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BIOLOGY (1470 journals)                  1 2 3 4 5 6 7 8 | Last

Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 21)
Achievements in the Life Sciences     Open Access   (Followers: 5)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 24)
Acta Biologica Colombiana     Open Access   (Followers: 7)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 4)
Acta Biologica Sibirica     Open Access  
Acta Biomaterialia     Hybrid Journal   (Followers: 27)
Acta Biotheoretica     Hybrid Journal   (Followers: 4)
Acta Chiropterologica     Full-text available via subscription   (Followers: 6)
acta ethologica     Hybrid Journal   (Followers: 4)
Acta Limnologica Brasiliensia     Open Access   (Followers: 3)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales : The Journal of Silesian Museum in Opava     Open Access  
Acta Neurobiologiae Experimentalis     Open Access  
Acta Parasitologica     Hybrid Journal   (Followers: 10)
Acta Scientiarum. Biological Sciences     Open Access   (Followers: 2)
Acta Scientifica Naturalis     Open Access   (Followers: 3)
Actualidades Biológicas     Open Access   (Followers: 1)
Advanced Health Care Technologies     Open Access   (Followers: 4)
Advanced Studies in Biology     Open Access  
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 2)
Advances in Bioinformatics     Open Access   (Followers: 17)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4)
Advances in Biosensors and Bioelectronics     Open Access   (Followers: 7)
Advances in Cell Biology     Open Access   (Followers: 25)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Ecological Research     Full-text available via subscription   (Followers: 42)
Advances in Environmental Sciences - International Journal of the Bioflux Society     Open Access   (Followers: 17)
Advances in Enzyme Research     Open Access   (Followers: 9)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Genome Biology     Full-text available via subscription   (Followers: 8)
Advances in High Energy Physics     Open Access   (Followers: 18)
Advances in Human Biology     Open Access   (Followers: 3)
Advances in Life Science and Technology     Open Access   (Followers: 16)
Advances in Life Sciences     Open Access   (Followers: 6)
Advances in Marine Biology     Full-text available via subscription   (Followers: 15)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 21)
Advances in Organ Biology     Full-text available via subscription   (Followers: 1)
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3)
Advances in Regenerative Biology     Open Access   (Followers: 1)
Advances in Space Biology and Medicine     Full-text available via subscription   (Followers: 5)
Advances in Structural Biology     Full-text available via subscription   (Followers: 5)
Advances in Virus Research     Full-text available via subscription   (Followers: 5)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 6)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 1)
Ageing Research Reviews     Hybrid Journal   (Followers: 10)
Aging Cell     Open Access   (Followers: 12)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat     Open Access  
AJP Cell Physiology     Full-text available via subscription   (Followers: 14)
AJP Endocrinology and Metabolism     Full-text available via subscription   (Followers: 23)
AJP Lung Cellular and Molecular Physiology     Full-text available via subscription   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
Alces : A Journal Devoted to the Biology and Management of Moose     Open Access  
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Biology Teacher     Full-text available via subscription   (Followers: 14)
American Fern Journal     Full-text available via subscription   (Followers: 1)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 8)
American Journal of Bioethics     Hybrid Journal   (Followers: 10)
American Journal of Human Biology     Hybrid Journal   (Followers: 13)
American Journal of Medical and Biological Research     Open Access   (Followers: 8)
American Journal of Plant Sciences     Open Access   (Followers: 18)
American Journal of Primatology     Hybrid Journal   (Followers: 14)
American Malacological Bulletin     Full-text available via subscription   (Followers: 3)
American Naturalist     Full-text available via subscription   (Followers: 70)
Amphibia-Reptilia     Hybrid Journal   (Followers: 6)
Anaerobe     Hybrid Journal   (Followers: 4)
Analytical Methods     Full-text available via subscription   (Followers: 10)
Anatomical Science International     Hybrid Journal   (Followers: 2)
Animal Cells and Systems     Hybrid Journal   (Followers: 4)
Annales de Limnologie - International Journal of Limnology     Hybrid Journal   (Followers: 1)
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3)
Annales UMCS, Biologia     Open Access   (Followers: 1)
Annals of Applied Biology     Hybrid Journal   (Followers: 7)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Human Biology     Hybrid Journal   (Followers: 5)
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 15)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 23)
Annual Review of Cancer Biology     Full-text available via subscription   (Followers: 1)
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 37)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 23)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 10)
Anthropological Review     Open Access   (Followers: 23)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antibiotics     Open Access   (Followers: 9)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 8)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 8)
Applied Bionics and Biomechanics     Open Access   (Followers: 8)
Applied Vegetation Science     Full-text available via subscription   (Followers: 10)
Aquaculture Environment Interactions     Open Access   (Followers: 2)
Aquaculture International     Hybrid Journal   (Followers: 22)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 6)
Aquatic Biology     Open Access   (Followers: 5)
Aquatic Ecology     Hybrid Journal   (Followers: 33)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 14)
Aquatic Science and Technology     Open Access   (Followers: 3)
Aquatic Toxicology     Hybrid Journal   (Followers: 21)
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 3)
Archives of Biological Sciences     Open Access  
Archives of Microbiology     Hybrid Journal   (Followers: 8)
Archives of Natural History     Hybrid Journal   (Followers: 6)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 5)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arid Ecosystems     Hybrid Journal   (Followers: 2)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 2)
Arthropods     Open Access   (Followers: 1)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 3)
Artificial Photosynthesis     Open Access   (Followers: 1)
Asian Bioethics Review     Full-text available via subscription   (Followers: 3)
Asian Journal of Biodiversity     Open Access   (Followers: 4)
Asian Journal of Biological Sciences     Open Access   (Followers: 3)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Developmental Biology     Open Access   (Followers: 2)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 3)
Asian Journal of Nematology     Open Access   (Followers: 4)
Asian Journal of Poultry Science     Open Access   (Followers: 3)
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 6)
Autophagy     Hybrid Journal   (Followers: 2)
Avian Biology Research     Full-text available via subscription   (Followers: 4)
Avian Conservation and Ecology     Open Access   (Followers: 11)
Bacteriology Journal     Open Access   (Followers: 1)
Bacteriophage     Full-text available via subscription   (Followers: 3)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access   (Followers: 1)
Berita Biologi     Open Access   (Followers: 1)
Between the Species     Open Access   (Followers: 1)
Bio Tribune Magazine     Hybrid Journal  
BIO Web of Conferences     Open Access  
BIO-Complexity     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bioanalytical Reviews     Hybrid Journal   (Followers: 2)
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 6)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 15)
Biochimie     Hybrid Journal   (Followers: 7)
BioControl     Hybrid Journal   (Followers: 5)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal  
BioDiscovery     Open Access   (Followers: 2)
Biodiversitas : Journal of Biological Diversity     Open Access  
Biodiversity : Research and Conservation     Open Access   (Followers: 26)
Biodiversity Data Journal     Open Access   (Followers: 3)
Biodiversity Informatics     Open Access   (Followers: 1)
Biodiversity Information Science and Standards     Open Access  
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro     Open Access  
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 2)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 2)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
Bioethics     Hybrid Journal   (Followers: 14)
BioéthiqueOnline     Open Access  
Biofabrication     Hybrid Journal   (Followers: 5)
Biofilms     Full-text available via subscription   (Followers: 1)
Biogeosciences (BG)     Open Access   (Followers: 10)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 2)
Bioinformatics     Hybrid Journal   (Followers: 291)
Bioinformatics and Biology Insights     Open Access   (Followers: 11)
Bioinspiration & Biomimetics     Hybrid Journal   (Followers: 7)
Biointerphases     Open Access   (Followers: 1)
Biojournal of Science and Technology     Open Access  
Biologia     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 6)
Biological Control     Hybrid Journal   (Followers: 4)
Biological Invasions     Hybrid Journal   (Followers: 18)
Biological Journal of the Linnean Society     Hybrid Journal   (Followers: 18)
Biological Letters     Open Access   (Followers: 5)
Biological Procedures Online     Open Access  
Biological Psychiatry     Hybrid Journal   (Followers: 45)
Biological Psychology     Hybrid Journal   (Followers: 7)
Biological Research     Open Access  
Biological Rhythm Research     Hybrid Journal   (Followers: 2)
Biological Theory     Hybrid Journal   (Followers: 2)
Biological Trace Element Research     Hybrid Journal  
Biologicals     Full-text available via subscription   (Followers: 9)
Biologics: Targets & Therapy     Open Access   (Followers: 1)
Biologie Aujourd'hui     Full-text available via subscription   (Followers: 1)
Biologie in Unserer Zeit (Biuz)     Hybrid Journal   (Followers: 40)
Biologija     Open Access  
Biology     Open Access   (Followers: 3)
Biology and Philosophy     Hybrid Journal   (Followers: 19)

        1 2 3 4 5 6 7 8 | Last

Journal Cover Aquaculture Reports
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 2352-5134
   Published by Elsevier Homepage  [3177 journals]
  • Developing a national spat collection program for pearl oysters in the
           Fiji Islands supporting pearl industry development and livelihoods

    • Authors: Pranesh Kishore; Garry Bingnald Vuibeqa; Paul C. Southgate
      Pages: 46 - 52
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Pranesh Kishore, Garry Bingnald Vuibeqa, Paul C. Southgate
      Cultured pearl farming in Fiji relies on wild spat collection to supply the oysters used for pearl production. This supply can be inconsistent and a research program was implemented to determine recruitment of pearl oysters to spat collectors at sites throughout Fiji as a basis for developing a national spat collection program to improve reliability of oyster supply to the industry. Twenty-nine sites across Fiji were used in this study. Spat collectors consisted of a 100 m longline from which 310 individual spat collectors were suspended. Spat collectors were deployed for a period of 10–15 months when the number of pearl oyster (Pinctada margaritifera and Pteria penguin) spat attached to each collector was counted and shell size recorded. A total of 5478 P. margaritifera juveniles were collected from all sites with the highest number of recruits (693) and the highest number of recruits per collector (2.10 ± 0.17) occurring at Nacobau (Vanua Levu). The largest mean dorso-ventral measurement (DVM) of P. margaritifera at any site was 8.61 ± 0.30 cm while the smallest was 4.26 ± 0.13 cm. Some sites did not record any P. margaritifera recruitment during the study and these were generally sites with relatively turbid water. A total of 4224 Pt. penguin were collected from all sites, with the highest number of recruits (495) recorded from Namarai (Viti Levu). The mean DVM of Pt. penguin ranged from 7.53 cm to 13.62 cm across sites. Results indicate that Pt. penguin have greater tolerance of more turbid inshore sites than P. margaritifera based on greater levels of recruitment at these sites. Results identified sites supporting high levels of pearl oyster recruitment as a basis for an ongoing national spat collection program, and support better targeting of spat collection activities that maximise oyster supply to the Fijian pearl industry. The national spat collection program will generate significant livelihood benefits across Fiji and support continued expansion of the Fijian cultured pearl industry.

      PubDate: 2018-01-05T15:37:32Z
      DOI: 10.1016/j.aqrep.2017.12.004
      Issue No: Vol. 9 (2018)
  • Effects of host gut-derived probiotic bacteria on gut morphology,
           microbiota composition and volatile short chain fatty acids production of
           Malaysian Mahseer Tor tambroides

    • Authors: Md. Asaduzzaman; Shumpei Iehata; Sumi Akter; Md. Abdul Kader; Subrata Kumar Ghosh; M. Nurul Absar Khan; Ambok Bolong Abol-Munafi
      Pages: 53 - 61
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Md. Asaduzzaman, Shumpei Iehata, Sumi Akter, Md. Abdul Kader, Subrata Kumar Ghosh, M. Nurul Absar Khan, Ambok Bolong Abol-Munafi
      Three host-associated probiotics (Bacillus sp. AHG22, Alcaligenes sp. AFG22, and Shewanella sp. AFG21) were isolated from the gastrointestinal tract of Tor tambroides, and their effects were evaluated on gut morphology, microbiota composition and volatile short chain fatty acids (VSCFAs) production of the same species. A control diet (40% crude protein and 10% lipid) was formulated, and three different probiotic supplemented diets were prepared by immersing the control diet in each host-derived isolated probiotic, suspended in sterile phosphate buffered saline (PBS), to achieve concentration at 1.0 × 108 CFU g−1 feed. Triplicate groups of T. tambroides juveniles (1.39 ± 0.06 g) were stocked in twelve glass aquaria (100 L capacity) with stocking density of 20 individuals per aquarium. The feed was applied twice daily at 3.0% of the body weight per day for 90 days. The intake of probiotics drastically modified the gut microbiota composition. The average number of OTUs, Shannon index and Margalef species richness were significantly higher in host-associated probiotic treatments compared to the control. A significant increase of lipolytic, proteolytic and cellulolytic bacterial number were observed in the gastrointestinal tracts of T. tambroides fed the diets supplemented with Alcaligenes sp. AFG22 compared to the control. Villus length, villus width and villus area were significantly higher in T. tambroides juveniles fed the diet supplemented with Alcaligenes sp. AFG22. Acetate and butyrate were detected as main VSCFA production in the gastrointestinal tract of T. tambroides. Acetate and total VSCFAs production in Alcaligenes sp. AFG22 supplemented treatment was significantly higher than control. These results indicate that host-derived probiotics, especially Alcaligenes sp. has a significant potential as an important probiotic to enhance the nutrients utilization and metabolism through increasing gut surface area and VSCFAs production, and adjusting gut microbiota balance of T. tambroides juveniles.

      PubDate: 2018-01-05T15:37:32Z
      DOI: 10.1016/j.aqrep.2017.12.003
      Issue No: Vol. 9 (2018)
  • Impact of spat shell colour selection in hatchery-produced Pinctada
           margaritifera on cultured pearl colour

    • Authors: Chin-Long Ky; Manaari Sham Koua; Gilles Le Moullac
      Pages: 62 - 67
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Chin-Long Ky, Manaari Sham Koua, Gilles Le Moullac
      Beaded cultured pearl farming is a lengthy aquaculture process, particularly when the pearl oysters are produced through a hatchery propagation system, and includes the key steps of artificial breeding, larval and spat rearing before graft operations can take place. Within its genus, Pinctada margaritifera has the ability to produce the widest range of pearl colours, thanks to the donor colour polymorphism of the inner shell, which is mainly responsible for colour transmission. As hatchery spat production in P. margaritifera leads to several colour phenotypes (at 3 months old), the aim of this study was to determine whether a relation exists between the colour of the donors as spat and the final pearl colour. In the experiment, which took place over a four-year period, earlier spat colour selection was applied to two hatchery-produced P. margaritifera families. The spat were traced and then used as donors at the adult stage. A total of 1100 experimental grafts were made, using originally grey, green, red and yellow spat phenotypes as donors. The results showed that all spat colour phenotypes mainly produced pearls in the moderately dark (78.4%) and grey colour (56.7%) classes. Differences in darkness level were produced by red and yellow spat, whose pearls were about twice as pale as those from the grey and green phenotypes. Concerning the pearl colour categories, the results showed that the attractive green/blue pearls were obtained twice as often when using grey and green spat phenotypes and that aubergine/peacock pearls were obtained four times more often by using the red and yellow spat phenotypes. This preliminary study suggests that earlier phenotypic colour selection could be applied in P. margaritifera spat as a useful indicator in both pearl production cycles and family selection for donor oyster lines of specific colour propagation.

      PubDate: 2018-01-16T03:36:07Z
      DOI: 10.1016/j.aqrep.2017.12.002
      Issue No: Vol. 9 (2018)
  • Valve-gaping behavior of raft-cultivated mussels in the Ría de
           Arousa, Spain

    • Authors: Luc A. Comeau; Jose M.F. Babarro; Angeles Longa; Xose A. Padin
      Pages: 68 - 73
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Luc A. Comeau, Jose M.F. Babarro, Angeles Longa, Xose A. Padin
      We describe the valve-opening behavior of raft-cultivated mussels (Mytilus galloprovincialis) in the Ría de Arousa (Arousa estuary), Spain. Eight rope-grown mussels [mean ± standard error (SEM), shell length 61.6 ± 2.1 mm] were connected to a non-invasive valvometry apparatus that monitored (one measurement min−1) the magnitude of valve openness systematically over a 10 day period. It was found that valves were open 97.5 ± 1.3% percent of the time. Valve closures were not synchronized among the eight monitored mussels, suggesting that feeding cessation was physiologically-regulated rather than environmentally-mediated. The opening amplitudes that were most frequently observed were in the range of 60–90%, indicating that, when open, valves are usually opened relatively close to their maximum possible extent. The majority (7/8) of mussels displayed a circadian rhythm (τ = 24.0 h) in valve opening amplitude. They tended to exhibit maximum valve opening during nighttime and minimum opening during daytime. It is possible that the light:dark cycle represents an environmental zeitgeber entraining an endogenous gaping rhythm in this bivalve.

      PubDate: 2018-01-16T03:36:07Z
      DOI: 10.1016/j.aqrep.2017.12.005
      Issue No: Vol. 9 (2018)
  • Production of periphyton to enhance yield in polyculture ponds with carps
           and small indigenous species

    • Authors: Sabita Jha; Sunila Rai; Madhav Shrestha; James S. Diana; Ram Bhajan Mandal; Hillary Egna
      Pages: 74 - 81
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Sabita Jha, Sunila Rai, Madhav Shrestha, James S. Diana, Ram Bhajan Mandal, Hillary Egna
      Although carp polyculture is well established throughout southern Asia, its overall efficiency in providing sufficient nutrients and financial profit remains variable. Site-specific adjustments are needed to improve efficiencies of polyculture under local circumstances. We evaluated variations of carp polyculture systems in two separate trials: one on a research station (on-station), and one in farmers’ ponds (on-farm). The on-station experiment included four treatments: TF (carp + 100% feed), TFS (carp + SIS (small indigenous species) +100% feed), TFSP (carp + SIS + 50% feed + bamboo substrate) and TSP (carp + SIS+ bamboo substrate with no feed), each done with three replicates. Silver carp (Hypophthalmichthys molitrix), bighead carp (Aristichthys nobilis), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), rohu (Labeo rohita), and mrigal (Cirrhinus mrigala) were stocked at a ratio of 4:1:4:3:5:5 and a rate of 15,000 fish/ha. Additionally, 2 SIS, dedhuwa (Esomus danricus) and pothi (Puntius sophore), were stocked at 1:1 and a combined density of 50,000 fish/ha. Carps were fed daily at 5% of body weight (BW) for 60 days, then 2% BW for 150 days, using a supplemental feed composed of dough (mustard oil cake and rice bran (1:1)), or using grass (for grass carp). Total carp yield and FCR were highest in TFSP ponds. Gross margin was also higher in treatments enhanced with periphyton (TFSP and TSP). Overall, TFSP was determined the best on-station result, based on total production of fish and profit. The two treatments with the highest net fish yield, TF and TFSP, were introduced to 37 women farmers in Chitwan and Nawalparasi districts for on-farm trials. After 8 months of culture, total fish weight and gross margin were 24.0% and 51.2% higher, respectively, in TFSP ponds than in TF ponds. Reduced feed application with increased periphyton enhancement dramatically improved profit while maintaining fish yields similar to those of traditional polyculture systems with full feeding.

      PubDate: 2018-02-26T06:02:02Z
      DOI: 10.1016/j.aqrep.2018.01.001
      Issue No: Vol. 9 (2018)
  • Development of immune functionality in larval and juvenile crimson snapper
           Lutjanus erythropterus (Bloch 1790)

    • Authors: Ke Cui; Zhilu Fu; Dachuan Cheng; Qibin Yang; Zhenhua Ma; Jian G. Qin; Jing Hu
      Pages: 1 - 7
      Abstract: Publication date: May 2018
      Source:Aquaculture Reports, Volume 10
      Author(s): Ke Cui, Zhilu Fu, Dachuan Cheng, Qibin Yang, Zhenhua Ma, Jian G. Qin, Jing Hu
      Ontogenetic development of the immune system in crimson snapper (Lutjanus erythropterus Bloch 1790) larvae was histologically and enzymatically studied from hatch to 36 days post-hatch (DPH). Primitive hepatopancreas appeared on 2 DPH and renal tubules started hematopoiesis on 4 DPH. The spleen anlage appeared on 6 DPH and the thymus formed on 14 DPH. Total activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and sodium-potassium adenosine triphosphatase (Na+ K+-ATPase) gradually increased after hatch, and showed a sharp increase after 29 DPH during the transitional feeding period from Artemia to inert feed. The specific activities of SOD, CAT, and GPX showed a trend of sharp increase and reached the maximum level on 4 DPH when exogenous feeding started, except for Na+ K+-ATPase where the peak occurred on10 DPH. The specific activities of these five enzymes reached the peak during the food transition from rotifers to Artemia, but the total activity of enzymes showed an increasing trend as fish grew. The present study provides new knowledge of the development of functional enzymes relevant to fish larvae immunity, sheds light on the understanding of the change of larval health, and improves hatchery management of crimson snapper.

      PubDate: 2018-03-08T06:33:35Z
      DOI: 10.1016/j.aqrep.2018.02.001
      Issue No: Vol. 10 (2018)
  • Metabolic and immune effects of orange-spotted grouper, Epinephelus
           coioides induced by dietary arginine

    • Authors: Fenglu Han; Shuyan Chi; Beiping Tan; Xiaohui Dong; Qihui Yang; Hongyu Liu; Shuang Zhang; Di Liu; Yuanfa He
      Pages: 8 - 16
      Abstract: Publication date: May 2018
      Source:Aquaculture Reports, Volume 10
      Author(s): Fenglu Han, Shuyan Chi, Beiping Tan, Xiaohui Dong, Qihui Yang, Hongyu Liu, Shuang Zhang, Di Liu, Yuanfa He
      Arginine (Arg) as an important substrate for protein metabolism and endocrine influences on fish health directly. To evaluate the effect of dietary Arg on these endocrine and immunity of juvenile orange-spotted grouper (Epinephelus coioides), an experiment adding Arg with 0.00, 0.30, 0.60, 0.90, 1.20, 1.50, and 1.80% in seven diets was conducted. Dietary Arg levels reached 2.13 (Diet 1), 2.42 (Diet 2), 2.71 (Diet 3), 2.95 (Diet 4), 3.20 (Diet 5), 3.48 (Diet 6) and 3.74% (Diet 7), respectively. Each treatment was randomly assigned to triplicate groups of 30 fish (7.52 ± 0.02 g) each tank. The present results indicated that different Arg levels of diets were no significant effects on the fish survival rate of each groups (P > 0.05) after 8 weeks feeding experiment. The highest weight gain and specific growth rate were found in Diet 4, and significantly higher than the other groups (P < 0.05). Hepatic arginase activities were significantly higher in fish fed Diet 6 and Diet 7 compared to the activities of the fish fed first three Diets (P < 0.05). Hepatic ornithine decarboxylase activities of fish fed Diet 1 and Diet 2 were significantly decreased compared to the fish fed Diet 4–Diet 7 (P < 0.05). With ascending dietary Arg, the serum Insulin (INS) and insulin-like growth factor I (IGF-I) levels significantly increased. Gene expression of tissue toll-like receptors 22 in the three tissues were found first up-regulated and then down-regulated with the Arg increasing. Gene expression of major histocompatibility complex II in liver and kidney significantly increased in fish fed Diet 4 and Diet 6, and decreased thereafter. However, gene expression of hepcidin decreased with dietary Arg increased. The present study showed alterations in serum INS and IGF-I levels in response to Arg-induced growth activation. Additionally, the results indicate that Arg has beneficial effects on regulating mRNA expression of immune-associated genes and improving humoral and cellular immunity of fish.

      PubDate: 2018-03-20T06:45:51Z
      DOI: 10.1016/j.aqrep.2018.03.002
      Issue No: Vol. 10 (2018)
  • Effects of dietary protein to energy ratios on growth performance of
           yellowfoot limpet (Cellana sandwicensis Pease, 1861)

    • Authors: Anthony Mau; Rajesh Jha
      Pages: 17 - 22
      Abstract: Publication date: May 2018
      Source:Aquaculture Reports, Volume 10
      Author(s): Anthony Mau, Rajesh Jha
      The aquaculture of yellowfoot limpets (Cellana sandwicensis) is a prospect industry in research and development. The effects of dietary protein to energy (PE) ratio on growth performance were evaluated for 180 days in a flow-through system. Replicate animals (5.9 ± 1.72 g and 33.9 ± 2.13 mm) were stocked randomly on individual plates, and four paste diets containing PE ratios ranging from 87.2 to 102.9 mg/kcal were offered once daily (1600 h). A significant increase in daily feed intake (P < 0.05) was observed to coincide with seasonal decrease in air temperature. Although dietary treatment had no significant effect on overall growth performance (P > 0.05), average daily gain (ADG) and feed conversion efficiency (FCE) improved both linearly and quadratically (ADG P = 0.03, P = 0.08; FCE P = 0.05, P = 0.04, respectively). These results indicate potential seasonal growth patterns, which are controlled by environmental cues (i.e. temperature, feed availability, etc.) and must be considered in future trials. Limpets offered higher PE ratio diets did not compensate for lower energy levels with increased feed intake, and specific growth rate increased up to 0.20% BW/d as the dietary PE ratio decreased. A PE ratio of 87.2 mg/kcal produced the best tissue growth and can be recommended as a suitable formulated diet for limpet production.

      PubDate: 2018-03-20T06:45:51Z
      DOI: 10.1016/j.aqrep.2018.03.001
      Issue No: Vol. 10 (2018)
  • Modulation of growth performance, immunological responses and disease
           resistance of juvenile Nile tilapia (Oreochromis niloticus) (Linnaeus,
           1758) by supplementing dietary inosine monophosphate

    • Authors: Md. Abdul Kader; Mahbuba Bulbul; Ambok Bolong Abol-Munafi; Md. Asaduzzaman; Sohel Mian; Noordiyana Bt. Mat Noordin; Md. Eaqub Ali; Md. Sakhawat Hossain; Shunsuke Koshio
      Pages: 23 - 31
      Abstract: Publication date: May 2018
      Source:Aquaculture Reports, Volume 10
      Author(s): Md. Abdul Kader, Mahbuba Bulbul, Ambok Bolong Abol-Munafi, Md. Asaduzzaman, Sohel Mian, Noordiyana Bt. Mat Noordin, Md. Eaqub Ali, Md. Sakhawat Hossain, Shunsuke Koshio
      This study was investigated to examine supplemental effects of dietary inosine monophosphate (IMP) on growth performance, feed utilization, biochemical, hematological and immunological parameters of juvenile Nile tilapia Oreochromis niloticus. Disease resistance to experimental infection with Streptococcus agalactiae was also assessed. A semi-purified basal diet was supplemented with 0 (IMP0, Control), 1 (IMP1), 2 (IMP2), 4 (IMP4) and 8 (IMP8) g purified IMP kg−1 diet to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (0.59 g) for 60 days. The results indicated that supplementation of IMP significantly (P < 0.05) improved growth performances of fish. The highest final weight was found in IMP2 followed by IMP8, IMP4 and IMP1. Feed utilization parameters were also positively influenced by dietary IMP supplementation and IMP2 supplemented group showed improved values. Feed intake increased with dietary IMP supplementations but not at a significant level (P > 0.05). Among whole body proximate composition and somatic parameters, condition factor was significantly influenced by dietary supplementation of IMP. A wide variation in hematological parameters were observed and dietary supplementation increased the hematocrit content (P < 0.05) and red blood cells (P > 0.05). Total serum protein (TSP), lysozyme activity (LA), superoxide dismutase activity (SOD) and bactericidal activity (BA) tended to increase with the supplementation of dietary IMP. TSP and SOD were significantly improved with ≥4 g kg−1 supplementation, while LA with 8 g kg−1 and BA with ≥1 g kg−1 supplementations. IMP supplemented groups showed higher (P > 0.05) cumulative survival compared to that of supplementation free control group. IMP supplemented diet groups also showed significantly higher BA in the post challenge test. Based on the overall performances, the results of the current study indicated that the inclusion of IMP in Nile tilapia diet can improve growth performance, feed utilization, haematological and immunological parameters; and disease resistance of juvenile Nile tilapia.

      PubDate: 2018-04-15T07:24:27Z
      DOI: 10.1016/j.aqrep.2018.03.003
      Issue No: Vol. 10 (2018)
  • Temperature and diet effect on the pepsin enzyme activities, digestive
           somatic index and relative gut length of Malabar blood snapper (Lutjanus
           malabaricus Bloch & Schneider, 1801)

    • Authors: Sabuj Kanti Mazumder; Simon Kumar Das; Saleh M. Rahim; Mazlan Abd Ghaffar
      Pages: 1 - 9
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Sabuj Kanti Mazumder, Simon Kumar Das, Saleh M. Rahim, Mazlan Abd Ghaffar
      An integrated experiment was performed on juvenile Malabar blood snapper (Lutjanus malabaricus) to investigate the effect of temperature and diet in their pepsin activities in relation with digestive somatic index (I DS) and relative gut length (RGL). One hundred twenty L. malabaricus juvenile (13–15cm) were equally distributed among four exposed temperature treatments (22, 26, 30 and 34°C) representing their seasonal range and to account for end of century predicted temperatures, and two diets as commercial pellet and natural shrimp. After 7days of acclimation period fish were reared for 30days in twenty four 400l glass aquaria at a stocking density of 5 fish tank−1. All treatments were three replications. The result showed that, I DS and RGL gradually decreased with increasing temperature up to 30°C and again increase at 34°C. And the values were also higher in pellet feeding fish than shrimp feeding fish at all the temperatures. Alternatively, in pepsin activity, an increased activity was seen between 26°C to 30°C and this activity was significantly higher than the 22°C and 34°C (P< 0.05). In general, highest pepsin activity was observed among fish which fed on a natural shrimp diet reared at temperature 30°C (5.47±1.60Umgprotein−1), followed by those at 26, 34 and 22°C (P< 0.05) at both diet however, no mortalities were observed. These results could be used as a basis for selecting a suitable diet for maximizing the growth and sustainable aquaculture coping with global warming.

      PubDate: 2017-12-11T10:11:12Z
      DOI: 10.1016/j.aqrep.2017.11.003
      Issue No: Vol. 9 (2017)
  • Novel technique to identify large river host fish for freshwater mussel
           propagation and conservation

    • Authors: Michael A. Hart; Wendell R. Haag; Robert Bringolf; James A. Stoeckel
      Pages: 10 - 17
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Michael A. Hart, Wendell R. Haag, Robert Bringolf, James A. Stoeckel
      Skipjack Herring (Alosa chrysochloris) has long been proposed as the sole host for Reginaia ebenus (Ebonyshell) and Elliptio crassidens (Elephantear), but these relationships were unconfirmed because of difficulties with maintaining this fish species in captivity. We confirmed the suitability of Skipjack Herring as host for both mussel species, and we also showed that Alabama Shad (Alosa alabamae) is an additional suitable host for E. crassidens; both fish species produced large numbers of juvenile mussels. No other fish species tested (n=12) were suitable hosts for either mussel species. Our results, combined with results from other studies, suggest these mussel species are specialists on genus Alosa. Traditional methods for host identification were problematic for herrings because of their sensitivity to handling and the large volumes of water required to maintain them in captivity. In addition to traditional methods, we confirmed the suitability of these fishes as hosts using a novel technique in which fish gills infected with glochidia were excised from sacrificed fishes and held in recirculating holding tanks with flow until metamorphosis was complete. Completion of metamorphosis on excised gills required glochidia spend at least 11–17 d encapsulated on live fishes before gill excision. This technique may be useful for other large or sensitive fishes that do not lend themselves well to traditional methods for host identification. Confirmation of Alosa spp. as primary hosts for R. ebenus and E. crassidens supports the idea that dams and other river modifications that disrupt migrations of these fishes are key factors in the range restrictions of these mussel species.

      PubDate: 2017-12-11T10:11:12Z
      DOI: 10.1016/j.aqrep.2017.11.002
      Issue No: Vol. 9 (2017)
  • Survival and growth of fish (Lates calcarifer) under integrated
           mangrove-aquaculture and open-aquaculture systems

    • Authors: Shanmugaarasu Venkatachalam; Kathiresan Kandasamy; Ilanchelian Krishnamoorthy; Rajendran Narayanasamy
      Pages: 18 - 24
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Shanmugaarasu Venkatachalam, Kathiresan Kandasamy, Ilanchelian Krishnamoorthy, Rajendran Narayanasamy
      The potential use of mangrove swamp for fish farming industry is not clearly known. Therefore, current study was conducted to assess the growth performance of the Asian Seabass, Lates calcarifer cultivated in integrated mangrove-aquaculture system (IMAS) and open aquaculture system without mangroves (OAS). Fish survival and biomass production were higher by 11% and 12.5% respectively in the IMAS than those in the OAS. The fish growth performance was higher in monsoon than that in other seasons. It was in association with water quality parameters such as, high levels of DO, chlorophylls-a,b, nitrate-N, DOC, TOC; low levels of light intensity, temperature (air, water), SPM, chlorophyll-c, nitrite-N, ammonia, total phosphate, reactive silicate, and POC; as well with moderate salinity. The water quality seemed to be favourable for growth and survival of the fish. Therefore, integrating the mangroves with fish farming of the Asian seabass is beneficial for better fish survival and biomass production.

      PubDate: 2017-12-11T10:11:12Z
      DOI: 10.1016/j.aqrep.2017.11.004
      Issue No: Vol. 9 (2017)
  • Successful large-scale hatchery culture of sandfish (Holothuria scabra)
           using micro-algae concentrates as a larval food source

    • Authors: Thane A. Militz; Esther Leini; Nguyen Dinh Quang Duy; Paul C. Southgate
      Pages: 25 - 30
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Thane A. Militz, Esther Leini, Nguyen Dinh Quang Duy, Paul C. Southgate
      This paper reports methodology for large-scale hatchery culture of sandfish, Holothuria scabra, in the absence of live, cultured micro-algae. We demonstrate how commercially-available micro-algae concentrates can be incorporated into hatchery protocols as the sole larval food source to completely replace live, cultured micro-algae. Micro-algae concentrates supported comparable hatchery production of sandfish to that of live, cultured micro-algae traditionally used in large-scale hatchery culture. The hatchery protocol presented allowed a single technician to achieve production of more than 18,800 juvenile sandfish at 40days post-fertilisation in a low-resource hatchery in Papua New Guinea. Growth of auricularia larvae fed micro-algae concentrates was represented by the equation length (μm)=307.8×ln(day)+209.2 (R2 =0.93) while survival over the entire 40day hatchery cycle was described by the equation survival =2× day −1.06 (R2 =0.74). These results show that micro-algae concentrates have great potential for simplifying hatchery culture of sea cucumbers by reducing infrastructural and technical resources required for live micro-algae culture. The hatchery methodology described in this study is likely to have applicability to low-resource hatcheries throughout the Indo-Pacific and could support regional expansion of sandfish hatchery production.

      PubDate: 2017-12-11T10:11:12Z
      DOI: 10.1016/j.aqrep.2017.11.005
      Issue No: Vol. 9 (2017)
  • Enhancement of dietary effect of Nannochloropsis sp. on juvenile Ruditapes
           philippinarum clams by alginate hydrolysates

    • Authors: Yasuhiro Yamasaki; Keita Ishii; Shigeru Taga; Masanobu Kishioka
      Pages: 31 - 36
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Yasuhiro Yamasaki, Keita Ishii, Shigeru Taga, Masanobu Kishioka
      The eustigmatophyte Nannochloropsis sp. can be produced on a large scale at low cost. However, its dietary usefulness for juvenile bivalves is less than that of other algae. Recently, we reported that growth of juveniles of the clam Ruditapes philippinarum was dramatically promoted by supplementing a diet of the diatom Chaetoceros neogracile with alginate hydrolysates (AHs) at the concentration of 4mgL−1. In this study, we examined the effect of AHs on Nannochloropsis sp. as a clam diet. Ten-day rearing experiments in 500-mL beakers showed that AHs have a beneficial effect on clam culture regardless of water temperature. Shell growth in clams given AHs at the concentration of 4mgL−1 along with Nannochloropsis sp. at the concentration of 20×104 cells mL−1 was higher than in any other test groups at 15 or 25°C. In 20-day rearing experiments in 30-L tanks, the average shell length in the groups given AHs at the concentration of 4mgL−1 along with Nannochloropsis sp. at the concentration of 30×104 cells mL−1 was significantly greater than that in the groups given C. neogracile and Nannochloropsis sp. alone (P≪ 0.05). Furthermore, the total weight of clams given AHs at the concentration of 4mgL−1 along with Nannochloropsis sp. at the concentration of 30×104 cells mL−1 was greater than that in the group given only Nannochloropsis sp. (P≪ 0.05). Hence, the enhanced dietary effect of a combination of Nannochloropsis sp. and AHs will help to shorten the rearing time of R. philippinarum and to provide a stable supply of algal diet.

      PubDate: 2017-12-21T14:37:04Z
      DOI: 10.1016/j.aqrep.2017.11.006
      Issue No: Vol. 9 (2017)
  • Host gut-derived probiotic bacteria promote hypertrophic muscle
           progression and upregulate growth-related gene expression of slow-growing
           Malaysian Mahseer Tor tambroides

    • Authors: Md Asaduzzaman; Ezzah Sofia; Abrar Shakil; Nayeema Ferdausy Haque; M. Nurul Absar Khan; Daisuke Ikeda; Shigeharu Kinoshita; Ambok Bolong Abol-Munafi
      Pages: 37 - 45
      Abstract: Publication date: February 2018
      Source:Aquaculture Reports, Volume 9
      Author(s): Md Asaduzzaman, Ezzah Sofia, Abrar Shakil, Nayeema Ferdausy Haque, M. Nurul Absar Khan, Daisuke Ikeda, Shigeharu Kinoshita, Ambok Bolong Abol-Munafi
      In modern aquaculture, dietary supplementation of probiotics is a novel approach for enhancing growth performance of slow-growing fish. However, the actual role of probiotics in regulating muscle growth at cellular and molecular levels in fish still needs to be clarified. In this study, we hypothesized that host gut derived probiotic bacteria would enhance cellular muscle growth, and upregulate growth-related gene expression in slow-growing Malaysian mahseer Tor tambroides. Therefore, three host-associated probiotics (Bacillus sp. AHG22, Alcaligenes sp. AFG22, and Shewanella sp. AFG21) were isolated from the gastro-intestinal tract of T. tambroides and screened based on their digestive enzyme activity. A fishmeal and casein based control diet (40% crude protein and 10% lipid) was formulated, and three different probiotic supplemented diets were prepared by immersing the control diet in each isolated host-derived bacteria, suspended in sterile phosphate buffered saline (PBS), to achieve a final concentration of approximately 1.0 × 108 CFU g−1 feed. Triplicate groups of T. tambroides juveniles (initial weight 1.39 ± 0.06 g) were stocked in twelve glass aquaria (100 L capacity) with stocking density of 20 individuals per aquarium. The feed was applied twice daily at 3.0% of the fish body weight per day for 90 days. Growth performance (weight gain and specific growth rate) of T. tambroides juveniles were significantly higher in Alcaligenes sp. AFG22 and Bacillus sp. AHG22 supplemented diet treatments. Muscle morphometric analysis revealed that dietary supplementation of host-associated probiotic bacteria did not influence the frequency distribution of hyperplastic (class 10) small diameter fibers (≤10 μm). However, hypertrophic (Class 50, Class 60 and Class 70) large diameter fibers (>50 μm) were significantly higher in Alcaligenes sp. AFG22 and Bacillus sp. AHG22 supplemented treatments, indicating that increased growth rate of T. tambroides in these treatments was mostly governed by increased muscle fibers hypertrophy, rather than by hyperplasia. Real-time PCR data demonstrated that the relative mRNA expression of GH and IGF1 was upregulated in juvenile T. tambroides fed the diets supplemented with Alcaligenes sp. AFG22 and Bacillus sp. AHG22. These results indicate that host-derived probiotics, especially Alcaligenes sp. AFG22 and Bacillus sp. AHG22, have a significant potential as autochthonous probiotics for the stimulation of growth performance of slow-growing T. tambroides in aquaculture.

      PubDate: 2017-12-21T14:37:04Z
      DOI: 10.1016/j.aqrep.2017.12.001
      Issue No: Vol. 9 (2017)
  • Growing halophytes floating at sea

    • Authors: Ricardo Radulovich; María José Rodríguez; Rebeca Mata
      Pages: 1 - 7
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Ricardo Radulovich, María José Rodríguez, Rebeca Mata
      Freshwater shortages are increasingly limiting both irrigated and rainfed agriculture. To expand possibilities for controlled plant production without using land nor freshwater, we cultivated potted halophytes floating at sea that were provided with rain- and seawater. Plantlets of two mangroves (Avicennia germinans and Rhizophora mangle) and plants of two herbaceous species, sea purslane (Sesuvium portulacastrum) and salt couch grass (Sporobolus virginicus) were grown in near-coastal tropical Pacific waters of Costa Rica for 733days. There were a total of 504 rainless days, including two dry periods of ca. 150 d long each, evidencing prolonged and exclusive reliance on seawater. Pots with a sandy soil mixture and the transplanted plants were placed on low-cost wooden floating rafts with their lower end perforated and immersed for capillary rise of water. Free seawater entry and exit through the bottom from bobbing with waves, which also occasionally added water from the top, effectively controlled soil salinity build-up even during the rainless seasons. Continuous leaching made necessary frequent fertilizer addition. No water deficit symptoms were observed and midday canopy temperature during rainless periods was not significantly different between species or from air temperature. With all-year-round growth, height increase of mangrove plantlets ranged from 208.1 to 401.5mmyr−1. Fresh biomass production of sea purslane and the grass was 10.9 and 3.0kgm−2 yr−1 respectively. High yield, edibility and protein content of 10.2% dry weight established sea purslane as a potential crop. While further research is needed, the method evidenced to be a viable plant production option of potentially far-reaching applications.

      PubDate: 2017-08-13T10:20:03Z
      DOI: 10.1016/j.aqrep.2017.07.002
      Issue No: Vol. 8 (2017)
  • Effect of ammonia-N on histology and expression of immunoglobulin M and
           component C3 in the spleen and head kidney of Pelteobagrus vachellii

    • Authors: Chuan-jie Qin; Ting Shao; Yong-ming Wang; quan Gong; Qin Yang; Ping Bu
      Pages: 16 - 20
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Chuan-jie Qin, Ting Shao, Yong-ming Wang, quan Gong, Qin Yang, Ping Bu
      Ammonia-N is toxic to many aquatic animals and serves as a key stress factor in aquatic environments. The effects of ammonia-N stress on the immune response of darkbarbel catfish Pelteobagrus vachellii were investigated in this study. Changes in overall histology, and in the expression of complement C3 and immunoglobulin M (IgM) in spleen and head kidney, and lysozyme and C3 in serum, were measured in 1 and 5mg/L ammonia-N. Hyperemia, melano-macrophage assembly and loose splenosis were evident in spleen tissue. Both lysozyme and component C3 were significantly reduced in serum (P< 0.05) under both stress treatments, although lysozyme was increased slightly at 24h in the 1mg/L treatment. C3 mRNA expression increased at 6h in spleen and 6–12h in head kidney then decreased rapidly (P <0.05), although C3 mRNA recovered to control levels in spleen at 96h after the 1mg/L treatment (P >0.05). IgM expression also increased significantly at 6–12h in spleen and 6–24h in head kidney after the 1mg/L treatment (P< 0.05). A similar overall pattern were observed with 5mg/L ammonia (P< 0.05); IgM mRNA expression was elevated at 6h in spleen and 6–12h in head kidney (P< 0.05) then decreased to levels below controls (P< 0.05). These results suggest exposure to 5mg/L ammonia-N could damage the histological structure of spleen, diminish lysozyme and component C3 serum content, and suppress C3 and IgM expression in spleen and head kidney.

      PubDate: 2017-09-10T12:08:56Z
      DOI: 10.1016/j.aqrep.2017.08.001
      Issue No: Vol. 8 (2017)
  • Anti-oxidative effects of some dietary supplements on Yellow perch (Perca
           flavescens) exposed to different physical stressors

    • Authors: Hiam Elabd; Han-Ping Wang; Adel Shaheen; Hong Yao; Amany Abbass
      Pages: 21 - 30
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Hiam Elabd, Han-Ping Wang, Adel Shaheen, Hong Yao, Amany Abbass
      In current study, Yellow perch (P. flavescens) was exposed to common forms of physical stressors and antioxidative effects of dietary incorporated Astragalus membranaceus (AM) and Glycyrrhiza glabra (liquorice) were assessed. To address this, for a four-week five groups of fish (31±1.0g, average weight) received 1, 2, and 3% (w/w) Glycyrrhiza glabra; and 1% G. glabra- A. membranaceus mixture daily. Control group fed an additive-free basal diet. Immunological, biochemical and histopathological profiles were evaluated; and fish were redistributed to be exposed to heat, cold, hypoxia and capture stressors. The current findings demonstrated that A. membranaceus and G. glabra dietary incorporation remarkably enhanced antioxidative and biochemical parameters. Also, the study showed markedly up-regulation of related genes expression; and revealed better liver histology in supplemented groups over the control. In conclusion, A. membranaceus and G. glabra dietary supplementation markedly enhanced antioxidantive responses throughout the experimental period, indicating the ability of both herbal plants to confer protection against different physical stressors.

      PubDate: 2017-09-16T12:35:50Z
      DOI: 10.1016/j.aqrep.2017.09.002
      Issue No: Vol. 8 (2017)
  • Effects of environmental factors on growth, survival, and metamorphosis of
           geoduck clam (Panopea japonica A. Adams, 1850) larvae

    • Authors: Zhongming Huo; Haowen Guan; M. Golam Rbbani; Youxiang Xiao; Xuekai Zhang; Chao Fan; Zhuang Li; Ying Li; Qidi Wu; Feng Yang; Xiwu Yan
      Pages: 31 - 38
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Zhongming Huo, Haowen Guan, M. Golam Rbbani, Youxiang Xiao, Xuekai Zhang, Chao Fan, Zhuang Li, Ying Li, Qidi Wu, Feng Yang, Xiwu Yan
      A series of experiments were conducted to evaluate the effects of temperature, salinity, diet, and stocking density on the growth, survival, and metamorphosis of geoduck clam Panopea japonica larvae. The larvae all died at a temperature of 22°C after day 12, suggesting that the larvae of P. japonica could not survive when the temperature was higher than 22°C. P. japonica could be incubated at 19°C for the fast growth, high survival and metamorphosis of larvae. The embryos all died when the salinity was below 25 ppt. The larvae showed poor survival when the salinity was below 25 ppt, with all larvae dying before day 12, suggesting that larvae are sensitive to low salinity. The optimum salinity for the growth, survival and metamorphosis of larvae was 32 ppt. The use of a mixture of Isochrysis galbana and Nitzschia closterium (1:1) as a food source for the P. japonica larvae improved their growth, survival, and metamorphosis. A density of 20 individuals/ml appeared to be optimal for normal D-larvae of Panopea japonica, and 3–9 larvae/ml was optimal for the growth and survival of the P. japonica larvae raised in the hatchery.

      PubDate: 2017-09-21T12:45:41Z
      DOI: 10.1016/j.aqrep.2017.09.001
      Issue No: Vol. 8 (2017)
  • Vibrio parahaemolyticus a causative bacterium for tail rot disease in
           ornamental fish, Amphiprion sebae

    • Authors: Thangapandi Marudhupandi; Thipramalai Thankappan Ajith Kumar; Sanjeevi Prakash; Jeyagoby Balamurugan; Nagarajan Balachandran Dhayanithi
      Pages: 39 - 44
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Thangapandi Marudhupandi, Thipramalai Thankappan Ajith Kumar, Sanjeevi Prakash, Jeyagoby Balamurugan, Nagarajan Balachandran Dhayanithi
      The present study was performed to identify the tail rot disease causing bacterium in marine ornamental fish, Amphiprion sebae. Bacteria were isolated from the infected immune organs and tail region of A. sebae. Five different bacterial isolates (S1-S5) with different shape, size and colour were chosen for the infection study. The isolated strains were individually challenged with A. sebae at a constant dose of 1×107 CFU/fish. The virulent strain was found to be S-3, which showed maximum reproducing ability in A. sebae by causing typical tail rot disease and mortality. Furthermore, S-3 strain was identified as Vibrio parahaemolyticus by 16S rRNA gene sequencing (KF738005), biochemical analysis and amplification of tox R gene. Subsequently, extracellular products (ECPs) of V. parahaemolyticus were prepared by cellophane overlay method. The LD50 value of V. parahaemolyticus and its ECPS were found to be 1×105 CFU and 5μg/fish. The histology results revealed that V. parahaemolyticus and its ECPS are the major cause of tail rot disease in A. sebae.

      PubDate: 2017-10-05T14:26:58Z
      DOI: 10.1016/j.aqrep.2017.09.004
      Issue No: Vol. 8 (2017)
  • Effect of dietary supplements in American bullfrogs reared in low and high
           stocking densities

    • Authors: Jorgina Juliana Gradisse Freitas; Priscila Viau; Claudio Alvarenga Oliviera; Patricia Coelho Teixeira; Leonardo Tachibana; Danielle de Carla Dias; Marcio Hipolito; Isabella Cristina C. Bordon; Sthefany Rosa Alfaia; Claudia Maris Ferreira
      Pages: 45 - 48
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Jorgina Juliana Gradisse Freitas, Priscila Viau, Claudio Alvarenga Oliviera, Patricia Coelho Teixeira, Leonardo Tachibana, Danielle de Carla Dias, Marcio Hipolito, Isabella Cristina C. Bordon, Sthefany Rosa Alfaia, Claudia Maris Ferreira
      The aim of this study was to evaluate the effect of the probiotic Bacillus subtillis and beta-glucan from the fungus Agaricus blazei on survival, growth and immunological capacity in bullfrogs (Lithobates catesbeianus) cultured in low and high stocking densities. Animals weighing 24.3±2.38g were randomly distributed into four treatments with four simultaneous replicates: D100: 100 frogs/m2 (control); D236: 236 frogs/m2; D236+Prob.: 236 frogs/m2 supplemented with probiotic; and D236+BG: 236 frogs/m2 supplemented with beta-glucan. The parameters evaluated were weight gain, survival, plasma corticosterone (CORT), phagocytic capacity (PC) and phagocytic index (PI), at 24h and 15 and 30days. There is significant interaction between treatments and time for CORT levels. At 30days, these values were very close for the D100 (control) and D236+BG groups. Meanwhile, no statistical differences were observed between treatments for PC and PI. These results indicate that beta-glucan reduced the effects of stress caused by high density in bullfrogs, but the probiotic did not reduce these effects. Both compounds are not efficient at increasing survival rates, weight gain and neither immune response of animals. Thus, the use of commercial food additives may not have the favorable impact desired by the farmer. Their use in aquaculture should be further studied in experiments involving a longer trial period and taking into account the cost of their use.

      PubDate: 2017-10-05T14:26:58Z
      DOI: 10.1016/j.aqrep.2017.09.003
      Issue No: Vol. 8 (2017)
  • Measurement of gastrointestinal passage rate in Atlantic salmon (Salmo
           salar) fed dry or soaked feed

    • Authors: Turid Synnøve Aas; Hanne Jorun Sixten; Marie Hillestad; Harald Sveier; Trine Ytrestøyl; Bjarne Hatlen; Torbjørn Åsgård
      Pages: 49 - 57
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Turid Synnøve Aas, Hanne Jorun Sixten, Marie Hillestad, Harald Sveier, Trine Ytrestøyl, Bjarne Hatlen, Torbjørn Åsgård
      A method for measurement of gastrointestinal passage rate was tried in Atlantic salmon, Salmo salar (body weight 1131g, temperature 13.5°C, salinity 32‰). Salmon were force fed one single ration (10g) extruded feed ‘as is’ (92% dry matter) or soaked 2h in sea water (70% dry matter), in triplicate with three individuals per replicate. Content from stomach, small intestine and distal intestine was collected at 2, 6, 12, 18, 24 and 48h after feeding. Two hours after feeding, significantly more feed was transferred from the stomach to the small intestine in salmon fed soaked feed than in those fed dry feed. After that, no significant differences in gastrointestinal passage rate were found. Numerically however, soaked feed seemed to pass through the gut faster than dry feed. The content in stomach declined gradually, and all stomachs were empty after 48h. Salmon in both treatment groups used 6–12h on average to empty the stomach 50%. The content in small intestine peaked at the sampling 12h post feeding for salmon fed both feed types. After 48h, the small intestines were empty in all three replicates of salmon fed soaked feed, and in one replicate fed dry feed. The largest amounts of dry matter in the distal intestine were found between 12 and 24h for both feed groups. Some dry matter was still present in the hindgut after 48h. Individual variation in the gastrointestinal passage rate was large and few significant differences were revealed. However, soaked feed gave a significantly higher gastric evacuation rate than dry feed shortly (2h) after feeding. As the fish regurgitated some pellets, the method may be most suitable when feeding small rations. Besides, due to variation a larger number of replicates would increase the power of the test.

      PubDate: 2017-11-18T07:59:04Z
      DOI: 10.1016/j.aqrep.2017.10.001
      Issue No: Vol. 8 (2017)
  • Effect of stocking density and source of animal protein on growth and
           survival of rainbow trout fingerlings in flow-through system at Nuwakot,

    • Authors: Prem Timalsina; Choudhary Nagendra Roy Yadav; Gopal Prasad Lamsal; Krishna Prasad Acharya; Narayan Prasad Pandit
      Pages: 58 - 64
      Abstract: Publication date: November 2017
      Source:Aquaculture Reports, Volume 8
      Author(s): Prem Timalsina, Choudhary Nagendra Roy Yadav, Gopal Prasad Lamsal, Krishna Prasad Acharya, Narayan Prasad Pandit
      An experiment was conducted in outdoor nursing raceways with flow-through system (2.7m2) at the Fisheries Research Station, Trishuli, Nuwakot Nepal for 249days to evaluate the effect of stocking density and sources of animal protein on growth and survival of rainbow trout fingerlings production. The experiment was conducted in 2×2 factorial completely randomized design having two stocking densities, (density-1: 10kg/m2 and density-2: 12.5kg/m2) and two diets (diet-1: shrimp meal based diet and diet-2: 5% bovine blood meal mixed diet). All treatments were replicated thrice. Water from the Trishuli river was used and 50cm water depth was maintained in all treatments. Initial feeding rate of 10% of the body weight was gradually reduced to 8, 7, 6, 5, 4 and 3% was maintained for the subsequent months. Feeding was done 5 times a day throughout the study period. Results showed that the mean total harvest weight in diet-1 (30.17±1.34kg) was significantly higher than in diet-2 (22.77±1.34kg); however, no significance difference was observed at stocking density levels. Mean survival of fish in diet-1 (60.30±2.08%) was significantly higher than diet-2 (47.78±2.08%). Similarly, survivability of fish in density-1 (63.45±2.08%) was significantly higher than in density-2 (44.63±2.08%). The mean dissolve oxygen at density-2 (8.89±0.02mg/L) was significantly lower to that of density-1 (8.94±0.02mg/L) and B:C ratio was high with shrimp meal based diet and high stocking density (T3). In the present study, the treatment with shrimp meal based diet and high stocking density (T3) was superior with high mean total harvest weight, gross fish yield, low FCR and high B: C ratio than other treatment combinations. The present study demonstrated that growth, production and survival performances of rainbow trout in the present experimental condition were not satisfactory by substituting a part of shrimp meal by blood meal.

      PubDate: 2017-11-18T07:59:04Z
      DOI: 10.1016/j.aqrep.2017.10.002
      Issue No: Vol. 8 (2017)
  • Metabolomic analysis of marine and mud crabs based on antibacterial

    • Authors: A.A. Laith; M. Ambak; A.B. Abol-Munafi; W.W.I. Nurhafizah; M. Najiah
      Pages: 7 - 15
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): A.A. Laith, M. Ambak, A.B. Abol-Munafi, W.W.I. Nurhafizah, M. Najiah
      Isolated compounds from marine invertebrates are being increasingly known to possess various pharmacological activities with which many useful drugs have been developed. Crabs contain bioactive compounds including antibacterial, antifungal and antiviral metabolites, isolated from various tissues and organs that have revolutionized treatment of serious diseases. The present study represents the first attempt to investigate and compare the natural antibacterial properties from whole extract of marine blue swimmer crab, Portunus pelagicus, and mud crab, Scylla tranquebarica, against fish pathogenic bacteria. Liquid chromatography/mass spectrometry utilizing a time-of-flight (TOF) mass analyser (LC/MS-QTOF) based metabolomics approach was used to characterize the variation in secondary metabolite production in P. pelagicus and S. tranquebarica crab habitats in Malaysia. Different metabolites are evaluated in both crab species using LC/MS-QTOF. Initially a total of 75 metabolites were identified and only 19 metabolites satisfied the P-Corr cut-off point of less than 0.01 and at least 2-fold change. These metabolites, which contain anti-inflammatory and antibacterial properties, were down regulated in S. tranquebarica samples and up regulated in P. pelagicus samples. In vitro bioassay of methanolic P. pelagicus extracts showed the best antimicrobial response against Gram positive bacteria, Streptococcus agalactiae, and Gram negative bacteria, Vibrio alginolyticus, Klebsiella pneumoniae, and Escherichia coli, with a statistically significant difference (P< 0.05) of P. pelagicus extracts as compared to S. tranquebarica. The results indicate that both types of crab extracts are bactericidal at higher concentrations and bacteriostatic at lower concentrations. This manuscript reports the role of marine and mud crabs with specific emphasis on their secondary metabolites, and discusses current and future developments in both the production of desired crab metabolites and their potential uses in pharmaceutical industries.

      PubDate: 2017-05-20T12:55:18Z
      DOI: 10.1016/j.aqrep.2017.05.002
      Issue No: Vol. 7 (2017)
  • Effect of fish vitellogenin on the growth of juvenile catfish, Clarias
           gariepinus (Burchell, 1822)

    • Authors: Subir Kumar Juin; Shrabanti Sarkar; Sudipta Maitra; Panchanan Nath
      Pages: 16 - 26
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Subir Kumar Juin, Shrabanti Sarkar, Sudipta Maitra, Panchanan Nath
      The effect of heterologous fish vitellogenin (Vg)- implantation on growth performance in the juveniles of the catfish, Clarias gariepinus, was evaluated under ambient photoperiod and temperature during February–April (experiment 1) and again at May–August (experiment 2). Compared to either unimplanted (control) or bovine serum albumin (BSA)-implanted group, juveniles (average body weight: 3.5–4g) implanted (im) with Vg pellets (each containing 100μg of Clarias batrachus Vg, three per fish, at one month interval), revealed significantly (P< 0.05) higher specific growth rate (SGR) at 90days during both time frames. Congruent with accelerated somatic growth due to Vg-implantation, a trend of increase in plasma level of growth hormone (GH), estradiol-17β (E2) and testosterone (T) was recorded during expt. 1. While plasma E2 and T did not vary significantly, Vg-implantation promoted significant (P< 0.05) increase in plasma GH level in both the sexes during the course of expt. 2. Moreover, Vg-implantation could trigger significant alteration in gonadal growth; while control testis contained primarily spermatogonia (SG) and spermatocytes (SC), appearance of spermatozoa (SZ) was noticed in either Vg-implanted (expt. 1) or both BSA- and Vg-treated juveniles (expt. 2). Conversely, a robust increase in S III yolky oocytes was observed in Vg-implanted ovary during May–Aug; but not during expt. 1 (Feb–Apr). However, oocytes at earlier stages (S I and/or S II) of follicular growth and development were predominant in control ovary. Though the underlying mechanism is not yet clearly understood, present data indicate that implantation of Vg pellets in juvenile C. gariepinus may influence somatic growth indices; e.g., body weight gain and GH synthesis along with precocious gonadal growth in a manner sensitive to gonadal steroidogenesis.

      PubDate: 2017-05-25T14:08:04Z
      DOI: 10.1016/j.aqrep.2017.05.001
      Issue No: Vol. 7 (2017)
  • Long-term maintenance of the sea urchin Paracentrotus lividus in culture

    • Authors: Paola Cirino; Martina Ciaravolo; Angela Paglialonga; Alfonso Toscano
      Pages: 27 - 33
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Paola Cirino, Martina Ciaravolo, Angela Paglialonga, Alfonso Toscano
      The common sea urchin Paracentrotus lividus (Lamarck, 1816) is an important commercial species in the Mediterranean Sea for the consumption of its gonads (roe). This species has also long been used as an animal model in developmental biology and as an indicator in the assessment of environmental quality. In recent decades, the exploitation of this marine resource has become increasingly intensive, causing the depletion of wild stocks. The ripple effect observed in the laboratory use of this species has been the growing difficulty in finding valiant mature animals in the wild. We focused on the long-term maintenance of wild P. lividus and on the essential question of diet to maintain the animals and improve gonad development. The use of practical ration blocks which are nutrient-rich and show stability, easy storage and handling, resulted reduction in labor requirement and time for feeding streamlining the feeding practice. A significantly higher gonad production and a prolonged period of reproduction were obtained compared to wild caught individuals over the same period of time.

      PubDate: 2017-06-05T17:07:27Z
      DOI: 10.1016/j.aqrep.2017.04.003
      Issue No: Vol. 7 (2017)
  • Nutritional value of toasted pigeon pea, Cajanus cajan seed and its
           utilization in the diet of Clarias gariepinus (Burchell, 1822) fingerlings

    • Authors: Solomon Gabriel Solomon; Victor Tosin Okomoda; Samson Omirenya Oda
      Pages: 34 - 39
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Solomon Gabriel Solomon, Victor Tosin Okomoda, Samson Omirenya Oda
      The nutritional value of toasted pigeon pea Cajanus cajan seed in the diet of African catfish Clarias gariepinus (Burchell, 1822) was investigated in this study by progressively increasing its inclusion level (100, 200, 300 and 400gkg−1) in isonitrogenous (35% crude protein) and isoenergetic (17.7kJg−1) diets. Toasting of the C. cajan seed significantly reduced the anti-nutrients and increased most essential amino acid, protein, and fibre in the seeds. The growth of C. gariepinus (1.36±0.05g) fingerlings fed in triplicate 1×1×1m3 hapa pond system (n =40 in triplicates) was significantly improved by the different inclusion levels of toasted C. cajan in the diets after 56days. Comparing the performance of the fish fed 400gkg−1 of toasted C. cajan with that fed raw seed (400gkg−1) reveal the efficacy of this processing method in improving the utilization of the feedstuff. Mortality was significantly higher (32%) in fish fed the diet containing raw inclusion compared to that fed the control diet or inclusion of toasted C. cajan (<5%). Cost analysis revealed that it was economically cheaper to raise the African catfish using toasted C. cajan at 400gkg−1. It was therefore concluded that toasting improves the nutritional quality of C. cajan, resulting in better performance at higher inclusion levels, hence, can possibly reduce the cost of catfish production.

      PubDate: 2017-06-05T17:07:27Z
      DOI: 10.1016/j.aqrep.2017.05.005
      Issue No: Vol. 7 (2017)
  • Use practices of antimicrobials and other compounds by shrimp and fish
           farmers in Northern Vietnam

    • Authors: Tran Thi Kim Chi; Jesper H. Clausen; Phan Thi Van; Britt Tersbøl; Anders Dalsgaard
      Pages: 40 - 47
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Tran Thi Kim Chi, Jesper H. Clausen, Phan Thi Van, Britt Tersbøl, Anders Dalsgaard
      Aquaculture production is increasing in Vietnam, but is hampered by frequent disease outbreaks and widespread use of various compounds used to treat the fish and shrimp. The objective of this study was to analyse factors influencing farmer use practices of antimicrobials and other compounds by a questionnaire and observational survey conducted with 60 whiteleg shrimp (Litopenaeus vannamei) and 25 fish farmers in three coastal provinces in Northern Vietnam. Personnel in 22 shops distributing feed and chemicals for aquaculture were interviewed about their advice on sale to the farmers. Results showed that 20 different antimicrobial products were used for disease prevention and treatment in shrimp and marine fish culture. Banned products used included chloramphenicol, enrofloxacin and malachite green. Cage fish farmers said they purchased antimicrobial tablets readily available at a local pharmacy and sold for human use. Chinese traders were the main drug suppliers to the shrimp farmers in Quang Ninh and others provinces. Their products were sold with labels and product information written in Chinese only. Farmers appeared to have little awareness and concern about the disease aetiology when applying specific antimicrobials. Up to 50% of the shrimp farmers used up to 20 different disinfectants, e.g. chlorine-based compounds, to disinfect water in storage ponds, often without knowledge of the type of disinfectants and their mode of action. A variety of probiotics, vitamins, minerals and herbal extracts were routinely used by mainly shrimp farmers to enhance shrimp immunity. There is an urgent need to provide aquaculture farmers access to diagnostic and independent disease control advisory services and quality medicated feed, since the current indiscriminate use of antimicrobials and other compounds are inefficient, costly, and hazardous to the aquatic animal and farmer’s health, the environment and food safety.

      PubDate: 2017-06-09T17:28:08Z
      DOI: 10.1016/j.aqrep.2017.05.003
      Issue No: Vol. 7 (2017)
  • Egg quality traits and predictors of embryo and fry viability in red
           snapper Lutjanus campechanus

    • Authors: Agnès Bardon-Albaret; Eric Saillant
      Pages: 48 - 56
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Agnès Bardon-Albaret, Eric Saillant
      The quality of red snapper eggs is highly variable and unpredictable in aquaculture, leading to high mortality during early larval rearing. In this work, the viability of red snapper eggs was monitored from fertilization until unfed larvae expired because of exhaustion of vitelline reserves to determine egg quality traits in this species. The spawns were obtained via strip spawning wild-caught females following hormonal induction with chorionic gonadotropin. Females were induced immediately after capture (wild group, n=17) or held captive for the entire maturation period prior to induction (captive group, n=7). Candidate predictors of egg quality measured on the female parent at the time of induction or on the spawn at ovulation were evaluated using correlation and multiple regression analysis. The fertilization rate, the hatching rate, and the duration of survival of unfed larvae post hatch were weakly correlated to each other (-0.23< r< −0.08), revealing occurrence of distinct and independent components of egg quality. Spawns from captive females were characterized by a longer latency interval between hormonal induction and ovulation, lower fecundity, and lower hatching rates, as compared to those from wild females. Among the wild brood fish, a positive correlation was observed between the age of the female and the hatching rate. The best model optimized during stepwise multiple regression analysis of hatching rate data only explained 34% of the variance for this trait and no model could be optimized for the prediction of fertilization rate or the duration of survival post hatch. These results highlight the need to develop alternative egg quality measures to predict the viability of fry with confidence.

      PubDate: 2017-06-14T18:45:10Z
      DOI: 10.1016/j.aqrep.2017.05.004
      Issue No: Vol. 7 (2017)
  • Light and scanning electron microscopic studies of Myxobolus indica n. sp.
           and a report of three Myxozoan (Myxosporea: Bivalvulida) parasites of
           cultured ornamental goldfish, Carassius auratus L. for the first time in

    • Authors: Mandira Saha; P.K. Bandyopadhyay
      Pages: 66 - 76
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Mandira Saha, P.K. Bandyopadhyay
      The ornamental fish industry is an economically viable sector in India which suffers from different ectoparasitic infestations, including the myxozoan parasites. An icthyoparasitological survey of myxozoan infections in ornamental fish farms in India revealed the presence of four myxozoan parasites belonging to the family Myxobolidae, in the genera Myxobolus and Thelohanellus. The myxozoan spores were small to large, spherical to ellipsoidal in size. The plasmodia measured 0.5–3.0mm in diameter with disporic pansporoblasts and mature spores. During the survey the authors identified for the first time in India, three previously described species, namely, M. mehlhorni, T. nikolskii and T. batae; and one new species M. indica n. sp., all infecting the ornamental goldfish, Carassius auratus. The present study thus reports a new host, and a new locality for T. batae and M. mehlhorni. The description of T. nikolskii is the first record found in India. The spore of M. indica n. sp. measures 5.8±0.2×4.1±0.5μm in size, having two equal shaped pyriform polar capsules measuring 4.1±0.4×2.7±0.6μm. The results from a combination of light and scanning electron microscopic observations along with a comparison with closely related species were incorporated here. Molecular data is needed to complete the description of the new species.
      Graphical abstract image

      PubDate: 2017-06-19T19:38:08Z
      DOI: 10.1016/j.aqrep.2017.05.006
      Issue No: Vol. 7 (2017)
  • Valuation of vegetable crops produced in the UVI Commercial Aquaponic

    • Authors: Donald S. Bailey; Rhuanito S. Ferrarezi
      Pages: 77 - 82
      Abstract: Publication date: August 2017
      Source:Aquaculture Reports, Volume 7
      Author(s): Donald S. Bailey, Rhuanito S. Ferrarezi
      The UVI Commercial Aquaponic System is designed to produce fish and vegetables in a recirculating aquaculture system. The integration of these systems intensifies production in a small land area, conserves water, reduces waste discharged into the environment, and recovers nutrients from fish production into valuable vegetable crops. A standard protocol has been developed for the production of tilapia yielding 5 MT per annum. The production of many vegetable crops has also been studied but, because of specific growth patterns and differences of marketable product, no single protocol can be promoted. Each crop yields different value per unit area and this must be considered when selecting varieties to produce to provide the highest returns to the farmer. Variables influencing the value of a crop are density (plants/m2), yield (unit or kg), production period (weeks) and unit value ($). Combining these variables to one unit, $/m2/week, provides a common point for comparison among crops. Farmers can focus production efforts on the most valuable crops or continue to produce a variety of crops meeting market demand with the knowledge that each does not contribute equally to profitability.

      PubDate: 2017-06-26T20:57:01Z
      DOI: 10.1016/j.aqrep.2017.06.002
      Issue No: Vol. 7 (2017)
  • Effects of dietary lipid levels on growth, body composition and
           antioxidants of clamworm (Perinereis aibuhitensis)

    • Authors: Fu Lv; Fei Liu; Yebing Yu; Aimin Wang; Wenping Yang; Qing Nie; Tian Wang
      Pages: 1 - 7
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): Fu Lv, Fei Liu, Yebing Yu, Aimin Wang, Wenping Yang, Qing Nie, Tian Wang
      To determine the effects of dietary lipid levels on growth performance, body composition and antioxidant parameters of clamworm (Perinereis aibuhitensis), 1050 clamworms were fed diets with seven lipid levels (2.37%, 4.35%, 6.29%, 8.41%, 10.31%, 12.29% and 14.33%, named L2.37, L4.35, L6.29, L8.41, L10.31, L12.29 and L14.33, respectively) for 10 weeks. Each diet was randomly assigned to triplicate groups of 50 clamworms. The results showed that the growth performance and protein efficiency ratio were significantly affected by the lipid levels. Clamworms fed L8.41 diet exhibited higher growth performance than others and the maximum specific growth rate can be possibly obtained when the diets were supplemented with 7.54% lipid level. The dietary lipid levels had significant influences on the whole body crude protein, crude lipid, moisture contents and ash profile of P. aibuhitensis. The eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were also enhanced with increasing dietary lipid levels in whole body analyses. The contents of malonaldehyde (MDA) and lipid peroxidation (LPO) in clamworms increased significantly with increasing dietary lipid levels. Meanwhile, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total autioxidative capacity (T-AOC) tended to strengthen with dietary lipid levels increasing from 2.37% to 10.31% (except the GPX with 12.29% dietary lipid levels), and weaken with dietary lipid levels increasing from 10.31% to 14.33%. These results demonstrated that a proper dietary lipid level of 7.54%–10.31% could maintain solid growth performance and antioxidant capacity of juvenile P. aibuhitensis.

      PubDate: 2017-02-19T17:53:06Z
      DOI: 10.1016/j.aqrep.2017.02.001
      Issue No: Vol. 6 (2017)
  • Effects of canarium fruit (Canarium odontophyllum) oil as a dietary lipid
           source for juvenile mahseer (Tor tambroides) performance

    • Authors: M.L. Bami; M.S. Kamarudin; C.R. Saad; A. Arshad; M. Ebrahimi
      Pages: 8 - 20
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): M.L. Bami, M.S. Kamarudin, C.R. Saad, A. Arshad, M. Ebrahimi
      Canarium is among the riverine fruits that are commonly found in the natural diet of Malaysian mahseer, Tor tambroides. The fruit contains a high percentage of lipids. This study was conducted to investigate the effects of canarium crude oil on the growth performance, body composition and fatty acid profile of juvenile T. tambroides. Five isonitrogenous (40% crude protein) diets containing varying canarium oil levels (0, 1.25, 2.5, 3.75 and 5%) were prepared. Crude palm oil (CPO) was used as the control. The juveniles (2.08±0.10g) were given the test diets for 12 weeks in triplicate groups. Diets containing canarium oil were found to be less accepted by the fish which led to a significantly lower (P< 0.05) daily feed intake than diet including 0% canarium oil. Juveniles fed on 1.25–5% canarium oil also showed significantly lower (P<0.05) weight gain, specific growth rate, and protein efficiency ratio than those fed 0% canarium oil. However, the canarium oil level did not have any significant effect (P>0.05) on survival and lean portion of juvenile T. tambroides. The fish fed canarium oil-free diet also had better tissue fatty acid profile (especially n-3 and n-6 PUFA) as well as better protein, lipid and energy retention than those fed diets including canarium oil. From the results, canarium oil extracted from the whole fruit was not recommended as a dietary lipid source for T. tambroides. Moreover, juveniles fed on 0% canarium oil (5% CPO) utilized dietary saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) more efficiently for their energy requirement than fish given canarium oil.

      PubDate: 2017-03-05T00:33:44Z
      DOI: 10.1016/j.aqrep.2017.02.002
      Issue No: Vol. 6 (2017)
  • Effects of dietary tannin on growth, feed utilization and digestibility,
           and carcass composition in juvenile European seabass (Dicentrarchus labrax

    • Authors: Marie-Hélène Omnes; Julien Le Goasduff; Hervé Le Delliou; Nicolas Le Bayon; Patrick Quazuguel; Jean H. Robin
      Pages: 21 - 27
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): Marie-Hélène Omnes, Julien Le Goasduff, Hervé Le Delliou, Nicolas Le Bayon, Patrick Quazuguel, Jean H. Robin
      Plant-based products in fish diets are valuable protein alternatives to fishmeal for the aquafeed industry. Many plant feed ingredients contain polyphenolic compounds, including tannins, which can have beneficial or adverse effects. The tolerable threshold of ingested tannins is unknown for marine carnivorous fishes. We studied the effects of tannic acid (TA) supplementation to the diet of juvenile European seabass (Dicentrarchus labrax) by measuring growth, feed utilization and digestibility, and carcass composition. We randomly allocated groups of fish (initial mean body weight of 10.2±0.7g; n =40 fish per tank) to 12 replicate cylindrical-conical tanks (three per treatment). The fish were assigned to one of four dietary treatments for five weeks: control diet (C) with tannin-free protein sources (mostly fishmeal as the base diet, containing 55.7% dry matter (DM) crude protein, gross energy 22.3kJg−1 DM) and three experimental diets supplemented with 10, 20, or 30gTAkg−1 (called TA1, TA2, and TA3, respectively). Tannin ingestion resulted in significantly decreased cumulative feed intake, growth, feed and protein efficiencies, apparent digestibility coefficients, hepatosomatic index, and carcass lipids. The protein digestibility in fish fed the 10gkg−1 tannin-containing diet was significantly lower than that in fish fed the control diet. This threshold should be taken into account when using novel terrestrial and aquatic plant ingredients for temperate marine fishes.

      PubDate: 2017-03-10T04:20:08Z
      DOI: 10.1016/j.aqrep.2017.01.004
      Issue No: Vol. 6 (2017)
  • Productive performance and digestibility in the initial growth phase of
           tambaqui (Colossoma macropomum) fed diets with different carbohydrate and
           lipid levels

    • Authors: L.C.G. Sandre; H. Buzollo; T.M.T. Nascimento; L.M. Neira; R.K. Jomori; D.J. Carneiro
      Pages: 28 - 34
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): L.C.G. Sandre, H. Buzollo, T.M.T. Nascimento, L.M. Neira, R.K. Jomori, D.J. Carneiro
      The use of dietary protein can be optimized by increasing diet energy, which can be achieved by adding non-protein nutrients such as carbohydrates and lipids. If incorporated in suitable amounts, these items can promote the protein sparing effect, reducing nitrogen excretion and improving the quality of fish farming effluents. The study assessed productive performance, body composition, nutrient and energy retention efficiency and digestibility of the omnivorous fish tambaqui (Colossoma macropomum) fed diets with three carbohydrate (410, 460 and 510gkg−1) and two lipid levels (40 and 80gkg−1) in the initial growth phase (juvenile weighing between 10 and 250g). The experiment was completely randomized, with six treatments and four replicas arranged in a 3×2 factorial design. The 1080 tambaqui tested (10.88±0.13g body weight) were randomly distributed into 24 tanks (500L; 45 fish/tank) and fed the test diets for 120days. The highest carbohydrate inclusion (510gkg−1) reduced food intake and fish growth. A protein sparing effect was observed in the growth of tambaqui fed 460gkg−1 carbohydrates since they showed higher weight gain, protein efficiency ratio, protein productive value and crude protein participation in weight gain. The increase in lipid levels from 40gkg−1 to 80gkg−1 increased body fat deposition and decreased the digestibility coefficients of diet nutrients and diet energy. The results demonstrate that the ideal balanced diet to grow juvenile tambaqui is 460gkg−1 carbohydrates and 40gkg−1 lipids.

      PubDate: 2017-03-10T04:20:08Z
      DOI: 10.1016/j.aqrep.2017.02.003
      Issue No: Vol. 6 (2017)
  • Copepod swimming behavior, respiration, and expression of stress-related
           genes in response to high stocking densities

    • Authors: Birgitte Nilsson; Hans H. Jakobsen; Peter Stief; Guillaume Drillet; Benni W. Hansen
      Pages: 35 - 42
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): Birgitte Nilsson, Hans H. Jakobsen, Peter Stief, Guillaume Drillet, Benni W. Hansen
      Using copepod nauplii as live feed in aquaculture hatcheries could solve high mortality rates of first-feeding fish larvae due to malnutrition. However, implementing the use of copepod nauplii on an intensive production scale requires a stable production at preferably high densities, which is problematic for calanoid copepod species like Acartia tonsa. In the present study, we evaluated the response of copepods experiencing stress under high-density conditions by assessing the acute stress level of A. tonsa. Control density was at 100ind.L−1 while the treatments were increased stepwise up to 10,000ind.L−1. Three biological/physiological end-points were studied: swimming behavior, respiration rate and expression level of stress-related genes. None of the elevated densities caused any significant change in swimming behavior, respiration rate or gene expression level. This study suggests that adults of A. tonsa do not exhibit any measurable acute stress response when exposed to high culture densities for 12h.

      PubDate: 2017-03-10T04:20:08Z
      DOI: 10.1016/j.aqrep.2017.03.001
      Issue No: Vol. 6 (2017)
  • Comparison growth of Kappaphycus alvarezii (Rhodophyta, Solieriaceae)
           cultivation in floating cage and longline in Indonesia

    • Authors: Ma’ruf Kasim; Ahmad Mustafa
      Pages: 49 - 55
      Abstract: Publication date: May 2017
      Source:Aquaculture Reports, Volume 6
      Author(s): Ma’ruf Kasim, Ahmad Mustafa
      Currently, cultivation using cages for Kappaphycus alvarezii was limited reported before. This study aims to reveal growth of K. alvarezii cultivated in floating cages and longline. The study was conducted in one of cultivation areas in Southeast Sulawesi, Indonesia. Total gross weight, propagule growth rate and specific growth rate were performing in this study. During our field experiment, total gross weight of K. alvarezii after 40 days, from 5kg was growth to 22.5±1.40kg and 38.8±1.6kg on longline and floating cages, respectively. Propagule growth rate after 40 days, from 50g was 107.8±7.0g and 152.5±7.9g during April, and 132.0±8.0g and 218.8±8.6g during August, on longline and floating cage, respectively. Specific growth rate of K. alvarezii was high during August, 2.43%day−1 and 3.69%day−1 cultivated in longline and floating cage, respectively. Propagule morphology was white and damage in 40% cultivated by longline and no damage by using floating cage particular in August and September while high dense of herbivorous fish surrounding the experimental sites.

      PubDate: 2017-04-22T11:22:56Z
      DOI: 10.1016/j.aqrep.2017.03.004
      Issue No: Vol. 6 (2017)
  • Rainbow trout adaptation to a warmer Patagonia and its potential to
           increase temperature tolerance in cultured stocks

    • Authors: Sonia Alejandra Crichigno; Leandro Aníbal Becker; Mabel Orellana; Rodrigo Larraza; Guillermo Mirenna; Miguel Angel Battini; Víctor Enrique Cussac
      Abstract: Publication date: Available online 14 November 2017
      Source:Aquaculture Reports
      Author(s): Sonia Alejandra Crichigno, Leandro Aníbal Becker, Mabel Orellana, Rodrigo Larraza, Guillermo Mirenna, Miguel Angel Battini, Víctor Enrique Cussac
      The viability of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) culture is being challenged progressively by global warming. Previous trials with Australian and Japanese rainbow trout lines suggested that improvements in thermal performance may be possible. Here, we hypothesized that strain-related differences in physiological response to temperature exist between a north Patagonian hatchery stock (CENSALBA), a Neotropical one (Criadero Boca de Río), and a thermal stream (Valcheta) population of wild introduced rainbow trout. This was tested by comparing, at 20°C, the thermal preference, specific metabolic rate, thermal tolerance, growth, and condition on juveniles of the three strains, and on a Valcheta stream male x CENSALBA female F1 cross. Preferred temperature (PT) and loss of equilibrium temperature (LET, a measure of thermal tolerance) of Valcheta stream and F1 were significantly higher than those of CENSALBA, and the average PTs of Valcheta stream and F1 were higher than the 95% confidence interval of available reference data for rainbow trout. These results suggest that the F1, reared under standard hatchery conditions and selected by growth and thermal preference, presents higher thermal preference and higher thermal tolerance than the current CENSALBA hatchery stock. Introduction of this naturally adapted strain to hatchery stocks would likely result in the improvement of their temperature resistance to warmer waters. Current studies on adults of this F1 generation are underway.

      PubDate: 2017-11-18T07:59:04Z
      DOI: 10.1016/j.aqrep.2017.11.001
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