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  Subjects -> CHEMISTRY (Total: 845 journals)
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    - CHEMISTRY (595 journals)
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CHEMISTRY (595 journals)                  1 2 3 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 8)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 26)
ACS Catalysis     Full-text available via subscription   (Followers: 34)
ACS Chemical Neuroscience     Full-text available via subscription   (Followers: 18)
ACS Combinatorial Science     Full-text available via subscription   (Followers: 23)
ACS Macro Letters     Full-text available via subscription   (Followers: 24)
ACS Medicinal Chemistry Letters     Full-text available via subscription   (Followers: 39)
ACS Nano     Full-text available via subscription   (Followers: 246)
ACS Photonics     Full-text available via subscription   (Followers: 11)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 21)
Acta Chemica Iasi     Open Access   (Followers: 2)
Acta Chimica Sinica     Full-text available via subscription   (Followers: 1)
Acta Chimica Slovaca     Open Access   (Followers: 1)
Acta Chromatographica     Full-text available via subscription   (Followers: 9)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 5)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 5)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 8)
Adsorption Science & Technology     Full-text available via subscription   (Followers: 5)
Advanced Functional Materials     Hybrid Journal   (Followers: 51)
Advanced Science Focus     Free   (Followers: 3)
Advances in Chemical Engineering and Science     Open Access   (Followers: 56)
Advances in Chemical Science     Open Access   (Followers: 13)
Advances in Chemistry     Open Access   (Followers: 15)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Enzyme Research     Open Access   (Followers: 9)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 16)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 9)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 21)
Advances in Nanoparticles     Open Access   (Followers: 15)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15)
Advances in Polymer Science     Hybrid Journal   (Followers: 41)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 17)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Science and Technology     Full-text available via subscription   (Followers: 12)
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 2)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 7)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 67)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 14)
American Journal of Chemistry     Open Access   (Followers: 27)
American Journal of Plant Physiology     Open Access   (Followers: 14)
American Mineralogist     Hybrid Journal   (Followers: 14)
Analyst     Full-text available via subscription   (Followers: 40)
Angewandte Chemie     Hybrid Journal   (Followers: 199)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 218)
Annales UMCS, Chemia     Open Access   (Followers: 1)
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 4)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 4)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 8)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 15)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antiviral Chemistry and Chemotherapy     Hybrid Journal  
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 7)
Applied Spectroscopy     Full-text available via subscription   (Followers: 23)
Applied Surface Science     Hybrid Journal   (Followers: 28)
Arabian Journal of Chemistry     Open Access   (Followers: 6)
ARKIVOC     Open Access   (Followers: 2)
Asian Journal of Biochemistry     Open Access   (Followers: 1)
Atomization and Sprays     Full-text available via subscription   (Followers: 4)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 2)
Avances en Quimica     Open Access   (Followers: 1)
Biochemical Pharmacology     Hybrid Journal   (Followers: 10)
Biochemistry     Full-text available via subscription   (Followers: 310)
Biochemistry Insights     Open Access   (Followers: 6)
Biochemistry Research International     Open Access   (Followers: 6)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 9)
Bioinspired Materials     Open Access   (Followers: 5)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access   (Followers: 1)
Biomacromolecules     Full-text available via subscription   (Followers: 19)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 10)
Biomedical Chromatography     Hybrid Journal   (Followers: 6)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 5)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 118)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 91)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 18)
Biosensors     Open Access   (Followers: 2)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 2)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 24)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 3)
Cakra Kimia (Indonesian E-Journal of Applied Chemistry)     Open Access  
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 3)
Canadian Journal of Chemistry     Hybrid Journal   (Followers: 10)
Canadian Mineralogist     Full-text available via subscription   (Followers: 4)
Carbohydrate Research     Hybrid Journal   (Followers: 26)
Carbon     Hybrid Journal   (Followers: 66)
Catalysis for Sustainable Energy     Open Access   (Followers: 7)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 8)
Catalysis Science and Technology     Free   (Followers: 7)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 8)
Cellulose     Hybrid Journal   (Followers: 7)
Cereal Chemistry     Full-text available via subscription   (Followers: 4)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 1)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 14)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 72)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 24)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Full-text available via subscription   (Followers: 20)
Chemical Reviews     Full-text available via subscription   (Followers: 181)
Chemical Science     Open Access   (Followers: 22)
Chemical Technology     Open Access   (Followers: 16)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 5)
Chemical Week     Full-text available via subscription   (Followers: 8)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 58)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 26)
ChemInform     Hybrid Journal   (Followers: 8)
Chemistry & Biodiversity     Hybrid Journal   (Followers: 6)
Chemistry & Biology     Full-text available via subscription   (Followers: 30)
Chemistry & Industry     Hybrid Journal   (Followers: 5)
Chemistry - A European Journal     Hybrid Journal   (Followers: 146)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 15)
Chemistry and Materials Research     Open Access   (Followers: 20)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Hybrid Journal   (Followers: 2)
Chemistry Letters     Full-text available via subscription   (Followers: 44)
Chemistry of Materials     Full-text available via subscription   (Followers: 255)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 9)
Chemistry World     Full-text available via subscription   (Followers: 22)
Chemistry-Didactics-Ecology-Metrology     Open Access   (Followers: 1)
ChemistryOpen     Open Access   (Followers: 2)
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 4)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 15)
Chemosensors     Open Access  
ChemPhysChem     Hybrid Journal   (Followers: 9)
ChemPlusChem     Hybrid Journal   (Followers: 2)
ChemTexts     Hybrid Journal  
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 10)
Chromatographia     Hybrid Journal   (Followers: 24)
Clay Minerals     Full-text available via subscription   (Followers: 10)
Cogent Chemistry     Open Access  
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 10)
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 7)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 4)
Combustion Science and Technology     Hybrid Journal   (Followers: 18)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Composite Interfaces     Hybrid Journal   (Followers: 6)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 2)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 1)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 9)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 12)
Computational Chemistry     Open Access   (Followers: 2)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 9)
Coordination Chemistry Reviews     Full-text available via subscription   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 5)
Crystal Structure Theory and Applications     Open Access   (Followers: 4)
CrystEngComm     Full-text available via subscription   (Followers: 13)
Current Catalysis     Hybrid Journal   (Followers: 2)
Current Metabolomics     Hybrid Journal   (Followers: 5)
Current Opinion in Colloid & Interface Science     Hybrid Journal   (Followers: 9)
Current Opinion in Molecular Therapeutics     Full-text available via subscription   (Followers: 17)
Current Research in Chemistry     Open Access   (Followers: 8)
Current Science     Open Access   (Followers: 61)
Dalton Transactions     Full-text available via subscription   (Followers: 23)
Detection     Open Access   (Followers: 2)
Developments in Geochemistry     Full-text available via subscription   (Followers: 2)
Diamond and Related Materials     Hybrid Journal   (Followers: 12)
Dislocations in Solids     Full-text available via subscription  
Doklady Chemistry     Hybrid Journal  
Drying Technology: An International Journal     Hybrid Journal   (Followers: 4)
Eclética Química     Open Access   (Followers: 1)
Ecological Chemistry and Engineering S     Open Access   (Followers: 3)
Ecotoxicology and Environmental Contamination     Open Access  
Educación Química     Open Access   (Followers: 1)
Education for Chemical Engineers     Hybrid Journal   (Followers: 5)
EJNMMI Radiopharmacy and Chemistry     Open Access  
Elements     Full-text available via subscription   (Followers: 3)
Environmental Chemistry     Hybrid Journal   (Followers: 7)
Environmental Chemistry Letters     Hybrid Journal   (Followers: 4)
Environmental Science & Technology Letters     Full-text available via subscription   (Followers: 5)

        1 2 3 | Last

Journal Cover BioChip Journal
  [SJR: 0.368]   [H-I: 12]   [0 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1976-0280 - ISSN (Online) 2092-7843
   Published by Springer-Verlag Homepage  [2352 journals]
  • Relationship analysis of speech communication between salivary cortisol
           levels and personal characteristics using the Smartphone Linked Stress
           Measurement (SLSM)
    • Authors: Seoyeon Choi; Yoosun Hwang; Joonchul Shin; Jung-Sik Yang; Hyo-Il Jung
      Pages: 101 - 107
      Abstract: Salivary cortisol is frequently used as a biomarker of stress. However, no study has used salivary cortisol to evaluate stress levels related to personal characteristics with a paper-based lateral flow assay (LFA) strip and a smartphone holder, and a smartphone at the same time during simulated speech communication. In this paper, we investigated variations in levels of salivary cortisol, communication apprehension, and the effects of self-efficacy via the Smartphone Linked Stress Measurement (SLSM) that we have recently reported. Saliva samples were collected by 48 participants (30 male and 18 female) at three moments: immediately before public speaking (basal measurement), immediately after public speaking (speech measurement), and forty minutes after taking a rest (relaxed measurement). Results from the questionnaire showed that salivary cortisol levels, communication apprehension, and self-efficacy were significantly correlated. Also, anxiety about speaking can raise their stress levels during their speeches, as reflected in their increased cortisol levels. Therefore, this study establishes the effects of self-efficacy on communication apprehension and salivary cortisol.
      PubDate: 2017-06-01
      DOI: 10.1007/s13206-016-1202-8
      Issue No: Vol. 11, No. 2 (2017)
  • Microfluidic biochips for simple impedimetric detection of thrombin based
           on label-free DNA aptamers
    • Authors: Taemin Lim; Seung Yong Lee; Jeongsuong Yang; Seung Yong Hwang; Yoomin Ahn
      Pages: 109 - 115
      Abstract: This paper presents a proof-of-concept microfluidic aptamer-based sensor for thrombin point-of-care testing using electrochemical impedance spectroscopy. The disposable biosensor is composed of a polydimethylsiloxane (PDMS) channel layer over a glass substrate. The substrate surface has an Au working electrode and a Pt reference/counter electrode. In this study, human thrombin was used as a biomarker for disease diagnosis. An unlabeled aptamer specific to thrombin was immobilized on a working electrode and electrochemical impedance was measured as thrombin was injected into the biosensor. Thrombin was detected by measuring change in impedance. The proposed biochip had a detection range of 0.1–100,000 ng mL−1 for thrombin concentration and a limit of detection of 0.1 ng mL−1. The results indicated that our biochip could be an effective tool for other molecular diagnostic systems.
      PubDate: 2017-06-01
      DOI: 10.1007/s13206-016-1203-7
      Issue No: Vol. 11, No. 2 (2017)
  • A simple and real-time sensing of human serum albumin using
           antibody-modified CNT-FET
    • Authors: Tae Hyun Kim
      Pages: 116 - 120
      Abstract: Microalbuminuria is a key indicator for the treatment of cardiovascular or cerebrovascular diseases and death. Therefore, sensitive and specific detection methods of HSA in biological fluids especially in low concentration are necessary in diagnosis and preventive medicine. Here, nanostructured electrical immunosensor is demonstrated for the rapid, sensitive and selective detection of human serum albumin (HSA), using single-walled carbon nanotube (swCNT) field-effect transistor (FETs) functionalized with monoclonal anti-human serum albumin (mAHSA) via simple coating. The selective binding of HSA with mAHSA on the surface of swCNT induces the electrostatic gating effect to the swCNT-FET. The resulting sensor exhibited high sensitivity toward HSA in real time, with good selectivity. The proposed method provides a powerful platform for a real time, sensitive and selective monitoring of HSA in biological fluids.
      PubDate: 2017-06-01
      DOI: 10.1007/s13206-016-1204-6
      Issue No: Vol. 11, No. 2 (2017)
  • Propagating behavior comparison of analytes and background electrolytes in
           a concentration polarization process
    • Authors: Longnan Li; Daejoong Kim
      Pages: 121 - 130
      Abstract: Concentration polarization (CP) is an electrochemical phenomenon related to the concentration gradient (under a transversal electric field) near an ion selective membrane surface. During concentration polarization process, quantitatively investigate the background electrolyte (BGE) concentration distribution is a very challenging problem. In this study, we conducted transient simulations within 2D micro-nanochannel domain to capture the dynamic nature of concentration polarization process and compared the propagating behavior of analytes and BGE. The simulation results show that the depletion area of chloride ion and analyte are both increase with time at ohmic and limiting region. The normalized depletion area (NDA) differences increases with decreasing BGE concentration. For the high applied voltage (overlimiting region), chloride ion and analyte at the anodic side microchannel are depleted everywhere that the NDA tends to 1 after an increasing step because of the electroconvection effect. This means there is no depletion area (DA) differences between two species in this plateau region. These results tell us the fluorescent ion indicator can’t show the exact BGE concentration distribution during CP process and we need to find new technique to visualize the concentration field.
      PubDate: 2017-06-01
      DOI: 10.1007/s13206-016-1110-y
      Issue No: Vol. 11, No. 2 (2017)
  • Development of a 2-chamber culture system for impedimetric monitoring of
           cell-cell interaction
    • Authors: Kin Fong Lei; Meng-Tsan Tsai; Ming-Hong Zhong; Chia-Hao Huang; Ngan-Ming Tsang; Ming-Yih Lee
      Pages: 139 - 145
      Abstract: In cancer research, study of cell-cell interaction is important to understand tumor initiation, progression, metastasis, and therapeutic resistance. Conventionally, transwell system was adopted and cell proliferation was quantified by end-point bio-assays. The operations are labor-intensive and time-consuming while studying of the dynamic cellular responses of cell-cell interaction. Although impedance measurement was suggested to be a promising technique to monitor cellular responses, electrodes cannot be integrated into the transwell for the measurement purpose. In this work, a 2-chamber culture system incorporated with impedance measurement technique was developed to quantitatively study cell-cell interaction. The chamber was composed of 2 sub-chambers separated with a barrier. By this design, two types of cells could be independently cultured and concurrently monitored under common medium supplied. Cell-cell interaction was demonstrated by aberrant cell proliferation induced by the EGF secreted from the transfected cells cultured on another sub-chamber. Real-time and non-invasive monitoring of cell-cell interaction was successfully demonstrated. This work provides a practical solution for monitoring the dynamic cellular responses of cell-cell interaction during the culture course. It is a reliable and convenient platform and facilitate more quantitative assessments in cancer research.
      PubDate: 2017-06-01
      DOI: 10.1007/s13206-016-1207-3
      Issue No: Vol. 11, No. 2 (2017)
  • Voltage regulation of retina neuron model with dynamic feedback for
           biological acquisition image
    • Authors: Ala’aDdin Al-Shidaifat; Sandeep Kumar; Hanjung Song
      Abstract: The investigation on biological activated imagers using standard CMOS processes has become continuous trend where silicon retina with central-plane image processing, small pixel sizes, large dynamic range and relatively low power consumption are required. This work proposes a voltage regulation of retina neuron model with dynamic feedback approach for biological acquisition image. The implementation of retina neuron circuit consists of conventional current-feedback event generator with the extension of proposed current mirror and dynamic feedback stage. The proposed neuron circuit achieves extremely high dynamic voltage range with respect to light intensity which help to detect biological acquisition image and could be beneficial for retinal prostheses. Moreover, individually modelling of photodiode using Verilog-A and device model is proposed for activation of current-feedback event generator. This modeling of photodiode permits to simple, compact and linear solution for pixel implementation. The proposed voltage controlled retina neuron circuit is implemented and fabricated using 0.18 μm Magnachip CMOS process. The spikes of output voltage are varied according to the inputs taken as control voltage and light intensity. As per the observation, read-out spikes of output voltage pulses provide more brightness level in the image pixels. The fabrication of proposed neuron circuit achieves less power consumption in nano-joule under dc supply of 3.3 V. The experimental result of output voltage is made good correlation with simulated one.
      PubDate: 2017-10-18
      DOI: 10.1007/s13206-017-1406-6
  • Gold nanoislands chip for Laser Desorption/Ionization (LDI) mass
    • Authors: Joo-Yoon Noh; Jo-Il Kim; Young Wook Chang; Jong-Min Park; Hyun-Woo Song; Min-Jung Kang; Jae-Chul Pyun
      Abstract: Gold nanoislands chip were developed as a solid matrix for the analysis of small molecules using laser desorption/ionization time-of-flight (LDIToF) mass spectrometry substituting the organic matrices which usually produce strong noise at low massto- charge ratio range (m/z<500). Gold nanoislands were simply prepared by (1) deposition of gold layer on highly P-doped silicon substrate followed by (2) heating. Physical properties of the gold nanoislands were analyzed by scanning electron microscope (SEM), atomic force microscopy (AFM) analysis to characterize the morphology, diameter, thickness, and surface density of the nanoislands which were comparatively analyzed in conjunction with LDI-ToF mass spectrometry. In this work, optimal size of the nanoislands were selected for LDI-ToF mass spectrometry, and gold nanoislands chip were applied to the quantitative and simultaneous detection of small analytes (amino acids) with various chemical and physical properties (polar, nonpolar, acidic, and basic).
      PubDate: 2017-09-08
      DOI: 10.1007/s13206-017-1310-0
  • Microfabricated cell culture system for the live cell observation of the
           multilayered proliferation of undifferentiated HT-29 cells
    • Authors: Young Lee; Je-Kyun Park
      Abstract: In this paper, we describe a microfabricated cell culture system (MCCS) for the microscopic live observation of cellular behavior in a reconstituted tissue-like microenvironment. The MCCS was used as a novel tool for tracking the proliferation of undifferentiated HT-29 cells. A wall structure of gelled extracellular matrix (ECM) protein was constructed inside the MCCS to establish a tissue-like microenvironment of the intestinal tissue. The ECM wall consisting of type I collagen retained the structural integrity during the gelation process and provided the adhesion surface for the HT-29 cells. We carried out the microscopic live observation of the proliferation of HT-29 cells seeded with various concentrations. The multilayered growth of HT-29 cells in the undifferentiated state was monitored without cell fixation, which was a necessary process in a conventional in vitro cell culture. The live tracking of cell culture also recorded the contraction of cell culture morphology during the initial period of cultivation. This approach is helpful for the establishment of an in vitro model for the live observation study of intestinal epithelial differentiation in an in vivo-like microenvironment.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1407-5
  • Prenatally diagnosed TTN mutation with repeated bilateral club foot by
           whole exome sequencing
    • Authors: So Hyun Shim; Ji Youn Kim; Se Ra Sung; Ji Eun Park; Yun Jeong Shin; Sung Han Shim; Dong Hyun Cha
      Abstract: With significant advances in genomic technologies over recent years, Next-generation sequencing (NGS) has become an important tool not only for gene discovery and research but also for clinical genetic diagnosis of rare disorders and single-gene-disorder analysis. Many hundreds of single-gene disorders have been described that can manifest prenatally and will comprise a large portion of undiagnosed, chromosomally normal cases but underlying cause of the abnormality still remains unknown for the large majority of cases with an abnormal ultrasound. However, there has been little experience and study in using trio whole exome sequencing (WES) for prenatal diagnosis. In this report the NGS method was applied for the prenatal genetic diagnosis of unidentified life-threatening phenotype in one family and confirmed the pattern of inheritance of titinopathy as well as two novel TTNgene variations. The report presented in these pages offers a unique and valuable perspective on relevance and feasibility of NGS in prenatal diagnosis field.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1309-6
  • Integrated analysis of miRNA and mRNA expression profiles in human
           endothelial cells exposed to fisetin
    • Authors: Seung Eun Lee; Hye Rim Park; Hong Duck Yun; Hyemi Kim; Young-Ho Jin; Jeong-Je Cho; Hyun-Jong Ahn; Cheung-Seog Park; Yong Seek Park
      Abstract: MicroRNAs (miRNAs) play vital regulatory roles in various biological processes including cell differentiation, proliferation and apoptosis. Several studies have examined the expression profiles of miRNAs in response to different drugs and chemicals and suggested that expression patterns were associated with the effects of drugs and chemicals. Fisetin, a polyphenol found in a wide range of plants, has been reported to exhibit numerous biological activities, such as antioxidant, anti-inflammatory, antiangiogenic, and antiproliferative properties. In the present study, we evaluated whether fisetin regulates miRNA and mRNA expression. Using microarray analysis, we measured global miRNA and mRNA expression in human endothelial cells treated with 10 μM of fisetin for 24 h. Using several bioinformatic systems, we determined the signatures of potential biological processes and signaling pathways such as cell migration, apoptotic signaling pathway, cell motility, and cardiovascular system development. Our findings provide insight into the molecular mechanisms of action of fisetin and can be used to improve therapeutic intervention in vascular diseases.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1308-7
  • Improvement of droplet speed and stability in electrowetting on dielectric
           devices by surface polishing
    • Authors: Hamidreza Shirinkami; Jiman Kim; Choonghee Lee; Hee Chan Kim; Honggu Chun
      Abstract: This paper reports on a technique that significantly improves the performance of open-plate electrowetting on dielectric (EWOD) devices by using chemical mechanical polishing (CMP) to achieve surface planarization. In EWOD devices, trenches are inevitably generated during fabrication, and these can have a considerable effect on droplet manipulation. Such artefacts lead to a local intensification in the electric field that not only hinders the droplet movement due to a partially increased adhesion force, but also limits the maximum applicable potential by increasing the likelihood of a dielectric breakdown. We conducted theoretical and experimental studies on the effect of trenches on the performance of both unpolished and polished EWOD devices. Various combinations of the droplet volume and electrode size were tested, and our proposed technique resulted in a notable improvement for all cases. For instance, when operating at 100 V, a 3.5 μL droplet could be translated at 70 mm/s on the polished EWOD device, which is twice as fast as that for its unpolished counterpart at the same voltage. Also, the maximum applicable potential resulting in a dielectric breakdown of the polished EWOD device was measured to be 27% higher than that of a conventional device.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1408-4
  • Controlled production of monodisperse polycaprolactone microspheres using
           flow-focusing microfluidic device
    • Authors: Hyukjin J. Kwon; Suhyeon Kim; Sungwook Kim; Ju Hee Kim; Geunbae Lim
      Abstract: These days, biodegradable microsphere polymers have been attracting increasing interest as a cosmetic injectable filler. Particularly, polycaprolactone (PCL) microspheres are well known for their safety and long degradation time. However, these microspheres are usually produced by the conventional stirring method, which has an inherent drawback related to the size control of the microspheres; accurate size control is critical for the use of these microspheres as a filler. Here, we demonstrate the fabrication of monodisperse PCL microspheres in the size range of 26.6-161 μm using a flow-focusing microfluidic device. The acquired coefficient of variation of the solidified microspheres is approximately 4.5%; thus, these microspheres meet the requirement of being monodisperse. The study results show the feasibility of manufacturing PCL microspheres using a microfluidic device, and these microspheres have better morphological characteristics, thereby reducing pain and infection after their injection into the skin. Furthermore, the specific target size of the solidified microspheres is met. Therefore, undesired outcomes after microsphere injection through the dermis, such as phagocytosis and inflammatory reactions, are less likely to occur.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1306-9
  • Flexible and implantable capacitive microelectrode for bio-potential
    • Authors: Seung Min Lee; Hang Jin Byeon; Bong Hoon Kim; Jungyup Lee; Ji Yoon Jeong; Joong Hoon Lee; Jin-Hee Moon; Cheolsoo Park; Hyuk Choi; Sang-Hoon Lee; Kwang-Ho Lee
      Abstract: Electrodes should be adhered onto the body steadily under motion, and implanted stably into the tissue without any damages while maintaining conformal contact. Although most electrodes are fabricated with biocompatible materials, they should be shielded from tissues to prevent mechanical delamination from the device itself and to avoid adverse effects such as irritation, allergic reactions, or inflammation. Herein, we demonstrate a simple process for the development of a flexible and implantable capacitive electrode based on a minimal incision accessible design with polyimide (PI) and Gold/Titanium (Au/Ti) layers and completely encapsulated in a polydimethylsiloxane (PDMS) substrate. Electrodes of three different sizes (recording site diameters of 1.8 mm, 2.8 mm, and 3.8 mm, respectively) were fabricated and examined in this work. Electrocardiography (ECG) was recorded in the dorsal area of the rat for 4 weeks for biological signal checkup. We obtained stable and robust ECG signals owing to the intrinsic property of capacitive coupling, with almost no leakage current compared to the direct contact electrode for the applied current over the range of 0 to 10 mA. These results indicate that our electrode can be used to detect bio-signals effectively in the long term, and can play a role in electroceuticals in the near future.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1304-y
  • miRNA-mediated expression switch of cell adhesion genes driven by
           microcirculation in chip
    • Authors: Timur R Samatov; Vladimir V Galatenko; Nadezhda V Senyavina; Alexey V Galatenko; Maxim Yu Shkurnikov; Svetlana A Tonevitskaya; Dmitry A Sakharov; Uwe Marx; Hermann Ehrlich; Udo Schumacher; Alexander G Tonevitsky
      Abstract: Changes in cell adhesion molecule (CAM) expression and miRNAs regulating them are known to be involved in malignant progression in colon cancer. We investigated expression profiles of CAM genes and non-coding RNAs in CaCo2 colon cancer cells in static culture and under dynamic flow conditions perfused in microfluidic chip emulating physiological microenvironment. We incubated monolayers of CaCo2 cells in Transwell® units either under static conditions or under flow in a microfluidic chip. We identified 7 up-regulated CAM genes (CD44, CDH7, CEACAM5, CEACAM6, CYR61, L1CAM and VCAN), 7 down-regulated genes (COL12A1, FGA, FGB, FGG, GJA1, ITGA5 and LAMA1) and 69 miRNAs targeting them under the influence of microcirculation. The revealed network comprised CAM genes known to interact with each other and 13 miRNAs simultaneously regulating more than one of them. The discovered regulatory network comprising CAM genes and miRNAs is likely involved in normal functioning of intestine epithelium as well as in cancer progression.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1305-x
  • Recent advances in the detection of methicillin resistant Staphylococcus
           aureus (MRSA)
    • Authors: John Hulme
      Abstract: According to the World Health Organization (WHO) 20% of all documented S. aureus infections are attributable to MRSA, although for some developing countries this value can exceed 80%. Thus the rapid and accurate detection of MRSA in low resource settings (LDR) is becoming essential. Yet conventional microbial detection methods take from 1-5 days to identify MRSA. Recently, new types of automated laboratory methods as well as advances in nucleic acid testing, microfluidic technology, immunosensors, biosensors and point of care testing have reduced the time to detection to <1 hr. This review examines the current limitations and advances in methodologies employed in the rapid detection of MRSA.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-016-1201-9
  • Three-dimensional in vitro gut model on a villi-shaped collagen scaffold
    • Authors: Banya Yi; Kyu Young Shim; Sang Keun Ha; Jeonghun Han; Hong-Hoa Hoang; Inwook Choi; Sungsu Park; Jong Hwan Sung
      Abstract: A three-dimensional (3D) tissue architecture provides essential cellular environmental cues for cell differentiation. We previously reported that Caco-2 human intestinal epithelial cells grown on a 3D villi-shaped scaffold composed of collagen created a tissue structure resembling the human intestinal villi. Here, we compared the absorptive and metabolic properties of Caco-2 cells cultured on the 3D villi with those in 2D monolayer. Cell growth was higher in the 3D villi model compared with the 2D monolayer, probably owing to an increase in the available surface area. Barrier functions were more in vivo-like in the 3D villi model in association with reduced expression of tight junction and transporter proteins. The specific activities of the metabolic enzyme and intestinal epithelial differentiation marker, alkaline phosphatase, were improved in the 3D villi model, whereas aminopeptidase activity was comparable in the two models. These results suggest that the 3D villi scaffold induces physiological changes in Caco-2 cells, related to the absorption and metabolism of drugs. This 3D villi model may serve as an alternative and improved in vitro gut model for studying drug metabolism and transport.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1307-8
  • miRHunter: A tool for predicting microRNA precursors based on combined
           computational method
    • Authors: Insong Koh; Ki-Bong Kim
      Abstract: MicroRNAs (miRNAs) are small endogenous non-coding RNAs known to post-transcriptionally regulate gene expression in a broad range of organism. Since the discovery of the very first miRNAs, lin-4 and let-7, computational methods have been indispensable tools that complement experimental approaches to understand the biology of miRNAs. In this article, we introduce a web-based computational tool, miRHunter, that identifies potential miRNA precursors (pre-miRNAs) in the genomic sequences by using a combined computational method. The method coupled ab initio method with homology-based and hairpin structure-based methods. The miRHunter consists of five modules: 1) a preprocessing module, 2) an evolutionary conservation filter module, 3) a hairpin structure filter module, 4) a support vector machine module that evaluates preliminary pre-miRNA candidates derived from the previous two filtering modules, and 5) a post-processing module. The miRHunter system yielded the following average test results: 96.16%/93.23%, 96.00%/94.68%, and 95.87%/93.57% which are sensitivity (Sn) and specificity (Sp) for animal, plant, and overall categories respectively. The miRHunter system can complement experimental methods and allow wetlab researchers to screen long sequences for putative miRNAs as well as pre-testing miRNAs of interest. The microarray profiling experiments have supported that the clusters of proximal pairs of miRNAs are generally coexpressed. Therefore, the clustering or spatial localization information will be used to improve the accuracy of our system in further work. The miRHunter is available at
      PubDate: 2017-05-15
      DOI: 10.1007/s13206-017-1210-3
  • PDMS microchannel surface modification with Teflon for algal lipid
    • Authors: Jae Woo Park; Sangcheol Na; Myeongwoo Kang; Sang Jun Sim; Noo Li Jeon
      Abstract: This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs without background signal from absorbed dye molecules on PDMS microchannel. We confirmed that contact angle of Teflon-coated PDMS (116.4°) is higher than that of unmodified PDMS (106.1°) and thus more hydrophobic. Furthermore, Teflon-coated PDMS surface had ~80% of oxygen transfer rate compared to that of native PDMS and good transparency in all visible light regions. Based on these characteristics, we successfully validated the visualization and quantification of intracellular lipid droplets in microalgae C. reinhardtii using BODIPY. We believe that our new method will expand microfluidic applications on characterization of biological lipid with fluorescence probes and biochemical markers.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1302-0
  • Application of paper EWOD (electrowetting-on-dielectrics) chip: Protein
           tryptic digestion and its detection using MALDI-TOF mass spectrometry
    • Authors: Inae Jang; Hyojin Ko; Gwangro You; Hyunji Lee; Seunghwi Paek; Heedo Chae; Jae Hwan Lee; Sunkyung Choi; Oh-Sun Kwon; Kwanwoo Shin; Han Bin Oh
      Abstract: A paper-based open EWOD chip was used for protein tryptic digestion, and the resulting peptides were analyzed using MALDI-TOF mass spectrometry to identify the proteins. Although on-chip protein digestion was previously demonstrated on a glass-based EWOD platform, this is the first report to show that a paper-based EWOD can also be used for protein digestion and further analysis by mass spectrometry. A number of protein digestion processes, i.e., disulfide bond reduction, alkylation, buffering, and tryptic digestion, can be successfully conducted on the paper-based EWOD chip. Furthermore, a “Y”-shaped junction design was shown to be effective in successfully manipulating a droplet for protein digestion. A paper-based EWOD platform offers a cheap and versatile avenue for biological applications.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-016-1208-2
  • Affinity characteristic of terminal sequence in vancomycin resistant
           Enterococcus (VRE) membrane peptides on nanobiosensor chip using localized
           surface plasmon resonance
    • Authors: Kyungmin Lee; Yo-Han Kim; Hunsang Jung; Hyun Ho Lee
      Abstract: In this study, specific binding affinity between vancomycin (VAN) conjugated 40-50 nm gold nanoparticles (Au NPs) implemented on a sensor chip and pentapeptide mimicking pathogenic VAN resistant Enterococcus (VRE) wall membrane was employed as nanosensor format based on localized surface plasmon resonance (LSPR). Two pentapeptides, which were also anchored to 5 nm Au NPs, having two different terminal sequences of d-Ala-d-Ala and d-Ala-d-Lac respectively were compared in specific binding affinity toward the VAN conjugated nanosensor. Binding affinity difference identified by the LSPR characteristic in the nanosensor was shown in absorbance intensity and absorption wavelength shift between the two different terminal pentapeptides. The absorbance intensity clearly indicated that the pentapeptide having terminal d-Ala-d-Lac showed less affinity toward VAN conjugated nanosensor chip than that having terminal d-Ala-d-Ala. It indicates that the VRE itself has relatively weak binding strength towards the VAN, which may be used as a probe molecule for the VRE. Therefore, in order to design a VRE sensor using the VAN affinity, an indirect LSPR method can be devised instead of direct LSPR method.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-016-1206-4
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