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Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 20)
Achievements in the Life Sciences     Open Access   (Followers: 4)
ACS Synthetic Biology     Full-text available via subscription   (Followers: 21)
Acta Biologica Colombiana     Open Access   (Followers: 6)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 4)
Acta Biologica Sibirica     Open Access  
Acta Biomaterialia     Hybrid Journal   (Followers: 25)
Acta Biotheoretica     Hybrid Journal   (Followers: 5)
Acta Chiropterologica     Full-text available via subscription   (Followers: 6)
acta ethologica     Hybrid Journal   (Followers: 4)
Acta Limnologica Brasiliensia     Open Access   (Followers: 3)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales : The Journal of Silesian Museum in Opava     Open Access  
Acta Parasitologica     Hybrid Journal   (Followers: 9)
Acta Scientiarum. Biological Sciences     Open Access   (Followers: 2)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
Actualidades Biológicas     Open Access   (Followers: 1)
Advanced Health Care Technologies     Open Access   (Followers: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Bioinformatics     Open Access   (Followers: 18)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4)
Advances in Biology     Open Access   (Followers: 8)
Advances in Biosensors and Bioelectronics     Open Access   (Followers: 6)
Advances in Cell Biology     Open Access   (Followers: 23)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Ecological Research     Full-text available via subscription   (Followers: 41)
Advances in Ecology     Open Access   (Followers: 16)
Advances in Environmental Sciences - International Journal of the Bioflux Society     Open Access   (Followers: 20)
Advances in Enzyme Research     Open Access   (Followers: 9)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Genome Biology     Full-text available via subscription   (Followers: 12)
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Advances in Human Biology     Open Access   (Followers: 1)
Advances in Life Science and Technology     Open Access   (Followers: 14)
Advances in Life Sciences     Open Access   (Followers: 4)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3)
Advances in Regenerative Biology     Open Access   (Followers: 1)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Virus Research     Full-text available via subscription   (Followers: 5)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 6)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 1)
Ageing Research Reviews     Hybrid Journal   (Followers: 8)
Aging Cell     Open Access   (Followers: 10)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat     Open Access  
AJP Cell Physiology     Full-text available via subscription   (Followers: 13)
AJP Endocrinology and Metabolism     Full-text available via subscription   (Followers: 22)
AJP Lung Cellular and Molecular Physiology     Full-text available via subscription   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Biology Teacher     Full-text available via subscription   (Followers: 12)
American Fern Journal     Full-text available via subscription   (Followers: 1)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 10)
American Journal of Bioethics     Hybrid Journal   (Followers: 10)
American Journal of Biostatistics     Open Access   (Followers: 9)
American Journal of Human Biology     Hybrid Journal   (Followers: 12)
American Journal of Medical and Biological Research     Open Access   (Followers: 5)
American Journal of Plant Sciences     Open Access   (Followers: 19)
American Journal of Primatology     Hybrid Journal   (Followers: 15)
American Malacological Bulletin     Full-text available via subscription   (Followers: 3)
American Naturalist     Full-text available via subscription   (Followers: 65)
Amphibia-Reptilia     Hybrid Journal   (Followers: 6)
Anaerobe     Hybrid Journal   (Followers: 4)
Analytical Methods     Full-text available via subscription   (Followers: 7)
Anatomical Science International     Hybrid Journal   (Followers: 2)
Animal Cells and Systems     Hybrid Journal   (Followers: 4)
Annales de Limnologie - International Journal of Limnology     Hybrid Journal   (Followers: 1)
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3)
Annales UMCS, Biologia     Open Access   (Followers: 1)
Annals of Applied Biology     Hybrid Journal   (Followers: 8)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Human Biology     Hybrid Journal   (Followers: 4)
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 17)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 25)
Annual Review of Cancer Biology     Full-text available via subscription  
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 38)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 14)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 19)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 10)
Anthropological Review     Open Access   (Followers: 25)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antibiotics     Open Access   (Followers: 8)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 8)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 8)
Applied Bionics and Biomechanics     Open Access   (Followers: 8)
Applied Vegetation Science     Full-text available via subscription   (Followers: 8)
Aquaculture Environment Interactions     Open Access   (Followers: 2)
Aquaculture International     Hybrid Journal   (Followers: 22)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 6)
Aquatic Biology     Open Access   (Followers: 4)
Aquatic Ecology     Hybrid Journal   (Followers: 30)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 13)
Aquatic Science and Technology     Open Access   (Followers: 3)
Aquatic Toxicology     Hybrid Journal   (Followers: 19)
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 3)
Archives of Biomedical Sciences     Open Access   (Followers: 7)
Archives of Microbiology     Hybrid Journal   (Followers: 8)
Archives of Natural History     Hybrid Journal   (Followers: 7)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 5)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arid Ecosystems     Hybrid Journal   (Followers: 3)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 2)
Arthropods     Open Access   (Followers: 1)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 2)
Artificial Photosynthesis     Open Access   (Followers: 1)
Asian Bioethics Review     Full-text available via subscription   (Followers: 1)
Asian Journal of Biodiversity     Open Access   (Followers: 5)
Asian Journal of Biological Sciences     Open Access   (Followers: 3)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Developmental Biology     Open Access   (Followers: 2)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 2)
Asian Journal of Nematology     Open Access   (Followers: 3)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 5)
Autophagy     Hybrid Journal   (Followers: 2)
Avian Biology Research     Full-text available via subscription   (Followers: 4)
Avian Conservation and Ecology     Open Access   (Followers: 10)
Bacteriology Journal     Open Access   (Followers: 2)
Bacteriophage     Full-text available via subscription   (Followers: 3)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access   (Followers: 1)
Berita Biologi     Open Access   (Followers: 1)
Between the Species     Open Access   (Followers: 1)
Bio Tribune Magazine     Hybrid Journal  
BIO Web of Conferences     Open Access  
BIO-Complexity     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bioanalytical Reviews     Hybrid Journal   (Followers: 2)
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 6)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 14)
Biochimie     Hybrid Journal   (Followers: 7)
BioControl     Hybrid Journal   (Followers: 5)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal   (Followers: 1)
Biodiversidad Colombia     Open Access  
Biodiversity : Research and Conservation     Open Access   (Followers: 26)
Biodiversity and Natural History     Open Access   (Followers: 5)
Biodiversity Data Journal     Open Access   (Followers: 3)
Biodiversity Informatics     Open Access  
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro     Open Access  
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 2)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 2)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
Bioethics     Hybrid Journal   (Followers: 14)
BioéthiqueOnline     Open Access  
Biofabrication     Hybrid Journal   (Followers: 3)
Biogeosciences (BG)     Open Access   (Followers: 10)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 1)
Bioinformatics     Hybrid Journal   (Followers: 297)
Bioinformatics and Biology Insights     Open Access   (Followers: 15)
Bioinspiration & Biomimetics     Hybrid Journal   (Followers: 6)
Biointerphases     Open Access   (Followers: 1)
Biojournal of Science and Technology     Open Access  
Biologia     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 4)
Biological Control     Hybrid Journal   (Followers: 5)
Biological Invasions     Hybrid Journal   (Followers: 16)
Biological Journal of the Linnean Society     Hybrid Journal   (Followers: 15)
Biological Letters     Open Access   (Followers: 4)
Biological Procedures Online     Open Access  
Biological Psychiatry     Hybrid Journal   (Followers: 42)
Biological Psychology     Hybrid Journal   (Followers: 6)
Biological Research     Open Access  
Biological Rhythm Research     Hybrid Journal   (Followers: 2)
Biological Theory     Hybrid Journal   (Followers: 1)
Biological Trace Element Research     Hybrid Journal  
Biologicals     Full-text available via subscription   (Followers: 9)
Biologics: Targets & Therapy     Open Access   (Followers: 1)
Biologie Aujourd'hui     Full-text available via subscription  
Biologie in Unserer Zeit (Biuz)     Hybrid Journal   (Followers: 42)
Biologija     Open Access  
Biology     Open Access   (Followers: 5)
Biology and Philosophy     Hybrid Journal   (Followers: 16)
Biology Bulletin     Hybrid Journal   (Followers: 1)
Biology Bulletin Reviews     Hybrid Journal  
Biology Direct     Open Access   (Followers: 7)
Biology Letters     Full-text available via subscription   (Followers: 35)

        1 2 3 4 5 6 7 8 | Last

Journal Cover Archives of Oral Biology
  [SJR: 0.713]   [H-I: 64]   [2 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0003-9969
   Published by Elsevier Homepage  [3042 journals]
  • Does pain in the masseter and anterior temporal muscles influence maximal
           bite force'
    • Authors: Marcelo Coelho Goiato; Paulo Renato Junqueira Zuim; Amália Moreno; Daniela Micheline dos Santos; Emily Vivianne Freitas da Silva; Fernanda Pereira de Caxias; Karina Helga Leal Turcio
      Pages: 1 - 6
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Marcelo Coelho Goiato, Paulo Renato Junqueira Zuim, Amália Moreno, Daniela Micheline dos Santos, Emily Vivianne Freitas da Silva, Fernanda Pereira de Caxias, Karina Helga Leal Turcio
      Objective The aim of this study was to evaluate changes in pain and muscle force, and the relationship between them, in patients with muscle pain and bruxism, prior to and after treatment. Methods Thirty women with bruxism and myofascial pain (Ia) were included in this study. Sleep bruxism diagnosis was made based on clinical diagnostic criteria, and awake bruxism diagnosis was made by patient questionnaires and the presence of tooth wear. The diagnosis of myofascial pain was established according to the Research Diagnostic Criteria for Temporomandibular Disorders (RDC-TMD). Dentulous or partially edentulous patients (rehabilitated with conventional fixed prostheses) were included in the study according to the inclusion and exclusion criteria. The pain treatment protocol included occlusal splints, patient education, and physiotherapy for 30days. Bite force was measured using a dynamometer at the central incisor and the first molar regions on both sides. The exams were performed at baseline, after 7days, and 30days after treatment. The Wilcoxon test was used to compare patient pain level response among the periods analyzed in the study. Bite force data were submitted to two-way repeated-measures ANOVA, followed by the Tukey HSD test (p <0.05). A simple regression analysis was performed to verify the relation between pain level and bite force. Results Results revealed that there was a statistical difference in pain level over time for both muscles and sides (p <0.01). In the molar region, the bite force exhibited significantly higher values after 30days of treatment, when compared with the baseline (p <0.001). There was a correlation between pain level and bite force only for the temporal muscle in all periods analyzed (p<0.05). There was no strong correlation in the response level points to support the association of pain and bite force. Conclusions Pain level decreased and bite force increased in the molar region after treatment. No strong correlation or dispersion in the relationship between pain levels and bite force was seen in women with myofascial pain and bruxism.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.029
      Issue No: Vol. 83 (2017)
  • Pathogenicity and genetic profile of oral Porphyromonas species from
           canine periodontitis
    • Authors: Amanda do Nascimento Silva; Erica Dorigatti de Avila; Viviane Nakano; Mario J. Avila-Campos
      Pages: 20 - 24
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Amanda do Nascimento Silva, Erica Dorigatti de Avila, Viviane Nakano, Mario J. Avila-Campos
      Objective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.07.001
      Issue No: Vol. 83 (2017)
  • Sleep bruxism and related risk factors in adults: A systematic literature
    • Authors: Tommaso Castroflorio; Andrea Bargellini; Gabriele Rossini; Giovanni Cugliari; Andrea Deregibus
      Pages: 25 - 32
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Tommaso Castroflorio, Andrea Bargellini, Gabriele Rossini, Giovanni Cugliari, Andrea Deregibus
      Objective The aim of this article was to systematically review the literature to assess the relationship between risk factors and sleep bruxism (SB) in adults (age ≥18 years). Design A systematic search of the following databases was carried out: PubMed, Embase, Scopus, Cochrane Oral Health Group’s Trial Register and Cochrane Register of Controlled Trials, Web of Science, LILACs and SciELO. Nine out of the 4583 initially identified articles were selected. This review was conducted according to the guidelines from the Cochrane Handbook for Systematic Reviews of Interventions, with reporting in agreement to the Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines. Results Among the nine analyzed articles, associations between SB and gastro-esophageal reflux disease (GERD) (OR=6.6, CI=1.4–30.9) was found in one randomized clinical trial (RCT). Four cross-sectional studies suggested history of SB during childhood (OR=8.1 CI=5.4–12–2), age (OR=3.1, CI=2.3–4.1) and chronic migraine (OR=3.8, C.I=1.8–7.8) as determinant factors for the development of SB. In one case-control study, patients with genetic polymorphisms were more likely to present SB (OR=4.3, CI=1.6–11.3). Smoking (OR=2.8, CI=2.2–3.5) and alcohol intake (OR=1.9, CI=1.2–2.8) showed moderate association in two case-control studies. Conclusions History of SB during childhood, gastro-esophageal reflux disease and genetic polymorphisms seem to be important risk factors associated to SB in adults. Dry mouth on awakening seems to be a protective factor. Association does not infer with causality. Even if the evidence emerged from the considered studies was clinically relevant, further studies are requested to better understand the biological mechanisms behind the described associations.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.07.002
      Issue No: Vol. 83 (2017)
  • Effects of melatonin on the proliferation and differentiation of human
           dental pulp cells
    • Authors: Qin Liu; Wenguo Fan; Yifan He; Fuping Zhang; Xiaoyan Guan; Qianyi Deng; Xianjun Lu; Hongwen He; Fang Huang
      Pages: 33 - 39
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Qin Liu, Wenguo Fan, Yifan He, Fuping Zhang, Xiaoyan Guan, Qianyi Deng, Xianjun Lu, Hongwen He, Fang Huang
      Objective Effects of melatonin on the proliferation and differentiation of human dental pulp cells (hDPCs) remain unclear. The purpose of this study was to investigate the effect of melatonin on the proliferation and differentiation of the hDPCs. Design Primary hDPCs were obtained from the third molar of volunteer aged from 18 to 25. CCK8 assay evaluated the effect of melatonin upon cell proliferation at day 1, 2, 3, 4, 5. After 7days’ osteogenic induction with melatonin or vehicle, alkaline phosphatase (ALP) activity was measured with a commercial kit. Then levels of dentin sialophosphoprotein (DSPP) were determined by immunocytochemical staining and western blot analysis, followed by quantitative real-time reverse transcription-Polymerase chain reaction (qRT-PCR) to analyse mRNA levels of ALP and DSPP. Finally hDPCs exposed to osteogenic medium containing melatonin or vehicle for 14days were stained with alizarin red to detect mineralization nodules formation. Results Melatonin significantly inhibited the proliferative ability of the hDPCs in a concentration- and time-dependent manner. The hDPCs cultured in osteogenic induction medium with melatonin presented an increase of ALP activity, expression of DSPP, mRNA levels of ALP and DSPP, and mineralization nodules formation. Conclusions These findings indicate that melatonin at physiological concentrations can inhibit proliferation and promote the differentiation of hDPCs, which might give some new insights into the mechanism of regulating DPCs to achieve dentine regeneration.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.034
      Issue No: Vol. 83 (2017)
  • Influence of the RPL34 gene on the growth and metastasis of oral squamous
           cell carcinoma cells
    • Authors: Jing Dai; Wei Wei
      Pages: 40 - 46
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Jing Dai, Wei Wei
      Background Oral squamous cell carcinoma (OSCC) accounts for almost 3% of all malignant tumors all over the world. This study aims to investigate the correlation of RPL34 with the cell growth and metastasis of oral squamous cell carcinoma (OSCC) as well as its clinical prognosis. Method Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were conducted to determine the RPL34 expression in 85 OSCC tissues and 30 normal oral mucosa tissues. Besides, OSCC cell lines SCC-4 were divided into blank group, negative control (NC) group and RPL34-shRNA group. The qRT-PCR and western blot were performed to measure RPL34 expression, CCK-8 and flow cytometry to observe cell growth and apoptosis, and wound healing and transwell to detect cell migration and invasion. Results The RPL34 gene expression was up-regulated in OSCC tissues and cells. The RPL34 expression was significantly correlated with differentiation degree, TNM stage and lymph node metastasis. Patients with positive RPL34 expression had a poorer prognosis. After inhibition of RPL34 expression, the proliferation of SCC-4 cells was slowed down at 24h, 48h, 72h and 96h respectively, and both the migration distance and the number of invasive cells were reduced, while there was an increase in the ratio of cells at G0/G1 stage and cell apoptosis. Conclusion The RPL34 gene was highly expressed in OSCC, while silencing RPL34 could block cell proliferation and metastasis, but promote cell apoptosis, suggesting the RPL34 gene to be a new promising clinical target for OSCC therapy.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.035
      Issue No: Vol. 83 (2017)
  • Gingival crevicular fluid and plasma oxidative stress markers and TGM-2
           levels in chronic periodontitis
    • Authors: Sema Becerik; Veli Özgen Öztürk; Peter Celec; Natalia Kamodyova; Gül Atilla; Gülnur Emingil
      Pages: 47 - 54
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Sema Becerik, Veli Özgen Öztürk, Peter Celec, Natalia Kamodyova, Gül Atilla, Gülnur Emingil
      Objective This study was aimed to evaluate the gingival crevicular fluid (GCF) and plasma transglutaminase-2 (TGM-2), total antioxidant capacity (TAC), total oxidant status (TOS), ferric reducing antioxidant power (FRAP) and thiobarbituric acid reactive substances (TBARS) in patients with chronic periodontal disease. Materials and methods Twenty patients with chronic periodontitis (CP), 20 patients with gingivitis and 20 healthy subjects were enrolled in the study. Clinical periodontal parameters including probing depth, clinical attachment level, plaque index and papillary bleeding index were recorded. GCF and plasma levels of TGM-2, TAC, TOS, TBARS and FRAP were analyzed. Results GCF TGM-2 was significantly lower in CP group than in gingivitis patients (P=0.006). GCF FRAP in CP and gingivitis groups was significantly lower than in healthy subjects (P<0.001). Plasma FRAP level was lower in gingivitis group when compared to healthy subjects (P=0.003). There was no significant difference in GCF and plasma TAC, TOS, TBARS and plasma TGM-2 levels among the study groups (P>0.05). GCF TGM-2 level was positively correlated with GCF TAC and negatively correlated with CAL. Conclusions Decreased FRAP in GCF and plasma indicating lower antioxidant status of CP patients might suggest the role of oxidative stress in periodontitis. GCF TGM-2 data might suggest that TGM2 is associated with stabilization of the extracellular matrix and wound healing in periodontium rather than gingival inflammation.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.06.032
      Issue No: Vol. 83 (2017)
  • Immunohistochemical investigations on the expression of programmed cell
           death ligand 1, human leukocyte antigens G and E, and granzyme B in
           intraoral mucoepidermoid carcinoma
    • Authors: Carla Mosconi; Diego Antônio Costa Arantes; Andréia Souza Gonçalves; Rita de Cássia Gonçalves Alencar; José Carlos Oliveira; Tarcília Aparecida Silva; Elismauro Francisco Mendonça; Aline Carvalho Batista
      Pages: 55 - 62
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Carla Mosconi, Diego Antônio Costa Arantes, Andréia Souza Gonçalves, Rita de Cássia Gonçalves Alencar, José Carlos Oliveira, Tarcília Aparecida Silva, Elismauro Francisco Mendonça, Aline Carvalho Batista
      Objective To identify the expression of nonclassical human leukocyte antigen G and E (HLA-G and -E), programmed cell death ligand-1 (PD-L1) and granzyme B (GB) in intraoral mucoepidermoid carcinomas (MECs), and to assess whether such expressions are related to metastasis, survival, staging, tumor grade and number of GB-positive cells. Design For this cross-sectional study, samples of MEC (n=30) were selected and classified as low-grade (LG), intermediate-grade (IG) or high-grade (HG), according to the WHO grading system. HLA-G, -E and PD-L1 were identified by immunohistochemistry and quantified as the proportion of positive neoplastic cells. The density of GB+ cells was also evaluated. The Kruskal-Wallis test was used with a 5% significance level. Results Expressions of HLA-G, -E and PD-L1 were identified in the majority of epidermoid, intermediate and clear cells, but not in the mucous cells of the MECs. The quantitative analysis of the total percentage of positive neoplastic cells showed overexpression of this set of proteins in all MEC samples. The expression of these proteins and histological grading were positively correlated [HLA-G (LG=79% positive cells, IG=96%, HG=99%; p =0.0004), HLA-E (LG=70%, IG=96%, HG=99%; p< 0.0001) and PD-L1 (LG=34%, IG=79%, HG=80%; p =0.01)]. No relationship was observed between the immunosuppressive proteins and other clinicopathological parameters. Low GB density was found in all MEC samples. Conclusions The augmented expression of HLA-G, -E and PD-L1 in the intraoral MEC might suggest a role of these molecules in the scape of neoplastic cells from immunosurveillance.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.07.004
      Issue No: Vol. 83 (2017)
  • Expression of MyHC isoforms mRNA transcripts in different regions of the
           masseter and medial pterygoid muscles in chimpanzees
    • Authors: Neus Ciurana; Rosa Artells; Carmen Muñoz; Júlia Arias-Martorell; Gaëlle Bello-Hellegouarch; Alejandro Pérez-Pérez; Juan Francisco Pastor; Josep Maria Potau
      Pages: 63 - 67
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Neus Ciurana, Rosa Artells, Carmen Muñoz, Júlia Arias-Martorell, Gaëlle Bello-Hellegouarch, Alejandro Pérez-Pérez, Juan Francisco Pastor, Josep Maria Potau
      Objective The aim of this study is to examine the expression pattern of the different myosin heavy chain (MyHC) isoforms in the masseter and medial pterygoid muscles by real time quantitative polymerase chain reaction (RT-qPCR) to obtain information at molecular level which can be related to the functional characteristics of these two muscles. Design The masseter, deep and superficial portion, and medial pterygoid muscles of five adult Pan troglodytes were dissected in order to obtain samples of the anterior and posterior regions of each portion of the masseter and of the medial pterygoid. The expression of MyHC isoforms mRNA transcripts was analyzed by RT-qPCR. Results No significant differences in expression of MyHC isoforms between the masseter and the medial pterygoid were found. In contrast, when comparing the superficial and the deep portion of the masseter, we found that the MyHC-IIM isoform was expressed at a significantly higher level in the superficial portion. Conclusions The superficial portion of the masseter and the medial pterygoid muscle have the same expression pattern regarding the different MyHC isoforms. On the other hand, the deep portion of the masseter, which is activated mainly during lateral and repositioning movements of the mandible, has a lower MyHC-IIM isoform expression than the superficial portion. Our findings provide new data on functional aspects of the masseter and medial pterygoid that can complement results obtained by other techniques.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.07.003
      Issue No: Vol. 83 (2017)
  • In silico search of inhibitors of Streptococcus mutans for the control of
           dental plaque
    • Authors: Rodrigo Ochoa; María Cecilia Martínez-Pabón; María Adelaida Arismendi-Echeverri; Willer Leandro Rendón-Osorio; Carlos Enrique Muskus-López
      Pages: 68 - 75
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Rodrigo Ochoa, María Cecilia Martínez-Pabón, María Adelaida Arismendi-Echeverri, Willer Leandro Rendón-Osorio, Carlos Enrique Muskus-López
      Biofilm is an extremely complex microbial community arranged in a matrix of polysaccharides and attached to a substrate. Its development is crucial in the pathophysiology of oral infections like dental caries, as well as in periodontal, pulp, and periapical diseases. Streptococcus mutans is one of the most effective microorganisms in lactic acid production of the dental biofilm. Identifying essential Streptococcus mutans proteins using bioinformatics methods helps to search for alternative therapies. To this end, the bacterial genomes of several Streptococcus mutans strains and representative strains of other cariogenic and non-cariogenic bacteria were analysed by identifying pathogenicity islands and alignments with other bacteria, and by detecting the exclusive genes of cariogenic species in comparison to the non-pathogenic ones. This study used tools for orthology prediction such as BLAST and OrthoMCL, as well as the server IslandViewer for the detection of pathogenicity islands. In addition, the potential interactome of Streptococcus mutans was rebuilt by comparing it to interologues of other species phylogenetically close to or associated with cariogenicity. This protocol yielded a final list of 20 proteins related to potentially virulent factors that can be used as therapeutic targets in future analyses. The EIIA and EIIC enzymatic subunits of the phosphotransferase system (PTS) were prioritized, as well as the pyruvate kinase enzyme, which are directly involved in the metabolism of carbohydrates and in obtaining the necessary energy for the microorganism’s survival. These results will guide a subsequent experimental trial to develop new, safe, and effective molecules in the treatment of dental caries.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.06.027
      Issue No: Vol. 83 (2017)
  • Intermittent administration of parathyroid hormone ameliorated alveolar
           bone loss in experimental periodontitis in streptozotocin-induced diabetic
    • Authors: Helin Chen; Taozi Fu; Yuanyuan Ma; Xiangnan Wu; Xianxian Li; Xinyi Li; Jiefei Shen; Hang Wang
      Pages: 76 - 84
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Helin Chen, Taozi Fu, Yuanyuan Ma, Xiangnan Wu, Xianxian Li, Xinyi Li, Jiefei Shen, Hang Wang
      Objective Intermittent administration of parathyroid hormone (PTH) has been demonstrated to have anabolic effects on bone metabolism and is approved for use in the treatment of osteoporosis. This study evaluates the role of intermittent PTH administration on alveolar bone loss in streptozotocin (STZ)-induced diabetic rats. Design Fifty male Sprague Dawley rats were randomly divided into the following five groups: (1) a control group (saline placebo without ligature and STZ injection), (2) a PTH group (PTH administration without ligature and STZ injection), (3) an L group (saline placebo with ligature), (4) an L+STZ group (saline placebo with ligature and STZ injection), and (5) an L+STZ+PTH group (PTH administration with ligature and STZ injection). PTH was administered at 75μg/kg per dose four times a week for 28days. Subsequently, all rats were sacrificed, and their mandibles were extracted for micro-computed tomography (micro-CT) scanning, as well as histological and immunochemical evaluation. Results Micro-CT scanning demonstrated the anabolic effect of PTH on alveolar bone metabolism in STZ-induced diabetic rats (P<0.05), and histomorphometry indicated that PTH inhibited inflammation of the periodontium and increased the level of osteoblastic activity (P<0.05). Immunochemical evaluation showed that rats subjected to both ligature placement and STZ injection had the highest receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio and that PTH administration decreased this ratio. Conclusion Intermittent systemic PTH administration effectively reduced alveolar bone loss and ameliorated the manifestation of experimental periodontitis in STZ-induced diabetic rats.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.06.033
      Issue No: Vol. 83 (2017)
  • Expression of planar cell polarity genes during mouse tooth development
    • Authors: Nobuko Obara; Yuko Suzuki; Kazuharu Irie; Shunichi Shibata
      Pages: 85 - 91
      Abstract: Publication date: November 2017
      Source:Archives of Oral Biology, Volume 83
      Author(s): Nobuko Obara, Yuko Suzuki, Kazuharu Irie, Shunichi Shibata
      Objective Planar cell polarity (PCP) refers to the cell polarity across the tissue plane and controls various cell behaviors and structures. Although the expression of several PCP signaling components has been detected in tooth germs, knowledge of the gene expression patterns of these PCP components during tooth development remains incomplete. The aim of this study is to characterize the temporal and spatial changes in PCP gene expression during tooth development. Design Expression of Celsr1 and 2, Fzd3 and 6, Vangl1 and 2, and Dvl1-3 genes was analyzed in mouse molar germs from the bud to the bell stage using in situ hybridization. Results At the bud stage, all target genes were expressed in all areas of the tooth bud. In the enamel organ at the cap stage, expression of Fzd3 was suppressed in the enamel knot, whereas Fzd6 was strongly expressed there. Expression of Vangl2 was strongly expressed in the inner dental epithelium from the cap stage onwards. In the inner dental epithelium, strong expression of Fzd3, Dvl2 and Vangl2 was noted at the early bell stage, and of Celsr1, Fzd3, Fzd6, Vangl2 and Dvl2 at the bell stage. Furthermore, differentiated odontoblasts strongly expressed Celsr1, Vangl2, and Dvl2. Conclusion The gene expression patterns delineated in this study improve our understanding of the role(s) of PCP components during tooth development.

      PubDate: 2017-07-20T03:35:10Z
      DOI: 10.1016/j.archoralbio.2017.07.008
      Issue No: Vol. 83 (2017)
  • The effect of subinhibitory concentrations of gentian violet on the germ
           tube formation by Candida albicans and its adherence to oral epithelial
    • Authors: T. Mafojane; S.L. Shangase; M. Patel
      Pages: 1 - 5
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): T. Mafojane, S.L. Shangase, M. Patel
      Objective This study investigated the effect of subinhibitory concentrations of gentian violet on the germ tube formation by Candida albicans and its adherence ability to oral epithelial cells. Methods Thirty strains of C. albicans isolated from denture wearers, normal healthy individuals and HIV positive patients were used in the study. The antifungal property (Minimum Fungicidal Concentration) of gentian violet was determined at various time intervals using a microdilution technique. The effect of subinhibitory concentrations of gentian violet on the adherence ability (0.000244%) and on germ tube formation ((0.000244%, 0.000122%, 0.000061% and 0.000031%) was determined. In both experiments, water was used as a control. The test results were compared using the Kruskal-Wallis test. Results At 60min a high concentration (0.0078%) of gentian violet was required to completely kill C. albicans. Subinhibitory concentrations of gentian violet significantly reduced the adherence ability of C. albicans by 57% (p<0.01) and equally inhibited germ tube formation (p<0.01) compared with the controls. The inhibition was concentration dependent, with up to 98% reduction at a concentration of 0.000244%. Germ tube reduction was significantly higher in the isolates from the HIV positive patients than in the isolates from denture wearers. Conclusion At high concentrations, gentian violet killed C. albicans, whereas at subinhibitory concentrations it reduced its virulence by preventing the adherence ability and germ tube formation. This suggests that the beneficial effects of gentian violet would last beyond the fungicidal concentrations in the treatment of candidiasis.
      Graphical abstract image

      PubDate: 2017-06-02T05:20:54Z
      DOI: 10.1016/j.archoralbio.2017.05.016
      Issue No: Vol. 82 (2017)
  • Denaturing gradient gel electrophoresis profiles of bacteria from the
           saliva of twenty four different individuals form clusters that showed no
           relationship to the yeasts present
    • Authors: Manjula M Weerasekera; Chris H Sissons; Lisa Wong; Sally A Anderson; Ann R Holmes; Richard D Cannon
      Pages: 6 - 10
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Manjula M Weerasekera, Chris H Sissons, Lisa Wong, Sally A Anderson, Ann R Holmes, Richard D Cannon
      Objectives The aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the DGGE fingerprints and the amounts and diversity of yeast present. Methods Bacterial and yeast populations in saliva samples from 24 adults were analysed using denaturing gradient gel electrophoresis (DGGE) of the bacteria present and by yeast culture. Results Eubacterial DGGE banding patterns showed considerable variation between individuals. Seventy one different amplicon bands were detected, the band number per saliva sample ranged from 21 to 39 (mean±SD=29.3±4.9). Cluster and principal component analysis of the bacterial DGGE patterns yielded three major clusters containing 20 of the samples. Seventeen of the 24 (71%) saliva samples were yeast positive with concentrations up to 103 cfu/mL. Candida albicans was the predominant species in saliva samples although six other yeast species, including Candida dubliniensis, Candida tropicalis, Candida krusei, Candida guilliermondii, Candida rugosa and Saccharomyces cerevisiae, were identified. The presence, concentration, and species of yeast in samples showed no clear relationship to the bacterial clusters. Conclusion Despite indications of in vitro bacteria-yeast interactions, there was a lack of association between the presence, identity and diversity of yeasts and the bacterial DGGE fingerprint clusters in saliva. This suggests significant ecological individual-specificity of these associations in highly complex in vivo oral biofilm systems under normal oral conditions.
      Graphical abstract image

      PubDate: 2017-06-02T05:20:54Z
      DOI: 10.1016/j.archoralbio.2017.05.014
      Issue No: Vol. 82 (2017)
  • Agomelatine, a MT1/MT2 melatonergic receptor agonist with serotonin 5-HT2C
           receptor antagonistic properties, suppresses Prevotella intermedia
           lipopolysaccharide-induced production of proinflammatory mediators in
           murine macrophages
    • Authors: Jin-Yi Hyeon; Eun-Young Choi; So-Hui Choe; Hae Ryoun Park; Jeom-Il Choi; In Soon Choi; Sung-Jo Kim
      Pages: 11 - 18
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Jin-Yi Hyeon, Eun-Young Choi, So-Hui Choe, Hae Ryoun Park, Jeom-Il Choi, In Soon Choi, Sung-Jo Kim
      Objective This study was performed in an attempt to examine the influence of agomelatine in mitigating the generation of proinflammatory mediators in RAW264.7 murine macrophages exposed to lipopolysaccharide (LPS) obtained from Prevotella intermedia, a gram-negative anaerobic bacterium that is related with various types of periodontal diseases, and the molecular mechanisms behind its effects. Design LPS from P. intermedia strain ATCC 25611 was prepared employing the conventional phenol-water procedure. Conditioned culture media were analyzed for the levels of nitric oxide (NO), interleukin-1β (IL-1β) and IL-6. Real-time PCR analysis was carried out to determine the mRNA levels of inducible NO synthase (iNOS), IL-1β, IL-6 and SOCS1. Protein expression levels were evaluated by immunoblot test. NF-κB-dependent SEAP reporter assay was performed using a reporter cell line. DNA-binding activities of NF-κB subunits were analyzed utilizing the ELISA-based kits. Results Agomelatine was found to down-regulate significantly the generation of iNOS-derived NO, IL-1β and IL-6 as well as the expression of their mRNAs in cells activated with P. intermedia LPS. Agomelatine decreased NF-κB-dependent SEAP release caused by P. intermedia LPS. Agomelatine did not inhibit NF-κB transcription induced by LPS at the level of IκB-α degradation. Instead, LPS-induced nuclear translocation and DNA binding of NF-κB p50 subunit was blocked by agomelatine. P. intermedia LPS-elicited activation of STAT1 and STAT3 was reduced notably by co-treatment with agomelatine. Agomelatine showed a tendency to enhance mRNA level of SOCS1 in LPS-activated cells as well. Conclusions Agomelatine merits further evaluation to reveal its usefulness on the host modulation of periodontal disease.

      PubDate: 2017-06-02T05:20:54Z
      DOI: 10.1016/j.archoralbio.2017.05.015
      Issue No: Vol. 82 (2017)
  • Differential expression of transcription factors NF-κB and STAT3 in
           periodontal ligament fibroblasts and gingiva of healthy and diseased
    • Authors: R. Ambili; Prasanthila Janam; P.S. Saneesh Babu; Manu Prasad; D. Vinod; P.R. Anil Kumar; T.V. Kumary; S. Asha Nair
      Pages: 19 - 26
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): R. Ambili, Prasanthila Janam, P.S. Saneesh Babu, Manu Prasad, D. Vinod, P.R. Anil Kumar, T.V. Kumary, S. Asha Nair
      Objective Pathogens and host mediators can activate transcription factors in periodontal cells to bring about gene level alterations, thereby accentuating the periodontal disease process. Nuclear factor-kappa B (NF-κB) and signal transducers and activators of transcription 3 (STAT3) are two pivotal transcription factors implicated in chronic inflammatory diseases. But their importance in periodontal pathogenesis has not been investigated in detail. The aim of the present study was to evaluate the expression of activated transcription factors and their target genes in healthy and diseased periodontium. Design Primary culture of periodontal ligament fibroblasts (PDLF) were established from healthy and diseased periodontium using explant culture methods. NF-κB and STAT3 activation in these cells by Porphyromonas gingivalis LPS (lipopolysaccharide) was demonstrated using confocal microscopy and mRNA expression of target genes were evaluated by quantitative real time PCR. NF-κB and STAT3 expression in diseased and healthy gingival tissues were analyzed using immunohistochemistry. Results A basal upregulation of transcription factors and their target genes were noted in diseased PDLF compared to healthy ones. LPS challenge induced differential expression of NF-κB and STAT3 and their target genes in diseased PDLF compared to healthy ones. Immunohistochemical analysis revealed significant activation of transcription factors in diseased gingival tissues. Conclusion The findings of the present study reveal the role of transcription factors NF-κB and STAT3 in periodontal pathogenesis and disease susceptibility of fibroblast subpopulations in periodontal disease could be mediated through activation of NF-κB and STAT3. Since genetic factors are nonmodifyable, transcription factors are promising targets for future host modulation therapy.

      PubDate: 2017-06-02T05:20:54Z
      DOI: 10.1016/j.archoralbio.2017.05.010
      Issue No: Vol. 82 (2017)
  • Decreased levels of matrix metalloproteinase-2 in root-canal exudates
           during root canal treatment
    • Authors: Kassara Pattamapun; Sira Handagoon; Thanapat Sastraruji; James L. Gutmann; Prasit Pavasant; Suttichai Krisanaprakornkit
      Pages: 27 - 32
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Kassara Pattamapun, Sira Handagoon, Thanapat Sastraruji, James L. Gutmann, Prasit Pavasant, Suttichai Krisanaprakornkit
      Objective To determine the matrix metalloproteinase-2 (MMP-2) levels in root-canal exudates from teeth undergoing root-canal treatment. Material and methods The root-canal exudates from six teeth with normal pulp and periradicular tissues that required intentional root canal treatment for prosthodontic reasons and from twelve teeth with pulp necrosis and asymptomatic apical periodontitis (AAP) were sampled with paper points for bacterial culture and aspirated for the detection of proMMP-2 and active MMP-2 by gelatin zymography and the quantification of MMP-2 levels by ELISA. Results By gelatin zymography, both proMMP-2 and active MMP-2 were detected in the first collection of root-canal exudates from teeth with pulp necrosis and AAP, but not from teeth with normal pulp, and their levels gradually decreased and disappeared at the last collection. Consistently, ELISA demonstrated a significant decrease in MMP-2 levels in the root-canal exudates of teeth with pulp necrosis and AAP following root canal procedures (p <0.05). Furthermore, the MMP-2 levels were significantly lower in the negative bacterial culture than those in the positive bacterial culture (p <0.001). Conclusions The levels of MMP-2 in root-canal exudates from teeth with pulp necrosis and AAP were gradually reduced during root canal procedures. Future studies are required to determine if MMP-2 levels may be used as a biomolecule for the healing of apical lesions, similar to the clinical application of MMP-8 as a biomarker.

      PubDate: 2017-06-07T05:23:10Z
      DOI: 10.1016/j.archoralbio.2017.05.019
      Issue No: Vol. 82 (2017)
  • Differential expression between “DSP-only” and DSP-PP523
           transcripts in rat molar teeth
    • Authors: Ya-Qin Zhu; Ryan M. Song; Helena H. Ritchie
      Pages: 33 - 37
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Ya-Qin Zhu, Ryan M. Song, Helena H. Ritchie
      Objective To compare the expression patterns of two multiple transcripts derived from DSP-PP gene during tooth development. One is DSP-only transcript (i.e. does not encode PP) and the other is DSP-PP523 transcript, a main DSP-PP transcript. Design Unique antisense and sense riboprobes were generated from DSP-only and DSPPP523 cDNAs for in situ studies to examine DSP-only and DSP-PP523 transcript expression in developing molars. Paraffin-embedded sections (5–7μ m) from embryonic 20day, postnatal 2, 3 and 6days were deparaffined and hydrated. Tissues were prehybridized, then hybridized with DSP-only and DSP-PP523 anti-sense (AS) or sense (S) Digoxigenin labeled-riboprobes overnight, and washed. Anti-Digoxigenin antibodies conjugated to alkaline phosphatase were used to detect the presence of bound riboprobes by color reaction with NBT/BCIP. Stro-1 antibody was used for immunohistochemical analysis of Stro-1 protein expression in rat molars. Results We found that unlike the DSP-PP523 transcript, the DSP-only transcript does not express in the entire polarized mature odontoblasts but is expressed in the areas subjacent to the mature odontoblast layer. In addition, DSP-only transcript is expressed in the dental pulp. Interestingly, Stro-1 protein, a stem cell marker, was also identified in the areas subjacentto odontoblasts and in dental pulp. Conclusion Differential expression of DSP-only and DSP-PP523 transcripts suggest that these two kinds of transcripts may play different roles during dentinogenesis. DSP-PP523 transcript is expressed in mature odontoblasts, which actively participates in dentin formation. DSP-only transcript might have a different function.

      PubDate: 2017-06-07T05:23:10Z
      DOI: 10.1016/j.archoralbio.2017.04.031
      Issue No: Vol. 82 (2017)
  • Inflammatory cytokines in normal and irreversibly inflamed pulps: A
           systematic review
    • Authors: Vivian Hirsch; Michael Wolgin; Aleksandr V. Mitronin; Andrej M. Kielbassa
      Pages: 38 - 46
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Vivian Hirsch, Michael Wolgin, Aleksandr V. Mitronin, Andrej M. Kielbassa
      Objective To review the available literature in regard to the inflammatory process and pulpitis. Setting forth to evaluate if differences in the levels of various cytokines (TNF-α, IL-1β, IL-2, IL-6 and IL-8) can be observed in clinically diagnosed normal and irreversibly inflamed pulps that could serve as possible markers and/or diagnostic tools to predict and differentiate between certain states of inflammation. Methods used to measure and assess levels of cytokines have been limited to two protein quantification methods ELISA and/or Multiplex Array. Design The databases PubMed, EMBASE/Ovid, The Cochrane Central Register of Controlled Trials, Cochrane Reviews and Scopus were consulted for the electronic literature search. Screening of titles and abstracts followed the PRISMA guidelines while data extraction and the assessment of the full texts were carried out in accordance to the GRADES assessment. Results The review showed that significant increases in levels of IL-1β, IL-2, IL-6, IL-8 and TNF-α in irreversible pulpitis samples exist, in comparison to normal pulp samples which serve as a good basis for potential markers. Due to larger discrepancies in available literature, IL-2 seems rather unsuitable at the moment, while IL-6 and TNF alpha seem to be more promising. Conclusion It may be concluded that even by combining two protein quantification methods inconsistencies between studies exist. At the moment it is difficult to select just one specific cytokine suitable for testing, rather it supports the rationale that further high-quality clinical studies are needed.

      PubDate: 2017-06-12T05:29:59Z
      DOI: 10.1016/j.archoralbio.2017.05.008
      Issue No: Vol. 82 (2017)
  • Intermittent compressive stress regulates Notch target gene expression via
           transforming growth factor-β signaling in murine pre-osteoblast cell line
    • Authors: Jeeranan Manokawinchoke; Prasit Pavasant; Thanaphum Osathanon
      Pages: 47 - 54
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Jeeranan Manokawinchoke, Prasit Pavasant, Thanaphum Osathanon
      Objective Different mechanical stimuli regulate behaviors of various cell types, including osteoblasts, osteocytes, and periodontal ligament fibroblasts. Notch signaling participates in the mechanical stress-regulated cell responses. The present study investigated the regulation of Notch target gene and sclerostin (Sost) expression in murine pre-osteoblast cell line (MC3T3-E1) under intermittent compressive stress. Methods MC3T3-E1 were subjected to the intermittent compressive force under the computerized controlled machine. In some experiments, cells were pretreated with chemical inhibitors for Notch and transforming growth factor (TGF)-β signaling prior to mechanical stimuli. To evaluate role of Notch signaling in MC3T3-E1 cells under unloaded condition, cells were seeded on indirect immobilized Notch ligand (Jagged1). Gene expression was determined using real-time quantitative polymerase chain reaction. Results The intermittent compressive stress significantly upregulated Notch target gene expression (Hes Family BHLH transcription factor 1; Hes1 and Hairy/enhancer-of-split related with YRPW motif protein1; Hey1). The intermittent stress-induced Hes1 and Hey1 mRNA expression could be inhibited by a γ-secretase inhibitor (DAPT) or a TGF-β superfamily type I activing receptor-like kinase receptors inhibitor (SB431542). The results imply that intermittent compressive stress regulates Notch signaling via TGF-β pathway. Further, the intermittent compressive stress reduced Sost mRNA expression and this phenomenon could be rescued by a DAPT pretreatment, implying the involvement of Notch signaling. However, activation of Notch signaling under the unloaded condition resulted in the increase of Sost expression and the reduction of osteogenic marker genes. Conclusions These results imply the involvement of Notch signaling in the homeostasis maintaining of osteogenic cells under mechanical stress stimuli.

      PubDate: 2017-06-12T05:29:59Z
      DOI: 10.1016/j.archoralbio.2017.05.020
      Issue No: Vol. 82 (2017)
  • Effect of 1.2% of simvastatin gel as a local drug delivery system on
           Gingival Crevicular Fluid interleukin-6 & interleukin-8 levels in non
           surgical treatment of chronic periodontitis patients
    • Authors: Gayathri Gunjiganur Vemanaradhya; Shilpa Emani; Dhoom Singh Mehta; Shilpy Bhandari
      Pages: 55 - 61
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Gayathri Gunjiganur Vemanaradhya, Shilpa Emani, Dhoom Singh Mehta, Shilpy Bhandari
      Aim The present study was carried out to evaluate the effect of 1.2% simvastatin gel as local drug delivery (LDD) system on Gingival Crevicular Fluid (GCF) Interleukin -6 (IL-6) and Interleukin-8 (IL-8) levels in chronic periodontitis patients, in addition to scaling and root planing (SRP). Methods A total of 46 chronic periodontitis patients were equally divided into two groups. Group I patients were treated by SRP; Group II patients were treated by SRP followed by LDD of 1.2% simvastatin (SMV) gel. Plaque index (PI), Gingival index(GI), Sulcus Bleeding Index (SBI), Probing pocket depth (PPD) and Relative clinical attachment level (CAL) were recorded & GCF samples were collected at baseline (0day) and at 45th day from both the groups. The collected GCF samples were analysed for IL-6 and IL-8 levels with enzyme-linked immunosorbent assay (ELISA). Results Both the groups showed significant reduction in all the clinical parameters scores and IL-6 and IL-8 levels after non-surgical periodontal therapy (SRP for group I/SRP+1.2% SMV gel for group II) in contrast to baseline values. However, a greater reduction was observed in group II. A non-significant positive correlation was observed between clinical parameters and IL-6 and IL-8 levels except at baseline, a significant correlation was observed between PPD &IL 6 levels in group II. Conclusions In adjunct to SRP, 1.2% Simvastatin gel acts as an effective local drug delivery agent for the management of chronic periodontitis.

      PubDate: 2017-06-12T05:29:59Z
      DOI: 10.1016/j.archoralbio.2017.05.022
      Issue No: Vol. 82 (2017)
  • Effect of interocclusal appliance on bite force, sleep quality, salivary
           cortisol levels and signs and symptoms of temporomandibular dysfunction in
           adults with sleep bruxism
    • Authors: João Vicente Rosar; Taís de Souza Barbosa; Ilo Odilon Villa Dias; Fernanda Yukie Kobayashi; Yuri Martins Costa; Maria Beatriz Duarte Gavião; Leonardo Rigoldi Bonjardim; Paula Midori Castelo
      Pages: 62 - 70
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): João Vicente Rosar, Taís de Souza Barbosa, Ilo Odilon Villa Dias, Fernanda Yukie Kobayashi, Yuri Martins Costa, Maria Beatriz Duarte Gavião, Leonardo Rigoldi Bonjardim, Paula Midori Castelo
      Objective The purpose was to evaluate the effect interocclusal appliance therapy on bite force (BF), sleep quality and salivary cortisol levels in adults with SB diagnosed by polysomnography. As a secondary aim, signs and symptoms of temporomandibular dysfunction (TMD) were evaluated. Design Forty-three adults (19–30 y/o) were divided into two groups: experimental group (GSB), composed of 28 subjects with SB, and control group (GC), without SB and TMD (n=15). GSB was treated with stabilization interocclusal splint and evaluated at time intervals: before (baseline), one month (T1) and two months (T2) after therapy began, to collect data related to BF, sleep quality (Pittsburgh Sleep Quality Index), salivary cortisol levels and TMD. GC was also examined three times and received no therapy. Data were analysed by means of normality tests, t-test/Mann-Whitney and One-way ANOVA repeated measures (Tukey post-test). Two-way ANOVA test for repeated measures was applied to verify the effect time*group interaction on the variance of each dependent variable (α=0.05). Results GSB showed an increase in BF and a positive effect on muscular symptomatology, range of mandibular movements and sleep quality; in GC these parameters did not differ. Cortisol concentration decreased between baseline and T1 in GSB (F(1,31) =4.46; test power=62%; p=0.017). The variance observed for BF, TMD and sleep quality among time points was dependent on the group (moderate effect size: partial Eta square >0.16; test power >80%). Conclusions The results suggested that short-term interocclusal appliance therapy had a positive effect on BF, temporomandibular symptomatology, sleep quality and salivary cortisol levels in adults with SB.

      PubDate: 2017-06-12T05:29:59Z
      DOI: 10.1016/j.archoralbio.2017.05.018
      Issue No: Vol. 82 (2017)
  • Developmental mechanism of muscle–tendon–bone complex in the
           fetal soft palate
    • Authors: Michiyuki Nara; Kei Kitamura; Masahito Yamamoto; Ryotaro Nagakura; Keisuke Mitomo; Satoru Matsunaga; Shinichi Abe
      Pages: 71 - 78
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Michiyuki Nara, Kei Kitamura, Masahito Yamamoto, Ryotaro Nagakura, Keisuke Mitomo, Satoru Matsunaga, Shinichi Abe
      Objective This study was performed to investigate how the palatine aponeurosis, medial pterygoid process (MPP) of the sphenoid bone, and tensor veli palatini (TVP) muscle form the pulley: muscle–tendon–bone complex. Design Mice at embryonic day (ED) 14–17 were used as sample in this study. Azan staining was performed to observe the morphology, and immunohistochemical staining of desmin was performed to closely observe the development of the myotendinous junction. To confirm the bone formation process, immunohistochemical staining of type II collagen (col II), tartrate-resistant acid phosphatase (TRAP), and alkaline phosphatase (ALP) staining were performed. Furthermore, to objectively evaluate bone formation, the major axis and width of the MPP were measured, and osteoclasts that appeared in the MPP were counted. Results At ED 14 and 14.5, ALP showed a reaction throughout the MPP. The col II-positive area expanded until ED 16.5, but it was markedly reduced at ED 17. The TVP initially contacted with the palatine aponeurosis at ED 16.5. The major axis and width of the MPP and the number of TRAP-positive osteoclasts were significantly increased as the TVP and palatine aponeurosis joined. Conclusions Therefore, in addition to the tissue units: muscle, tendon, and bone, the interaction in organogenesis promotes rapid growth of the pulley: muscle–tendon–bone complex.

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.06.001
      Issue No: Vol. 82 (2017)
  • Early bony changes associated with bisphosphonate-related osteonecrosis of
           the jaws in rats: A longitudinal in vivo study
    • Authors: Josy Lorena Peres Vilarinho; Nathália Ferrare; Andreia Maria Rocha Moreira; Helora Freitas Moura; Ana Carolina Acevedo; Sacha Braun Chaves; Nilce Santos Melo; André Ferreira Leite; Sérgio Bruzadelli Macedo; Melissa Paoletti de Souza; Ana Tereza Bittencourt Guimarães; Paulo Tadeu Figueiredo
      Pages: 79 - 85
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Josy Lorena Peres Vilarinho, Nathália Ferrare, Andreia Maria Rocha Moreira, Helora Freitas Moura, Ana Carolina Acevedo, Sacha Braun Chaves, Nilce Santos Melo, André Ferreira Leite, Sérgio Bruzadelli Macedo, Melissa Paoletti de Souza, Ana Tereza Bittencourt Guimarães, Paulo Tadeu Figueiredo
      Objective To evaluate early bony changes in an animal model of Medication-Related Osteonecrosis of the Jaw (MRONJ) at the side of the local trauma and at the contralateral side, comparing with a control group. Bony changes were evaluated by Microcomputed Tomography (MicroCT) at three times points: at baseline (T0), after drug administration (T1) and after dental extraction (T2). Design Two groups were compared: the experimental group in which zoledronic acid (ZA) was administered (17 rats) and the control group (13 rats). Dental extractions of the lower left first molars were performed in all animals. The left side was considered as the supposed affected area in the ZA group, and the right side was considered as the unaffected area. In these areas, the following structural microtomographic bone parameters were calculated: Bone Mineral Density (BMD), Trabecular Thickness (Tb.Th), and Bone Volume Proportion (BV/TV). The comparison of quantitative bone parameters among the different sides and experimental phases of both studied groups were performed by ANOVA-factorial. Results None of the animals of the control group developed MRONJ. In the ZA group, 76% presented bone exposure. From T0 to T1, Tb.Th and BV/TV increased, and in T2, the mean values were higher in ZA group than in the control group. BMD increased throughout the different phases of both groups. Conclusions Structural bony changes occurred in the ZA group at both mandibular sides before the dental extraction (T1). Tb.Th and BV/TV should be further investigated as potential early bone markers of MRONJ.

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.06.002
      Issue No: Vol. 82 (2017)
  • Immunological and epidemiological factors affecting candidiasis in HIV
           patients beginning antiretroviral therapy in an Asian clinic
    • Authors: Endah A.T. Wulandari; Henny Saraswati; Robiatul Adawiyah; Samsuridjal Djauzi; Retno Wahyuningsih; Patricia Price
      Pages: 86 - 91
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Endah A.T. Wulandari, Henny Saraswati, Robiatul Adawiyah, Samsuridjal Djauzi, Retno Wahyuningsih, Patricia Price
      Objectives Oropharyngeal candidiasis (OPC) is common in HIV patients beginning antiretroviral therapy (ART). Here we address the response to ART, and the roles of poor oral hygiene and defects in local innate immunity with a focus on salivary β-defensins, as they are implicated in control of candidiasis but have not been investigated in this context. Design ART naïve HIV-infected adults (n=82) with <200 CD4+ T-cells/mm3 attending clinics at Cipto Mangunkusumo Hospital, Jakarta, were examined at the commencement of ART, and 73 were re-examined after 3 months. OPC was detected by clinical examination, and Candida albicans and fungal burdens were determined following culture on CHROMagar and saboroud-dextrose agar (resp). Salivary β-defensins (−2 and −3) were quantified by ELISA. Healthy control subjects (n=40) matched the patients by age and gender. Results OPC was evident in 47 patients before ART, and associated with greater fingal burdens. No OPC was detected in healthy controls and culture positivity was rare. ART decreased the prevalence of OPC to 8/73 HIV patients re-examined after 3 months, with reduced total fungal and C. albicans burdens. The incidence of OPC was independent of oral hygiene. Hyposalivation was more common in untreated HIV patients (16%) than after 3 months on ART and was rare in healthy controls. HIV patients were also more likely to have acidic saliva. Salivary β-defensin-2 was elevated in the presence of C. albicans pseudohyphae and OPC after 3 months on ART, but β-defensin-3 was not affected by OPC or ART. Conclusions ART reduces the prevalence of OPC, and the total fungal and C. albicans burden. Levels of salivary β-defensin-2 may associate with OPC in HIV patients responding to ART.

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.05.021
      Issue No: Vol. 82 (2017)
  • Effect of gels containing chlorhexidine or epigallocatechin-3-gallate on
           the protein composition of the acquired enamel pellicle
    • Authors: Cíntia Maria de Souza-e-Silva; Talita Mendes da Silva Ventura; Luiza de Pau; la Silva Cassiano; Aline de Lima Leite; Marília Afonso Rabelo Buzalaf
      Pages: 92 - 98
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Cíntia Maria de Souza-e-Silva, Talita Mendes da Silva Ventura, Luiza de Pau, la Silva Cassiano, Aline de Lima Leite, Marília Afonso Rabelo Buzalaf
      Objective This study evaluated changes in protein profile of the acquired enamel pellicle (AEP) formed in vivo, after application of gels containing chlorhexidine or EGCG and further challenge with citric acid. Design AEP was formed in 9 volunteers for 2h and then treated with one of the following gels: placebo, 400μM EGCG or 0.012% chlorhexidine. A thin layer of gel was applied and after 1min the excess was removed. One hour after gel application, the AEP was collected from the buccal surface (upper and lower jaw) of one of the sides with filter paper dipped in 3% citric acid. On the other side, erosive challenge was performed through gentle application of 1% citric acid (pH 2.5) for 20s (using a pipette) followed by washing with deionized water. The AEP was collected as mentioned before. Proteomic analysis was performed through liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI–MS/MS). The MS/MS spectra obtained were compared with human protein databases (SWISS-PROT). Label-free quantitation was done using the PLGS software. Results In total, 223 proteins were identified. After treatment with EGCG and CHX gels, proteins with potential functions to protect against caries and erosion such as PRPs, calcium-bind proteins and Statherin were increased. When EGCG and CHX-treated AEPs were challenged with citric acid, there was increase in cystatins and Profilin-1. Conclusion CHX- and EGCG-treated AEPs, submitted to challenge with citric acid or not, had remarkable changes in their proteomic profiles.

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.05.024
      Issue No: Vol. 82 (2017)
  • Microcalcifications in stone-obstructed human submandibular gland are
           associated with apoptosis and cell proliferation
    • Authors: Ivan Lau; Ajay Potluri; Cliff-Lawrence Ibeh; Robert S. Redman; Edina Paal; Bidhan C. Bandyopadhyay
      Pages: 99 - 108
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Ivan Lau, Ajay Potluri, Cliff-Lawrence Ibeh, Robert S. Redman, Edina Paal, Bidhan C. Bandyopadhyay
      Objective Human submandibular gland (SMG) stones are associated with inflammation, fibrosis and microcalcifications in the surrounding tissues. However, there is little information about the accompanying cell injury-repair process, apoptosis, and cell proliferation. The purpose of this study was to investigate such an association and its clinical significance. Design of study Mid-gland paraffin sections of human SMGs (“stone glands”) and normal SMGs (“non-stone glands”) were subjected to stains for general histology (hematoxylin and eosin), fibrosis (Masson’s trichrome), and calcification (alizarin red) and to immunohistochemistry for proliferative activity (Ki-67), and apoptosis (Caspase-3). Tissues were assessed for areas of inflammation, calcium deposition, and fibrosis, and for cycling and apoptotic cells. Results Acini were atrophic and proportionately fewer in lobules with fibrosis in stone glands. Additionally, stone glands had intraluminal calcifications (microliths) in scattered excretory and striated ducts and blood vessel walls. Areas of inflammation and fibrosis were small and uncommon, and calcifications were not seen in non-stone glands. Proliferating and apoptotic cells were common in the main duct of stone glands where ciliated and mucous cell hyperplasia and stratified squamous metaplasia had occurred, uncommon in the main duct of non-stone glands, and uncommon in all other parenchymal elements of both stone and non-stone glands. Conclusion Stone obstruction in the main excretory ducts of SMG resulted in progressive depletion of acini from proximal to distal lobules via calcification, inflammation, fibrosis, and parenchymal cell atrophy, apoptosis and proliferation. Interlobular duct microliths contributed to this depletion by further provoking intralobular inflammation, fibrosis, and acinar atrophy.
      Graphical abstract image

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.05.001
      Issue No: Vol. 82 (2017)
  • Toll-like receptor 2 rs4696480 polymorphism and risk of oral cancer and
           oral potentially malignant disorder
    • Authors: Camila de Barros Gallo; Xabier Marichalar-Mendia; Amaia Setien-Olarra; Amelia Acha-Sagredo; Naiara Garcia Bediaga; Maria Luisa Gainza-Cirauqui; Norberto Nobuo Sugaya; Jose Manuel Aguirre-Urizar
      Pages: 109 - 114
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Camila de Barros Gallo, Xabier Marichalar-Mendia, Amaia Setien-Olarra, Amelia Acha-Sagredo, Naiara Garcia Bediaga, Maria Luisa Gainza-Cirauqui, Norberto Nobuo Sugaya, Jose Manuel Aguirre-Urizar
      Objectives The aim of this study was to identify the possible association between TLR polymorphisms and an increased risk of developing head and neck cancer, including oral (OSCC) and laryngeal squamous cell carcinoma (LSCC), and oral potentially malignant disorders, such as oral lichenoid disease (OLD), including oral lichen planus (OLP) and oral lichenoid lesions (OLL). Design This case-control study included 40 OSCC, 35 LSCC, 175 OLD (129 OLP and 46 OLL) patients and 89 healthy controls, all of them from the Basque Country, Spain. Genetic polymorphisms in TLR1, TLR2, TLR4, TLR6, TLR9, and TLR10 were genotyped by TaqMan® assays or pyrosequencing. Results The chi-square analysis showed that the variant A of the SNP TLR2-rs4696480 polymorphism significantly increased the risk of OSCC (p=0.03) and OLL (p=0.02). Conclusions The TLR2-rs4696480 polymorphism may be relevant to OSCC and OLL susceptibility in this population encouraging further studies on the TLR2 pathway and its possible association with this group of oral potentially malignant disorders and oral cancer. This may also prove the use of TLR polymorphisms as risk markers for oral and laryngeal cancer.

      PubDate: 2017-06-16T05:39:13Z
      DOI: 10.1016/j.archoralbio.2017.06.003
      Issue No: Vol. 82 (2017)
  • Metabolic control and periodontal treatment decreases elevated oxidative
           stress in the early phases of type 1 diabetes onset
    • Authors: Cüneyt A. Aral; Özlem Nalbantoğlu; Bilge G. Nur; Mustafa Altunsoy; Kübra Aral
      Pages: 115 - 120
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Cüneyt A. Aral, Özlem Nalbantoğlu, Bilge G. Nur, Mustafa Altunsoy, Kübra Aral
      Objective Recently, increasing concern has been focused on the contribution of oxidative stress in the pathology of periodontal disease and diabetes mellitus. Firstly, the present study aimed to analyze gingival crevicular fluid (GCF), salivary, and serum oxidative status in children with type 1 diabetes mellitus (T1DM) at diagnosis and systemically healthy children with and without gingivitis. Additionally, the diabetic patients were reevaluated after diabetes and periodontal treatment. Design The study groups were composed of 32 T1DM patients at diagnosis, and age- and gender-matched thirty-six systemically healthy children with (G) and without (H) gingivitis. The diabetic patients who took insulin therapy (1.5 units/kg/day totally) and periodontal treatment (oral hygiene education with professional scaling) were reevaluated after 3 months. The levels of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were recorded. Results GCF, salivary, and serum OSI were elevated in group T1DM compared to the other groups at baseline (p<0.05), and decreased in group T1DM at reevaluation compared to baseline (p<0.05). GCF OSI was positively correlated with periodontal clinical parameters (p<0.05). Glycated hemoglobin was positively correlated with GCF TOS (r=0.302, p=0.007), GCF OSI (r=0.346, p=0.002), salivary TOS (r=0.326, p=0.046), and serum TOS (r=0.239, p=0.044). Conclusion The instability in the oxidative status that accompanies diabetes may be considered a significant pathogenic factor of diabetes-related periodontal inflammation.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.009
      Issue No: Vol. 82 (2017)
  • Mucoepidermoid carcinoma-associated expression of MUC5AC, MUC5B and
           mucin-type carbohydrate antigen sialyl-Tn in the parotid gland
    • Authors: Johannes H. Matse; Wiresh K. Bharos; Enno C.I. Veerman; Elisabeth Bloemena; Jan G.M. Bolscher
      Pages: 121 - 126
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Johannes H. Matse, Wiresh K. Bharos, Enno C.I. Veerman, Elisabeth Bloemena, Jan G.M. Bolscher
      Objectives The aberrant expression of mucins and mucin-type carbohydrates has been described in many types of cancer, including mucoepidermoid carcinoma (MEC), a malignant salivary gland tumor. In this study, we examined the aberrant expression patterns of mucins (MUC1, MUC4, MUC5AC and MUC5B), simple mucin-type carbohydrate antigens (Tn, sialyl-Tn and T) and mature carbohydrate antigens (Lewisa and sulfo-Lewisa antigens) in MEC originating from the parotid gland, which normally does not secrete mucins. Design We conducted an immunohistochemical study to investigate the presence of mucins and carbohydrates in 24 MEC samples originating from the parotid gland and in surrounding normal tissue of the same gland in comparison 6 samples of normal salivary glands. The expression levels were compared with respect to the histological grading. Furthermore, 24 MEC samples from non-parotid salivary glands were included. Results We observed loss of topology of membrane-bound MUC1 and MUC4, and de novo expression of MUC5AC, MUC5B and sialyl-Tn in MEC that originated in the parotid gland. Furthermore, mucins MUC1, MUC4 and carbohydrate antigens Tn, sialyl-Tn, T, Lewisa and sulfo-Lewisa were overexpressed in MEC samples compared to surrounding normal salivary gland tissues. MUC1 was expressed in both low- and high grade MECs, whereas MUC4 was not expressed in high grade MECs of the parotid gland. Conclusion During the development of MEC in the parotid gland, the genes for gel-forming secretory mucins are switched on. Besides these MEC tissues overexpress short oligosaccharides, suggesting that the glycosylation machinery is altered.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.010
      Issue No: Vol. 82 (2017)
  • Stimulatory effect of Aggregatibacter actinomycetemcomitans DNA on
           proinflammatory cytokine expression by human gingival fibroblasts
    • Authors: Uriel Soto-Barreras; Gabriela Cortés-Sandoval; Ruben Dominguez-Perez; Alejandra Loyola-Leyva; Panfilo-Raymundo Martinez-Rodriguez; Juan Pablo Loyola-Rodriguez
      Pages: 127 - 133
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Uriel Soto-Barreras, Gabriela Cortés-Sandoval, Ruben Dominguez-Perez, Alejandra Loyola-Leyva, Panfilo-Raymundo Martinez-Rodriguez, Juan Pablo Loyola-Rodriguez
      Objective While different virulence factors have been reported of Aggregatibacter actinomycetemcomitans (Aa), there is little information about the stimulatory effect of its DNA. The main purpose of this study was to assess the inflammatory response of human gingival fibroblasts (HGFs) stimulated with A. actinomycetemcomitans DNA. Design Cytokine levels of IL-6, IL-1α and TNF-α were measured on the supernatant of HGFs activated with 10, 25, 50 and 100μg/ml DNA of Aa during 24h. Primary cultures of HGFs were infected with Aa and its DNA at different times and concentrations to compare its cytotoxic effect. Cell damage and adhesion of Aa to HGFs were evaluated under light microscopy and Scanning electron microscopy respectively. Results There was a statistical difference (p< 0.05) in cytokine expression in HGFs activated by bacterial DNA with a dose dependent on IL-6 expression and a significantly elevated expression of IL-1α and TNF-α compared to Human DNA negative control. Substantial morphological alterations were observed after infection of A. actinomycetemcomitans in HGFs but not with bDNA exposure. Aggregatibacter actinomycetemcomitans showed a high rate of adhesion and cell damage to HGFs after 30min. Conclusions Genomic DNA of A. actinomycetemcomitans could be a factor in the pathogenesis of periodontitis that might play a major role in the inflammatory response.
      Graphical abstract image

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.016
      Issue No: Vol. 82 (2017)
  • Quantitative study of the proportion of the pore volume of human fluorotic
           enamel filled by resin infiltrant
    • Authors: Frederico Barbosa de Sousa; Isabel Maria Porto Lelis; Regina Célia Bressan Queiroz Figueiredo; Andressa Cavalcanti Pires; Raquel Fernanda Gerlach
      Pages: 134 - 140
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Frederico Barbosa de Sousa, Isabel Maria Porto Lelis, Regina Célia Bressan Queiroz Figueiredo, Andressa Cavalcanti Pires, Raquel Fernanda Gerlach
      Aim Capillarity theory predicts that the pore volume infiltrated by a liquid in a body with tubular capillaries is directly proportional to the capillary radius. The expected volume available for infiltration is the loosely bound water volume, which can be related to the capillary radii. We tested the hypothesis that the proportion of the pore volume infiltrated by resin infiltrant ( V r a t i o r e sin ) is correlated and agrees with the proportion of the pore volume with loosely bound water ( V r a t i o a 2 ). Design Seven human fluorotic third molars (4 unerupted and 3 erupted; TF scores 4 to 7; fluoride content of inner coronal dentin ranged from 143 to 934μg Fluoride/g) were prepared and resin infiltration was performed during 10min in fluorotic enamel ground sections. Penetration depths were measured (polarizing microscopy and CLSM) and mineral volume and non-mineral volumes were measured at histological points (n=92) along transversal lines traced from the enamel surface to the enamel-dentin junction. Results No well-mineralized surface layer was found. Infiltration depths ranged from 250μm to 900μm. V r a t i o r e sin ranged from 1.8 to 17.7% (mean of 10.13%±4.1%), was lower than V r a t i o a 2 (p<0 Hedge’s g=1.51, 95% CI: 1.18/1.83), and correlated positively with V r a t i o a 2 (R=0.684; 95% CI: 0.557/0.780) and negatively with the air volume remained after infiltration (R=−0.79; 95% CI: −0.698/−0.780). V r a t i o a 2 exceeded V r a t i o r e sin in 5% (1/4 of V r a t i o a 2 ) on average. Conclusion V r a t i o a 2 and V r a t i o r e sin correlated well, but lacked good agreement. Organic matter, firmly bound water and air remained in enamel pores after resin infiltration.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.017
      Issue No: Vol. 82 (2017)
  • Potential chemotherapeutic effects of diosgenin, zoledronic acid and
           epigallocatechin-3-gallate on PE/CA-PJ15 oral squamous cancer cell line
    • Authors: Eduardo Pons-Fuster López; Qin-tong Wang; Wei Wei; Pia López Jornet
      Pages: 141 - 146
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Eduardo Pons-Fuster López, Qin-tong Wang, Wei Wei, Pia López Jornet
      Objective To study the potential chemotherapeutic effects of Diosgenin, zoledronic acid and Epigallocatechin-3-gallate on oral squamous cell cancer (OSCC). Materials and methods Cell viability, migration, apoptosis and cell cycle evaluation assays were performed in order to assess the effects of different doses of Diosgenin, zoledronic acid and Epigallocatechin-3-gallate on the PE/CA-PJ15 cell line. Results Doses of 100μM of diosgenin or zoledronic acid reduced cell viability significantly after 72h (p<0.001), as well as increasing apoptosis (p<0.05 and p<0.01 respectively). All three agents reduced cell migration and altered the cell cycle, each at a different phase of the cycle. Conclusion while DG and ZA reduced cell viability, increased apoptosis, inhibited cell migration and modified the cell cycle in different ways, EGCG only modified the cell cycle and reduced cell migration. These agents present a potential chemotherapeutic effect on PE/CA-PJ15 OSSC cell line, which have to be further studied.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.05.023
      Issue No: Vol. 82 (2017)
  • Interferential effect of the over-erupted third molar on chewing movement
    • Authors: Shaoxiong Guo; Baoyong Li; Kun Qi; Mian Zhang; Han Zhang; Donghui Guo; Xiaodong Liu; Meiqing Wang
      Pages: 147 - 152
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Shaoxiong Guo, Baoyong Li, Kun Qi, Mian Zhang, Han Zhang, Donghui Guo, Xiaodong Liu, Meiqing Wang
      Objective To clarify whether over-eruption of the mandibular third molar can disturb chewing movement. Methods Eighteen patients with a unilateral mandibular over-erupted third molar confirmed by both study cast observation and T-scan occlusal detection were selected from a sample of patients with complaints of temporomandibular disorder (TMD) symptoms. A unilateral gum-chewing trace was recorded separately for left and right side chewing by an electrognathography system. The average chewing pattern (ACP) was created based on segments from the recorded chewing trace to represent the chewing movement characteristics of each individual. Two factors, the TMD symptomatic side and the over-eruption side, were analysed for their effects on values of difference in the parameters (Δvalue) regarding the ACP between chewing with right and left side. Three-dimensional amplitudes of ACP and the cross point value of ACP with the vertical axis (termed the cross zero point value) which described the turning point of the chewing cycle from the balancing side to the working side, were compared between sides. Results The over-eruption side had an effect on the Δvalue of the medial amplitude, the lateral amplitude, and the cross zero point (P<0.05), but the symptomatic side didn’t (P>0.05). When chewing on the over-eruption side, the medial amplitude was shorter, the lateral amplitude was larger, and the cross zero point value was smaller than those when chewing on the other side (P<0.05). Conclusion The present data indicate an effect of the over-erupted mandibular third molar on the chewing pattern while that from the symptom(s) is limited.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.012
      Issue No: Vol. 82 (2017)
  • Co-localization of endogenous Arf6 and its activator EFA6D in the granular
           convoluted tubule cells of mouse submandibular glands under normal
           conditions and when stimulated by isoproterenol, noradrenaline and
    • Authors: Apussara Tachow; Wipawee Thoungseabyoun; Laorrat Phuapittayalert; Kanoktip Petcharat; Hiroyuki Sakagami; Hisatake Kondo; Wiphawi Hipkaeo
      Pages: 153 - 159
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Apussara Tachow, Wipawee Thoungseabyoun, Laorrat Phuapittayalert, Kanoktip Petcharat, Hiroyuki Sakagami, Hisatake Kondo, Wiphawi Hipkaeo
      Objective This study proposed to investigate the localization at light and electron microscopic levels of Arf6 and its activator EFA6D in the mouse submandibular gland (SMG) under normal conditions and when stimulated by adrenergic or cholinergic agonists. Materials and methods SMGs of male adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and noradrenalin were used as adrenergics, while carbachol was used for the cholinergic stimulant. SMGs were examined at 15, 30, 60 and 120min after intraperitoneal injection of these agents. Results Immunoreactivities for both Arf6 and its activator EFA6D were similarly intense in the basolateral domain of GCTs, but no significant immunoreactivities were seen in the apical domain of GCT cells or any domain of acinar cells under normal conditions. In immuno-electron microscopy, the immunoreactive materials were mainly deposited on the basolateral plasma membranes and subjacent cytoplasm. Shortly after injection of isoproterenol and noradrenaline, but not carbachol, the immunoreactivities for both molecules were additionally seen on the apical plasmalemma of most, if not all, GCT cells, but not acinar cells. Conclusion The present findings suggest that the direct involvement of Arf6/EFA6D in regulatory exocytosis at the apical plasma membrane of acinar and GCT cells is apparently to be smaller, if present, than that of endocytosis at the basolateral membranes of GCT cells under normal conditions. This also suggests that the two molecules function additionally at the apical membrane of GCT cells for modulation of saliva secretion under β-adrenoceptor stimulation.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.007
      Issue No: Vol. 82 (2017)
  • Expression of p63 and CD44 in oral squamous cell carcinoma and correlation
           with clinicopathological parameters
    • Authors: Nasrollah Saghravanian; Kazem Anvari; Narges Ghazi; Bahram Memar; Maryam Shahsavari; Monavar Afzal Aghaee
      Pages: 160 - 165
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Nasrollah Saghravanian, Kazem Anvari, Narges Ghazi, Bahram Memar, Maryam Shahsavari, Monavar Afzal Aghaee
      Squamous cell carcinoma (SCC) is the sixth most frequent malignant tumor of the head and neck region. Despite advances in therapeutic options over the last decades, the rate of mortality and morbidity has not been improved markedly. A small subset of cells, cancer stem cells (CSCs), with self-renewal properties have become a major focus of current cancer research. CD44 and p63 are identified as candidate stem cell markers in normal squamous epithelium and SCC. The role of these markers in oral squamous cell carcinoma (OSCC) is still debatable. The aim of this study was to evaluate immunohistochemical expression of these markers in OSCC samples and also correlates the expression of these markers with some clinicopathological parameters of prognostic significance including histological grading, TNM staging, overall survival (OS) rate as well as patients’ age, gender, and tumor location. CD44 and p63 were expressed in all studied lesions with different degrees. Statistically significant difference was observed between CD44 and p63 expression with tumor grade and stage with higher expression in high grade and advanced OSCCs. No significant relationship was detected between markers immunoreactivity and patients age, gender, tumor location as well as OS. These markers can possibly advance our understanding of the initiating mechanisms and pathogenesis of OSCC and also result in novel therapeutic target in cancer treatment.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.011
      Issue No: Vol. 82 (2017)
  • Antibacterial effect of genetically-engineered bacteriophage
           ϕEf11/ϕFL1C(Δ36)PnisA on dentin infected with antibiotic-resistant
           Enterococcus faecalis
    • Authors: Justine Monnerat Tinoco; Nadia Liss; Hongming Zhang; Roni Nissan; Wanda Gordon; Eduardo Tinoco; Luciana Sassone; Roy Stevens
      Pages: 166 - 170
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Justine Monnerat Tinoco, Nadia Liss, Hongming Zhang, Roni Nissan, Wanda Gordon, Eduardo Tinoco, Luciana Sassone, Roy Stevens
      Objective Enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30–89% of teeth with postendodontic treatment failures. E. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. Different E. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic infections. The aim of this study was to evaluate the efficacy of a genetically engineered bacteriophage to disinfect dentin infected with antibiotic resistant strains of E. faecalis. Methods Extracted human dentin root segments were cemented into sealable two-chamber devices, fabricated from syringe needle caps to form in vitro infected-dentin models. The models were inoculated with an overnight suspension of either E. faecalis V583 (vancomycin resistant strain) or E. faecalis JH2-2 (fusidic acid and rifampin resistant, vancomycin sensitive strain). After 7days of incubation at 37°C, a suspension of a genetically engineered phage, ϕEf11/ϕFL1C(Δ36)PnisA, was added to the root canal of each infected dentin segment, and the incubation was continued for an additional 72-h. Dentin was harvested from the walls of each root canal and assayed for the residual titer of E. faecalis cells. Results The recovered E. faecalis titer was reduced by 18% for the JH2-2 infected models, and by 99% for the V583 infected models. Conclusion Treatment: of E. faecalis-infected dentin with bacteriophage ϕEf11/ϕFL1C(Δ36)PnisA consistently resulted in a decrease in the residual bacterial population of both vancomycin-sensitive and resistant strains.

      PubDate: 2017-06-22T10:42:13Z
      DOI: 10.1016/j.archoralbio.2017.06.005
      Issue No: Vol. 82 (2017)
  • Establishment of a primary culture of polymorphous low grade
           adenocarcinoma cells
    • Authors: Lucas Novaes Teixeira; Victor Angelo Martins Montalli; Silvia Borges Pimentel de Oliveira; Thais Fernanda Santos Toledo; Elizabeth Ferreira Martinez; Vera Cavalcanti de Araújo
      Pages: 188 - 193
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Lucas Novaes Teixeira, Victor Angelo Martins Montalli, Silvia Borges Pimentel de Oliveira, Thais Fernanda Santos Toledo, Elizabeth Ferreira Martinez, Vera Cavalcanti de Araújo
      Objective The aim of the present study was to establish a primary cell culture derived from polymorphous low grade adenocarcinoma (PLGA). Design The neoplastic cells were derived from a 57-year-old female patient diagnosed with PLGA. A fragment of the tumor was collected and submitted to enzymatic digestion followed by centrifugation on a Percoll gradient. The cell population was characterized by means of immunofluorescence and detection of PRKD1 gene mutations. Results Epifluorescence analysis of the primary culture revealed that the malignant epithelial cells were predominantly polygonal in shape and positive for cytokeratin 7, vimentin, and S100. The doubling time of the cell culture was 86.73h. The restriction digestion assay showed that the neoplastic cells possess PRKD1 gene mutations. Conclusion The establishment of primary cell culture derived from PLGA should be considered a useful tool for molecular analysis of this salivary gland tumor.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.015
      Issue No: Vol. 82 (2017)
  • Recovery after PILP remineralization of dentin lesions created with two
           cariogenic acids
    • Authors: K. Saeki; Y-C Chien; G. Nonomura; A.F. Chin; S. Habelitz; L.B. Gower; S.J. Marshall; G.W. Marshall
      Pages: 194 - 202
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): K. Saeki, Y-C Chien, G. Nonomura, A.F. Chin, S. Habelitz, L.B. Gower, S.J. Marshall, G.W. Marshall
      Objectives Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor (PILP) remineralization. Design Dentin blocks obtained from human third molars, divided into 6 groups (n=3). Blocks were demineralized with acetate (66h) or lactate (168h) buffer at pH 5.0 to create 140μm target lesion depths. A-DEM and L-DEM groups received no remineralization. Other groups were remineralized for 14days. 100μg/mL polyaspartate was added into the remineralizing buffer for A-PIL and L-PIL, whereas A-CAP and L-CAP were treated with the same solution but without polyaspartate. Cross-sectioned blocks were examined for shrinkage and AFM-topography. Line profiles of reduced elastic modulus (Er) were obtained by AFM-based nanoindentation across the lesion. Ultrastructures were examined with TEM. Results A-PIL and L-PIL recovered in shrinkage to the original height of the dentin and it appeared normal with tubules, with increases in Er at both outer flat and inner sloped zones. At the sloped zone, acetate lesions lost more Er but recovery rate after PILP was not statistically different from lactate lesions. A-CAP and L-CAP showed surface precipitates, significantly less recovery in shrinkage or Er as compared to PILP groups. TEM-ultrastructure of PILP groups showed similar structural and mineral components in the sloped zone for lesions produced by either acid. Conclusions The PILP process provided significant recovery of both structure and mechanical properties for artificial lesions produced with acetate or lactate.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.006
      Issue No: Vol. 82 (2017)
  • Role of proteoglycans on the biochemical and biomechanical properties of
           dentin organic matrix
    • Authors: Cristina de Mattos Pimenta Vidal; Ariene Arcas Leme-Kraus; Momina Rahman; Ana Paula Farina; Ana K. Bedran-Russo
      Pages: 203 - 208
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Cristina de Mattos Pimenta Vidal, Ariene Arcas Leme-Kraus, Momina Rahman, Ana Paula Farina, Ana K. Bedran-Russo
      Objective Proteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen. Methods Selective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α=0.05). Results Transmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p=0.0224) and control (p=0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix. Conclusions GAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.020
      Issue No: Vol. 82 (2017)
  • The effect of a hydroxyapatite impregnated PCL membrane in rat subcritical
           calvarial bone defects
    • Authors: Monica Feresini Groppo; Paulo Henrique Caria; Alexandre Rodrigues Freire; Sidney R. Figueroba; Wilson Alves Ribeiro-Neto; Rosario Elida Suman Bretas; Felippe Bevilacqua Prado; Francisco Haiter-Neto; Flavio Henrique Aguiar; Ana Claudia Rossi
      Pages: 209 - 215
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Monica Feresini Groppo, Paulo Henrique Caria, Alexandre Rodrigues Freire, Sidney R. Figueroba, Wilson Alves Ribeiro-Neto, Rosario Elida Suman Bretas, Felippe Bevilacqua Prado, Francisco Haiter-Neto, Flavio Henrique Aguiar, Ana Claudia Rossi
      Objective The present study evaluated the effect of polymeric-nanofibers membranes impregnated with microparticulate hydroxyapatite (HA) in the subcritical calvarial bone defects (SCBD) healing. Design PCL membranes with and without HA were obtained by electrospinning. SCBD were perforated (3.3mm) in left and right sides of 36 rat calvarias. The right-side SBCD of 18 animals was filled with HA mixed with blood clot and blood clot at the contralateral side. The remaining animals received PCL+HA membrane at the right-side SCBD and PCL membrane at the contralateral side. Animals were killed after 30, 60 and 90days after surgery. Bone defect volume (in mm3) was measured by tomography (CBCT). Qualitative histological analysis and SBCD area (in mm2) were measured. Quantitative data were submitted to Kruskal-Wallis/Dunn tests. Results Reduction of SBCD volume was observed in all treatments but PCL. Association with HA significantly improved bone healing induced by PCL and blood clot. PCL+HA induced the lowest SBCD volume at 60 and 90days. Complete bone healing was not observed even at 90days in SCBD treated with blood clot. In every period, more bone formation was observed for SCBD treated with membranes. Conclusions We concluded that both PCL membrane and HA were able to improve bone healing.
      Graphical abstract image

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.018
      Issue No: Vol. 82 (2017)
  • Fibulins and matrilins are novel structural components of the periodontium
           in the mouse
    • Authors: Andrea Schubert; Boris Schminke; Nicolai Miosge
      Pages: 216 - 222
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Andrea Schubert, Boris Schminke, Nicolai Miosge
      Periodontitis refers to inflammatory disease of the periodontal structures (the gingiva, dental cementum, periodontal ligament (PDL) and alveolar bone) that ultimately leads to their destruction. Whereas collagens are well-examined main components of the periodontium, little is known about the other structural proteins that make up this tissue. The aim of this study was to identify new extracellular matrix (ECM) components, including fibulins and matrilins, in the periodontium of mice. After sacrificing 14 mice (Sv/129 strain), jaws were prepared. Each tissue sample contained a molar and its surrounding alveolar bone. Immunohistochemistry was carried out on paraffin-embedded sections. Our results show that mice exhibit fibulin-3, -4 and -5 and matrilin-1, -2, -3 and -4 in PDL and in blood vessels of alveolar bone and PDL as well as in the pericellular matrix of osteocytes and cementocytes. In dental cementum, only fibulin-4 is expressed. For the first time, we show that fibulin-3, -4 and -5 and matrilin-1, -2, -3 and -4 are essential components of the periodontal tissues. Our findings indicate an association of these proteins with collagens and oxytalan fibers that might be of future interest in regenerative periodontitis therapy.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.008
      Issue No: Vol. 82 (2017)
  • Generation and characterization of a human oral squamous carcinoma cell
           line SCC-9 with CRISPR/Cas9-mediated deletion of the p75 neurotrophin
    • Authors: Ping Huang; Dongdong Tong; Jing Sun; Qing Li; Fenghe Zhang
      Pages: 223 - 232
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Ping Huang, Dongdong Tong, Jing Sun, Qing Li, Fenghe Zhang
      Objective To investigate the importance of the p75 neurotrophin receptor (p75NTR) in human tongue squamous carcinoma cells, we exploited the CRISPR/Cas9 technology to establish a p75NTR-knockout SCC-9 cell line and to explore the effect on biological functions. Materials and methods The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease (Cas9) system was used to generate genomic deletion mutants of p75NTR in the tongue squamous carcinoma cell lines SCC-9. Single-guide RNA (sgRNA) sequences were designed to target the p75NTR genomic sequence and were cloned into plasmid pGK1.1. The linearized vector was electroporated into SCC-9 cells and p75NTR deletion was confirmed using Cruiser™ enzyme digestion and PCR amplification. SCC-9 clones with successful deletion of p75NTR were identified and verified by sequencing and selected for functional testing in cell proliferation, invasion, migration, and colony-forming assays. Results Compared with control cells, p75NTR-knockout SCC-9 cells showed significantly diminished abilities to proliferate, invade, migrate, and form colonies, indicating a reduction in pro-tumorigenic behavior. Conclusion These data demonstrate, first, that the CRISPR/Cas9 system is a simplified method for generating p75NTR knockouts with relatively high efficiency, and second, that deletion of p75NTR suppresses several tumor-promoting properties of SCC-9 cells, suggesting that p75NTR is a potential target for the development of novel therapies for tongue cancer.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.004
      Issue No: Vol. 82 (2017)
  • Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue
           in elderly subjects and in vitro
    • Authors: Naho Mugita; Takayuki Nambu; Kazuya Takahashi; Pao-Li Wang; Yutaka Komasa
      Pages: 233 - 240
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Naho Mugita, Takayuki Nambu, Kazuya Takahashi, Pao-Li Wang, Yutaka Komasa
      Objective Dental plaque is a causative factor for oral disease and a potential reservoir for respiratory infection in the elderly. Therefore, there is a critical need for the development of effective methods to remove oral biofilm. The objective of this study was to investigate the effect of proteases on oral biofilm formation andremoval. Design The in vivo effect of actinidin, a cysteine protease, on the removal of tongue coating was assessed after orally taking a protease tablet. Effects of the proteases trypsin, papain and actinidin on Actinomyces monospecies biofilm and multispecies biofilm that was reconstructed using a plaque sample from the tongue coating were investigated using the microtiter plate method. Antimicrobial tests and limited proteolysis of fimbrial shaft proteins were also performed to clarify underlying mechanisms of oral biofilm removal. Results Tablets containing actinidin removed tongue coating in elderly subjects. Oral Actinomyces biofilm was significantly reduced by the proteases papain, actinidin and trypsin. Papain and trypsin effectively digested the major fimbrial proteins, FimP and FimA, from Actinomyces. Actinidin, papain and trypsin reduced multispecies biofilm that was reconstructed in vitro. Papain and trypsin inhibited formation of multispecies biofilm in vitro. Conclusions This study shows that proteases reduced oral biofilm in vivo in elderly subjects and in vitro, and suggests that protease digests fimbriae and inhibits biofilm formation.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.04.035
      Issue No: Vol. 82 (2017)
  • Excitability of the masseter inhibitory reflex after high frequency rTMS
           over the motor cortex: A study in healthy humans
    • Authors: Congcong Cui; Yuhan Song; Xiao Fan; Qian Guo; Jijun Wang; Weicai Liu
      Pages: 241 - 246
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Congcong Cui, Yuhan Song, Xiao Fan, Qian Guo, Jijun Wang, Weicai Liu
      Objective We aimed to investigate the influence of high frequency repetitive transcranial magnetic stimulation (rTMS) of the motor cortex on excitability of the masseter inhibitory reflex (MIR) in healthy subjects. Our goal in conducting this study was to obtain a better understanding of the subcortical and cortical networks related to the excitability of the central masticatory pathway. Methods Twenty healthy volunteers participated in this study. MIRs were evaluated both before and after high frequency rTMS to elicit motor evoked potentials (MEPs) using single- and double-shock techniques over the motor cortex. During measurements, the latency and duration of silent periods (SPs) with single magnetic stimulation, and the recovery of SP2 with the double-shock technique, were recorded in basal conditions and immediately after high frequency rTMS. The “real” rTMS condition consisted of 20 stimulus trains delivered at 10Hz with a 5-s stimulation duration and an intensity of 70% of the active motor threshold (AMT) over the right optimal motor spot for the masseter muscle. Results were also compared to those obtained after “sham” rTMS 1 week later Results Using the double-shock technique, SP2 recovery time was significantly shorter after high frequency rTMS compared to the basal condition (from 100 to 400ms; p<0.001), while at ISIs of 500 and 600ms, no significant effect was observed (p=0.084, p=0.948). There was also no significant change in the SP latency or duration between groups (p >0.05). Conclusions High frequency, sub-threshold rTMS of the motor cortex had a facilitative after-effect on the excitability of the MIR. This effect was likely mediated through increased cortical drive to brainstem reflex pathways, which would ultimately accelerate MIR recovery.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.014
      Issue No: Vol. 82 (2017)
  • Topical application of ointment containing 0.5% green tea catechins
           suppresses tongue oxidative stress in 5-fluorouracil administered rats
    • Authors: Hisataka Miyai; Takayuki Maruyama; Takaaki Tomofuji; Toshiki Yoneda; Tetsuji Azuma; Hirofumi Mizuno; Yoshio Sugiura; Terumasa Kobayashi; Daisuke Ekuni; Manabu Morita
      Pages: 247 - 255
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita
      Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n=28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P <0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P <0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P <0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.025
      Issue No: Vol. 82 (2017)
  • Effect of arginine on the growth and biofilm formation of oral bacteria
    • Authors: Xuelian Huang; Keke Zhang; Meng Deng; Robertus A.M. Exterkate; Chengcheng Liu; Xuedong Zhou; Lei Cheng; Jacob M. ten Cate
      Pages: 256 - 262
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Xuelian Huang, Keke Zhang, Meng Deng, Robertus A.M. Exterkate, Chengcheng Liu, Xuedong Zhou, Lei Cheng, Jacob M. ten Cate
      Background Alkali production via arginine deiminase system (ADS) of oral bacteria plays a significant role in oral ecology, pH homeostasis and inhibition of dental caries. ADS activity in dental plaque varies greatly between individuals, which may profoundly affect their susceptibility to caries. Objective To investigate the effect of arginine on the growth and biofilm formation of oral bacteria. Methods and results Polymicrobial dental biofilms derived from saliva were formed in a high-throughput active attachment biofilm model and l-arginine (Arg) was shown to reduce the colony forming units (CFU) counts of such biofilms grown for various periods or biofilms derived from saliva of subjects with different caries status. Arg hardly disturbed bacterial growth of Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis and Streptococcus gordonii in BHI medium, but only inhibited biofilm formation of S. mutans. Scanning electron microscope (SEM) showed S. mutans biofilms harboured fewer cells grown with Arg than that without Arg, even in the initial 2h and 8h phase. Confocal laser scanning microscope (CLSM) images of poly-microbial dental and S. mutans biofilms revealed the biofilms grown with Arg had lower exopolysaccharide (EPS)/bacteria ratios than those without Arg (P=0.004, 0.002, respectively). Arg could significantly reduce the production of water-insoluble EPS in S. mutans biofilms (P<0.001); however, quantitative real-time PCR (qRT-PCR) did not show significantly influence in gene expression of gtfB, gtfC or gtfD (P=0.32, 0.06, 0.44 respectively). Conclusions Arg could reduce the biomass of poly-microbial dental biofilms and S. mutans biofilms, which may be due to the impact of Arg on water-insoluble EPS. Considering the contribution to pH homeostasis in dental biofilms, Arg may serve as an important agent keeping oral biofilms healthy thus prevent dental caries.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.026
      Issue No: Vol. 82 (2017)
  • Theophylline increases saliva sonic hedgehog and improves taste
    • Authors: Robert I. Henkin; Alexandra B. Knöppel; Mona Abdelmeguid; William A. Stateman; Suzanna Hosein
      Pages: 263 - 270
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Robert I. Henkin, Alexandra B. Knöppel, Mona Abdelmeguid, William A. Stateman, Suzanna Hosein
      Objective To determine changes in saliva sonic hedgehog (Shh) and in taste dysfunction before and after oral theophylline treatment. Design Shh was measured in parotid saliva of both normal subjects and patients with taste dysfunction of multiple etiologies by use of a sensitive spectrophotometric ELISA assay. Taste dysfunction was defined clinically by both subjective inhibition of taste function (including acuity loss) and impaired gustometry. Results Theophylline treatment increased patient saliva Shh and improved taste dysfunction both subjectively and by gustometry. Conclusions By use of these systematic clinical measurements theophylline can be demonstrated to increase saliva Shh and improve taste dysfunction. These results are consistent with saliva Shh acting as a taste bud growth factor which stimulates stem cells of taste buds to initiate development and perpetuation of taste bud receptors. Measurements of saliva Shh provide an important marker for the presence of taste dysfunction and in the improvement by theophylline treatment.

      PubDate: 2017-07-01T11:02:17Z
      DOI: 10.1016/j.archoralbio.2017.06.022
      Issue No: Vol. 82 (2017)
  • Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects
           in the absence of cytotoxicity and genotoxicity
    • Authors: Jonatas Rafael de Oliveira; Daiane de Jesus Viegas; Ana Paula Réquia Martins; Cláudio Antonio Talge Carvalho; Cristina Pacheco Soares; Samira Esteves Afonso Camargo; Antonio Olavo Cardoso Jorge; Luciane Dias de Oliveira
      Pages: 271 - 279
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Jonatas Rafael de Oliveira, Daiane de Jesus Viegas, Ana Paula Réquia Martins, Cláudio Antonio Talge Carvalho, Cristina Pacheco Soares, Samira Esteves Afonso Camargo, Antonio Olavo Cardoso Jorge, Luciane Dias de Oliveira
      Objectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48h and exposed for 5min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey’s Test (P ≤0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.031
      Issue No: Vol. 82 (2017)
  • Different expression patterns of Lin28 and Lin28b in mouse molar
    • Authors: Ning Dong; Yan Liu; Tiantian Zhang; Lin Zhao; Jiangang Tian; Jianping Ruan
      Pages: 280 - 285
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Ning Dong, Yan Liu, Tiantian Zhang, Lin Zhao, Jiangang Tian, Jianping Ruan
      Objective The RNA-binding proteins Lin28 and Lin28b are expressed in many developing tissues and are involved in the biosynthesis of the microRNA let-7 family and embryogenesis processes. However, their roles in mammalian tooth development remain ill-defined. Design The spatiotemporal expressions of Lin28 and Lin28b during mouse molar odontogenesis from day E11.5 to P21 were examined through immunohistochemistry and western blot analysis. Results Both Lin28 and Lin28b were initially expressed in dental epithelium, but the expression patterns varied thereafter. Lin28 was expressed in tooth germ from early embryonic stages and was consistently expressed in the ameloblasts and odontoblasts throughout all stages of tooth development. However, positive staining of Lin28b gradually faded out with tooth germ development, before finally disappearing in tooth organ cells after birth. Conclusions These results indicate that Lin28 was spatiotemporally expressed in tooth germ throughout tooth development progression and may play an active role in ameloblast and odontoblast differentiation, as well as matrix secretion and the mineralization of enamel and dentin. Its paralogue Lin28b may have a distinct function in tooth germ formation.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.023
      Issue No: Vol. 82 (2017)
  • Chronophysiological features of the normal mineral composition of human
    • Authors: Lyudmila V. Bel’skaya; Victor K. Kosenok; Elena A. Sarf
      Pages: 286 - 292
      Abstract: Publication date: October 2017
      Source:Archives of Oral Biology, Volume 82
      Author(s): Lyudmila V. Bel’skaya, Victor K. Kosenok, Elena A. Sarf
      Background The high rate of changes in the composition of saliva can be used for the monitoring of various biorhythms in order to study the physiological characteristics of the human body. Research objective The study of the dynamics of the near-24-h mineral composition of saliva in men and women. Methods The study involved 20 men and 20 women, age 23.1±0.9years. Saliva samples were collected every 3h during the day within 10min. The mineral composition of saliva was defined by the capillary electrophoresis technique. The cross-group differences were evaluated using the non-parametric criterion. Results It was shown that the maximum values of the mineralizing capacity fall to 7–8 a.m. and 18–19p.m., which is due to the change in the rate of saliva secretion. The near-24-h dynamics of the saliva pH practically coincides with the dynamics of the Ca/P ratio; there are evident maxima at 9:00 am and 15–18p.m. The values of the Na/K ratio are out of phase with the Ca/P ratio. There is one maximum, corresponding to 3 am in the night, and one minimum at 12–13p.m., which is due to a decreased level of sodium and increased potassium concentration. Statistically valid differences between men and women in pH (p<0.001), concentrations of inorganic phosphorus (p<0.001), as well as Ca/P coefficient (p=0.011) were identified. Conclusion The dynamics of the studied parameters during 24h is characterized by pronounced intervals.

      PubDate: 2017-07-10T03:27:55Z
      DOI: 10.1016/j.archoralbio.2017.06.024
      Issue No: Vol. 82 (2017)
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