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  Subjects -> CHEMISTRY (Total: 894 journals)
    - ANALYTICAL CHEMISTRY (55 journals)
    - CHEMISTRY (628 journals)
    - CRYSTALLOGRAPHY (21 journals)
    - ELECTROCHEMISTRY (28 journals)
    - INORGANIC CHEMISTRY (43 journals)
    - ORGANIC CHEMISTRY (48 journals)
    - PHYSICAL CHEMISTRY (71 journals)

CHEMISTRY (628 journals)                  1 2 3 4 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 14)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 28)
ACS Catalysis     Hybrid Journal   (Followers: 44)
ACS Chemical Neuroscience     Hybrid Journal   (Followers: 22)
ACS Combinatorial Science     Hybrid Journal   (Followers: 23)
ACS Macro Letters     Hybrid Journal   (Followers: 26)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 42)
ACS Nano     Hybrid Journal   (Followers: 303)
ACS Photonics     Hybrid Journal   (Followers: 14)
ACS Symposium Series     Full-text available via subscription  
ACS Synthetic Biology     Hybrid Journal   (Followers: 24)
Acta Chemica Iasi     Open Access   (Followers: 5)
Acta Chimica Slovaca     Open Access   (Followers: 2)
Acta Chimica Slovenica     Open Access   (Followers: 1)
Acta Chromatographica     Full-text available via subscription   (Followers: 8)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 7)
Acta Scientifica Naturalis     Open Access   (Followers: 3)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 8)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 9)
Adsorption Science & Technology     Open Access   (Followers: 6)
Advanced Functional Materials     Hybrid Journal   (Followers: 60)
Advanced Science Focus     Free   (Followers: 5)
Advances in Chemical Engineering and Science     Open Access   (Followers: 73)
Advances in Chemical Science     Open Access   (Followers: 18)
Advances in Chemistry     Open Access   (Followers: 23)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 19)
Advances in Drug Research     Full-text available via subscription   (Followers: 25)
Advances in Environmental Chemistry     Open Access   (Followers: 7)
Advances in Enzyme Research     Open Access   (Followers: 10)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 9)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 16)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 12)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 26)
Advances in Nanoparticles     Open Access   (Followers: 15)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 17)
Advances in Polymer Science     Hybrid Journal   (Followers: 45)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Science and Technology     Full-text available via subscription   (Followers: 12)
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 3)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 8)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Al-Kimia : Jurnal Penelitian Sains Kimia     Open Access  
Alchemy : Journal of Chemistry     Open Access   (Followers: 3)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 22)
American Journal of Chemistry     Open Access   (Followers: 32)
American Journal of Plant Physiology     Open Access   (Followers: 11)
American Mineralogist     Hybrid Journal   (Followers: 16)
Anadolu University Journal of Science and Technology A : Applied Sciences and Engineering     Open Access  
Analyst     Full-text available via subscription   (Followers: 38)
Angewandte Chemie     Hybrid Journal   (Followers: 179)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 256)
Annales UMCS, Chemia     Open Access   (Followers: 1)
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 5)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 4)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 9)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 2)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 9)
Applied Spectroscopy     Full-text available via subscription   (Followers: 24)
Applied Surface Science     Hybrid Journal   (Followers: 32)
Arabian Journal of Chemistry     Open Access   (Followers: 6)
ARKIVOC     Open Access   (Followers: 1)
Asian Journal of Biochemistry     Open Access   (Followers: 3)
Asian Journal of Chemistry and Pharmaceutical Sciences     Open Access  
Atomization and Sprays     Full-text available via subscription   (Followers: 4)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 3)
Avances en Quimica     Open Access  
Biochemical Pharmacology     Hybrid Journal   (Followers: 11)
Biochemistry     Hybrid Journal   (Followers: 376)
Biochemistry Insights     Open Access   (Followers: 6)
Biochemistry Research International     Open Access   (Followers: 6)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 11)
Bioinspired Materials     Open Access   (Followers: 5)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access   (Followers: 2)
Biomacromolecules     Hybrid Journal   (Followers: 22)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 10)
Biomedical Chromatography     Hybrid Journal   (Followers: 6)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 5)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 138)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 87)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 18)
Biosensors     Open Access   (Followers: 2)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 1)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 24)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 3)
Cakra Kimia (Indonesian E-Journal of Applied Chemistry)     Open Access  
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 2)
Canadian Journal of Chemistry     Hybrid Journal   (Followers: 11)
Canadian Mineralogist     Full-text available via subscription   (Followers: 6)
Carbohydrate Research     Hybrid Journal   (Followers: 26)
Carbon     Hybrid Journal   (Followers: 70)
Catalysis for Sustainable Energy     Open Access   (Followers: 8)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 7)
Catalysis Science and Technology     Free   (Followers: 8)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 11)
Cellulose     Hybrid Journal   (Followers: 9)
Cereal Chemistry     Full-text available via subscription   (Followers: 5)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 1)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 22)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 75)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 27)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Hybrid Journal   (Followers: 22)
Chemical Reviews     Hybrid Journal   (Followers: 204)
Chemical Science     Open Access   (Followers: 27)
Chemical Technology     Open Access   (Followers: 32)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 5)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 57)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 24)
ChemInform     Hybrid Journal   (Followers: 8)
Chemistry & Biodiversity     Hybrid Journal   (Followers: 7)
Chemistry & Biology     Full-text available via subscription   (Followers: 33)
Chemistry & Industry     Hybrid Journal   (Followers: 8)
Chemistry - A European Journal     Hybrid Journal   (Followers: 166)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 16)
Chemistry and Materials Research     Open Access   (Followers: 21)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Open Access   (Followers: 3)
Chemistry Letters     Full-text available via subscription   (Followers: 45)
Chemistry of Materials     Hybrid Journal   (Followers: 266)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 9)
Chemistry World     Full-text available via subscription   (Followers: 20)
Chemistry-Didactics-Ecology-Metrology     Open Access   (Followers: 1)
ChemistryOpen     Open Access   (Followers: 1)
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 3)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 15)
Chemosensors     Open Access  
ChemPhysChem     Hybrid Journal   (Followers: 12)
ChemPlusChem     Hybrid Journal   (Followers: 2)
ChemTexts     Hybrid Journal  
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 11)
Chromatographia     Hybrid Journal   (Followers: 23)
Chromatography     Open Access   (Followers: 2)
Chromatography Research International     Open Access   (Followers: 6)
Cogent Chemistry     Open Access   (Followers: 2)
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 11)
Colloids and Interfaces     Open Access  
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 6)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 4)
Combustion Science and Technology     Hybrid Journal   (Followers: 22)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Communications Chemistry     Open Access  
Composite Interfaces     Hybrid Journal   (Followers: 7)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 1)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 1)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 9)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 12)
Computational Chemistry     Open Access   (Followers: 2)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 10)
Coordination Chemistry Reviews     Full-text available via subscription   (Followers: 4)
Copernican Letters     Open Access   (Followers: 1)
Corrosion Series     Full-text available via subscription   (Followers: 7)
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 8)
Croatica Chemica Acta     Open Access  
Crystal Structure Theory and Applications     Open Access   (Followers: 4)
CrystEngComm     Full-text available via subscription   (Followers: 13)
Current Catalysis     Hybrid Journal   (Followers: 2)
Current Chromatography     Hybrid Journal  
Current Green Chemistry     Hybrid Journal   (Followers: 1)
Current Metabolomics     Hybrid Journal   (Followers: 5)
Current Microwave Chemistry     Hybrid Journal  
Current Opinion in Colloid & Interface Science     Hybrid Journal   (Followers: 9)
Current Opinion in Molecular Therapeutics     Full-text available via subscription   (Followers: 14)
Current Research in Chemistry     Open Access   (Followers: 9)
Current Science     Open Access   (Followers: 73)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Dalton Transactions     Full-text available via subscription   (Followers: 23)
Detection     Open Access   (Followers: 4)
Developments in Geochemistry     Full-text available via subscription   (Followers: 2)

        1 2 3 4 | Last

Journal Cover
BioChip Journal
Journal Prestige (SJR): 0.412
Citation Impact (citeScore): 1
Number of Followers: 0  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1976-0280 - ISSN (Online) 2092-7843
Published by Springer-Verlag Homepage  [2352 journals]
  • Paper-Based Biochip Assays and Recent Developments: A Review
    • Authors: Moonseong Park; Byoung-Hoon Kang; Ki-Hun Jeong
      Pages: 1 - 10
      Abstract: Biomolecules in human body serve as biomarker for diagnosis of diseases, and paper-based biochips have become of much interest for development of biomolecule sensing. Cellulose micro-/nanofiber matrices consisting of paper allow not only capillary-driven flow without external pumping owing to abundant micro-/nanopores but also three-dimensional (3D) hierarchical micro-/nanostructures as 3D templates for microfluidic bioassays. Besides, colloidal and thermal evaporated metal nanoparticles on cellulose fibers offer huge opportunities in nanoplasmonic biosensing. Here, we review paper-based biochips including microfluidic paper-based assays and nanoplasmonic biosensors and further discuss micro-/nanofabrication of paper-based biochips and their applications in biomolecule detection.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2101-3
      Issue No: Vol. 12, No. 1 (2018)
       
  • Label-free Microarray-based Binding Affinity Constant Measurement with
           Modified Fluidic Arrangement
    • Authors: Junwei Hu; Ru Chen; Chenggang Zhu; Bilin Ge; Xiangdong Zhu; Lan Mi; Jiong Ma; Caiqin Han; Hao Chen; Yiyan Fei
      Pages: 11 - 17
      Abstract: The oblique-incidence reflectivity difference (OI-RD) scanning microscopy has the capability of simultaneously measuring binding curves of a protein probe with tens of thousands molecular targets in a microarray and yielding reaction rate constants. However, the quality of reaction rate constants is influenced by the fluidic system. To improve the quality of reaction rate constant measurement over the entire microarray, we demonstrate a fluidic chamber that allows the fluid to flow from the bottom to the top uniformly across the microarray and thus provides more uniform and accurate measurements of reaction rate constants with simplified fluidic design.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2102-2
      Issue No: Vol. 12, No. 1 (2018)
       
  • DAQ based Impedance Measurement System for Low Cost and Portable
           Electrical Cell-Substrate Impedance Sensing
    • Authors: Il-Hwan Park; Yeonhee Hong; Hee-Sook Jun; Eou-Sik Cho; Sungbo Cho
      Pages: 18 - 24
      Abstract: Electric cell-substrate impedance sensing (ECIS), a technique for label-free and real-time detection of cells, is emerging as an alternative or assistive method to traditional biochemical assays for diagnostic and pharmaceutical applications. In this work, we developed a DAQ based impedance measurement system for low cost and portable ECIS applications. The alternating input signal for ECIS was generated from the digital-to-analog converter (DAC) channel of the DAQ board and applied through the current limited resistor to the cells on the electrode. The responding signal was recorded by the analog-to-digital converter (ADC) channel of the DAQ board, and its amplitude and phase were analyzed by software lock-in amplifier. The feasibility of the developed ECIS system was evaluated by impedance measurement and DAPI analysis of C2C12 cell growth on the indium tin oxide microelectrode, and by impedimetric and MTT cell viability assay of HEK293 cells exposed to H2O2.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2103-1
      Issue No: Vol. 12, No. 1 (2018)
       
  • Bisphenol A Induces a Distinct Transcriptome Profile in the Male Fish of
           the Marine Medaka Oryzias javanicus
    • Authors: Bo-Mi Kim; Ye Jin Jo; Nayun Lee; Nayoung Lee; Seonock Woo; Jae-Sung Rhee; Seungshic Yum
      Pages: 25 - 37
      Abstract: While environmental concentration of bisphenol A (BPA) is ubiquitously detected in effluent and surface of freshwater, BPA has also been measured in coastal and marine ecosystems. To understand the effect of waterborne BPA on the transcriptome over time, male fish of the marine medaka Oryzias javanicus were exposed to 76 μg/L of BPA for 72 h. Time-course microarray study identified differentially expressed transcripts upon BPA exposure in the liver tissues in a time-dependent manner. The primary effect of BPA treatment appears to be significantly upregulated mRNA expression of lipid metabolism (e.g. fatty acid process, lipid/lipoprotein biosynthesis and transport, cholesterol regulation), while genes involved in diverse biochemical and physiological processes were downregulated. In particular, vertebrate immunity relevant genes showed strong downregulated patterns across time-courses. This study provides preliminary insights into the transcriptional response of marine medaka male fish to waterborne BPA exposure and suggests a basis for uncovering the mode of action particularly with respect to endocrine disrupting chemicals (EDCs) such as BPA.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2104-0
      Issue No: Vol. 12, No. 1 (2018)
       
  • Enhancement of Capturing Efficacy for Circulating Tumor Cells by
           Centrifugation
    • Authors: Doyeon Bang; Taeksu Lee; Joohyung Park; Gyudo Lee; Seungjoo Haam; Jinsung Park
      Pages: 38 - 45
      Abstract: Circulating tumor cells (CTCs), which are thought to be the main candidate for metastasis, are gaining importance owing to their potential impact on human health and public welfare. For capturing CTCs, antigen-antibody interaction has been used in which specific antigens expressed on cell surface of CTCs can be bound to antibodies immobilized on substrates. Conventional detection methods for CTCs have often suffered not only from relatively low antigen-antibody coupling efficiency but also from cumbersome fabrication processes of micro/nano-structures for CTCs capture. Herein, we report a facile, robust antibody-based CTCs detection technique using centrifugal force, which can enhance the capturing efficacy of CTCs. We validate chemical functionalization process of antibodies on a silica substrate by using atomic force microscopy. Furthermore, it turned out that the centrifugal force from a benchtop centrifuge is enough to produce a ~2.3-fold increase in capture yield of CTCs through enhancement of binding avidity between CTCs and the antibodies. Our result points out the great potential of our method for practical application in CTCs diagnostics and opens a new avenue for biological and chemical sensing.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2105-z
      Issue No: Vol. 12, No. 1 (2018)
       
  • Rapid Differentiation of Seven Species of Anguilla Using PNA
           Clamping-based Asymmetric PCR with Fluorescence Melting Curve Analysis
    • Authors: Eun Soo Noh; Hyun Sook Kang; Eun Mi Kim; Jae Koo Noh; Jung Youn Park; Tae-Jin Choi; Jung-Ha Kang
      Pages: 46 - 51
      Abstract: Differentiation of the genus Anguilla is important for phylogenetic studies, ecological monitoring, and food forensics. Although various methods have been developed to identify these species, current methods are time-consuming and limit the number of species that can be detected. The aim of this study was to develop a rapid and convenient method to identify the genus Anguilla. To differentiate among seven commercially important species of Anguilla (A. japonica, A. anguilla, A. rostrata, A. celebensis, A. pacifica, A. marmorata, and A. bicolor pacifica), fluorescence melting curve analysis (FMCA) with peptide nucleic acid (PNA) probes were used. One primer set was designed to amplify the mitochondrial cytochrome b gene, and seven PNA probes were designed to identify each species from the cyt b gene. The PNA probes were labeled with four different fluorescent reporter dyes (FAM, HEX, Texas Red, Cy5) for multiplex analysis using two tubes. Based on FMCA, each PNA probe showed the highest Tm value when it was 100% complementary to the target sequence. In conclusion, the PNA-based FMCA method is suitable for differentiating among seven species of Anguilla and should be applicable in various fields, such as global marketing and scientific research.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2106-y
      Issue No: Vol. 12, No. 1 (2018)
       
  • A Rapid Method for Estimation of Cariogenic Bacteria Based on a Stationary
           Liquid Phase Lab-on-a-chip
    • Authors: Hee-Jung Kim; Suk-Jung Choi
      Pages: 52 - 58
      Abstract: Salivary levels of Streptococcus mutans and Lactobacillus sp. are important risk factors for dental caries. Application of current test methods is limited because of the requirement for a time-consuming incubation step or expensive laboratory instruments. In this study, we developed a rapid method for determination of the representative cariogenic bacteria, S. mutans and Lactobacillus plantarum, based on a stationary liquid phase lab-on-a-chip (SLP LOC). The method utilized two types of particles, i.e., magnetic capture particles (CPs) for capture of target bacteria and nonmagnetic labeling particles (LPs) for labeling of the captured bacteria with horseradish peroxidase (HRP). Complexes of CP-bacteria-LP were formed in a sample chamber of the LOC and transported to a detection chamber containing the chromogenic substrate of HRP by moving a magnet under the LOC. The method is simple and enabled detection of the two bacteria in a range required for caries risk diagnosis within 40 min without the use of any complicated instruments. Accordingly, this method may have applications in the rapid diagnosis of dental caries risk.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2107-x
      Issue No: Vol. 12, No. 1 (2018)
       
  • Performance Characterization of Two-Dimensional Paper Chromatography-based
           Biosensors for Biodefense, Exemplified by Detection of Bacillus anthracis
           Spores
    • Authors: Seung-Mok Han; Young-Wan Kim; Young-Kee Kim; Jeong-Hoon Chun; Hee-Bok Oh; Se-Hwan Paek
      Pages: 59 - 68
      Abstract: Bacillus anthracis (B. anthracis), the causative agent of anthrax disease, is a Gram-positive spore-forming bacterium which can be used as a threatening bioterrorism agent. We developed enzyme-linked immunosorbent assay (ELISA)-on-a-chip biosensors for rapid, sensitive analysis of B. anthracis spores based on two-dimensional, cross-flow chromatography. In order to establish optimal assay conditions, a polyclonal antibody and four monoclonal antibodies against B. anthracis were raised and examined to characterize their analytical sensitivity as well as specificity. The biosensor results showed that a monoclonal antibody pair not only offered a relatively low detection limit for B. anthracis compared to other antibody combinations, but also displayed no cross-reactivity with other microorganisms belonging to the Bacillus genus. For detection of ELISA enzyme signal (e.g., horseradish peroxidase), chemiluminescent detection in combination with cooled charge-coupled device enhanced the sensor performance in terms of assay time, compared to that achieved by colorimetry. Under optimal conditions, the biosensor was able to detect a minimum threshold of 5×103 and 5×102 spores/mL for two different B. anthracis strains, NCCP 12860 (Sterne) and NCCP 10666 (Haman #1), respectively. Furthermore, the chemiluminometric sensor was minimally affected by the presence of potential interferents in samples such as baby powder, skim milk, and sucrose, indicating its potential utility for the analysis of bioterrorism agents directly in the field.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2108-9
      Issue No: Vol. 12, No. 1 (2018)
       
  • Development of a Sol-gel-assisted Reverse-phase Microarray Platform for
           Simple and Rapid Detection of Prostate-specific Antigen from Serum
    • Authors: SangWook Lee; Thomas Laurell; Ok Chan Jeong; Soyoun Kim
      Pages: 69 - 74
      Abstract: A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2109-8
      Issue No: Vol. 12, No. 1 (2018)
       
  • Identification of Possibility of Glycyrrhiza uralensis as an Allergen by
           Protein Analysis
    • Authors: Eun-Jin An; Kang-Hoon Kim; In-Seung Lee; Ji Young Park; Yumi Kim; Woo Sang Jung; Daeho Kwon; Hyeung-Jin Jang
      Pages: 75 - 82
      Abstract: Various secondary metabolites derived from plants are useful for humans to preserve their health. For this reason, medical herbs have been consumed universally without deep research about the dangerousness of these substance. However, they can produce unexpected adverse reactions in humans. Among these side effects, allergic reactions involving human immunoglobulin E can occur due to antimicrobial or antibacterial substances in plants. Glycyrrhiza uralensis has been prescribed for the treatment of numerous diseases given its multiple types of pharmaceutical efficacy, including its antimicrobial and antibacterial activities. Therefore, we researched whether Glycyrrhiza uralensis, which has antimicrobial ability, can cause allergic reaction in humans. To test the hypothesis devised here, we conducted proteomic level experiments. We utilized SDS-PAGE using a protein extract of Glycyrrhiza uralensis, with Coomassie blue staining to confirm the protein pattern and western blotting to identify Human IgE binding proteins in Glycyrrhiza uralensis. Among the many candidate sera, these methods detected three positive sera. Moreover, an identified protein section with reactivity associated with Human IgE was analyzed by LC-MS/ MS. Our experimental data provide a basis for testing the possibility of Glycyrrhiza uralensis as an allergen by identifying the reactivity between human Immunoglobulin E and proteins of Glycyrrhiza uralensis.
      PubDate: 2018-03-01
      DOI: 10.1007/s13206-017-2110-2
      Issue No: Vol. 12, No. 1 (2018)
       
  • Immunoreaction-based Microfluidic Diagnostic Device for the Detection of
           Prostate-Specific Antigen
    • Authors: Hyung Jin Kim; Byeong Hee Kim; Young Ho Seo
      Abstract: This paper addresses an immunoreaction period-independent microfluidic diagnostic method for the detection of prostate-specific antigen. Immunoreaction-based biosensors generally use sandwich binding of a capture antibody, followed by an antigen, and then a detection antibody with a fluorescent label. Fluorescent intensity is proportional not only to the antigen density in a sample but also to the immunoreaction period. Generally, immunoreaction-based sensors are dependent on the immunoreaction period because the amount of antigen bound to fluorescent labels increases as the immunoreaction period increases. A wash-out step is suggested to eliminate dependence on a specific reaction period. Further, the optimal time to start the wash-out step was investigated. For the conventional detection process, fluorescent intensity increases constantly as the immunoreaction period increases, even though the antigen concentration in the sample has not changed. After applying the wash-out process, however, the fluorescent intensity was maintained over a 15-min period. Consequently, even though a user may miss the optimal reading time for the assay, an accurate result can be reported because the wash-out step enables microfluidic diagnostic devices to maintain a constant fluorescent intensity.
      PubDate: 2018-05-23
      DOI: 10.1007/s13206-017-2208-6
       
  • Genome-wide Identification and Expression Analyses of RPP13-like Genes in
           Barley
    • Authors: Jinjin Cheng; Hui Fan; Lin Li; Boyao Hu; Hongyun Liu; Zheng Liu
      Abstract: Plants have evolved a series of mechanisms to resist pathogens infection. The nucleotide-binding site and leucine-rich repeat (NBS-LRR) family contains the largest number of plant disease resistance genes in plants. Recognition of Peronospora Parasitica 13-like (RPP13-like) genes belong to this superfamily and play important roles in the resistance of various plant diseases including the downy mildew caused by Peronospora parasitica. In this study, 21 RPP13-like genes were identified in barley via bioinformatics. These genes all contained CC, NB-ARC and LRR domains. The physical and chemical properties, chromosome locations, gene structures, protein motifs, 3D protein structures, and microarray based expression dynamics of these genes, as well as their phylogenetic relationship with other plant species were analyzed. Non-expression of MLOC_19262.1 was detected without pathogen infection. When barley was inoculated with the powdery mildew pathogenic fungus, the expression of MLOC_ 19262.1 reached a very high level, suggesting that this gene is an important and promising candidate resistance gene for further study. The two RPP13-like genes, MLOC_57007.2 and MLOC_5059.1 may be involved in barley regular or abiotic stress induced physiological metabolism in specific tissues or at specific developmental stages; furthermore, these functions may be associated with specific domains. These findings provided evidence for the functional diversity of plant pathogen resistance genes and will be helpful for the future characterization of the PRR13-like gene subfamily.
      PubDate: 2018-05-18
      DOI: 10.1007/s13206-017-2203-y
       
  • Dual Applicability of Polyaniline Coated Gold Nanorods: A Study of
           Antibacterial and Redox Activity
    • Authors: Xiaohong Liang; Saravanan Govindaraju; Kyusik Yun
      Abstract: Non-agglomerated, dual-purpose monodispersed gold nanorods (GNRs) were synthesized by using a seed-mediated method, and subsequent in situ chemical polymerization yielded polyaniline-coated gold nanorods (PANI-GNRs). The synthesized GNRs and PANI-GNRs were characterized by electron microscopic analyses, selected-area electron diffraction patterns, and element analyses. The prepared nanomaterials had an average length of 40±0.4 nm and a diameter of 15±0.2 nm. Furthermore, the PANI coating around the GNRs had a thickness of ~21 nm. These nanomaterials were also characterized by ultraviolet- visible and Fourier transform-infrared spectroscopies. While the absorption peaks of GNRs were observed at 520 and 675 nm, those of PANI-GNRs showed absorption maxima at 325 and 665 nm. The combination of 0.91 vol% of aniline with 5 mL of GNRs provided better electrochemical properties at 0.52 V and 1.30 V. The minimum inhibitory concentrations of laser-irradiated PANI-GNRs, PANI-GNRs, and laser-irradiated GNRs against Escherichia coli and Staphylococcus aureus were determined by using the micro-dilution method and compared to those of kanamycin (standard drug), revealing the significant bactericidal activity of laser-irradiated PANI-GNRs. This activity of the PANI-GNRs was also supported by the results of fluorescence and atomic force microscopy imaging. Thus, the synthesized nanomaterials can be potentially utilized for waste water treatment and biomedical/ pharmaceutical applications.
      PubDate: 2018-05-16
      DOI: 10.1007/s13206-017-2206-8
       
  • Electrostatically Self-assembled Quinazoline-based Anticancer Drugs on
           Negatively-charged Nanodiamonds for Overcoming the Chemoresistances in
           Lung Cancer Cells
    • Authors: Anh Thu Ngoc Lam; Jin-Ha Yoon; Nguyen Hoang Ly; Sang-Woo Joo
      Abstract: The nanodiamond (ND) conjugates of gefitinib (GF) and erlotinib (EL) were assembled for in vitro lung cancer treatments. The carboxylate infrared bands along with the negative surface charge of -28.3 (±2.1) mV were found efficient to conjugate the nitrogen-containing quinazoline ring drugs, due to the electrostatic interactions, resulting from the surface changes to -12.0 (±1.2) mV and -9.1 (±1.2) mV after adsorption of GF and EL on NDs, respectively. The physicochemical properties of NDs were characterized by transmission electron microcopy, X-ray diffraction, X-ray photoelectron, infrared and Raman spectroscopic tools. The size distributions of NDs after the self-assembly of GF and EL could be checked by dynamic light scattering measurements. The uptake of NDs in cancer cells was estimated by fluorescence microscopy. Cell viability appeared to decrease by 30-50% at 50 and 100 nM of GF and EL, respectively, after the treatment of PEG-assembled NDs compared to the cases using free drugs. Our ND conjugates may be potentially useful for overcoming the chemoresistances in lung cancer cells.
      PubDate: 2018-05-16
      DOI: 10.1007/s13206-017-2209-5
       
  • Dielectrophoresis Microfluidic Enrichment Platform with Built-In
           Capacitive Sensor for Rare Tumor Cell Detection
    • Authors: Loc Quang Do; Ha Tran Thi Thuy; Tung Thanh Bui; Van Thanh Dau; Ngoc-Viet Nguyen; Trinh Chu Duc; Chun-Ping Jen
      Abstract: The manipulation and detection of rare cells are important for many applications in early disease diagnosis and medicine. This study presents a dielectrophoresis (DEP) microfluidic enrichment platform combined with a built-in capacitive sensor for circulating tumor cell detection. The microchip is composed of a lollipop-shaped gold microelectrode structure under a polydimethylsiloxane chamber. A prototype of the device was fabricated using standard micromachining technology. With the proposed device, target cells (in this study, A549 non-small human lung carcinoma and S-180 sarcoma cell lines) are firstly guided toward the center of the working chamber via DEP forces. Then, the target cells are captured by an electrode immobilized by anti-EGFR, which has high affinity toward the target cells. After the cell concentration process, the differential capacitance is read to detect the presence of the target cells. Numerical simulations and measurement experiments were performed to demonstrate the high sensitivity of differential capacitive sensing. The obtained results show high sensitivity for S-180 cell detection (3 mV/cell). The proposed platform is suitable for rapid cancer diagnoses and other metabolic disease applications.
      PubDate: 2018-04-27
      DOI: 10.1007/s13206-017-2204-x
       
  • A Microfluidic Hydrogel Chip with Orthogonal Dual Gradients of Matrix
           Stiffness and Oxygen for Cytotoxicity Test
    • Authors: Weixing Wang; Lili Li; Mingyu Ding; Guoan Luo; Qionglin Liang
      Abstract: The oxygen tensions and matrix stiffness play important roles in regulating cell response to cytotoxic drugs. In recent years, single spatiotemporal oxygen or matrix stiffness gradient has been established by various technologies for cell studies. However, the synergistic effects of the two factors on tumor cells remained elusive. In this study, we created a highly integrated and easy-to-operate microfluidic device. It was capable of generating more continuous, linear, stable and diffusive hydrogel stiffness gradient over a well-defined oxygen gradient. Sodium hydroxide and pyrogallol were used to scavenge oxygen and generate oxygen gradient, skillfully avoiding the utilization of bulky pressurized gas cylinders and sophisticated flow control by conventional methods. Utilizing the newly developed microfluidic device, we successfully performed drug test with a hypoxia sensitive anti-cancer drug, triapazamine (TPZ), on A549 cells under perpendicular construction of oxygen and spatially hydrogel stiffness gradients. The cell experiment results demonstrated the matrix stiffness-dependent cell drug resistance and hypoxia-induced cytotoxicity of TPZ. In summary, the developed microfluidic device exhibits high potential in the study of matrix stiffness-dependent cell responses and oxygen-sensitive drug cytotoxicity. Furthermore, the design principle can be functionally extended to other dual factors system and will facilitate the study of cell response in complex physiological and pathological conditions.
      PubDate: 2018-04-27
      DOI: 10.1007/s13206-017-2202-z
       
  • Droplet-based digital PCR system for detection of single-cell level of
           foodborne pathogens
    • Authors: Minjeong Jang; Soon Woo Jeong; Nam Ho Bae; Younseong Song; Tae Jae Lee; Moon Keun Lee; Seok Jae Lee; Kyoung G. Lee
      Abstract: Recently, foodborne pathogen is a common and distressing disease around world to cause a threat to life and economic damages and it is urgent to develop a tools to diagnosis of such pathogens in the early stage to prevent potential outbreak. Although conventional cell extraction and recovery of DNA from pathogen and PCR method have been widely used, the methods require complex steps, experts, and expensive chemicals and instruments to improve the PCR performance. Herein, we report a droplet-based polymerase chain reaction (ddPCR) system which allows identifying single-cell level of foodborne pathogens. E. coli O157:H7 and Salmonella cells were selected as model bacterial foodborne pathogens. The ddPCR system could be a useful platform for the quantitative detection of foodborne pathogens without any pretreatment process.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1410-x
       
  • Direct buffer composition of blood pre-process for nucleic acid based
           diagnostics
    • Authors: YeJi Kim; Won-Nyoung Lee; Hyun Jin Yoo; Changyoon Baek; Junhong Min
      Abstract: Recently, a variety of methods, so called “direct buffer”, have been developed to utilize nucleic acid in the blood for the measurement of infectious bacteria and virus without any equipment in the field. In here, we first investigated the individual and combinatory effects of candidate chemicals which might be composed of the direct buffer on the PCR inhibition reduction of main compositions in whole blood. The long and short PEGs, Na2SO4 and GuSCN were selected as representative kosmotropic and chaotropic salts, respectively. MgCl2 were chosen as divalent cation source and NaOH was used to control blood pH. The effect of common non-ionic biological detergent was tested with Triton X-100 and SDS (Sodium Dodecyl sulfate) was chosen as anionic detergent. These results could provide a foundation for the development of sample preparation solution in nucleic acid based diagnostic field. As a result, the direct buffer developed in this study was able to detect viruses with a concentration of 102 pfu/100 μL of whole blood by a very simple method.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1401-y
       
  • Colorimetric Schirmer strip for tear glucose detection
    • Authors: Byoung-Hoon Kang; Moonseong Park; Ki-Hun Jeong
      Abstract: Human tears have attracted attention with easy-accessible indicators of healthcare monitoring. Recently self-monitoring of tear glucose in place of blood glucose has been developed with microfluidic paper-based analysis for a non-invasive diagnosis of diabetes. However, most previous works still have many limitations in unstable sample collection, low sensitivity, and insufficient sample volume. Here, we report a colorimetric Schirmer strip with preconcentration for tear glucose detection. A wax barrier on a paper strip enables tear collection of a fixed quantity as well as biocompatible incorporation of tear collection and detection by preventing flow reversal of reagents. Additional solvent was injected on a sampling site after tear collection, which accumulates the dispersed glucose into active site by capillary-driven flow for signal amplification. Glucose concentration was quantitatively detected at 0.1-2 mM by using a simple colorimetric method without any further image processing. The color changes in glucose concentration were clearly distinguishable between normal and diabetic patient-level. The colorimetric paper strip allows straightforward sensing platform for noninvasive diagnosis of diabetes and direct application of paper-based technology to the human body fluids.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1405-7
       
  • Microfabricated cell culture system for the live cell observation of the
           multilayered proliferation of undifferentiated HT-29 cells
    • Authors: Young Lee; Je-Kyun Park
      Abstract: In this paper, we describe a microfabricated cell culture system (MCCS) for the microscopic live observation of cellular behavior in a reconstituted tissue-like microenvironment. The MCCS was used as a novel tool for tracking the proliferation of undifferentiated HT-29 cells. A wall structure of gelled extracellular matrix (ECM) protein was constructed inside the MCCS to establish a tissue-like microenvironment of the intestinal tissue. The ECM wall consisting of type I collagen retained the structural integrity during the gelation process and provided the adhesion surface for the HT-29 cells. We carried out the microscopic live observation of the proliferation of HT-29 cells seeded with various concentrations. The multilayered growth of HT-29 cells in the undifferentiated state was monitored without cell fixation, which was a necessary process in a conventional in vitro cell culture. The live tracking of cell culture also recorded the contraction of cell culture morphology during the initial period of cultivation. This approach is helpful for the establishment of an in vitro model for the live observation study of intestinal epithelial differentiation in an in vivo-like microenvironment.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1407-5
       
 
 
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