repair and regeneration resorbable implants.Graphical Graphical abstract for this article
  • Encapsulation of an Endostatin Peptide in Liposomes: Stability, Release,
           and Cytotoxicity Study
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nastaran Rezaei, Faramarz Mehrnejad, Zahra Vaezi, Mosslim Sedghi, S. Mohsen Asghari, Hossein Naderi-Manesh The endostatin protein is a potent inhibitor of angiogenesis and tumor growth. The anti-angiogenic and antitumor properties of full-length endostatin can be mimicked by its N-terminal segment, including residues 1-27. Therefore, our previous studies have shown that a mutant N-terminal peptide which the Zn-binding loop was replaced by a disulfide loop (referred to as the ES-SS peptide) has preserved antiangiogenic and antitumor properties compared to the native peptide. To increase stability and plasma half-life of the ES-SS peptide, the nano-sized liposomal formulations of the peptide with different ratio of phosphocholine (PC) were synthesized. The liposomal peptide formulations possessed an average size of around 100 nm with (−4 to −36 mv) in zeta potential. The encapsulation efficiency of the ES-SS peptide was in the range of 24–54% with different lipid: peptide molar ratios. In vitro release of the peptide from liposomes indicated a complete peptide release after 7 days. Cytotoxicity assay was evaluated using the human umbilical vein endothelial cells (HUVECs) for various concentrations of the liposomal peptide. The results depicted the gradual release of the peptide through liposomes. By comparing with the free peptide, the liposomal peptide formulations have indicated higher cell viability with IC50 value about 0.1 µM. The peptide-liposome interactions, as well as the peptide effect on the liposome structure, were also investigated through coarse-grained molecular dynamics (CG-MD) simulation. The results revealed that the peptides were assembled in the hydrophilic core of the liposome. The peptide behavior in liposome can stabilize the liposome structure and be a response to the observed low peptide release rate. The investigation is promising for designing a liposome-based anti-angiogenesis peptide delivery system.Graphical abstractGraphical abstract for this articleEncapsulation of ES-SS peptide in nano-sized liposome to increase stability and plasma half-life of the endostatin peptide.
  • Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles:
           Proof of concept of alternative probe in colorimetric glucose detection
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Qiqian Liu, Fatima Aouidat, Pasqule Sacco, Eleonora Marsich, Nadia Djaker, Jolanda Spadavecchia Galectins (Gal) are a family of dimeric lectins, composed by two galactoside-binding sites implicated in the regulation of cancer progression and immune responses. In this study, we report for the first time the synthesis and the physical-chemical characterization of galectin-1-complex-gold COOH-terminated polyethlenglicole (PEG)-coated NPs (Gal-1 IN PEG-AuNPs) and their ability to recognize glucose in an aqueous solution with a concentration varying from 10 mM to 100 pM. The chemical protocol consistsof three steps: (i) complexation between galectin-1Gal-1 and tetrachloroauric acid (HAuCl4) to form gold-protein grains; (ii) staking process of COOH-terminated polyethlenglicole molecules (PEG) onto Gal-1-Au complex and (iii) reduction of hybrid metal ions to obtain a colloidal stable solution. During the complexation, the spectral signatures related to the Gal-1 orientation on the gold surface have been found to change due to its protonation state. The effective glucose monitoring was detected by UV-Vis, Raman spectroscopy and Transmission Electron Microscopy (TEM). Overall, we observed that the interaction is strongly dependent on the Gal-1 conformation at the surface of gold nanoparticles.Graphical abstractGraphical abstract for this article
  • Functionalized BaTiO3 enhances piezoelectric effect towards cell response
           of bone scaffold
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Cijun Shuai, Guofeng Liu, Youwen Yang, Wenjing Yang, Chongxian He, Guoyong Wang, Zheng Liu, Fangwei Qi, Shuping Peng Piezoelectric effect of polyvinylidene fluoride (PVDF) plays a crucial role in restoring the endogenous electrical microenvironment of bone tissue, whereas more β phase in PVDF leads to higher piezoelectric performance. Nanoparticles can induce the nucleation of the β phase. However, they are prone to aggregate in PVDF matrix, resulting in weakened nucleation ability of β phase. In this work, the hydroxylated BaTiO3 nanoparticles were functionalized with polydopamine to promote their dispersion in PVDF scaffolds fabricated via selective laser sintering. On one hand, the catechol groups of polydopamine could form hydrogen bonding with the hydroxyl groups of the BaTiO3. On the other hand, the amino groups of polydopamine were able to bond with C-F group of PVDF. As a result, the functionalized BaTiO3 nanoparticles homogeneously distributed in PVDF matrix, which significantly increased the β phase fraction from 46% to 59% with an enhanced output voltage by 356%. Cell testing confirmed the enhanced surface electric cues significantly promoted cell adhesion, proliferation and differentiation. Furthermore, the scaffolds exhibited enhanced tensile strength and modulus, which was ascribed to the rigid particle strengthening effect and the improved interfacial adhesion. This study suggested that the piezoelectric scaffolds shown a potential application in bone repair.Graphical Graphical abstract for this article
  • Enzyme responsive titanium substrates with antibacterial property and
           osteo/angio-genic differentiation potentials
    • Abstract: Publication date: Available online 15 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yonglin Yu, Qichun Ran, Xinkun Shen, Hong Zheng, Kaiyong Cai After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substrates via layer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.Graphical Graphical abstract for this article
  • Biocompatibility and hemocompatibility of hydrothermally derived reduced
           graphene oxide using soluble starch as a reducing agent
    • Abstract: Publication date: Available online 14 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Kannan Badri Narayanan, Hyun Duck Kim, Sung Soo Han Graphene-based nanomaterials (GBNs) have many applications as biomedical materials in tissue engineering and regenerative medicine. We report on the preparation of starch-functionalized reduced graphene oxide nanosheets (SRGO) using soluble starch as a reducing agent in a hydrothermal method, and their in vitro interactions with human skin fibroblasts and red blood cells (RBCs). Our results indicate that soluble SRGO nanosheets were prepared using graphene oxide (GO) as a raw material. SRGO-1 and -10, which were prepared using different concentrations of soluble starch after hydrothermal treatment, were characterized by ultraviolet-visible spectroscopy and showed a peak shift at 260 nm corresponding to the deoxygenation of GO and restoration of the conjugated aromatic structure. Dynamic light scattering and zeta potential measurements were used to determine Z-average sizes and surface charges of GO and SRGOs. X-ray diffractometry, attenuated total reflectance Fourier-transform infrared spectroscopy, and Raman spectroscopy revealed the progressive elimination of labile oxygen functional groups during hydrothermal treatment and restoration of the π-conjugated network. X-ray photoelectron spectroscopy showed de-oxidation of SRGOs, which had high carbon to oxygen ratios (C/O) as compared with GO. Interactions of SRGO-1 and -10 with skin fibroblasts showed excellent biocompatibility even at a concentration of 200 μg/ml with cell viabilities up to 88% and 90%, respectively, whereas notable cytotoxicity was observed for GO at 20 μg/ml. Similarly, SRGO-1 and -10 did not exhibit toxicity to RBCs compared to GO. Biofilm formation and metabolic activities of biofilm by the bacterium Staphylococcus aureus were also evaluated using a crystal violet and a tetrazolium reduction assay, respectively. The described hydrothermal method used to synthesize SRGO provides a cheap, facile, and environmentally friendly means of producing water-dispersible, biocompatible and hemocompatible reduced GOs for the fabrication of novel GBNs for various biomedical applications.Graphical abstractGraphical abstract for this article
  • The impact of lipases on the rheological behavior of colloidal silica
           nanoparticle stabilized Pickering emulsions for biocatalytical
    • Abstract: Publication date: Available online 14 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anja Heyse, Matthias Kraume, Anja DrewsABSTRACTThe use of Pickering emulsions for biocatalytical applications has recently received increased attention in cases where hydrophobic reactants are involved. For process applications, knowledge of the emulsion’s rheology is crucial for the fluid dynamical design of equipment and selection of operating conditions. Colloidal silica nanoparticle stabilized Pickering emulsions usually exhibit shear-thinning behavior caused by a complex particle-particle network. While this has been observed by many authors, no publication has yet dealt with the rheology of silica nanoparticle stabilized Pickering emulsions containing enzymes. Thus, the aim of this study was to investigate the impact of the commonly used biocatalyst lipase (type and concentration), the dispersed phase volume fraction and the silica particle concentration on the rheological behavior of water-in-oil Pickering emulsions. For this purpose, the impact of the named parameters on the viscosity curves were measured. Lipases reduced the viscosities and transferred the rheological behavior from shear-thinning to Newtonian, which might be due to interactions of the lipase molecules via the formation of intermolecular disulfide bonds, which disturb the hydrogen-bond based silica particle-particle network. However, by increasing the dispersed phase volume fraction or the silica particle concentration the rheological behavior of emulsions became again shear-thinning. This work will help to produce bioactive Pickering emulsions with tailor-made characteristics.Graphical Graphical abstract for this article
  • Wheat germ agglutinin liposomes with surface grafted cyclodextrins as
           bioadhesive dual-drug delivery nanocarriers to treat oral cells
    • Abstract: Publication date: Available online 13 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sashini S. Wijetunge, Jianchuan Wen, Chih-Ko Yeh, Yuyu Sun Topical management of oral infection requires combined use of multiple classes of drugs and frequent dosing due to low drug retention rates. The sustained, co-delivery of drugs with different solubilities to cells using nanoparticle drug delivery systems remains a challenge. Here, we developed wheat germ agglutinin (WGA) conjugated liposomes with surface grafted cyclodextrin (WGA-liposome-CD) as bioadhesive dual-drug nanocarriers. We effectively encapsulated two physiochemically different drugs (ciprofloxacin and betamethasone) and demonstrated sustained co-drug release in saliva over a 24 h period in vitro. As proof of therapeutic utility in oral cells, we infected oral keratinocytes with Aggregatibacter actinomycetemcomitans, a bacterial pathogen responsible for chronic periodontal disease. Drug release, resulting from nanocarrier cell binding, produced a significant increase in oral cell survival and synergistically reduced inflammation. These results suggest that WGA-liposome-CD nanocarriers are novel cyto-adhesive candidates for delivering multiple drugs with sustained therapeutic activity for localized drug delivery to oral cells.Graphical abstractGraphical abstract for this article
  • Development of microemulsion of tamsulosin and dutasteride for benign
           prostatic hyperplasia therapy
    • Abstract: Publication date: Available online 13 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Shayara Lopes Ciríaco, Ivana Pereira Santos Carvalho, José Alves Terceiro Neto, José de Sousa Lima Neto, Daniel Henrique Bento de Oliveira, Ana Paula Gomes Pereira Cunha, Ykro Talvanis Duarte Cavalcante, Dayane Tomaz Casimiro da Silva, José Alexsandro da Silva, Ana Lys Bezerra Barradas Mineiro, Maria Zenaide de Lima Chagas Moreno Fernandes, André Luis Menezes Carvalho Benign prostatic hyperplasia (BPH) is a condition characterized by a benign enlargement of the prostate that interferes with the normal flow of urine. This disease is treated with the oral administration of combination therapy comprising α-blockers (tamsulosin) and 5α-reductase inhibitors (dutasteride). However, these compounds have low bioavailability. Thus, transdermal microemulsions aimed at promoting permeation and efficient targeted drug delivery through the skin are used. The objectives of this study were to obtain microemulsions of the combined doses of dutasteride and tamsulosin and evaluate their anti-hyperplastic activity in vivo. A phase diagram (4:1) was obtained for the choice of microemulsions. The microemulsions were characterized in terms of the droplet size, rheology, pH, conductivity, refractive index, in vitro release profile, and antihyperplastic effect in vivo. A method for the simultaneous quantification of drugs was developed using UV–vis spectroscopy. The microemulsions had an average size less than 116 nm, an acidic pH and low viscosity. The conductivity ranged from 6.18 to 185.2 μS/cm. The in vitro release profile was sustained for 6 h. Microemulsions promoted the reduction in the size of testosterone-dependent organs (prostate and seminal vesicles). Transdermal formulations for the treatment of BPH were obtained as a therapeutic alternative to conventional treatments.Graphical abstractGraphical abstract for this article
  • Peptide-Assembled Hydrogels for pH-Controllable Drug Release
    • Abstract: Publication date: Available online 12 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Wenlong Xu, Yue Hong, Aixin Song, Jingcheng Hao A peptide (FFACD) can form hydrogels in DMSO-H2O mixtures (G1), NaOH (G2) and L-arginine (G3) aqueous solutions, respectively. Methyl thiazolyl tetrazolium (MTT) assays demonstrated that G1 and G3 exhibited excellent biocompatibility against HEK293 epithelial cells while G2 had an obvious cytotoxicity. G1 and G3 were used as drug carriers with the high drug loading capacity (DLC). Both hydrophilic DOX and hydrophobic PTX were completely loaded into G1 without destroying the hydrogel, while G3 could only encapsulate DOX. The strong electrostatic interaction between DOX and FFACD molecules could destroy the microstructure of G3 to produce precipitate with a high DOX DLC of 93%. The cytotoxicity assay of G1/DOX against K562 leukemia cells indicated that G1 did not inhabit the efficacy of DOX. In vitro release experiments demonstrated DOX of G3/DOX precipitates could be released 76% at pH = 6.0 of the tumor cells and only about 13% at pH = 7.4 of normal cells.Graphical abstractGraphical abstract for this article
  • A fluorescent signal ⿿removal⿿ sensor via duplex-specific
           nuclease-aided cleavage for miRNA detection in flow cytometry
    • Abstract: Publication date: Available online 12 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Weipan Peng, Yiwen Huang, Qian Zhao, Guowei Lian, Minghui Chen, Jiafang Piao, Xiaoqun Gong, Jin Chang Considered as the next-generation biomarkers, microRNAs play an important role in the early diagnosis of cancers. Here, we designed a fluorescent signal ⿿removal⿿ sensor for one-step, sensitive and specific detection of multiple microRNAs by flow cytometry (FCM). In this work, single-stranded DNA (ssDNA), working as the interlinkage, immobilized the fluorescent nanosphere (FS) onto the SiO2 microspheres surface to form the SiO2-ssDNA-FS probes. When target miRNAs integrated with SiO2-ssDNA-FS probes, the duplex-specific nuclease (DSN) could cleave the ssDNA selectively and release FS with numerous cycles to enhance the fluorescent signal attenuation of SiO2-ssDNA-FS, so as to remarkably improve the analysis sensitivity. It achieved a simple, accurate and quantitative microRNA-21 detection for clinical blood samples. Parallel multi-target detection of microRNA-21 and Let-7d was also realized by different color labeled FS. Moreover, our designed sensor was suitable for other targets⿿ detection with the corresponding probes.Graphic abstractGraphical abstract for this article
  • Silica Nano Supra-assembly for the targeted delivery of therapeutic cargo
           to overcome chemoresistance in cancer
    • Abstract: Publication date: Available online 12 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nanasaheb D. Thorat, Joanna Bauer, Syed A.M. Tofail, Victoria Gascón Pérez, Raghvendra A. Bohara, Hemraj M. Yadav Cancer cells become resistant over the period to chemotherapeutic drugs and pose a challenging impediment for oncologists in providing effective treatment. Nanomedicine allows to overcome chemoresistance and is the focus of our investigation. Silica nanostructures have been highlighted as an interesting drug delivery platform in vitro and in vivo applications. Here we show the validity of nanomedicine approach for targeted chemotherapeutic cargo delivery to overcome chemoresistance in cancer cells both in vitro and in vivo. For demonstrating the concept, we functionalised ˜100 nm long porous silica nanoparticles (˜20 nm diameter ordered pore structure) by conjugating anticancer drug, cytochrome c enzyme and dual-function anticancer aptamer AS1411 in single supra-assembled nanocargos. The supra-assembly on the porous silica nanostructure allows for a high loading of catalytic enzyme cytochrome c, anticancer drug and aptamer. The silica supra-assembly is characterized by transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) surface area analysis. Conjugation of cargoes has been monitored at each step by UV-Vis and Fluorescence spectroscopy. Finally, the constructed supra-assembled nanocarrier tested on chemoresistance colon cancer (HCT116) cells. A pH-responsive, intracellular theranostic cargo delivery has been achieved and the triple action of the nanocargo made an efficient killing of drug resistance colon cancer cells in vitro (˜ 92 % cell death) through triplex therapy effects by supressing the P-glycoprotein (P-gp) level. Furthermore, in vivo animal toxicity studies demonstrated, the supra-assembled nanocargos have encouraging safety index to be used in cancer therapy and drug delivery applications.Graphical abstractGraphical abstract for this article
  • Fabrication of Functional Hollow Magnetic Polymeric Nanoparticles with
           Controllable Magnetic Location
    • Abstract: Publication date: Available online 7 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Waraporn Wichaita, Duangporn Polpanich, Chariya Kaewsaneha, Kulachart Jangpatarapongsa, Pramuan Tangboriboonrat, Kulachart Jangpatarapongsa Hollow magnetic polymeric particles (HoMPs) with controllable location of magnetic nanoparticles and functionality of polymeric double shell were fabricated by means of the facile soft templating method in one-pot. During the in situ miniemulsion polymerization, hexadecane, the Ostwald suppressing agent, acted as a soft template, which later formed a controllable void size. Adjusting ratio and polarity of monomers caused the difference in polymerization kinetics and phase separation, which resulted in HoMPs with two internal architectures, i.e., HoMPs-I with magnetic at the inner shell/void interface and HoMPs-II with magnetic-embedded shell. Both HoMPs-I and II contained high magnetic content (30-50%wt) with sufficient saturation magnetization (16-30 emu/g). With large void volume (>50%) and distinct shell polarity, either hydrophilic Rhodamine B or hydrophobic fluorescein isothiocyanate dye was selectively loaded. After functionalization with a cancer cell target ligand, folic acid (FA), the cellular uptake of HoMPs-FA in HeLa cell was improved with 100% cell viability and without cell destruction. This fabrication method provides a facile mean to tailor surface chemistry and architectures of hollow hybrid particles, which would be potentially used for efficient treatment of cancer tumors.Graphical abstractGraphical abstract for this article
  • Disinfectant-Like Activity of Lipopeptide Biosurfactant Produced by
           Bacillus tequilensis strain SDS21
    • Abstract: Publication date: Available online 3 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anil Kumar Singh, Prakriti Sharma Antiseptics and disinfectants are widely applied for eliminating microorganisms. However, microorganisms dwelling in the biofilm are less susceptible and in some cases resistant to biocide treatment. The present study describes isolation and characterization of lipopeptide biosurfactant exhibiting disinfectant-like activity. Biosurfactant was produced by an endo-rhizospheric bacterium Bacillus tequilensis strain SDS21. Biosurfactant reduced the surface tension of water from 72 to 30 mN/m with CMC of 40 mg/l. The Liquid Chromatography–Mass Spectrometry analysis of biosurfactant suggested it to be a mixture of C14, C15, C16 and C17 surfactin homologues. The lipopeptide biosurfactant exhibited bactericidal activity against planktonic cells and biofilm residing sessile cells. The biosurfactant treatment eradicated more than 99% of bacterial biofilm present on polystyrene, glass and stainless steel surface. The biosurfactant retained its bactericidal and biofilm eradicating activities even after exposure to extreme conditions like high temperate and extreme pH. Unlike some of the commonly used disinfectant, biosurfactant retained its bactericidal and biofilm removing activity even in the hard water containing Mg2+ and Ca2+ ions. Thus, suggesting that biosurfactant produced by strain SDS21 can be used as a disinfectant or in disinfectant-like formulations effective against both planktonic and biofilm residing bacteria.Graphical abstractGraphical abstract for this article
  • High-throughput Sequencing Analysis of Marine Pioneer Surface-biofilm
           Bacteria Communities on Different PDMS-based Coatings
    • Abstract: Publication date: Available online 3 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yuan Sun, Yanhe Lang, ZhengYu Yan, Lei Wang, Zhizhou Zhang Marine biofilms, the attachment of marine microorganisms on artificial surfaces in natural seawater, play critical roles in the development of marine biofouling, which pave ways for the settlement and colonization of sessile invertebrate larvae. Despite the excellent microbe-inhibitory effect of polydimethylsiloxane (PDMS)-based coatings, marine bacteria could still attach to surfaces and form natural biofilms. However, there is little information available on the common structural features of pioneer surface-biofilm bacteria (PSB) communities on different PDMS-based coatings with regard to their compositions, distributions and diversity. Herein, the present study aims to explore the compositional and structural features of the PSB communities on different PDMS-based coatings using 16S rRNA gene amplicon sequencing in terms of the taxonomic structures at phylum, family and genus level. The results revealed the PSB communities on different PDMS-based coatings possessed high similarities in compositional, structural and diversity features, but varied greatly in relative abundance and distributions. Proteobacteria was the most diverse and overwhelming phylum in biofilms formed on all PDMS-based coatings, followed by Cyanobacteria. In addition, the decreased abundance of Proteobacteria and the increased abundance of Cyanobacteria on the carbon nanotubes (CNTs)-modifed PDMS composites (CPCs) may contribute to their differential anti-biofouling effect against the colonization of juvenile macrofoulers.Graphical Graphical abstract for this article
  • Aptamer/Photosensitizer Hybridized Mesoporous MnO2 Based Tumor Cell
    • Abstract: Publication date: Available online 1 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Wei Liu, Kaixiang Zhang, Luyang Zhuang, Junjie Liu, Wu Zeng, Jinjin Shi, Zhenzhong ZhangABSTRACTHerein, we report a turn-on strategy for selectively killing the tumor cell via combining the singlet-oxygen quenching MnO2 and tumor cell-targeting aptamer. The photosensitizers were in the quenching state when loaded in the mesoporous MnO2 (mMnO2) nanoparticles and sealed by the aptamer on the particle surface. The aptamer can selectively recognize the specific membrane protein on the tumor cell and release the photosensitizers, activating the photosensitizer and killing the tumor cells. The specific binding-induced “off-on” switching of singlet oxygen generation reduced the damage to the nearby healthy cells to a large extent. The high loading ability for photosensitizer and the GSH consumption property of mMnO2 endow the system with high local concentration of singlet-oxygen for killing the target tumor cell. The high selectivity and efficiency of the constructed singlet oxygen regulating system will pave a new way for utilizing PDT in cancer precise treatment.Graphical Graphical abstract for this article
  • Study of alkaline phosphatase interaction with putrescine using
           multi-spectroscopic and docking methods
    • Abstract: Publication date: Available online 24 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nasim Babaknejad, Behzad Shareghi, Ali Akbar Saboury The vast application of enzymes in various industries has encouraged scientists to optimize enzymes activity and stability. Solvent engineering is a well-known method used for stabilizing enzymes. Putrescine is a polyamine that can serve as as an enzyme stabilizer. In this study, the structure and activity of bovine alkaline phosphatase (BALP) were studied in the presence of putrescine. The results confirmed that putrescine could bind to BALP and change the UV-Visible spectra of the enzyme. Moreover, putrescine quenched the BALP fluorescence spectra by static quenching mechanism. Putrescine interacted with BALP spontaneously by different forces such as van der Waals and hydrogen bonding. Circular dichroism spectra studies also revealed that the BALP structure was changed in the presence of putrescine. Furthermore, the kinetic parameters showed that BALP was activated by putrescine. Moreover, docking studies confirmed that hydrogen bonds, van der Waals forces and hydrophobic interactions played the major role in the BALP-put complex formation. In general, these results showed that putrescine could modify the BALP activity and structure.Graphical abstractGraphical abstract for this article
  • Meglumine-based supra-amphiphile self-assembled in water as a skin drug
           delivery system: Influence of unfrozen bound water in the system
    • Abstract: Publication date: Available online 23 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ana Luiza R. de Souza, Mariana Fonseca, Leonardo Miziara Barboza Ferreira, Suzy S.S. Kurokawa, Charlene P. Kiill, Natália N. Ferreira, Alexandre dos Santos Pyrrho, Victor H.V. Sarmento, Amélia M. Silva, Maria Palmira D. Gremião, Clóvis A. Ribeiro Hexagonal liquid crystals and supramolecular polymers from meglumine-based supra-amphiphiles were developed as drug delivery systems to be applied on the skin. The influence of fatty acid unsaturation on the structure and mechanical properties was evaluated. Moreover, we have investigated the system biocompatibility and how the type of water could influence its bioadhesive properties. Meglumine-oleic acid (MEG-OA) was arranged as hexagonal liquid crystals at 30–70 wt% water content, probably due to its curvature and increased water solubility. Meglumine-stearic acid (MEG-SA) at 10-80 wt% water content self-assembled as a lamellar polymeric network, which can be explained by the low mobility of MEG-SA in water due to hydrophobic interactions between fatty acid chains and H-bonds between meglumine and water molecules. Both systems have shown suitable mechanical parameters and biocompatibility, making them potential candidates to encapsulate therapeutic molecules for skin delivery. Moreover, a strong positive correlation between the amount of unfrozen bound water in meglumine-based systems and the bioadhesion properties was observed. This work shows that a better understanding of the physicochemical properties of a drug delivery system is extremely important for the correlation with the desired biological response and, thus, improve the product performance for biomedical applications.Graphical abstractGraphical abstract for this article
  • Interaction of cysteine and its derivatives with monolayers of
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): J.M. Arias, S.B. Díaz, A. Ben Altabef, F.G. Dupuy Molecular interactions between l-cysteine (Cys) and its ester derivatives (Cysx); l-cysteine ethyl ester (CE), l-cysteine methyl ester (CM) and N-acetyl l-cysteine (NAC) with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers were investigated using Langmuir film balance technique. The effect of charge on monolayers made of cysteine and three ester derivatives with DPPC was investigated by working with un-buffered and buffered subphases. Also, the effects of cysteine derivatives interaction with DPPC monolayers were studied measuring the change in the surface tension upon aminoacid injection in the subphase whilst keeping lipid molecular density and lateral packing controlled.Cysteine and its ester derivatives showed interfacial activity reducing the air/water surface tension (πi) by 4 mN m−1. However, ester derivatives were able to insert into preformed DPPC monolayers at much higher surface pressures (Δπ), indicating a preferential interaction of Cysx with DPPC.The results indicate that, although the different derivatives of cysteine presented low surface activity, they were able to favourably interact with DPPC monolayers. Also, compression isotherms experiments in binary mixtures indicate that the more surface active compounds stabilized the gel phase of DPPC. The charge on cysteine and its derivatives did not increase the observed effects.Graphical abstractGraphical abstract for this article
  • Multifunctional MoS2 nanosheets with Au NPs grown in situ for synergistic
           chemo-photothermal therapy
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Maosheng Xu, Kunsong Zhang, Yubing Liu, Jie Wang, Ke Wang, Yuanqing Zhang The treatment of cancer has always been a worldwide problem. The combination of chemotherapy and other therapies would be a promising therapy strategy. Photothermal therapy (PTT) does not cause serious side effects and has dual control of time and space, which was considered to be a good method for combination with chemotherapy. Here, we modified multifunctional nanosheets based on in-situ gold nanoparticles (Au NPs) grown molybdenum disulfide (MoS2) to combine their excellent photothermal conversion and drug loading capabilities. More importantly, the target peptide cRGD modified nanosheets have good selectivity to tumor cells rich in integrin receptors, and drug loading and drug release tests showed that it could carry enough chemotherapeutic drugs and had excellent photo controlled release performance. In vitro cell experiments showed that the drug-loaded multifunctional nanosheets had good anti-tumor effect under 808 nm laser irradiation. Therefore, the multifunctional nanosheets designed in this paper have great potential in the study of targeted drug release and chemo-photothermal therapy combined therapy, and are of great significance for in vivo and clinical research.Graphical abstractGraphical abstract for this articleMultifunctional MoS2 nanosheets with Au NPs grown in situ for synergistic chemo-photothermal therapy.
  • Nanoparticles at biointerfaces: Antibacterial activity and nanotoxicology
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Sina Kheiri, Xinyu Liu, Michael Thompson Development of a biomaterial that is resistant to the adhesion and consequential proliferation of bacteria, represents a significant challenge in terms of application of such materials in various aspects of health care. Over recent years a large number of synthetic methods have appeared with the overall goal of the prevention of bacterial adhesion to surfaces. In contrast to these artificial techniques, living organisms over millions of years have developed different systems to prevent the colonization of microorganisms. Recently, these natural approaches, which are based on surface nanotopography, have been mimicked to fabricate a modern antibacterial surface. In this vein, use of nanoparticle (NP) technology has been explored in order to create a suitable antibacterial surface. However, few studies have focused on the toxicity of these techniques and the ecotoxicity of NP materials on mammalian and bacterial cells simultaneously. Researchers have observed that the majority of previous studies have demonstrated some of the extents of the harmful impacts on mammalian cells. Here, we provide a critical review of the NP approach to antibacterial surface treatment, and also summarize the studies of toxic effects caused by metal NPs on bacteria and mammalian cells.Graphical abstractGraphical abstract for this article
  • Self-gelling electroactive hydrogels based on chitosan–aniline
           oligomers/agarose for neural tissue engineering with on-demand drug
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Babak Bagheri, Payam Zarrintaj, Sachin Subhash Surwase, Nafiseh Baheiraei, Mohammad Reza Saeb, Masoud Mozafari, Yeu Chun Kim, O Ok Park Designing biomimetic scaffolds is an intellectual challenge of the realm of regenerative medicine and tissue engineering. An electroactive substrate should meet multidisciplinary mimicking the mechanical, electrical, and electrochemical properties of neural tissues. Hydrogels have been known platforms to regulate neural interface modulus, but the lack of conductivity always hampered their applications; hence, developing conductive hydrogels with on-demand drug release has become a concern of tissue engineering. In this work, electroactive hydrogels based on chitosan–aniline oligomer and agarose with self-gelling properties were synthesized, and their electrical, thermal, and electrochemical properties were characterized by Fourier transform infrared (FTIR), cyclic voltammetry (CV), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA), and four probe method . The conductivity of the as-prepared aniline oligomer-based hydrogel was ∼10−4 S/cm; which fell within the range of conductivities appropriate for applications in tissue engineering. The aniline oligomer played a key role in controlling the hydrogel properties by regulating the glass transition temperature and thermal properties. In addition, the swelling and degradation rates were decreased because of the hydrophobic properties of the aniline oligomer. The swelling capacity of the pristine hydrogel was ∼800%, while that of the conductive hydrogel decreased to ∼300%. The conductivity of the hydrogel was regulated by modifying the macromolecular architecture through aniline oligomer incorporation thanks to its conductivity on-demand drug release was observed by electrical stimulation, in which a large amount of the drug was released by voltage application. Biocompatibility analysis of the designed hydrogel was indicative of the conductivity enhancement, as reflected in the growth and proliferation of cellular activity.Graphical abstractGraphical abstract for this article
  • Nucleic acid carrier composed of a branched fatty acid lysine
           conjugate—Interaction studies with blood components
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Julia Giselbrecht, Sophia Wiedemann, Shashank Reddy Pinnapireddy, Nathalie Goergen, Harald Loppnow, Daniel Sedding, Frank Erdmann, Udo Bakowsky, Gerd Hause, Marlene Lúcio, Andreas Langner, Christian Wölk For the development of gene therapeutics for systemic administration several hurdles have to be overcome. In this article we screen the branched fatty acid lysine conjugate T14diLys, a newly designed cationic lipid for lipofection, regarding this problem. The structure and particle size of lipoplexes, prepared with lipid formulations which are based on these lipid as nucleic acid complexing agent, are investigated in absence and presence of serum. Nuclease digestion assays were performed to evaluate the protective characteristics of the lipid formulation for the complexed nucleic acid. Furthermore, the lipid formulation is investigated regarding the interaction with different serum proteins to get first insights into the protein corona formation. Another focus is set on the hemocompatibility using in vitro assays for hemolysis and complement activation and the irritation test at the chorion allantois membrane of the chicken embryo as in vivo model. Finally, preliminary transfection efficiency studies with cell culture models for cells which are assessable via systemic administration are performed to evaluate possibilities for future therapeutic applications of the new lipid formulations. Summarizing, T14diLys with the co-lipid DOPE can be used to prepare a lipoplex formulation which can be applied systemically and can be used to develop gene therapeutics for targeting endothelial cells, macrophages, or leucocytes.Graphical abstractGraphical abstract for this article
  • CD47-targeted bismuth selenide nanoparticles actualize improved
           photothermal therapy by increasing macrophage phagocytosis of cancer cells
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Zhaoming Guo, Ye Liu, Hao Zhou, Kun Zheng, Dong Wang, Mingli Jia, Pengcheng Xu, Kun Ma, Changhao Cui, Li Wang CD47, a transmembrane protein overexpressed in most tumors, limits macrophage phagocytosis by interacting with macrophage signal-regulated protein α (SIRPα). In this study, we have developed CD47-targeted bismuth selenide nanoparticles (Ab-PEG-Bi2Se3) that increase phagocytosis of cancer cells by macrophages to actualize improved photothermal therapy (PTT). The functionalized nanoparticles were constructed by conjugating anti-CD47 antibody (Ab) to PEGylated bismuth selenide nanoparticles (PEG-Bi2Se3). The anti-CD47 antibody modified on the nanoparticles enhanced the phagocytic activity of macrophages toward tumor cells by specifically blocking the crosstalk between CD47 and SIRPα. Meanwhile, Ab-PEG-Bi2Se3 showed excellent photothermal performance including strong near infrared (NIR) absorbance, high photothermal conversion efficiency and photostability, and exhibited outstanding in vitro PTT effect under NIR laser irradiation. In vivo therapeutic experiments revealed that this CD47-targeted PTT nanoagent, with the assistance of enhanced macrophage phagocytosis, achieved the goal of tumor eradication. Besides, toxicity studies confirmed that Ab-PEG-Bi2Se3 had good biocompatibility. In conclusion, Ab-PEG-Bi2Se3 may serve as an efficient PTT platform in combination with macrophage-mediated immunotherapy to improve antitumor efficacy.Graphical abstractGraphical abstract for this article
  • Evidence of oxygen defects mediated enhanced photocatalytic and
           antibacterial performance of ZnO nanorods
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Jaspal Singh, Subhavna Juneja, Shatrudhan Palsaniya, Ashis. K. Manna, R.K. Soni, Jaydeep Bhattacharya Defect engineered one-dimensional (1D) ZnO nanostructures have found great interest in diverse fields, including water detoxification and environmental remediation. In this article, we report a facile, low-temperature hydrothermal synthesis of defect enriched ZnO nanorods at different pH conditions. The dimension of all the synthesized ZnO nanostructures was restricted to 1D with changes only in their aspect ratios, unlike previous reports where change in morphology accompanies the effect of pH. With an increment in the pH value of the reaction mixture, oxygen defect concentration was controlled and confirmed using XPS and Raman spectroscopy. Considerable increase in optical light absorption and reduction in the bandgap, as inferred from the UV–vis study, corroborating the pH-dependent enrichment of defect states in 1D ZnO. Superior photosensitivity of oxygen defect rich ZnO nanorods was utilized to study their sunlight-induced photocatalytic and bactericidal activity towards its application in wastewater treatment. Within 4 h and 30 min of sunlight exposure (900 W/cm2), a 100% bacterial population (S.aureus, 106 cells/m) killing and complete degradation of methylene blue dye (10μM) were achieved. Enhanced reactive oxidative species (ROS) formation due to the presence of additional oxygen defect states is ascribed to be the prime factor facilitating improved degradation efficiency. Additionally, during the optimization study, ZnO nanorods were found to be active against bacterial cells even in the absence of light opening avenues in antimicrobial food packaging and protective surface coatings.Graphical abstractGraphical abstract for this article
  • Griseofulvin solvate solid dispersions with synergistic effect against
           fungal biofilms
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Hisham Al-Obaidi, Radoslaw M. Kowalczyk, Rachith Kalgudi, Mohammed Gulrez Zariwala Fungal biofilms are invariably recalcitrant to antifungal drugs and thus can cause recurrent serious infections. The aim of this work was to prepare highly effective form of the antifungal drug griseofulvin using the chloroform solvate embedded into different polymeric matrices. Based on their solid solubility, solvated (chloroform) and non-solvated (methanol and acetone) solid dispersions were prepared using different materials: silica, microcrystalline cellulose, polyvinylpyrrolidone and hydroxypropyl methylcellulose acetate succinate (HPMCAS) by which HPMCAS dispersions showed the highest solubility of about 200 μg/mL compared with ∼30 μg/mL for pure griseofulvin. The anti fungal potential of griseofulvin was assessed against the dermatophytes T. rubrum. Metabolic and protease activity of T. rubrum NCPF 935 with and without the presence of GF:HPMCAS chloroform solvates showed significant reduction compared to the untreated control after 24 h period. Confocal laser scanning microscopy showed thin hyphae compared to Control and GF:HPMCAS (non solvated). Dynamic vapour sorption data showed that HPMCAS formed most stable solvate structure preventing recrystallization and solvate expulsion, which could explain the disruptive effect of the biofilms. This could be explained by the formed hydrogen bonds as revealed by the solid and liquid state NMR data, which was further confirmed via thermal and FTIR analyses.Graphical abstractGraphical abstract for this article
  • Enhanced biolubrication on biomedical devices using hyaluronic acid-silica
           nanohybrid hydrogels
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Changha Hwang, YouJin Min, Yun-Jeong Seong, Dae-Eun Kim, Hyoun-Ee Kim, Seol-Ha Jeong In this work, highly lubricous hyaluronic acid-silica (HA-SiO2) nanohybrid coatings were fabricated through a sequential process consisting of a sol-gel followed by electrophoretic deposition (EPD). SiO2 nanoparticles were uniformly distributed in the coating layers, and the coating thickness was identified as approximately 1–2 μm regardless of the amount of SiO2. Incorporation of SiO2 into the HA polymer matrix enhanced the mechanical stability of the nanohybrid coatings, indicating greater interfacial bonding strength compared to HA coating layers alone. In addition, due to improved stability, the nanohybrid coatings showed excellent biolubrication properties, which were evaluated with a tribological experiment. These results indicate that the nanohybrid coatings have great potential to be used in biomedical applications that require superior biolubrication properties.Graphical abstractGraphical abstract for this article
  • Biogenic nanoparticles: Synthesis, stability and biocompatibility mediated
           by proteins of Pseudomonas aeruginosa
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Melisa A. Quinteros, José O. Bonilla, Silvana V. Alborés, Liliana B. Villegas, Paulina L. Páez The development of environmental friendly new procedures for the synthesis of metallic nanoparticles is one of the main objectives of nanotechnology. Plants, algae, fungi and bacteria for the production of nanomaterials are viable alternatives due to their low cost, the absence of toxic waste production and their highly energy efficiency. It is also known that biosynthesized silver nanoparticles (AgNPs) show higher biocompatibility compared to the chemically-synthesized ones. In previous results, biosynthesized AgNPs were obtained from the supernatant of Pseudomonas aeruginosa, and they showed a bigger antimicrobial activity against different bacterial species compared to the chemically-synthesized ones. The aim of this work was to analyze the capping of biosynthesized AgNPs using techniques such as transmission electron microscopy (TEM), infrared spectroscopy (IR), and protein identification through mass spectrometry (MS) in order to identify the compounds responsible for their formation, stability and biocompatibility. The TEM images showed that AgNPs were surrounded by an irregular coverage. The IR spectrum showed that this coverage was composed of carbohydrates and/or proteins. Different proteins were identified in the capping associated to biosynthesized AgNPs. Some proteins seem to be important for their formation (Alkyl hydroperoxide reductase and Azurin) and stabilization (Outer membrane protein OprG and Glycine zipper 2 T M domain-containing protein). The proteins identified with the capability to interact with some biomolecules can be responsible for the biocompatibility and may be responsible for the bigger antimicrobial activity than AgNPs have previously shown. These results are pioneers in the identification of proteins in the capping of biosynthesized AgNPs.Graphical abstractGraphical abstract for this article
  • Real-time measurements of milk fat globule membrane modulation during
           simulated intestinal digestion using electron paramagnetic resonance
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Maha Alshehab, Madhu S. Budamagunta, John C. Voss, Nitin Nitin Milk Fat Globules with their unique interfacial structure and membrane composition are a key nutritional source for mammalian infants, however, there is a limited understanding of the dynamics of fat digestion in these structures. Lipid digestion is an interfacial process involving interactions of enzymes and bile salts with the interface of suspended lipid droplets in an aqueous environment. In this study, we have developed an electron paramagnetic resonance spectroscopy approach to evaluate real time dynamics of milk fat globules interfacial structure during simulated intestinal digestion. To measure these dynamics, natural milk fat globule membrane was labeled with EPR-active probe, partitioning of EPR probes into MFGs membrane was validated using saturation-recovery measurements and calculation of the depth parameter Φ. After validation, the selected spin probe was used to evaluate the membrane’s fluidity as a measure of the interface’s modulation in the presence of bile salts and pancreatic lipase. Independently, bile salts were found to have a rigidifying effect on the spin probed MFGM, while pancreatic lipase resulted in an increase in membrane fluidity. When combined, the effect of lipase appears to be diminished in the presence of bile salts. These results indicate the efficacy of EPR in providing an insight into small time scale molecular dynamics of phospholipid interfaces in milk fat globules. Understanding interfacial dynamics of naturally occurring complex structures can significantly aid in understanding the role of interfacial composition and structural complexity in delivery of nutrients during digestion.Graphical abstractGraphical abstract for this article
  • Stimuli-responsive biohybrid nanogels with self-immolative linkers for
           protein protection and traceless release
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Yahui Guo, Yue Zhang, Zhanghao Niu, Yongfang Yang Nanogels have been applied in protein delivery due to the nanoscale sizes and the crosslinked structures. However, the release of protein molecules from the nanogels without damages to the structures and functionalities is quite a challenging research subject. In this research, responsive self-immolative linker dithioethyl carbamate bond is introduced to connect protein and polymer in the nanogel so that traceless release of protein occurs upon addition of glutathione (GSH) or dithiothreitol (DTT). Thermoresponsive polymer poly(di(ethylene glycol) methyl ether methacrylate-co-2-(2-(2-hydroxyethyl) disulfanyl) ethyl methacrylate) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, and was modified with 4-nitrophenyl chloroformate yielding polymer chains with pendant dithioethyl carbonate groups. The dithioethyl carbonate groups were reacted with amine groups of lipases resulting in the formation of dithioethyl carbamate bonds. Meanwhile, biohybrid nanogels were prepared by crosslinking the polymer chains with lipases. The immobilized lipase in the nanogels exhibited enhanced heat and acid resistance. Once the nanogels were treated with GSH or DTT, lipase could be released with no residual groups and most of its bioactivity was recovered.Graphical abstractGraphical abstract for this article
  • Development and characterization of an improved formulation of cholesteryl
           oleate-loaded cationic solid-lipid nanoparticles as an efficient non-viral
           gene delivery system
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): María J. Limeres, Marc Suñé-Pou, Silvia Prieto-Sánchez, Cristina Moreno-Castro, Alejandro D. Nusblat, Cristina Hernández-Munain, Guillermo R. Castro, Carlos Suñé, Josep M. Suñé-Negre, María L. Cuetsas Nanoparticle-mediated plasmid delivery is considered a useful tool to introduce foreign DNA into the cells for the purpose of DNA vaccination and/or gene therapy. Cationic solid-lipid nanoparticles (cSLNs) are considered one of the most promising non-viral vectors for nucleic acid delivery. Based on the idea that the optimization of the components is required to improve transfection efficiency, the present study aimed to formulate and characterize cholesteryl oleate-containing solid-lipid nanoparticles (CO-SLNs) incorporating protamine (P) to condense DNA to produce P:DNA:CO-SLN complexes as non-viral vectors for gene delivery with reduced cytotoxicity and high cellular uptake efficiency. For this purpose, CO-SLNs were used to prepare DNA complexes with and without protamine as DNA condenser and nuclear transfer enhancer. The main physicochemical characteristics, binding capabilities, cytotoxicity and cellular uptake of these novel CO-SLNs were analyzed.Positively charged spherical P:DNA:CO-SLN complexes with a particle size ranging from 330.1 ± 14.8 nm to 347.0 ± 18.5 nm were obtained. Positive results were obtained in the DNase I protection assay with a protective effect of the genetic material and 100% loading efficiency was achieved at a P:DNA:CO-SLN ratio of 2:1:7. Transfection studies in human embryonic kidney (HEK293T) cells showed the versatility of adding protamine to efficiently transfect cells, widening the potential applications of CO-SLN-based vectors, since the incorporation of protamine induced almost a 200-fold increase in the transfection capacity of CO-SLNs without toxicity.These results indicate that CO-SLNs with protamine are a safe and effective platform for non-viral nucleic acid delivery.Graphical abstractGraphical abstract for this article
  • Exploring the mechanism behind improved osteointegration of phosphorylated
           titanium implants with hierarchically structured topography
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Nan Jiang, Zhijun Guo, Dan Sun, Birol Ay, Yubao Li, Yutao Yang, Peijie Tan, Li Zhang, Songsong Zhu Titanium (Ti) and its alloys have been frequently used in dental and orthopedic implants, but the undesired oxide layer easily formed on the surface tends to be the cause of implant failure for Ti-based implants. To address this problem, we herein prepared a phosphorylated Ti coating (TiP-Ti) with a micro/nano hierarchically structured topography on commercially pure Ti implants by a hydrothermal method to improve its osteointegration capacity. The surface morphology, chemical composition, and biological activity were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), contact-angle measurement, and protein adsorption assay. Osteointegration of TiP-Ti implants in rat tibia was investigated by biomechanical testing, micro-CT and histological analyses. We further explored the proposed mechanism which improves osteointegration of TiP-Ti implants by proliferation, adhesion, and differentiation assays of rat bone marrow mesenchymal stem cells (BMSCs). Our results demonstrated that the improved osteointegration mainly benefited from the better spread and adhesion of BMSCs on the micro/nano hierarchically structured TiP-Ti surfaces compared to hydroxyapatite coated Ti (HA-Ti), the positive control, and untreated Ti (untreated-Ti), the negative control. In conclusion, TiP-Ti surface is a promising candidate implant surface design to accelerate the osteointegration of Ti-based implants in biomedical applications.Graphical abstractGraphical abstract for this article
  • Influence of pH on interaction of silver nanoparticles - protein: Analyses
           by spectroscopic and thermodynamic ideology
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): A. Mohammed Siddiq, Devaraj Murugan, Rachana Srivastava, Md. Sayem Alam To investigate the interaction between bovine serum albumin (BSA) and silver nanoparticles (AgNPs) at five different pHs (below (3.0 and 4.0), above (7.4 and 9.2) and at the isoelectric point (4.7) of BSA) by spectroscopic (viz., UV–vis, fluorescence, circular dichroism (CD)), microscopic (viz., atomic force microscopy (AFM), transmission electron microscopy (TEM), field emission scanning electron microscope (FESEM)) and thermodynamic (viz., isothermal titration calorimetry (ITC)) methods. The fluorescence quenching spectra provided binding constants via Stern-Volmer plot, quenching constant (Ksv) and rate constant (Kq) were calculated. From the CD spectra, it is clear that the α-helix decreases by increasing the AgNP’s concentration. However, at isoelectric point (pH = 4.7), BSA shows more helicity in the presence of AgNPs, which indicates that the structures of BSA become more ordered and stable, and aggregation occurs at strong acidic (3.0), and basic medium (9.2) Fluorescence spectra also indicate the aggregation of the protein at strong acidic (pH = 3.0) and basic medium (pH = 9.2). Furthermore, the morphological and topographical evolute ion upon the interaction was examined using TEM, FESEM, and AFM. The studies conclude the effect of the pH in the medium and behavior of AgNPs with BSA by using different spectroscopic and microscopic techniques.Graphical abstractGraphical abstract for this article
  • Fe3O4@OA@Poloxamer nanoparticles lower triglyceride in hepatocytes through
           liposuction effect and nano-enzyme effect
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Yuxiang Sun, Fangfang Shi, Yajun Niu, Yu Zhang, Fei Xiong Lipid deposition induced various diseases including nonalcoholic fatty liver and hyperlipemia. The excessive accumulation of triglyceride (TG) and the deposition of fat were the two most critical causes. Here, we developed Fe3O4@OA@Poloxamer nanoparticles (NPs) with amphiphilic structures, which exhibited an excellent role in eliminating excess TG. Hydrophobic TG was adsorbed efficiently by Fe3O4@OA@Poloxamer NPs through the “liposuction effect” and the formation of NPs@TG complex was then conducted. The NPs@TG complex was further enclosed by the endosome based on the endocytosis and subsequently was taken into the lysosome, degrading with the help of lipases. Meanwhile, the “nano-enzyme effect” of Fe3O4 NPs recovered the lipid-regulated proteins including PPARα, further triggering biodegradation pathways of TG, although the lipid-regulated proteins were obviously inhibited in the high-fat hepatocytes models. These two mechanisms of Fe3O4@OA@Poloxamer NPs together achieved the down-regulation of TG in vivo and in vitro. Therefore, our findings provided a novel thought in treating these diseases associated with lipid deposition, that is, nanoparticles modified by specific structure exhibit a superior TG removal.Graphical abstractGraphical abstract for this article
  • Encapsulation of gadolinium ferrite nanoparticle in generation 4.5
           poly(amidoamine) dendrimer for cancer theranostics applications using low
           frequency alternating magnetic field
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Tefera Worku Mekonnen, Yihenew Simegniew Birhan, Abegaz Tizazu Andrgie, Endiries Yibru Hanurry, Haile Fentahun Darge, Hsiao-Ying Chou, Juin-Yih Lai, Hsieh-Chih Tsai, Jen Ming Yang, Yen-Hsiang Chang Iron oxide-based magnetic resonance imaging (MRI) contrast agents have negative contrast limitations in cancer diagnosis. Gadolinium (Gd)-based contrast agents show toxicity. To overcome these limitations, Gd-doped ferrite (Gd:Fe3O4 (GdIO) nanoparticles (NPs) were synthesized as T1-T2 dual-modal contrast agents for MRI-traced drug delivery. A theranostics GdIO encapsulated in a Generation 4.5 PAMAM dendrimer (G4.5-GdIO) was developed by alkaline coprecipitation. The drug-loading efficiency of the NPs was ∼24%. In the presence of a low-frequency alternating magnetic field (LFAMF), a maximum cumulative doxorubicin (DOX) release of ∼77.47% was achieved in a mildly acidic (pH = 5.0) simulated endosomal microenvironment. Relaxometric measurements indicated superior r1 (5.19 mM−1s−1) and r2 (26.13 mM−1s−1) for G4.5-GdIO relative to commercially available Gd-DTPA. Thus, G4.5-GdIO is promising as an alternative noninvasive MRI-traced cancer drug delivery system.Graphical abstractGraphical abstract for this article
  • Sensitively-selective determination of Propyl Paraben preservative based
           on synergistic effects of polyaniline-zinc-oxide nano-composite
           incorporated into graphite paste electrode
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Manasa G, Ronald J. Mascarenhas, Basavanakote M. Basavaraja The application of Propyl Paraben (PP) as a chemical preservative has expanded and diversified. Widespread use of products containing PP has resulted in its ubiquitous occurrence in environment and biological fluids among the general population. Several in-vivo studies have associated PP with estrogenic activity and malfunctioning of reproductive organs. In this work, we have developed a highly sensitive voltammetric sensor for PP detection based on polyaniline-zinc-oxide nano-composite modified carbon paste electrode (PANI/ZnO/MCPE). The synthesized nano-composite was characterized using spectroscopic techniques. Experimental conditions such as supporting electrolyte, their pH and scan rate were optimized to attain a well defined PP anodic peak current at 690 mV. Our experiments indicate a strong synergistic interaction between ZnO and PANI, resulting in the magnification of PP current with a declined over-potential, compared to bare CPE. We have proposed a mechanism of π-π interaction between PP and PANI/ZnO/MCPE. The electrode process was characterized to be irreversible; diffusion controlled and proceeds with an exchange of 1eˉ and H+. Noteworthy analytical performance over wide linear range from 100.0 to 1.0 μM, with a detection limit of 0.13 μM and anti-interference characteristics were ascertained. A significant advantage of this sensor is its inability to detect micro molar concentrations of ascorbic acid (AA) while PP is detected at micro levels in the presence of high concentrations of AA. The versatility of this sensor was demonstrated by efficaciously applying it to trace analysis of complex real samples such as pharmaceutical formulations, biological samples and lake water with good recoveries.Graphical abstractGraphical abstract for this article
  • Methotrexate and Curcumin co-encapsulated PLGA nanoparticles as a
           potential breast cancer therapeutic system: In vitro and in vivo
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Molood Alsadat Vakilinezhad, Azadeh Amini, Tahereh Dara, Shohreh Alipour Nanoparticulate delivery systems have been noticed for chemotherapeutical delivery due to their ability in controlling the drug release and reducing the side effect. These systems could also be used to deliver two drugs or more simultaneously, inhibiting the development of resistant cancerous cells. Methotrexate (MTX), one of the most frequently used chemotherapeutic agent, and Curcumin (CUR), a natural chemopreventive compound, have shown promising results in treatment or controlling the progression of cancer. The aim of this study is to prepare and evaluate polymeric nanoparticles for co-delivery of MTX and CUR. The PLGA nanoparticles were prepared and characterized in respect of their particles size, morphology, drug encapsulation efficiencies, release patterns, cell cytotoxicity, and in vivo efficacy. Altering MTX and CUR amounts leads to particle size of 142.3 ± 4.07 nm with MTX encapsulation efficiency of 71.32 ± 7.8% and CUR encapsulation efficiency of 85.64 ± 6.3%. These particles showed significantly higher cytotoxicity in comparison with free MTX or CUR or even their solo-loaded formulations. The in vivo results showed the synergic effect of MTX and CUR co-delivery on inhibiting the progression of breast cancer. Considering the appropriate in vitro properties of acquired nanoparticles for controlled drug delivery and the satisfactory in vivo efficacy results, it seems that the prepared formulation is a promising candidate for further in vivo studies.Graphical abstractGraphical abstract for this article
  • Multi-stimuli responsive mesoporous carbon nano-platform gated by human
           serum albumin for cancer thermo-chemotherapy
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Qinfu Zhao, Xiudan Wang, Ming Yang, Xian Li, Yuling Mao, Xinyao Guan, Donghua Di, Siling Wang In this work, a multi-stimuli responsive drug delivery system (MCHP) was designed for combinational chemotherapy and photothermal therapy (PTT). Mesoporous carbon nanoparticles (MCN) with a high loading efficiency were used as near-infrared (NIR)-responsive drug carriers. Human serum albumin (HSA) was attached to the pore openings of MCN via disulfide bonds to serve as a gatekeeper due to its biocompatibility and appropriate molecular size. To improve the dispersity and biocompatibility, the surface of the MCN was modified with polyethylene glycol (PEG). In vitro photothermal effect results showed that MCHP exhibited a power and concentration-dependent photothermal conversion capacity and a good photothermal stability. The doxorubicin (DOX) release from the MCHP/DOX system exhibited NIR/pH/reduction-responsive release properties. A cytotoxicity assay demonstrated that, under NIR irradiation, the MCHP/DOX exhibited chemo-photothermal synergistic effects with a combination index (CI) of 0.643. The biodistribution of DOX in vivo indicated that an NIR laser can prolong the retardation time of DOX in tumor sites. In vivo antitumor experiments showed that MCHP/DOX with NIR irradiation had the highest tumor inhibition rate against 4T1 tumors in mice. This work suggested that MCHP could be explored as a multi-responsive drug release platform for combinational photothermo-chemotherapy.Graphical abstractGraphical abstract for this article
  • Highly efficient and selective antimicrobial isonicotinylhydrazide-coated
           polyoxometalate-functionalized silver nanoparticles
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Akhela Umapathi, Navya P. Nagaraju, Harishkumar Madhyastha, Devendra Jain, Sangly P. Srinivas, Vincent M. Rotello, Hemant Kumar Daima With the rapidly approaching post-antibiotic era, new and effective combinations of antibiotics are imperative to combat multiple drug resistance (MDR). We have synthesized multimodal antimicrobials that integrate the antibiotic isonicotinylhydrazide (INH), silver nanoparticles (AgNPsINH), and two different polyoxometalates (POMs) namely, phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) to prepare AgNPsINH@PTA and AgNPsINH@PMA, respectively. AgNPsINH have peroxidase-like (nanozyme) activity and very high antibacterial potential toward S. aureus, which was further enhanced upon modification with POMs. The selectivity of these functional nanozymes was evaluated with m5S mouse fibroblasts using WST-8, LDH viability, in vitro reactive oxygen species (ROS) generation assays, and crystal violet morphological studies. These investigations showed very low cytotoxicity for the nanoparticles compared to free metal ions (Ag+), pristine POMs and INH, demonstrating the ability of multifunctional materials to provide efficient and selective antimicrobials.Graphical abstractHighlights•A sequential approach to prepare isonicotinylhydrazide-coated polyoxometalates functionalized Ag nanoparticles is presented.•Isonicotinylhydrazide and polyoxometalates developed a surface corona and imparted biological potential on nanoparticles.•Enhanced in-vitro nanozyme activity, high antimicrobial potential, and controlled cytotoxicity on fibroblasts is reported.Graphical abstract for this article
  • Development of curcumin-loaded methoxy poly(ethylene glycol)-block-
           poly(caprolactone)-block-poly(1, 4, 8-Trioxa [4.6] spiro-9-undecanone)
           nanoparticles and studies on their in vitro anti-tumor activities
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Yongli Shi, Weiwei Ma, Min Gao, Yaxing Yang The purpose of this paper was to fabricate a novel methoxy poly(ethylene glycol)-block-poly(caprolactone)-block-poly(1, 4, 8-Trioxa [4.6] spiro-9-undecanone) (mPEG-b-PCL-b-PTOSUO, mPECT) triblock copolymer and study on the in vitro anti-tumor activity of curcumin-loaded mPECT nanoparticles (NPs). The mPEG-b-PCL-b-PTOSUO NPs were fabricated with solvent evaporation. Transmission electron microscope (TEM) and laser particle analyzer were applied to investigate the morphology and size distribution of the obtained mPECT NPs. The cytotoxicity of the copolymer (mPECT) was reflected by cell viability. Curcumin (CUR), as a model drug, was encapsulated into mPECT NPs. The in vitro anti-tumor activity of CUR-loaded mPECT NPs were also studied. 1H nuclear magnetic resonance (1H NMR), Raman, and Fourier transform infrared spectroscopy (FTIR) spectra confirmed the obtaining of mPECT. TEM photograph showed that most of mPECT NPs were in spherical shapes with a uniform size distribution. High cell viability suggested that the cargo-free mPECT NPs had no obvious cytotoxicity. Fluorescent photographs illustrated that CUR-loaded mPECT NPs could be up-taken by SW1990 cells. The medicated NPs could inhibit the proliferation of SW1990 cells. Therefore, the mPECT NPs could be used as a vehicle to improve the bioavailability and anti-tumor effects of CUR.Graphical abstractGraphical abstract for this article
  • Synthesis and evaluation of acrylate resins suspending indole derivative
           structure in the side chain for marine antifouling
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Kang Feng, Chunhua Ni, Liangmin Yu, Wenjun Zhou, Xia Li A novel indole derivative (N-(1H-2-phenyl-indole-3-ylmethyl) acrylamide, NPI) synthesized by a Friedel-Crafts alkylation reaction was identified using IR spectroscopy, 1H NMR, 13C NMR and elemental analysis. The inhibitory effect of this novel indole derivative on bacteria and marine algae was studied. The results showed that the inhibition ratios of the indole derivative against Escherichia coli and Staphylococcus aureus were 95.93% and 94.91%, respectively, and the indole derivative possessed prominent inhibitory activity against Phaeodactylum tricornutum, Nitzschia Closterium and Skeletonema costatum. These findings indicate that the indole derivative has high biological activity. Subsequently, the indole derivative was introduced to acrylate resins by free-radical polymerization. The resulting acrylate resins were subjected to self-polishing, anti-algal and antifouling test, the results of which indicated that acrylate resins containing the synthesized indole derivative could exhibit significant antifouling properties because of the combination of the biofouling resistance of the indole derivative and the self-polishing properties of acrylate. This work provides an academic foundation for studying environmentally friendly and highly efficient antifouling coatings.Graphical abstractGraphical abstract for this article
  • Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for
           sustained drug delivery
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Sana Sharif, Ghulam Abbas, Muhammad Hanif, Andreas Bernkop-Schnürch, Aamir Jalil, Muhammad Yaqoob It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system. Micro-composites were characterized for preparation yield, size, micromeritic properties and swelling behavior. Chemical composition of micro-composites was characterized by FTIR, XRD and TGA. Scanning electron microscopy (SEM) was used to study their surface morphology. Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug. Preparation yield was found to be in the range of 35.14 ± 1.5–53.97 ± 5.23%. Micro-composites exhibited a mean size range of 0.151 ± 0.49 μm. SEM showed a spherical shape with rough curved porous surface. Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites. HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.Graphical abstractGraphical abstract for this article
  • Advances in biosynthesis of noble metal nanoparticles mediated by
           photosynthetic organisms—A review
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Zeqing Bao, Christopher Q. Lan The last decade has witnessed significant developments in the biosynthesis of noble metal nanoparticles (NMNPs) due to their distinct advantages in various practical applications. Many photosynthetic organisms, including plants, microalgae, and photosynthetic bacteria, have been explored for NMNP synthesis in an eco-friendly and cost-effective manner. These biomasses were used for NMNP biosynthesis as growing cells, non-growing cells, whole cells extract, disrupted cell extract, residual biomasses, gum solutions, etc. Different mechanisms might be involved to reduce noble metal ions to NMNP. These mechanisms include reduction of metal ions catalysed by reductases using NADH as electron donors, reduction of metal ions using biochemical molecules such as polysaccharides and proteins as electron donators, and light-dependant biosynthesis of NMNP involving pigments for light capture and water-splitting for electron supplementation. NMNP may be applied as catalyst, antibacterial, anticancer, and drug delivery vehicle.Graphical abstractGraphical abstract for this article
  • Effects of diameters and crystals of titanium dioxide nanotube arrays on
           blood compatibility and endothelial cell behaviors
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Zhihao Gong, Youdong Hu, Fan Gao, Li Quan, Tao Liu, Tao Gong, Changjiang Pan Titanium dioxide nanotube arrays (TNTAs) have attracted extensive attention in the fields of biomaterials and biomedicine due to their unique tubular structure and good biocompatibility. In this paper, TNTAs with different nanotube diameters and lengths were in situ prepared on the titanium surface by the anodic oxidation, and their crystal structures were further changed by annealing treatment. The effects of TNTAs with different diameters and crystals on the blood compatibility and endothelial cell behaviors were investigated. The results showed that TNTAs with the diameter of 30∼90 nm can be obtained by controlling the anodization voltage, and annealing treatment did not obviously change the diameters and lengths of the nanotube arrays. However, annealing treatment can transform the amorphous TNTAs into the anatase structure. The diameter and crystal structure of the nanotube arrays played a key role in the surface wettability and protein adsorption. The nanotube array with larger diameter displayed better surface hydrophilicity as compared to the pristine titanium, and annealing treatment further enhanced the hydrophilicity. As compared to the pristine titanium, the nanotube array structure had the characteristic of selective protein adsorption, and the nanotube array can promote the bovine serum albumin (BSA) adsorption and prevent the fibrinogen (FIB) adsorption, however, the increase of nanotube diameter could reduce BSA adsorption and increase FIB adsorption. Besides, the nanotube array with anatase structure can promote BSA adsorption while reduce FIB adsorption. Therefore, the TNTAs with smaller diameter and anatase crystal had good blood compatibility and cell compatibility, they can not only reduce platelet adhesion and hemolysis rate but also increase endothelial cell adhesion and proliferation. In conclusion, the nanotube arrays of the present study can be used to improve the cell compatibility and blood compatibility of the titanium implants.Graphical abstractGraphical abstract for this article
  • Enzyme-encapsulating polymeric nanoparticles: A potential adjunctive
           therapy in Pseudomonas aeruginosa biofilm-associated infection treatment
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Chendong Han, James Goodwine, Nicholas Romero, Kyle S. Steck, Karin Sauer, Amber Doiron Pseudomonas aeruginosa is a pathogen known to be associated with a variety of diseases and conditions such as cystic fibrosis, chronic wound infections, and burn wound infections. A novel approach was developed to combat the problem of biofilm antibiotic tolerance by reverting biofilm bacteria back to the planktonic mode of growth. This reversion was achieved through the enzymatic depletion of available pyruvate using pyruvate dehydrogenase, which induced biofilm bacteria to disperse from the surface-associated mode of growth into the surrounding environment. However, direct use of the enzyme in clinical settings is not practical as the enzyme is susceptible to denaturation under various storage conditions. We hypothesize that by encapsulating pyruvate dehydrogenase into degradable, biocompatible poly(lactic-co-glycolic) acid nanoparticles, the activity of the enzyme can be extended to deplete available pyruvate and induce dispersion of mature Pseudomonas aeruginosa biofilms. Several particle formulations were attempted in order to permit the use of the smallest dose of nanoparticles while maintaining pyruvate dehydrogenase activity for an extended time length. The nanoparticles synthesized using the optimal formulation showed an average size of 266.7 ± 1.8 nm. The encapsulation efficiency of pyruvate dehydrogenase was measured at 17.9 ± 1.4%. Most importantly, the optimal formulation dispersed biofilms and exhibited enzymatic activity after being stored at 37 °C for 6 days.Graphical abstractGraphical abstract for this article
  • Conformity of dextran-coated fullerene C70 with L929 fibroblast cells
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): J. Ashtami, S. Anju, P.V. Mohanan Fullerene C70 with symmetric nanostructure and unique properties that open up leeway for both material science and healthcare applications. Poor water dispersity and limited knowledge about its associated toxicity hinders the biomedical scope of C70. These restraining factors need to be addressed. Dextran, natural and water-soluble polymer was used to improve the dispersity of C70 in water. Dextran coating on C70 successfully yielded stable dispersion of C70 in water with remarkable cytocompatibility with L929 fibroblast cells. The dextran-coated C70 was characterized using different characterization techniques such as fourier transformed-infrared spectroscopy (FT-IR), transition electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential. The cell viability assays suggested that the L929 cells retained more than 80% cell viability after 24 h treatment with dextran-coated C70. The mitochontrial membrane potential of the treated cells were found to be uncompromised. Fluorescent imaging techniques done with the aim of evaluating the integrity of lysosomes detected no potential toxicity in L929 cells treated with dextran-coated C70. Actin filaments showed intact organelles when viewed using Rhodamine- phalloidin staining after 24 h post-treatment. DAPI staining also revealed the integrity of nucleus after exposure to dextran-coated C70. The calcein AM/PI flow cytometry analysis further confirmed that dextran-coated C70 kept cell viability of treated cells above 80% for all concentrations. The results points out the scope of dextran-coated C70 for various health care applications.Graphical abstractGraphical abstract for this article
  • Evaluating platelet activation related to the degradation of biomaterials
           using molecular markers
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Sheng Ye, Hong Wang, Fenghua Zhao, Tun Yuan, Jie Liang, Yujiang Fan, Xingdong Zhang The effective assessment of platelet activation is an important component of the evaluation of cardiovascular implants. Currently, most evaluation is performed based on the ISO 10993-4 international standard. However, the methods specified in this standard were originally designed for non-degradable materials, and the applicability of these methods to evaluate degradable materials has not been carefully assessed. Here, the platelet activation response was evaluated (using blood from health rabbits) for three typical degradable materials (collagen, polylactic acid, and hydroxyapatite) by measuring the widely used molecular markers CD62 P, CD63, and CD40 L and the three molecular markers PF4, β-TG, and TXB2 that are referenced in the ISO 10993-4 standard. The variations of these six markers were compared in the simulated degradation of the three test materials. The results showed differences in platelet activation with degradation that were strongly related to the surface physicochemical properties. Changes in the surface roughness and contact angle of the materials correlated with changes in the degree of platelet activation. The six tested platelet activation molecular markers show promise for assessment of platelet function in degradable medical devices, providing guidance for quality control strategies and the design and improvement of safe medical devices.Graphical abstractGraphical abstract for this article
  • The Wnt/β-catenin signaling pathway is regulated by titanium with
           nanotopography to induce osteoblast differentiation
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Rodrigo P.F. Abuna, Fabiola S. Oliveira, Helena B. Lopes, Gileade P. Freitas, Roger R. Fernandes, Adalberto L. Rosa, Marcio M. Beloti Wnt/β-catenin signal transduction is involved in the homeostatic control of bone mass. It is well established that a titanium surface with nanotopography (Ti-Nano) favors osteoblast differentiation by modulating different signaling pathways. However, few studies have investigated the participation of the Wnt/β-catenin pathway in the osteogenic effect of nanoscale topographies. In this study, we aimed to determine whether the Wnt/β-catenin signaling pathway is involved in the elevated osteogenic potential of Ti-Nano. MC3T3-E1 cells were cultured on Ti-Nano and machined Ti (Ti-Control) for evaluation of the expression of Wnt/β-catenin signaling pathway-related genes. Based on the results to real-time PCR, the Wnt receptor Fzd4 was selected and silenced by CRISPRi. The resulting cells were cultured on both Ti surfaces, and several events involved in osteoblast differentiation were evaluated. The results revealed that Fzd4 gene silencing, corresponding to negative modulation of Wnt/β-catenin, inhibits expression of the osteoblast phenotype. It is worthy of note that this inhibitory effect on osteoblast differentiation was more pronounced in cells grown on Ti-Nano compared with those grown on Ti-Control. By disrupting Fzd4 gene expression, we have shown that the elevated osteogenic potential of Ti-Nano is due to activation of the Wnt/β-catenin signaling pathway, which reveals a new mechanism to explain osteoblast differentiation induced by nanotopography. Such an understanding of the intracellular machinery involved in surface guiding of osteoblast fate may contribute to the development of smart biomaterials to modulate the process of implant osseointegration.Graphical abstractGraphical abstract for this article
  • Multifunctional REDV-G-TAT-G-NLS-Cys peptide sequence conjugated gene
           carriers to enhance gene transfection efficiency in endothelial cells
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Qian Li, Xuefang Hao, Huaning Wang, Jintang Guo, Xiang-kui Ren, Shihai Xia, Wencheng Zhang, Yakai Feng Rapid endothelialization on small diameter artificial blood vessels is an effective strategy to facilitate long-term patency and inhibit thrombosis. The gene delivery can enhance the proliferation and migration of endothelial cells (ECs), which is beneficial for rapid endothelialization. REDV-G-TAT-G-NLS-Cys (abbreviated as TP-G) peptide could weakly condense pEGFP-ZNF580 (pZNF580) and transfect ECs, but its transfection efficiency was still very low because of its low positive charge, low stability and weak endosome escape ability. In order to develop more stable and efficient gene carriers with low cytotoxicity, in the present study, we conjugated different amounts of TP-G peptide onto poly(lactide-co-glycolide)-g-polyethylenimine (PLGA-g-PEI) amphiphilic copolymers via a hetero-poly(ethylene glycol) spacer (OPSS-PEG-NHS). The TP-G peptide and PEI could cooperatively and strongly condense pZNF580. The carrier’s cytotoxicity was reduced by the introduction of poly(ethylene glycol) spacer. They condensed pZNF580 to form gene complexes (PPP-TP-G/pZNF580) with suitable size and positive zeta potential for gene delivery. The transfected ECs promoted their migration ability as demonstrated by cell migration assay. The results of cellular uptake and confocal laser scanning microscopy showed significantly high internalization efficiency, endosomal/lysosomal escape and nucleus location of pZNF580 by this multifunctional TP-G peptide sequence conjugated gene delivery system. Furthermore, several inhibitors were used to study the cellular uptake pathways of PPP-TP-G/pZNF580 complexes. The results showed that PPP-TP-G2/Cy5-oligonucleotide complexes exhibited the optimized endocytosis pathways which facilitated for cellular uptake. In conclusion, the multifunctional TP-G peptide conjugated gene carriers could promote the transfection efficiency due to the multifunction of REDV, cell-penetrating peptide and nuclear localization signal in the peptide sequence, which could be a suitable gene carrier for endothelialization.Graphical abstractGraphical abstract for this article
  • Effect of tannic acid on blood components and functions
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Lu Deng, Yanfang Qi, Zonghua Liu, Yun Xi, Wei Xue Tannic acid (TA) has become increasingly important as a biomaterial because of its ability to capture free radicals and form complexes with metal ions. Nevertheless, a systematic evaluation of the blood safety of TA has not been done. Therefore, we studied the effect of TA on erythrocyte morphology and hemolysis at different time points, and the fluorescence and circular dichroism (CD) spectroscopy of TA and fibrinogen. The blood coagulation time of TA was measured by the activated partial thromboplastin time (APTT), plasma prothrombin time (PT), and thrombelastogram (TEG) experiments. We found that higher concentrations of TA could lead to crinkling and hemolysis of erythrocytes, which may have been mediated by the hydrogen bond in TA. Spectroscopic results showed that TA influenced the structure and conformation of fibrinogen. Higher concentrations of TA could shorten the activated partial thromboplastin and plasma prothrombin times, and various concentrations of TA could affect the reaction time, clotting time, clot aggregation speed, and maximum clot strength of whole blood coagulation. These findings provide a basis for the design of TA molecules and the possible application of TA in biomedicine.Graphical abstractGraphical abstract for this article
  • Effect of Ficus carica L. leaves extract loaded gold nanoparticles against
           cisplatin-induced acute kidney injury
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Samah M. El-Sayed, Mehrez E. El-Naggar, Jihan Hussein, Dalia Medhat, Mona El-Banna BackgroundCisplatin (CisPt) is one of the most widely used and highly effective drugs for the treatment of various solid tumors, unfortunately acute kidney injury (AKI) is considered one of its side effects through several mechanisms including production of reactive oxygen species (ROS), pro-inflammatory and pro-fibrotic cytokines. Due to the poor effect of AKI therapy, the use of nanoparticles loaded with natural extracts for delivering to the kidney molecules are desirable.AimThis study aims to investigate the effectiveness of different concentrations of gold nanoparticles (Au-NPs) as a carrier for Ficus carica L. (Fig) leaves extract against CisPt induced AKI.MethodsSeventy male albino rats were used and divided into seven groups. After the experimental period, blood was withdrawn, serum was separated for determination of urea, creatinine, homocystein (Hcy) and folic acid while reduced glutathione (GSH), nitric oxide (NO), malondialdehyde (MDA), total antioxidant capacity (TAC) and hydroxyproline content (Hyp) were evaluated in kidney tissue homogenate.ResultsCisPt induced AKI in rats and results in a significant increase in the levels of serum urea, creatinine, Hcy and kidney Hyp, lipid peroxidation along with a significant reduction of kidney GSH, NO and TAC compared to the control rats. Treatment with Au-NPs and Fig extract particularly in a ratio of (3:2) respectively was shown to improve renal functions with efficient capacity in scavenging ROS and reduced AKI severity.ConclusionAu-NPs enhanced the anti-oxidative properties of the Fig extract in targeting kidney damaged tissue and reduced oxidative toxicity induced by CisPt.Graphical abstractGraphical abstract for this article
  • Mechanism of fibrinogen /microparticle complex deposition on solid
           substrates: Role of pH
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Paulina Żeliszewska, Marta Sadowska, Maria Morga, Zbigniew Adamczyk Deposition kinetics of fibrinogen/polystyrene particle complexes on mica and the silicon/silica substrates was studied using the direct optical and atomic force microscopy. Initially, basic physicochemical characteristics of fibrinogen and the microparticles were acquired using the dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using the streaming potential measurements. Subsequently an efficient method for the preparation of fibrinogen/polymer microparticle complexes characterized by controlled coverage and molecule orientation was developed. It was demonstrated that for a lower suspension concentration the complexes are stable for pH range 3–9 and for a large concentration for pH below 4.5 and above 5.5. This enabled to carry out thorough pH cycling experiments where their isoelectric point was determined to appear at pH 5. Kinetic measurements showed that the deposition rate of the complexes vanished at pH above 5, whereas the kinetics of the positively charged amidine particles, used as control, remained at maximum for pH up to 9. These results were theoretically interpreted using the hybrid random sequential adsorption model. It was confirmed that the deposition kinetics of the complexes can be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This is in contrast to the fibrinogen molecule behavior, which efficiently adsorb on negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting efficient immunoassays governed by the specific antigen/antibody interactions.Graphical abstractGraphical abstract for this article
  • Comparative safety analysis of bactericidal nano-colloids: Assessment of
           potential functional toxicity and radical scavenging action
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Muhammad Uzair Hashmi, Nauman Khalid, Tehseen Alam, Rumeza Hanif, Hussnain A. Janjua Extensive utilization of silver nanoparticles (AgNP) has raised concerns of their safety profile upon interaction with biological system. In past decade, various nanoparticles (NPs) with excellent antimicrobial potential have been synthesized, a majority of which have struggled with the established toxicity in biological systems. The NPs safety is still a hot debate and various strategies are being adopted to overcome this giant limitation. This paper successfully reports comparative toxicity profiles of previously synthesized antimicrobial NPs in our lab and concludes the effectiveness of biologically synthesized NPs for its safe usage in biological systems. In this study, five of our previously synthesized NPs that showed excellent antimicrobial potential were compared for their in vivo toxicity and corresponding radical scavenging activities. Based on lowest morbidity, mortality, weight loss, toxicity and agglomeration profile, best NPs with highest antimicrobial potentials were screened out and used for further biomedical applications. The previously reported NPs used in this study included Aerva javanica synthesized nanoparticles (AjNPs), Heliotropium crispium synthesized nanoparticles (HcNPs), and violacein capped nanoparticles (VNPs), these showed least toxicity upon in vivo histological analysis. AjNPs among them showed maximum safety and efficacy profile and consistently showed least production of reactive oxygen species, least mortality and morbidity rate as compared to other groups. Present study establishes that all these biologically synthesized NPs and specifically AjNPs can be efficiently employed as antimicrobial agents as they have not exhibited toxic profile and have shown least accumulation into the organs such as liver spleen and kidney.Graphical abstractGraphical abstract for this article
  • Dendrimer grafted albumin nanoparticles for the treatment of post cerebral
           stroke damages: A proof of concept study
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Deepak Pradhan, Vishakha Tambe, Nidhi Raval, Piyush Gondalia, Pallab Bhattacharya, Kiran Kalia, Rakesh K. Tekade Stroke is the second largest disease of mortality. The biggest hurdle in designing effective brain drug delivery systems is offered by the blood-brain barrier (BBB), which is highly impermeable to many drugs. Albumin nanoparticles (NP) have gained attention due to their multiple ligand binding sites and long circulatory half-life. Citicoline (CIT) is reported to enhance the acetylcholine secretion in the brain and also helps in membrane repair and regeneration. However, the poor BBB permeation of CIT results in lower levels of CIT in the brain. This demands the development of a suitable delivery platform to completely realize the therapeutic benefit of CIT in stroke therapy. This investigation reports the synthesis and characterization of second generation (2.0 G) dendrimer Amplified Albumin (dAA) biopolymer by FTIR, MALDI-TOF, and surface charge (mV). Further, the synthesized biopolymer has been utilized to develop a CIT nanoformulation using a commercially translatable one-pot process. Release of CIT from biopolymer was performed within an acetate buffer at pH 5 and Phosphate buffer at pH 7.4. Further, we investigated the ability of biopolymer to permeate BBB by in vitro permeability assay in bEnd.3 cells. MTT assay of CIT-dAA-NP, CIT-ANP, and 2.0 G PAMAM dendrimers was performed in bEnd.3 cells. Therapeutic efficacy of the synthesized biopolymer was determined by VEGF gene expression within an in vitro hypoxia model in PC12 cells. Thus, this investigation resulted in biopolymers that can be used to deliver any therapeutic agent by altering the permeability of the BBB. Also, cationization by dendrimer grafting is one such strategy that may be used to cationize any other negatively charged polymer, such as albumin. The synthesized biopolymer is not limited to deliver molecules to the brain, but can also be used to increase the loading of negatively-charged drug molecules, siRNA, or any other oligonucleotide.Graphical abstractGraphical abstract for this article
  • Inhibited enzymatic reaction of crosslinked lactate oxidase through a
           pH-dependent mechanism
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Hugo Cunha-Silva, F. Pires, A.C. Dias-Cabral, M. Julia Arcos-Martinez Lactate oxidase (LOx), recognized to selectively catalyze the lactate oxidation in complex matrices, has been highlighted as preferable biorecognition element for the development of lactate biosensors. In a previous work, we have demonstrated that LOx crosslinking on a modified screen-printed electrode results in a dual range lactate biosensor, with one of the analysis linear range (4 to 50 mM) compatible with lactate sweat levels. It was advanced that such behavior results from an atypical substrate inhibition process. To understand such inhibition phenomena, this work relies in the study of LOx structure when submitted to increased substrate concentrations. The results found by fluorescence spectroscopy and dynamic light scattering of LOx solutions, evidenced conformational changes of the enzyme, occurring in presence of inhibitory substrate concentrations. Therefore, the inhibition behavior found at the biosensor, is an outcome of LOx structural alterations as result of a pH-dependent mechanism promoted at high substrate concentrations.Graphical abstractGraphical abstract for this article
  • CD19-targeted, Raman tagged gold nanourchins as theranostic agents against
           acute lymphoblastic leukemia
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Andra-Sorina Tatar, Ancuta Jurj, Ciprian Tomuleasa, Adrian Florea, Ioana Berindan-Neagoe, Dana Cialla-May, Juergen Popp, Simion Astilean, Sanda Boca The incidence of Acute Lymphoblastic Leukemia (ALL) is increasing globally, and it is being clinically addressed by chemotherapy, followed by immunotherapy and stem cell transplantation, all with potential life-threatening toxicities. In the need for more effective therapeutics, newly developed disease-targeted nanocompounds can thus hold real potential.In this paper, we propose a novel nanoparticle-based immunotherapeutic agent against ALL, consisting of antiCD19 antibody-conjugated, polyethylene glycol (PEG)-biocompatibilized, and Nile Blue (NB) Raman reporter-tagged gold nanoparticles of urchin-like shape (GNUs), that have a plasmonic response in the Near Infrared (NIR) spectral range. Transmission electron microscopy (TEM) images of particle-incubated CD19-positive (CD19(+)) CCRF-SB cells show that the antiCD19-PEG-NB-GNU nanocomplex is able to recognize the CD19 B-cell-specific antigen, which is a prerequisite for targeted therapy. The therapeutic effect of the particles is confirmed by cell counting, combined with cell cycle analysis by flow cytometry and MTS assay, which additionally offer insights into their mechanisms of action. Specifically, antiCD19-PEG-NB-GNUs proved superior cytotoxic effect against CCRF-SB cells when compared with the free antibody, by reducing the overall viability below 18% after 7 days treatment at a particle-bound antibody concentration of 0.17 ng/μl. Moreover, by combining their remarkable plasmonic properties with the possibility of Raman tagging, the proposed nanoparticles can also serve as spectroscopic imaging agents inside living cells, which validates their theranostic potential in the field of hematological oncology.Graphical abstractGraphical abstract for this article
  • Development and in vitro assessment of an anti-tumor
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Claire K. Holley, You Jung Kang, Chung-Fan Kuo, Mohammad Reza Abidian, Sheereen Majd This study aims to develop a new anti-cancer formulation based on the chelator Dp44mT (Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone). Dp44mT has outstanding anti-tumor activity and the unique ability to overcome multidrug-resistance in cancer cells. This highly toxic compound has thus far only been applied in free form, limiting its therapeutic effectiveness. To reach its full therapeutic potential, however, Dp44mT should be encapsulated in a nano-carrier that would enable its selective and controlled delivery to malignant cells. As the first step towards this goal, here we encapsulate Dp44mT in nanoparticles (NPs) of poly(lactic-co-glycolic acid) (PLGA), characterize this nano-formulation, and evaluate its therapeutic potential against cancer cells in vitro. Our results showed that the Dp44mT-loaded NPs were homogenous in shape and size, and had good colloidal stability. These PLGA NPs also showed high encapsulation efficiency and loading capacity for Dp44mT and enabled the sustained and tunable release of this chelator. Dp44mT-NPs were uptaken by cancer cells, showed a strong and dose-dependent cytotoxicity towards these cells, and significantly increased apoptotic cell death, in both monolayer and spheroid tumor models. This formulation had a low-level of toxicity towards healthy control cells, indicating an inherent selectivity towards malignant cells. These results demonstrate the great potential of this novel Dp44mT-based nano-formulation for the use in cancer therapy.Graphical abstractGraphical abstract for this article
  • Dual-functional Supramolecular Nanohybrids of Quantum
           Dot/Biopolymer/Chemotherapeutic Drug for Bioimaging and Killing Brain
           Cancer Cells in vitro
    • Abstract: Publication date: Available online 14 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Alexandra A.P. Mansur, Anderson J. Caires, Sandhra M. Carvalho, Nadia S.V. Capanema, Isadora C. Carvalho, Herman S. Mansur Glioblastoma (GBM) is the utmost aggressive and lethal primary brain cancer, which has a poor prognosis and remains virtually incurable. Nanomedicine with emerging disruptive nanotechnology alternatives, including designed supramolecular nanohybrids has excellent potential as multimodal tools against cancer by combining nanomaterials, biomacromolecules, and drugs. Thus, we developed and constructed for the first time quantum dot-biopolymer-drug nanohybrids based on host-guest chemistry for simultaneous bioimaging, targeting, and anti-cancer drug delivery against GBM cells in vitro. ZnS fluorescent quantum dots (ZnS-QDs) were produced using chemically modified polysaccharide, carboxymethylcellulose (CMC), as water-soluble capping ligand and biofunctional layer via a facile one-step eco-friendly aqueous colloidal process at room temperature and physiological pH. These hybrid inorganic-organic nanocolloids (ZnS@CMC) were electrostatically conjugated with doxorubicin (DOX) anti-cancer drug forming innovative supramolecular complexes (ZnS@CMC-DOX) for amalgamating bioimaging and killing cancer cells. These nanoconjugates were characterized regarding their optical and physicochemical properties combined with morphological and structural features. The cytocompatibility was evaluated by MTT assay using healthy and GBM cells. The results showed that ultra-small ZnS-QDs were expertly produced uniform nanocolloids (average size = 3.6 nm). They demonstrated photoluminescence emission within the visible range of spectra. The cell viability results in vitro showed no cytotoxicity of ZnS@CMC nanohybrids towards both cell types. In summary, the novelty of this research relies on using a nanotheranostic strategy for developing ZnS@CMC-DOX nanohybrids with supramolecular vesicle-like structures. They behaved simultaneously as active fluorescent nanoprobes and nanocarriers with modulated drug release for bioimaging and killing malignant glioma cells proving the high potential for applications in cancer nanomedicine.Graphical Graphical abstract for this article
  • In situ assembly of well-dispersed Ag nanoparticles on the surface of
    • Abstract: Publication date: Available online 13 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Qingmiao Zhang, Yilei Wang, Wenkun Zhang, Michael E. Hickey, Zhuangsheng Lin, Qin Tu, Jinyi Wang Nanofibrous membranes which exhibit bacteriostatic functions are a good strategy to prevent microorganisms from adhering to the surface of biomaterials. Here, we report the synthesis of such a nanofibrous membrane which can be applied to biological coatings to reduce bacteriostatic functionality. Ascorbic acid was utilized to reduced chloroauric acid to gold nanoparticles (AuNPs). Dopamine was then polymerized upon AuNP surfaces by ultrasound-assistance, to synthesize core-shell structured polydopamine-coated AuNPs (Au@PDA NPs). The Au@PDA NPs were then mixed with polylactic acid (PLA) for electrospinning into cylindrical nanofibers (136.6 nm diameter). PLA-Au@PDA nanofibrous membranes were finally immersed in silver nitrate for in situ reduction into a silver nanoparticle (AgNP) coating to yield PLA-Au@PDA@Ag nanofibers. The PLA-Au@PDA@Ag nanofibers were characterized based on field emission scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and contact angle analyses. The antibacterial properties of the PLA-Au@PDA@Ag nanofibers were examined based on the optical density absorbance of bacterial cell suspensions, traditional colony plate counts, zone inhibition analyses, and field-emission scanning electron microscopy. Escherichia coli and Staphylococcus aureaus respectively served as Gram negative and positive bacterial models of industrial relevance. The data conclusively illustrates the antimicrobial and biomedical applications of PLA-Au@PDA@Ag nanofibers.Graphical Graphical abstract for this article
  • Eco-scalable baicalin loaded vesicles developed by combining phospholipid
           with ethanol, glycerol, and propylene glycol to enhance skin permeation
           and protection
    • Abstract: Publication date: Available online 12 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Maria Manconi, Carla Caddeo, Amparo Nacher, Octavio Diez-Sales, Josè Esteban Peris, Elvira Escribano Ferrer, Anna Maria Fadda, Maria Letizia Manca A new class of biocompatible and scalable phospholipid vesicles was developed, aiming at improving the efficacy of baicalin on the skin. Phosphatidylcholine and baicalin (a natural polyphenol) were hydrated in two steps with a mixture of ethanol, glycerol, and propylene glycol at different ratios, and a low amount of water (4%). Hence, water was almost completely replaced by the co-solvents, which were never used before as predominant dispersing medium of phospholipid vesicles. The vesicles appeared three-dimensionally structured, forming a network that conferred a high viscosity to the dispersions. The vesicles were unilamellar, small in size (∼100 nm), and stable during 12 months of storage. They disclosed optimal performances in the transdermal delivery of baicalin, and high biocompatibility with skin cells (i.e., keratinocytes and fibroblasts). Furthermore, the vesicles promoted the efficacy of baicalin in protecting skin cells against oxidative stress in vitro and injured skin in vivo.Graphical abstractGraphical abstract for this article
  • Role of trapped air and lubricant in the interactions between fouling and
           SiO2 nanoparticle surfaces
    • Abstract: Publication date: Available online 11 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Xiaoyan He, Feng Tian, Xiuqin Bai, Chengqing Yuan Both biomimetic superhydrophobic surfaces and biomimetic slippery liquid-infused porous surfaces (SLIPSs) have been developed as potential alternatives for solving the problem of biofouling. Herein, a facile method was used to construct superhydrophobic surfaces and liquid infused porous surfaces on stainless steels for antifouling applications. The nano-structures were formed by electrostatic attraction between polycations and negatively charged SiO2 nanoparticles, providing a structural basis for superhydrophobic surfaces and liquid infused surfaces. Biofouling testing suggested excellent antifouling performances of the liquid infused porous surfaces by decreasing the adhesion of Chlorella pyrenoidosa by 93% and of Phaeodactylum tricornutum by 71%. The thermodynamic interpretation further indicated that the air layer captured by the superhydrophobic surfaces and the lubricant layer entrapped by the liquid infused porous surfaces played the dominant role in their antifouling performances. The inspiring results might show great potential for liquid infused porous surfaces in antifouling applications.Graphical abstractGraphical abstract for this article
  • Nanorods to hexagonal nanosheets of CuO-doped manganese oxide
           nanostructures for higher electrochemical supercapacitor performance
    • Abstract: Publication date: Available online 11 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): H.M. Yadav, G.S. Ghodake, D.-Y. Kim, Sivalingam Ramesh, N.C. Maile, D.S. Lee, S.K. Shinde In this work, the extraordinary properties of CuO addition on the morphology and supercapacitive performance of Mn2O3 electrodes were demonstrated. Concisely, CuO/Mn2O3 thin films were prepared by an easy and inexpensive successive ionic layer adsorption and reaction (SILAR) method. The prepared thin films were characterized by various sophisticated physiochemical systems. The results demonstrated formation of Mn2O3 thin films with noteworthy morphological alteration upon introduction of CuO. Furthermore, a significant effect of CuO introduction was observed on the electrocatalytic properties of the nanostructured Mn2O3 electrodes. At 3% CuO doping, the Mn2O3 electrodes displayed the maximum specific capacitance owing to formation of nanoplate-like structures. The enhanced specific capacitance attained for 3% CuO doping in the Mn2O3 electrode was 500 F/g at 5 mV/s in a 3 M KOH electrolyte. All results confirmed the plausible potential of the CuO/Mn2O3 electrode for supercapacitor applications.Graphical abstractGraphical abstract for this articleCV and SEM images of 3% CuO doped Mn2O3 thin films synthesized by SILAR method.
  • Formation lipase cross-linked enzyme aggregates on octyl-modified
           mesocellular foams with oxidized sodium alginate
    • Abstract: Publication date: Available online 11 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Wenbin Jin, Yan Xu, Xiao-Wei Yu Supported cross-linked enzyme aggregates were prepared by immobilization of Candida antarctica lipase B onto hydrophobic surface of octyl-modified mesocellular foams (MCFs-C8). Oxidized sodium alginate was used as a substitute for traditional glutaraldehyde. Supported cross-linked enzyme aggregates using oxidized sodium alginate (SA-CLEAs@MCFs-C8) exhibited significantly improved thermal stability and organic solvents tolerance compared to the free lipase, lipase adsorbed onto MCFs-C8 and supported cross-linked enzyme aggregates using glutaraldehyde (G-CLEAs@MCFs-C8). Then immobilized lipases were employed for biodiesel production by transesterification of soybean oil with methanol. In the optimization condition, SA-CLEAs@MCFs-C8 were quite stable and still showed high fatty acid methyl esters (FAME) yield after 5 repeated cycles (from 89% to 78%), whereas MCFs-C8-CALB retained 67% FAME yield (about 72% for G-CLEAs@MCFs-C8).Graphical abstractGraphical abstract for this article
  • The correlation between the methylation of PTEN gene and the apoptosis of
           osteosarcoma cells mediated by SeHA nanoparticles
    • Abstract: Publication date: Available online 10 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yanhua Wang, Na Qin, Caifa Zhao, Jiehua Yuan, Shiqi Lu, Wenjing Li, Huiyao Xiang, Hang Hao The invasive spreading of residual osteosarcoma cells becomes a serious threat to human health, urgently needing new bone regenerative biomaterials for orthopedic therapy. Thus, in this work, selenite-substituted hydroxyapatite (SeHA) nanoparticles were prepared for both inhibiting the recurrence of the tumor and accelerating the regenerative repair of bone defect. Physicochemical characterization showed these synthetic nanoparticles were spherical poly-crystals with the shape of snowflakes. Such structure benefited them to inhibit the cellular viability of osteosarcoma cells by about (58.90 ± 14.37)% during 48 h co-culturing. The expression level of cell growth-related genes such as PTEN, MMP-9, Cyclin D1, Cyclin A2, Annexin A2 and CDC2 decreased. Bisulfite Sequence PCR of PTEN gene exhibited about (22.40 ± 5.39)%, (45.91 ± 6.36)% and (25.90 ± 5.36)% promoter methylation in control, HA and SeHA group. Animal experiment also proved the similar effects. Almost no recurrence were observed in SeHA group. Oppositely, the slowly recurrent growth of the remnant tumor appeared in purely surgical group. The overall survival and toxicity analysis showed that, in the usage dose of 0–0.1 g, the SeHA-0.01 exhibited higher inhibitory recurrence and metastasis potentials, lower renal toxicity and better anti-inflammation function. Immunohistochemistry stain showed the reduced expression of PTEN, MMP-9, Ki-67 and Annexin A2, but slightly increased expression of DNMT1 and BMP-2. Compared the methylation status of PTEN gene in each group, it was confirming that SeHA nanoparticles hardly possessed the de-methylation effect, but the pure HA strikingly increased the methylation level of such gene. It seemed the dopant selenite ions possessed de-methylation effect onto PTEN gene. Therefore, from the viewpoint of inhibiting metastatic potentials, the SeHA-0.01 might be a feasible biomaterial to inhibit the relapse of the tumor post-surgery.Graphical abstractGraphical abstract for this article
  • Enzymatic degradation of the polymer capsules with a hydrophobic core in
           the presence of Langmuir lipid monolayer as a model of the cellular
    • Abstract: Publication date: 1 December 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 184Author(s): Evgenii P. Mironov, Tatiana N. Borodina, Daria G. Yurina, Daria B. Trushina, Tatiana V. Bukreeva Submicrocapsules were prepared from diethylaminoethyl dextran (DEAE-D), xanthan gum (XG) and bovine serum albumin (BSA) on oil cores by ultrasonic treatment. These capsules were modified with poly-L-lysine (PLL) via electrostatic adsorption. The behavior of the capsules was investigated at an air–water interface after their introduction into an aqueous subphase. The interaction of the capsules with 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) monolayer formed on the water surface (model cellular membrane) was studied both upon their introduction under the condensed monolayer and with the use of a dilute colloidal solution of the capsules as a subphase. Biodegradation of the proteinaceous capsules with subsequent oil-core release was demonstrated by influence of pronase. The Langmuir lipid monolayer was found to be a good model for investigation of drug release from the capsules in the presence of the cellular membrane.Graphical abstractGraphical abstract for this article
  • Sorafenib encapsulated in nanocarrier functionalized with glypican-3
           specific peptide for targeted therapy of hepatocellular carcinoma
    • Abstract: Publication date: Available online 9 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Shuo Feng, Juan Zhou, Zhao Li, Henry D. Appelman, Lili Zhao, Jiye Zhu, Thomas D. Wang Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world with increasing incidence. Chemotherapy is required for HCC patients after receiving surgical resection. Serious off-target induced side effects and systemic toxicity limit the clinical utility of drugs. Targeting therapeutic nanomedicine is an innovative strategy for enhancing drug delivery efficiency and reducing side effects. Here, we successfully formulated nanocarriers to encapsulate sorafenib, an FDA approved drug for treatment of HCC. Sorafenib is encapsulated with an entrapment efficiency>80% over 20 days. The effective aqueous solubility is improved over 1900-fold. The release ratio in vitro is characterized by a half-life of T1/2 = 22.7 hours. The peak target-to-background ratio for nanocarrier uptake by tumor occurs at 24 hours post-injection, and is significantly greater for the target peptide versus controls. Ex vivo biodistribution confirms the in vivo results. Tumor regression is significantly greater for the target peptide versus controls after 21 days of therapy. No acute toxicity is found by blood chemistry or necropsy. In summary, a peptide specific for GPC3 has been identified, and used to modify the surface of a nanocarrier that encapsulates sorafenib with high entrapment efficiency. Regression of HCC xenograft tumors showed promise for targeted drug delivery.Graphical abstractGraphical abstract for this article
  • Controlling Complexation/Decomplexation and Sizes of Polymer-based
           Electrostatic pDNA Polyplexes is One of the Key Factors in Effective
    • Abstract: Publication date: Available online 9 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Kyoungnam Kim, Hee Sook Hwang, Min Suk Shim, Yong-Yeon Cho, Joo Young Lee, Hye Suk Lee, Han Chang KangABSTRACTThe delivery of plasmid DNA (pDNA) using polycations has been investigated for several decades; however, obstacles that limit efficient gene delivery still hinder the clinical application of gene therapy. One of the major limiting factors is controlling DNA binding affinity with polymers to control the complexation and decomplexation of polyplexes. To address this challenge, polycations of α-poly(L-lysine) (APL) and ε-poly(L-lysine) (EPL) were used to prepare variable complexation/decomplexation polyplexes with binding affinities ranging from too tight to too loose and sizes ranging from small to large. APL-EPL/ATP-pDNA polyplexes were also prepared to compare the effects of endosomolytic ATP on complexation/decomplexation and the sizes of polyplexes. The results showed that smaller and tighter polyplexes delivered more pDNA into the cells and into the nucleus than the larger and looser polyplexes. Larger polyplexes exhibited slower cytosolic transport and consequently less nuclear delivery of pDNA than smaller polyplexes. Tighter polyplexes exhibited poor pDNA release in the nucleus, leading to no improvement in transfection efficiency. Thus, polyplexes should maintain a balance between complexation and decomplexation and should have optimal sizes for effective cellular uptake, cytosolic transport, nuclear import, and gene expression. Thus, understanding the effects of complexation/decomplexation and size is important when designing effective polymer-based electrostatic gene carriers.Graphical Graphical abstract for this article
  • Proof of concept of plasmonic thermal destruction of surface cancers by
           gold nanoparticles obtained by green chemistry
    • Abstract: Publication date: Available online 8 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Maroua Ben Haddada, Dimitri Koshel, Zhang Yang, Weiling Fu, Jolanda Spadavecchia, Sabrina Pesnel, Anne-Laure Morel A greener approach for the design of surface plasmon resonant gold nanoparticles has been obtained with a hydrosoluble fraction of an endemic asteraceae medicinal plant. This medicinal plant is originated from Indian Ocean and demonstrates its bioreducing activity in the design of stable green nanomedicine in aqueous media. This article describes the preclinical assessment of the efficacy of these novel nanocandidates on murine model by intratumoral and intravenous injections. It definitely demonstrates two key points in the treatment of cancer: 1) optimization of the tumor microenvironment targeting by specific ligands for a limited damage on healthy tissue, 2) the need to screen the specific irradiation dose (time, power) taking into account the type of tumor.Graphical abstractGraphical abstract for this article
  • Light-Activated Doxorubicin-Encapsulated Perfluorocarbon Nanodroplets For
           On-Demand Drug Delivery in an in vitro Angiogenesis Model: Comparison
           Between Perfluoropentane and Perfluorohexane
    • Abstract: Publication date: Available online 7 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zheng Yuan, Alec Demith, Ryan Stoffel, Zhe Zhang, Yoonjee C. Park Phase-transition perfluorocarbon (PFC) nanodroplets have been developed for on-demand drug delivery carriers with external triggers such as ultrasound or laser irradiation techniques. Although various perfluorocarbons, including perfluoropentane (C5F12) and perfluorohexane (C6F14), have been investigated for their theranostic use, comparison of the phase-transition efficiency, the drug delivery efficacy by light activation, and physical properties of the PFC nanodroplets have not been reported. We have synthesized gold nanorod-coated doxorubicin-encapsulated perfluorocarbon nanodroplets using perfluoropentane and perfluorohexane as light-activated on-demand drug delivery carriers, called PF5 and PF6, respectively. When gold nanorods on the perfluorocarbon nanodroplets resonate with a laser wavelength, plasmonic heat generated on the gold nanorods vaporizes the nanodroplets to gas bubbles (phase-transition), and releases the encapsulated drug from the nanodroplet core.Overall, the nanodroplet size, drug encapsulation efficiency, number density, and cytotoxicity were similar between PF5 and PF6. However, the long-term stability against passive phase-transition or coalescence in physiological conditions and the phase-transition efficiency were different from each other. PF6 was better in long-term stability but showed lower phase-transition than PF5. The lower phase-transition of PF6 might have led to lower drug delivery efficiency compared to PF5. This is probably because PF6 has higher temperature thresholds required for phase-transition due to its higher boiling point. The study demonstrated feasibility of the light-activated nanodroplets for on-demand targeted nanotherapy, which suppresses the development of angiogenesis.Graphical Graphical abstract for this article
  • Hydroxytyrosol encapsulated in biocompatible water-in-oil microemulsions:
           How the structure affects in vitro absorption
    • Abstract: Publication date: Available online 7 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Evgenia Mitsou, Adeline Dupin, Abdessattar Hadj Sassi, Julien Monteil, George T. Sotiroudis, Fernando Leal-Calderon, Aristotelis Xenakis Over the last years, the incorporation of natural antioxidants in food and pharmaceutical formulations has gained attention, delaying or preventing oxidation phenomena in the final products. In order to take full advantage of their properties, protection in special microenvironments is of great importance. The unique features of the natural phenolic compound hydroxytyrosol (HT) - including antioxidant, anti-inflammatory, antiproliferative and cardioprotective properties - have been studied to clarify its mechanism of action. In the present study novel biocompatible water-in-oil (W/O) microemulsions were developed as hosts for HT and subsequently examined for their absorption profile following their oral uptake. The absorption of HT in solution was compared with the encapsulated one in vitro, using a coculture model (Caco-2/TC7 and HT29-MTX cell lines). The systems were structurally characterized by means of Dynamic Light Scattering (DLS) and Electron Paramagnetic Resonance (EPR) techniques. The diameter of the micelles remained unaltered after the incorporation of 678 ppm of HT but the interfacial properties were slightly affected, indicating the involvement of the HT molecules in the surfactant monolayer. EPR was used towards a lipophilic stable free radial, namely galvinoxyl, indicating a high scavenging activity of the systems and encapsulated HT. Finally, after the biocompatibility study of the microemulsions the intestinal absorption of the encapsulated HT was compared with its aqueous solution in vitro. The higher the surfactants’ concentration in the system the lower the HT concentration that penetrated the constructed epithelium, indicating the involvement of the amphiphiles in the antioxidant’s absorption and its entrapment in the mucus layer.Graphical abstractGraphical abstract for this article
  • Multichannel hydrogel based on a chitosan–poly(vinyl alcohol)
           composition for directed growth of animal cells
    • Abstract: Publication date: Available online 7 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anastasia V. Sochilina, Nikita Y. Budylin, Alina M. Gamisonia, Anatoly E. Chalykh, Vitaly P. Zubov, Alexander A. Vikhrov In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan–poly(vinyl alcohol) compositions was developed. Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve. Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions. A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve. In vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.Graphical Graphical abstract for this article
  • Double porous poly (Ɛ- caprolactone)/chitosan membrane scaffolds as
           niches for HUMAN Mesenchymal Stem CellS
    • Abstract: Publication date: Available online 6 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Pritam Das, Simona Salerno, Jean-Christophe Remigy, Jean-François Lahitte, Patrice Bacchin, Loredana De Bartolo In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues. The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells. We developed poly (Ɛ- caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds. We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30). Membranes exhibited macrovoids connected with each other through a microporous network; macrovoids size increased by increasing the CHT wt%. Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.The scaffolds were able to create a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity. We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area. The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.Graphical abstractGraphical abstract for this article
  • Electrochemical behavior of CoCrMo alloy for dental applications in acidic
           artificial saliva containing albumin
    • Abstract: Publication date: Available online 6 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Lixia Yang, Qiliang Zhu, Xiaochun Xie, Xueli Cao, Yuntao Wu, Shuo Chen, Jun-e Qu The electrochemical properties of CoCrMo alloy immersed in different artificial saliva with or without Ca2+ and albumin were studied by open circuit potential (OCP), electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PP), and meanwhile the microstructures features, phase identification and chemical composition of the alloy were analyzed by SEM, EDS, XRD and RA-IR to further understand the electrochemical behavior of the alloy. The results indicated that the self-passivation behavior of the alloy occurred universally and was obviously distinct with each other in different acidic artificial saliva. No phase transformation was observed and the oxide layer and corrosion products exhibited amorphous nature. There was an obvious complexation of the adsorbed albumin with the alloy, and the adsorption capacity of albumin increased with the prolongation of immersion time. The adsorbed albumin presenting black stripped spots had a certain inhibition to the formation of passivation film, and Ca2+ in saliva promoted the further adsorption of albumin as an intermediate bridge, going against the improvement of the corrosion resistance of passivation film/alloy system. In addition, the passivation state of the alloy surface was changed by different methods to investigate the adsorption behavior of albumin and its influence on the further passivation behavior in depth. Hitherto, we tried to propose a model to explain the dynamic adsorption process of albumin and its influencing mechanism on the growth behavior of passivation film.Graphical abstractGraphical abstract for this article
  • Effects of gold nanostructures on differentiation of mesenchymal stem
    • Abstract: Publication date: Available online 6 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Long Yuan, Xiaowei Qi, Gaoping Qin, Qi Liu, Fan Zhang, Yong Song, Jun Deng Nanoparticles are nanocrystals with complex facets and defective structures that do not adopt an idealised shape. Various physicochemical parameters of nanoparticles, such as surface composition, size, and stiffness, can regulate differentiation in mesenchymal stem cells (MSCs), but the influence of shapes with many edges and corner regions has not been investigated. Herein, we investigated the effects of two gold nanostructures modified with 11-mercaptoundecanoic acid, namely gold nanocubes (MUA-AuNCs) and nanooctahedras (MUA-AuNOs), on viability and differentiation in rat bone marrow MSCs (bMSCs). Analysis of cytotoxicity and proliferation demonstrated good biocompatibility, with concentrations
  • Chiral mesoporous silica based LOFL delivery systems using achiral
           alcohols as co-structure directing agents: construction, characterization,
           sustained release and antibacterial activity
    • Abstract: Publication date: Available online 5 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Mingshu Cui, Luyao Xie, Shiwen Zhang, Lu Chen, Yanru Xi, Yumei Wang, Yingyu Guo, Lu Xu The present work reported two synthesized chiral mesoporous silicas (CMSs) with opposite chirality for loading, release, and antibacterial activities of levofloxacin (LOFL). Herein, helical CMS nanorods were prepared by the sol-gel method using CTAB as a template and either n-heptanol or n-nonanol as a co-structure directing agent (CSDA). The synthesized CMSs were characterised by transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS), induced circular dichroism (ICD), and nitrogen adsorption/desorption. X-ray diffraction (XRD) was applied to confirm amorphous state transformation after drug loading, and thermal gravimetric analysis (TGA) was used to quantify the LOFL loading capacity. In vitro drug release studies from the loaded CMSs showed significant differences in the release behaviour for LOFL. Moreover, LOFL-loaded CMSs significantly sustained LOFL release with Fickian diffusion mechanism. Both the drug-loaded CMSs inhibited bacterial growth successfully when tested with strains of E. coli and S. aureus. Therefore, the prepared CMSs could find application as suitable vehicles for drug delivery systems to exert the therapeutic effects of LOFL and achieve different release behaviours.Graphical abstractGraphical abstract for this article
  • Tracing Upconversion Nanoparticle Penetration in Human Skin
    • Abstract: Publication date: Available online 5 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zahra Khabir, Anna E. Guller, Vlada S. Rozova, Liuen Liang, Yi-Jen Lai, Ewa M. Goldys, Honghua Hu, Karen Vickery, Andrei V. Zvyagin Due to their unique optical properties upconversion nanoparticles (UCNPs) provide exceptionally high contrast for imaging of true nanoparticle distribution in excised human skin. It makes possible to show penetration of solid nanoparticles in skin treated with chemical enhancers. We demonstrated tracing upconversion nanoparticles in excised human skin by means of optical microscopy at the discrete particle level sensitivity to obtain their penetration profiles, which was validated by laser-ablation inductively-coupled-plasma mass-spectrometry. To demonstrate utilities of our method, UCNPs were coated with polymers, formulated in water and chemical enhancers, and applied on excised human skin mounted on Franz cells, followed by imaging using a custom-built laser-scanning microscope. To evaluate the toxicity impact on skin by polymer-coated UCNPs, we introduced a tissue engineering model of viable epidermis made of decellularized chick embryo skin seeded with keratinocytes. UCNPs formulated in water stopped in stratum corneum, whereas UCNPs formulated in ethanol-water solution crossed stratum corneum and reached viable epidermis – hence, the enhancement effect for solid nanoparticles was detected by optical microscopy. All polymer-coated UCNPs were found nontoxic within the accepted safety levels. The keratinocyte resilience to polyethyleneimine-coated UCNPs was surprising considering cytotoxicity of polyethyleneimine to two-dimensional cell cultures.Graphical Graphical abstract for this article
  • Bioparticles of flaxseed protein and mucilage enhance the physical and
           oxidative stability of flaxseed oil emulsions as a potential natural
           alternative for synthetic surfactants
    • Abstract: Publication date: Available online 4 September 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Maryam Nikbakht Nasrabadi, Sayed Amir Hossein Goli, Ali Sedaghat Doost, Koen Dewettinck, Paul Van der Meeren Flaxseed protein (FP) and mucilage (FM) complex bioparticles as sustainable ingredients were assembled by electrostatic interaction for plant-based Pickering stabilization of flaxseed oil (FO)-in-water emulsions. The effect of FO content (1 - 5 wt%) on droplet size and accelerated creaming stability of the emulsions was evaluated, from which it was found that 2.5 wt% FO emulsion had the smallest initial droplet size (i.e. D[4,3] = 8 µm) and creaming velocity (2.9 µm/s). The microstructure of the emulsions was observed using Cryo-SEM, confocal and optical microscopy, showing a thick layer of the particles on the oil surface responsible for the stabilization. The physical stability of FO emulsions stabilized by complex bioparticles against various environmental stress conditions (pH, salt and temperature) was higher compared to plain FP- and polysorbate 80-stabilized emulsions. Thus, the droplet size of FP-stabilized emulsions (pH 3) increased from 21 to 38 µm after thermal treatment (80 °C), whereas the size distribution of particle-stabilized emulsions hardly changed. The latter emulsions also remained stable during 28 days of storage and displayed good stability against a wide range of pH conditions (2-9) and salt concentrations (0-500 mM) with no sign of oiling-off. The complex particles as Pickering emulsifiers were successful to depress the FO oxidation at 4 °C and 50 °C. This study could open a promising pathway for producing natural and surfactant-free emulsions through Pickering stabilization using plant-based biopolymer particles for protecting lipophilic bioactive components.Graphical abstractGraphical abstract for this article
  • Easy-Cleaning Surfaces Functionalized with an Interface-Binding
           Recombinant Lipase
    • Abstract: Publication date: Available online 10 August 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Liting Zhang, Yaofei Sun, Yibing Wang, Xiaoli Wang, Haifeng Zhuang, Chao Chen, Ping WangABSTRACTSurface fabrication is an effective method for functional materials development. This work investigated the use of cellulose-binding domain (CBD) fused detergent lipase for fabrication of easy-cleaning surfaces. As a result, the CBD conjugated Lipase-A (LipA-CBD) demonstrated a multi-layer self-assemble on cotton fabric surface and enhanced hydrophobicity as detected by both scanning electron microscope and water contact angle measurement. Compared to the normal cotton surfaces, such self-assembly bioactive surfaces afforded effective easy-cleaning functionality against both water and lipids based stains with the most significant stain removing ratio as examined through the simulated laundering test. Additionally, this surface assembled LipA-CBD presented good thermal stability with 15 days of half-life detected for 70°C and over 60 days for room temperature. Although there is a gradual decrease in sun irradiation stability and laundering durability, the functionality could be quickly recovered by re-applying the LipA-CBD as the self-assembly coating in the rinsing process. These results presented a green, simple and timesaving method to develop new easy-cleaning cotton fabrics via interfacial self-assembly of biomacromolecules.Graphical abstractGraphical abstract for this article
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