American Journal of Biochemistry and Molecular Biology
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Open Access journal
ISSN (Print) 2150-4210 - ISSN (Online) 2150-4253
Published by Academic Journals Inc [4 journals]
- Effect of Consumption of Corchorus olitorius L., in Carbon
Tetrachloride-Induced Liver Damage in Male Wistar Rats
Abstract: Carbon tetrachloride (CCl4) has been extensively used in experimental models to demonstrate its hepatotoxic potential. Humans are often exposed to it where it is used in petrol additives, refrigerants, catalyst in polymer formation and in pesticides. In this study, the effect of leaves of Corchorus olitorius L. in CCl4-induced liver damage in male wistar rats was assessed using alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), plasma total protein, superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), reduced glutathione (GSH), Packed Cell Volume (PCV), hemoglobin (Hb) and White Blood Cell (WBC) as well as histological assay. Thirty five male wistar rats distributed into seven groups of five rats each were used in this study. The 1 mL kg-1 body weight of CCl4 was administered orally thrice in a week to hepatotoxic groups. Animals in all the groups were either fed control diet or C. olitorius-supplemented diets (COSD). It was observed from the result of this study that exposure to CCl4 and Corchorus olitorius L., produced a significant increase (p0.05) in AST, ALP and SOD activities and a significant decrease (p0.05) and microscopic examination showed severe histological damage in hepatotoxic groups fed with C. olitorius-supplemented diet. These observations indicate that regular consumption of unprocessed C. olitorius L., may further enhance the hepatotoxic potential of CCl4 in humans.
PubDate: 03 January, 2015
- Antiplasmodial and Antioxidant Activities of Saye: A Traditional Herbal
Remedy for Malaria
Abstract: Saye is an antimalarial recipe containing Cochlospermum planchonii Hook. F. (Cochlospermaceae), Phyllanthus amarus Schumach and Thonn (Euphorbiaceae) and Cassia alata L. (Fabaceae). This study assessed the antiplasmodial and antioxidant activity of the aqueous extracts of the individual plants and their combinations. Extracts were assessed on Plasmodium berghei infected mice according to the 4 day suppressive test, for their total phenol contents according to Folin-Ciocalteu method and their antioxidant potential by FRAP assay and by the inhibition of ion induced lipid peroxidation in rat liver homogenate. Macerated extracts from Cochlospermum planchonii, Phyllantus amarus and Cassia alata gave 50.6, 46.3 and 44.9% inhibition of the parasites at 100 mg kg-1 body weight (b.wt.), respectively. At 250 mg kg-1 b.wt. the decocted extract of Cochlospermum planchoii gave 54% inhibition. Decocted extracts of the combinations Cassia alata+Phyllanthus amarus (2:1) and Phyllanthus amarus+Cochlospermum planchonii (2:1) reduced mice parasitemia by about 20-30% at 100 mg kg-1 b.wt. At the same dose, a 4.9-15.9% inhibition was observed with the decocted extract of Cassia alata+Cochlospermum planchonii (1:1) and the whole Saye but a higher effect of 43.7 to 50.3% was observed at 250 mg kg-1 b.wt. Phyllantus amarus extract exhibited the highest total phenol content (294 μg TAEs/mg). The highest content in flavonoids 43 μg QE/mg and the highest inhibition of lipid peroxidation (22.56%) were found for Cassia alata+Phyllanthus amarus (2:1) and the best reducing power (41.38 μmol TE/mg) for Saye. Saye appears as promising antioxidant and could be used as preventive agent in oxidative stress diseases such as malaria.
PubDate: 03 January, 2015
- Effects of Oral Administration of Garcinia kola Seeds on Haematological
and Defence Parameters of Diabetic Rats
Abstract: The effects of oral administration of Garcinia kola seeds on haematological and defence parameters of diabetic rats were investigated. Thirty acclimatized wistar rats weighing between 240-250 g were divided into six groups of five animals per group (n = 5). The first two groups of rats; non-diabetic control and non-diabetic treated groups orally received normal saline and 600 mg kg-1 b.wt. of Garcinia kola seed powder (GKP), respectively. The last four groups which were made diabetic by intra-peritoneal injection of alloxan monohydrate had one diabetic control that orally received normal saline and three diabetic groups that got 300, 600, 900 mg kg-1 b.wt. of GKP. The GKP was then given twice daily for 21 days after which the animals were sacrificed, blood collected by cardiac puncture for glucose and haematological analysis. Results showed that GKP significantly reduced blood glucose, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and lymphocyte of diabetic rats. It significantly increased RBC, WBC, PCV, neutrophil and monocyte of diabetic treated rats. This study therefore depicts GKP as a hypoglycaemic agent with potential to normalize aberrant haematological and defence parameters associated with diabetes.
PubDate: 03 January, 2015