Subjects -> CHEMISTRY (Total: 964 journals)
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CHEMISTRY (692 journals)                  1 2 3 4 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 18)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 33)
ACS Applied Polymer Materials     Hybrid Journal   (Followers: 1)
ACS Catalysis     Hybrid Journal   (Followers: 58)
ACS Chemical Neuroscience     Hybrid Journal   (Followers: 23)
ACS Combinatorial Science     Hybrid Journal   (Followers: 23)
ACS Macro Letters     Hybrid Journal   (Followers: 29)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 48)
ACS Nano     Hybrid Journal   (Followers: 389)
ACS Photonics     Hybrid Journal   (Followers: 17)
ACS Symposium Series     Full-text available via subscription   (Followers: 3)
ACS Synthetic Biology     Hybrid Journal   (Followers: 31)
Acta Chemica Iasi     Open Access   (Followers: 8)
Acta Chimica Slovaca     Open Access   (Followers: 4)
Acta Chimica Slovenica     Open Access   (Followers: 2)
Acta Chromatographica     Full-text available via subscription   (Followers: 9)
Acta Facultatis Medicae Naissensis     Open Access   (Followers: 1)
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 8)
Acta Scientifica Naturalis     Open Access   (Followers: 3)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 9)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 10)
Adsorption Science & Technology     Open Access   (Followers: 8)
Advanced Electronic Materials     Hybrid Journal   (Followers: 1)
Advanced Functional Materials     Hybrid Journal   (Followers: 68)
Advanced Science Focus     Free   (Followers: 6)
Advanced Theory and Simulations     Hybrid Journal   (Followers: 1)
Advanced Therapeutics     Hybrid Journal   (Followers: 1)
Advances in Chemical Engineering and Science     Open Access   (Followers: 89)
Advances in Chemistry     Open Access   (Followers: 30)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 21)
Advances in Drug Research     Full-text available via subscription   (Followers: 26)
Advances in Environmental Chemistry     Open Access   (Followers: 9)
Advances in Enzyme Research     Open Access   (Followers: 11)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 9)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 17)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 11)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 30)
Advances in Nanoparticles     Open Access   (Followers: 19)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Polymer Science     Hybrid Journal   (Followers: 49)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 19)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 7)
Advances in Science and Technology     Full-text available via subscription   (Followers: 14)
Aerosol Science and Engineering     Hybrid Journal  
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 5)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 7)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Al-Kimia : Jurnal Penelitian Sains Kimia     Open Access  
Alchemy : Journal of Chemistry     Open Access   (Followers: 3)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
Alotrop     Open Access  
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 71)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 22)
American Journal of Chemistry     Open Access   (Followers: 35)
American Journal of Plant Physiology     Open Access   (Followers: 13)
American Mineralogist     Hybrid Journal   (Followers: 13)
Anadolu University Journal of Science and Technology A : Applied Sciences and Engineering     Open Access  
Analyst     Full-text available via subscription   (Followers: 37)
Angewandte Chemie     Hybrid Journal   (Followers: 196)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 301)
Annales Universitatis Mariae Curie-Sklodowska, sectio AA – Chemia     Open Access   (Followers: 1)
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 4)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 4)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 8)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 15)
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 2)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 9)
Applied Spectroscopy     Full-text available via subscription   (Followers: 26)
Applied Surface Science     Hybrid Journal   (Followers: 33)
Arabian Journal of Chemistry     Open Access   (Followers: 6)
ARKIVOC     Open Access   (Followers: 1)
Asian Journal of Applied Chemistry Research     Open Access   (Followers: 1)
Asian Journal of Biochemistry     Open Access   (Followers: 3)
Asian Journal of Chemical Sciences     Open Access   (Followers: 1)
Asian Journal of Chemistry and Pharmaceutical Sciences     Open Access   (Followers: 2)
Asian Journal of Physical and Chemical Sciences     Open Access   (Followers: 1)
Atomization and Sprays     Full-text available via subscription   (Followers: 6)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 4)
Avances en Quimica     Open Access  
Biochemical Pharmacology     Hybrid Journal   (Followers: 11)
Biochemistry     Hybrid Journal   (Followers: 411)
Biochemistry Insights     Open Access   (Followers: 7)
Biochemistry Research International     Open Access   (Followers: 7)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 11)
Bioinspired Materials     Open Access   (Followers: 5)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access   (Followers: 3)
Biomacromolecules     Hybrid Journal   (Followers: 25)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 11)
Biomedical Chromatography     Hybrid Journal   (Followers: 6)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 6)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 185)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 89)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 17)
Biosensors     Open Access   (Followers: 3)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 1)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 26)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 4)
Cakra Kimia (Indonesian E-Journal of Applied Chemistry)     Open Access  
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 2)
Canadian Journal of Chemistry     Hybrid Journal   (Followers: 12)
Canadian Mineralogist     Full-text available via subscription   (Followers: 7)
Carbohydrate Research     Hybrid Journal   (Followers: 24)
Carbon     Hybrid Journal   (Followers: 72)
Catalysis for Sustainable Energy     Open Access   (Followers: 10)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 9)
Catalysis Science and Technology     Hybrid Journal   (Followers: 10)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 13)
Cellulose     Hybrid Journal   (Followers: 14)
Cereal Chemistry     Full-text available via subscription   (Followers: 5)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 2)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 22)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 79)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 27)
Chemical Physics Letters : X     Open Access   (Followers: 2)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Hybrid Journal   (Followers: 24)
Chemical Reviews     Hybrid Journal   (Followers: 233)
Chemical Science     Open Access   (Followers: 35)
Chemical Science International Journal     Open Access   (Followers: 1)
Chemical Technology     Open Access   (Followers: 49)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 5)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 56)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 21)
ChemInform     Hybrid Journal   (Followers: 8)
Chemistry     Open Access  
Chemistry & Biodiversity     Hybrid Journal   (Followers: 7)
Chemistry & Biology     Full-text available via subscription   (Followers: 32)
Chemistry & Industry     Full-text available via subscription   (Followers: 8)
Chemistry - A European Journal     Hybrid Journal   (Followers: 195)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 18)
Chemistry Africa : A Journal of the Tunisian Chemical Society     Hybrid Journal  
Chemistry and Materials Research     Open Access   (Followers: 23)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Open Access   (Followers: 3)
Chemistry Letters     Full-text available via subscription   (Followers: 46)
Chemistry of Heterocyclic Compounds     Hybrid Journal   (Followers: 4)
Chemistry of Materials     Hybrid Journal   (Followers: 294)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 10)
Chemistry World     Full-text available via subscription   (Followers: 21)
Chemistry-Didactics-Ecology-Metrology     Open Access   (Followers: 1)
ChemistryOpen     Open Access   (Followers: 1)
ChemistrySelect     Hybrid Journal  
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
ChemNanoMat     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 3)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 15)
Chemosensors     Open Access   (Followers: 1)
ChemPhotoChem     Hybrid Journal  
ChemPhysChem     Hybrid Journal   (Followers: 12)
ChemPlusChem     Hybrid Journal   (Followers: 2)
Chempublish Journal     Open Access   (Followers: 1)
ChemSystemsChem     Hybrid Journal   (Followers: 1)
ChemTexts     Hybrid Journal   (Followers: 1)
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 12)
Chromatographia     Hybrid Journal   (Followers: 22)
Chromatography     Open Access   (Followers: 3)
Chromatography Research International     Open Access   (Followers: 5)
Ciencia     Open Access   (Followers: 1)
Clay Minerals     Hybrid Journal   (Followers: 9)
Cogent Chemistry     Open Access   (Followers: 3)
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 11)
Colloids and Interfaces     Open Access  
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 8)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 4)
Combustion Science and Technology     Hybrid Journal   (Followers: 23)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Communications Chemistry     Open Access   (Followers: 2)
Composite Interfaces     Hybrid Journal   (Followers: 8)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 2)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 2)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 10)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 13)
Computational Chemistry     Open Access   (Followers: 3)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 11)

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Journal Cover
Colloids and Surfaces B: Biointerfaces
Journal Prestige (SJR): 1.071
Citation Impact (citeScore): 4
Number of Followers: 8  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0927-7765 - ISSN (Online) 1873-4367
Published by Elsevier Homepage  [3147 journals]
  • Cationic conjugated polymers for enhancing beneficial bacteria adhesion
           and biofilm formation in gut microbiota
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Pengbo Zhang, Chunling Xu, Xin Zhou, Ruilian Qi, Libing Liu, Fengting Lv, Zhengping Li, Shu WangIt is important to develop efficient therapeutic methods to maintain a healthy balance among gut microbiota by increasing the beneficial bacteria and decreasing the harmful bacteria. In this work, a cationic polythiophene derivative poly(3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrochloride) (PMNT) with quaternary ammonium groups as side chains has been used for efficiently promoting the initial adhesion and biofilm formation of beneficial bacteria in gut microbiota. Upon addition of PMNT, three species of gut microbiota have an increased biofilm formation ability (216.5 % for Escherichia coli (E. coli), 130.7 % for Bifidobacterium infantis (B. infants) and 47.6 % for Enterococcus faecalis (E. faecalis)). As the initial adhesion of bacteria to a surface is an essential step during biofilm formation, PMNT can promote the attachment of bacteria by forming bacteria /PMNT aggregates which possess more cell-to-cell interactions. RNA sequencing results of bacteria within biofilm indicate that the utilization of carbohydrate and glycan is accelerated in the presence of PMNT, leading to enhanced quorum sensing and biofilm formation of E. coli. After forming biofilm, beneficial bacteria have an enhanced resistance to adverse environmental conditions which is significant for maintaining the balance of gut microbiota. Conjugated polymers exhibit a good potential application in modulating the balance of gut microbiota and development of new probiotics drugs.Graphical abstractCationic polythiophene derivative (PMNT) shows the ability to simultaneously promote the initial adhesion and biofilm formation of beneficial bacteria in gut microbiota through increasing cell-to-cell interactions. After forming biofilm, bacteria have an enhanced resistance to adverse environmental conditions which is significant for maintaining the balance of gut microbiota.Graphical abstract for this article
       
  • pH-Triggered geometrical shape switching of a cationic peptide
           nanoparticle for cellular uptake and drug delivery
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Zhongying Gong, Jun Lao, Feng Gao, Weiping Lin, Tao Yu, Baolong Zhou, Jinhua Dong, Hao Liu, Jingkun BaiThe geometry of nanoparticles plays an important role in their performance as drug carriers. However, the pH-triggered geometrical shape switching of a cationic peptide consisting of isoleucine and lysine is seldom reported. In this work, we designed a cationic peptide with acid reactivity that can be loaded with the poorly soluble antitumor drug (doxorubicin (DOX)) to enhance tumor cell uptake and drug delivery. In a weakly acidic environment, a large portion of random coil structures formed, which subsequently led to nanoparticle destruction and rapid DOX release. In vitro studies demonstrated that this cationic peptide exhibits low toxicity to normal cells. The amount of DOX-encapsulating peptide nanoparticles taken up by tumor cells was greater than that taken up by normal cells. Our results indicated that the use of a weakly acidic microenvironment to induce geometric shape switching in drug-loaded peptide nanoparticles should be a promising strategy for antitumor drug delivery.Graphical abstractGraphical abstract for this article
       
  • Lycopene used as Anti-inflammatory Nanodrug for the Treatment of
           Rheumathoid Arthritis: Animal assay, Pharmacokinetics, ABC Transporter and
           Tissue Deposition
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Vivian Meira Moia, Filipe Leal Portilho, Tatiana Almeida Pádua, Luana Barbosa Corrêa, Eduardo Ricci-Junior, Elaine Cruz Rosas, Luiciana Magalhaes Rebelo Alencar, Francisco Savio Mendes Sinfronio, Angelina Sampson, Surtaj Hussain Iram, Frank Alexis, Maria dasGraccedilasMuller de OliveiraHenriques, Ralph Santos-OliveiraLycopene is a carotenoid drug that has demonstrated several properties, including antioxidant and anti-inflammatory activity. The absorption in human body is very low (10–30 % only). In order to increase the bioavailability, lycopene nanoemulsion was formulated and characterized (atomic force microscopy, thermogravimetric analysis dynamic light scattering and differential scanning calorimetry). Also in vitro assay to evaluate the at-binding with MPR1 was performed. Finally, in vivo assay in animals inducted with rheumathoid arthritis were performed. The results showed that the formulated nanolycopene had superior efficacy when compared with the conventional lycopene (not nano-formulated) in inducted animals (rheumatoid arthritis). The results support the use of nanolycopene as an anti-inflammatory agent for rheumatoid arthritis therapy.Graphical abstractGraphical abstract for this article
       
  • Radiolabelling of lipid-based nanocarriers with fluorine-18 for in
           vivo
    tracking by PET
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Surasa Nagachinta, Guillaume Becker, Sylvestre Dammicco, Maria Elisa Serrano, Natacha Leroi, Mohamed Ali Bahri, Alain Plenevaux, Christian Lemaire, Rafael Lopez, André Luxen, Maria de la FuenteOrganic nanoparticles made out of biodegradable and biocompatible materials have attracted increased attention in the therapeutic and diagnostic fields. In this study, we attempted to explore a new radiolabelling chelating free strategy for biodegradable sphingomyelin nanometric emulsions with fluorine-18 (18F), a radioisotope regularly used in clinic. [18F]fluoride was produced by the cyclotron and was incorporated into 4-[18F]fluorobenzamido-N-ethylmaleimide ([18F]FBEM), which was coupled next to the emulsions previously functionalized with a thiol group, via inclusion of either a thiol-PEG-lipid (SH-PEG12-C18), or a peptide-PEG-lipid (Cys-Pro-Ile-Glu-Asp-Arg-Pro-Met-Cys-PEG8-C18) derivative. Radiolabelled emulsions were obtained in a rapid and efficient fashion through facile-conjugated chemistry without the use of organic solvents, and characterized in terms of size, polydispersity, surface charge, pH, and osmolarity. PET imaging and biodistribution studies in BALB/c mice allowed obtaining the pharmacokinetics of the radiolabelled emulsions and determining the clearance pathways. Altogether, we confirmed the potential of this new technique for the radiolabelling of lipid-based drug nanosystems for application in PET imaging diagnosis.Graphical abstractGraphical abstract for this article
       
  • Immobilized lipase in bio-based metal-organic frameworks constructed by
           biomimetic mineralization: A sustainable biocatalyst for biodiesel
           synthesis
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Qing Li, Yingxuan Chen, Shaowei Bai, Xinxin Shao, Lin Jiang, Quanshun LiHerein, thermophilic lipase QLM from Alcaligenes sp. has been successfully immobilized in bio-based metal-organic frameworks (MOFs) through biomimetic mineralization, using zinc acetate and adenine as metal ion and organic ligand, respectively. The morphology and structure of lipase@Bio-MOF was systematically characterized by scanning electron microcopy (SEM), transmission electron microcopy (TEM), powder X-ray diffraction (PXRD) and Fourier transform infrared spectra (FT-IR). The enzyme loading in immobilized enzyme was measured to be 15.9 % by thermogravimetric analysis (TGA). Further, it was demonstrated to possess favorable catalytic activity and stability under high temperature and alkaline conditions and in the presence of metal ions, using the hydrolysis of p-nitrophenyl caprylate as a model. Finally, the immobilized enzyme was successfully applied in the preparation of biodiesel through the trans-esterification of sunflower oil with methanol, obtaining a conversion of>60 % at a high oil/methanol ratio of 8:1. Meanwhile, it showed excellent recyclability during the biodiesel production, and no changes of morphology and crystal structure were observed after being used for 3 cycles. Overall, the immobilized lipase in bio-based MOFs provided an economically and environmentally viable biocatalyst for the synthesis of biodiesel.Graphical abstractGraphical abstract for this article
       
  • Fabrication of morphologically modified strong supramolecular
           nanocomposite antibacterial hydrogels based on sodium deoxycholate with
           inverted optical activity and sustained release
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Susmita Biswas, Udita Chatterjee, Sriparnika Sarkar, Finaz Khan, Debbethi Bera, Madhumita Mukhopadhyay, Soumyabrata Goswami, Sandip Chakrabarti, Susmita DasLow Molecular Weight (LMWG) gelators are small molecules that form supramolecular self-assembly involving physical forces and are highly biocompatible. However, fragility of these physical gels restricts their applicability where gels of higher mechanical strength are required. Herein, we have developed two different types of 2-D carbon nanomaterials viz. graphene oxide (GO) and carbon nanosheet (CNS) embedded sodium deoxycholate (NaDC) hydrogels. XRD, scanning electron microscopy (SEM), rheology and CD studies suggest significant modification of morphological, mechanical, viscoelastic and optical properties of the nanocomposite gels which is ascribed to the presence of the 2D nanotemplates and participation of different surface functionalities of GO and CNS in the gelation process. The overall shear resistance of both the nanocomposite hydrogels upto a shear rate of 300 shears/s−1 and above reveals tremendously improved mechanical stability with respect to the pure gels. The increased shear strength of the GO/NaDC and CNS/NaDC hydrogels is attributed to their 3–4 times broader and longer ribbon like structures in comparison to the fibrous structure of pure gels. The intact ribbon like morphology and greater entanglement impart 10 folds greater viscosity to GO-NaDC hydrogels as compared to better elasticity of CNS-NaDC hydrogels possessing broken ribbon edges. Most interestingly both GO and CNS influence the optical activity of the gels and presence of GO results in inversion of optical activity. The GO-NaDC gels are also found to demonstrate antibacterial activity against E. coli, and S. aureus. Thus, these extraordinarily modified mechanically strong gels have enhanced potential for use in tissue engineering, enantioselective and sustained drug delivery, topical antibiotics and other biomedical applications.Graphical abstractGraphical abstract for this article
       
  • Targeted degradation of anaplastic lymphoma kinase by gold
           nanoparticle-based multi-headed proteolysis targeting chimeras
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Yingming Wang, Lingfei Han, Fulei Liu, Fubai Yang, Xueyang Jiang, Haopeng Sun, Feng Feng, Jingwei Xue, Wenyuan LiuAnaplastic lymphoma kinase (ALK) is a major target in treating non-small-cell lung cancer, and several ALK inhibitors have been developed to antagonize its kinase activity. However, patients treated with inhibitors ultimately develop drug resistance. Therefore, therapies with new mechanisms of action are needed. Proteolysis targeting chimeras (PROTACs) are molecules that comprise a ligand for binding a protein of interest (POI), a connecting linker and a ligand for recruiting E3 ligase, and cause degradation of the target POI. Here, the first multi-headed PROTAC, as a proof of concept, is developed as a gold nanoparticle (GNP)-based drug delivery system for delivering PROTACs to target ALK. Pegylated GNPs loaded with both ceritinib and pomalidomide molecules, termed Cer/Pom-PEG@GNPs, showed good stability in several media. The GNP conjugates potently decreased the levels of ALK fusion proteins in a dose- and time-dependent manner, and specifically inhibited the proliferation of NCI-H2228 cells. In comparison with small molecule PROTACs, the new multi-headed PROTAC promoted the formation of coacervates of POIs/multi-headed PROTAC/E3 ubiquitin ligases, and POI and E3 ubiquitin ligase interacted through multidirectional ligands and a flexible linker, thereby avoiding the need for complicated structure optimization of PROTACs. In conclusion, Cer/Pom-PEG@GNPs can degrade intracellular ALK fusion proteins with minor off-target toxicity and can be applied in patients resistant to ALK inhibitors. As a nano-based drug carrier, Cer/Pom-PEG@GNPs have the potential to enable prolonged circulation and specifically distribute drugs to tumor regions in vivo; thus, further investigation is warranted.Graphical abstractGraphical abstract for this article
       
  • Preparation and application of silica nanoparticles-Ocimum basilicum seeds
           bio-hybrid for the efficient immobilization of invertase enzyme
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Archana Mishra, Jose Savio Melo, Ashish Agrawal, Yogesh Kashyap, Debasis SenFor efficient utilization of immobilized invertase enzyme, a novel bio-hybrid support comprising silica nanoparticles and Ocimum basilicum seed was synthesized. Ocimum basilicum seeds provide a natural fibrouspellicular structure which acts as template for assembly of silica nanoparticles. Bio-hybrids of two different morphologies have been obtained by changing the physico-chemical conditions of the assembly process. Developed bio-hybrids were characterized through small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), Synchrotron radiation based X-ray micro-computed tomography (SRμCT), Brunauer–Emmett–Teller (BET) and Fourier transform infrared spectroscopy (FTIR). Incorporation of the nanoparticles results to a fourfold increase in the available surface area of the seeds which is one of the important criteria for an immobilizing support. Synthesized bio-hybrids were used for the immobilization of commercially applicable invertase enzyme and efficient loading of enzyme was realized. Enzyme immobilized bio-hybrids could be easily separated out and reused up to eight times with 82 % retention of enzyme activity. Present work suggests that the unique features of the bio-hybrid make it suitable candidate for immobilization of enzymes in general.Graphical abstractLoading of silica NP on fibrous structure of seed and its application for immobilization of invertase enzyme.Graphical abstract for this article
       
  • Utilization of High throughput microcrystalline cellulose decorated silver
           nanoparticles as an eco-nematicide on root-knot nematodes
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Moustafa M.G. Fouda, Gohar I.M. A., Amira E.M. Hanfy, Sarah I. Othman, Amera F. Zaitoun, Nader R. Abdelsalam, Ahmed A. Allam, Osama M. Morsy, Mehrez E. El-NaggarThe present study aimed to evaluate the influence of high throughput microcrystalline cellulose embedded silver nanoparticles (Ag-NPs), as an alternative eco-nematicide on Root-knot nematode (Meliodogyne incognita), which deem the main reason toward the loss of more than 20% in crops worldwide. In this work, Ag-NPs was prepared in very high concentration. Ag-NPs prepared using such technique has many advantages such as: absence of organic or solvents, scaling up thru using high concentration of silver precursor and utilization of environmentally benign polymer; Microcrystalline Cellulose (MCC). At the beginning, the bulk Ag-NPs colloidal solution is diluted to 5, 10, 15, 20, 25, 30, 40, 50, 60, 75, 80 and 100 ppm. Then, heavily galled roots of annual seed-propagated weed, Solanum nigrum L. family Solanaceae were selected to identify the Meloidogyne species and followed by treatment with the previously Ag-NPs concentrations. Results obtained after 24 h incubation, showed the highest mortality (M%) (40.36 ± 1.15%) which was achieved by means of 20 ppm of Ag-NPs compared with the highest concentration of Ag-NPs; 100 ppm (42.85 ± 3.51%). It was obviously noticed that, by increasing the concentration of Ag-NPs, M % decreased. On the other hand, after 48 h, 30 ppm Ag-NPs showed the highest M%; (52.82 ± 0.57%), while, after 72 h of treatments, the M% reached 95.53 ± 0.57% using 40 ppm Ag-NPs, then decreased to 66.67 ± 2.00% using 100 ppm Ag-NPs. All previous finding affirms the effectiveness of lower concentrations of Ag-NPs compared with the highest one, after 72 h. In conclusion, Ag-NPs could be successfully used as eco-nematicide for Root-knot nematodes; Meloidogyne incognita with a recommended dose of 20−40 ppm that is acquired higher M% and caused many aberrations during the different growth stages.Graphical abstractGraphical abstract for this article
       
  • Performance of PEGylated chitosan and poly (L-lactic
           acid-co-ε-caprolactone) bilayer vascular grafts in a canine femoral
           artery model
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Anlin Yin, Weihua Zhuang, Gongyan Liu, Xiaorong Lan, Zhonglan Tang, Yan Deng, Yunbing WangThe fabrication of a functional small-diameter vascular graft with good biocompatibility, in particular hemocompatibility, has become an urgent clinical necessity. We fabricated a native bilayer, small-diameter vascular graft using PEGylated chitosan (PEG-CS) and poly (L-lactic acid-co-ε-caprolactone; PLCL). To stabilize the inner layer, a PEG-CS blend with PLCL at ratio of 1:6 was casted on a round metal bar by a drip feed, and the outer layer, a PLCL blend with water-soluble PEG that acted as a sacrificial part to enhance pore size, was fabricated by electrospinning. The results showed excellent hemocompatibility and strong mechanical properties. In vitro, the degradation of the graft was evaluated by measuring the graft structure, mass loss rate, and changes in molecular weight. The results indicated that the graft had adequate support for the regeneration of blood vessels before collapse. An in vivo study was performed in a canine femoral artery model for up to 24 weeks, which demonstrated that the PEGylated bilayer grafts possessed excellent structural integrity, high compatibility with blood, good endothelial cell (EC) and smooth muscle cell (SMC) growth, and high expression levels of angiogenesis-related proteins, features that are highly similar to autologous blood vessels. Moreover, the results showed almost negligible calcification within 24 weeks. These findings confirm that the bilayer graft mimics native cells, thereby effectively improving vascular remodeling.Graphical abstractGraphical abstract for this article
       
  • pH-responsive cationic liposome for endosomal escape mediated drug
           delivery
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Sagar Rayamajhi, Jessica Marchitto, Tuyen Duong Thanh Nguyen, Ramesh Marasini, Christian Celia, Santosh AryalEndosomal degradation of the nanoparticle is one of the major biological barriers associated with the drug delivery system. Nanoparticles are internalized in the cell via different endocytosis pathways, where they are first delivered to early endosomes which mature to the late endosome and to the lysosome. During this journey, NP encounters a harsh chemical environment resulting in the degradation of NP and its content. This process is collectively called as intracellular defenses against foreign materials. Therefore, to avoid this degradative fate, the endosomal escape technique has been explored following membrane fusion or membrane destabilization mechanisms. However, these methods are limited to the application due to non-specific membrane fusion. To overcome this limitation, we have designed pH-responsive liposome made up of 3ß-[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride (DC-liposome) in which the cationic nitrogen of the ammonium moiety occupies only ∼2.5 % of the molecule. Such a small percentage of the cationic moiety is sufficient enough to exhibit pH-responsive properties while maintaining the biocompatibility of the DC-liposome. DC-liposome showed pH-dependent cationic properties due to the protonation of DC-moiety at acidic pH. The fluorescence-based experiment confirmed pH-dependent fusogenic properties of DC-liposome. Furthermore, the endosomal colocalization study revealed higher localization of DC-liposome in the early endosome compared to that of the late endosome, suggesting possible endosomal escape. Elevated cationic and fusogenic properties of DC-liposome at acidic pH can mediate membrane fusion with anionic endosomal membrane via electrostatic interaction, thereby causing endosomal escape. Moreover, doxorubicin-loaded DC-liposome showed higher cytotoxicity than that of free doxorubicin further supporting our clam of endosomal escape. These findings suggest the potential of DC-liposome to break the endosomal barriers to enhance the therapeutic efficacy thereby guiding us in design consideration in the field of stimuli-responsive delivery agents.Graphical abstractGraphical abstract for this article
       
  • Interaction of nitrofurantoin with lipid langmuir monolayers as cellular
           membrane models distinguished with tensiometry and infrared spectroscopy
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): André C. Machado, Luciano CaseliKnowing how a drug interacts with cell membranes is important to understand and predict its effects at the molecular level. Therefore, we aimed to study the interaction of nitrofurantoin (NFT), a compound with potential antibiotic and antitumor properties, with lipidic biological interfaces using Langmuir monolayers. We employed the phospholipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS), which were spread on the surface of water to form Langmuir films, to investigate the membrane-drug interactions. The interaction of the drug with the lipid monolayers was evaluated by using surface pressure-area isotherms, surface pressure-time kinetic curves, Brewster angle microscopy (BAM), and polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS). Nitrofurantoin shifted the isotherms to lower DPPC molecular areas, indicating monolayer condensation, and to higher DPPS molecular areas, indicating monolayer expansion. Meanwhile, BAM images showed the appearance of interfacial aggregates for DPPS, but not for DPPC, in the presence of NFT. PM-IRRAS spectra showed that bands related to methylene stretches changed their relative intensities and maximum position related to their asymmetric and symmetric modes for both lipids. This suggested an alteration of the monolayer packing degree and the conformational order of the lipid alkyl chains, which were related to an increase in configurational order for DPPS, but disorder for DPPC. In conclusion, NFT caused distinctive changes in the thermodynamic, morphological, and structural properties of DPPC and DPPS monolayers, which may be associated with its bioactivity in cellular membranes and other lipidic interfaces of pharmaceutical interest.Graphical abstractGraphical abstract for this article
       
  • Mechanical and release behaviour of theophylline from matrix tablets
           containing psyllium powder in combination with grewia polysaccharides
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): E.I. Nep, N. Kaur, S. Shaboun, A.O. Adebisi, A.M. Smith, B.R. Conway, K. Asare-AddoThis study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium. Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug. The true, bulk and tapped densities, Carr’s compressibility index of the powders and binary composites were determined before tablet compression. Tablet properties (hardness, porosity, and drug release from the matrices) were investigated. The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8). The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N). The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former. The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs. The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n> 0.45. When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.Graphical abstractGraphical abstract for this article
       
  • Preparation and application of PGMA-DVB microspheres via
           surface-modification with quaternary and phenylboronic acid moiety
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Long Pang, Tingting Xue, Hailin Cong, Youqing Shen, Bing YuPorous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 ± 0.24 μm in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.Graphical abstractGraphical abstract for this article
       
  • An activatable liposomal fluorescence probe based on fluorescence
           resonance energy transfer and aggregation induced emission effect for
           sensitive tumor imaging
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Yuqiong Xia, Chunzhong Xu, Xianghan Zhang, Jingkai Gao, Yankun Wu, Cairu Li, Zhongliang WangLiposomes are of great interest and importance in tumor imaging, since they can greatly improve the imaging sensitivity and specificity by increasing the accumulation of contrast agents. Still, most liposome-based probes have high background signals during blood circulation, which limits enhancement of S/B ratio and tumor imaging sensitivity. To enhance the S/B ratio of tumor imaging, we construct a fluorescence resonance energy transfer (FRET) and aggregation induced emission (AIE) based liposomal fluorescence probe TPE/BHQ-lipo with excellent FRET effect (99 %) and great fluorescence enhancement upon liposome rupture (120-fold) as well as efficient fluorescence recovery in tumor cell imaging. Finally, we used the TPE/BHQ-lipo to image 4T1 tumor upon intravenous injection of liposomes and the group showed enhanced signal to background ratio of 4.1, compared to 1.8 from control AIE-based liposomal group (TPE-lipo). Our work offers an excellent FRET and AIE-based liposomal probe for high-sensitive tumor imaging.Graphical abstractGraphical abstract for this article
       
  • Biofilm formation of clinically important microorganisms on 2D and 3D poly
           (methyl methacrylate) substrates: A surface-enhanced Raman scattering
           study
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Seda Keleştemur, Zehra Çobandede, Mustafa ÇulhaClinically relevant microorganisms threaten patient’s health often through biofilm formation on polymeric medical devices and implants. Poly (methyl methacrylate) is a commonly used polymer in medical implants and dental devices. In this study, biofilm characteristics of model microorganisms, Pseudomonas aeruginosa, Staphylococcus epidermidis and Candida albicans, were investigated at molecular level on 2-dimensional (2D) and 3-dimensional (3D) PMMA substrates to understand the influence of surface structures on biofilm formation and also to demonstrate the discrimination of microorganisms according to their metabolic activities by utilizing surface-enhanced Raman scattering (SERS). It was found that the fibrous 3D structure enhanced the assembly of microorganisms and enriched the biofilm structure while smooth polymeric surface decreased the biofilm formation rate and variety of biofilm content. Among the studied microorganisms, Pseudomonas aeruginosa and Candida albicans had a higher tendency to form biofilm on both 2D and 3D PMMA substrates. Although Staphlylococcus epidermidis showed slow adaption on PMMA surfaces, the 3D porous surfaces increased its biofilm formation rate significantly compared to 2D surface.Graphical abstractGraphical abstract for this article
       
  • Preparation and characterization of copper and zinc adsorbed
           cetylpyridinium and N-lauroylsarcosinate intercalated montmorillonites and
           their antibacterial activity
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Günseli Özdemir, Saadet YaparThe main objective of the present study was the preparation and characterization of new cationic/anionic surfactants and Cu2+/Zn2+ modified montmorillonites and the evaluation of their potential applicability as antibacterial agents for topical applications. To evaluate the antibacterial activity of Cu2+ and Zn2+ by synergistic effect, as well as to reduce the well-known toxicity of these metal cations; cetylpyridinium (CP) and N-lauroylsarcosinate (SR) intercalated montmorillonite (Mt-CP-SR) was used as the host material. In addition to their role to capture the metal cations and inhibit their release in any contact medium, these surfactants also increase the efficacy of the material due to their antibacterial properties. The effect of surfactant loading on the adsorption behavior of the metal cations onto the Mt-CP was investigated using SR in two different concentrations, namely 0.7 and 1.0 CEC of sodium montmorillonite (Mt-Na). The samples prepared were characterized using SEM, ATR-FTIR, zeta potential, and XRD analyses and they were subjected to antibacterial tests using the “Standard Method Under Dynamic Contact Conditions” on the Gram positive S. aureus, and Gram negative E. coli. As confirmed with desorption and characterization studies, the addition of Cu2+/Zn2+ onto the Mt-CP-SR yielded double adsorbed amounts compared to that of the Mt-CP, which indicated that Cu2+/Zn2+ bound to SR− interacted with the Mt surface. In contrary of Zn2+ caused no considerable change in the antibacterial effect of the host, Cu2+ addition enhanced the antibacterial activity. The produced antibacterial agents have the potential use in dyes, polymer composites, personal care products, and topical medicinal applications.Graphical abstractGraphical abstract for this article
       
  • Intracellular pH-propelled assembly of smart carbon nanodots and selective
           photothermal therapy for cancer cells
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Xue Zhang, Yanting Shen, Shuping Xu, Jing Yue, Qiong Guo, Dianshuai Huang, Bai Yang, Wei Shi, Chongyang Liang, Weiqing XuA kind of smart carbon nanodots (CNDs) with the pH response feature was prepared by the one-pot hydrothermal treatment of citric acid and dicyandiamide, which was used for the differentiation of cancer/normal cells and the selective photothermal therapy (PTT) of cancer cells. When the smart CNDs were cultured with cells, they were highly internalized in the lysosomes of cells. Since the small-sized CNDs (about 5 nm) tends to form aggregation (as large as about 20 nm or even larger) under an acid condition (pH = 4.7) due to the electrostatic attraction produced by the surface protonation, relatively severer aggregation of the CNDs were observed in liver cancer cells (HepG2 cells) relative to normal ones (LO2 cells) due to a relative lower pH in the lysosomes of HepG2 cells, which endows them a new strong absorption band at longer wavelengths (450−900 nm) and a higher photothermal conversion efficiency (42.13 %), benefiting to differentiated PTT. The flow cytometric data indicates strong photothermal ablation (8 min, 509.6 mW/cm2) for cancer cells with the assistance of these smart CNDs achieves 82 % death rate of cancer cells, while much less damage is observed on the normal cells (6.35 %). To the best of our knowledge, this is the first report about CNDs for selective PTT owing to their intrinsic property without the aid of any other targeting ligands. These smart CNDs are also available for other acid-responsive sensing systems, and this study inspires us in the synthesis of near-infrared featured carbon materials.Graphical abstractGraphical abstract for this article
       
  • Supramolecular functionalization of carbon nano-onions with hyaluronic
           acid-phospholipid conjugates for selective targeting of cancer cells
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Marta d’Amora, Adalberto Camisasca, Alice Boarino, Silvia Giordani, Silvia ArpiccoCarbon nano-onions (CNOs) are promising materials for biomedical applications due to their low cytotoxicity and excellent biocompatibility. Supramolecular functionalization with biocompatible polymers is an effective strategy to develop engineered drug carriers for targeted delivery applications. In this study, we report the use of a hyaluronic acid-phospholipid (HA-DMPE) conjugate to target CD44 overexpressing cancer cells, while enhancing solubility of the nanoconstruct. Non-covalently functionalized CNOs with HA-DMPE show excellent in vitro cell viability in human breast carcinoma cells overexpressing CD44 and are uptaken to a greater extent compared to human ovarian carcinoma cells with an undetectable amount of CD44. In addition, they possess high in vivo biocompatibility in zebrafish (Danio Rerio) during the different stages of development and they prevalently localize in the digestive tract of the zebrafish larvae.Graphical abstractGraphical abstract for this article
       
  • Design of heterostructured hybrids comprising ultrathin 2D bismuth
           tungstate nanosheets reinforced by chloramphenicol imprinted polymers used
           as biomimetic interfaces for mass-sensitive detection
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Ayesha Shaheen, Ayesha Taj, Peter A. Liberzeit, Adnan Mujahid, Sadaf Hameed, Haijun Yu, Arshad Mahmood, Thomas J. Webster, M. Hamid Rashidd, Waheed S. Khan, Sadia Z. BajwaCombining nanomaterials in varying morphology and functionalities gives rise to a new class of composite materials leading to innovative applications. In this study, we designed a heterostructured hybrid material consisting of two-dimensional bismuth nanosheets augmented by molecularly imprinted networks. Antibiotic overuse is now one of the main concerns in health management, and their monitoring is highly desirable but challenging. So, for this purpose, the resulting composite interface was used as a transducer for quartz crystal microbalances. The main objective was to develop highly selective mass-sensitive sensor for chloramphenicol. Morphological investigation revealed the presence of ultrathin, square shaped nanosheets, 2−3 nm in height and further supplemented by imprinted polymers. Sensor responses are described as the decrease in the frequency of microbalances owing to chloramphenicol re-binding in the templated cavities, yielding a detection limit down to 0.74 μM. This sensor demonstrated a 100 % specific detection of chloramphenicol over its interfering and structural analogs (clindamycin, thiamphenicol, and florfenicol). This composite interface offers the advantage of selective binding and excellent sensitivity due to special heterostructured morphology, in addition to benefits of robustness and online monitoring. The results suggest that such composite-based sensors can be favorable platforms, especially for commercial prospects, to obtain selective detection of other biomolecules of clinical importance.Graphical abstractGraphical abstract for this article
       
  • Controlled release and antioxidant activity of chitosan and
           β-lactoglobulin complex nanoparticles loaded with epigallocatechin
           gallate
    • Abstract: Publication date: Available online 16 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Wenzhong Dai, Chengcheng Ruan, Yue Sun, Xueling Gao, Jin LiangIn order to investigate the influence of different embedding methods on the properties and function of polyphenols, the Epigallocatechin gallate (EGCG) loaded chitosan nanoparticles prepared with or without β-lactoglobulin (β-Lg) were obtained by ionic cross linking method. The average particle sizes of EGCG loaded chitosan nanoparticles (EGCG-CS NPs) decreased from 190 nm to 157 nm after adding with β-Lg, whereas the encapsulation efficiency (EE) increased from 59.79% to 76.29%. The results of transmission electron microscopy (TEM) showed that the obtained nanoparticles had obvious core-shell structure. The results of simulated gastrointestinal digestion showed that the release rate of EGCG in CS/β-Lg NPs was much lower than that of CS-NPs. Compared with free EGCG, the DPPH and FRAP assay showed that EGCG-CS NPs and EGCG-CS/β-Lg NPs had slow-controlled antioxidant activity. Meanwhile, the study of cellular antioxidant activity (CAA) showed that the EC50 values of EGCG-CS NPs and EGCG-CS/β-Lg NPs were decreased by 8.56% and 18.35%, respectively.Graphical abstractGraphical abstract for this article
       
  • Tyrosinase-mediated dopamine polymerization modified magnetic alginate
           beads for dual-enzymes encapsulation: Preparation, performance and
           application
    • Abstract: Publication date: Available online 15 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Hao Zhang, Min Lu, Hui Jiang, Zhao-Yu Wu, Dong-Dong Zhou, De-Qiang Li, Feng-Qing YangIn this study, a simple and efficient method to obtain entrapment of mixtures of double enzymes is developed. As a proof of principle, double enzymes (tyrosinase (TYR) and β-glucosidase (β-Glu)) were co-immobilized in magnetic alginate-polydopamine (PDA) beads using in situ TYR-mediated dopamine polymerization and internal setting strategy-mediated magnetic alginate-PDA gelation. The leakage of enzymes from the magnetic alginate beads was significantly reduced by exploiting the double network cross-linking of alginate and PDA, which was induced by the D-(+)-Gluconic acid δ-lactone (GDL) and TYR, respectively. The physicochemical properties of the prepared magnetic alginate beads were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis. After that, the enzymatic reaction conditions and the performance of the entrapped TYR and β-Glu, such as enzyme kinetics and inhibition kinetics, were investigated. The Michaelis-Menten constants (Km) of the entrapped TYR and β-Glu were determined as 2.72 and 3.45 mM, respectively. The half-maximal inhibitory concentrations (IC50) of kojic acid and castanospermine for the entrapped TYR and β-Glu were determined as 13.04 and 56.23 μM, respectively. Finally, the entrapped double enzymes magnetic alginate beads were successfully applied to evaluate the inhibitory potency of six kinds of tea polyphenols extracts. Black tea and white tea showed high inhibition activity against TYR were (36.14 ± 1.43)% and (36.76 ± 2.35)%, respectively, while the black tea and dark tea showed high inhibition activity against β-Glu were (37.89 ± 6.70)% and (21.28 ± 4.68)%, respectively.Graphical Graphical abstract for this article
       
  • Glucose sensor based on Pd nanosheets deposited on Cu/Cu2O nanocomposites
           by galvanic replacement
    • Abstract: Publication date: Available online 15 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Li Tang, Ke Huan, Dongmei Deng, Long Han, Zhigang Zeng, Liqiang LuoA novel non-enzymatic glucose sensor was established by galvanic replacement synthesis of Pd nanosheets (PdNS) on Cu/Cu2O nanocomposites using fluorine-doped tin oxide (FTO) substrates. To prepare PdNS-Cu/Cu2O/FTO electrode, Cu/Cu2O nanocomposites were firstly deposited on FTO electrode through potential oscillating, and then Pd nanosheets were synthesized on Cu/Cu2O nanocomposites by galvanic replacement reduction. X-ray diffraction, scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy were applied to investigate the morphology and composition of PdNS-Cu/Cu2O nanohybrids. The synthesized PdNS-Cu/Cu2O exhibited great electrochemical activity toward glucose oxidation. Under optimized conditions, the proposed glucose sensor displayed a wide linear range from 0.5 μM to 2600 μM with detection limit of 0.1 μM. In addition, the proposed electrode was used to determine glucose in true blood specimen, indicating potential application for routine glucose analysis.Graphical abstractGraphical abstract for this article
       
  • Development of Micropatterning Polyimide Films for Enhanced Antifouling
           and Antibacterial Properties
    • Abstract: Publication date: Available online 15 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Emma A. Cuello, Lucinda E. Mulko, Cesar A. Barbero, Diego F. Acevedo, Edith I. YslasA commercial biomedical Polyimide (PI) film was topographically and chemically modified by generating micrometric periodic arrays of lines using Direct Laser Interference Patterning (DLIP) in order to improve antifouling and antibacterial properties. DLIP patterning was performed with periods from 1 μm to 10 μm. The physical modification of the surface was characterized by SEM, AFM and contact angle measurements and, the chemical composition of the ablated surfaces was analyzed by ATR-IR and XPS spectroscopies. The antibacterial effects were evaluated through the effect on Pseudomonas aeruginosa colonies growth on the LB (Luria Bertani) broth. The results showed that the laser treatment change the topography and as a consequence the chemistry surface, also that the microstructured surfaces with periods below 2 μm, exhibited a significant bacterial (P. aeruginosa) adhesion decrease compared with non-structured surfaces or with surfaces with periods higher than 2 μm. The results suggest that periodic topography only confer antifouling properties and reduction of the biofilm formation when the microstructure presents periods ranging from 1 μm to 2 μm. On the other hand, the topography that confer strong antifouling superficial properties persists at long incubation times. In that way, polymer applications in the biosciences field can be improved by a surface topography modification using a simple, single-step laser-assisted ablation method.Graphical abstractGraphical abstract for this article
       
  • An efficient two-step preparation of photocrosslinked gelatin microspheres
           as cell carriers to support MC3T3-E1 cells osteogenic performance
    • Abstract: Publication date: Available online 15 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ji Jiang, Amin Liu, Chunlan Chen, Jiajia Tang, Hongjun Fan, Jing Sun, Hongsong FanGelatin microspheres have been commonly used in tissue engineering, but their application is often limited by the uncontrollability and potential cytotoxicity of traditional chemical cross-linking method. Methylacrylamide modification and photocrosslinking provide a controllable and cytocompatible cross-linking method for gelatin hydrogels, however, microspheres fabricated by this single photopolymerization process is uncontrollable. In this study, we show that increasing the gelling ability of gelatin methacrylamide (GMA) at low temperatures is vital to prepare photocrosslinked gelatin microspheres, which in turn improves the controllability and compatibility of conventional chemical cross-linking methods. We detailed characterized the rheological performance with varying temperature and demonstrated that the gelling capability of GMA could be improved by increasing GMA solution concentration and reducing methacrylate substitution. The physicochemical properties of the photocrosslinked microspheres can be modulated via methacrylamide modification, as evidenced by the positive correlation between the physicochemical optimization of the hydrogel bulk and the degree of methacrylate substitution. Next, we successfully fabricated GMA spheres by a two-step process of low-temperature gelation followed by photopolymerization crosslinking. Finally, we show that the microcarriers exhibited favorable supporting for MC3T3-E1 cell proliferation, spreading, and osteogenic differentiation. This study provided a controllable and cytocompatible photocrosslinking procedure for GMA microspheres with broad application prospects, of course, not limited to cell microcarriers.Graphical Graphical abstract for this article
       
  • In vitro and in vivo biocompatibility and bio-tribological properties of
           the Calcium/amorphous-C composite films for bone tissue engineering
           application
    • Abstract: Publication date: Available online 11 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Dongguang Liu, Haoran Ma, Yan Liang, Liang zhengABSTRACTCarbon-and diamond-like-carbon coated Ti alloys hold great promise for tissue engineering applications. Unfortunately, their strong intrinsic stress leads to the adhesion failure of the films. Herein, a series of a-C films with different Ca content were prepared on Ti6Al4V via co-sputtering deposition technology. Homogeneous spherical Ca nanoclusters, with an inner diameter of 2-6 nm, were formed in an amorphous carbon matrix. The addition of Ca induced indistinctive variation in either phase composition or topography. However, the introduction of Ca not only improved the mechanical properties of a-C film but also significantly strengthened its adhesion to osteoblasts. The bio-tribological properties of Ca/a-C films were also assessed using a tribometer in FBS solution. The Ca/a-C films exhibited a low friction coefficient of 0.083 and a low wear rate of 1.02-1.24×10-6  mm3/Nm. The low coefficient of friction (COF) of the Ca/a-C films indicates their superior mechanical properties, making them the promising target of nanocomposite films used in bio-tribological applications. Well-stretched cells and the developed actin filaments were distinctly observed on the Ca/a-C films in the osteoblast cell adhesion experiments. In addition, the Ca/a-C films promoted cell proliferation and showed high cell viability. After being implanted for 4 weeks, the Ca/a-C implant material still adhered well to the muscle tissue, without inducing hyperergic or inflammatory reactions. Collectively, our results suggest that the Ca/a-C film is an ideal mounting material for bone tissue engineering.Graphical abstractGraphical abstract for this article
       
  • Mechanical and microstructural insights of Vibrio cholerae and Escherichia
           coli dual-species biofilm at the air-liquid interface
    • Abstract: Publication date: Available online 11 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Clémence Abriat, Kyle Enriquez, Nick Virgilio, Lynette Cegelski, Gerald G. Fuller, France Daigle, Marie-Claude HeuzeyBiofilm is the dominant microbial form found in nature, in which bacterial species are embedded in a self-produced extracellular matrix (ECM). These complex microbial communities are responsible for several infections when they involve multispecies pathogenic bacteria. In previous studies, interfacial rheology proved to be a unique quantitative technique to follow in real-time the biofilm formation at the air-liquid interface.In this work, we studied a model system composed of two bacteria pathogenic capable of forming a pellicle biofilm, V. cholerae and E. coli. We used an integrated approach by combining a real-time quantitative analysis of the biofilm rheological properties, with the investigation of major matrix components and the pellicle microstructure. The results highlight the competition for the interface between the two species, driven by the biofilm formation growth rate. In the dual-species biofilm, the viscoelastic properties were dominated by V. cholera, which formed a mature biofilm 18 hours faster than E. coli. The microstructure of the dual-species biofilm revealed a similar morphology to V. cholerae alone when both bacteria were initially added at the same amount. The analysis of some major ECM components showed that E. coli was not able to produce curli in the presence of V. cholerae, unless enough time was given for E. coli to colonize the air-liquid interface first. E. coli secreted phosphoethanolamine (pEtN) cellulose in the dual-species biofilm, but did not form a filamentous structure. Our pathogenic model system demonstrated the importance of the biofilm growth rate for multispecies biofilm composition at the air-liquid interface.Graphical abstractGraphical abstract for this article
       
  • Hyaluronic acid and Vitamin E polyethylene glycol succinate functionalized
           gold-core silica shell nanorods for cancer targeted photothermal therapy
    • Abstract: Publication date: Available online 9 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Telma A. Jacinto, Carolina F. Rodrigues, André F. Moreira, Sónia P. Miguel, Elisabete C. Costa, Paula Ferreira, Ilídio J. CorreiaGold-core mesoporous silica shell (AuMSS) nanorods unique physicochemical properties makes them versatile and promising nanomedicines for cancer photothermal therapy. Nevertheless, these nanomaterials present a reduced half-life in the blood and poor specificity towards the tumor tissue. Herein, D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) and Hyaluronic Acid (HA) were combined for the first time to improve the AuMSS nanorods biological performance. The obtained results revealed that AuMSS surface functionalization induced the surface charge neutralization, from -28 ± 10 mV to -3 ± 5 mV and -10 ± 4 mV for AuMSS-TPGS-HA (1:1) and (4:1) formulations, without impacting on nanomaterials’ photothermal capacity. Moreover, the AuMSS functionalization improved the nanomaterials hemocompatibility and selectivity towards the cancer cells, particularly in the AuMSS-TPGS-HA (4:1) formulation. Furthermore, both formulations were able to mediate an on-demand photothermal effect, that induced the HeLa cancer cells death, confirming its potential for being applied as targeted multifunctional theragnostic nanomedicines.Graphical abstractGraphical abstract for this article
       
  • Random Array of Inorganic Nanoparticles on Polymer Surface for
           Anti-Biofouling Property Through Cost-Effective and High-Performance
           Dip-Coating
    • Abstract: Publication date: Available online 9 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Hyoung-Jun Kim, Do-Gak Jeung, Jae-Min OhAnti-biofouling treatment is required in various fields such as biomedical application, construction, civil engineering, and so on. Currently available techniques such as lithography and replica methods have several limitations in application and accessibility. We introduced a simple, biocompatible, and cost-effective anti-biofouling dip-coating method with polyurethane-inorganic (anisotropic montmorillonite and spherical TiO2) hybrid coating agent. Layer thickness of coating was as thin as 5 µm. It was cross-confirmed with thickness gauge and cross-section scanning electron microscopy. Through atomic force microscopy, inorganic nanoparticles were observed to be randomly arrayed with particles partially embedded in the polyurethane network. The calculated surface roughness of inorganic-polyurethane hybrid coating was five times larger than the neat substrate film and three times larger than coating without inorganic nanoparticles. Surface energy of the inorganic-polyurethane film decreased with increasing surface roughness as random pattern of inorganic particle reduced van der Waals interaction. Biofouling efficacy was evaluated by mucin adsorption and consecutive alcian blue assay. Results showed that coated film decreased biofouling 81% compared to bare film.Graphical abstractGraphical abstract for this article
       
  • Insulin-Copper Quantum Clusters Preparation and Receptor Targeted
           Bioimaging
    • Abstract: Publication date: Available online 9 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Pawandeep Kaur, Sunidhi Sharma, Satabdi Datta Choudhury, Deepika Singh, Shreya Sharma, Kundlik Gadhave, Neha Garg, Diptiman ChoudhuryProtein embedded fluorescence quantum clusters (QCs) have received a great amount of interest among the researchers because of their high aqueous solubility, stability, cost efficiency, and target specificity. Considerable advancement has happened in making functional quantum clusters with target specificity. This work reports the simple synthesis of insulin protected copper quantum clusters (ICuQCs) and its receptor-targeted bioimaging applications. The preparation of copper quantum clusters (CuQCs) was done simply by one-pot synthesis method by changing the pH of the insulin protein firstly to 10.5 basic pH than physiological pH. At physiological pH, the mixture incubated in oven 37 ⁰C at 240 rpm has been developed to process initially polydisperse, non-fluorescent, and unstable CuDs into monodispersed (∼2-3 nm), highly fluorescent, and extremely stable ICuQCs in the same phase (aqueous) using insulin as protein. HRTEM image show uniform distribution of CuDs within the protein matrix. Metal ion binding site prediction and docking server (MIB) results show that chain B of insulin contains 3 templates contains 5 amino acid residues which bind with Cu2+ metal ion. Groove 1 contains GLY8 and HIS10 bind has the highest binding potential towards Cu metal ions. The methodology adopted in this study should largely contribute to the practical applications of this new class of QCs. In view of the protein protection, coupled with direct synthesis and easy functionalization, this hybrid QC-protein system is expected to have numerous optical and bioimaging applications in the future.Graphical abstractGraphical abstract for this article
       
  • Long-Lasting anti-bacterial activity and bacteriostatic mechanism of tea
           tree oil adsorbed on the amino-functionalized mesoporous silica-coated by
           PAA
    • Abstract: Publication date: Available online 9 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Fan Gao, Hongjun Zhou, Zhichuan Shen, Guowei Zhu, Li Hao, Huayao Chen, Hua Xu, Xinhua ZhouTea tree oil (TTO) is an efficient natural antibacterial agent. However, the bacteriostatic effect of TTO does not prevail for a long period because of the volatile nature of the oil. Therefore, a novel sustained-release formulation of TTO should be developed for improving the applicability of TTO. Herein, the mesoporous silica was selected for constructing a carrier for TTO. Mesoporous silica is non-toxic, easy to modify and exhibited an adjustable pore size. First, the mesoporous silica was modified by an aminated silane coupling agent (NH2-MCM-41). Then, the polyacrylic acid (PAA) was bonded by electrostatic bonds (PAA-NH2-MCM-41), which imparted the sustained-release effect in the TTO, supported in the mesoporous silica channel (TTO/PAA-NH2-MCM-41). The prepared bacteriostatic agent can achieve long-term sustained-release properties. At room temperature (26 ℃), the release rate of TTO after 11 h release reached 50%. However, the release rate of TTO from TTO/PAA-NH2-MCM-8 reached only 42% after 24 h. Furthermore, the sustained release behavior of TTO/PAA-NH2-MCM-41 was consistent with the Korsmeyer-Peppas kinetic model. Compared to TTO, TTO/PAA-NH2-MCM-41 exhibited a stable and sustained bacteriostatic effect even after 50 days in a natural environment. The minimum inhibitory concentration (MIC) value of the TTO/PAA-NH2-MCM-41 against Escherichia coli (E. coli) was 0.37∼0.44 mg/mL. TTO altered the cell morphology of E. coli and broke the integrity of the cell membrane, leading to cell death.Graphical abstractGraphical abstract for this article
       
  • ANALYSIS OF THE STRUCTURE-FUNCTION RELATIONSHIP OF ALPHA AMYLASE COMPLEXED
           WITH POLYACRYLIC ACID
    • Abstract: Publication date: Available online 9 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): María C. Porfiri, Natasha Melnichuk, Mauricio J. Braia, César Brinatti, Watson Loh, Diana RomaniniAlpha-amylase is frequently used in technologies that require its immobilization, stabilization or encapsulation. Polyacrylic acid is a very suitable polymer for these purposes because it can bind to enzymes and then be released under certain conditions without altering the functional capacity of enzymes. The consequences produced by polyacrylic acid on alpha-amylase structure and function have been investigated through various techniques. Calorimetric measurements allowed examining the nature of the binding reaction, stoichiometry and affinity, while spectroscopic techniques provided additional information about functional and structural perturbations of the enzyme. Isothermal titration calorimetry (ITC) revealed a mixed interaction and a binding model with a large number of molecules of protein per molecule of polyacrylic acid. One the one hand circular dichroism (CD) spectroscopy showed that alpha-amylase loses its secondary structure in the presence of increasing concentrations of polyacrylic acid, while it is stabilized by the polyelectrolyte at low pH. On the other hand, fluorescence spectra revealed that the three-dimensional enzyme structure was not affected in the microenvironment of tryptophan residues. Differential scanning calorimetry (DSC) thermograms showed that only one domain of alpha-amylase is affected in its conformational stability by the polymer. The unfolding process proved to be partially reversible. Finally, the enzyme retained more than 90% of its catalytic activity even in excess of the polymer.Graphical abstractGraphical abstract for this article
       
  • Zn2+-assisted photothermal therapy for rapid bacteria-killing using
           biodegradable humic acid encapsulated MOFs
    • Abstract: Publication date: Available online 8 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ziwei Liu, Lei Tan, Xiangmei Liu, Yanqin Liang, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Shengli Zhu, Zhaoyang Li, Shuilin WuBacterial infection is seriously threatening human health all over the world, especially with the emergence of increasing drug-fast bacteria. It is urgent to develop a drug-free strategy to kill bacteria rapidly and efficiently. In this work, humic acid (HuA) encapsulated zeolitic imidazole framework-8 (ZIF-8) (HuA@ZIF-8) nanocomposites are synthesized by the in-situ growth of ZIF-8 on the surface of polyvinylpyrrolidone (PVP)-modified HuA. The synthesized nanocomposites possesses good photothermal effects, i.e., the temperature increased to 59.4 °C under the particle concentration of 1000 µg/mL with 10 min NIR irradiation. In addition, NIR irradiation can also control the release of Zn2+ from the composites. The good photothermal effects originate from HuA that can effectively absorb NIR light. The controlled release of Zn2+ is ascribed to the induced-dissociation of ZIF-8 under NIR light irradiation. The synergistic action of photothermal therapy and release of zinc ions contributes to the excellent antibacterial efficiency of HuA@ZIF-8 within a short time, i.e. 99.59% and 99.37% against Staphylococcus aureus and Escherichia coli with 20 min NIR irradiation, respectively. This work provides a promising strategy to develop a light-responsive platform with good biodegradability and low cost for rapid and effective sterilization.Graphical abstractGraphical abstract for this article
       
  • Glucose-Induced Structural Changes and Anomalous Diffusion of Elastin
    • Abstract: Publication date: Available online 8 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Debajyoti De, Nisha Pawar, Amar Nath GuptaABSTRACTElastin is the principal protein component of elastic fiber, which renders essential elasticity to connective tissues and organs. Here, we adopted a multi-technique approach to study the transport, viscoelastic, and structural properties of elastin exposed to various glucose concentrations (X=[gluc]/[elastin]). Laser light scattering experiments revealed an anomalous behavior (anomaly exponent, β
       
  • Mechanical and transport properties of chitosan-zwitterionic phospholipid
           vesicles
    • Abstract: Publication date: Available online 8 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Honey Priya James, Sameer JadhavChitosan is a polysaccharide that has shown promise in liposomal drug delivery because of certain desirable properties such as muco-adhesivity, biodegradability and low toxicity. In this study, chitosan-bearing 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine giant unilamellar vesicles were prepared using inverse phase precursor method to measure their mechanical and transport properties. We show that while an increase in chitosan: lipid molar ratio in the vesicle bilayer at pH 7 led to a substantial increase in its bending modulus, chitosan-mediated change in bending modulus was diminished at pH 4.5. Water permeability across the vesicle bilayer, as well as phospholipid diffusivity within supported lipid bilayers, were also found to decrease with increasing chitosan : lipid molar ratio. Together, these findings demonstrate that incorporation of chitosan in phospholipid bilayers modulates the mechanical and transport properties of liposomes which may affect their in vivo circulation time and drug release rate.Graphical abstractGraphical abstract for this article
       
  • Neutrophil membranes coated, antibiotic agent loaded nanoparticles
           targeting to the lung inflammation
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Kaiyu Wang, Yiteng Lei, Donglin Xia, Peipei Xu, Tao Zhu, Zhongying Jiang, Yuqiang MaNatural cellular membranes, with the outstanding qualities of biocompatibility and specificity, have gained growing attentions in the system of drug delivery. Nanoparticles coated with cellular membranes are starting to be applied as drug-loaded-vehicles to target tumors. Here, neutrophil membranes were selected to apply in the treatment of inflammation because neutrophils can participate in various inflammatory responses and accumulate at inflammatory sites to eliminate pathogens. Through extracting neutrophil membranes from natural neutrophils without affecting their biological properties, nanoparticles loaded with sparfloxacin (SPX) were coated with these membranes and disguised as neutrophils. Compared with traditional nano-medicines, the neutrophil membrane-coated nanoparticles (NM-NP-SPX) possessed precise targeting ability just like the neutrophils could accumulate at inflammatory sites when inflammation burst. In addition, NM-NP-SPX could prolong the circulation time and had the property of controlled-release. Through in vivo experiments, we found that the concentration of three representative inflammatory cytokines in blood, bacteria and inflammatory cells in lungs of the mice with pneumonia reduced significantly in the initial 24 h after the injection of NM-NP-SPX, which meant that NM-NP-SPX could greatly reduce the risk of death for the patients with inflammation. Moreover, the infected lungs could recover rapidly without any side effects to other organs due to the low cytotoxicity of NM-NP-SPX against normal cells. Therefore, our developed drug delivery system has enormous advantages in treating inflammations. Not only that, this kind of bionic method may have greater value and application prospects in curing the inflammations arisen from cancers.Graphical abstractGraphical abstract for this article
       
  • The hemocompatibility of the modified polysulfone membrane with
           4-(chloromethyl)benzoic acid and sulfonated hydroxypropyl chitosan
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Sheng Yan, Ming-Ming Tu, Yun-Ren QiuPolysulfone (PSf) membrane is widely employed in blood purification fields, but the blood compatibility of PSf membrane is not adequate. To improve the hemocompatibility of PSf membrane, 4-(chloromethyl)benzoic acid (CMBA) and sulfonated hydroxypropyl chitosan (SHPCS) were grafted onto PSf membrane surface. In our strategy, CMBA was firstly grafted on the PSf membrane surface through the Friedel-Crafts alkylation reaction, and the product was named BAPSf membrane. Then, SHPCS was grafted onto the BAPSf membrane surface by esterification, and the product was named SHPCS-BAPSf membrane. The effects of temperature and reaction time on the productivity of BAPSf and the grafting density of carboxyl and the effects of reaction time on the grafting density of SHPCS grafted onto the BAPSf membrane surface were studied. The SHPCS-BAPSf membranes are investigated by ATR-FTIR, XPS, contact angle measurements and evaluated by blood compatibility in vitro. The results reveal that the hydrophilicity of SHPCS-BAPSf membranes were grealy improved and the evaluation of protein adsorption, hemolysis test, platelet adhesion plasma recalcification time(PRT), activated partial thromboplastin time(APTT), prothrombin time(PT) and thrombin time(TT) confirmed that the SHPCS-BAPSf membranes have remarkable blood compatibility.Graphical abstractGraphical abstract for this article
       
  • Preparation of antimicrobial and antioxidant gelatin/curcumin composite
           films for active food packaging application
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Swarup Roy, Jong-Whan RhimThe functional gelatin/curcumin composite film was prepared using an emulsifier, sodium dodecyl sulfate (SDS). The composite films were characterized using field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR). The FE-SEM test results showed that curcumin was evenly distributed in the gelatin polymer matrix to form a flexible composite film with a smooth surface. The addition of 1.5 % of curcumin improved the UV blocking effect by more than 99 % at a loss of 5.7 % of transparency compared to neat gelatin films. The addition of curcumin (up to 1 wt%) significantly improved mechanical and water vapor barrier properties. Also, the gelatin/curcumin composite films exhibited remarkable antimicrobial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes, and showed strong antioxidant activity comparable to ascorbic acid. Antibacterial and antioxidant gelatin/curcumin composite films with improved UV protection, water vapor barrier and mechanical properties have high potential in active food packaging applications.Graphical abstractGraphical abstract for this article
       
  • Controlled formation of porous CuCo2O4 nanorods with enhanced oxidase and
           catalase catalytic activities using bimetal-organic frameworks as
           templates
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Dan Song, Ting Li, Yun-Yun Wei, Zhang-Run XuPorous structures with highly dispersed and active catalytic sites are vital to improve the catalytic activity and stability of artificial enzyme-related catalytic reactions. Herein, a novel nanorod-like bimetal-organic framework serving as porous support and supplier of Co2+ and Cu2+ was used to prepare a beneficial porous metal oxide. By optimizing the calcination temperature, the composition of calcined product can be controlled and the nanorods with isolated and highly active CuCo2O4 nanoparticles were obtained. The porous CuCo2O4 nanorods exhibit a pH-dependent catalytic property, that is, they behave as oxidase in acid conditions and catalase in alkaline conditions. The CuCo2O4 nanorods perform dual-enzyme catalytic activity superior to monometallic oxides. What’s more, compared with the reported Co3O4 nanoparticles, Co3O4/CuO hollow nanocage hybrids and NiCo2O4 mesoporous spheres, the porous CuCo2O4 nanorods show higher affinity to 3,3′,5,5′-tetramethylbenzidine with a lower Km value. The superior dual-enzyme catalytic activities of CuCo2O4 nanorods benefit from the high catalytic activity of binary metal oxides and structural stability. After incubating in a wide range of pHs, temperatures and ionic strengths, the catalytic activity of CuCo2O4 nanorods can be maintained. The oxidase activity of CuCo2O4 nanorods can be inhibited in the presence of ascorbic acid, which can be applied in effective detection of ascorbic acid. This study opens a new path to prepare stable and highly active porous artificial enzymes.Graphical abstractGraphical abstract for this article
       
  • In situ microemulsion-gel obtained from bioadhesive hydroxypropyl
           methylcellulose films for transdermal administration of zidovudine
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Guilherme Rodolfo Souza de Araujo, Leandro de Oliveira Porfírio, Luiz André Santos Silva, Danielle Gomes Santana, Paula Ferreira Barbosa, Cochiran Pereira dos Santos, Narendra Narain, Victor Hugo Vitorino Sarmento, Rogéria de Souza Nunes, Elizabeth Ting, Ana Amélia Moreira LiraThis study aims to develop in situ microemulsion-gel (ME-Gel) obtained from hydroxypropyl methylcellulose (HPMC) films for transdermal administration of Zidovudine (AZT). Firstly, HPMC films containing propylene glycol (PG) and eucalyptus oil (EO) were obtained and characterized. Later, a pseudo-ternary phase diagram composed of water, EO, tween 80 and PG was obtained and one microemulsion (ME) with a similar proportion of the film components was obtained. ME was transformed in ME-Gel by the incorporation of HPMC. Finally, HPMC films were hydrated with Tween 80 solution to yield in situ ME-Gel and its effect on AZT skin permeation was compared with HPMC film hydrated with water (F5hyd). The results showed that the ME and ME-Gel presented a droplet size of 16.79 and 122.13 μm, respectively, polydispersity index (PDI) < 0.39 and pH between 5.10 and 5.40. The incorporation of HPMC resulted in viscosity about 2 times higher than the use of ME. The presence of AZT did not alter the formulation properties. The in situ ME-Gel promoted a two-fold increase in the permeated amount of AZT compared to F5hyd. The results suggest that it was possible to obtain an ME-Gel in situ from HPMC films and that its effect on transdermal permeation of AZT was significant.Graphical abstractGraphical abstract for this article
       
  • Electrophoretic deposition of polymethylmethacrylate and composites for
           biomedical applications
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): A. D'Elia, J. Deering, A. Clifford, B.E.J. Lee, K. Grandfield, I. ZhitomirskyFor the first time, an electrophoretic deposition (EPD) method has been developed for the deposition of polymethylmethacrylate (PMMA) and PMMA-alumina films for biomedical implant applications. The proposed biomimetic approach was based on the use of a bile salt, sodium cholate (NaCh), which served as a multifunctional solubilizing, charging, dispersing and film-forming agent. Investigations revealed PMMA-Ch− and PMMA-alumina interactions, which facilitated the deposition of PMMA and PMMA-alumina films. This approach allows for the use of a non-toxic water-ethanol solvent for PMMA. The proposed deposition strategy can also be used for co-deposition of PMMA with other functional materials. The PMMA and composite films were tested for biomedical implant applications. The PMMA-alumina films showed statistically improved metabolic results compared to both the bare stainless steel substrate and pure PMMA films. Alkaline phosphatase (ALP) activity affirmed the bioactivity and osteoconductive potential of PMMA and composite films. PMMA-alumina films showed greater ALP activity than both the PMMA-coated and uncoated stainless steel.Graphical abstractGraphical abstract for this article
       
  • Catanionic nanocarriers as a potential vehicle for insulin delivery
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Soledad Stagnoli, Lucas Sosa Alderete, M. Alejandra Luna, Elizabeth Agostini, R. Dario Falcone, Ana M. Niebylski, N. Mariano CorreaDiabetes is a disease that affects millions of people in the World, constituting a global problem. Patients are administered insulin subcutaneous injections, resulting in high costs and frequent infections in the injection site. A possible solution to this problem may be the use of nanotechnology.Nanotransporters can act as specific release systems able to overcome the current limitations to drug delivery. Liposomes and vesicles can deliver drugs directly and efficiently to the site of action, decreasing toxicity and adverse effects. In previous studies, we demonstrated the biocompatibility and safety of catanionic benzyl n-hexadecyldimethylammonium 1,4 -bis-2-ethylhexylsulfosuccinate (BHD-AOT) vesicles using both in vitro and in vivo tests. Thus, the aims of this work were to evaluate the ability of the BHD-AOT vesicles to encapsulate insulin; to analyze the structural properties and stability of the system, vesicle-Insulin (VIn), at different pH conditions; and to study the ability of VIn to decrease the glycemia in miceby different administration routes. Our results showed that 2 and 5 mg mL−1 of vesicles were able to encapsulate about 55 % and 73 % of insulin, respectively. The system VIn showed a significant increase in size from 120 to 350 nm, changes in the surface zeta potential value, and high stability to different pH conditions. A significant decrease of the glycemia after VIn administration was demonstrated in in vivo assays, including the oral route. Our results reveal that BHD-AOT vesicles may be an appropriate system to encapsulate and protect insulin, and may be a potential system to be administrated in different ways as an alternative strategy to conventional therapy.Graphical abstractGraphical abstract for this article
       
  • Polyphotosensitizer nanogels for GSH-responsive histone deacetylase
           inhibitors delivery and enhanced cancer photodynamic therapy
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Nian Liu, Hongsen Liu, Haoran Chen, Gang Wang, Haolin Teng, Yulei ChangPhotodynamic therapy (PDT) is one of the non-invasive and selective treatment methodologies for cancer. However, many highly efficient photosensitizers (PSs) are usually low physiological solubility, limited bioavailability and tending aggregation, impeding the effectiveness of PDT, as well as cancer resistance of PDT further reduce its therapeutic effect. Though some smart delivery systems have been developed, the problem of photosensitizer leakage/release has not been completely solved. Herein, we developed a smart therapeutic nanoplatform based on polyphotosensitizer nanogel as novel nanophotosensitizers and drug carriers. Moreover, by loading of histone deacetylase inhibitors (SAHA), it allows for enhanced synergistic therapy strategy of prostate cancer via inhibiting HIF-1α and VEGF pathways of cancer cells involved in PDT resistance. Our study presents the well-designed nanoplatform of nanogel-Ce6, which could serve as a photodynamic agent without Ce6 molecules release in the responsive environment, offering the potential to encapsulate diverse functional components for smart drug release and imaging-guided combination therapy in vitro and in vivo.Graphical abstractGraphical abstract for this article
       
  • pH-dependent swelling and antibiotic release from citric acid crosslinked
           poly(vinyl alcohol) (PVA)/nano silver hydrogels
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Mohammad Sabzi, Mohammad Javad Afshari, Masoud Babaahmadi, Nima ShafaghIn this work, a pH-sensitive and antibacterial drug delivery system based on poly(vinyl alcohol) (PVA)/citric acid (CA)/Ag nanoparticles (NPs) was designed using a completely green, facile and one-step route. Interestingly, the crosslinking of PVA with CA, and in-situ formation of Ag NPs within the polymeric matrix were simultaneously and simply carried out using an annealing process without need for any toxic chemicals. The developed hydrogels were characterized by FTIR, UV–vis spectra, SEM and TEM techniques. It was found that CA not only acted as a crosslinker of PVA via esterification reaction, but also it endowed pH-responsiveness and antibacterial activity to the PVA matrix due to presence of free carboxylic acid groups on CA. Hydrogels demonstrated a pH-dependent swelling as well as drug release behavior, as the swelling ratio and the drug release at pH 7.4 were found higher than pH 1.2. Furthermore, the release of ciprofloxacin was more sustained when Ag NPs were incorporated into hydrogels. In addition, the incorporation of CA, Ag NPs and ciprofloxacin into the PVA matrix provided an effective antibacterial activity against E. coli and S. aureus microorganisms. The developed hydrogels can be considered as a promising material in the prolonged antibiotic therapy such as intestinal infection treatment.Graphical abstractGraphical abstract for this article
       
  • Systematic approach to characterize the dynamics of protein adsorption on
           the surface of biomaterials using proteomics
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Jinku KimProtein adsorption on biomaterial surfaces has been investigated in the development of protein-repellent implantable devices. While the study of the adsorption of a single protein have produced valuable insights of the role of specific proteins in the biological responses to biomaterials, a systematic high throughput screening method is needed to gain more comprehensive understanding of such a complex process, mainly because biomaterials are exposed to protein mixtures when implanted in the human body. To further advance our knowledge of the dynamics of protein adsorption/desorption at interfaces between proteins and solid surfaces, proteomic technologies have been explored to determine relationships between adsorbed proteins on the surfaces and subsequent biological responses. In this review, we will briefly describe the protein adsorption process and proteomics technologies and focus on subsequent biological responses to biomaterials such as blood/biomaterial interactions, biocompatibility, and cell behavior, to obtain more comprehensive understanding of the process for the development of improved biomaterials. We also highlight a number of challenges of contemporary proteomics technologies and future perspectives to advance our knowledge of protein adsorption/desorption dynamics on the surfaces of biomaterials.Graphical abstractGraphical abstract for this article
       
  • Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for
           enhancement of the therapeutic efficacy in HeLa cancer cells
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Maryam Ghaffari, Gholamreza Dehghan, Behzad Baradaran, Amir Zarebkohan, Behzad Mansoori, Jafar Soleymani, Jafar Ezzati Nazhad Dolatabadi, Michael R. HamblinCo-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.Graphical abstractGraphical abstract for this article
       
  • Novel carrier-free nanoparticles composed of
           7-ethyl-10-hydroxycamptothecin and chlorin e6: Self-assembly mechanism
           investigation and in vitro/in vivo evaluation
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Yanna Zhao, Yuping Zhao, Qisan Ma, Huaizhen Zhang, Yinglin Liu, Jingyi Hong, Zhuang Ding, Min Liu, Jun HanThe combination therapy strategy based on both chemotherapy and photodynamic therapy (PDT) exhibits great potential for advanced cancer treatment. Multimodal nanodrug delivery systems based on both chemotherapeutic drug and photodynamic agent have been proven to possess excellent synergistic efficacy. In this study, 7-ethyl-10-hydroxycamptothecin (SN38) and chlorin e6 (Ce6) were co-assembled into novel carrier-free nanoparticles (SN38/Ce6 NPs) via simple antisolvent precipitation method. As expected, SN38/Ce6 NPs exhibited uniform morphology with a particle size of around 150 nm and a zeta potential of about −30 mV, good stability in aqueous solution/at lyophilized state and high cellular uptake efficiency against murine mammary carcinoma (4T1) cell lines. Besides, enhanced singlet oxygen generation capacity of the nanoparticles was both observed in test-tube and in 4T1 cell lines in contrast with Ce6 injection. Moreover, a ∼85 % inhibition rate of SN38/Ce6 NPs with laser was detected, which was significantly higher (P 
       
  • Toxicological evaluation of naringin-loaded nanocapsules in vitro
           and in vivo
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Renata Gancine Budel, Denise Ajala da Silva, Michele Pereira Moreira, Ana Júlia Figueiró Dalcin, Aline Franzen da Silva, Luiza Reali Nazario, Julia Huppes Majolo, Leonardo Quintana Soares Lopes, Roberto Christ Vianna Santos, Felix Alexandre Antunes Soares, Rosane Souza da Silva, Patrícia Gomes, Carina Rodrigues BoeckNaringin is a flavonoid widely known for its pharmacological properties, such as: anti-inflammatory and antioxidant ones, being an ally to avoid oxidative damage. Although naringin is an active easily found in citrus fruits, it has low bioavailability, biodistribution and also undergoes biotransformation in naringenin, limiting the described effects. The use of nanocapsules as drug carriers may increase solubility, improve biodistribution, impede the biotransformation thereof, and thus could improve the performance of naringin for use in treating neurological diseases. Therefore, the objective of this work is to produce a nanocapsule containing naringin, validate an analytical method by RP-HPLC to determination of the drug in nanoparticle and evaluate the toxicity. To that end, the blank nanocapsules (NB, without the drug) or naringin-loaded nanocapsules (NN) at the concentration of 2 mg/mL were prepared by interfacial deposition of the preformed polymer and the quantification of naringin by HPLC. Toxicity of the formulations was evaluated in vitro in rat hippocampal slices and in vivo models with C. elegans and Danio rerio (zebrafish). The analytical parameters evaluated (linearity, limit of detection and quantification, specificity, precision, accuracy and robustness) indicated adequate method to assay of naringin in nanocapsules by HPLC. There was no indication of toxicity by the nanocapsules in the evaluated biological assays.Graphical abstractGraphical abstract for this article
       
  • Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG)
           composite nanofibers: A potential scaffold for tissue engineering
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Hui-Li Tan, Dan Kai, Pooria Pasbakhsh, Sin-Yeang Teow, Yau-Yan Lim, Janarthanan PushpamalarElectrospinning is a common method to prepare nanofiber scaffolds for tissue engineering. One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied. However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion. In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG). Different ratios of CAB to PEG were tested and only the ratio of 2:1 resulted in smooth and bead-free nanofibers. The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers. The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis. As the hydrophilicity increases, the swelling ability of the composite nanofiber increases by 2-fold with more rapid biodegradation. The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF). The cell viability assay results revealed that the nanofibers are non-toxic. In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers. Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.Graphical abstractGraphical abstract for this article
       
  • A colorimetric strategy for ascorbic acid sensing based on the
           peroxidase-like activity of core-shell Fe3O4/CoFe-LDH hybrid
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Wenning Yang, Jia Li, Mingyue Wang, Xiaofan Sun, Yong Liu, Jie Yang, Dickon H.L. NgA novel core-shell Fe3O4/CoFe-LDH (layered double hydroxides) hybrid as a peroxidase mimic for the colorimetric detection of ascorbic acid was first fabricated via a facile two-step route. The resulting Fe3O4/CoFe-LDH hybrid exhibited much higher peroxidase-like catalytic activity for the oxidation of 3,3′,5,5′- tetramethylbenzidine (TMB) with H2O2 than the pristine Fe3O4 and CoFe-LDH nanosheets owing to the unique hierarchical architecture containing more exposed active sites and the synergistic effect between Fe3O4 and CoFe-LDH. A sensitively and selectively visual sensor for the determination of ascorbic acid (AA) was successfully constructed based on the reduction effect of AA with enediol group on the formed oxidation of TMB, which exhibited a sensitive response to AA in the range of 0.5 ∼ 10 μM with the detection limit of 0.2 μM. Additionally, the Fe3O4/CoFe-LDH magnetic hybrid could be easily recycled by applying a magnetic field. This work provided a feasible means for the fabrication of magnetic nanomaterials with encouraging prospect in biosensing, environmental monitoring and medical diagnostics.Graphical abstractGraphical abstract for this article
       
  • How is the interaction of a chloride channel blocker with phospholipids
           influenced by divalent metal ions' Effect of unsaturation on the lipid
           side chain
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Riya Sett, Bijan K. Paul, Swagata Sen, Nikhil GuchhaitThe present study reveals the effect of various divalent ions (Ca2+, Mg2+and Zn2+) on the binding interaction of a prospective chloride channel blocker, 9-methylanthroate (9MA), with liposome membranes, namely, dimyristoylphosphatidylcholine (DMPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The liposome membranes DMPC and POPC differ in the unsaturation of the side-chain. The drug (9MA) is found to experience a greater degree of partitioning into the POPC lipid bilayer (containing unsaturated side-chain) in comparison to DMPC (containing saturated side-chain). The stronger 9MA-POPC binding interaction is found to be only nominally perturbed by the presence of metal salts. On the contrary, the 9MA-DMPC binding interaction is found to be significantly perturbed by the presence of metal salts and is manifested on the environment-responsive spectroscopic properties of the drug. The steady-state and picosecond-resolved fluorescence spectroscopic results reveal the effect of metal ions on DMPC bilayer to follow the trend Ca2+ < Mg2+ < Zn2+. This is also quantified by evaluating the partition coefficient of the drug into DMPC lipid in the presence of various divalent ions which is found to follow the same sequence. The degree of penetration of these cations has been rationalized on the basis of adsorption of cations on DMPC headgroup region resulting in dehydration of the headgroup along with shrinking of it.Graphical abstractGraphical abstract for this article
       
  • Biological effects on tooth root surface topographies induced by various
           mechanical treatments
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Xiaohui Qiu, Shuo Xu, Yuanping Hao, Brandon Peterson, Baowei Li, Kai Yang, Xiaofang Lv, Qihui Zhou, Qiuxia JiThe cleaning and physicochemical properties on tooth root biointerfaces are pivotal for periodontal healing. Herein, this work investigated the impact of multi-treatment on the physicochemical features of tooth root surfaces and the responsive behavior of human gingival fibroblasts (hGFs). It was found that the combination of various mechanical treatments significantly affects the topographical pattern and size as well as wettability on tooth root surfaces. Furthermore, biological experiments revealed that hGF behaviors (i.e., cell adhesion, shape, spreading, arrangement, and viability) were regulated by the topography and wettability of tooth root surfaces. Also, there was no significant difference in the protein expression of NLRP3 inflammasome and IL-1β in hGFs among tooth root surfaces under various treatments. This study provides new insights to efficiently remove the dental calculus and to understand the interaction between the tooth root interface and cell, which could guide the clinical operation and thereby is more conducive to periodontal recovery.Graphical abstractGraphical abstract for this article
       
  • Influences of sodium tantalite submicro-particles in polyetheretherketone
           based composites on behaviors of rBMSCs/HGE-1 cells for dental application
           
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Lishu Ren, Songchao Tang, Xuening Shen, Chao Gao, Yun Kyung Jung, Dongliang Wang, Axiang He, Lili Yang, Jung-Woog Shin, Jie WeiDental implanted materials require excellent mechanical properties, biocompatibility as well as integration with bone tissue and gingival tissue to achieve early loading and long-term stability. In this study, cubic shape sodium tantalite (ST) submicro-particles with the size of around 180 nm were synthesized by a hydrothermal method, and ST/polyetheretherketone (PEEK) composites (TPC) with ST content of 20 w% (TPC20) and 40 w% (TPC40) were prepared by melting blend. The results showed that the compressive strength, thermal properties, surface roughness, hydrophilicity and surface energy as well as adsorption of proteins on TPC40 were also significantly enhanced compared with TPC20 and PEEK. Moreover, the responses (adhesion and proliferation as well as differentiation) of rat bone marrow mesenchymal stem cells (rBMSCs), and responses (adhesion, and proliferation) of human gingival epithelial (HGE-1) cells to TPC40 were significantly promoted compared with TPC20 and PEEK. The results demonstrated that ST content in TPC had remarkable effects on the surface properties, which played key roles in stimulating the responses of both rBMSCs and HGE-1 cells. TPC40 with increased surface properties and excellent cytocompatibility might have great potential as an implanted material for dental application.Graphical abstractGraphical abstract for this article
       
  • Comparison of Tanaka lipid mixture with natural surfactant Alveofact to
           study nanoparticle interactions on Langmuir film balance
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Julia Janina Schüer, Christian Wölk, Udo Bakowsky, Shashank Reddy PinnapireddyUpon inhalation, nanoparticles enter the lungs where the pulmonary surfactant forms the first point of contact and plays a pivotal role for the subsequent absorption into the body. This can lead to interactions that alter the biophysical function of the surfactant monolayer. Therefore, a reliable prediction of the interaction is desired. In this study, we compared the behaviour of an artificial surfactant model with that of a natural surfactant upon exposure to chitosan nanoparticles. To simulate the physiology of the lungs, the surfactant monolayers were placed at an air/aqueous interface of a Langmuir film balance. Based on the data obtained from the experiments, the chitosan nanoparticles first integrated into the monolayer of the natural surfactant and then interact strongly with its compounds thereby moving out of the monolayer. The topographic changes in the monolayer were determined by atomic force microscopy analysis. Using this technique, the nanoparticle localisation on the monolayer could be studied. No visible interaction was observed with the artificial surfactant from surface pressure-time isotherms and atomic force microscopy analysis. Incomplete miscibility lead to instability of the artificial surfactant which left behind a DPPC rich monolayer after nanoparticle interaction. It was not stable enough to see a possible interaction (i.e. change in surface pressure) with the nanoparticles directly. These results should help understand the interactions of lipids among themselves and with the nanoparticles. Furthermore, it should help generate an efficient artificial surfactant model and to understand the underlying mechanisms of the nanoparticle interaction with the monolayer.Graphical abstractGraphical abstract for this article
       
  • Spatiotemporal variations of contact stress between liquid-crystal films
           and fibroblasts Guide cell fate and skin regeneration
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Jing Chen, Ye Yang, Jingjing Wu, Xue Rui, Wei Wang, Rongrong Ren, Qingqing Zhang, Qingqing Chen, Dengke YinThe inductions of both the mechanical microenvironment on cell behaviour and the polymeric scaffold on tissue regeneration have been well-proved. This study is aimed to investigate the possibility of guiding cell fate and tissue regeneration by the spatiotemporal controlling of contact stress between matrix materials and cells and to elucidate the mechanisms underlying. A series liquid crystal polymers of cholesteryl-oligo(lactic acid) (CLA) and an amorphous polymer of poly(lactic acid) were used as the growth substrates for fibroblast and skin tissue regeneration. The cellular and animal experiments show that, in the initial stage of wound healing, the liquid crystal texture of CLA films can provide an induced stress for the formation of focal adhesions and the activation of integrin β1/AKT signal pathway, resulting in advanced phenotypic transformation of fibroblasts to myofibroblasts, promoted collagen secretion and fast wound filling. But the gradually weakening cellular contact stress, induced by the decreasing of liquid crystal domains of matrix polymer during degradation, triggers the apoptosis of fibroblasts and myofibroblasts, resulting in non-excessive collagen accumulation. Finally, the CLA groups exhibit no obvious scar formation, more regular cell arrangement and significantly lower type I collagen proportion in regenerated tissue than other groups. This study may inspire a new, effective and safe strategy for tissue regeneration.Graphical abstractGraphical abstract for this article
       
  • Carbohydrate coated fluorescent mesoporous silica particles for bacterial
           imaging
    • Abstract: Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Haritha Kirla, Leonie Hughes, David J. HenryThis work investigated the synthesis of carbohydrate functionalized methylene blue doped amine grafted mesoporous silica nanoparticles (MB AMSN) and their application in bioimaging. A single-pot synthesis methodology was developed via a modified co-condensation sol-gel technique for simultaneous incorporation of the dye molecule in the nanoparticles, with amine grafting for subsequent functionalization. The obtained nanoparticles (∼ 450 nm) are mesoporous and have a high surface area (538 m2/g), pore-volume (0.3 cm3/g), showed excellent UV-vis absorbance, and dye encapsulation efficiency (> 75 %). These fluorescent nanoparticles were further functionalized with carbohydrate molecules before application as contrast agents in bacterial cells. In the present study, gram-positive (E. coli) and gram-negative (B. subtilis) bacteria were used as model organisms. Confocal laser microscopy results showed that the nanoparticles are highly fluorescent, and SEM of glucose conjugated MB doped nanoparticles indicated close interaction with E. coli with no toxicity observed towards either bacterial cells. The results demonstrate that by suitable surface functionalization, the methylene blue doped silica nanoparticles can be used as bioimaging agents.Graphical abstractGraphical abstract for this article
       
  • Feasibility of cationic carbosilane dendrimers for sustainable protein
           sample preparation
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Estefanía González-García, Javier Sánchez-Nieves, Francisco Javier de la Mata, María Luisa Marina, María Concepción GarcíaProtein sample preparation is the bottleneck in the analysis of proteins. The aim of this work is to evaluate the feasibility of carbosilane dendrimers functionalized with cationic groups to make easier this step. Anionic carbosilane dendrimers (sulphonate- and carboxylate-terminated) have already demonstrated their interaction with proteins and their potential in protein sample preparation. In this work, interactions between positively charged carbosilane dendrimers and different model proteins were studied when working under different pH conditions, dendrimer concentrations, and dendrimer generations. Amino- and trimethylammonium-terminated carbosilane dendrimers presented, in some cases, weak interactions with proteins. Unlike them, carbosilane dendrimers with terminal dimethylamino groups could interact, in many cases, with proteins and these interactions were affected by the pH, the dendrimer concentration, and the dendrimer generation. Moreover, dendrimer precipitation was observed at all pHs, although just second and fourth generation (2 G and 4 G) dendrimers resulted in the formation of complexes with proteins. Under experimental conditions promoting dendrimer-protein interactions, 2 G dimethylamino-terminated dendrimers were proposed as an alternative to other methods used in analytical chemistry or analysis in which an organic solvent or a resin are required to enrich/purify proteins in a complex sample.Graphical abstractGraphical abstract for this article
       
  • The role of the intestinal-protein corona on the mucodiffusion behaviour
           of new nanoemulsions stabilised by ascorbyl derivatives
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): M. Plaza-Oliver, M.J. Santander-Ortega, L. Castro-Vázquez, V. Rodríguez-Robledo, J. González-Fuentes, P. Marcos, M.V. Lozano, M.M. Arroyo-JiménezNanoemulsions are vesicular systems with great potential for the delivery of drugs, which significantly depends on the appropriate selection of the components that constitute them. In this sense, the use of materials with adequate toxicity profiles for the oral route provides additional advantages in terms of safety concerns avoidance. This work describes the formulation of novel two-component nanoemulsions constituted by α-tocopherol and ascorbyl-palmitate derivatives. Among them, ascorbyl-dipalmitate allowed the formation of nanoemulsions with size values around 170 nm and negative charge; additionally, they showed strong antioxidant capacity. These nanoemulsions are proposed to the oral route, so their behaviour in intestinal conditions was evaluated by incubating the nanoemulsion in simulated intestinal fluid. This process led to the formation of an intestinal-protein corona (I-PC) at the colloidal surface that determined the interaction with the mucus barrier. The I-PC displaced the immobile-hindered particles towards a subdiffusive-diffusive population. These studies report for the first time the effect of the I-PC on the mucodiffusion behaviour of vesicular systems, a finding that may help to comprehend the performance of nanocarriers under intestinal conditions.Graphical abstractGraphical abstract for this article
       
  • Antibacterial and anticancer activities of asymmetric lollipop-like
           mesoporous silica nanoparticles loaded with curcumin and gentamicin
           sulfate
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Yajun Cheng, Yudi Zhang, Weijun Deng, Jing HuAsymmetric mesoporous silica nanoparticles with anisotropic geometry and dual-compartments are highly desired for loading and release of dual-drugs in separated storage spaces. In this study, an asymmetric lollipop-like mesoporous silica nanoparticle Fe3O4@SiO2&EPMO (EPMO = ethane bridged periodic mesoporous organosilica) was successfully developed via an anisotropic epitaxial growth strategy. The asymmetric nanoparticles show a uniform lollipop shape with a head of spherical Fe3O4@SiO2 core-shell that is 200 nm in diameter and a tail of EPMO nanorods with a length of ∼90 nm, and a specific surface area of ∼650.3 m2 g−1. Most importantly, the asymmetric nanoparticles possess the unique dual independent (hydrophilic/hydrophobic) spaces with good loading capacities and are significantly more efficient for cancer cell killing than pure drug based on in vitro studies. Additionally, the dual-drug-loaded nanoparticles exhibited excellent antibacterial activity.Graphical abstractGraphical abstract for this article
       
  • Monitoring the morphological evolution of giant vesicles by azo dye-based
           sum-frequency generation (SFG) microscopy
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Atsuya Momotake, Takaha Mizuguchi, Mafumi Hishida, Yasuhiko Yamamoto, Masato Yasui, Mutsuo NuriyaIn the present work, dye-based sum-frequency generation (SFG) imaging using sodium 4-[4-(dibutylamino)phenylazo]benzenesulfonate (butyl orange, BO) as a new non-fluorescent specific azo dye is employed to monitor the morphological evolution of giant vesicles (GVs). After loading BO to the membrane of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) single-component GVs, the outermost membranes were clearly visualized using SFG microscopy, which provided images of the distinct outer and inner faces of the lipid bilayers. In addition, SFG-active vesicles were detected also inside the GVs, depending on the dye concentrations. The dye-based SFG imaging technique provided experimental evidence that these oligolamellar vesicles containing an SFG-active interior had been formed after BO loading. The formation process of the oligolamellar vesicles with inner SFG-active vesicles was successfully monitored, and their formation mechanism was discussed.Graphical abstractGraphical abstract for this article
       
  • Effect of cosurfactant addition on phase behavior and microstructure of a
           water dilutable microemulsion
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Pooja Golwala, Sachin Rathod, Rahul Patil, Amita Joshi, Debes Ray, Vinod K. Aswal, Pratap Bahadur, Sanjay TiwariThis study reports a detailed characterization of a nonionic microemulsion (μE) composed of n-butylacetate/α-tocopheryl polyethylene glycol succinate (TPGS)/alcohol/water. Two approaches of expanding the monophasic area were explored; (i) addition of Pluronic® 123 (P123) in aqueous phase, and (ii) use of short chain alcohol (CnHn+1OH; n = 2–4) as cosurfactant. Pseudo-ternary phase diagrams were constructed using water titration method. Characterizations were performed using dynamic light scattering (DLS), differential scanning calorimetry (DSC), small angle neutron scattering (SANS) and electron microscopic techniques. DSC and SANS results showed gradual structural transformation from water-in-oil to oil-in-water system. The optimized formulation (oil/Smix/water – 19/40/41) showed average hydrodynamic diameter of 22 nm, consistent with electron microscopic observations.Ethanol (EtOH), with its high fluidity and smaller headgroup area, offered maximum expansion in the phase boundary. Surfactant unimers, derived from EtOH-driven de-micellization, reinforced the interface and solubilized the incoming oil molecules. Oil incorporation was accompanied with improved loading of carbamazepine, a hydrophobic drug. Except marginal swelling, no significant microstructural changes were noticed during water dilution (≈90%) and salt addition (0.9% NaCl) in the optimized μE formulation. A linear increase in oil incorporation was noticed upon adding propylene glycol as a cosolvent.Graphical abstractGraphical abstract for this article
       
  • Topical application of silk fibroin-based hydrogel in preventing
           hypertrophic scars
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Zheng Li, Jiangbo Song, Jianfei Zhang, Kaige Hao, Lian Liu, Baiqing Wu, Xinyue Zheng, Bo Xiao, Xiaoling Tong, Fangyin DaiCurrent medications for the treatment of hypertrophic scars suffer from bottlenecks of limited therapeutic efficacy and a slow recovery rate. Silk fibroin (SF) has gained attention for its ability to promote wound healing in burns and cutaneous wounds, but its therapeutic effects against hypertrophic scar have not been thoroughly investigated. We prepared SF-based hydrogels (SFHs) with various SF concentrations (1.5 %, 3 %, and 6 %) and characterized their physicochemical properties. Cell experiments showed that these SFHs had favorable biocompatibility in vitro. Further animal experiments in rabbits revealed that the SFH (3 %)-treated group achieved scars on their ears that were thinner and significantly lighter in color compared with the negative control group. Moreover, treatment with SFHs reduced the density and led to the orderly arrangement of collagen fibers. It was found that the therapeutic effects of SFHs were attributed to the reduced expression levels of α-smooth muscle actin. These results are the first to demonstrate that SFH can be exploited as an effective therapeutic agent for the treatment of hypertrophic scars.Graphical abstractGraphical abstract for this article
       
  • Antibacterial and antioxidant activity of exopolysaccharide mediated
           silver nanoparticle synthesized by Lactobacillus brevis isolated from
           Chinese koumiss
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Haichao Zhang, Muhammad Ashraf, Huiyan Fang, Xierong Zeng, Yiguang Wu, Mohsin Khurshid, Liqing Zhao, Zhendan HeRecently, silver nanoparticles gain significant attention due to their applications in various fields. The aim of present study was to develop the eco-friendly, cost effective, and simple method to biosynthesized the silver nanoparticle using sliver nitrate as precursor. In this study, we investigated the physical characterization and biotechnological applications of biosynthesized silver nanoparticle using exopolysaccharide of probiotic Lactobacillus brevis MSR104 isolated from Chinese koumiss. Biosynthesized silver nanoparticles were characterized using the fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction analysis, and elemental analyzer. The achieved results indicate that silver nanoparticles varied in sized with an average size of 45 nm. The X-ray diffraction analysis results showed that the silver nanoparticles have a crystalline nature. The results of antimicrobial assay indicated that the silver nanoparticles exhibited outstanding antimicrobial activity in dose dependent manner against both Gram’s negative as well as Gram’s positive. The antioxidant results indicate that the silver nanoparticles showed excellent scavenging rate against DPPH free radicals (81.4 ± 1.2%) and nitric oxide free radicals (75.06 ± 0.4%). Furthermore, the results of MTT assay revealed that the AgNPs significantly reduced the percentage of live HT-29 cells at higher concentration. This study concluded that the newly synthesized silver nanoparticles have antibacterial, antioxidant, and anticancer applications in agricultural and food industries.Graphical abstractGraphical abstract for this article
       
  • Beyond electrostatic interactions: Ligand shell modulated uptake of
           bis-conjugated iron oxide nanoparticles by cells
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Roberta M. Cardoso, Daiana K. Deda, Sergio H. Toma, Mauricio S. Baptista, Koiti ArakiThe effect of the ligand shell on the cellular uptake efficiency was evaluated by a systematic study using fully dispersed 6 nm diameter superparamagnetic iron oxide nanoparticles (SPIONs), mono and bis-conjugated with glycerol phosphate (glyc), dopamine (dopa), 4,5-dihydroxy-1,3-benzenedisulfonic acid (tiron) and phosphorylethanolamine (pea). Negatively charged SPION-glyc was more efficiently incorporated than positively charged SPION-pea and SPION-dopa clearly evidencing that there are strong enough short-range interactions in addition to the long-range electrostatic interactions, as measured by the zeta potential, to reverse our expectation on cellular uptake. Those effects were pursued by correlating the nanoparticles incorporation efficiency as a function of the respective zeta potentials and the molar fractions of glyc and pea ligands co-conjugated on the SPION surface. The possibility of associating different ligands to modulate the physicochemical properties and biological events was demonstrated, showing promising perspectives for the development of multifunctional nanosystems for biomedical applications.Graphical abstractGraphical abstract for this article
       
  • Inhibition of biofilm formation by rough shark skin-patterned surfaces
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Hsiu-Wen Chien, Xiang-Yu Chen, Wen-Pei Tsai, Mengshan LeeIn this study, we investigate the microscale structure of shark skin denticles at abdomen (A) and fin (F) locations, analyze the roughness and wetting properties related to their microstructures, and evaluate the effect of the surface properties on early bacterial attachment and biofilm formation. Microstructural analysis by scanning electron microscopy and confocal laser scanning microscopy confirmed the length (A: 165–180 μm vs. F: 145–165 μm), width (A: 86–100 μm vs. F: 64–70 μm), height (A: 10.5–13.5 μm vs. F: 6.2–8.8 μm), and density (A: 110–130 denticles/mm2 vs. F: 80–130 denticles/mm2) of the denticles. The results showed that the roughness and hydrophobicity properties were affected with slight differences in the microscale architecture. The denticles with a larger width, higher ridge, and denser overlap provided a rougher and more hydrophobic surface. The microscale structure not only affected surface properties but also the biological attachment process. The microscale topography of shark skin slightly promoted bacterial attachment at an early stage, but prevented bacteria from developing biofilms. This systematic investigation provides insights into the effects of the surface topography of shark skin on its anti-fouling mechanism, which will enable the future development of various products related to human activity, such as healthcare products, underwater devices and applications, and water treatment applications.Graphical abstractGraphical abstract for this article
       
  • Effect of drug molecular weight on niosomes size and encapsulation
           efficiency
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Pablo García-Manrique, Noelia D. Machado, Mariana A. Fernández, María Carmen Blanco-López, María Matos, Gemma GutiérrezEncapsulation into nanocarriers, such as niosomes, is a promising way to protect them from degradation, and allow controll and target delivery of bioactive compounds. For biotechnological applications, a tight control of particle size with acceptable encapsulation efficiencies (EE) is a technological challenge, especially for hydrophilic compounds due to its capability to diffuse across biological barriers. Niosomes formulated with mixture of surfactants represent promising nanocarriers due to the advantages of non-ionic surfactants, such as low cost, versatility and enhanced physico-chemical properties. In this work, the effect of both, composition of the hydrating solution and molecular weight of the loaded compound, on the particle size and EE of niosomes prepared by using the thin film hydration method was studied. Particularly, mili-Q water, glycerol solution and PEG-400 solution were tested for niosomes formulated with Span®80-Tween®80 with/without dodecanol as membrane stabilizer. It was found that particle size highly depends on hydration media composition and an interaction with compound MW could exist. Larger vesicles results in an increase in EE, which could be purely related with physical aspects such as vesicle loading volume capacity. The effect of hydration solution composition could be related with their ability to change the bilayer packing and physical properties, as observed by differential scanning calorimetry. Finally, it was possible to compare the suitability of dialysis and gel filtration as purification methods, demonstrating that gel filtration is not an adequate purification method when viscous solutions are used, since they could affect the particle vesicles retention and hence EE measurements would be misrepresentative.Graphical abstractGraphical abstract for this article
       
  • Encapsulating curcumin in ethylene diamine-β-cyclodextrin nanoparticle
           improves topical cornea delivery
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Chi-Hsien Liu, Guan-Wei Lee, Wei-Chi Wu, Chun-Chao WangCurcumin is a powerful scavenger of reactive oxygen species and could prevent the corneal cells from oxidative damage. However, the clinical efficacy of curcumin is limited by its low aqueous solubility and stability, leading to poor bioavailability. β-cyclodextrin, with a hydrophilic surface and a hydrophobic cavity and self-assembling properties, can form inclusion complexes with lipophilic drugs such as curcumin for ocular delivery. We synthesized ethylene diamine (EDA)-modified β-cyclodextrin and prepared the curcumin complexation using the solvent evaporation method. The EDA-β-cyclodextrin provided a better thermodynamic stability and higher complex yield for curcumin complexes, compared to β-cyclodextrin, which were demonstrated on the analysis of their van’t Hoff plots and phase solubility diagrams. We characterized EDA-β-cyclodextrin curcumin nanoparticles and determined that the EDA modified β-cyclodextrin is a more suitable carrier than parental β-cyclodextrin, using FT-IR, XRD, TEM, and analyses of solubility and storage stability. In addition, the curcumin-EDA-β-cyclodextrin nanoparticles had better in vitro corneal penetration and 3 -h cumulative flux in a porcine cornea experiment, and displayed an improved biocompatibility, confirmed by the histological examination of porcine corneas and cell viability of bovine corneal epithelial cells. These results together revealed a role of EDA modification in the β-cyclodextrin carrier, including the improvement of curcumin complex formation, thermodynamic properties, cytotoxicity, and the in vitro corneal penetration. The EDA-β-cyclodextrin inclusion can provide curcumin a higher degree of aqueous solubility and corneal permeability.Graphical abstractGraphical abstract for this article
       
  • Porous gold layer coated silver nanoplates with efficient antimicrobial
           activity
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Jianfeng Zhu, Shiyi Liu, Taixing Zhang, Yuchen Zhang, Xudong Zhang, Xueqi Liu, Zuoxiu Tie, Yue Dou, Zhenda Lu, Yong HuAlthough silver nanoparticles are considered as promising antibacterial agents because of their antibacterial activity, the acute cytotoxicity of Ag+ released from Ag nanoparticles restricts their potential practical applications. Herein, porous Ag@Au nanoplates, which could balance the Ag+ release and the toxicity of Ag naoparticles, were fabricated by stepwise seed-mediated growth and oxidation. Laser irradiation further boosted their antimicrobial activity, and significantly accelerated the curing rate of wound. Comparing with Ag nanoplates, the irradiated porous Ag@Au nanoplates showed the similar antibiotic ability against S. aureus strains and lower cytotoxicity in vitro. When the porous Ag@Au nanoplates were applied to treat S. aureus-infected wound, they had the best curing effect. Thus, these porous Ag@Au nanoplates could act as promising antibacterial agents for wound healing applications.Graphical abstractGraphical abstract for this article
       
  • Rationally designed magnetic poly(catechol-hexanediamine) particles for
           bacteria removal and on-demand biofilm eradication
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Zhenqiang Shi, Yi Zhang, Rong Dai, Shengqiu Chen, Man Zhang, Lunqiang Jin, Jingxia Wang, Weifeng Zhao, Changsheng ZhaoIn this study, we proposed a green, facile and low-cost approach for the fabrication of multifunctional particles with robust bacteria removal capability and on-demand biofilm eradication activity. Based on mussel-inspired coating of catechol and hexanediamine on Fe3O4 in aqueous solution, magnetic poly(catechol-hexanediamine) particles (Fe3O4@HDA) were prepared successfully in 1 h, at room temperature. Microbiological experiments demonstrated the Fe3O4@HDA particles could capture bacteria in water efficiently. Meanwhile, with an integration of magnetic response property and near-infrared-triggered photothermal bactericidal activity, the Fe3O4@HDA particles showed a high potential for biofilm targeting and in-situ eradication. We believe that the rationally designed magnetic poly(catechol-hexanediamine) particles could extend the applications of smart antimicrobial agents to industrial fields such as water disinfection and biofouling clean-up.Graphical abstractGraphical abstract for this article
       
  • Improving the clearance of protein-bound uremic toxins using cationic
           liposomes as an adsorbent in dialysate
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Yue Shen, Yifeng Wang, Yuanyuan Shi, Xiao Bi, Jingyi Xu, Qiuyu Zhu, Feng DingAnionic and protein-bound uremic toxins, represented by indoxyl sulfate (IS), may be associated with cardiovascular outcomes and the progression of chronic kidney disease in cases of injured kidney function and are not easily cleared by traditional dialysis therapy. We fabricated cationic liposomes that were modified with polyethyleneimine (PEI), octadecylamine (Oct), and hexadecyl trimethyl ammonium bromide (CTAB), and evaluated the effects on the clearance of the representative protein-bound uremic toxins (PBUTs). The binding rate was obtained by ultrafiltration and in vitro dialysis was performed in a Rapid Equilibrium Dialysis (RED) device to assay the clearing efficiency of the dialysate supported by three types of cationic liposomes. The cationic liposomes showed a higher binding rate with IS (1.24–1.38 fold higher) and p-cresol (1.07–1.09 fold higher) than in the unmodified plain liposomes. The dialysate supported by cationic liposomes also exhibited better clearing efficiency for IS (PEI-20: 57.65 ± 1.74 %; Oct-5: 62.80 ± 0.69 %; CTAB-10: 66.54 ± 0.91 %; p 
       
  • Bioinspired DNA nanocockleburs for targeted delivery of doxorubicin
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Si Sun, Nihad Cheraga, Han-Ning Jiang, Qian-Ru Xiao, Peng-Cheng Gao, Yang Wang, Ying-Ying Wei, Xiao-Wei Wang, Yong JiangA variety of three-dimensional DNA assemblies have been proposed as drug carriers owing to their good biocompatibility and easy fabrication. In this study, inspired by the structure of cockleburs, a novel aptamer-tethered DNA assembly was developed for effective targeted drug delivery. The Apt-nanocockleburs were fabricated via a facile process of DNA base pairing: four complementary DNA single strands, including one aptamer-ended strand and three sticky-end strands, were applied to pair with each other. The main body of the nanocockleburs can load doxorubicin (Dox) whilst the covered aptamer spines bind to the target MCF-7 cells. The self-assembled Apt-nanocockleburs exhibit higher cell uptake as well as increased cytotoxicity to MCF-7 cells than DNA nanocockleburs without aptamers. This study provided a DNA constructing platform to produce new drug carriers with high selectivity for cancer targeted drug delivery.Graphical abstractGraphical abstract for this article
       
  • Stable ZnO-doped hydroxyapatite nanocoating for anti-infection and
           osteogenic on titanium
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Baikere Maimaiti, Naiyin Zhang, Ling Yan, Jianghong Luo, Chaoming Xie, Yingbo Wang, Chuang Ma, Tingjun YeTitanium and titanium alloys have been widely used in orthopedics and related fields. However, their clinical applications are limited due to the lack of anti-infection, osteoinductivity and angiogenic ability. In the present study, we utilized pulse electrochemical deposition method to prepare polypyrrole (PPy) by the in-situ oxidative polymerization of pyrrole (Py), and through the coordination and doping of ions, the function of PPy as a dual regulator of hydroxyapatite nanoparticles (HA-NPs) and zinc oxide nanoparticles (ZnO-NPs) was achieved. Bioactivity test showed that the composite coating could induce the formation of apatite, and the apatite was in a neat arrangement preferentially grew along the (002) crystal plane, indicating good bioactivity. The release test showed that the dual regulation effect of PPy coordination and doping reduced the release rate of Ca2+ and Zn2+ from the composite coating. Antibacterial tests showed that the composite coating against Escherichia coli and Staphylococcus aureus. Besides, bone marrow-derived mesenchymal stem cells (BMSCs) exhibited good adhesion, proliferation and differentiation on the composite coating, and fluorescence staining experiments demonstrated good osteoinductivity of the composite coating. In this study, a multifunctional composite coating with anti-infection, angiogenic and osteoinductivity was successfully constructed on the titanium surface via pulse electrochemical deposition method.Graphical abstractThe preparation of HA/PPy/ZnO nanocomposite coating on titanium substrate with bioactive, physiological stable, antibacterial, angiogenic and osteoinductive properties.Graphical abstract for this article
       
  • Polydopamine as a stable and functional nanomaterial
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Salima El Yakhlifi, Vincent BallThe mussel inspired chemistry of dopamine leading to versatile coatings on the surface of all kinds of materials in a one pot process was considered as the unique aspect of catecholamine for a long time. Only recently, research has been undertaken to valorize the simultaneous oxidation and colloid formation in dopamine solutions in the presence of an oxidant. This mini review summarizes the synthesis methods allowing to get controlled nanomaterials, either nanoparticles, hollow capsules or nanotubes and even chiral nanomaterials from dopamine solutions. Finally the applications of those nanomaterials will be described.Graphical abstractGraphical abstract for this article
       
  • Application of a mechanically responsive, inflammatory macrophage-targeted
           dual-sensitive hydrogel drug carrier for atherosclerosis
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Shun Yu Yao, Mei Li Shen, Shao Jing Li, Xiao Dong Wu, Meng Meng Zhang, Li Na Ma, Ya Peng LiAtherosclerotic lesions create obvious vascular stenosis due to the presence of plaque, and a large number of inflammatory macrophages are enriched in the thrombus. In this study, we develop a composite hydrogel drug delivery system that is capable of both mechanically-sensitive drug release and of targeting inflammatory macrophages at the thrombus. The hydrogel is a high molecular weight hyaluronic acid (HA) modified with glycidyl methacrylate as a hydrogel precursor; a cross-linkable block copolymer (CBC) is used as the drug coating material and a microscopic cross-linking agent. The difference in drug release rate of the composite hydrogel (HACBC) in simulated blood vessels with 0 % and 75 % occlusion was as high as 49.3 %. Under long-term cycling conditions in stenotic vessels, dynamic shear rheometry revealed that the HACBC still maintained the hydrogel properties. However, the micelles were deformed and recombined to produce smaller sized micelles. An in vitro cell culture demonstrated precise targeting of the HACBC to inflammatory macrophages, and our rabbit experiments with simvastatin-coated HACBC confirmed the effective release of simvastatin in the plaque of the drug carrier. Moreover, we demonstrated the precise targeting of HACBC in vivo in apoE-/- mice by using HACBC coated with cy7. The mechanical stress-sensitive and CD44 receptor-targeted dual-response drug delivery system prepared by micellar composite hydrogel is the first application in the field of atherosclerosis, which provides a new method for diagnosing and treating atherosclerosis.Graphical abstractGraphical abstract for this article
       
  • Photothermal enhancement in sensitivity of lateral flow assays for
           detection of E-coli O157:H7
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Vahid Shirshahi, Seyed Nasrollah Tabatabaei, Shadie Hatamie, Reza SaberLateral flow immunoassay (LFA) is a well-known point-of-care technology for the detection of various analytes. However, low sensitivity and lack of quantitative results are some of its critical drawbacks. Here we report a photothermal enhanced lateral flow sensor on the basis of the photothermal properties of reduced graphene oxide (rGO) for the detection of E-coli O157:H7 as a model pathogen. The calibration curve of the photothermal method exhibited a linear range from 5 × 10⁵ to 5 × 10⁷ CFU/ml with a correlation coefficient of R2 = 0.96 and a regression equation of y = 8.1x-43 for standard bacteria solutions in phosphate buffer. The limit of detection was ∼5 × 10⁵ CFU/ml for standard bacteria solutions, which was a 10-fold enhancement in sensitivity compared to the qualitative results. Specificity experiments showed that the photothermal method can only detect the target bacteria among 6 types of bacteria strains. It was confirmed that the developed technique could be a highly potential method for the rapid detection field because it can provide fast quantitative results with improved sensitivity.Graphical abstractGraphical abstract for this article
       
  • Porous calcite CaCO3 microspheres: Preparation, characterization and
           release behavior as doxorubicin carrier
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Li Li, Yang Yang, Yirui Lv, Ping Yin, Ting LeiPorous CaCO3 microspheres are nowadays extensively used as a drug delivery system due to their excellent biocompatibility and degradability. However, the stability of vaterite CaCO3 microspheres is a major problem as a drug carrier. In this work, porous calcite CaCO3 microspheres are fabricated by calcination of gelatin-CaCO3 composite microspheres at 550 °C in air. The size and morphology of CaCO3 microspheres could be well regulated by the addition of gelatin. The structure of the as-prepared porous calcite CaCO3 microspheres is characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Compared with vaterite CaCO3 microspheres, porous calcite CaCO3 microspheres are more stable and exhibit good drug-loading and drug release properties for doxorubicin (DOX), an effective anticancer agent. This study provides an easy and novel approach to prepare stable porous calcite CaCO3 microspheres, which show promising potential as a carrier for DOX.Graphical abstractGraphical abstract for this article
       
  • Improved chemotherapeutic efficacy against resistant human breast cancer
           cells with co-delivery of Docetaxel and Thymoquinone by Chitosan grafted
           lipid nanocapsules: Formulation optimization, in vitro and in vivo studies
           
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Sobiya Zafar, Sohail Akhter, Iqbal Ahmad, Zubair Hafeez, M. Moshahid Alam Rizvi, Gaurav Kumar Jain, Farhan Jalees AhmadIn recent years, multi-targeted chemotherapeutic combinations have received considerable attention in solid tumor chemotherapy. Here, we optimized low-molecular-weight chitosan (CS)-grafted lipid nanocapsules (LNCs, referred to as CLNCs) for the co-delivery of docetaxel (DTX) and thymoquinone (THQ) to treat drug-resistant breast cancer. We first screened size reduction techniques (homogenization vs ultrasonication), and then the 33-Box-Behnken design was employed to determine optimal conditions of the final LNCs with the desired quality attributes. Uncoated LNCs had a particle size of 141.7 ± 2.8 nm (Polydispersity index, PdI: 0.17 ± 0.02) with entrapment efficiency (%EE) of 66.1 ± 3.5 % and 85.3 ± 3.1 % for DTX and THQ, respectively. The CS functionalization of LNCs improved the uptake and endosomal escape effect, and led to a significantly higher cytotoxicity against MCF-7 and triple-negative (MDA-MB-231) breast cancer cells. Furthermore, an enhanced antiangiogenic effect was observed with DTX- and THQ-carrying CLNCs in the Chick embryo chorioallantoic membrane (CAM) assay.Graphical abstractGraphical abstract for this article
       
  • Graphene aerogel nanoparticles for in-situ loading/pH sensitive releasing
           anticancer drugs
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Hossein Ayazi, Omid Akhavan, Mohammad Raoufi, Reyhaneh Varshochian, Najmeh Sadat Hosseini Motlagh, Fatemeh AtyabiFree polymer graphene aerogel nanoparticles (GA NPs) were synthesized by using reduction/aggregation of graphene oxide (GO) sheets in the presence of vitamin C (as a biocompatible reductant agent) at a low temperature (40 °C), followed by an effective sonication. Synthesis of GA NPs in doxorubicin hydrochloride (DOX)-containing solution results in the simultaneous synthesis and drug loading with higher performance (than that of the separately synthesized and loaded samples). To investigate the mechanism of loading and the capability of GA NPs in the loading of other drug structures, two groups of ionized (DOX, Amikacin sulfate and, d-glucosamine hydrochloride) and non-ionized (Paclitaxel (PTX)) drugs were examined. Furthermore, the relationship between the bipolar level of DOX solution (contributing to H-bonding of DOX and GO) and the amount of DOX loading was investigated. The DOX showed higher loading (>3 times) than PTX, as anticancer drugs. Since both DOX and PTX possess aromatic structures, the higher loading of DOX was assigned to its positive partial charge and ionized nature. Accordingly, other drugs (having positive partial charge and ionized nature, but no aromatic structure) such as Amikacin sulfate and d-glucosamine hydrochloride presented higher loading than PTX. These results indicated that although the π-π interactions induced by aromatic structures are important in drug loading, the electrostatic interaction of ionized drugs with GO (especially through H-bonding) is the dominant mechanism. DOX-loaded GANPs showed high pH-sensitive release (equivalent to the carrier weight) after 5 days, which can indicate benefits in tumor cell acidic microenvironments in-vivo.Graphical abstractGraphical abstract for this article
       
  • Hierarchy of interactions dictating the thermodynamics of real cell
           membranes: Following the insulin secretory granules paradigm up to
           fifteen-components vesicles
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Francesca Saitta, Marco Signorelli, Dimitrios FessasA fifteen-components model membrane that reflected the 80 % of phospholipids present in Insulin Secretory Granules was obtained and thermodynamic exploitation was performed, through micro-DSC, in order to assess the synergic contributions to the stability of a mixed complex system very close to real membranes. Simpler systems were also stepwise investigated, to complete a previous preliminary study and to highlight a hierarchy of interactions that can be now summarized as phospholipid tail unsaturation> phospholipid tail length> phospholipid headgroup> membrane curvature. In particular, Small Unilamellar Vesicles (SUVs) that consisted in phospholipids with different headgroups (choline, ethanolamine and serine), was step by step considered, following inclusion of sphingomyelins and lysophosphatidylcholines together with a more complete fatty acids distribution characterizing the phospholipid bilayer of the Insulin Secretory Granules. The inclusion of cholesterol was finally considered and the influence of three FFAs (stearic, oleic and elaidic acids) was investigated in comparison with simpler systems, highlighting the magnitude of the effects on such a detailed membrane in the frame of Type 2 Diabetes Mellitus alterations.Graphical abstractGraphical abstract for this article
       
  • Macrophage inflammatory and metabolic responses to graphene-based
           nanomaterials differing in size and functionalization
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Mónica Cicuéndez, Márcia Fernandes, Miguel Ayán-Varela, Helena Oliveira, María José Feito, Rosalía Diez-Orejas, Juan I. Paredes, Silvia Villar-Rodil, Mercedes Vila, M. Teresa Portolés, Iola F. DuarteThe preparation of graphene-based nanomaterials (GBNs) with appropriate stability and biocompatibility is crucial for their use in biomedical applications. In this work, three GBNs differing in size and/or functionalization have been synthetized and characterized, and their in vitro biological effects were compared. Pegylated graphene oxide (GO-PEG, 200–500 nm) and flavin mononucleotide-stabilized pristine graphene with two different sizes (PG-FMN, 200–400 nm and 100–200 nm) were administered to macrophages, chosen as cellular model due to their key role in the processing of foreign materials and the regulation of inflammatory responses. The results showed that cellular uptake of GBNs was mainly influenced by their lateral size, while the inflammatory potential depended also on the type of functionalization. PG-FMN nanomaterials (both sizes) triggered significantly higher nitric oxide (NO) release, together with some intracellular metabolic changes, similar to those induced by the prototypical inflammatory stimulus LPS. NMR metabolomics revealed that macrophages incubated with smaller PG-FMN displayed increased levels of succinate, itaconate, phosphocholine and phosphocreatine, together with decreased creatine content. The latter two variations were also detected in cells incubated with larger PG-FMN nanosheets. On the other hand, GO-PEG induced a decrease in the inflammatory metabolite succinate and a few other changes distinct from those seen in LPS-stimulated macrophages. Assessment of TNF-α secretion and macrophage surface markers (CD80 and CD206) further corroborated the low inflammatory potential of GO-PEG. Overall, these findings revealed distinct phenotypic and metabolic responses of macrophages to different GBNs, which inform on their immunomodulatory activity and may contribute to guide their therapeutic applications.Graphical abstractGraphical abstract for this article
       
  • An all-aqueous approach for physical immobilization of PEG-lipid microgels
           on organoid surfaces
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): M. Birgul Akolpoglu, Yasemin Inceoglu, Seda KizilelEmulsion-based generation of hydrogel particles has been widely explored for numerous applications in fields such as biomedical, food, and drug delivery. Water-in-water emulsion (w/w) is an organic solvent-free approach and exploits solely aqueous media to generate nano- or microparticles. This strategy is environment-friendly and favorable for biomedical applications where biocompatibility is the ultimate criterion. Hence, PEG-based microgels can be synthesized with desired size and functionality using w/w emulsion technique. To estimate the influence of emulsification parameters on size and stability of PEG-lipid microgels, optimizations using three independent input variables were carried out: (i) ultrasonication power, (ii) ultrasonication duration, and (iii) duration of light exposure. Physical immobilization of microgels on islet-organoids was achieved through hydrophobic interactions. Cell function and viability were assessed thoroughly after microgel immobilization. Microgel size is dependent on ultrasonication parameters and microgel stability is vastly determined by the duration of light exposure. Immobilization of microgels with 5 mM lipid moiety promoted coating of islet-organoids. Coated organoids retained their function and viability without significant adverse effects. This is important for understanding fundamental aspects of PEG-lipid microgels using w/w emulsion, useful for possible drug/gene delivery applications to increase treatment efficiency and ultimately lead to clinical translation of PEG microgels for biomedical applications.Graphical abstractGraphical abstract for this article
       
  • One-step electrodeposition of poly(m-aminobenzoic acid) membrane decorated
           with peptide for antifouling biosensing of Immunoglobulin E
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Shuai Wang, Yihui Ma, Yu Wang, Mingxia Jiao, Xiliang Luo, Min Cuim-Aminobenzoic acid (ABA) is one popular derivative of highly conductive monomer of aniline, which contains a carboxyl (COOH) group in its skeleton that is beneficial to various bio-interface and bio-analysis. Hence, Poly(m -aminobenzoic acid) (PABA) membrane was firstly electrochemical deposited onto bare electrode surface using a straightforward cyclic voltammetry (CV) method. PABA membrane exhibited excellent electrochemical stability and apparent wrinkle morphology that could effectively enhance response signal and immensely increase specific surface area of electrode. Next, PABA membrane was decorated with well-designed hairpin aptamer and preferred antifouling peptide in sequence to construct a two-layer architectural bio-interface that could present both sensitive target recognition capability and excellent antifouling ability. Benefiting from the electrodeposited PABA membrane to enhance electrochemical response signal, the developed biosensor performed excellent sensitivity toward target protein, meanwhile, associated with good selectivity and reproducibility attributing to the favored peptide that was able to decline nonspecific protein adsorption and improve target recognition.Graphical abstractGraphical abstract for this article
       
  • Enhanced corrosion resistance and bioactivity of Mg alloy modified by
           Zn-doped nanowhisker hydroxyapatite coatings
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Wuchao Zhou, Zhenrong Hu, Taolei Wang, Guangzheng Yang, Weihong Xi, Yanzi Gan, Wei Lu, Jingzhou HuIn this work, Zn is doped into a hydroxyapatite coating on the surface of ZK60 magnesium alloys using a one-pot hydrothermal method to obtain a corrosion-resistant implant with abilities of osteogenic differentiation and bacterial inhibition. With the addition of Zn, the morphology changes with a nanowhisker structure appearing on the coating. Electrochemical measurements show that the nanowhisker hydroxyapatite coating provides a high corrosion resistance. Compared with hydroxyapatite coating, the nanowhisker coating not only effectively inhibits bacteria, but also promotes the adhesion and differentiation of rat bone marrow mesenchymal stem cells at appropriate Zn concentrations. In conclusion, a novel nanowhisker structure prepared by a single variable Zn doping can significantly improve the corrosion resistance and biological activity of hydroxyapatite coatings.Graphical abstractGraphical abstract for this article
       
  • Corrigendum to “Encapsulation of an endostatin peptide in liposomes:
           Stability, release, and cytotoxicity study” [Colloids Surf. B
           Biointerfaces 185 (October) (2019) 110552]
    • Abstract: Publication date: February 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 186Author(s): Nastaran Rezaei, Faramarz Mehrnejad, Zahra Vaezi, Mosslim Sedghi, S. Mohsen Asghari, Hossein Naderi-Manesh
       
  • Study on a novel antibacterial light-cured resin composite containing
           nano-MgO
    • Abstract: Publication date: Available online 7 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhongyuan Wu, Haiping Xu, Wei Xie, Meimei Wang, Cunjin Wang, Cheng Gao, Fang Gu, Jie Liu, Jing FuA new type of photocurable resin composite containing nano-MgO was synthesized in order to reduce the occurrence of secondary caries. Different mass ratios (0%, 1%, 2%, 4%, 8%) of nano-MgO were added into resin composites. The antibacterial properties of nano-MgO powder and modified resin composites against Streptococcus mutans (S. mutans) were detected by antibacterial ring test and film contact test, respectively. Compressive strength (CS) and wear resistance were determined by a universal testing machine and an abrasion test machine. The results indicated that antibacterial activity and wear resistance of resin composites containing nano-MgO were superior to the control group (p 
       
  • Physical and biological effects of paclitaxel encapsulation on
           
    • Abstract: Publication date: Available online 31 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Caroline Mari Ramos Oda, Antônio Augusto Malfatti-Gasperini, Angelo Malachias, Gwenaelle Pound-Lana, Vanessa Carla Furtado Mosqueira, Renata Salgado Fernandes, Mônica Cristina de Oliveira, André Luis Branco de Barros, Elaine Amaral LeiteSimple size observations of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-mPEG2000) polymeric micelles (PM) with different compositions including or not paclitaxel (PTX) are unable to evidence changes on the nanocarrier structure. In such system a detailed characterization using highly sensitive techniques such as X-ray scattering and asymmetric flow field flow fractionation coupled to multi-angle laser light scattering and dynamic light scattering (AF4-MALS-DLS) is mandatory to observe effects that take place by the addition of PTX and/or more lipid-polymer at PM, leading to complex changes on the structure of micelles, as well as in their supramolecular organization. SAXS and AF4-MALS-DLS suggested that PM can be found in the medium separately and highly organized, forming clusters of PM in the latter case. SAXS fitted parameters showed that adding the drug does not change the average PM size since the increase in core radius is compensated by the decrease in shell radius. SAXS observations indicate that PEG conformation takes place, changing from brush to mushroom depending on the PM composition. These findings directly reflect in in vivo studies of blood clearance that showed a longer circulation time of blank PM when compared to PM containing PTX.Graphical Graphical abstract for this article
       
  • Construction and application of targeted drug delivery system based on
           hyaluronic acid and heparin functionalised carbon dots
    • Abstract: Publication date: Available online 31 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Qianqian Duan, Lan Ma, Boye Zhang, Yixia Zhang, Xiaoning Li, Tao Wang, Wendong Zhang, Yi Li, Shengbo SangThrombosis is a main complication of cancer. It can increase the mortality of cancer patients. Therefore, the anticoagulant heparin (Hep) as an adjuvant therapy was introduced to the drug delivery system based on doxorubicin hydrochloride (DOX)-carbon dots (CDs)-Hyaluronic acid (HA), which obviously enhanced the blood compatibility of the system. Drug release process of the CDs-HA-Hep/DOX system was dual-responsive by HA and pH value. Results of in vitro MTT and scratch tests demonstrated that the drug delivery system could targetedly inhibit growth and migration of cancerous cells. In addition, the system allows visual tracking of the drug based on fluorescence of CDs.Graphical abstractGraphical abstract for this article
       
  • Determination of the bioavailability of zinc oxide nanoparticles using
           ICP-AES and associated toxicity
    • Abstract: Publication date: Available online 30 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): S Sudhakaran, SS Athira, Suresh Babu S, HK Varma, PV MohananAdvancement in nanotechnology has brought abundant number of products and materials in multiple fields including biomedicine owing to their unique physico-chemical properties. This further necessitates toxicity assessment of nanoparticles (NPs) before they are employed for product fabrication, medicinal, environmental or industrial purposes. Zinc oxide nanoparticles (ZnONPs) belong to the category of metal oxide NPs and hold quite a lot of possibilities to be applied in aforementioned scenarios. Present study addresses the probable outcomes of bio-nano interaction of ZnONPs with healthy adult Wistar rats. Sphere head shaped ZnONPs were synthesized via wet chemical method. Physico-chemical characterization was performed using number of sophisticated techniques including HR-TEM, Zeta potential analysis, TGA and XRD. Size of the particles was found to be 43 nm and ensured homogenous distribution with high purity. For in vivo studies, as synthesized NPs were administered into rats via intravenous (i.v.) and intraperitoneal (i.p.) routes. Animals were sacrificed on 3rd, 14th and 21st day of exposure. Metabolically relevant tissues like brain, liver, kidneys and spleen were isolated and analyzed for different parameters like gross pathology, haematology, neurotoxicity, target organ toxicity, immunotoxicity etc. Results suggests that ZnONPs did not elicit significant toxic responses in rat except a few anomalies with histology, ion content and antioxidant system within liver; thereby confirming potent hepatotoxicity. Hence the study recommends adopting surface functionalization strategies for reducing toxic response of ZnONPs during various application rationales.Graphical abstractGraphical abstract for this article
       
  • Performance of Polyvinyl Pyrrolidone-Isatis Root Antibacterial Wound
           Dressings Produced in situ by Handheld Electrospinner
    • Abstract: Publication date: Available online 28 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Wen-Hao Dong, Xiao-Ju Mou, Guo-Sai Liu, Xiao-Wei Huang, Xu Yan, Xin Ning, Stephen J. Russell, Yun-Ze LongAntibacterial dressings are an increasingly important tool for the prevention and management of wound infections, particularly in light of concerns surrounding conventional drug-resistant antibiotics. Handheld electrospinning devices provide opportunities for the rapid application of antibacterial dressing materials to wounds, but spinning formulations need to be compatible with live biological surfaces. We report the development of a new antibacterial formulation compatible with handheld electrospinning, and its manufacture directly on a wound site. Nanofibrous dressing mats were produced from polyvinyl pyrrolidone (PVP) containing isatis root (Indigowoad root or Ban-Lan-Gen), a traditional Chinese medicine, commonly used for the treatment of infectious disease. The resulting wound dressing mats of PVP/isatis root exhibited well-defined fibrous structures and excellent surface wetting, and permeability characteristics. The presence of isatis root conferred antibacterial activity against gram negative and gram positive strains. Moreover, in a Kunming mouse skin injury model, direct electrospinning of PVP/isatis root formulations on to wound sites produced near complete wound closure after 11 days and epidermal repair in histological studies.Graphical abstractGraphical abstract for this article
       
  • Retinoic acid-loaded solid lipid nanoparticles surrounded by chitosan film
           support diabetic wound healing in in vivo study
    • Abstract: Publication date: Available online 27 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Valquíria T. Arantes, André A.G. Faraco, Frederico B. Ferreira, Cleida A. Oliveira, Elisângela Martins-Santos, Puebla Cassini-Vieira, Lucíola S. Barcelos, Lucas A.M. Ferreira, Gisele A.C. GoulartABSTRACTRepair of tissue damaged in diabetic wounds is essential to minimize the cases of amputation of the limbs in millions of diabetic people around the world. Although the all-trans retinoic acid (ATRA) is described as a potential wound healing agent, however its effects are controversial due to adverse reactions that may impair the wound healing during the treatment schedules. Our aim was to design and characterize an ATRA-loaded solid lipid nanoparticles surrounded by chitosan film to promote an ATRA controlled release and to evaluate its effectiveness in promoting wound healing in a diabetic mouse model. The SLN-ATRA were developed using biocompatible lipids without using organic solvent. The SLN-ATRA had high drug entrapment efficiency (98.0%) and low polydispersity index (PDI) and average diameter, respectively, 0.24 ± 0.02 and 83.0 ± 6 nm. The transmission electron microscope (TEM) image presented that the SLN-ATRA were homogeneous in size and had spherical structures. The incorporation of SLN-ATRA in the chitosan films propitiated a homogeneous distribution of the drug and a controlled drug release. Furthermore, in vivo assay proved that chitosan films containing SLN-ATRA accelerated the closure of wounds of diabetic mice when compared to the control chitosan films without ATRA. SLN-ATRA chitosan films also reduced leukocyte infiltrate in the wound bed, improved collagen deposition, and reduced scar tissue. No sign of skin irritation was observed. These results indicated that SLN-ATRA surrounded in chitosan films are a promising candidate to treat diabetic wounds, improving tissue healing.Graphical abstractGraphical abstract for this article
       
  • One step synthesis of antimicrobial peptide protected silver
           nanoparticles: the core-shell mutual enhancement of antibacterial activity
           
    • Abstract: Publication date: Available online 3 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jingyu Gao, Heiya Na, Ruibo Zhong, Ming Yuan, Jun Guo, Lijiao Zhao, Yu Wang, Liping Wang, Feng ZhangOver the past few decades, the overuse of antibiotics has led to the emergence of resistant bacteria and environmental issues. Both silver nanoparticles (AgNPs) and antimicrobial peptides (AMPs) hold potential to replace antibiotics. Combining both AMPs and AgNPs into a composite material may create novel properties such as enhanced antibacterial activity, lower cytotoxicity and favorable stability in aqueous solution. We designed a 13 amino acid peptide (in short, P-13) with two functional regions: one is for antibacterial activity, and the other for reducing and stabilizing AgNPs with containing cysteine (C) residues in its C-terminus. With a single step reaction, we have successfully synthesized P-13 protected AgNPs (P-13@AgNPs) with a hydrodynamic diameter of about 11 nm. In the preliminary antibacterial activity assay, the minimum inhibitory concentrations (MICs) of P-13@AgNPs were up to 7.8 μg/mL against E. coli, S. aureus and B. pumilus, and 15.6 μg/mL against P. aeruginosa. Moreover, Flow cytometry analysis of E. coli, S. aureus, P. aeruginosa and B. pumilus show that the mortality of the strains reached 96%, 96%, 91% and 90%, respectively. The cytotoxicity of AgNPs was reduced dramatically after protected by P-13, and P-13 was favorable for the stability of the AgNPs solution. We believe this work could set up an example to make the best use of the individual material’s properties to produce novel nanocomposites with better antibacterial activity.Graphical abstractGraphical abstract for this article
       
  • Dual-drugs delivery in Solid Lipid Nanoparticles for the treatment of
           Candida albicans mycosis
    • Abstract: Publication date: Available online 3 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): C. Carbone, V. Fuochi, A. Zielińska, T. Musumeci, E.B. Souto, A. Bonaccorso, C. Puglia, G. Petronio Petronio, P.M. FurneriNowadays, a combinatorial drug delivery system that simultaneously transports two or more drugs to the targeted site in a human body, also recognized as a dual-drugs delivery system, represents a promising strategy to overcome drug resistance. Solid lipid nanoparticles loaded with clotrimazole (CLZ) and alphalipolic acid (ALA), considered as an effective agent in the reduction of reactive oxygen species, can enhance anti-infective immunity being proposed as a non-toxic and mainly non-allergic dual-drugs delivery system. In this study, uncoated and cationic CLZ-ALA-loaded SLN were prepared and compared. Suspensions with a narrow size distribution of particles of mean size below 150 nm were obtained, having slight negative or highly positive zeta potential values, due to the presence of the cationic lipid, which also increased nanoparticles stability, as confirmed by Turbiscan® results. Calorimetric studies confirmed the rationale of separately delivering the two drugs in a dual-delivery system. Furthermore, they confirmed the formation of SLN, without significant variation in presence of the cationic lipid. In vitro release studies showed a prolonged drug release without the occurrence of any burst effect. In vitro studies performed on 25 strains of Candida albicans showed the antimicrobial drug activity was not altered when it was loaded into lipid nanoparticles. The study has proved the successfully encapsulation of CLZ and ALA in solid lipid nanoparticles that may represent a promising strategy to combine ALA protective effect in the treatment with CLZ.Graphical abstractGraphical abstract for this article
       
  • Interaction between soyasaponin and soy β-conglycinin or glycinin:
           air-water interfacial behavior and foaming property of their mixtures
    • Abstract: Publication date: Available online 3 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Lijie Zhu, Peng Yin, Tianyu Xie, Xiuying Liu, Lina Yang, Shengnan Wang, Jun Li, He LiuThe interaction between soyasaponin and soy β-conglycinin (7S) or glycinin (11S), adsorption of their mixtures at air-water interface, and foaming properties of the mixed system were investigated in this study. Fluorescence spectroscopy results showed that there was a weak binding of soyasaponin with 7S or 11S in bulk solutions, leading to the conformational changes of protein by nonspecific hydrophobic interactions. Dynamic surface properties of soyasaponin-7S/11S mixtures indicated that the composite layers formed via their weak interactions due to the synergy of reducing surface tension and the plateau of elasticity at the interface. Most mixtures represented high foam forming ability and stability except 0.2% soyasaponin mixture, which could be a consequence that the surface behavior was dominated by soyasaponin under this concentration, and low surface elasticity lead to a less stable interfacial film. Overall, foamability of soyasaponin-7S mixtures were better than 11S ones. All data of this work was helpful to understand air-water behaviors of soyasaponin-7S/11S mixtures. This mixed system has shown good potential for further foam related industrial applications.Graphical sGraphical abstract for this articleInteraction between soyasaponin and soy β-conglycinin or glycinin at air-water interface.
       
  • A fullerene based hybrid nanoparticle facilitates enhanced photodynamic
           therapy via changing light source and oxygen consumption
    • Abstract: Publication date: Available online 2 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yunwei Yan, Kaixiang Zhang, Huiling Wang, Wei Liu, Zhenzhong Zhang, Junjie Liu, Jinjin ShiRecently, fullerene (C60) has been widely used as a nano photosensitizer (PS) for tumor related photodynamic therapy (PDT). However, current PDT based on C60 is severely restricted by the visible light source (shallow tissue penetrating depth) and oxygen dependent (tumor hypoxia). Therefore, taking advantages of the surface plasmon resonance (SPR) effect of gold nanoparticles (GNPs) and “electronic sponge” property of C60, a C60 based hybrid nanostructured photosensitizer (C60@GNPs) with high light stability, near infrared light (NIR) excitation, and oxygen non-dependent properties was rational designed according to the mechanism of PDT. Compared with C60, after GNPs in-situ synthesis, the PDT mechanism of C60@GNPs changed from type II to type I, and the main product of PDT changed from singlet oxygen to hydroxyl radicals. Furthermore, C60@GNPs hybrid could efficiently generate hydroxyl radicals under NIR light excitation even in the hypoxia condition. These results suggest that C60@GNPs hybrid has a great potential for in vivo PDT applications.Graphical abstractGraphical abstract for this article
       
  • Resveratrol-loaded chitosan–γ-poly(glutamic acid) nanoparticles:
           
    • Abstract: Publication date: Available online 2 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Joo Hee Chung, Ji-Soo Lee, Hyeon Gyu LeeABSTRACTThe objective of this study was to investigate the effects of the particle size of resveratrol (RSV)-loaded nanoparticles (NPs) on their solubility and stability and to optimize their preparation conditions for their solubility and stability.RSV-loaded NPs were prepared using chitosan and γ-poly(glutamic acid) (γ-PGA). Although the solubility and stability of RSV have been significantly increased using chitosan/γ-PGA nanoencapsulation, as the NP size decreased, the solubility increased, but the stability decreased. In order to understand the interrelationship of particle size, solubility, and stability, the target values of RSV solubility and ultraviolet (UV) stability for the aforementioned optimization were determined at two levels: solubility> 153 μg/mL, UV stability> 12% (S153U12) and solubility> 150 μg/mL, UV stability> 18% (S150U18). The S150U18-NPs (258 nm) showed a significantly higher UV stability and tyrosinase inhibition activity against UVA than S153U12-NPs (87 nm) (p 
       
  • Size effect of curcumin nanocrystals on dissolution, airway mucosa
           penetration, lung tissue distribution and absorption by pulmonary delivery
           
    • Abstract: Publication date: Available online 2 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yuan He, Yingmin Liang, Judith Choi Wo Mak, Yonghong Liao, Ting Li, Ru Yan, Hai-Feng Li, Ying ZhengNanocrystals (NCs) have been introduced for use in pulmonary delivery in recent decades. Although the deposition and bioavailability have been extensively studied, little is known about the biofate, which influences the drug release and absorption process of NCs. In this study, we fabricated three different sized curcumin NCs by adjusting the parameters of mill machine using a wet milling method and studied the size effect on pulmonary absorption. The small nanocrystals (NC-S, 246.16 ± 21.98 nm) exhibited a faster dissolution rate and higher diffusion percentage in vitro compared with middle (NC-M, 535.26 ± 50.33 nm) and large nanocrystals (NC-L, 1089.53 ± 194.34 nm). Multiple particle tracking experiments revealed that NC-S had larger mean squared displacement during diffusion in simulated mucus of 0.5% hydroxyethyl cellulose solution. Moreover, enhanced cellular uptake and transport efficiency were achieved by NC-S in Calu-3 cells and an air-liquid interface culturing model. NCs were mainly absorbed in the dissolved drug form, as assessed by using the Förster resonance energy transfer (FRET) technique. In vivo lung retention and distribution revealed that few smaller sized nanocrystals were retained in the lung after intratracheal administration. The pharmacokinetic study showed that the AUC(0-t) values of small sized nanocrystals were 1.75- and 3.32-fold greater than NC-M and NC-L, respectively. In conclusion, this study demonstrated that smaller sized nanocrystals were more easily absorbed into the blood system by increasing the dissolution rate.Graphical abstractGraphical abstract for this article
       
  • Micro-Patterned SU-8 Cantilever Integrated with Metal Electrode for
           Enhanced Electromechanical Stimulation of Cardiac Cells
    • Abstract: Publication date: Available online 28 November 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nomin-Erdene Oyunbaatar, Arunkumar Shanmugasundaram, Yun-Jin Jeong, Bong-Kee Lee, Eung-Sam Kim, Dong-Weon LeeOver the past few years, cardiac tissue engineering has undergone tremendous progress. Various in vitro methods have been developed to improve the accuracy in the result of drug-induced cardiac toxicity screening. Herein, we propose a novel SU-8 cantilever integrated with an electromechanical-stimulator to enhance the maturation of cultured cardiac cells. The simultaneous electromechanical stimulation significantly enhances the contraction force of the cardiomyocytes, thereby increasing cantilever displacement. Fluorescence microscopy analysis was performed to confirm the improved maturation of the cardiomyocytes. After the initial experiments, the contractile behaviors of the cultured cardiomyocytes were investigated by measuring the mechanical deformation of the SU-8 cantilever. Finally, the proposed electromechanical-stimulator-integrated SU-8 cantilever was used to evaluate the adverse effects of different cardiac vascular drugs, i.e., verapamil, lidocaine, and isoproterenol, on the cultured cardiomyocytes. The physiology of the cardiac-drug-treated cardiomyocytes was examined with and without electrical stimulation of the cardiomyocytes. The experimental results indicate that the proposed cantilever platform can be used as a predictive assay system for preliminary cardiac drug toxicity screening applications.Graphical abstractGraphical abstract for this articleHighlights
       
  • Histone H2A-peptide-hybrided upconversion mesoporous silica nanoparticles
           for bortezomib/p53 delivery and apoptosis induction
    • Abstract: Publication date: Available online 28 November 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jiamin Rong, Pengcheng Li, Yakun Ge, Hongling Chen, Jie Wu, Renwen Zhang, Jun Lao, Dawei Lou, Yuanxin ZhangThe design and development of advanced gene/drug codelivery nanocarrier with good biocompatibility for cancer gene therapy is desirable. Herein, we reported a gene delivery nanoplatform to synergized bortezomib (BTZ) for cancer treatment with histone H2A-hybrided, upconversion luminescence (UCL)-guided mesoporous silica nanoparticles [UCNPs(BTZ)@mSiO2-H2A]. The functionalization of H2A on the surface of UCNPs(BTZ)@mSiO2 nanoparticles realized the improvement of biocompatibility and enhancement of gene encapsulation and transfection efficiency. More importantly, then UCNPs(BTZ)@mSiO2-H2A/p53 induced specific and efficient apoptotic cell death in p53-null cancer cells and restored the functional activity of tumor suppressor p53 by the success of co-delivery of BTZ/p53. Moreover, the transfection with UCNPs(BTZ)@mSiO2-H2A/p53 in p53-deficient non-small cell lung cancer cells changed the status of p53 and substantially enhanced the p53-mediated sensitivity of encapsulated BTZ inside the UCNPs(BTZ)@mSiO2/p53. Meanwhile, core-shell structured mesoporous silica nanoparticles UCNPs@mSiO2 as an UCL agent can detect the real-time interaction of nanoparticles with cells and uptake/penetration processes. The results here suggested that the as-developed UCNPs(BTZ)@mSiO2-H2A/p53 nanoplatform with coordinating biocompatibility, UCL image, and sustained release manner might be desirable gene/drug codelivery nanocarrier for clinical cancer therapy.Graphical abstractGraphical abstract for this article
       
  • Structure Retention of Silica Gel-Encapsulated Bacteriorhodopsin in Purple
           Membrane and in Lipid Nanodiscs
    • Abstract: Publication date: Available online 27 November 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sukriti Gakhar, Subhash H. Risbud, Marjorie L. LongoThe integral membrane protein, bacteriorhodopsin (BR) was encapsulated in sol-gel derived porous silica gel monoliths in native purple membrane (BR-PM) and synthetic lipid nanodisc (BR nanodisc) environments. BR nanodiscs were synthesized by solubilizing purple membrane in discoidal phospholipid bilayer stabilized by amphipathic Styrene-Maleic Acid (SMA) copolymer. UV-Visible absorbance spectroscopy and dynamic-light scattering indicated the formation of BR monomers solubilized in lipid nanodiscs 10.2 ± 0.7 nm in average diameter. Fluorescence and absorbance spectroscopic techniques were utilized to probe conformational, environmental, and rotational changes associated with the tryptophan residues and the covalently-bound retinal moiety of BR upon entrapment in the silica matrix. We show that the immobilized BR in both membrane environments retained its bound retinal cofactor and the ability of the cofactor to undergo conformational changes upon light illumination necessary for BR’s activity as a proton transporter. For purple membrane fragments, the results indicated that the local pH in the pores around BR after encapsulation was important for its stability at temperatures higher than 50 °C. Under the same buffering conditions, retinal was released from silica-encapsulated BR-PM and BR nanodiscs beginning at 80 °C (without a conformational change) and 50 °C (with a conformational change), respectively, reflecting differences in protein-protein (trimeric vs. monomeric) and protein-lipid interactions.Graphical Graphical abstract for this article
       
  • Amphiphilic p-Sulfonatocalix[6]arene Based Self-assembled Nanostructures
           for Enhanced Clarithromycin Activity Against Resistant Streptococcus
           Pneumoniae
    • Abstract: Publication date: Available online 27 November 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Imdad Ali, Muhammad Imran, Salim Saifullah, Han-Wen Tian, Dong-Sheng Guo, Muhammad Raza ShahAmphiphilic calixarenes are preferred to generate nano-cargos for drugs due to their stability, possibilities for modification and intrinsic host cavities. Here we are reporting the synthesis of amphiphilic calixarene and its evaluation as drug delivery system. Water soluble amphiphilic p-sulfonatocalix[6]arene was synthesized through sulfonationand lipophilic conjugation on itsupper and lower rims respectively. The synthesized amphiphile self-assembled into nanostructures in the presence of Clarithromycin and FITC as model hydrophobic drugs followed by a wide range of characterization. Clarithromycin loaded self-assembled nanostructures was screened for its bactericidal potential in resistant S. pneumonia through various in-vitro assays. The amphiphilic calixarene self-assembled into polydispersed nanostructures with 136.45 ± 2.41 nm mean diameter and -49.93 ± 0.35 mV surface charges. The amphiphile was capable to load Clarithromycin (57.54 ± 1.88%) and fluorescent dye and was highly stable. Clarithromycin loaded nanostructures revealed significant biofilm and bacterial growth inhibition and cell destruction properties. Results authenticate calixarene amphiphile as an efficient nano-carrier for improving Clarithromycin efficacy.Graphical Graphical abstract for this article
       
 
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