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Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 21)
Achievements in the Life Sciences     Open Access   (Followers: 4)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 23)
Acta Biologica Colombiana     Open Access   (Followers: 7)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 4)
Acta Biologica Sibirica     Open Access  
Acta Biomaterialia     Hybrid Journal   (Followers: 27)
Acta Biotheoretica     Hybrid Journal   (Followers: 5)
Acta Chiropterologica     Full-text available via subscription   (Followers: 6)
acta ethologica     Hybrid Journal   (Followers: 4)
Acta Limnologica Brasiliensia     Open Access   (Followers: 3)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales : The Journal of Silesian Museum in Opava     Open Access  
Acta Neurobiologiae Experimentalis     Open Access  
Acta Parasitologica     Hybrid Journal   (Followers: 9)
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Advanced Health Care Technologies     Open Access   (Followers: 4)
Advanced Studies in Biology     Open Access  
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
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African Journal of Range & Forage Science     Hybrid Journal   (Followers: 6)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 1)
Ageing Research Reviews     Hybrid Journal   (Followers: 9)
Aging Cell     Open Access   (Followers: 11)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat     Open Access  
AJP Cell Physiology     Full-text available via subscription   (Followers: 14)
AJP Endocrinology and Metabolism     Full-text available via subscription   (Followers: 23)
AJP Lung Cellular and Molecular Physiology     Full-text available via subscription   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Biology Teacher     Full-text available via subscription   (Followers: 13)
American Fern Journal     Full-text available via subscription   (Followers: 1)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 10)
American Journal of Bioethics     Hybrid Journal   (Followers: 10)
American Journal of Human Biology     Hybrid Journal   (Followers: 13)
American Journal of Medical and Biological Research     Open Access   (Followers: 8)
American Journal of Plant Sciences     Open Access   (Followers: 19)
American Journal of Primatology     Hybrid Journal   (Followers: 16)
American Malacological Bulletin     Full-text available via subscription   (Followers: 3)
American Naturalist     Full-text available via subscription   (Followers: 73)
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Analytical Methods     Full-text available via subscription   (Followers: 10)
Anatomical Science International     Hybrid Journal   (Followers: 2)
Animal Cells and Systems     Hybrid Journal   (Followers: 4)
Annales de Limnologie - International Journal of Limnology     Hybrid Journal   (Followers: 1)
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3)
Annales UMCS, Biologia     Open Access   (Followers: 1)
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Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Human Biology     Hybrid Journal   (Followers: 4)
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 16)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 25)
Annual Review of Cancer Biology     Full-text available via subscription   (Followers: 1)
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 39)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 16)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 20)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 10)
Anthropological Review     Open Access   (Followers: 24)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antibiotics     Open Access   (Followers: 9)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 8)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 8)
Applied Bionics and Biomechanics     Open Access   (Followers: 8)
Applied Vegetation Science     Full-text available via subscription   (Followers: 9)
Aquaculture Environment Interactions     Open Access   (Followers: 2)
Aquaculture International     Hybrid Journal   (Followers: 22)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 6)
Aquatic Biology     Open Access   (Followers: 5)
Aquatic Ecology     Hybrid Journal   (Followers: 32)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 14)
Aquatic Science and Technology     Open Access   (Followers: 3)
Aquatic Toxicology     Hybrid Journal   (Followers: 20)
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 3)
Archives of Biomedical Sciences     Open Access   (Followers: 7)
Archives of Microbiology     Hybrid Journal   (Followers: 8)
Archives of Natural History     Hybrid Journal   (Followers: 8)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 5)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arid Ecosystems     Hybrid Journal   (Followers: 3)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 2)
Arthropods     Open Access   (Followers: 1)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 3)
Artificial Photosynthesis     Open Access   (Followers: 1)
Asian Bioethics Review     Full-text available via subscription   (Followers: 2)
Asian Journal of Biodiversity     Open Access   (Followers: 5)
Asian Journal of Biological Sciences     Open Access   (Followers: 3)
Asian Journal of Cell Biology     Open Access   (Followers: 6)
Asian Journal of Developmental Biology     Open Access   (Followers: 2)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 2)
Asian Journal of Nematology     Open Access   (Followers: 3)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 6)
Autophagy     Hybrid Journal   (Followers: 2)
Avian Biology Research     Full-text available via subscription   (Followers: 5)
Avian Conservation and Ecology     Open Access   (Followers: 13)
Bacteriology Journal     Open Access   (Followers: 2)
Bacteriophage     Full-text available via subscription   (Followers: 4)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access   (Followers: 2)
Berita Biologi     Open Access   (Followers: 1)
Between the Species     Open Access   (Followers: 1)
Bio Tribune Magazine     Hybrid Journal  
BIO Web of Conferences     Open Access  
BIO-Complexity     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bioanalytical Reviews     Hybrid Journal   (Followers: 2)
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 6)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 14)
Biochimie     Hybrid Journal   (Followers: 7)
BioControl     Hybrid Journal   (Followers: 5)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal   (Followers: 1)
BioDiscovery     Open Access   (Followers: 2)
Biodiversity : Research and Conservation     Open Access   (Followers: 28)
Biodiversity and Natural History     Open Access   (Followers: 6)
Biodiversity Data Journal     Open Access   (Followers: 3)
Biodiversity Informatics     Open Access   (Followers: 1)
Biodiversity Information Science and Standards     Open Access  
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro     Open Access  
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 2)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 2)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
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BioéthiqueOnline     Open Access  
Biofabrication     Hybrid Journal   (Followers: 3)
Biogeosciences (BG)     Open Access   (Followers: 10)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 1)
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Biologia     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 5)
Biological Control     Hybrid Journal   (Followers: 4)
Biological Invasions     Hybrid Journal   (Followers: 17)
Biological Journal of the Linnean Society     Hybrid Journal   (Followers: 16)
Biological Letters     Open Access   (Followers: 4)
Biological Procedures Online     Open Access  
Biological Psychiatry     Hybrid Journal   (Followers: 42)
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Biologie in Unserer Zeit (Biuz)     Hybrid Journal   (Followers: 41)
Biologija     Open Access  
Biology     Open Access   (Followers: 5)
Biology and Philosophy     Hybrid Journal   (Followers: 17)
Biology Bulletin     Hybrid Journal   (Followers: 1)
Biology Bulletin Reviews     Hybrid Journal  

        1 2 3 4 5 6 7 8 | Last

Journal Cover Archives of Oral Biology
  [SJR: 0.713]   [H-I: 64]   [2 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0003-9969
   Published by Elsevier Homepage  [3118 journals]
  • Local administration of Tiludronic Acid downregulates important mediators
           involved in periodontal tissue destruction in experimental periodontitis
           in rats
    • Authors: Flávia A.C. Furlaneto; Nara L.T. Nunes; Ricardo B. Souza; Kely O. Yamamoto; Ivan Lima Oliveira Filho; Nicolly P.R. Frota; Hellíada Chaves; Mario Lisboa; Mario Taba Jr.; Edilson Ervolino; Michel R. Messora
      Pages: 1 - 9
      Abstract: Publication date: Available online 9 January 2018
      Source:Archives of Oral Biology
      Author(s): Flávia A.C. Furlaneto, Nara L.T. Nunes, Ricardo B. Souza, Kely O. Yamamoto, Ivan L. Oliveira Filho, Nicolly P.R. Frota, Hellíada Chaves, Mario Lisboa, Mario Taba, Edilson Ervolino, Michel R. Messora
      Objective The purpose of this study was to evaluate whether local administration of TIL could influence the expression of the inflammatory mediators IL-1β, TNF-α, MMP-8 and COX-2 in rats with experimental periodontitis (EP). Methods Twenty-four adult male rats (Rattus norvegicus, albinus, Wistar) were assigned to groups C, EP, EP-TIL (C–Control group, EP–Periodontitis groups). On EP groups, a ligature was placed around maxillary 2nd molars on day 1. On group EP-TIL, 20 μl of TIL solution (1 mg/kg body weight) was injected into the subperiosteal palatal area adjacent to the maxillary 2nd molar every other day until euthanasia (day 11). Alveolar bone loss was morphometrically analyzed. mRNA expressions of IL-1β, TNF-α, MMP-8 and COX-2 were assessed by qPCR. IL-1β, TNF-α, MMP-8 and COX-2 were immunohistochemically analyzed. Data were analyzed statistically. Results Group EP-TIL presented reduced alveolar bone loss when compared with group EP (p < 0.05). Group EP-TIL presented decreased mRNA expressions of IL-1β, TNF-α, MMP-8 and COX-2 and reduced immunolabeling of IL-1β, TNF-α and MMP-8 when compared with group EP (p<0.05). No differences regarding the immunolabeling of COX-2 were found when group EP-TIL was compared with the other groups (p>0.05). Conclusion Within the limits of this study, it can be concluded that local administration of TIL downregulates important mediators involved in periodontal tissue destruction in ligature-induced periodontitis in rats.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2018.01.005
      Issue No: Vol. 88 (2018)
  • Diagnostic potential and future directions of biomarkers in gingival
           crevicular fluid and saliva of periodontal diseases: Review of the current
    • Authors: Noha Ayman Ghallab
      Pages: 115 - 124
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Noha Ayman Ghallab
      Objectives The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival crevicular fluid (GCF) and saliva. Methods A literature search was performed using the Cochrane library and PubMed databases from 2000 to January 2017. Results Currently, there are more than 90 different components in the GCF that have been investigated as diagnostic and prognostic markers of periodontal disease progression involving; inflammatory mediators, markers of oxidative stress, host-derived enzymes, tissue-breakdown products and mediators of bone homeostasis. Furthermore, various biomarkers in saliva have been proposed which reveal a promising outlook for saliva as a key diagnostic medium for periodontal disease. Recent systematic reviews with high value of evidence have shown that potential salivary biomarkers can provide important complimentary diagnostic information and can be used as tests for screening diagnosis, prognosis and predicting periodontal disease progression. Conclusion Future developments in proteomic analysis and personalized medicine will pave the way allowing novel diagnostic tools. Still, the application into the field of dentistry will depend on how practitioners will apply this into their daily clinical practice. Clinical relevance Still, the application into the field of dentistry will depend on how practitioners will apply this into their daily clinical practice.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.022
      Issue No: Vol. 87 (2018)
  • MyD88-mediated innate sensing by oral epithelial cells controls
           periodontal inflammation
    • Authors: Andrea E. Delitto; Fernanda Rocha; Ann M. Decker; Byron Amador; Heather L. Sorenson; Shannon M. Wallet
      Pages: 125 - 130
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Andrea E. Delitto, Fernanda Rocha, Ann M. Decker, Byron Amador, Heather L. Sorenson, Shannon M. Wallet
      Periodontal diseases are a class of non-resolving inflammatory diseases, initiated by a pathogenic subgingival biofilm, in a susceptible host, which if left untreated can result in soft and hard tissue destruction. Oral epithelial cells are the first line of defense against microbial infection within the oral cavity, whereby they can sense the environment through innate immune receptors including toll-like receptors (TLRs). Therefore, oral epithelial cells directly and indirectly contribute to mucosal homeostasis and inflammation, and disruption of this homeostasis or over-activation of innate immunity can result in initiation and/or exacerbation of localized inflammation as observed in periodontal diseases. Dynamics of TLR signaling outcomes are attributable to several factors including the cell type on which it engaged. Indeed, our previously published data indicates that oral epithelial cells respond in a unique manner when compared to canonical immune cells stimulated in a similar fashion. Thus, the objective of this study was to evaluate the role of oral epithelial cell innate sensing on periodontal disease, using a murine poly-microbial model in an epithelial cell specific knockout of the key TLR-signaling molecule MyD88 (B6K5Cre.MyD88plox). Following knockdown of MyD88 in the oral epithelium, mice were infected with Porphorymonas gingivalis and Aggregatibacter actinomycetemcomitans by oral lavage 4 times per week, every other week for 6 weeks. Loss of oral epithelial cell MyD88 expression resulted in exacerbated bone loss, soft tissue morphological changes, soft tissue infiltration, and soft tissue inflammation following polymicrobial oral infection. Most interestingly while less robust, loss of oral epithelial cell MyD88 also resulted in mild but statistically significant soft tissue inflammation and bone loss even in the absence of a polymicrobial infection. Together these data demonstrate that oral epithelial cell MyD88-dependent TLR signaling regulates the immunological balance within the oral cavity under conditions of health and disease.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.016
      Issue No: Vol. 87 (2018)
  • Overexpression of sprouty2 in human oral squamous cell carcinogenesis
    • Authors: Pei-Hsien Liao; Yen-Yun Wang; Wen-Chen Wang; Chung-Ho Chen; Yu-Hsun Kao; Jing-Wei Hsu; Ching-Yi Chen; Ping-Ho Chen; Shyng-Shiou Yuan; Yuk-Kwan Chen
      Pages: 131 - 142
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Pei-Hsien Liao, Yen-Yun Wang, Wen-Chen Wang, Chung-Ho Chen, Yu-Hsun Kao, Jing-Wei Hsu, Ching-Yi Chen, Ping-Ho Chen, Shyng-Shiou Yuan, Yuk-Kwan Chen
      Objective This study investigated SPRY2 expression in human oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs). Methods 75 OSCCs, 23 OPMDs with malignant transformation (MT), 17 OPMDs without MT, and eight normal oral mucosa (NOM) tissues were used for immunohistochemical staining; three OSCC tissues with normal tissue counterparts were used for western blotting. Three human oral cancer cell lines (OCCLs), an oral precancer cell line (DOK), and a NOM primary culture (NOMPC) were used for western blotting; OCCLs and NOMPC were employed for real-time quantitative reverse transcription-polymerase chain reaction. OCCLs were evaluated in terms of proliferation, migration, invasion and BRAF V600E point mutation assays. Results Significantly increased SPRY2 protein expression was observed in OSCCs as compared with NOM, and SPRY2 expression also differed between OSCC patients with and without lymph-node metastasis. SPRY2 protein and mRNA expressions were significantly enhanced as compared with NOMPC. Increased phospho-ERK expression was observed in OCCLs as compared with NOMPC. Significant decreases in the proliferation rate, degrees of migration and invasion were noted in OCCLs with SPRY2 siRNA transfection as compared with those without SPRY2 siRNA transfection. No BRAF V600E point mutation was observed for OCCLs as compared with NOMPC. A significantly increased SPRY2 protein level was noted in OPMDs with MT as compared to those without MT, and was also found in OPMDs with MT in comparison with NOM, as well as in DOK in comparison with NOMPC. Conclusions Our results indicated that SPRY2 overexpression is associated with human oral squamous-cell carcinogenesis.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.021
      Issue No: Vol. 87 (2018)
  • Superhydrophilic co-polymer coatings on denture surfaces reduce Candida
           albicans adhesion—An in vitro study
    • Authors: Masahiro Hirasawa; Chiaki Tsutsumi-Arai; Kensuke Takakusaki; Toyohisa Oya; Kenji Fueki; Noriyuki Wakabayashi
      Pages: 143 - 150
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Masahiro Hirasawa, Chiaki Tsutsumi-Arai, Kensuke Takakusaki, Toyohisa Oya, Kenji Fueki, Noriyuki Wakabayashi
      Objective In this study, we aimed to investigate denture-base-resin coatings prepared with a crosslinkable co-polymer containing sulfobetaine methacrylamide (SBMAm) and the relationship between their surface characteristics and the initial adhesion of Candida albicans (C. albicans). Methods Acrylic resin discs were coated with co-polymers containing various concentrations of SBMAm and N,N’-(4,7,10-trioxa-1,13-tridecadiamine) diacrylamide (JDA) as crosslinking agent. Uncoated discs were used as controls. An acquired pellicle was formed on each disc using artificial saliva, and the discs were immersed in a suspension of C. albicans (JCM2085) cells. After incubation, tetrazolium salt (XTT-reduction) and colony forming unit (CFU) assays were performed and the morphogenesis of C. albicans was examined using scanning electron microscopy (SEM). The surface roughness, film thickness, and the water contact angle of each disc surface were measured. Results All coating groups showed significantly lower amounts of adhered C. albicans in the XTT-reduction and CFU assays than the control, confirmed by the SEM images. Many wrinkle structures were observed on the surfaces coated with co-polymers containing more than 30% SBMAm. There were no significant differences in surface roughness among all groups. The co-polymer films on the coated discs were less than 5.0 μm in thickness, and these surfaces exhibited significantly lower mean water contact angles than the control. Conclusion Crosslinkable co-polymers containing SBMAm can enhance the hydrophilicity of the surface of denture-base resins and reduce the initial adhesion of C. albicans.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.024
      Issue No: Vol. 87 (2018)
  • Immunoexpression of proteins involved in cytoskeleton remodeling in benign
           odontogenic lesions
    • Authors: Paula Nascimento Antonio; Natália Galvão Garcia; Agnes Assao; José Roberto Pereira Lauris; Fernando Augusto Soares; Denise Tostes Oliveira
      Pages: 151 - 156
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Paula Nascimento Antonio, Natália Galvão Garcia, Agnes Assao, José Roberto Pereira Lauris, Fernando Augusto Soares, Denise Tostes Oliveira
      Objective The present study was designed to analyze the immunolocalization of proteins involved in cytoskeleton remodeling, such as moesin and Rho-A, in benign odontogenic lesions that present with expansive growth and invasive clinical behavior. Materials and methods Expressions of moesin and Rho-A in odontogenic epithelium were evaluated by immunohistochemical analysis in 45 odontogenic lesions using monoclonal antibodies. Results Our results demonstrated strong membranous and cytoplasmic expressions of moesin in the epithelial cells in 66.7% and 44.4% of the odontogenic lesions, respectively. Furthermore, Rho-A expression in odontogenic epithelium was strong in the membrane and cytoplasm of 51.1% and 62.2% of the odontogenic lesions, respectively. A statistically significant correlation was found between the membranous and cytoplasmic expressions of moesin (p = 0.000) and those of Rho-A (p = 0.048) in odontogenic epithelial cells, while no statistically significant correlation was found between moesin and Rho-A expressions (p > 0.05). Conclusions The present study confirmed the strong expressions of moesin and Rho-A by odontogenic epithelial cells, suggesting their involvement in the development of benign odontogenic lesions. However, this study has failed to detect the connection between the moesin and Rho-A interaction in expansive growth and local invasiveness of these lesions.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.017
      Issue No: Vol. 87 (2018)
  • Bleaching and enamel surface interactions resulting from the use of
           highly-concentrated bleaching gels
    • Authors: Guillermo Grazioli; Lisia Lorea Valente; Cristina Pereira Isolan; Helena Alves Pinheiro; Camila Gonçalves Duarte; Eliseu Aldrighi Münchow
      Pages: 157 - 162
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Guillermo Grazioli, Lisia Lorea Valente, Cristina Pereira Isolan, Helena Alves Pinheiro, Camila Gonçalves Duarte, Eliseu Aldrighi Münchow
      Tooth bleaching is considered a non-invasive treatment, although the use of highly-concentrated products may provoke increased surface roughness and enamel demineralization, as well as postoperative sensitivity. Thus, the aim of this study was to investigate whether hydrogen peroxide (H2O2) concentration would affect tooth bleaching effectiveness and the enamel surface properties. Enamel/dentin bovine specimens (6 × 4 mm) were immersed in coffee solution for 7 days and evaluated with a spectrophotometer (Easyshade; baseline), using the CIEL*a*b* color parameters. Hardness was measured using a hardness tester. The specimens were randomly assigned into four groups: one negative control, in which the specimens were not bleached, but they were irradiated with a laser-light source (Whitening Lase II, DMC Equipments); and three groups using distinct H2O2 concentration, namely LP15% (15% Lase Peroxide Lite), LP25% (25% Lase Peroxide Sensy), and LP35% (35% Lase Peroxide Sensy), all products from DMC. The bleached specimens were also irradiated with the laser-light source. After bleaching, all specimens were evaluated using scanning electron microscopy (SEM). pH kinetics and rate was monitored during bleaching. The data were analyzed using ANOVA and Tukey’s test (p < 0.05). All bleaching gels produced similar color change (p > 0.05). Concerning hardness, only the LP25% and LP35% significantly reduced hardness after bleaching; also, there was a progressive tendency for a greater percentage reduction in hardness with increased H2O2 concentration of the gel (R2 = 0.9973, p < 0.001). SEM showed that LP25% and LP35% produced an etching pattern on enamel with prism rods exposure. In conclusion, H2O2 concentration above the 15% level does not increase bleaching effectiveness, and may increase the possibility for alteration of enamel hardness, surface morphology, and acidity of the medium. When using H2O2-based bleaching agents, dental practitioners should choose for less concentrated gels, e.g., around the 15% level.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.026
      Issue No: Vol. 87 (2018)
  • Pulpal blood flow recorded from exposed dentine with a laser Doppler flow
           meter using red or infrared light
    • Authors: Kanittha Kijsamanmith; Noppakun Vongsavan; Bruce Matthews
      Pages: 163 - 167
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Kanittha Kijsamanmith, Noppakun Vongsavan, Bruce Matthews
      Objective To determine the percentage of the blood flow signal that is derived from dental pulp when recording from exposed dentine in a human premolar. Design Recordings were made from 7 healthy teeth in 5 subjects (aged 22–33 yr.) with a laser Doppler flow meter (Periflux 4001) using either a red (635 nm) or an infrared (780 nm) laser. After exposing dentine above the buccal pulpal horn (cavity diam. 1.6 mm, depth 3 mm) and isolating the crown with opaque rubber dam, blood flow was recorded alternately with infrared or red light from the exposed dentine under four conditions: before and after injecting local anaesthetic (3% Mepivacaine without vasoconstrictor) (LA) over the apex of the root of the tooth; after exposing the pulp by cutting a buccal, class V cavity in the tooth; and after sectioning the coronal pulp transversely through the exposure. Results There was no significant change in mean blood flow recorded with either light source when the tooth was anaesthetized or when the pulp was exposed. After the pulp had been sectioned, the blood flow recorded with infrared light fell by 67.8% and with red light, by 68.4%. The difference between these effects was not significant. Conclusions When recording blood flow from exposed coronal dentine with either infrared or red light in a tooth isolated with opaque rubber dam, about 68% to the signal was contributed by the pulp. The signal:noise ratio was better with infrared than red light, and when recording from dentine than enamel.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.009
      Issue No: Vol. 87 (2018)
  • Association of weight and height with timing of deciduous tooth emergence
    • Authors: Ashraf I. Shaweesh; Ola B. Al-Batayneh
      Pages: 168 - 171
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Ashraf I. Shaweesh, Ola B. Al-Batayneh
      Objectives The aim of this study was to associate weight and height with the timing of deciduous tooth emergence. Methods 1756 children, aged from 1 to 33 months (755 females and 1001 males) had been previously examined for the timing of deciduous tooth emergence and their weights and heights measured. Children were categorized into weight and height groups (underweight, normal, overweight, short stature, normal and tall stature). Probit regression analysis was used to calculate the ages at emergence of each deciduous tooth. Tooth emergence ages were compared pairwise across the weight and height groups. Results The deciduous dentition emerged between 7.9–31.5, 7.8–29.6 and 6.3–26.5 months in the underweight, normal and overweight children, respectively. In the height groups, the ranges were 9.0–31.3, 7.4–27.0 and 6.7–24.3 in the short, normal and tall children, respectively. The extremes of weight and height were related to the times at deciduous tooth emergence where a substantial increase in weight or height was associated with earlier emergence, and vice versa. However, only canines, lateral incisors and upper central incisor showed statistically significant association with weight and none of the teeth exhibited statistically significant association with height. Moreover, no emergence sequence change was associated with weight and height. Conclusions The present study provides the first weight and height– specific data on the timing of deciduous tooth emergence. Although both weight and height are generally associated with the timing of deciduous tooth emergence, weight shows a stronger association for canines, lateral incisors and upper central incisor. The findings will aid assessing normal emergence timing in children with variant weights and heights.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.030
      Issue No: Vol. 87 (2018)
  • miR-143 suppresses the osteogenic differentiation of dental pulp stem
           cells by inactivation of NF-κB signaling pathway via targeting TNF-α
    • Authors: Peng Zhang; Wenli Yang; Guofang Wang; Yajing Li
      Pages: 172 - 179
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Peng Zhang, Wenli Yang, Guofang Wang, Yajing Li
      Background Dental pulp stem cells (DPSCs) are multipotent and play an important role in repairing damaged and/or defective dentinogenesis/osteogenesis. Recent studies have documented the implication of miR-143 in osteogenic differentiation of DPSCs. Nevertheless, the detailed mechanisms of miR-143 involved in the osteogenic differentiation of DPSCs remain to be further elaborated. Methods Isolated DPSCs were incubated with osteogenic differentiation medium to induce osteogenic differentiation. qRT-PCR and western blot were performed to determine the expressions of miR-143 and tumor necrosis factor α (TNF-α). Luciferase reporter assay was used to confirm whether TNF-α was a target of miR-143. Osteogenic differentiation of DPSCs was evaluated by alkaline phosphatase (ALP) activity assay, ALP staining, and western blot analyses of osteogenic-markers including bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (RUNX2) and collagen type I (COLI). Results miR-143 was downregulated and TNF-α was upregulated during osteogenic differentiation of DPSCs. miR-143 posttranscriptionally regulated TNF-α expression in DPSCs by binding to its 3′UTR. miR-143 overexpression suppressed osteogenic differentiation of DPSCs, as demonstrated by the decrease of ALP activity, ALP positive cell ratio, as well as BMP2, ALP, RUNX2, and COLI expressions. Moreover, miR-143 reversed TNF-α-induced osteogenic differentiation of DPSCs. Finally, the osteogenic differentiation of DPSCs induced by miR-143 inhibitor was attenuated following inactivation of nuclear factor kappa B (NF-κB) signaling pathway. Conclusion miR-143 suppressed the osteogenic differentiation of DPSCs by blockade of NF-κB signaling pathway via targeting TNF-α.

      PubDate: 2018-01-03T22:16:36Z
      DOI: 10.1016/j.archoralbio.2017.12.031
      Issue No: Vol. 87 (2018)
  • Association between developmental defects of enamel and celiac disease: A
    • Authors: Débora Souto-Souza; Maria Eliza da Consolação Soares; Vanessa Silva Rezende; Paulo César de Lacerda Dantas; Endi Lanza Galvão; Saulo Gabriel Moreira Falci
      Pages: 180 - 190
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Débora Souto-Souza, Maria Eliza da Consolação Soares, Vanessa Silva Rezende, Paulo César de Lacerda Dantas, Endi Lanza Galvão, Saulo Gabriel Moreira Falci
      Objectives Studies have observed the presence of extra-intestinal manifestations of celiac disease (CD), including involvement of the oral cavity, such that developmental defects of enamel (DDE) occur. Thus, the aim of this review was to access the polled prevalence of DDE in individuals with CD, and to establish the strength of the association between these two variables. Methods To carry out the systematic review, four electronic databases and the Grey Literature were searched, complemented by a manual search of reference lists within the selected articles. Two pairs of independent reviewers selected the articles, and perform the data extractions and bias risk assessment Studies evaluating the presence of DDE in individuals with CD as well as in healthy individuals and which performed the DDE diagnosis by direct visualization of tooth enamel changes and the CD diagnosis were included. Meta-analyses were performed using the software R. Results Of 557 studies, 45 were selected for review, encompassing 2840 patients. The prevalence of DDE in people with CD was 50% (95% CI 0.44–0.57, I2 = 88%). In a general analysis, it was observed that patients with CD had a significantly higher prevalence of enamel defects compared to healthy people (RR: 2.31, 95% CI: 1.71–3.12, I2 = 98%). Only developmental defects of enamel diagnosed using Aine’s method were associated with the disease (RR: 3.30, 95% CI 2.39–4.56, I2 = 75%). In a sensitivity analysis involving the deciduous, mixed and permanent dentitions, only individuals with deciduous dentition were observed to have association with the disease (RR: 2.34, 95% CI 1.25–4.39, I2 = 39%). Conclusions Patients with enamel developmental defects should be screened for the possibility of their having celiac disease.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2017.12.025
      Issue No: Vol. 87 (2018)
  • Histological and chemical analyses of mesiodens development and
    • Authors: Awady Muhamad; Moskovitz Moti; Cohen Ornit; Zilberman Uri
      Pages: 191 - 195
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Awady Muhamad, Moskovitz Moti, Cohen Ornit, Zilberman Uri
      Objective This study aimed to compare the developmental timing and mineralization quality of mesiodentes, i.e., supernumerary teeth located mainly in the midline of the maxilla between the central incisors, with the developmental timing and mineralization quality of permanent and primary central incisors. Design Sixteen mesiodentes, nine permanent and seven primary central incisors were collected. The location of the neonatal line was determined using a light microscope at 10× or 20× enlargements. Chemical composition of the enamel at two locations was analyzed using energy dispersive X-ray spectrometer. Results Neonatal lines were observed in eight out of 16 mesiodentes, in all primary central incisors and in none of the permanent central incisors. Chemical analyses showed that mesiodentes mineralization was impaired, resulting in higher amount of organic ions and reduced inorganic ions. Discriminant analysis showed minimal overlap of mesiodentes with either primary or permanent centrals. Conclusions Mesiodentes development begins before birth in 50% of the cases but later than the primary centrals. Mineralization of mesiodens is impaired with less mineral content and higher organic content. The results showed that mesiodentes are a special group of teeth with defective morpho-differentiation and mineralization, with little similarity to primary or permanent central incisors.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2017.12.020
      Issue No: Vol. 87 (2018)
  • The alveolar bone protective effects of natural products: A systematic
    • Authors: Irlan Almeida Freires; Gustavo Machado Santaella; Janaina de Cássia Orlandi Sardi; Pedro Luiz Rosalen
      Pages: 196 - 203
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Irlan Almeida Freires, Gustavo Machado Santaella, Janaina de Cássia Orlandi Sardi, Pedro Luiz Rosalen
      Objectives This systematic review was carried out to identify which naturally-occurring agents and constituents isolated therefrom have effects in preventing bone loss in a ligature-induced periodontitis model. Materials and methods Eight databases were systematically searched for studies of experimental periodontitis. The data were extracted, analyzed, and the treatment outcomes were given scores based on the level of bone destruction as compared to their untreated induced-periodontitis control. Results 294 articles were found, of which 15 met the inclusion criteria. The selected studies tested a multi-herbal formulation; extracts (leaves, barks or fruit) of different plant species; and propolis. The most usual dosing protocol consisted of 3-times-a-day, 11-day treatment. The combined gel of Myracrodruon urundeuva (5%) and Lippia sidoides (0.5%) was the most active treatment, reducing 45–65% bone loss in the region of molars as compared to 73.4% of doxycycline (gold-standard). Ginkgo biloba extract (28–56 mg/kg) and propolis (100–200 mg/kg) prevented bone destruction by 50% and 40–44%, respectively. The other tested samples showed intermediate/weak activity in modulating bone resorption. Conclusions The gel of M. urundeuva and L. sidoides, and G. biloba and propolis extracts showed strong alveolar bone protective effectiveness in induced-periodontitis in rats. Further translational research should bridge the gap between the rat study outcomes and the clinical efficacy and long-term toxicity of these formulations in humans. The compilation of the vast literature database presented herein may drive further in vivo and clinical studies with the selected efficacious formulations to subsidize their pharmaceutical application.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2017.12.019
      Issue No: Vol. 87 (2018)
  • Influence on proliferation and adhesion of human gingival fibroblasts from
           different titanium surface decontamination treatments: An in vitro study
    • Authors: Jie Cao; Tong Wang; Yinfei Pu; Zhihui Tang; Huanxin Meng
      Pages: 204 - 210
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Cao Jie, Tong Wang, Pu Yinfei, Tang Zhihui, Meng Huanxin
      Objectives To investigate the effects of different decontamination treatments on microstructure of titanium (Ti) surface as well as proliferation and adhesion of human gingival fibroblasts (HGFs). Material and methods Ti discs with machined (M) and sand blasted, acid etched (SAE) surfaces were treated with five different decontamination treatments: (1) stainless steel curette (SSC), ultrasonic system with (2) straight carbon fiber tip (UCF) or (3) metal tip (UM), (4) rotating Ti brush (RTB), and (5) Er:YAG laser (30 mJ/pulse at 30 Hz). Surface roughness was analyzed under optical interferometry. HGFs were cultured on each disc. Proliferation and adhesive strength were analyzed. qRT-PCR and ELISA were performed to detect the RNA and protein expression of FAK, ITGB1, COL1A1, and FN1 respectively from different Ti surfaces. Results Surface roughness increased on M surface. Proliferation, adhesive strength and gene expression were higher on M surface than SAE surface. Decontamination treatments affected surface parameters significantly (P < 0.001), making M surface less smooth while SAE surface became less rough. SSC, UCF, UM and RTB decreased proliferation on M surfaces significantly (P < 0.05). UCF, RTB and laser increased proliferation on SAE surface significantly (P < 0.05). UM decreased adhesive strength on M surface significantly and laser increased adhesive strength on SAE surface significantly (P < 0.05). Gene expression increased with time and was altered by decontamination treatments significantly (P < 0.001). Conclusions Decontamination treatments influence surface roughness and cell behavior of HGFs. Laser might be an optimal decontamination treatment which has the least negative effect on M surface and the most positive effect on SAE surface.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2017.12.013
      Issue No: Vol. 87 (2018)
  • Epigenetic mechanisms in odontogenic tumors: A literature review
    • Authors: Jorge Sandoval-Basilio; Rogelio González-González; Ronell Bologna-Molina; Mario Isiordia-Espinoza; Gabriela Leija-Montoya; Sofia L. Alcaraz-Estrada; Idanya Serafín-Higuera; Javier González-Ramírez; Nicolás Serafín-Higuera
      Pages: 211 - 217
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Jorge Sandoval-Basilio, Rogelio González-González, Ronell Bologna-Molina, Mario Isiordia-Espinoza, Gabriela Leija-Montoya, Sofia L. Alcaraz-Estrada, Idanya Serafín-Higuera, Javier González-Ramírez, Nicolás Serafín-Higuera
      Objective Epigenetic mechanisms, such as DNA methylation, regulate important biological processes as gene expression and it was suggested that these phenomena play important roles in the carcinogenesis and tumor biology. The aim of this review is to provide the current state of knowledge about epigenetic alterations, focusing mainly on DNA methylation, reported in odontogenic tumors. Design Literatures were searched based in the combination of the following keywords: odontogenic tumors, epigenetics, DNA methylation, histone modifications, non-coding RNA, microRNA, DNA methyltransferases. Electronic databases (Medline/PubMed, Scopus and Web of Science) were screened. Results The analysis of epigenetic alterations in different tumors has rapidly increased; however, limited information is available about epigenetic mechanisms involved in the formation of odontogenic tumors. DNA methylation is the most studied epigenetic modification in these tumors and the participation of non-coding RNA’s in odontogenic tumors has been recently addressed. Differential expression of DNA methyltransferases, altered DNA methylation patterns and aberrant expression of non-coding RNA’s were reported in odontogenic tumors. Conclusions Current studies suggest epigenetics as an emerging mechanism, possibly implicated in etiopathogenesis of odontogenic tumors. Deeper understanding of the epigenetic abnormalities in these tumors could show potential applications as biomarkers or therapeutic possibilities in the future.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2017.12.029
      Issue No: Vol. 87 (2018)
  • Topical chlorhexidine, povidone-iodine and erythromycin in the repair of
           traumatic ulcers on the rat tongue: Clinical, histological and
           microbiological evaluation
    • Authors: Dieni da Silveira Teixeira; Maria Antonia Zancanaro de Figueiredo; Karen Cherubini; Maria Claudia Rosa Garcia; Sílvia Dias de Oliveira; Fernanda Gonçalves Salum
      Pages: 218 - 225
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Dieni da Silveira Teixeira, Maria Antonia Zancanaro de Figueiredo, Karen Cherubini, Maria Claudia Rosa Garcia, Sílvia Dias de Oliveira, Fernanda Gonçalves Salum
      Objective This study investigated the effect of topical application of 0.12% chlorhexidine, 10% povidone-iodine and 50% erythromycin on the optimization of healing process of traumatic ulcers made on ventral tongue of rats. Design Forty-Eight Wistar rats were randomly divided into four groups: control, chlorhexidine (Chx), povidone-iodine (PvI) and erythromycin (Er). An ulcer of 5 mm in diameter was made on the ventral tongue of the animals. After 24 h, a microbiological sample was taken and daily application of the substances started. Six animals each group were euthanized at 4 days and the others at 8 days postoperative, totaling three and seven days of treatment. Prior to euthanasia, a new microbiological collection was performed. Results The experimental groups showed less area of residual ulcer. A significant difference was seen between the PvI and Chx in relation to the control after three days of treatment (p < 0.05). Although the experimental groups displayed greater newly formed epithelial area, there was no significant difference compared to the control (p > 0.05). Er exhibed the lowest inflammation scores after seven days of treatment (p < 0.05). PvI showed reduction of microorganisms at both times and under aerobic (p < 0.01 at 3 days and p < 0.001 at 7 days) and microaerophilic (p < 0.05) conditions. Er significantly reduced the count of microorganisms in aerobic condition when compared to control group (p < 0.05 at 3 days and p < 0.01 at 7 days). Conclusions All drugs promoted reduction of the microorganisms at the site of the injury, which may have a direct effect on the tissue repair process.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2018.01.001
      Issue No: Vol. 87 (2018)
    • Authors: Elerson Gaetti-Jardim; Ellen Cristina Gaetti Jardim; Christiane Marie Schweitzer; Júlio Cesar Leite da Silva; Murilo Moura Oliveira; Danilo Chizzolini Masocatto; Cauê Monteiro dos Santos
      Abstract: Publication date: Available online 8 January 2018
      Source:Archives of Oral Biology
      Author(s): Elerson Gaetti-Jardim, Ellen Cristina Gaetti Jardim, Christiane Marie Schweitzer, Júlio Cesar Leite da Silva, Murilo Moura Oliveira, Danilo Chizzolini Masocatto, Cauê Monteiro dos Santos
      Objective This case-control study aimed to evaluate the effects of conventional radiotherapy (RT) on the prevalence and populations of oral microorganisms in head and neck cancer patients who did not receive adequate preventive dental care. It was hypothesized that side effects of radiotherapy could be associated with radiation dose, microbiological aspects, and socioeconomic conditions of the patients. Design Twenty-eight dentate patients with head and neck cancer submitted to RT were included in the study. Radiation dose received varied from 4320 to 7020 cGy. Patients with the same demographic and health conditions, but no history of cancer or antineoplastic treatment were used as controls. Clinical examinations were carried out before RT, 15–22 days after starting RT, immediately after and 6 months after RT. Supra and subgingival biofilms were collected and cultivated onto selective and non-selective media. Isolates were identified by biochemical and physiological characteristics. Stimulated and unstimulated salivary flow rate and saliva buffer capacity were also determined. Results Mucositis, dermatitis, xerostomia, dysgeusia, dysphagia and candidiasis were common after starting RT and during the treatment period. Xerostomia was followed by a decrease in salivary pH and buffer capacity, which showed association with the increase of cariogenic cocci and yeast populations, which were also associated with deterioration of hygiene. Candida and family Enterobacteriaceae showed increased prevalence with RT, and were associated with the occurrence of mucositis and xerostomia. Conclusions Modifications in oral biofilms of irradiated patients showed association with xerostomia and hygiene conditions, which reinforces the necessity of improving patient compliance to oral health care programs.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2018.01.003
  • An RNA-seq screen of P. gingivalis LPS treated human gingival fibroblasts
    • Authors: Yufeng Xie; Mengjun Sun; Yiru Xia; Rong Shu
      Abstract: Publication date: Available online 8 January 2018
      Source:Archives of Oral Biology
      Author(s): Yufeng Xie, Mengjun Sun, Yiru Xia, Rong Shu
      Background and objective: In gingival tissues, lipopolysaccharide (LPS) from Porphyromonas gingivalis (P. gingivalis) is the most critical stimulator for inducing inflammatory response. Human gingival fibroblasts (HGFs) are the major constituents of gingival connective tissues. The aim of this study was to investigate P. gingivalis LPS induced whole transcriptional profile in HGFs and the potential crosstalk between microRNAs (miRNAs) and inflammatory cytokines. Methods RNA-seq was performed on HGFs with and without P. gingivalis LPS treatment. The gene expression of selected inflammatory cytokines and miRNAs induced by LPS at different time points was evaluated by quantitative RT-PCR. The protein expression of chemokines was further confirmed by ELISA. Results Interestingly, most of the significantly changed genes (198/204) were up-regulated at 4 h after 10 μg/ml LPS stimulation, including inflammatory cytokines and miRNAs. Confirmed by quantitative RT-PCR, the mRNA levels of IL-1β, IL-6 and IL-8 showed single up-regulation peak (4 h/6 h) after 1 μg/ml and 10 μg/ml LPS treatment. Similarly, 1 μg/ml LPS induced single up-regulation peak (8 h) of miRNA-146a, −146b and −155 expression. However, 10 μg/ml LPS induced the increased expression of miRNA-146a and −155 at both early stage (2 h/4 h) and late stage (24 h). Conclusion Taken together, we investigated P. gingivalis LPS induced whole transcriptional profile, and the different behaviors of miRNA expression induced by different doses of LPS in HGFs.

      PubDate: 2018-01-09T22:52:43Z
      DOI: 10.1016/j.archoralbio.2018.01.002
  • Reliability of a new test food to assess masticatory function
    • Authors: Laura Khoury-Ribas; Raul Ayuso-Montero; Bernat Rovira-Lastra; Maria Peraire; Jordi Martinez-Gomis.
      Pages: 1 - 6
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Laura Khoury-Ribas, Raul Ayuso-Montero, Bernat Rovira-Lastra, Maria Peraire, Jordi Martinez-Gomis.
      Objective This study assessed the reliability and validity of masticatory function assessment using a new test food, Optozeta. Design Thirty-five adults participated in the cross-sectional clinical part of the study; ten of them performed a retest. They performed two free-style masticatory tests consisting of five trials of 20 cycles each chewing three pieces of Optosil or Optozeta placed in a latex bag. Optozeta was created by mixing 50% Optosil with 50% of Zetalabor. Masticatory performance, masticatory laterality and chewing rate were assessed. Reliability and construct validity were assessed using the intraclass correlation coefficient (ICC) and Spearman correlations, respectively. Results Higher ICC values were observed for each aspect of masticatory function as assessed using Optozeta compared with Optosil. All the participants showed a lower median particle size value using Optozeta than Optosil. For each masticatory parameter, a high correlation was observed between using Optosil or Optozeta. Conclusions Optozeta seems to have good construct validity and appears to be more reliable than Optosil as a test food to assess masticatory function.

      PubDate: 2017-12-12T19:05:36Z
      DOI: 10.1016/j.archoralbio.2017.12.006
      Issue No: Vol. 87 (2017)
  • When forensic odontology met biochemistry: Multidisciplinary approach in
           forensic human identification
    • Authors: Joe Adserias-Garriga; Christian Thomas; Douglas H. Ubelaker; Sara C. Zapico
      Pages: 7 - 14
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Joe Adserias-Garriga, Christian Thomas, Douglas H. Ubelaker, Sara C. Zapico
      When human remains are found, the priority of the investigation is to ascertain the identity of the deceased. A positive identification is a key factor in providing closure for the family of the deceased; it is also required to issue the death certificate and therefore, to settle legal affairs. Moreover, it is difficult for any forensic investigation involving human remains to be solved without the determination of an identity. Therefore, personal identification is necessary for social, legal and forensic reasons. In the last thirty years forensic odontology has experienced an important transformation, from primarily involving occasional dental identification into a broader role, contributing to the determination of the biological profile. In the same way, “DNA fingerprinting” has evolved not only in terms of improving its technology, but also in its application beyond the “classical”: helping with the estimation of sex, age and ancestry. As these two forensic disciplines have developed independently, their pathways have crossed several times through human identification operations, especially the ones that require a multidisciplinary approach. Thus, the aim of this review is to describe the contributions of both forensic odontology and molecular biology/biochemistry to human identification, demonstrating how a multidisciplinary approach can lead to a better and more efficient identification.

      PubDate: 2017-12-12T19:05:36Z
      DOI: 10.1016/j.archoralbio.2017.12.001
      Issue No: Vol. 87 (2017)
  • “Omics” in oral cancer: New approaches for biomarker discovery
    • Authors: Vertika Rai; Rashmi Mukherjee; Ananta K. Ghosh; Aurobinda Routray; Chandan Chakraborty
      Pages: 15 - 34
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Vertika Rai, Rashmi Mukherjee, Ananta K. Ghosh, Aurobinda Routray, Chandan Chakraborty
      Objectives In this review paper, we explored the application of “omics” approaches in the study of oral cancer (OC). It will provide a better understanding of how “omics” approaches may lead to novel biomarker molecules or molecular signatures with potential value in clinical practice. A future direction of “omics”-driven research in OC is also discussed. Methods Studies on “omics”-based approaches [genomics/proteomics/transcriptomics/metabolomics] were investigated for differentiating oral squamous cell carcinoma,oral sub-mucous fibrosis, oral leukoplakia, oral lichen planus, oral erythroplakia from normal cases. Electronic databases viz., PubMed, Springer, and Google Scholar were searched. Results One eighty-one studies were included in this review. The review shows that the fields of genomics, transcriptomics, proteomics, and metabolomics-based marker identification have implemented advanced tools to screen early changes in DNA, RNA, protein, and metabolite expression in OC population. Conclusions It may be concluded that despite advances in OC therapy, symptomatic presentation occurs at an advanced stage, where various curative treatment options become very limited. A molecular level study is essential for detecting an OC biomarker at an early stage. Modern “Omics” strategies can potentially make a major contribution to meet this need.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.003
      Issue No: Vol. 87 (2017)
  • Effects of Rhodomyrtus tomentosa extract on virulence factors of Candida
           albicans and human neutrophil function
    • Authors: Jutharat Hmoteh; Khadar Syed Musthafa; Supayang Piyawan Voravuthikunchai
      Pages: 35 - 42
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Jutharat Hmoteh, Khadar Syed Musthafa, Supayang Piyawan Voravuthikunchai
      Objective Candida albicans has become a major problem of oral candidiasis due to increase in antibiotic resistance. Rhodomyrtus tomentosa, a medicinal plant possessing several phytochemical constituents, has been considered as a potential source of antimicrobial and immunomodulatory agents. The aim of this study was to investigate anti-virulence and immunostimulatory activity of R. tomentosa ethanolic leaf extract against C. albicans. Methods The effects of the extract on C. albicans were assessed on germ tube production, adherence of the organisms to surfaces, and biofilm. In addition, the effects of the extract on phagocytosis and killing activity of neutrophils against the pathogen were investigated. Results Suppression of germ tube production following 30 min exposure to the extract at 256 μg/mL was significantly increased in comparison with that of the unexposed cells (p < 0.05). The pathogens demonstrated a significant reduction in adherence ability to surfaces in a dose dependent manner, compared with the control (p < 0.05). At 48 h, the extract at 512–1024 μg/mL significantly reduced biofilm forming ability of the organisms up to 42.31–64.58% (p < 0.05). Compared with the control group, a significant inhibition of mature biofilm after treated with the extract at 256 μg/mL was observed (p < 0.05). The extract at 50 μg/mL significantly enhanced phagocytosis and killing activity of neutrophils against the organism, compared with the control (p < 0.05). Conclusions The findings suggest that R. tomentosa extract displayed a dual mode of action, inhibiting virulence factors of C. albicans and enhancing neutrophil functions. Further pharmaceutical development of the extract might be useful as an alternative therapeutic agent against oral candidiasis.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.11.007
      Issue No: Vol. 87 (2017)
  • Matrix metalloproteinase-8 analysis in patients with periodontal disease
           with prediabetes or type 2 diabetes mellitus: A systematic review
    • Authors: Everton Freitas de de Morais; Afonso Nóbrega Dantas; Juliana Campos Pinheiro; Rafaella Bastos Leite; Carlos Augusto Galvao Barboza; Bruno Cesar de Vasconcelos Gurgel; Roseana de Almeida Freitas
      Pages: 43 - 51
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Everton Freitas de de Morais, Afonso Nóbrega Dantas, Juliana Campos Pinheiro, Rafaella Bastos Leite, Carlos Augusto Galvao Barboza, Bruno Cesar de Vasconcelos Gurgel, Roseana de Almeida Freitas
      Objectives The objective of this systematic review was to evaluate information on the levels of MMP-8 in patients diagnosed with prediabetes or type 2 diabetes mellitus with periodontal disease, analyzing its validity as a possible biomarker for the diagnosis and progression of periodontal disease (PD). Methods A systematic search of the following databases was performed: PubMed/Medline, CENTRAL (The Cochrane Library), EMBASE and Web of Science. Studies involving the evaluation of MMP-8 in patients with prediabetes or patients presenting type 2 diabetes mellitus concomitantly with PD were selected. The evaluation of the methodological quality of the selected studies was based on the methodological bias risk analysis (QUADAS-2). Results Eight of the initially identified 2683 articles were selected. In all the selected studies, evaluator calibration and the use of clear methods for patient diagnosis with periodontal disease were present. Studies have demonstrated significantly higher MMP-8 concentrations in PD patients compared to controls, as well as in patients presenting more advanced stages of PD. However, controversies regarding MMP-8 levels in prediabetes/diabetes type 2 patients with PD. Conclusions Higher MMP-8 levels in patients with PD compared to controls imply the potential use of MMP-8 in the diagnosis of PD. The influence of patient glycemic state, as well as medications these patients make use of, are factors that possibly contribute to the modulation of MMP-8 concentrations in patients with diabetes and should be analyzed, aiming at a better understanding of the relationship between glycemic state and MMP-8 levels in patients with PD.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.008
      Issue No: Vol. 87 (2017)
  • The WNT7B protein promotes the migration and differentiation of human
           dental pulp cells partly through WNT/beta-catenin and c-Jun N-terminal
           kinase signalling pathways
    • Authors: Hongyang Lv; Jing Yang; Chenglin Wang; Fanyuan Yu; Dingming Huang; Ling Ye
      Pages: 54 - 61
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Hongyang Lv, Jing Yang, Chenglin Wang, Fanyuan Yu, Dingming Huang, Ling Ye
      Objective The aim of this study is to investigate the role of the WNT7B protein in the migration and differentiation of human dental pulp cells (HDPCs). Design The effect of recombinant human WNT7B (rhWNT7B) on the proliferation and migration of HDPCs was evaluated by 5-ethynyl-2′-deoxyuridine (EdU), immunofluorescence staining of Ki67, flow cytometry and scratch assay; the differentiation of HDPCs was measured by alkaline phosphatase (ALP) staining, alizarin red staining, ALP activity, qPCR and western blot. The activation of the WNT/beta-catenin (WNT/β-catenin) and c-Jun N-terminal kinase (JNK) pathways was analysed by western blot, immunocytochemistry and dual luciferase assays. XAV939 and SP600125,the inhibitors of the WNT/β-catenin and JNK pathways, were further applied to verify the mechanism. Results rhWNT7B repressed the proliferation but did not affect the apoptosis of HDPCs. In the presence of rhWNT7B, ALP and alizarin red staining were increased substantially in the HDPCs with osteogenic induction; the gene expression of Runx2 and Col1 in HDPCs was quite elevated compared with that induced in osteogenic medium without WNT7B measured by qPCR; The ALP activity was also increased with rhWNT7B stimulation in HDPCs after 7-day odontogenic culture; Western blot revealed that the expression of dentin sialophosphoprotein (DSPP) of HDPCs was up-regulated significantly with the addition of WNT7B as well. Further study showed that rhWNT7B activated the WNT/β-catenin and JNK signalling pathways in the differentiation of HDPCs. XAV939 and SP600125 can partly offset the effect of the WNT7B-induced differentiation of HDPCs. Conclusion WNT7B promoted the differentiation of HDPCs partly through the WNT/β-catenin and JNK signalling pathways.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.015
      Issue No: Vol. 87 (2017)
  • EphrinB2 signaling enhances osteogenic/odontogenic differentiation of
           human dental pulp stem cells
    • Authors: Boon Chin Heng; Shuai Wang; Ting Gong; Jianguang Xu; Changyong Yuan; Chengfei Zhang
      Pages: 62 - 71
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Boon Chin Heng, Shuai Wang, Ting Gong, Jianguang Xu, Changyong Yuan, Chengfei Zhang
      Objective To investigate the role of the EphrinB2 signaling pathway in the osteogenesis/odontogenesis of human dental pulp stem cells (DPSCs). Design The endogenous expression levels of EphrinB2 and its cognate receptors EphB2 and EphB4 in DPSCs were analyzed by qRT-PCR and Western blotting after 7, 14 and 21 days of osteogenic/odontogenic induction culture. Additionally, the phosphorylation of EphrinB2, EphB4 and ERK1/2 proteins at early time-points following osteogenic induction, were also investigated by Western blots. Subsequently, we investigated whether supplementation of recombinant EphrinB2-Fc within the induction milieu can enhance the osteogenic/odontogenic differentiation of DPSCs. Results Endogenous gene and protein expression levels of EphrinB2, EphB2 and EphB4 were upregulated in induced versus non-induced DPSCs, over 21 days of osteogenic/odontogenic induction. Western blots showed increase in phosphorylated EphrinB2, EphB4 and ERK1/2 proteins at early time-points following osteogenic induction. Preliminary investigation of a concentration range (0, 0.5, 1 and 2 μg/ml) of recombinant EphrinB2-Fc within osteogenic induction media, showed that 0.5 μg/ml was optimal for enhancing the osteogenic/odontogenic differentiation of DPSCs over a culture duration of 14 days. Subsequently, more comprehensive qRT-PCR analysis with 0.5 μg/ml EphrinB2-Fc revealed significant upregulation of several key osteogenic marker genes in treated versus untreated DPSCs after 21 days of osteogenic/odontogenic induction. By 7 days of osteogenic induction, DPSCs treated with 0.5 μg/ml EphrinB2-Fc exhibited significantly more calcium mineralization (Alizarin red S staining) and alkaline phosphatase activity than the untreated control. Conclusions EphrinB2 signaling plays a key role in the osteogenic/odontogenic differentiation of DPSCs.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.014
      Issue No: Vol. 87 (2017)
  • Age-related changes of CD4+ T cell migration and cytokine expression in
           germ-free and SPF mice periodontium
    • Authors: Koichiro Irie; Takaaki Tomofuji; Daisuke Ekuni; Daiki Fukuhara; Yoko Uchida; Kota Kataoka; Shuichiro Kobayashi; Takeshi Kikuchi; Akio Mitani; Yoshihiro Shimazaki; Manabu Morita
      Pages: 72 - 78
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita
      Objective Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P < 0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P < 0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.007
      Issue No: Vol. 87 (2017)
  • Anti-inflammatory activity of cannabinoid receptor 2 ligands in primary
           hPDL fibroblasts
    • Authors: Ammaar H. Abidi; Chaela S. Presley; Mustafa Dabbous; David A. Tipton; Suni M. Mustafa; Bob M. Moore
      Pages: 79 - 85
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Ammaar H. Abidi, Chaela S. Presley, Mustafa Dabbous, David A. Tipton, Suni M. Mustafa, Bob M. Moore
      Objectives Approximately 65 million adults in the US have periodontitis, causing tooth loss and decreased quality of life. Cannabinoids modulate immune responses, and endocannabinoids are prevalent during oral cavity inflammation. Targets for intervention in periodontal inflammation are cannabinoid type 1 and 2 receptors (CB1R, CB2R), particularly CB2R because its levels increase during inflammation. We previously demonstrated that SMM-189 (CB2R inverse agonist) decreased pro-inflammatory cytokine production in primary microglial cells. The hypothesis of this study was that cannabinoids anandamide (AEA), HU-308 (CB2R selective agonist), and SMM-189 decrease pro-inflammatory IL-6 and MCP-1 production by primary human periodontal ligament fibroblasts (hPDLFs) stimulated with P. gingivalis LPS, TNF-α, or IL-1β. Design Cytotoxic effects of cannabinoid compounds (10−4–10−6.5 M), LPS (1–1000 ng/ml), TNFα (10 ng/ml) and IL-1β (1 ng/ml) were assessed by measuring effects on cellular dehydrogenase activity. IL-6 and MCP-1 production were measured using Mesoscale Discovery (MSD) Human Pro-Inflammatory IL-6 and MSD Human Chemokine MCP-1 kits and analyzed using MSD Sector 2400 machine. Results EC50 values for AEA, SMM-189, and HU-308 were 16 μM, 13 μM, and 7.3 μM respectively. LPS (1 μg/ml), TNF-α (10 ng/ml), and IL-1β (1 ng/ml) increased IL-6 and MCP-1 production, which were inhibited by AEA, SMM-189, and HU-308. AEA alone significantly increased IL-6, but not MCP-1 levels, but the other cannabinoids alone had no effect. Conclusion The effective inhibition of LPS, TNF-α, IL-1β stimulated IL-6 and MCP-1 production by CB2R ligands in hPDLFs suggests that targeting the endocannabinoid system may lead to development of novel drugs for periodontal therapy, aiding strategies to improve oral health.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.005
      Issue No: Vol. 87 (2017)
  • Inhibition of the receptor for advanced glycation promotes proliferation
           and repair of human periodontal ligament fibroblasts in response to high
           glucose via the NF-κB signaling pathway
    • Authors: Danting Zhan; Ling Guo; Lige Zheng
      Pages: 86 - 93
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Danting Zhan, Ling Guo, Lige Zheng
      Objective To observe if inhibition of the receptor for advanced glycation endproducts (RAGE) promotes proliferation and repair of human periodontal ligament fibroblasts (hPDLFs) stimulated by high glucose. In addition, we also discuss the effects of the NF-κB signaling pathway in relation to this process. Methods Primary cultured hPDLFs were exposed to either low glucose (5.5 mmol/L) or high glucose (25 mmol/L), and RAGE expression was measured by Western blot analysis. Cells were cultured in high glucose with different concentrations of the RAGE inhibitor, FPS-ZM1. We measured cell proliferation using the Cell Counting Kit-8 and expression of collagen type 1 and fibronectin by real-time PCR and ELISA, respectively. The relative protein expression levels of NF-κB p65 and phosphorylated p65 were measured by Western blot analysis. Results High glucose enhanced RAGE expression and suppressed cell growth. While FPS-ZM1 increased proliferation and expression of repair-related factors in high glucose, there was a concurrent decline in the phosphorylation level of NF-κB p65. Conclusion FPS-ZM1 rescued the proliferative capacity and repair capability of hPDLFs via the RAGE-NF-κB signaling pathway in response to high glucose.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.011
      Issue No: Vol. 87 (2017)
  • Three-dimensional growth pattern of the rat mandible revealed by periodic
           live micro-computed tomography
    • Authors: Hak-Jin Kim; Kyeong-Mee Park; Hye-Jin Tak; Ji Wook Choi; Sang-Hoon Kang; Wonse Park; Sang-Hwy Lee
      Pages: 94 - 101
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Hak-Jin Kim, Kyeong-Mee Park, Hye-Jin Tak, Ji Wook Choi, Sang-Hoon Kang, Wonse Park, Sang-Hwy Lee
      Objective We wanted to evaluate the three-dimensional (3D) mandibular growth of Sprague-Dawley rats from 4th to 16th postnatal weeks with periodic and live micro-computed tomographic scanning. Design Twenty Sprague-Dawley rats were used for micro-CT scanning from 4th to 16th postnatal weeks. After 3D reconstruction of rat mandible, we performed the linear and angular measurements and the superimposition of the 3D models to evaluate the mandibular growth of rat. Results The results showed that the growth direction of the condylar and coronoid regions was superior primarily and posterior secondarily, while the condyle had minimal lateral growth. Moreover, the angular region was growing mainly toward the posterior and lateral direction, while the body and symphysis maintained small, incremental anterior-posterior growth. Conclusions We could evaluate the amount, rate, and direction of growth using the mandibular skeletal unit. Some reference points and measurements were more relevant in properly characterizing 3D growth of the mandible. Their growth rates were the greatest between 4th and 8th postnatal weeks, a period which seems most appropriate for studies of rat mandible growth.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.012
      Issue No: Vol. 87 (2017)
  • Mechanical stiffness of TMJ condylar cartilage increases after artificial
           aging by ribose
    • Authors: Fereshteh Mirahmadi; Jan Harm Koolstra; Frank Lobbezoo; G.Harry van Lenthe; Samaneh Ghazanfari; Jessica Snabel; Reinout Stoop; Vincent Everts
      Pages: 102 - 109
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Fereshteh Mirahmadi, Jan Harm Koolstra, Frank Lobbezoo, G.Harry van Lenthe, Samaneh Ghazanfari, Jessica Snabel, Reinout Stoop, Vincent Everts
      Objective Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation. Methods Porcine TMJ condyles were incubated for 7 days with different concentrations of ribose. The compressive modulus and stiffness ratio (incubated versus control) was determined after loading. Glycosaminoglycan and collagen content, and the number of crosslinks were analyzed. Tissue structure was visualized by microscopy using different staining methods. Results Concomitant with an increasing concentration of ribose, an increase of collagen crosslinks was found. The number of crosslinks increased almost 50 fold after incubation with the highest concentration of ribose. Simultaneously, the stiffness ratio of the samples showed a significant increase after incubation with the ribose. Pearson correlation analyses showed a significant positive correlation between the overall stiffness ratio and the crosslink level; the higher the number of crosslinks the higher the stiffness. Conclusion The present model, in which ribose was used to mimic certain aspects of age-related changes, can be employed as an in vitro model to study age-related mechanical changes in the TMJ condyle.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.010
      Issue No: Vol. 87 (2017)
  • Prediction of agenesis of the mandibular second premolar using the
           developmental stages of the mandibular canine, first premolar, and second
    • Authors: Ji-Soo Song; Teo Jeon Shin; Young-Jae Kim; Jung-Wook Kim; Ki-Taeg Jang; Sang-Hoon Lee; Hong-Keun Hyun
      Pages: 110 - 114
      Abstract: Publication date: March 2018
      Source:Archives of Oral Biology, Volume 87
      Author(s): Ji-Soo Song, Teo Jeon Shin, Young-Jae Kim, Jung-Wook Kim, Ki-Taeg Jang, Sang-Hoon Lee, Hong-Keun Hyun
      Objective The aim in this study was to suggest a standard for early diagnosis of agenesis of the mandibular second premolars (L5) by estimating the developmental stages of the mandibular canines (L3), first premolars (L4), and second molars (L7). Design Among all 5136 pediatric patients aged 5–11 years who received panoramic radiographs between June 2008 and December 2009 at Seoul National University Dental Hospital, 215 control patients and 74 agenesis patients who met inclusion criteria were analyzed. The developmental stages of all L3, L4, L5, and L7 of control and agenesis patients were estimated using the method proposed by Demirjian. To identify the tooth (L3, L4, L7) with the developmental pattern most similar to that of L5, Kendall rank correlation coefficients and Bootstrap method were used. To verify that patients with agenesis of L5 show delayed development, Wilcoxon rank sum test was used. To identify the stages in which to diagnose agenesis of L5, we performed survival analysis. Results There was a significant correlation between the developmental stages of L3, L4, L7 and L5. The developmental stages of those three teeth in the agenesis group were delayed compared with those in the control group at certain ages. If the developmental stages of at least two of those three teeth reach Demirjian stage D without the calcification of L5, agenesis of L5 can be confirmed. Conclusions Agenesis of L5 can be confirmed when two of the three teeth (L3, L4, L7) reach Demirjian stage D.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.12.018
      Issue No: Vol. 87 (2017)
  • Secretoneurin and PE-11 immunoreactivity in the human dental pulp
    • Authors: René Steiner; Reiner Fischer-Colbrie; Athanasia Bletsa; Johannes Laimer; Josef Troger
      Pages: 13 - 17
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): René Steiner, Reiner Fischer-Colbrie, Athanasia Bletsa, Johannes Laimer, Josef Troger
      Objective To explore whether there are differences in the concentration of the secretogranin II-derived peptide secretoneurin and the chromogranin B-derived peptide PE-11 between the healthy and inflamed human dental pulps. Furthermore, colocalization studies with calcitonin gene-related peptide were performed to confirm the sensory origin of the peptidergic nerves in the dental pulp. Design The concentrations of secretoneurin and PE-11 were determined by highly sensitive radioimmunoassays in extracts of dental pulps, the molecular form of secretoneurin immunoreactivities by RP-HPLC with subsequent radioimmunoassay and colocalization studies with calcitonin gene-related peptide were performed by double immunofluorescence. Results Only secretoneurin but not PE-11 was detectable by radioimmunoassays whereas nerve fibers could be made visible for both secretoneurin and PE-11. Furthermore, there was a full colocalization of secretoneurin and PE-11 with calcitonin gene-related peptide in immunohistochemical experiments. There were no differences in the concentration of secretoneurin between the healthy and inflamed human dental pulp and moreover, the characterization of the secretoneurin immunoreactivities revealed that only authentic secretoneurin was detected with the secretoneurin antibody. Conclusions There is unequivocal evidence that secretoneurin and PE-11 are constituents of the sensory innervation of the human dental pulp and although not exclusively but are yet present in unmyelinated C-fibers which transmit predominantly nociceptive impulses. Secretoneurin might be involved in local effector functions as well, particularly in neurogenic inflammation, given that this is the case despite of unaltered levels in inflamed tissue.

      PubDate: 2017-11-15T16:18:10Z
      DOI: 10.1016/j.archoralbio.2017.11.005
      Issue No: Vol. 86 (2017)
  • The risk factors related to bruxism in children: A systematic review and
    • Authors: Huaqi Guo; Tongxia Wang; Xiaohong Niu; Hui Wang; Weihan Yang; Jie Qiu; Lan Yang
      Pages: 18 - 34
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Huaqi Guo, Tongxia Wang, Xiaohong Niu, Hui Wang, Weihan Yang, Jie Qiu, Lan Yang
      Objective This systematic review was performed to determine the risk factors related to bruxism in children. Design This systematic review was conducted with reporting in agreement to the PRISMA statement and according to guidelines from the Cochrane Handbook for Systematic Reviews of Interventions. We conducted a systematic search of seven online databases, with the last search updated on 1st October 2016. The seven databases were Pubmed, Embase, Cochrane Library database, Web of Science, CNKI, CBM, and WF. The included trial type were RCT, cohort studies, and case-control studies, and bruxism symptoms were assessed by questionnaires and examinations. Eighteen out of the 5637 initially identified studies met the inclusion and exclusion criteria. Results gender, age, gene, mixed position, anxiety, the nervous, secondhand smoke, high psychological reactions, responsibility, move a lot during sleep, sleeps with mouth open, snores loudly, restless sleep, sleep hours, sleep with light on, noise in room, headache, biting, cheeks tonus, perioral musculature participation, conduct problems, peer problems, emotional symptoms, mental health problems, birth weight, occupation of family head, maternal marital status, hyperactivity, family income seemed to have statistical significance from the present systematic review and meta-analysis. Conclusions The risk factors related to bruxism were as follows: Male, gene, mixed position, moves a lot, anxiety, the nervous, psychological reactions, responsibility, secondhand smoke, snore loudly, restless sleep, sleep with light on, noise in room, “sleep hours, ≤8h”, headache, objects biting, conduct problems, peer problems, emotional symptoms and mental health problems.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.11.004
      Issue No: Vol. 86 (2017)
  • The chemokine receptor type 4 antagonist, AMD3100, interrupts experimental
           tooth movement in rats
    • Authors: Kasumi Hatano; Yuji Ishida; Hiroyuki Yamaguchi; Jun Hosomichi; Jun-ichi Suzuki; Risa Usumi-Fujita; Yasuhiro Shimizu; Naoki Shibutani; Sawa Kaneko; Takashi Ono
      Pages: 35 - 39
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Kasumi Hatano, Yuji Ishida, Hiroyuki Yamaguchi, Jun Hosomichi, Jun-ichi Suzuki, Risa Usumi-Fujita, Yasuhiro Shimizu, Naoki Shibutani, Sawa Kaneko, Takashi Ono
      Objective The aim of this study was to clarify the role of the stromal cell-derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4) axis in osteoclast accumulation, and the influence of orthodontic tooth movement (OTM) under mechanical force application to periodontal tissues, by administration of the CXCR4 antagonist AMD3100. Design The upper right first molar (M1) of rats was moved mesially with a 10-g force titanium-nickel closed coil spring. Rats were treated with phosphate-buffered saline or AMD3100 (5mg/kg), which is a SDF-1 antagonist. After 0, 1, 3, and 7days, alveolar bones in all groups were examined at each time point by micro-computed tomography and histological analysis. Results Tooth movement was decreased significantly in the AMD3100-treated group at 1, 3, and 7days after beginning OTM. The numbers of tartrate-resistant acid phosphatase-positive multinucleated cells in the periodontal ligament around the maxillary M1 were decreased significantly in the treated as compared to the control group on Days 1 and 3. Conclusion Administration of AMD3100 decreases OTM and osteoclast accumulation in rat molars under orthodontic force application. These findings suggest that the SDF-1/CXCR4 axis plays an important role in alveolar bone metabolism during OTM.

      PubDate: 2017-11-15T16:18:10Z
      DOI: 10.1016/j.archoralbio.2017.11.003
      Issue No: Vol. 86 (2017)
  • Effect of laser activated bleaching on the chemical stability and
           morphology of intracoronal dentin
    • Authors: Fabiane Carneiro Lopes; Renato Roperto; Anna Akkus; Ozan Akkus; Regina Guenka Palma-Dibb; Manoel Damião de Sousa-Neto
      Pages: 40 - 45
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Fabiane Carneiro Lopes, Renato Roperto, Anna Akkus, Ozan Akkus, Regina Guenka Palma-Dibb, Manoel Damião de Sousa-Neto
      Objectives To evaluate the effect of the bleaching with 35% hydrogen peroxide either activated or not by a 970nm diode laser on the chemical stability and dentin surface morphology of intracoronary dentin. Methods Twenty-seven slabs of intracoronary dentin specimens (3×3mm) were distributed into three groups (n=9), according to surface treatment: HP – 35% hydrogen peroxide (1×4’), DL – 970nm diode laser (1×30”/0,8W/10Hz), HP+DL – 35% HP activated with 970nm diode laser (1×30”/0,8W/10Hz leaving the gel in contact to the surface for 4′ after activation). Three Raman spectra from each fragment were obtained to calculate the mean intensity of peaks of inorganic component (a.u.), organic collagen content (a.u.), and the ratio of inorganic/organic content, before and after treatment. Analyses of the samples by confocal laser microscopy were performed to evaluate the surface roughness, percentage of tubules, perimeter and area percentage of tubules, before and after treatment. Data were analyzed by Kruskal-Wallis, Dunn’s, and Wilcoxon test (P <0.05). Results Data analysis showed that HP+DL did not change the inorganic content peaks 8.31 [29.78] or the inorganic/organic ratio 3.37 [14.67] (P> 0.05). Similarly, DL did not affect the chemical stability of the dentin surface (P> 0.05). However, HP significantly increased inorganic content peaks 10.87 [22.62], as well as the inorganic/organic ratio 6.25 [27.78] (P< 0.05). Regarding the morphological alterations, all surface treatments increase tubules exposure; HP treatment significantly increases perimeter and area percentage; and HP+DL increases surface roughness. Conclusions Bleaching HP combined with DL offers an improvement in terms of intracoronal dentin surface protection, yielding better maintenance of dentin chemical stability and morphology.

      PubDate: 2017-11-26T23:01:06Z
      DOI: 10.1016/j.archoralbio.2017.10.023
      Issue No: Vol. 86 (2017)
  • The effect of magnesium hydroxide-containing dentifrice using an extrinsic
           and intrinsic erosion cycling model
    • Authors: Vanara Florêncio Passos; Lidiany Karla Azevedo Rodrigues; Sérgio Lima Santiago
      Pages: 46 - 50
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Vanara Florêncio Passos, Lidiany Karla Azevedo Rodrigues, Sérgio Lima Santiago
      Objective To evaluate, in vitro, the effect of Mg(OH)2 dentifrice, and the influence of the number of experimental days, on the extrinsic (citric acid –CA) and intrinsic (hydrochloric acid –HCl) enamel erosion models. Design Human enamel slabs were selected according to surface hardness and randomly assigned to 3 groups (n=9) as follows: non-fluoridated (negative control), NaF (1450ppm F- positive control) and Mg(OH)2 (2%) dentifrices. The slabs were daily submitted to a 2-h period of pellicle formation and, over a period of 5days, submitted to cycles (3×/day) of erosive challenge (CA 0.05M, pH=3.75 or HCl 0.01M, pH=2 for 30s), treatment (1min −1:3w/w of dentifrice/distilled water) and remineralization (artificial saliva/120min). Enamel changes were determined by surface hardness loss (SHL) for each day and mechanical profilometry analysis. Data were analyzed by two-way ANOVA followed by Tukey’s test to % SHL and one-way ANOVA to profilometry (p<0.05). Results The number of experimental days influenced the erosion process for the two types of erosion models (p<0.001). Mg(OH)2-containing dentifrices were effective in reducing enamel extrinsic acid erosion as determined by % SHL (p<0.001) when compared to the control group, being better than positive control (p<0.001); however, the dentifrices were not effective for the intrinsic model (p=0.295). With regards to surface wear, no statistically significant differences were found among the groups for CA (p=0.225) and HCl (p=0.526). Conclusion The findings suggest that Mg(OH)2 dentifrices might protect enamel against slight erosion, but protection was not effective for stronger acid erosion.

      PubDate: 2017-11-26T23:01:06Z
      DOI: 10.1016/j.archoralbio.2017.11.006
      Issue No: Vol. 86 (2017)
  • Influence of dentin pretreatment with 2.5% titanium tetrafluoride on
           inhibiting caries at the tooth-restoration interface in situ
    • Authors: Enrico Coser Bridi; Flávia Lucisano Botelho do Amaral; Fabiana Mantovani Gomes França; Cecilia Pedroso Turssi; Roberta Tarkany Basting
      Pages: 51 - 57
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Enrico Coser Bridi, Flávia Lucisano Botelho do Amaral, Fabiana Mantovani Gomes França, Cecilia Pedroso Turssi, Roberta Tarkany Basting
      Objective Investigate the effects of dentin pretreatment with 2.5% titanium tetrafluoride (TiF4) aqueous solution followed by two-step self-etching (CLE/Clearfil SE Bond) and one-step self-etching adhesive systems (SBU/Single Bond Universal) on carious lesion inhibition at the tooth-restoration interface using an in situ model. Design Sixty-four cavities at the enamel-dentin junction of dental fragments were randomly distributed according to groups (n=16): 1) TiF4 +CLE; 2) TiF4 +SBU; 3) CLE; 4) SBU. Cavities were restored using resin composite, and placed in intraoral palatal devices used by 16 volunteers for 21days, to induce caries formation in situ. The fragments were then ground-flat to perform Knoop microhardness tests. Nine indentations were performed on each enamel and dentin substrate, subjacent to the restoration. Analysis of variance and Tukey’s test were applied. Results Enamel: groups receiving TiF4 dentin pretreatment (regardless of adhesive system and tooth-restoration interface distance) presented higher hardness means at a depth of 25μm from the outer tooth surface (p<0.0001). Dentin: groups receiving CLE presented higher means when applying TiF4 pretreatment, whereas groups restored with SBU presented higher means without pretreatment (p=0.0003). Conclusions Dentin pretreatment with TiF4 inhibited demineralization of the enamel interface in situ, regardless of the adhesive, and TiF4 pretreatment followed by CLE application showed higher potential for inhibiting dentin demineralization at the interface.

      PubDate: 2017-11-26T23:01:06Z
      DOI: 10.1016/j.archoralbio.2017.10.021
      Issue No: Vol. 86 (2017)
  • The use of laser Doppler flowmetry to evaluate oral soft tissue blood flow
           in humans: A review
    • Authors: Ayepa Alain Kouadio; Fabienne Jordana; N’goran Justin Koffi; Pierre Le Bars; Assem Soueidan
      Pages: 58 - 71
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Ayepa Alain Kouadio, Fabienne Jordana, N’goran Justin Koffi, Pierre Le Bars, Assem Soueidan
      The objective of this work is to define the conditions for improving the use of laser Doppler flowmetry (LDF) and to determine the limits for the use of this technique. This article systematically reviews the literature on the evaluation of oral soft tissue blood microcirculation by LDF. We analysed the available literature through October 2016 using the database resources Medline/PubMed, the Cochrane Oral Health Group Specialist Trials Register and the ISI Web of Knowledge. Several points emerged from this literature review The use of LDF involves specific constraints; however, the influence of different factors (temperature, tobacco, pressure etc.) must be adequately controlled when using LDF. LDF measurements of soft tissue within the oral cavity vary depending on the anatomical site. In dentistry, LDF can be used to track healing progress in periodontal surgery and to diagnose vascular flow changes in the connective tissue of mucosae covered by a removable prosthesis at an early stage prior to the onset of clinical inflammation signs.

      PubDate: 2017-12-24T22:01:54Z
      DOI: 10.1016/j.archoralbio.2017.11.009
      Issue No: Vol. 86 (2017)
  • Aspects of the final phase of enamel formation as evidenced by
           observations of superficial enamel of human third molars using scanning
           electron microscopy
    • Authors: Steinar Risnes; Chunfang Li
      Pages: 72 - 79
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Steinar Risnes, Chunfang Li
      Objective Enamel structure reflects ameloblast function. By studying the structure of the superficial enamel, information about ameloblast function toward the end of the secretory stage may be obtained. Design The superficial enamel in midcoronal areas of acid-etched facio-lingual sections from human third molars was studied in the scanning electron microscope (SEM). Results A great variation was observed in occurrence of prism-free enamel. Prism-free enamel dominated in 40% (mandibular) and 47% (maxillary) of observed areas and had a mean thickness of about 30μm. Striations in the prism-free enamel had an interstriae distance of about 3.3–3.8μm. The angle between prisms and enamel surface was about 60°, between prisms and Retzius lines about 45° and between Retzius lines and enamel surface about 15°. The distances between regularly occurring Retzius lines and between striations in the prism-free enamel both tended to decrease toward the enamel surface. Prisms could change direction as they approached the enamel surface, mostly in cervical direction. Where Retzius lines curved and converged occlusally, prisms tended to deviate in an occlusal direction. Conclusions Judged from the incremental lines and occurrence of prism-free enamel, ameloblasts slow down and tend to lose their Tomes’ process as they approach the end of secretion. The crystals of prism-free enamel belong to the same system as the interprism crystals of prismatic enamel. A method, based on the disposition of fine incremental lines, is suggested for evaluation of ameloblast dynamics in the last stage of enamel secretion.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.11.008
      Issue No: Vol. 86 (2017)
  • Impact of gingivitis treatment for diabetic patients on quality of life
           related to periodontal objective parameters: A randomized controlled
           clinical trial
    • Authors: Sheila C. Cortelli; Fernando O. Costa; Antonio Gargioni-Filho; Davi R. Aquino; Luis O.M. Cota; Alexandre P. Scherma; Taís B. Miranda; Jose R. Cortelli
      Pages: 80 - 86
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Sheila C. Cortelli, Fernando O. Costa, Antonio Gargioni-Filho, Davi R. Aquino, Luis O.M. Cota, Alexandre P. Scherma, Taís B. Miranda, Jose R. Cortelli
      Objectives Patients with diabetes have a poor oral health-related quality of life (OHRQoL). It is not clear if this situation could be changed with effective periodontal treatment. This study examined both patients with diabetes and systemically healthy individuals to discover the impact of a gingivitis treatment protocol on OHRQoL and its relation to objective periodontal parameters. Design After ultrasonic debridement, patients were randomly assigned to an essential-oils (EO) or placebo mouthwash group. At baseline and 3 months, OHRQoL was assessed with the Oral Health and Quality of Life-United Kingdom questionnaire (OHQoL–UK) along with clinical, halitometric, microbiological and inflammatory objective parameters. The primary outcome was a change in OHQoL–UK scores. A factor analysis was performed and the impact of the extracted quality of life factor (QLF) and its interactions with diabetes, treatment, and time on the objective parameters, were tested by multiple linear regression models (p < 0.05). Chi-Square test compared questionnaire-answering profiles (p<0.05). Results Combined treatment with EO provided OHQoL improvements in both systemic conditions. Positive effect of oral health status on quality of life increased in EO groups but not in placebo groups. Question I (self-confidence) showed the greatest factorial weight, while Question A (food intake) showed the lowest factorial weight. All patients who showed OHRQoL improvements and used the EO rinse showed the lowest plaque and gingival indices and lower levels of bacteria and volatile sulfur compounds. Conclusions OHRQoL positively changed overtime. Most effective treatment protocols would provide better improvements in OHRQoL which is related to periodontal objective measures.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.11.010
      Issue No: Vol. 86 (2017)
  • Quantitative sensory response of the SCM muscle on sustained low level
           activation simulating co-contractions during bruxing
    • Authors: Lydia Eberhard; Sophia Terebesi; Nikolaos Nikitas Giannakopoulos; Daniel Hellmann; Hans-Jürgen Schindler; Marc Schmitter; Doreen Pfau
      Pages: 87 - 93
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Lydia Eberhard, Sophia Terebesi, Nikolaos Nikitas Giannakopoulos, Daniel Hellmann, Hans-Jürgen Schindler, Marc Schmitter, Doreen Pfau
      Objective Bruxism is discussed as an etiological factor in the pathogenesis of orofacial and cervical pain. As the sternocleidomastoid muscle (SCM) is co-activated during clenching, our aim was to investigate, whether the muscle loading leads to peripheral or central sensitizations. Design In twenty-one healthy female volunteers, somatosensory profiles of the SCM were recorded according to the test battery of the German Research Network on Neuropathic Pain (DFNS) prior to and after an isometric muscle exercise. QST comprised thermal and mechanical stimuli. A submaximal activation of the SCM (15% MVC) was kept for 10min in sitting position. In separate test sessions one month apart, one sham and one verum experiment were conducted in randomized order. During the muscle loading, the parameters cold detection threshold (CDT), mechanical pain sensitivity (MPS) and pressure pain treshold (PPT) were tested and experimental pain recorded by visual analogoue scales (VAS). All test sessions were performed during the follicular phase of the menstrual cycle (day 5), to avoid effects on pain perception. Data were analyzed with Repeated Measures ANOVA (SPSS 22.0) Results No significant changes were found during or after (sham) loading except for stimulus-response-function (SR, P=0.01) and PPT (P=0.02) in the sham test. No effect was observed in the verum experiment (P=0.12 up to 1.0). Conclusion Prolonged low level contraction of the SCM does not evoke painful sensitization. In contrast, submaximal muscle activation seems to have a protective effect corresponding to a training effect preventing sensitization.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.09.030
      Issue No: Vol. 86 (2017)
  • Dickkopf-1 may regulate bone coupling by attenuating wnt/β-catenin
           signaling in chronic apical periodontitis
    • Authors: Xuelian Tan; Dingming Huang; Wei Zhou; Li Yan; Junli Yue; WanLu Lu; Dongzhe Song; Xuedong Zhou; Ling Ye; Lan Zhang
      Pages: 94 - 100
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Xuelian Tan, Dingming Huang, Wei Zhou, Li Yan, Junli Yue, WanLu Lu, Dongzhe Song, Xuedong Zhou, Ling Ye, Lan Zhang
      Objective Alveolar bone loss is a common outcome of chronic apical periodontitis. In this study, we investigated the involvement of the Dickkopf-1-Wnt/β-catenin signaling pathway in the attenuation of osteogenic differentiation induced by Escherichia coli lipopolysaccharide, and we evaluated the use of Dickkopf-1 inhibitor and Dickkopf-1 recombinant protein to reverse bone loss in different phases of osteogenic differentiation. Methods MC3T3-E1 cells grown in osteogenic medium were treated with Escherichia coli lipopolysaccharide for 24h during osteogenic induction on days 0, 1, 7, 14 and 21. Dickkopf-1 siRNA was added on days 0 and 1, and Dickkopf-1 recombinant was added on days 7, 14, and 21. Quantitative real-time PCR, Western blotting and alkaline phosphatase activity assays were performed to measure osteogenic marker expression and Wnt/β-catenin signaling. A rat apical periodontitis model was used to further evaluate the function of Dickkopf-1 in relation to bone loss. Results MC3T3-E1 cells treated with Escherichia coli lipopolysaccharide showed decreased mRNA expression of osteogenic markers. Wnt/β-catenin signaling was also inhibited, and Dickkopf-1 showed corresponding variations as quantified by Western blotting. Using Dickkopf-1 inhibitor or Dickkopf-1 recombinant protein at different phases of osteogenic differentiation in vitro partially reversed the decrease in osteogenic marker expression. The rat apical periodontitis model indicated that the Dickkopf-1 inhibitor could restore bone loss in the periapical area in vivo. Conclusions Dickkopf-1 may play a key regulatory role in determining the outcome for bone in inflammatory environments, and modulating the Wnt/β-catenin signaling pathway via Dickkopf-1 inhibitor or recombinant protein may provide a potential therapeutic option to prevent bone destruction in endodontic disease.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.11.012
      Issue No: Vol. 86 (2017)
  • High-refined carbohydrate diet promotes detrimental effects on alveolar
           bone and femur microarchitecture
    • Authors: C.C. Montalvany-Antonucci; M.C. Zicker; S. Macari; T.S.F. Pereira; I.M.A. Diniz; I. Andrade; A.V.M. Ferreira; T.A. Silva
      Pages: 101 - 107
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): C.C. Montalvany-Antonucci, M.C. Zicker, S. Macari, T.S.F. Pereira, I.M.A. Diniz, I. Andrade, A.V.M. Ferreira, T.A. Silva
      The impact of high-refined carbohydrate (HC) diet on fat accumulation, adipokines secretion and systemic inflammation is well described. However, it remains unclear whether these processes affect bone remodeling. Objective To investigate the effects of HC diet in the alveolar bone and femur parameters. Methods BalbC mice were fed with conventional chow or HC diet for 12 weeks. After experimental time maxillae, femur, blood and white adipose tissue samples were collected. Results The animals feed with HC diet exhibited considerable increase of adiposity index and adipose tissue levels of TNF-α, IL-6, IL-10, IL-1β, TGF-β and leptin. Microtomography analysis of maxillary bone revealed horizontal alveolar bone loss and disruption of trabecular bone in mice feed with HC diet. These deleterious effects were correlated with a disturbance in bone cells and an augmented expression of Rankl/Opg ratio. Consistently, similar effects were observed in femurs, which also exhibited a reduction in bone maximum load and stiffness. Conclusion Our data indicates that HC diet consumption disrupts bone remodeling process, favoring bone loss. Underlying mechanisms relies on fat tissue accumulation and also in systemic and local inflammation.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.11.013
      Issue No: Vol. 86 (2017)
  • Live cell imaging reveals different modes of cytotoxic action of extracts
           derived from commonly used luting cements
    • Authors: Rita Trumpaitė-Vanagienė; Alina Čebatariūnienė; Virginijus Tunaitis; Alina Pūrienė; Augustas Pivoriūnas
      Pages: 108 - 115
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Rita Trumpaitė-Vanagienė, Alina Čebatariūnienė, Virginijus Tunaitis, Alina Pūrienė, Augustas Pivoriūnas
      Objective To compare cytotoxicity of extracts derived from commonly used luting cements: Hoffmann’s Zinc Phosphate (ZPC), GC Fuji Plus Resin Modified Glass Ionomer (RMGIC) and 3M ESPE RelyX Unicem Resin Cement (RC) on primary human gingival fibroblasts (HGFs). Design HGFs were exposed to different concentrations of the ZPC, RMGIC and RC extracts. The cytotoxicity was assessed with the PrestoBlue Cell Viability Reagent and viable cells were counted by a haemocytometer using the trypan blue exclusion test. In order to determine the primary mechanism of the cell death induced by extracts from different luting cements, the real-time monitoring of caspase-3/-7 activity and membrane integrity of cells was employed. Results The extracts from the RMGIC and ZPC decreased the metabolic activity and numbers of viable cells. Unexpectedly, the extracts from the RC evoked only small effects on the metabolic activity of HGFs with a decreasing number of viable cells in a dose-and time-dependent manner. The live cell imaging revealed that the apoptosis was the primary mechanism of a cell death induced by the extracts derived from the RMGIC, whereas the extracts from the RC and ZPC induced a cell death through a necrotic and caspase-independent pathway. Conclusions The apoptosis was the primary mechanism of the cell death induced by the extracts derived from the RMGIC, whereas the extracts from the RC and ZPC induced a cell death via a necrotic pathway. We suggest that metabolic assays commonly used to assess the cytotoxicity of luting cements should be validated by alternative methods.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.11.011
      Issue No: Vol. 86 (2017)
  • Genomic integration and expression of the Aggregatibacter
           actinomycetemcomitans catalase gene in Aggregatibacter aphrophilus
    • Authors: Yuting Alice Yang; Ya-An Cheng; Casey Chen
      Pages: 116 - 122
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Yuting Alice Yang, Ya-An Cheng, Casey Chen
      Objective To test the hypothesis that virulence genes of Aggregatibacter actinomycetemcomitans can be expressed and confer fitness advantages in the closely related Aggregatibacter aphrophilus. Design Clinical isolates of A. aphrophilus were screened for natural competence with marked genomic DNA from A. actinomycetemcomitans and A. aphrophilus. The gene katA of A. actinomycetemcomitans D7S-1 and its flanking regions were constructed and inserted into a comparable locus in the genome of a naturally competent A. aphrophilus strain by a markerless protocol via natural transformation. Mutants of A. actinomycetemcomitans with or without katA were also constructed by a similar protocol. Discs soaked with either 0.03% hydrogen peroxide or broth culture of Streptococcus gordonii Challis were placed on the agar with cultures of A. actinomycetemcomitans or A. aphrophilus. The size of the growth inhibition zone associated with the disc was measured after 2-day culture. Results Five of the 13 A. aphrophilus strains exhibited a transformation frequency of 10−6 or higher. The intra- and inter-species transformation frequencies were comparable. The inhibition zones for katA-negative strains of A. actinomycetemcomitans or A. aphrophilus were 3- to 7-fold larger than those associated with katA-positive strains (p<0.05). Conclusions There was no apparent species barrier for the transfer and expression of A. actinomycetemcomitans katA in A. aphrophilus. The inserted A. actinomycetemcomitans-specific katA gene in A. aphrophilus strain NJ8700 conferred resistance to inhibition by hydrogen peroxide or S. gordonii. The potential to swap genes between these two closely related oral species may be an alternative approach for investigating the virulence determinants of A. actinomycetemcomitans.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.12.002
      Issue No: Vol. 86 (2017)
  • Extensive phenotyping of the orofacial and dental complex in Crouzon
    • Authors: Alexander Khominsky; Robin Yong; Sarbin Ranjitkar; Grant Townsend; Peter J. Anderson
      Pages: 123 - 130
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Alexander Khominsky, Robin Yong, Sarbin Ranjitkar, Grant Townsend, Peter J. Anderson
      Objectives Fibroblast growth factor receptor 2 (FGFR2) C342Y/+ mutation is a known cause of Crouzon syndrome that is characterised by craniosynostosis and midfacial hypoplasia. Our aim was to conduct extensive phenotyping of the maxillary, mandibular and dental morphology associated with this mutation. Materials and methods Morphometric data were obtained from 40 mice, representing two genotypes (Crouzon and wild-type) and two sexes (males and females) (n=10 in each group). Dental analysis further categorised the first molars into the two jaws (maxillary and mandibular) (n=20 in each group). Maxillary, mandibular and dental morphology was compared by analysing 23 linear landmark-based dimensions in three-dimensional micro-computed tomography reconstructions. Results Compared with wild-type, Crouzon (FGFR2C342Y/+ ) maxillae were significantly shorter in maximum height, anterior and posterior lengths and middle width, but larger in posterior width (p< 0.05 for height; p< 0.001 for other comparisons). In the Crouzon mandible, the ascending and descending heights, effective and mandibular lengths, and intercoronoid and intercondylar widths were significantly shorter, whereas intergonial width was larger (p< 0.01 for intercondylar width; p< 0.001 for other comparisons). Crouzon teeth were significantly smaller mesiodistally, but larger in crown height (p<0.001 for each comparison). All Crouzon mice presented with bifid mandibular condyles and a quarter presented with expansive bone lesions in the mandibular incisor alveolus. Conclusions Our findings of hypoplasia in all three planes in Crouzon maxillae and mandibles, together with the presence of bifid mandibular condyles and expansive bone lesions, may be relevant to maxillofacial surgery and orthodontics. Beyond skeletal effects, the FGFR2C342Y/+ mutation is now implicated in affecting tooth development. This study’s skeletal phenomics data also provides baseline data against which the effect of various treatments can now be assessed.

      PubDate: 2017-12-07T23:24:46Z
      DOI: 10.1016/j.archoralbio.2017.10.022
      Issue No: Vol. 86 (2017)
  • Dentin sialophosphoprotein expression in enamel is regulated by Copine-7,
           a preameloblast-derived factor
    • Authors: Su-Jin Park; Hye-Kyung Lee You-Mi Seo Chul Son Hyun Sook
      Abstract: Publication date: Available online 6 December 2017
      Source:Archives of Oral Biology
      Author(s): Su-Jin Park, Hye-Kyung Lee, You-Mi Seo, Chul Son, Hyun Sook Bae, Joo-Cheol Park
      Objective Dentin sialophosphoprotein (Dspp) is expressed in odontoblasts and transiently expressed in early ameloblasts. However, the origin of Dspp in ameloblasts remains unclear. Our previous studies demonstrated that copine-7 (CPNE7), a molecule that is secreted by the dental epithelium, is expressed in early ameloblasts and is then translocated to differentiating odontoblasts; its expression levels correlate with odontoblast differentiation under the control of Dspp expression. The objective of this study is to figure out the relationship between CPNE7 and Dspp during amelogenesis. Design The gene expression patterns of CPNE7 and dentin sialoprotein (DSP) were examined by immunohistochemistry, western blot analysis, and real-time polymerase chain reaction. The effects of CPNE7 on Dspp regulation were investigated using luciferase and chromatin immunoprecipitation assays in ameloblastic HAT-7 cells. Results The gene expression pattern of Cpne7 was similar to that of Dspp during ameloblast differentiation. Moreover, Gene expression omnibus profiles indicated that there is a close correlation between Cpne7 and Dspp expression in various normal human tissues. We also confirmed the effects of CPNE7 on the induction of Dspp in ameloblastic HAT-7 cells. Cpne7 overexpression promoted Dspp expression, whereas Dspp expression was down-regulated by Cpne7 inactivation. Conclusions These results suggest that the expression of Dspp in early amelogenesis is linked to CPNE7, a preameloblast-derived factor.

      PubDate: 2017-12-07T23:24:46Z
  • Regionally variant collagen alignment correlates with viscoelastic
           properties of the disc of the human temporomandibular joint
    • Authors: Shawn Gutman; Daniel Kim Solaiman Tarafder Sergio Velez Julia Jeong
      Abstract: Publication date: February 2018
      Source:Archives of Oral Biology, Volume 86
      Author(s): Shawn Gutman, Daniel Kim, Solaiman Tarafder, Sergio Velez, Julia Jeong, Chang H. Lee
      Objective To determine the regionally variant quality of collagen alignment in human TMJ discs and its statistical correlation with viscoelastic properties. Design For quantitative analysis of the quality of collagen alignment, horizontal sections of human TMJ discs with Pricrosirius Red staining were imaged under circularly polarized microscopy. Mean angle and angular deviation of collagen fibers in each region were analyzed using a well-established automated image-processing for angular gradient. Instantaneous and relaxation moduli of each disc region were measured under stress-relaxation test both in tensile and compression. Then Spearman correlation analysis was performed between the angular deviation and the moduli. To understand the effect of glycosaminoglycans on the correlation, TMJ disc samples were treated by chondroitinase ABC (C-ABC). Results Our imaging processing analysis showed the region-variant direction of collagen alignment, consistently with previous findings. Interestingly, the quality of collagen alignment, not only the directions, was significantly different in between the regions. The angular deviation of fiber alignment in the anterior and intermediate regions were significantly smaller than the posterior region. Medial and lateral regions showed significantly bigger angular deviation than all the other regions. The regionally variant angular deviation values showed statistically significant correlation with the tensile instantaneous modulus and the relaxation modulus, partially dependent on C-ABC treatment. Conclusion Our findings suggest the region-variant degree of collagen fiber alignment is likely attributed to the heterogeneous viscoelastic properties of TMJ disc that may have significant implications in development of regenerative therapy for TMJ disc.

      PubDate: 2017-11-15T16:18:10Z
  • Risk factors for tooth loss in middle and older age after up to 10 years:
           An observational cohort study
    • Authors: Alexander Jochen Hassel; Volkan Safaltin; Sabine Grill; Johannes Schröder; Hans-Werner Wahl; Anna-Luisa Klotz; Edriss Habibi; Peter Rammelsberg; Andreas Zenthöfer
      Abstract: Publication date: Available online 6 November 2017
      Source:Archives of Oral Biology
      Author(s): Alexander Jochen Hassel, Volkan Safaltin, Sabine Grill, Johannes Schröder, Hans-Werner Wahl, Anna-Luisa Klotz, Edriss Habibi, Peter Rammelsberg, Andreas Zenthöfer
      Objective The objective of this research was to identify risk factors for tooth loss in two older birth cohorts, quinquagenarians and septuagenarians, after up to 10 years of clinical observation. Design One hundred and twenty-three participants were recruited from the Interdisciplinary Study of Adult Development (ILSE) and examined at baseline and up to 10 years after. Thirty-nine and 84 participants belonged to the older (OC; born in 1930/32) cohort and younger (YC; born in 1950/52) cohort, respectively. Each participant underwent a dental examination comprising evaluation of the dental status (number of teeth, prosthetic restorations), Plaque Index (PI), Gingival Index (GI), DMF-S, periodontal probing depths (PD) and tooth mobility (TM). Incidence of tooth loss over the study period was calculated for both cohorts as well as for the grouped dental target variables. A logistic regression model for tooth loss (0=tooth present/1=tooth lost) was compiled with possible binary confounders. Results During the study period, 1.2 (1.9) and 2.6 (2.6) teeth were lost in YC and OC, respectively, reflecting correspondent loss rates of 5% and 14% (p<0.001). However, primarily TM >1 merged into substantial tooth loss (60%). The regression analysis confirmed the bivariate findings. Middle- and older-aged people, quinquagenarians and septuagenarians, show relevant tooth loss. Older age and worse oral health issues were identified as risk factors for tooth loss (p<0.007). Conclusions The predominant predictor of tooth loss seems to be tooth mobility. With the rising challenges due to aging in several societies, knowing the risks might help clinicians when weighing treatment strategies and should encourage refining preventive measures for older patients.

      PubDate: 2017-11-08T15:22:47Z
      DOI: 10.1016/j.archoralbio.2017.11.001
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