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  Subjects -> CHEMISTRY (Total: 871 journals)
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CHEMISTRY (610 journals)                  1 2 3 4 | Last

Showing 1 - 200 of 735 Journals sorted alphabetically
2D Materials     Hybrid Journal   (Followers: 13)
Accreditation and Quality Assurance: Journal for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 26)
ACS Catalysis     Full-text available via subscription   (Followers: 42)
ACS Chemical Neuroscience     Full-text available via subscription   (Followers: 20)
ACS Combinatorial Science     Full-text available via subscription   (Followers: 20)
ACS Macro Letters     Full-text available via subscription   (Followers: 25)
ACS Medicinal Chemistry Letters     Full-text available via subscription   (Followers: 40)
ACS Nano     Full-text available via subscription   (Followers: 264)
ACS Photonics     Full-text available via subscription   (Followers: 13)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 23)
Acta Chemica Iasi     Open Access   (Followers: 2)
Acta Chimica Sinica     Full-text available via subscription   (Followers: 1)
Acta Chimica Slovaca     Open Access   (Followers: 1)
Acta Chimica Slovenica     Open Access  
Acta Chromatographica     Full-text available via subscription   (Followers: 8)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 6)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 5)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 8)
Adsorption Science & Technology     Full-text available via subscription   (Followers: 5)
Advanced Functional Materials     Hybrid Journal   (Followers: 54)
Advanced Science Focus     Free   (Followers: 5)
Advances in Chemical Engineering and Science     Open Access   (Followers: 64)
Advances in Chemical Science     Open Access   (Followers: 15)
Advances in Chemistry     Open Access   (Followers: 20)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 19)
Advances in Drug Research     Full-text available via subscription   (Followers: 21)
Advances in Enzyme Research     Open Access   (Followers: 9)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 7)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 15)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 9)
Advances in Materials Physics and Chemistry     Open Access   (Followers: 25)
Advances in Nanoparticles     Open Access   (Followers: 15)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15)
Advances in Polymer Science     Hybrid Journal   (Followers: 43)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 17)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 18)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Science and Technology     Full-text available via subscription   (Followers: 12)
African Journal of Bacteriology Research     Open Access  
African Journal of Chemical Education     Open Access   (Followers: 3)
African Journal of Pure and Applied Chemistry     Open Access   (Followers: 7)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Al-Kimia : Jurnal Penelitian Sains Kimia     Open Access  
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 61)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 15)
American Journal of Chemistry     Open Access   (Followers: 29)
American Journal of Plant Physiology     Open Access   (Followers: 11)
American Mineralogist     Hybrid Journal   (Followers: 15)
Analyst     Full-text available via subscription   (Followers: 38)
Angewandte Chemie     Hybrid Journal   (Followers: 165)
Angewandte Chemie International Edition     Hybrid Journal   (Followers: 236)
Annales UMCS, Chemia     Open Access  
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 5)
Annual Reports in Computational Chemistry     Full-text available via subscription   (Followers: 3)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 4)
Annual Reports Section B (Organic Chemistry)     Full-text available via subscription   (Followers: 9)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antiviral Chemistry and Chemotherapy     Hybrid Journal   (Followers: 1)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 8)
Applied Spectroscopy     Full-text available via subscription   (Followers: 22)
Applied Surface Science     Hybrid Journal   (Followers: 31)
Arabian Journal of Chemistry     Open Access   (Followers: 5)
ARKIVOC     Open Access   (Followers: 1)
Asian Journal of Biochemistry     Open Access   (Followers: 1)
Atomization and Sprays     Full-text available via subscription   (Followers: 4)
Australian Journal of Chemistry     Hybrid Journal   (Followers: 7)
Autophagy     Hybrid Journal   (Followers: 2)
Avances en Quimica     Open Access  
Biochemical Pharmacology     Hybrid Journal   (Followers: 10)
Biochemistry     Full-text available via subscription   (Followers: 333)
Biochemistry Insights     Open Access   (Followers: 6)
Biochemistry Research International     Open Access   (Followers: 6)
BioChip Journal     Hybrid Journal  
Bioinorganic Chemistry and Applications     Open Access   (Followers: 9)
Bioinspired Materials     Open Access   (Followers: 5)
Biointerface Research in Applied Chemistry     Open Access   (Followers: 2)
Biointerphases     Open Access   (Followers: 1)
Biology, Medicine, & Natural Product Chemistry     Open Access   (Followers: 1)
Biomacromolecules     Full-text available via subscription   (Followers: 20)
Biomass Conversion and Biorefinery     Partially Free   (Followers: 10)
Biomedical Chromatography     Hybrid Journal   (Followers: 7)
Biomolecular NMR Assignments     Hybrid Journal   (Followers: 3)
BioNanoScience     Partially Free   (Followers: 5)
Bioorganic & Medicinal Chemistry     Hybrid Journal   (Followers: 120)
Bioorganic & Medicinal Chemistry Letters     Hybrid Journal   (Followers: 84)
Bioorganic Chemistry     Hybrid Journal   (Followers: 10)
Biopolymers     Hybrid Journal   (Followers: 18)
Biosensors     Open Access   (Followers: 2)
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
Bitácora Digital     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Bulletin of the Chemical Society of Ethiopia     Open Access   (Followers: 2)
Bulletin of the Chemical Society of Japan     Full-text available via subscription   (Followers: 24)
Bulletin of the Korean Chemical Society     Hybrid Journal   (Followers: 1)
C - Journal of Carbon Research     Open Access   (Followers: 3)
Cakra Kimia (Indonesian E-Journal of Applied Chemistry)     Open Access  
Canadian Association of Radiologists Journal     Full-text available via subscription   (Followers: 2)
Canadian Journal of Chemistry     Hybrid Journal   (Followers: 10)
Canadian Mineralogist     Full-text available via subscription   (Followers: 6)
Carbohydrate Research     Hybrid Journal   (Followers: 26)
Carbon     Hybrid Journal   (Followers: 71)
Catalysis for Sustainable Energy     Open Access   (Followers: 7)
Catalysis Reviews: Science and Engineering     Hybrid Journal   (Followers: 7)
Catalysis Science and Technology     Free   (Followers: 8)
Catalysis Surveys from Asia     Hybrid Journal   (Followers: 3)
Catalysts     Open Access   (Followers: 9)
Cellulose     Hybrid Journal   (Followers: 7)
Cereal Chemistry     Full-text available via subscription   (Followers: 4)
ChemBioEng Reviews     Full-text available via subscription   (Followers: 1)
ChemCatChem     Hybrid Journal   (Followers: 8)
Chemical and Engineering News     Free   (Followers: 17)
Chemical Bulletin of Kazakh National University     Open Access  
Chemical Communications     Full-text available via subscription   (Followers: 73)
Chemical Engineering Research and Design     Hybrid Journal   (Followers: 26)
Chemical Research in Chinese Universities     Hybrid Journal   (Followers: 3)
Chemical Research in Toxicology     Full-text available via subscription   (Followers: 22)
Chemical Reviews     Full-text available via subscription   (Followers: 182)
Chemical Science     Open Access   (Followers: 23)
Chemical Technology     Open Access   (Followers: 21)
Chemical Vapor Deposition     Hybrid Journal   (Followers: 5)
Chemical Week     Full-text available via subscription   (Followers: 7)
Chemie in Unserer Zeit     Hybrid Journal   (Followers: 56)
Chemie-Ingenieur-Technik (Cit)     Hybrid Journal   (Followers: 24)
ChemInform     Hybrid Journal   (Followers: 8)
Chemistry & Biodiversity     Hybrid Journal   (Followers: 7)
Chemistry & Biology     Full-text available via subscription   (Followers: 32)
Chemistry & Industry     Hybrid Journal   (Followers: 6)
Chemistry - A European Journal     Hybrid Journal   (Followers: 151)
Chemistry - An Asian Journal     Hybrid Journal   (Followers: 16)
Chemistry and Materials Research     Open Access   (Followers: 20)
Chemistry Central Journal     Open Access   (Followers: 4)
Chemistry Education Research and Practice     Free   (Followers: 5)
Chemistry in Education     Open Access   (Followers: 9)
Chemistry International     Hybrid Journal   (Followers: 2)
Chemistry Letters     Full-text available via subscription   (Followers: 44)
Chemistry of Materials     Full-text available via subscription   (Followers: 243)
Chemistry of Natural Compounds     Hybrid Journal   (Followers: 9)
Chemistry World     Full-text available via subscription   (Followers: 19)
Chemistry-Didactics-Ecology-Metrology     Open Access   (Followers: 1)
ChemistryOpen     Open Access   (Followers: 1)
Chemkon - Chemie Konkret, Forum Fuer Unterricht Und Didaktik     Hybrid Journal  
Chemoecology     Hybrid Journal   (Followers: 4)
Chemometrics and Intelligent Laboratory Systems     Hybrid Journal   (Followers: 14)
Chemosensors     Open Access  
ChemPhysChem     Hybrid Journal   (Followers: 11)
ChemPlusChem     Hybrid Journal   (Followers: 2)
ChemTexts     Hybrid Journal  
CHIMIA International Journal for Chemistry     Full-text available via subscription   (Followers: 2)
Chinese Journal of Chemistry     Hybrid Journal   (Followers: 6)
Chinese Journal of Polymer Science     Hybrid Journal   (Followers: 11)
Chromatographia     Hybrid Journal   (Followers: 24)
Chromatography     Open Access   (Followers: 2)
Chromatography Research International     Open Access   (Followers: 6)
Clay Minerals     Full-text available via subscription   (Followers: 10)
Cogent Chemistry     Open Access  
Colloid and Interface Science Communications     Open Access  
Colloid and Polymer Science     Hybrid Journal   (Followers: 11)
Colloids and Interfaces     Open Access  
Colloids and Surfaces B: Biointerfaces     Hybrid Journal   (Followers: 6)
Combinatorial Chemistry & High Throughput Screening     Hybrid Journal   (Followers: 5)
Combustion Science and Technology     Hybrid Journal   (Followers: 22)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal   (Followers: 2)
Composite Interfaces     Hybrid Journal   (Followers: 7)
Comprehensive Chemical Kinetics     Full-text available via subscription   (Followers: 1)
Comptes Rendus Chimie     Full-text available via subscription  
Comptes Rendus Physique     Full-text available via subscription   (Followers: 1)
Computational and Theoretical Chemistry     Hybrid Journal   (Followers: 9)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 11)
Computational Chemistry     Open Access   (Followers: 2)
Computers & Chemical Engineering     Hybrid Journal   (Followers: 10)
Coordination Chemistry Reviews     Full-text available via subscription   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Corrosion Series     Full-text available via subscription   (Followers: 6)
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 5)
Croatica Chemica Acta     Open Access  
Crystal Structure Theory and Applications     Open Access   (Followers: 4)
CrystEngComm     Full-text available via subscription   (Followers: 13)
Current Catalysis     Hybrid Journal   (Followers: 2)
Current Chromatography     Hybrid Journal  
Current Green Chemistry     Hybrid Journal  
Current Metabolomics     Hybrid Journal   (Followers: 5)
Current Microwave Chemistry     Hybrid Journal  
Current Opinion in Colloid & Interface Science     Hybrid Journal   (Followers: 9)
Current Opinion in Molecular Therapeutics     Full-text available via subscription   (Followers: 14)
Current Research in Chemistry     Open Access   (Followers: 8)
Current Science     Open Access   (Followers: 70)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Dalton Transactions     Full-text available via subscription   (Followers: 23)
Detection     Open Access   (Followers: 2)
Developments in Geochemistry     Full-text available via subscription   (Followers: 2)
Diamond and Related Materials     Hybrid Journal   (Followers: 12)
Dislocations in Solids     Full-text available via subscription  
Doklady Chemistry     Hybrid Journal  

        1 2 3 4 | Last

Journal Cover BioChip Journal
  [SJR: 0.368]   [H-I: 12]   [0 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1976-0280 - ISSN (Online) 2092-7843
   Published by Springer-Verlag Homepage  [2352 journals]
  • Design and fabrication of devices for investigating cell-sheet stretch
    • Authors: Yang Liu; Yoshihiro Ojima; Masanobu Horie; Eiji Nagamori; Hideaki Fujita
      Pages: 173 - 179
      Abstract: Recently, many efforts have been made to investigate the cell stretch response through focal adhesions, which are usually utilized by cells cultured on elastic materials. However, not all stretch sensing is mediated through focal adhesions but from cell-cell contacts such as adherence junctions. To unveil the details of the stretch-sensing mechanism through cellcell contacts, we developed a cell-sheet extension device for visualizing dynamic changes of individual living cells induced by external mechanical stretch. A cell-sheet is an ideal observation object, as it can be comprised merely of cells without any mechanical influences from external matrices while maintaining normal cell-cell adhesions as in the in vivo situation. Two microfluidic extension devices were designed and fabricated using a silicone elastomer, which were capable of extending a cell-sheet made from Caco-2 cells up to 1.3- and 1.5-fold from its original state. Based on the obtained results, we expected that such cell-sheet extension systems can be useful tools for understanding the mechanisms of cellular stretch sensing through cell-cell contacts in living cells.
      PubDate: 2017-09-01
      DOI: 10.1007/s13206-017-1301-1
      Issue No: Vol. 11, No. 3 (2017)
  • Integrated miRNA and mRNA expression profiling in response to eriodictyol
           in human endothelial cells
    • Authors: Seung Eun Lee; Hye Rim Park; Hong Duck Yun; Jeong-Je Cho; Hyun-Jong Ahn; Cheung-Seog Park; Yong Seek Park
      Pages: 188 - 195
      Abstract: Eriodictyol, a flavonoid commonly found in citrus fruits, shows neurotrophic, antioxidant, anti- inflammatory, and anti-cancer effects under diverse pathophysiological conditions such as vascular diseases. In this study, we evaluated whether eriodictyol modulates miRNA and mRNA expression. Using microarray analysis, we examined miRNA and mRNA expression in human endothelial cells treated with 10 μM of eriodictyol for 24 h. Using numerous bioinformatic systems, we evaluated the signatures of the potential biological processes and signaling pathways. Our results provide insight into the underlying molecular mechanisms of action of eriodictyol and suggest that eriodictyol can be used for therapeutic intervention in vascular disease.
      PubDate: 2017-09-01
      DOI: 10.1007/s13206-017-1303-z
      Issue No: Vol. 11, No. 3 (2017)
  • Joule heating effects on two-phase flows in dielectrophoresis microchips
    • Authors: Ying Yan; Dan Guo; Shizhu Wen
      Pages: 196 - 205
      Abstract: Joule heating is a significant phenomenon in dielectrophoresis-based microfluidic devices due to the local amplification of the electric field around electrodes. This leads to the temperature rising in microchannel which would influence the bioactivity of samples in the microchannel. In this paper, a numerical simulation model considering joule heating effect, electric field, and flow field is proposed to describe dynamic behaviors of two-phase flows of the dielectrophoresis microchip. The experimental methods are applied to verify the rationality of model and joule heating effects on microchips under both AC (Alternating current) and DC (Direct current) electric fields are investigated in detail. In numerical simulation part, the difference between AC and DC electric fields is compared. According to the results, it is found that with the same effective voltage, DC electric field can generate more joule heat and flow in the microchannel obtained higher temperature. This is caused by that the electric field strength around the electrodes in the DC electric field is stronger than that of the AC electric field. Secondly, effects of dispersed phase and continuous phase flow velocity are investigated and temperature will decrease with the increasing of flow velocity. Also, it is found that the Joule heating effects improve the droplet velocity based on the results. Finally, an infrared camera is applied to monitor the thermal characteristics of the microchannel to ensure that the numerical simulation is reasonable. These results are expected to provide useful guidance for future designs of dielectrophoresis-based microdevices that will avoid joule heating effects or take advantage of joule heating effects.
      PubDate: 2017-09-01
      DOI: 10.1007/s13206-017-1209-9
      Issue No: Vol. 11, No. 3 (2017)
  • Method for the differentiation of nine species of Sebastes with
           fluorescence melting curve analysis and dual-labeled probes
    • Authors: Eun Soo Noh; Young Sam Kim; Eun Mi Kim; Jung Youn Park; Jung-Ha Kang
      Pages: 206 - 212
      Abstract: Substitution of a high-value fish species and their products with a low-value species has recently emerged as a problem in the fishery industry, and the need for development of analysis tools for the identification of different species has been discussed globally. The genus Sebastes is the most species-rich of the family Scorpaenidae, and many of the species of Sebastes are commercially and ecologically important, particularly in the North Pacific. They exhibit high morphological similarity among species, and are difficult to distinguish visually. In this study, we selected nine common Sebastes species (S. pachycephalus, S. fasciatus, S. inermis, S. oblongus, S. owstoni, S. hubbsi, S. schlegeli, S. vulpes, and S. thompsoni) for development of a differentiation assay. Fluorescence melting curve analysis (FMCA) was used to distinguish the nine species of Sebastes with single locked nucleic acids (LNA). Dual-labeled probes were designed from the mitochondrial cytochrome c oxidase subunit I gene to detect each species and hybridize with the target sequence. In addition, we successfully multiplex analyzed three species of Sebastes using the fluorescent dyes FAM, HEX, and Cy5. The LNA-based FMCA method we developed is a powerful tool for the identification of nine different species of Sebastes.
      PubDate: 2017-09-01
      DOI: 10.1007/s13206-017-1402-x
      Issue No: Vol. 11, No. 3 (2017)
  • Droplet-based digital PCR system for detection of single-cell level of
           foodborne pathogens
    • Authors: Minjeong Jang; Soon Woo Jeong; Nam Ho Bae; Younseong Song; Tae Jae Lee; Moon Keun Lee; Seok Jae Lee; Kyoung G. Lee
      Abstract: Recently, foodborne pathogen is a common and distressing disease around world to cause a threat to life and economic damages and it is urgent to develop a tools to diagnosis of such pathogens in the early stage to prevent potential outbreak. Although conventional cell extraction and recovery of DNA from pathogen and PCR method have been widely used, the methods require complex steps, experts, and expensive chemicals and instruments to improve the PCR performance. Herein, we report a droplet-based polymerase chain reaction (ddPCR) system which allows identifying single-cell level of foodborne pathogens. E. coli O157:H7 and Salmonella cells were selected as model bacterial foodborne pathogens. The ddPCR system could be a useful platform for the quantitative detection of foodborne pathogens without any pretreatment process.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1410-x
  • Direct buffer composition of blood pre-process for nucleic acid based
    • Authors: YeJi Kim; Won-Nyoung Lee; Hyun Jin Yoo; Changyoon Baek; Junhong Min
      Abstract: Recently, a variety of methods, so called “direct buffer”, have been developed to utilize nucleic acid in the blood for the measurement of infectious bacteria and virus without any equipment in the field. In here, we first investigated the individual and combinatory effects of candidate chemicals which might be composed of the direct buffer on the PCR inhibition reduction of main compositions in whole blood. The long and short PEGs, Na2SO4 and GuSCN were selected as representative kosmotropic and chaotropic salts, respectively. MgCl2 were chosen as divalent cation source and NaOH was used to control blood pH. The effect of common non-ionic biological detergent was tested with Triton X-100 and SDS (Sodium Dodecyl sulfate) was chosen as anionic detergent. These results could provide a foundation for the development of sample preparation solution in nucleic acid based diagnostic field. As a result, the direct buffer developed in this study was able to detect viruses with a concentration of 102 pfu/100 μL of whole blood by a very simple method.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1401-y
  • Multi-electrochemical signal generation using metalloprotein based on
           selective surface modification
    • Authors: Yong-Ho Chung; Si-Youl Yoo; Jinho Yoon; Junhong Min; Jeong-Woo Choi
      Abstract: In this research, we demonstrate a simple method to generate a multi-peak in a single electrochemical signal for the application to bioelectronics devices. Myoglobin is used as a source of electrochemical signal, and its property of redox peak is controlled by the introduction of various chemical-linking materials. Based on controlled signals of myoglobin, multisignal having dual peak is achieved by the signal combination of six electrodes, which have different electrochemical signals by the modification of each electrode with different kinds of chemical linkers individually. For the device formation, selective immobilization process was performed by using reductive cleavage of pre-immobilized thiol group. As a result, the proposed concept shows simple and effective formation of a multi-signal in a single electrochemical signal as compared with other methods based on heterolayer and hybrid materials, and it can be directly applied to materialize practical bioelectronic devices such as a bio-logic gate or bio-computing system
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1409-3
  • Colorimetric Schirmer strip for tear glucose detection
    • Authors: Byoung-Hoon Kang; Moonseong Park; Ki-Hun Jeong
      Abstract: Human tears have attracted attention with easy-accessible indicators of healthcare monitoring. Recently self-monitoring of tear glucose in place of blood glucose has been developed with microfluidic paper-based analysis for a non-invasive diagnosis of diabetes. However, most previous works still have many limitations in unstable sample collection, low sensitivity, and insufficient sample volume. Here, we report a colorimetric Schirmer strip with preconcentration for tear glucose detection. A wax barrier on a paper strip enables tear collection of a fixed quantity as well as biocompatible incorporation of tear collection and detection by preventing flow reversal of reagents. Additional solvent was injected on a sampling site after tear collection, which accumulates the dispersed glucose into active site by capillary-driven flow for signal amplification. Glucose concentration was quantitatively detected at 0.1-2 mM by using a simple colorimetric method without any further image processing. The color changes in glucose concentration were clearly distinguishable between normal and diabetic patient-level. The colorimetric paper strip allows straightforward sensing platform for noninvasive diagnosis of diabetes and direct application of paper-based technology to the human body fluids.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1405-7
  • Effects of methamphetamine in the hippocampus of cynomolgus monkeys
           according to age
    • Authors: Mi Ran Choi; Sol Hee Bang; Yeung-Bae Jin; Youngjeon Lee; Han-Na Kim; Kyu-Tae Chang; Kyoung Hwa Jung; Sang-Rae Lee; Dai-Jin Kim
      Abstract: The aim of this study was to investigate differentially expressed genes (DEGs) and their functions in the hippocampi after methamphetamine (MA) administration in cynomolgus macaques according to age. Cynomolgus monkeys were divided by age as follows: control, 6–12 months (age 1, A1), 3–4 (A2) years, 7–9 (A3) years, and more than 11 years (A4). After MA was injected into the monkeys (2 mg/kg, intramuscular injection in each of A1, A2, A3 and A4), we performed large-scale transcriptome profiling in the hippocampus. Some genes associated with neuron differentiation, development, and transmission were validated with real-time RT-PCR. The expression patterns of validated genes were also investigated in the hippocampi of monkeys one year after a single injection of MA. The correlation of DEGs between A2 and A3 animals, who are similar to humans in early teens to early twenties, was higher than that of other groups, showing the possibility that biological mechanisms in the hippocampus of A2 and A3 are similarly affected by MA. Genes associated with neuron differentiation (FEZF1, GPR1N1, DLX1, and KCNIP2), neuron (projection) development (NGFR, CDKN1C, and VAX2), and synaptic transmission (SYT4, SYT1, GAD2, CORT, and NPTX2) were differentially expressed among control and MA-treated animals. In particular, the expression patterns of GPR1N1 among control and MA-treated animals following a single injection of MA were not altered even one year after single MA administration. Our results suggest that genes associated with neuron differentiation and synaptic transmission in the hippocampus of different maturation stages are differentially regulated by MA.
      PubDate: 2017-12-12
      DOI: 10.1007/s13206-017-1403-9
  • Signal enhancement in ATP bioluminescence to detect bacterial pathogens
           via heat treatment
    • Authors: Jinyeop Lee; Chanyong Park; Yonghee Kim; Sungsu Park
      Abstract: An ATP bioluminescence assay is frequently in the food industry as a commercially-available bacterial monitoring method because it is convenient and can monitor viable cells in food. However, such an assay suffers from low sensitivity and thus has limitations in monitoring highly pathogenic bacteria. In this study, we describe a method that improves the sensitivity by increasing the amount of ATP that is released from bacterial cells by subjecting the samples to heat treatment. When treated for 10 min at a temperature of 25 to 95°C in phosphate buffered saline (PBS) containing Escherichia coli O157:H7 or Salmonella enteritidis or Bacillus cereus at various concentrations, the relative luminescence unit (RLU) increased several times. Due to signal enhancement, the detection limit (LOD) of the ATP bioluminescence assay improved by about an order of magnitude in milk-containing microorganisms. Our results indicate that the simple heating step on the food samples before measurement is useful to improve the sensitivity of the ATP bioluminescence assay for bacterial detection.
      PubDate: 2017-12-06
      DOI: 10.1007/s13206-017-1404-8
  • Voltage regulation of retina neuron model with dynamic feedback for
           biological acquisition image
    • Authors: Ala’aDdin Al-Shidaifat; Sandeep Kumar; Hanjung Song
      Abstract: The investigation on biological activated imagers using standard CMOS processes has become continuous trend where silicon retina with central-plane image processing, small pixel sizes, large dynamic range and relatively low power consumption are required. This work proposes a voltage regulation of retina neuron model with dynamic feedback approach for biological acquisition image. The implementation of retina neuron circuit consists of conventional current-feedback event generator with the extension of proposed current mirror and dynamic feedback stage. The proposed neuron circuit achieves extremely high dynamic voltage range with respect to light intensity which help to detect biological acquisition image and could be beneficial for retinal prostheses. Moreover, individually modelling of photodiode using Verilog-A and device model is proposed for activation of current-feedback event generator. This modeling of photodiode permits to simple, compact and linear solution for pixel implementation. The proposed voltage controlled retina neuron circuit is implemented and fabricated using 0.18 μm Magnachip CMOS process. The spikes of output voltage are varied according to the inputs taken as control voltage and light intensity. As per the observation, read-out spikes of output voltage pulses provide more brightness level in the image pixels. The fabrication of proposed neuron circuit achieves less power consumption in nano-joule under dc supply of 3.3 V. The experimental result of output voltage is made good correlation with simulated one.
      PubDate: 2017-10-18
      DOI: 10.1007/s13206-017-1406-6
  • Gold nanoislands chip for Laser Desorption/Ionization (LDI) mass
    • Authors: Joo-Yoon Noh; Jo-Il Kim; Young Wook Chang; Jong-Min Park; Hyun-Woo Song; Min-Jung Kang; Jae-Chul Pyun
      Abstract: Gold nanoislands chip were developed as a solid matrix for the analysis of small molecules using laser desorption/ionization time-of-flight (LDIToF) mass spectrometry substituting the organic matrices which usually produce strong noise at low massto- charge ratio range (m/z<500). Gold nanoislands were simply prepared by (1) deposition of gold layer on highly P-doped silicon substrate followed by (2) heating. Physical properties of the gold nanoislands were analyzed by scanning electron microscope (SEM), atomic force microscopy (AFM) analysis to characterize the morphology, diameter, thickness, and surface density of the nanoislands which were comparatively analyzed in conjunction with LDI-ToF mass spectrometry. In this work, optimal size of the nanoislands were selected for LDI-ToF mass spectrometry, and gold nanoislands chip were applied to the quantitative and simultaneous detection of small analytes (amino acids) with various chemical and physical properties (polar, nonpolar, acidic, and basic).
      PubDate: 2017-09-08
      DOI: 10.1007/s13206-017-1310-0
  • Microfabricated cell culture system for the live cell observation of the
           multilayered proliferation of undifferentiated HT-29 cells
    • Authors: Young Lee; Je-Kyun Park
      Abstract: In this paper, we describe a microfabricated cell culture system (MCCS) for the microscopic live observation of cellular behavior in a reconstituted tissue-like microenvironment. The MCCS was used as a novel tool for tracking the proliferation of undifferentiated HT-29 cells. A wall structure of gelled extracellular matrix (ECM) protein was constructed inside the MCCS to establish a tissue-like microenvironment of the intestinal tissue. The ECM wall consisting of type I collagen retained the structural integrity during the gelation process and provided the adhesion surface for the HT-29 cells. We carried out the microscopic live observation of the proliferation of HT-29 cells seeded with various concentrations. The multilayered growth of HT-29 cells in the undifferentiated state was monitored without cell fixation, which was a necessary process in a conventional in vitro cell culture. The live tracking of cell culture also recorded the contraction of cell culture morphology during the initial period of cultivation. This approach is helpful for the establishment of an in vitro model for the live observation study of intestinal epithelial differentiation in an in vivo-like microenvironment.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1407-5
  • Prenatally diagnosed TTN mutation with repeated bilateral club foot by
           whole exome sequencing
    • Authors: So Hyun Shim; Ji Youn Kim; Se Ra Sung; Ji Eun Park; Yun Jeong Shin; Sung Han Shim; Dong Hyun Cha
      Abstract: With significant advances in genomic technologies over recent years, Next-generation sequencing (NGS) has become an important tool not only for gene discovery and research but also for clinical genetic diagnosis of rare disorders and single-gene-disorder analysis. Many hundreds of single-gene disorders have been described that can manifest prenatally and will comprise a large portion of undiagnosed, chromosomally normal cases but underlying cause of the abnormality still remains unknown for the large majority of cases with an abnormal ultrasound. However, there has been little experience and study in using trio whole exome sequencing (WES) for prenatal diagnosis. In this report the NGS method was applied for the prenatal genetic diagnosis of unidentified life-threatening phenotype in one family and confirmed the pattern of inheritance of titinopathy as well as two novel TTNgene variations. The report presented in these pages offers a unique and valuable perspective on relevance and feasibility of NGS in prenatal diagnosis field.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1309-6
  • Integrated analysis of miRNA and mRNA expression profiles in human
           endothelial cells exposed to fisetin
    • Authors: Seung Eun Lee; Hye Rim Park; Hong Duck Yun; Hyemi Kim; Young-Ho Jin; Jeong-Je Cho; Hyun-Jong Ahn; Cheung-Seog Park; Yong Seek Park
      Abstract: MicroRNAs (miRNAs) play vital regulatory roles in various biological processes including cell differentiation, proliferation and apoptosis. Several studies have examined the expression profiles of miRNAs in response to different drugs and chemicals and suggested that expression patterns were associated with the effects of drugs and chemicals. Fisetin, a polyphenol found in a wide range of plants, has been reported to exhibit numerous biological activities, such as antioxidant, anti-inflammatory, antiangiogenic, and antiproliferative properties. In the present study, we evaluated whether fisetin regulates miRNA and mRNA expression. Using microarray analysis, we measured global miRNA and mRNA expression in human endothelial cells treated with 10 μM of fisetin for 24 h. Using several bioinformatic systems, we determined the signatures of potential biological processes and signaling pathways such as cell migration, apoptotic signaling pathway, cell motility, and cardiovascular system development. Our findings provide insight into the molecular mechanisms of action of fisetin and can be used to improve therapeutic intervention in vascular diseases.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1308-7
  • Improvement of droplet speed and stability in electrowetting on dielectric
           devices by surface polishing
    • Authors: Hamidreza Shirinkami; Jiman Kim; Choonghee Lee; Hee Chan Kim; Honggu Chun
      Abstract: This paper reports on a technique that significantly improves the performance of open-plate electrowetting on dielectric (EWOD) devices by using chemical mechanical polishing (CMP) to achieve surface planarization. In EWOD devices, trenches are inevitably generated during fabrication, and these can have a considerable effect on droplet manipulation. Such artefacts lead to a local intensification in the electric field that not only hinders the droplet movement due to a partially increased adhesion force, but also limits the maximum applicable potential by increasing the likelihood of a dielectric breakdown. We conducted theoretical and experimental studies on the effect of trenches on the performance of both unpolished and polished EWOD devices. Various combinations of the droplet volume and electrode size were tested, and our proposed technique resulted in a notable improvement for all cases. For instance, when operating at 100 V, a 3.5 μL droplet could be translated at 70 mm/s on the polished EWOD device, which is twice as fast as that for its unpolished counterpart at the same voltage. Also, the maximum applicable potential resulting in a dielectric breakdown of the polished EWOD device was measured to be 27% higher than that of a conventional device.
      PubDate: 2017-08-25
      DOI: 10.1007/s13206-017-1408-4
  • Controlled production of monodisperse polycaprolactone microspheres using
           flow-focusing microfluidic device
    • Authors: Hyukjin J. Kwon; Suhyeon Kim; Sungwook Kim; Ju Hee Kim; Geunbae Lim
      Abstract: These days, biodegradable microsphere polymers have been attracting increasing interest as a cosmetic injectable filler. Particularly, polycaprolactone (PCL) microspheres are well known for their safety and long degradation time. However, these microspheres are usually produced by the conventional stirring method, which has an inherent drawback related to the size control of the microspheres; accurate size control is critical for the use of these microspheres as a filler. Here, we demonstrate the fabrication of monodisperse PCL microspheres in the size range of 26.6-161 μm using a flow-focusing microfluidic device. The acquired coefficient of variation of the solidified microspheres is approximately 4.5%; thus, these microspheres meet the requirement of being monodisperse. The study results show the feasibility of manufacturing PCL microspheres using a microfluidic device, and these microspheres have better morphological characteristics, thereby reducing pain and infection after their injection into the skin. Furthermore, the specific target size of the solidified microspheres is met. Therefore, undesired outcomes after microsphere injection through the dermis, such as phagocytosis and inflammatory reactions, are less likely to occur.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1306-9
  • miRNA-mediated expression switch of cell adhesion genes driven by
           microcirculation in chip
    • Authors: Timur R Samatov; Vladimir V Galatenko; Nadezhda V Senyavina; Alexey V Galatenko; Maxim Yu Shkurnikov; Svetlana A Tonevitskaya; Dmitry A Sakharov; Uwe Marx; Hermann Ehrlich; Udo Schumacher; Alexander G Tonevitsky
      Abstract: Changes in cell adhesion molecule (CAM) expression and miRNAs regulating them are known to be involved in malignant progression in colon cancer. We investigated expression profiles of CAM genes and non-coding RNAs in CaCo2 colon cancer cells in static culture and under dynamic flow conditions perfused in microfluidic chip emulating physiological microenvironment. We incubated monolayers of CaCo2 cells in Transwell® units either under static conditions or under flow in a microfluidic chip. We identified 7 up-regulated CAM genes (CD44, CDH7, CEACAM5, CEACAM6, CYR61, L1CAM and VCAN), 7 down-regulated genes (COL12A1, FGA, FGB, FGG, GJA1, ITGA5 and LAMA1) and 69 miRNAs targeting them under the influence of microcirculation. The revealed network comprised CAM genes known to interact with each other and 13 miRNAs simultaneously regulating more than one of them. The discovered regulatory network comprising CAM genes and miRNAs is likely involved in normal functioning of intestine epithelium as well as in cancer progression.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1305-x
  • Three-dimensional in vitro gut model on a villi-shaped collagen scaffold
    • Authors: Banya Yi; Kyu Young Shim; Sang Keun Ha; Jeonghun Han; Hong-Hoa Hoang; Inwook Choi; Sungsu Park; Jong Hwan Sung
      Abstract: A three-dimensional (3D) tissue architecture provides essential cellular environmental cues for cell differentiation. We previously reported that Caco-2 human intestinal epithelial cells grown on a 3D villi-shaped scaffold composed of collagen created a tissue structure resembling the human intestinal villi. Here, we compared the absorptive and metabolic properties of Caco-2 cells cultured on the 3D villi with those in 2D monolayer. Cell growth was higher in the 3D villi model compared with the 2D monolayer, probably owing to an increase in the available surface area. Barrier functions were more in vivo-like in the 3D villi model in association with reduced expression of tight junction and transporter proteins. The specific activities of the metabolic enzyme and intestinal epithelial differentiation marker, alkaline phosphatase, were improved in the 3D villi model, whereas aminopeptidase activity was comparable in the two models. These results suggest that the 3D villi scaffold induces physiological changes in Caco-2 cells, related to the absorption and metabolism of drugs. This 3D villi model may serve as an alternative and improved in vitro gut model for studying drug metabolism and transport.
      PubDate: 2017-06-02
      DOI: 10.1007/s13206-017-1307-8
  • PDMS microchannel surface modification with Teflon for algal lipid
    • Authors: Jae Woo Park; Sangcheol Na; Myeongwoo Kang; Sang Jun Sim; Noo Li Jeon
      Abstract: This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs without background signal from absorbed dye molecules on PDMS microchannel. We confirmed that contact angle of Teflon-coated PDMS (116.4°) is higher than that of unmodified PDMS (106.1°) and thus more hydrophobic. Furthermore, Teflon-coated PDMS surface had ~80% of oxygen transfer rate compared to that of native PDMS and good transparency in all visible light regions. Based on these characteristics, we successfully validated the visualization and quantification of intracellular lipid droplets in microalgae C. reinhardtii using BODIPY. We believe that our new method will expand microfluidic applications on characterization of biological lipid with fluorescence probes and biochemical markers.
      PubDate: 2017-03-03
      DOI: 10.1007/s13206-017-1302-0
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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Fax: +00 44 (0)131 4513327
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