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  Subjects -> CHEMISTRY (Total: 846 journals)
    - ANALYTICAL CHEMISTRY (50 journals)
    - CHEMISTRY (597 journals)
    - CRYSTALLOGRAPHY (22 journals)
    - ELECTROCHEMISTRY (25 journals)
    - INORGANIC CHEMISTRY (41 journals)
    - ORGANIC CHEMISTRY (45 journals)
    - PHYSICAL CHEMISTRY (66 journals)

CHEMISTRY (597 journals)                  1 2 3 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 7)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 26)
ACS Catalysis     Full-text available via subscription   (Followers: 31)
ACS Chemical Neuroscience     Full-text available via subscription   (Followers: 17)
ACS Combinatorial Science     Full-text available via subscription   (Followers: 23)
ACS Macro Letters     Full-text available via subscription   (Followers: 22)
ACS Medicinal Chemistry Letters     Full-text available via subscription   (Followers: 39)
ACS Nano     Full-text available via subscription   (Followers: 217)
ACS Photonics     Full-text available via subscription   (Followers: 10)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 20)
Acta Chemica Iasi     Open Access   (Followers: 2)
Acta Chimica Sinica     Full-text available via subscription  
Acta Chimica Slovaca     Open Access   (Followers: 1)
Acta Chromatographica     Full-text available via subscription   (Followers: 9)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 5)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 5)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 7)
Adsorption Science & Technology     Full-text available via subscription   (Followers: 5)
Advanced Functional Materials     Hybrid Journal   (Followers: 48)
Advanced Science Focus     Free   (Followers: 3)
Advances in Chemical Engineering and Science     Open Access   (Followers: 53)
Advances in Chemical Science     Open Access   (Followers: 12)
Advances in Chemistry     Open Access   (Followers: 12)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Enzyme Research     Open Access   (Followers: 10)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 14)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 8)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 18)
Advances in Nanoparticles     Open Access   (Followers: 12)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15)
Advances in Polymer Science     Hybrid Journal   (Followers: 40)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 18)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Science and Technology     Full-text available via subscription   (Followers: 10)
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 2)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 7)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 65)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 14)
American Journal of Chemistry     Open Access   (Followers: 25)
American Journal of Plant Physiology     Open Access   (Followers: 13)
American Mineralogist     Full-text available via subscription   (Followers: 12)
Analyst     Full-text available via subscription   (Followers: 38)
Angewandte Chemie     Hybrid Journal   (Followers: 153)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 203)
Annales UMCS, Chemia     Open Access   (Followers: 1)
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 1)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 3)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 7)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 14)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antiviral Chemistry and Chemotherapy     Hybrid Journal  
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 6)
Applied Spectroscopy     Full-text available via subscription   (Followers: 22)
Applied Surface Science     Hybrid Journal   (Followers: 26)
Arabian Journal of Chemistry     Open Access   (Followers: 6)
ARKIVOC     Open Access   (Followers: 2)
Asian Journal of Biochemistry     Open Access   (Followers: 1)
Atomization and Sprays     Full-text available via subscription   (Followers: 3)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 2)
Avances en Quimica     Open Access   (Followers: 1)
Biochemical Pharmacology     Hybrid Journal   (Followers: 9)
Biochemistry     Full-text available via subscription   (Followers: 277)
Biochemistry Insights     Open Access   (Followers: 5)
Biochemistry Research International     Open Access   (Followers: 6)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 9)
Bioinspired Materials     Open Access   (Followers: 3)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access  
Biomacromolecules     Full-text available via subscription   (Followers: 18)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 10)
Biomedical Chromatography     Hybrid Journal   (Followers: 6)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 4)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 107)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 99)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 18)
Biosensors     Open Access   (Followers: 2)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 2)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 24)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 2)
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 2)
Canadian Journal of Chemistry     Full-text available via subscription   (Followers: 10)
Canadian Mineralogist     Full-text available via subscription   (Followers: 3)
Carbohydrate Research     Hybrid Journal   (Followers: 26)
Carbon     Hybrid Journal   (Followers: 67)
Catalysis for Sustainable Energy     Open Access   (Followers: 6)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 8)
Catalysis Science and Technology     Free   (Followers: 6)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 7)
Cellulose     Hybrid Journal   (Followers: 7)
Cereal Chemistry     Full-text available via subscription   (Followers: 4)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 1)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 12)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 69)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 23)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Full-text available via subscription   (Followers: 19)
Chemical Reviews     Full-text available via subscription   (Followers: 165)
Chemical Science     Open Access   (Followers: 21)
Chemical Technology     Open Access   (Followers: 15)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 4)
Chemical Week     Full-text available via subscription   (Followers: 7)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 55)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 25)
ChemInform     Hybrid Journal   (Followers: 7)
Chemistry & Biodiversity     Hybrid Journal   (Followers: 6)
Chemistry & Biology     Full-text available via subscription   (Followers: 30)
Chemistry & Industry     Hybrid Journal   (Followers: 5)
Chemistry - A European Journal     Hybrid Journal   (Followers: 136)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 15)
Chemistry and Materials Research     Open Access   (Followers: 17)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Hybrid Journal   (Followers: 2)
Chemistry Letters     Full-text available via subscription   (Followers: 43)
Chemistry of Materials     Full-text available via subscription   (Followers: 189)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 9)
Chemistry-Didactics-Ecology-Metrology     Open Access  
ChemistryOpen     Open Access   (Followers: 2)
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 2)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 15)
Chemosensors     Open Access  
ChemPhysChem     Hybrid Journal   (Followers: 8)
ChemPlusChem     Hybrid Journal   (Followers: 2)
ChemTexts     Hybrid Journal  
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 10)
Chromatographia     Hybrid Journal   (Followers: 23)
Chromatography Research International     Open Access   (Followers: 7)
Clay Minerals     Full-text available via subscription   (Followers: 9)
Cogent Chemistry     Open Access  
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 10)
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 8)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 3)
Combustion Science and Technology     Hybrid Journal   (Followers: 18)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Composite Interfaces     Hybrid Journal   (Followers: 6)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 2)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 1)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 9)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 12)
Computational Chemistry     Open Access   (Followers: 2)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 9)
Coordination Chemistry Reviews     Full-text available via subscription   (Followers: 2)
Copernican Letters     Open Access  
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 5)
Crystal Structure Theory and Applications     Open Access   (Followers: 3)
CrystEngComm     Full-text available via subscription   (Followers: 10)
Current Catalysis     Hybrid Journal   (Followers: 2)
Current Metabolomics     Hybrid Journal   (Followers: 4)
Current Opinion in Colloid & Interface Science     Hybrid Journal   (Followers: 9)
Current Research in Chemistry     Open Access   (Followers: 8)
Current Science     Open Access   (Followers: 48)
Dalton Transactions     Full-text available via subscription   (Followers: 18)
Detection     Open Access   (Followers: 2)
Developments in Geochemistry     Full-text available via subscription   (Followers: 2)
Diamond and Related Materials     Hybrid Journal   (Followers: 11)
Dislocations in Solids     Full-text available via subscription  
Doklady Chemistry     Hybrid Journal  
Drying Technology: An International Journal     Hybrid Journal   (Followers: 3)
Eclética Química     Open Access   (Followers: 1)
Ecological Chemistry and Engineering S     Open Access   (Followers: 4)
Ecotoxicology and Environmental Contamination     Open Access  
Educación Química     Open Access   (Followers: 1)
Education for Chemical Engineers     Hybrid Journal   (Followers: 5)
EJNMMI Radiopharmacy and Chemistry     Open Access  
Elements     Full-text available via subscription   (Followers: 2)
Environmental Chemistry     Hybrid Journal   (Followers: 8)
Environmental Chemistry Letters     Hybrid Journal   (Followers: 4)
Environmental Science & Technology Letters     Full-text available via subscription   (Followers: 5)
Environmental Science : Nano     Partially Free   (Followers: 1)
Environmental Toxicology & Chemistry     Hybrid Journal   (Followers: 19)

        1 2 3 | Last

Journal Cover BioChip Journal
  [SJR: 0.368]   [H-I: 12]   [0 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1976-0280 - ISSN (Online) 2092-7843
   Published by Springer-Verlag Homepage  [2329 journals]
  • Disrupted pathways associated with neonatal sepsis: Combination of
           protein-protein interactions and pathway data
    • Authors: Xin Qiao; Silian Zhu; Shujiao Zhang; Hongmei Dong
      Pages: 1 - 7
      Abstract: Abstract To identify disrupted pathways associated with neonatal sepsis, we performed a research based on the combination of protein-protein interactions (PPIs) and pathway data. Firstly, a total of 23,292 genes, 787,896 PPIs and 1,675 human pathways were obtained, respectively. Then, under the threshold value of false discovery rate (FDR)<0.05 and a delta cut-off value >4.36, a total of 986 differentially expressed genes (DEGs) were identified. In the following, by degree centrality for the objective PPI network, 20 hub genes were obtained. Finally, pathway enrichment analysis and randomization tests indicated that pathways of gene expression, immune system and innate immune system were with remarkable significance in neonatal sepsis. Therefore, in the present study, we presented a novel pathway method, and we successfully identified several pathways in neonatal sepsis, which might be underlying indicators in the detection and treatment of neonatal sepsis.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1101-z
      Issue No: Vol. 11, No. 1 (2017)
       
  • Rapid visual identification of bovine meat by loop mediated isothermal
           amplification combined with immunochromatographic strip
    • Authors: Yong-Jin Li; Jin-Yu Fan
      Pages: 8 - 13
      Abstract: Abstract Considering the factor of culture, religion, health and commerce, the development of a rapid, convenient and sensitive method is important for the detection of the presence of meat species in raw or processed foods. In this study, we employed loop mediated isothermal amplification (LAMP) for species-specific target gene amplification, and the amplicons were analyzed on the immunochromatographic strip (ICS) for the rapid visual identification of meat species. Bovine meat was selected as an example to confirm the performance of such assay. The LAMP based ICS method show good specificity, repeatability and has a limit of detection of 0.1% for beef in meat mixture. The whole detection process could be completed within 50 min. Our method is more convenient and rapid compared to classical LAMP. Moreover, it could be easily adapted to identify other types of meats, and it may be useful for food administration laboratories to carry out meat species identification in raw and processed foods.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1102-y
      Issue No: Vol. 11, No. 1 (2017)
       
  • Cholecystokinin as a potent diagnostic marker for gastric cancer
    • Authors: Hafeza Akter; Young Sook Yoo; Won Sang Park; Min-Jung Kang
      Pages: 14 - 20
      Abstract: Abstract There is no early diagnosis marker for gastric cancer even though the gastric cancer is the second cause of deaths in East Asia. The aim of the current research is to develop a new diagnostic marker of gastric cancer. Commercially available ELISA kits were used to evaluate the peptide levels in plasma collected from Korean healthy and gastric cancer patients. About 1.5 fold decreased plasma cholecystokinin levels were measured in gastric cancer patients (n=75) than in healthy controls (n=36). Plasma cholecystokinin has no age, sex, and disease-dependent variation in control and patients sample. Receiver-operating characteristic curve analysis assessing the performance of cholecystokinin levels as the sensitivity of 94.7% and specificity of 100 % compared to healthy controls. These findings suggest plasma cholecystokinin as a useful biomarker for diagnosis of gastric cancer.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1103-x
      Issue No: Vol. 11, No. 1 (2017)
       
  • Assessment for quantification of biopharmaceutical protein using a
           microvolume spectrometer on microfluidic slides
    • Authors: Jaehee Byeon; Kui Hyun Kang; Hoe-Kyung Jung; Jung-Keun Suh
      Pages: 21 - 29
      Abstract: Abstract Spectrophotometric procedure is a traditional method for quantifying protein concentration in solution. The benefit of this method is simple and direct, not requiring standard materials, sample incubation, and exogenous chromophore addition. There are also some drawbacks, low detection limits and high amounts of protein needed. The recent advances in fiber optics and nano-technology enable to overcome those drawbacks by using microvolume sampling and extremely short pathlengths. In this study, the utility of the microvolume system with microfluidic carriers was assessed for concentration measurement of biopharmaceutical protein using absorbance at 280 nm and also compared to a traditional instrument. The results show a high correlation of estimation by Cohen’s d effect size and Pearson correlation coefficient between two different spectrometers. For UV spectral analysis, there are no statistically significant difference between those spectra from a microvolume spectrometer and a traditional one. For the extinction coefficient determination, two different spectrometers also give quite comparable and similar values. Thus a microvolume spectrometer can be used as an alternative over a traditional one to determine the concentration of biopharmaceutical protein. Moreover, the dynamic range for protein quantification by a microvolume spectrometer is over 10-fold higher than that of a traditional one.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1104-9
      Issue No: Vol. 11, No. 1 (2017)
       
  • Development of an improved stationary liquid-phase lab-on-a-chip for the
           field monitoring of paralytic shellfish toxins
    • Authors: Eunkyeong Yu; Suk-Jung Choi
      Pages: 30 - 38
      Abstract: Abstract A stationary liquid-phase lab-on-a-chip (SLP-LOC), in which magnetic particles are used as a solid phase, has been previously reported as a novel platform for immunoassay methods. The SLP-LOC had a simple structure with two chambers connected by a narrow channel. The assay was performed by transporting the particles from one chamber to the next while the liquid phases were in a stationary state. However, each chamber was frequently contaminated by other chamber materials because of the small volume of the channel. Consequently, in this work, we have developed a three-chamber SLP-LOC (3C-LOC) featuring an additional chamber containing a buffered washing solution positioned between the existing two chambers and connected by channels. The dimensions of the 3C-LOC were optimized to fit into a standard spectrophotometer cuvette holder, allowing the direct measurement of absorbance. The 3C-LOC exhibited improved washing efficiency, reliability, and sensitivity in an assay of standard saxitoxin samples. Furthermore, the method was used to effectively determine whether paralytic shellfish toxin (PST) levels in shellfish samples exceeded regulatory limits. The results of this study indicate that the 3C-LOC could provide a simple and rapid method to monitor PSTs in the field.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1105-8
      Issue No: Vol. 11, No. 1 (2017)
       
  • Assessment of endothelial cell growth behavior in thin film nitinol
    • Authors: Yanfei Chen; Mahdis Shayan; Woon-Hong Yeo; Youngjae Chun
      Pages: 39 - 45
      Abstract: Abstract Growing clinical needs for less invasive endovascular treatments necessitate the development of advanced biomaterials that exhibit low-profile and enhanced biocompatible properties. One of the endovascular devices is a stent graft, which contains a metallic backbone, covered with thin polymeric membranes such as Dacron® and expandable polytetrafluoroethylene (ePTFE). This device has been widely used for treating various vascular diseases and injuries. While the commercial materials including Dacron® and ePTFE have demonstrated a good feasibility, they were found to induce inflammatory vessel wall reactions with neointimal hyperplasia. Consequently, it causes re-narrowing of the lumen space and thrombogenic issues that ultimately lead the treatment failure. In this paper, we introduced a thin film nitinol (TFN) as an alternative graft material and evaluated the growth behavior of endothelial cells (EC) both qualitatively and quantitatively. As a proof-of-concept study, both untreated nonpatterned film (TFN) and surface treated TFN (S-TFN) materials were used. We compared the adhesion, growth, and proliferation of ECs on a solid (non-patterned) TFN with the two most widely-used commercial graft materials (Dacron® and ePTFE). The in vitro experimental results showed better adhesion and growth of ECs on TFN materials than either ePTFE or Dacron®. Specifically, S-TFN showed approximately twice number of ECs attached on the surface than any other materials tested in this study. In addition, in vivo swine study demonstrated that ECs had a relatively high proliferation on the micropatterrned S-TFN with ~50% surface coverage within two weeks. Both in vitro and in vivo study results of cell growth suggested that TFN materials could be a promising graft material for low-profile endovascular devices.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1106-7
      Issue No: Vol. 11, No. 1 (2017)
       
  • Glucose detection using 4-mercaptophenyl boronic acid-incorporated silver
           nanoparticles-embedded silica-coated graphene oxide as a SERS substrate
    • Authors: Xuan-Hung Pham; Seongbo Shim; Tae-Han Kim; Eunil Hahm; Hyung-Mo Kim; Won-Yeop Rho; Dae Hong Jeong; Yoon-Sik Lee; Bong-Hyun Jun
      Pages: 46 - 56
      Abstract: Abstract In this work, 4-mercaptophenyl boronic acid (4-MPBA) was self-assembled on the surface of silver nanoparticle-embedded silica-coated graphene oxide (GO@SiO2@Ag NPs@MPBA) to detect glucose by surface enhanced Raman scattering (SERS). The SERS intensity of 4-MPBA on the GO@SiO2@Ag NPs was 2.2-fold greater than that of GO@Ag NPs. Moreover, silica-coated GO exhibited lower background signals compared to GO. The SERS intensity of GO@SiO2@Ag NPs@MPBA peaked at 1 mM 4-MPBA. pH-dependent behavior of 4-MPBA on the GO@SiO2 @Ag NPs was investigated; the highest SERS signal intensity was detected at pH 3. The binding of glucose to 4-MPBA-incorporated GO@SiO2@Ag NPs increased the SERS signals at both 1,072 and 1,588 cm-1. The linear range was estimated from 2 to 20 mM glucose. These results provide insight into detection of glucose and the development of SERS-based biosensors using graphene oxide.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1107-6
      Issue No: Vol. 11, No. 1 (2017)
       
  • In situ PKA activity assay by selective detection of its catalytic subunit
           using antibody arrays
    • Authors: Danishmalik Rafiq Sayyed; Se-Hui Jung; Min-Soo Kim; Eun-Taek Han; Won Sun Park; Seok-Ho Hong; Young-Myeong Kim; Kwon-Soo Ha
      Pages: 57 - 66
      Abstract: Abstract Protein kinase A (PKA) plays a pivotal role in various biological processes and the pathogenesis of several diseases. However, systematic investigation of PKA functions in cells and tissues is limited due to the lack of a suitable high-throughput in situ PKA activity assay. Here, we present an array-based in situ PKA activity assay that employs selective detection of the catalytic form of PKA (cPKA; active and autophosphorylated) using antibody arrays. Antibody arrays were fabricated by applying anti-cPKA antibody onto welltype amine arrays. The limit of detection was 0.02 μg/ mL. We successfully applied this assay to determine changes in intracellular and extracellular PKA activities in human gastric adenocarcinoma (AGS) cells and human umbilical vein endothelial cells (HUVECs) treated with forskolin. Forskolin induced activation of intracellular PKA in a dose-dependent manner, and this PKA activation was inhibited by the potent PKA inhibitor H-89. Extracellular PKA activity was also elevated by forskolin in a dose-dependent manner in AGS cells, but this elevation was hardly detectable in HUVECs. Thus, our antibody array-based in situ PKA activity assay is suitable for investigating the regulation and functions of intracellular and extracellular PKA in cells and tissues and has a potential for use in cancer diagnosis.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1108-5
      Issue No: Vol. 11, No. 1 (2017)
       
  • Fabrication and characterization of PVCP human breast tissue-mimicking
           phantom for photoacoustic imaging
    • Authors: Eun-ju Jeong; Hyun-Woo Song; Yong-Jae Lee; Su Jun Park; Mi Jung Yim; Su Sung Lee; Bong Kyu Kim
      Pages: 67 - 75
      Abstract: Abstract Human tissue-mimicking phantoms are important for characterizing and optimizing ultrasound and photoacoustic imaging systems. We demonstrated the applicability of a polyvinyl chloride plastisol (PVCP) phantom to photoacoustic imaging studies. The acoustic properties of the fabricated PVCP phantom were measured to have a sound speed of 1370 m/s, attenuation coefficient of 0.71 dB/cm/MHz, and acoustic impedance of 1.39 Mrayl. Reduced scattering coefficients, absorption coefficient, and refractive index were obtained, as 1.31 mm-1, 0.001 mm-1, and 1.636 at 780 nm by optical measurements, respectively. Therefore, the PVCP phantom was found to be similar enough to human breast tissue in photoacoustic properties.
      PubDate: 2017-03-01
      DOI: 10.1007/s13206-016-1109-4
      Issue No: Vol. 11, No. 1 (2017)
       
  • PDMS microchannel surface modification with Teflon for algal lipid
           research
    • Authors: Jae Woo Park; Sangcheol Na; Myeongwoo Kang; Sang Jun Sim; Noo Li Jeon
      Abstract: Abstract This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs without background signal from absorbed dye molecules on PDMS microchannel. We confirmed that contact angle of Teflon-coated PDMS (116.4°) is higher than that of unmodified PDMS (106.1°) and thus more hydrophobic. Furthermore, Teflon-coated PDMS surface had ~80% of oxygen transfer rate compared to that of native PDMS and good transparency in all visible light regions. Based on these characteristics, we successfully validated the visualization and quantification of intracellular lipid droplets in microalgae C. reinhardtii using BODIPY. We believe that our new method will expand microfluidic applications on characterization of biological lipid with fluorescence probes and biochemical markers.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1302-0
       
  • Application of paper EWOD (electrowetting-on-dielectrics) chip: Protein
           tryptic digestion and its detection using MALDI-TOF mass spectrometry
    • Authors: Inae Jang; Hyojin Ko; Gwangro You; Hyunji Lee; Seunghwi Paek; Heedo Chae; Jae Hwan Lee; Sunkyung Choi; Oh-Sun Kwon; Kwanwoo Shin; Han Bin Oh
      Abstract: Abstract A paper-based open EWOD chip was used for protein tryptic digestion, and the resulting peptides were analyzed using MALDI-TOF mass spectrometry to identify the proteins. Although on-chip protein digestion was previously demonstrated on a glass-based EWOD platform, this is the first report to show that a paper-based EWOD can also be used for protein digestion and further analysis by mass spectrometry. A number of protein digestion processes, i.e., disulfide bond reduction, alkylation, buffering, and tryptic digestion, can be successfully conducted on the paper-based EWOD chip. Furthermore, a “Y”-shaped junction design was shown to be effective in successfully manipulating a droplet for protein digestion. A paper-based EWOD platform offers a cheap and versatile avenue for biological applications.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-016-1208-2
       
  • Affinity characteristic of terminal sequence in vancomycin resistant
           Enterococcus (VRE) membrane peptides on nanobiosensor chip using localized
           surface plasmon resonance
    • Authors: Kyungmin Lee; Yo-Han Kim; Hunsang Jung; Hyun Ho Lee
      Abstract: Abstract In this study, specific binding affinity between vancomycin (VAN) conjugated 40-50 nm gold nanoparticles (Au NPs) implemented on a sensor chip and pentapeptide mimicking pathogenic VAN resistant Enterococcus (VRE) wall membrane was employed as nanosensor format based on localized surface plasmon resonance (LSPR). Two pentapeptides, which were also anchored to 5 nm Au NPs, having two different terminal sequences of d-Ala-d-Ala and d-Ala-d-Lac respectively were compared in specific binding affinity toward the VAN conjugated nanosensor. Binding affinity difference identified by the LSPR characteristic in the nanosensor was shown in absorbance intensity and absorption wavelength shift between the two different terminal pentapeptides. The absorbance intensity clearly indicated that the pentapeptide having terminal d-Ala-d-Lac showed less affinity toward VAN conjugated nanosensor chip than that having terminal d-Ala-d-Ala. It indicates that the VRE itself has relatively weak binding strength towards the VAN, which may be used as a probe molecule for the VRE. Therefore, in order to design a VRE sensor using the VAN affinity, an indirect LSPR method can be devised instead of direct LSPR method.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-016-1206-4
       
  • Integrated miRNA and mRNA expression profiling in response to eriodictyol
           in human endothelial cells
    • Authors: Seung Eun Lee; Hye Rim Park; Hong Duck Yun; Jeong-Je Cho; Hyun-Jong Ahn; Cheung-Seog Park; Yong Seek Park
      Abstract: Abstract Eriodictyol, a flavonoid commonly found in citrus fruits, shows neurotrophic, antioxidant, anti- inflammatory, and anti-cancer effects under diverse pathophysiological conditions such as vascular diseases. In this study, we evaluated whether eriodictyol modulates miRNA and mRNA expression. Using microarray analysis, we examined miRNA and mRNA expression in human endothelial cells treated with 10 μM of eriodictyol for 24 h. Using numerous bioinformatic systems, we evaluated the signatures of the potential biological processes and signaling pathways. Our results provide insight into the underlying molecular mechanisms of action of eriodictyol and suggest that eriodictyol can be used for therapeutic intervention in vascular disease.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1303-z
       
  • Design and fabrication of devices for investigating cell-sheet stretch
    • Authors: Yang Liu; Yoshihiro Ojima; Masanobu Horie; Eiji Nagamori; Hideaki Fujita
      Abstract: Abstract Recently, many efforts have been made to investigate the cell stretch response through focal adhesions, which are usually utilized by cells cultured on elastic materials. However, not all stretch sensing is mediated through focal adhesions but from cell-cell contacts such as adherence junctions. To unveil the details of the stretch-sensing mechanism through cell-cell contacts, we developed a cell-sheet extension device for visualizing dynamic changes of individual living cells induced by external mechanical stretch. A cell-sheet is an ideal observation object, as it can be comprised merely of cells without any mechanical influences from external matrices while maintaining normal cell-cell adhesions as in the in vivo situation. Two microfluidic extension devices were designed and fabricated using a silicone elastomer, which were capable of extending a cell-sheet made from Caco-2 cells up to 1.3- and 1.5-fold from its original state. Based on the obtained results, we expected that such cell-sheet extension systems can be useful tools for understanding the mechanisms of cellular stretch sensing through cell-cell contacts in living cells.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1301-1
       
  • Joule heating effects on two-phase flows in dielectrophoresis microchips
    • Authors: Ying Yan; Dan Guo; Shizhu Wen
      Abstract: Abstract Joule heating is a significant phenomenon in dielectrophoresis-based microfluidic devices due to the local amplification of the electric field around electrodes. This leads to the temperature rising in microchannel which would influence the bioactivity of samples in the microchannel. In this paper, a numerical simulation model considering joule heating effect, electric field, and flow field is proposed to describe dynamic behaviors of two-phase flows of the dielectrophoresis microchip. The experimental methods are applied to verify the rationality of model and joule heating effects on microchips under both AC (Alternating current) and DC (Direct current) electric fields are investigated in detail. In numerical simulation part, the difference between AC and DC electric fields is compared. According to the results, it is found that with the same effective voltage, DC electric field can generate more joule heat and flow in the microchannel obtained higher temperature. This is caused by that the electric field strength around the electrodes in the DC electric field is stronger than that of the AC electric field. Secondly, effects of dispersed phase and continuous phase flow velocity are investigated and temperature will decrease with the increasing of flow velocity. Also, it is found that the Joule heating effects improve the droplet velocity based on the results. Finally, an infrared camera is applied to monitor the thermal characteristics of the microchannel to ensure that the numerical simulation is reasonable. These results are expected to provide useful guidance for future designs of dielectrophoresis-based microdevices that will avoid joule heating effects or take advantage of joule heating effects.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1209-9
       
  • Relationship analysis of speech communication between salivary cortisol
           levels and personal characteristics using the Smartphone Linked Stress
           Measurement (SLSM)
    • Authors: Seoyeon Choi; Yoosun Hwang; Joonchul Shin; Jung-Sik Yang; Hyo-Il Jung
      Abstract: Abstract Salivary cortisol is frequently used as a biomarker of stress. However, no study has used salivary cortisol to evaluate stress levels related to personal characteristics with a paper-based lateral flow assay (LFA) strip and a smartphone holder, and a smartphone at the same time during simulated speech communication. In this paper, we investigated variations in levels of salivary cortisol, communication apprehension, and the effects of self-efficacy via the Smartphone Linked Stress Measurement (SLSM) that we have recently reported. Saliva samples were collected by 48 participants (30 male and 18 female) at three moments: immediately before public speaking (basal measurement), immediately after public speaking (speech measurement), and forty minutes after taking a rest (relaxed measurement). Results from the questionnaire showed that salivary cortisol levels, communication apprehension, and self-efficacy were significantly correlated. Also, anxiety about speaking can raise their stress levels during their speeches, as reflected in their increased cortisol levels. Therefore, this study establishes the effects of self-efficacy on communication apprehension and salivary cortisol.
      PubDate: 2017-01-04
      DOI: 10.1007/s13206-016-1202-8
       
  • Automated nucleic acids purification from fecal samples on a microfluidic
           cartridge
    • Authors: JinSeok Kang; Chinsung Park; Jangwon Lee; Junghyun Namkung; Seung Yong Hwang; Yeon Seok Kim
      Abstract: Abstract In this paper, we describe the development of a microfluidic cartridge and its controlling device for fully automated nucleic acid purification from fecal samples. All steps of the standard complicated process, including fecal sample pretreatment, lysis, washing, and elution, were integrated into a microfluidic cartridge that could be operated with minimal hands-on time requirement. The performance of the microfluidic cartridge was evaluated using fecal samples spiked with bacteria or virus as well as fecal microbiome analysis. Microfluidic cartridge-based nucleic acid purification exhibited similar or even higher performance compared to commercialized products with respect to the concentration and purity of bacterial DNA or viral RNA. It also showed a similar or higher detection capa city for microbiome diversity in clinical fecal samples, as well as similar or higher extraction efficiency from such samples. The development of microfluidic cartridge-based fully automated platform for nucleic acid purification from fecal samples may be an important milestone towards developing a point-of-care test for rapid and simple diagnosis of gastroenteritis and gut microbiome analysis.
      PubDate: 2017-01-04
      DOI: 10.1007/s13206-016-1205-5
       
  • A simple and real-time sensing of human serum albumin using
           antibody-modified CNT-FET
    • Authors: Tae Hyun Kim
      Abstract: Abstract Microalbuminuria is a key indicator for the treatment of cardiovascular or cerebrovascular diseases and death. Therefore, sensitive and specific detection methods of HSA in biological fluids especially in low concentration are necessary in diagnosis and preventive medicine. Here, nanostructured electrical immunosensor is demonstrated for the rapid, sensitive and selective detection of human serum albumin (HSA), using single-walled carbon nanotube (swCNT) field-effect transistor (FETs) functionalized with monoclonal anti-human serum albumin (mAHSA) via simple coating. The selective binding of HSA with mAHSA on the surface of swCNT induces the electrostatic gating effect to the swCNT-FET. The resulting sensor exhibited high sensitivity toward HSA in real time, with good selectivity. The proposed method provides a powerful platform for a real time, sensitive and selective monitoring of HSA in biological fluids.
      PubDate: 2017-01-04
      DOI: 10.1007/s13206-016-1204-6
       
  • Serological tests for the diagnosis of infectious diseases
    • Authors: Soo-Kyung Kim; Sang-Hyun Hwang; Heung-Bum Oh
      Abstract: Abstract Recent automated systems for the serological tests employing chemiluminescence immunoassay or elec trochemiluminescent immunoassay give results within 30 minutes with high sensitivity and accuracy. However, false positive results are inevitable in serological screening for an infection due to the trade-off between sensitivity and specificity. A titration test has been used to confirm the screening results, but it is rarely performed. Instead, molecular tests are preferred in these days. Immunoassays were mostly qualitative in the past; however, quantitative kits have been developed and released on the market. The calibration step is necessary to report results quantitatively. As the calibration curve of an immunoassay is non-linear, four parameter or five parameter logistic models are adopted. Analytical measurement range (AMR) verification should be performed periodically at least every six months in order to report quantitative results. Quantitative reporting is feasible only within a linear range that is verified by AMR evaluation. A new concept of quantitative measurement/qualitative reporting in immunoassays is introduced. Quali tative reporting even after the quantitative measurement has the advantage that periodic AMR verification is not required. This category can be applied if the accurate quantitative value is not clinically significant. Lately, the market of point-of-care testing (POCT) is growing rapidly due to an increasing need. Nevertheless, the performance of the serological POCT kits is much lower than that of automated systems for central laboratories. This limits the broad utilization of POCT, and thus research for enhancing its performance should continue.
      PubDate: 2016-12-06
      DOI: 10.1007/s13206-016-0410-6
       
  • Development of GLP-1 secretagogue using microarray in enteroendocrine L
           cells
    • Authors: Kang-Hoon Kim; Hyeung-Jin Jang
      Abstract: Abstract Glucagon-like peptide-1 (GLP-1) plays glucose homeostasis and delays gastric empty. Because GLP-1 activates GLP-1 receptor and this activation recruits insulin secretion in pancreatic β-cells. This biological action applied therapeutic treatment for type 2 diabetes mellitus using GLP-1 analogues, exendin-4 and lilaglutide. Although therapeutic approaches of GLP-1 analogues are very effective as a hypoglycemic agents, its side effects are occurred in clinical study such as pancreatitis, autoimmune hepatitis and acute kidney injury. To solve critical side effects in therapeutic treatment, alternative ways are still developed. In this review, we introduce the character of taste receptors and taste receptor signaling. Because taste receptors and taste receptor signaling are able to induce GLP-1 release from exogenous molecules in enteroendocrine L cells. And how we find and develop that safe exogenous molecules to induce GLP-1 are in natural resources against side effects. We suggest that mRNA variants of taste receptors and taste receptor signaling molecules are briefly screening to find GLP-1 secretagogue in natural components including herbal medicines using biochip in enteroendocrine L cells.
      PubDate: 2016-11-29
      DOI: 10.1007/s13206-016-0403-5
       
 
 
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