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  Subjects -> BIOLOGY (Total: 3172 journals)
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    - BIOLOGY (1511 journals)
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BIOLOGY (1511 journals)                  1 2 3 4 5 6 7 8 | Last

Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 22)
Achievements in the Life Sciences     Open Access   (Followers: 5)
ACS Synthetic Biology     Hybrid Journal   (Followers: 25)
Acta Biologica Colombiana     Open Access   (Followers: 7)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 4)
Acta Biologica Sibirica     Open Access   (Followers: 1)
Acta Biomaterialia     Hybrid Journal   (Followers: 27)
Acta Biotheoretica     Hybrid Journal   (Followers: 4)
Acta Chiropterologica     Full-text available via subscription   (Followers: 6)
acta ethologica     Hybrid Journal   (Followers: 4)
Acta Fytotechnica et Zootechnica     Open Access   (Followers: 1)
Acta Limnologica Brasiliensia     Open Access   (Followers: 3)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales     Open Access  
Acta Neurobiologiae Experimentalis     Open Access  
Acta Parasitologica     Hybrid Journal   (Followers: 10)
Acta Scientiarum. Biological Sciences     Open Access   (Followers: 2)
Acta Scientifica Naturalis     Open Access   (Followers: 3)
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis     Open Access  
Actualidades Biológicas     Open Access   (Followers: 1)
Advanced Health Care Technologies     Open Access   (Followers: 4)
Advanced Journal of Graduate Research     Open Access  
Advanced Nonlinear Studies     Hybrid Journal  
Advanced Studies in Biology     Open Access  
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 2)
Advances in Bioinformatics     Open Access   (Followers: 17)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4)
Advances in Biology     Open Access   (Followers: 9)
Advances in Biosensors and Bioelectronics     Open Access   (Followers: 7)
Advances in Cell Biology/ Medical Journal of Cell Biology     Open Access   (Followers: 25)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 12)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Ecological Research     Full-text available via subscription   (Followers: 44)
Advances in Environmental Sciences - International Journal of the Bioflux Society     Open Access   (Followers: 16)
Advances in Enzyme Research     Open Access   (Followers: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Genome Biology     Full-text available via subscription   (Followers: 8)
Advances in High Energy Physics     Open Access   (Followers: 19)
Advances in Human Biology     Open Access   (Followers: 3)
Advances in Life Science and Technology     Open Access   (Followers: 16)
Advances in Life Sciences     Open Access   (Followers: 6)
Advances in Marine Biology     Full-text available via subscription   (Followers: 18)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 23)
Advances in Organ Biology     Full-text available via subscription   (Followers: 1)
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3)
Advances in Regenerative Biology     Open Access   (Followers: 1)
Advances in Space Biology and Medicine     Full-text available via subscription   (Followers: 6)
Advances in Structural Biology     Full-text available via subscription   (Followers: 5)
Advances in Virus Research     Full-text available via subscription   (Followers: 5)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 8)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 1)
Ageing Research Reviews     Hybrid Journal   (Followers: 11)
Aging Cell     Open Access   (Followers: 16)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat     Open Access  
AJP Cell Physiology     Hybrid Journal   (Followers: 15)
AJP Endocrinology and Metabolism     Hybrid Journal   (Followers: 24)
AJP Lung Cellular and Molecular Physiology     Hybrid Journal   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
Alces : A Journal Devoted to the Biology and Management of Moose     Open Access  
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Biology Teacher     Full-text available via subscription   (Followers: 14)
American Fern Journal     Full-text available via subscription   (Followers: 1)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 8)
American Journal of Bioethics     Hybrid Journal   (Followers: 12)
American Journal of Human Biology     Hybrid Journal   (Followers: 14)
American Journal of Medical and Biological Research     Open Access   (Followers: 8)
American Journal of Plant Sciences     Open Access   (Followers: 19)
American Journal of Primatology     Hybrid Journal   (Followers: 15)
American Malacological Bulletin     Full-text available via subscription   (Followers: 3)
American Naturalist     Full-text available via subscription   (Followers: 74)
Amphibia-Reptilia     Hybrid Journal   (Followers: 6)
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Anaerobe     Hybrid Journal   (Followers: 4)
Analytical Methods     Full-text available via subscription   (Followers: 11)
Anatomical Science International     Hybrid Journal   (Followers: 3)
Animal Cells and Systems     Hybrid Journal   (Followers: 4)
Animal Models and Experimental Medicine     Open Access  
Annales de Limnologie - International Journal of Limnology     Hybrid Journal   (Followers: 1)
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3)
Annals of Applied Biology     Hybrid Journal   (Followers: 7)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 17)
Annals of Human Biology     Hybrid Journal   (Followers: 5)
Annals of Science and Technology     Open Access  
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 14)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 23)
Annual Review of Cancer Biology     Full-text available via subscription   (Followers: 2)
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 37)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 24)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 12)
Anthropological Review     Open Access   (Followers: 23)
Anti-Infective Agents     Hybrid Journal   (Followers: 3)
Antibiotics     Open Access   (Followers: 9)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 8)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 8)
Applied Bionics and Biomechanics     Open Access   (Followers: 8)
Applied Vegetation Science     Full-text available via subscription   (Followers: 10)
Aquaculture Environment Interactions     Open Access   (Followers: 4)
Aquaculture International     Hybrid Journal   (Followers: 25)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 7)
Aquatic Biology     Open Access   (Followers: 6)
Aquatic Ecology     Hybrid Journal   (Followers: 35)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 15)
Aquatic Science and Technology     Open Access   (Followers: 3)
Aquatic Toxicology     Hybrid Journal   (Followers: 23)
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 3)
Archives of Biological Sciences     Open Access  
Archives of Microbiology     Hybrid Journal   (Followers: 9)
Archives of Natural History     Hybrid Journal   (Followers: 6)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 5)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arid Ecosystems     Hybrid Journal   (Followers: 2)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 2)
Arthropods     Open Access   (Followers: 1)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 3)
Asian Bioethics Review     Full-text available via subscription   (Followers: 3)
Asian Journal of Biodiversity     Open Access   (Followers: 4)
Asian Journal of Biological Sciences     Open Access   (Followers: 3)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Developmental Biology     Open Access   (Followers: 2)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 4)
Asian Journal of Nematology     Open Access   (Followers: 4)
Asian Journal of Poultry Science     Open Access   (Followers: 4)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 6)
Autophagy     Hybrid Journal   (Followers: 3)
Avian Biology Research     Full-text available via subscription   (Followers: 4)
Avian Conservation and Ecology     Open Access   (Followers: 11)
Bacteriology Journal     Open Access   (Followers: 1)
Bacteriophage     Full-text available via subscription   (Followers: 3)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access   (Followers: 1)
Batman Üniversitesi Yaşam Bilimleri Dergisi     Open Access  
Berita Biologi     Open Access   (Followers: 1)
Between the Species     Open Access   (Followers: 1)
Bio Tribune Magazine     Hybrid Journal  
BIO Web of Conferences     Open Access  
BIO-Complexity     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bioanalytical Reviews     Hybrid Journal   (Followers: 2)
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 6)
BioCentury Innovations     Full-text available via subscription   (Followers: 1)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 16)
Biochimie     Hybrid Journal   (Followers: 7)
BioControl     Hybrid Journal   (Followers: 5)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal  
BioDiscovery     Open Access   (Followers: 2)
Biodiversitas : Journal of Biological Diversity     Open Access  
Biodiversity Data Journal     Open Access   (Followers: 4)
Biodiversity Informatics     Open Access   (Followers: 1)
Biodiversity Information Science and Standards     Open Access  
Biodiversity: Research and Conservation     Open Access   (Followers: 27)
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro     Open Access  
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 2)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 3)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
Bioethics     Hybrid Journal   (Followers: 15)
BioéthiqueOnline     Open Access  
Biofabrication     Hybrid Journal   (Followers: 4)
Biofilms     Full-text available via subscription   (Followers: 1)
Biogeosciences (BG)     Open Access   (Followers: 10)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 2)
Bioinformatics     Hybrid Journal   (Followers: 309)
Bioinformatics and Biology Insights     Open Access   (Followers: 11)
Bioinspiration & Biomimetics     Hybrid Journal   (Followers: 7)
Biointerphases     Open Access   (Followers: 1)
Biojournal of Science and Technology     Open Access  
BioLink : Jurnal Biologi Lingkungan, Industri, Kesehatan     Open Access   (Followers: 1)
Biologia     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 6)
Biological Control     Hybrid Journal   (Followers: 4)
Biological Invasions     Hybrid Journal   (Followers: 20)
Biological Journal of the Linnean Society     Hybrid Journal   (Followers: 18)
Biological Letters     Open Access   (Followers: 5)
Biological Procedures Online     Open Access  
Biological Psychiatry     Hybrid Journal   (Followers: 48)
Biological Psychology     Hybrid Journal   (Followers: 7)
Biological Research     Open Access  

        1 2 3 4 5 6 7 8 | Last

Journal Cover
Aquaculture International
Journal Prestige (SJR): 0.591
Citation Impact (citeScore): 2
Number of Followers: 25  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1573-143X - ISSN (Online) 0967-6120
Published by Springer-Verlag Homepage  [2349 journals]
  • Depth matters for bivalve culture in integrated multitrophic aquaculture
           (IMTA) and other polyculture strategies under non-eutrophic conditions
    • Authors: Carlos Sanz-Lazaro; Victoria Fernandez-Gonzalez; Pablo Arechavala-Lopez; David Izquierdo-Gomez; Elena Martinez-Garcia; Pablo Sanchez-Jerez
      Pages: 1161 - 1170
      Abstract: Bivalve cultivation, in single cultivation or in polyculture (including integrated multitrophic aquaculture; IMTA), is generally limited to eutrophic waters. We carried out a modeling study to test if, under meso- and oligotrophic conditions, depth could be a key factor for bivalve productivity associated to IMTA and other polyculture strategies. We applied the model Farm Aquaculture Resource Management (FARM) at three strata of the water column in two coastal fish farm areas in the Mediterranean Sea, using water column variables sampled seasonally to estimate the potential mussel production. According to FARM, mussel production was high in both areas and, in some cases, almost doubled when mussels were cultured below 25-m depth compared to shallower levels. Phytoplankton abundance is expected to notably influence mussel production compared to particulate organic matter. Thus, in meso- and oligotrophic stratified waters, where chlorophyll maximum is relatively deep, depth can be a key factor for the productivity of mussel cultivation. The obtained results could help to maximize the production of suspension-feeding bivalve cultivation and, therefore, the expansion and development of sustainable aquaculture in non-eutrophic marine waters.
      PubDate: 2018-10-01
      DOI: 10.1007/s10499-018-0276-9
      Issue No: Vol. 26, No. 5 (2018)
       
  • Development of a simple and rapid monoclonal antibody-based flow through
           immunogold assay (FIA) for detection of Aeromonas hydrophila
    • Authors: Abhiman Purandara Ballyaya; Moumita Mondal; Shankar Mariappa Kalkuli; Suresh Babu Padinhate Purayil
      Pages: 1171 - 1186
      Abstract: In this study, we developed a simple, low-cost, and rapid flow through immunogold assay (FIA) for detection of Aeromonas hydrophila in fish tissues and validated. The developed assay relied on colloidal gold conjugated highly specific monoclonal antibody (B8E3) against 20 kDa protein of A. hydrophila. The assay can be completed within 5 min including antigen (sample) preparation and exhibited no cross-reaction with other major aquatic pathogens such as Vibrio anguillarum, V. parahaemolyticus, V. harveyi, white spot syndrome virus (WSSV), and Aphanomyces invadans. A wider range of Aeromonas species were tested including A. caviae, A. dhakensis, A. sobria, A. veronii, A. culicicola, A. sharmana, and A. schubertii and no cross-reactivity could be observed. The developed technique FIA could detect 103 CFU/ml of aeromonad cells. In addition, the FIA is as sensitive as the first-step polymerase chain reaction (PCR) and 1000 times sensitive than the immunodot blot method. The validation study revealed the accuracy of FIA by comparing with the standard first-step PCR. The proposed FIA is low-cost, easy-to-use, and compatible for field-level analysis of A. hydrophila in fish tissues. Our developed FIA could successfully detect A. hydrophila at early stage of the infection and could be easily availed by farmers, fish producers, non-technicians, and technicians in the aquaculture industry.
      PubDate: 2018-10-01
      DOI: 10.1007/s10499-018-0278-7
      Issue No: Vol. 26, No. 5 (2018)
       
  • J. Treasurer (editor): Cleaner fish biology and aquaculture applications
    • Authors: Malcolm Jobling
      Pages: 1207 - 1209
      PubDate: 2018-10-01
      DOI: 10.1007/s10499-018-0279-6
      Issue No: Vol. 26, No. 5 (2018)
       
  • Impact of food type on growth, survival and reproduction of the cyclopoid
           copepod Cyclopina kasignete as a potential live food in aquaculture
    • Authors: Nadiah W. Rasdi; Jian G. Qin
      Pages: 1281 - 1295
      Abstract: This study compared the efficacy of different dietary algae on the growth and reproduction of the cyclopoid copepod Cyclopina kasignete, a potential live food species for fish larvae in aquaculture. The experimental diets for the copepod consisted of three monoalgal diets (Nannochloropsis oculata, Tisochrysis lutea and dry Melosira sp.) and two mixed algae diets (T. lutea + N. oculata, T. lutea + dry Melosira sp.). The experiment was carried out for 30 days, and the population growth, survival and reproductive performance (generation time, hatching rate, life spawning times, daily offspring production, eggs per sac, lifespan and sex ratio) were used to assess the responses of C. kasignete to different food types. Population growth, survival and reproductive capacities of C. kasignete were significantly affected by the mono and binary species of algal diets. The results showed that copepods exhibited superior growth, survival and productivity when fed on fresh T. lutea, dry Melosira sp. and a mixture of both species compared to other dietary treatments. Copepods produced comparable growth, survival and productivity when fed on diatoms (dry Melosira sp.) as a single or in combination with other algae. This study indicates that cyclopoid copepod C. kasignete grow fast and have the potential to serve as a live food for aquaculture. The algae T. lutea, dry Melosira sp. and their combination are appropriate food to sustain the growth and reproduction of this copepods in mass culture as a potential live food in fish hatchery.
      PubDate: 2018-10-01
      DOI: 10.1007/s10499-018-0283-x
      Issue No: Vol. 26, No. 5 (2018)
       
  • Effects of autochthonous probiotics, isolated from Tor grypus (Karaman,
           1971) intestine and Lactobacillus casei (PTCC 1608) on expression of
           immune-related genes
    • Authors: Takavar Mohammadian; Mojtaba Alishahi; Mohammad Reza Tabandeh; Abdolhossein Jangaran Nejad; Esmaeil Karami; Mojtaba Zarea
      Abstract: In this study, Lactobacillus plantarum subsp. plantarum and L. delbrukei subsp. bulgaricus were used as autochthonous food supplementation for gene expression and hematological investigation. Then, 480 fish (BW, 45 ± 10 g) were randomly divided into four groups (three replications) and were fed with diet containing 5 × 107 CFU g−1 of each isolated LAB and L. casei (PTCC 1608) and a control diet with no bacteria. Feeding trail was conducted for 60 days, following a change to the non-supplemented feed until 15 days (day 75). Samples from blood and head kidney at days 0, 60, and 75 were taken. The in vitro examination showed that L. plantarum had high antimicrobial activity against the Aeromonas hydrophila and Yesinia ruckeri. Results of this study showed that feeding of T. grypus with L. plantarum supplementation diet led to increase Hb and WBC, and induce TNF-α, IL-8, and IL-1β expressions in the head kidney rather than control. Results suggested that L. plantarum can be applied as a dietary supplement to enhance the immunity system of T. grypus.
      PubDate: 2018-11-17
      DOI: 10.1007/s10499-018-0320-9
       
  • A first study on the effect of dietary soya levels and crystalline
           isoflavones on growth, gonad development and gonad histology of farmed
           abalone, Haliotis midae
    • Authors: Yu Wu; Horst Kaiser; Clifford L. W. Jones
      Abstract: Soya in formulated diets for abalone, Haliotis midae, can improve somatic growth. However, it may also influence gonad development, thereby limiting the potential benefit of including soya in feed. This is the first study to determine both the effect of dietary soya level on growth and reproduction of abalone and the role of crystalline isoflavones (ISO) at concentrations occurring in soya diets. Abalone (40–50 g individual−1) were fed seven isonitrogenous and isoenergetic diets. Three diets contained 5, 10 or 19% soya, while an additional three diets included graded levels of ISO. A fishmeal (FM)-only diet was included. The inclusion of ISO did not influence gonad development or abalone growth while soya inclusion level had an effect on both reproductive development and abalone growth. After 180 days, male abalone fed FM with soya inclusion had the highest whole body mass (69.0 ± 2.48 g abalone−1), meat mass index (60.5 ± 1.88%), visceral mass index (13.0 ± 0.27%) and gonad bulk index (42.7 ± 9.82 g abalone−1). Weight loss was observed in all treatments probably due to spawning events. The frequency distribution of the predominant oocyte stage, stage 7 was influenced by soya concentration. The increase in the frequency of stage 7 oocytes in abalone fed FM with soya did not reduce the number of oocytes (45.0 ± 3.01 oocytes mm−2), while the number of oocytes (o) in abalone fed the FM-only diets decreased with increasing abundance of stage 7 oocytes, possibly due to an increase in oocyte size.
      PubDate: 2018-11-15
      DOI: 10.1007/s10499-018-0315-6
       
  • Betanodavirus-associated mortality in Asian seabass ( Lates calcarifer ,
           Bloch) cultured in indoor tanks and sea cages
    • Authors: S. R. Krupesha Sharma; M. A. Pradeep; P. N. Dube; T. V. Arun Kumar; Raj Kumar; T. Raja Swaminathan
      Abstract: Sea cage farming of Asian seabass is a prospective aquaculture activity in India. Reports from India on betanodavirus-induced mortality in marine fish culture are not many. An outbreak of viral encephalopathy and retinopathy associated with red-spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus in Asian seabass juveniles reared in indoor tanks and sea cages is described in the present paper. Mortality was less severe in cages when compared to tanks. Sequence analysis of the coat protein gene indicated that the virus belonged to RGNNV genotype but found to be distinct from other reported RGNNV isolates. Virus was able to induce cytopathic effects in fish cell lines. Light microscopic features of the disease are also described.
      PubDate: 2018-11-14
      DOI: 10.1007/s10499-018-0322-7
       
  • A review on the impact of seaweed polysaccharide on the growth of
           probiotic bacteria and its application in aquaculture
    • Authors: S. Vidhya Hindu; Natarajan Chandrasekaran; Amitava Mukherjee; John Thomas
      Abstract: The purpose of this review is to elucidate the probiotic and polysaccharide of seaweeds as a dietary supplement for promoting growth and disease resistance in aquatic animals. Because of numerous benefits, the role of dietary supplementation of seaweed polysaccharide and probiotics in promoting growth and interest in commercially important aquatic animals like prawn and fish has generated considerable interest in recent times. Control of bacterial and viral disease and enhancing the immunity of aquatic animals have become urgent needs in aquaculture production. In order to improve the production, several alternative strategies have been raised using probiotics, seaweed polysaccharide (prebiotic), and plant products as biological control agents. Furthermore, the use of natural substances in feed of aquatic animals increases consumer confidence in farmed aquatic animals. Modulation of the intestinal microflora through diet can either be accomplished by means of oral administration of prebiotic compounds or probiotic microorganisms. In some studies for growth enhancement and improvement of immunological parameters, a combination of probiotics and plant products has been reported. The present review suggests the use seaweed polysaccharide and probiotics in combination as dietary supplements due to its beneficial effects to improve the production of healthy aquatic animals.
      PubDate: 2018-11-10
      DOI: 10.1007/s10499-018-0318-3
       
  • Transcriptional response of immune-related genes in Litopenaeus vannamei
           post-larvae cultured in recirculating aquaculture systems with and without
           biofloc
    • Authors: Jorge Soto-Alcalá; Píndaro Álvarez-Ruiz; J. M. Audelo-Naranjo; H. M. Esparza-Leal; I. E. Luis-Villaseñor; J. A. Estrada-Godínez; A. Luna-González; C. Gámez-Jiménez; G. Diarte-Plata
      Abstract: In the present study, quantitative real-time RT-PCR was used to monitor the transcriptional responses of seven key genes related to some innate immune pathways in shrimp postlarvae after being placed in a recirculating aquaculture system (RAS) with or without biofloc (BF or no-BF). Each system consisted of the main tank with 400 L of seawater and six glass aquariums (50 L each). Besides, the nucleotide sequences of myosin light chain (LvMyo) from Litopenaeus vannamei related to the phagocytosis pathway were described. The sequence analysis indicated that LvMyo is a conserved protein among crustaceans and is present in other arthropods. The transcriptional response to the treatments showed several expression patterns. The prophenoloxidase gene was up-regulated in both systems (P < 0.05) and was higher in BF than in no-BF (P < 0.01). Phagocytosis-related genes depicted differential expressions. LvMyo, Ras-associated binding 6, and Ras-related nuclear protein expressions were higher in BF than in no-BF (P < 0.05). Regarding antioxidant genes, glutathione peroxidase was up-regulated only in BF (P < 0.05). Superoxide dismutase expression was lower in BF at 12 h (P < 0.05), but higher at 24 h (P < 0.05). These findings suggest that biofloc modulates the transcription of genes related to the immune response in shrimp as an early response or at the mid-term. Besides, the biological filter in a RAS without biofloc seems to be able to maintain a bacterial population that promotes a lower but similar response to that induced in the biofloc system.
      PubDate: 2018-11-09
      DOI: 10.1007/s10499-018-0317-4
       
  • A microplate incubation method for assessing egg quality of the barfin
           flounder: effects of well size and rearing medium on larval viability
    • Authors: Tatsuya Unuma; Takashi Ichikawa; Sayumi Sawaguchi; Natsuki Hasegawa
      Abstract: In microplate incubation, fish eggs and larvae are individually reared in small wells. This technique is useful for assessing hatchability, larval survival, and larval abnormality. Nevertheless, the optimal well size (water volume) and rearing media required to ensure full larval viability vary among species. In this study, we examined the effects of well size and rearing medium on hatchability and larval viability of the barfin flounder Verasper moseri and attempted to optimize the incubation conditions for this species. Fertilized eggs were individually stocked in 24-, 48-, and 96-well plates filled with 2, 1, and 0.25 mL seawater containing antibiotics (penicillin G potassium and streptomycin sulfate) and bovine serum albumin (BSA), both of which are required in the rearing media for other species. The eggs were incubated without replacing the medium. Hatch timing and rate were similar in all plate types. Completion of yolk resorption was delayed only in the 96-well plates. In the 24- and 48-well plates, the larvae survived more than 2 weeks after complete yolk resorption until they were starved to death. When the antibiotics were removed from the media, larval mortality increased. In contrast, removal of BSA from the media did not increase yolk sac stage larval mortality for barfin flounder unlike that reported for other species. These results indicate that the use of 24- and 48-well microplates filled with seawater containing antibiotics is optimal for barfin flounder larval viability beyond the completion of yolk resorption.
      PubDate: 2018-11-08
      DOI: 10.1007/s10499-018-0316-5
       
  • Effect of Bacillus subtilis as a probiotic on protein, lipid content, and
           trypsin and chymotrypsin enzymes in rainbow trout biometry ( Oncorhynchus
           mykiss )
    • Authors: Fereshte Sahraei; Hamed Ahari; Shapoor Kakoolaki
      Abstract: This study investigated the effect of Bacillus subtilis IS02 as a probiotic bacterium in four different treatment conditions, namely T1 (no probiotic), T2 (106), T3 (107), and T4 (108) cfu/g of B. subtilis IS02 in the diet of rainbow trout for 30 days, on the protein content, lipid content, trypsin and chymotrypsin enzyme activity, and the bioassay. The protein content and lipid content were measured using the Kjeldahl and Soxhlet methods, respectively.T4 had the highest amount of measured protein (25.30%) while T1 had the lowest amount (15.90). The T4 treatment had a significant effect (P value = 0.001) on improving the protein content as well as the trypsin and chymotrypsin enzyme activity. In contrast, there was no significant difference in terms of the weight gain and specific growth rate between the treatments. Additionally, the results showed that the T3 treatment had a significant effect on increasing the protein content of the carcass, although the effect was not significant in relation to the lipid content. B. subtilis IS02 in a concentration range of 107–108 cfu/g could be employed to improve the protein productive value, trypsin and chymotrypsin enzyme activity, and protein content of the carcass.
      PubDate: 2018-10-27
      DOI: 10.1007/s10499-018-0313-8
       
  • Environmental stress tolerance and immune response for the small abalone
           hybrids
    • Authors: Weiwei You; Bo Wang; Xuan Luo; Caihuan Ke
      Abstract: Recently, mass mortality affected the cultured small abalone, Haliotis diversicolor diversicolor, which was the dominant cultured abalone species in southern China. Prior studies revealed that survivorship varied significantly between different stocks and crosses. However, the immunological basis for differences in susceptibility has not been well understood to date. Herein, low temperature, air exposure tolerance tests, and pull-off force measurement were assessed in the three groups (Japan, Taiwan, and their Hybrid stock). The results showed that the critical thermal minimum (CTMin) at 50% was 15.6 °C for the Taiwan stock, 12.1 °C for the Japan stock, and 13.2 °C for the Hybrid stock. Upon air exposure challenge, 100% abalones from the Taiwan group died after 8 h at 24 °C, while the survival rate in the Japan and Hybrid groups were 37.8% and 29.4%, respectively. The detachment stress for the Japan group was 42.3 kPa, which was 2.78-fold and 1.43-fold higher compared to the Taiwan and Hybrid groups, respectively. Variation in susceptibility to disease may be based on the effectiveness of the innate immune responses. Therefore, total hemocyte count, phagocytosis, respiratory burst, superoxide dismutase activity, acid phosphatase activity, alkaline phosphatase activity, and myeloperoxidase activity were determined for the healthy abalones in each group. Positive mid-parent heterosis on immunological parameters was consistent with the prior knowledge on the positive mid-parent heterosis for survival rate, which indicated the improvement on immune reaction and disease resistance through hybridization methods. The current study will be useful in efficient design of breeding programs for the development of sustainable abalone aquaculture.
      PubDate: 2018-10-26
      DOI: 10.1007/s10499-018-0310-y
       
  • Effects of different incubation and start-feeding temperature regimes on
           growth, survival, and histomorphology of cod larvae
    • Authors: Inger-Britt Falk-Petersen; Stefano Peruzzi; Hege Lysne; Hilde Toften; Helge Tveiten; Bjørn Steinar Sæther; Velmurugu Puvanendran
      Abstract: Mean lengths of newly hatched cod larvae from egg incubation at 4 °C (Low-L) and 9.5 °C (High-H) were similar, and only minor differences were observed in larval histomorphology. However, growth performance of larvae reared at 4 °C (T1-LL) and 9.5 °C (T2-LH) from the 4 °C egg incubation and 4 °C (T3-HL) and 9.5 °C (T4-HH) from the 9.5 °C incubation group were different during start-feeding and metamorphosis. Incubation and larval rearing temperature affected developmental rate and survival. T1-LL and T2-LH larvae were larger than T4-HH larvae at sampling stage 4 (early larvae), but the differences disappeared thereafter. Larvae from T3-HL did not survive beyond stage 8 (late larvae/start metamorphosis), and survival of T1-LL larvae at the end of the experiment was very low. Larvae from T2-LH were significantly larger than larvae from T4-HH at stage 12 (end metamorphosis). Comparative studies of the histomorphology of vital organs did not reveal temperature-related differences between sampled larvae/early juveniles. Characteristic traits in the histomorphology of cod larvae at the selected developmental stages are presented. Our results show that egg incubation and subsequent larval rearing temperature affected the growth performance and survival but did not have any significant effects on the organ development and histomorphology.
      PubDate: 2018-10-25
      DOI: 10.1007/s10499-018-0314-7
       
  • Public attitudes towards marine aquaculture in Canada: insights from the
           Pacific and Atlantic coasts
    • Authors: Mark Flaherty; Gregor Reid; Thierry Chopin; Erin Latham
      Abstract: With 25% of the world’s coastline, Canada is endowed with enormous potential for marine aquaculture. Its development on both the Pacific and Atlantic coasts, however, has come under increasingly intense public scrutiny and generated heated debate over many issues including First Nations territorial rights, impacts on wild fisheries, and environmental impacts. Provincial governments, industry alliances, scientists and ENGOs (environmental non-governmental organizations) have become increasingly embroiled in a communication tug-of-war to win the hearts and minds of the public. To date, however, there has been very limited community-level research into the public’s awareness of aquaculture, the information sources viewed as being most credible, and the issues that resonate the most. This paper reports the results of a study undertaken in small coastal communities on Canada’s Pacific (west) and Atlantic (east) coasts that investigated awareness of aquaculture, and the issues that people associate with its development. While many studies have focused exclusively on attitudes related to either finfish or shellfish farming, this study explores community perceptions related to salmon, shellfish and seaweed farming. Significant differences exist between the Atlantic and Pacific coast respondents in terms of their familiarity with and evaluation of different farming systems, the information sources that they rely upon, and their perceptions of the reliability of different information sources.
      PubDate: 2018-10-25
      DOI: 10.1007/s10499-018-0312-9
       
  • Vibrio harveyi protease deletion mutant as a live attenuated vaccine
           candidate against vibriosis and transcriptome profiling following
           vaccination for Epinephelus fuscoguttatus
    • Authors: Aslizah Mohd-Aris; Mohd-Zamri Saad; Hassan Mohd Daud; Mohd Termizi Yusof; Md Yasin Ina-Salwany
      Abstract: Grouper aquaculture industries have a high risk of being inflicted by bacterial diseases such as vibriosis. Various types of vaccines for vibriosis have been studied throughout the years, yet the potential of live attenuated vaccines remains unsubstantial. Correspondingly, this study attempts to develop a Vibrio harveyi protease deletion mutant into a live attenuated vaccine candidate against vibriosis for Epinephelus fuscoguttatus. Site-directed mutagenesis (SDM) and allelic exchange replacement techniques are employed to synthesize genetically attenuated V. harveyi strain MVh-vhs. The evaluation on safety levels showed that MVh-vhs strain is safe when tested on E. fuscoguttatus. A 100% survival rate with no sign of vibriosis is indicated in fish challenged with the attenuated strain. In contrast, fish challenged with the parental strain showed obvious clinical signs of vibriosis. The median lethal dosage (LD50) of fish challenged with the parental strain is found at 106 CFU/fish. A single dose IP administration of the attenuated strain at 105 CFU/fish following a bacterial challenge at dose 108 CFU/fish is done 4 weeks post vaccination. The vaccinated fish show 52% relative percentage survival (RPS). The transcriptomic profiling following vaccination evoked the regulation of autophagosome pathway and the coagulation and complement cascade pathways as well as antigen processing and presentation pathways. As a conclusion, the V. harveyi attenuated strain MVh-vhs has significant potential to be applied as a live vaccine candidate against vibriosis for E. fuscoguttatus.
      PubDate: 2018-10-25
      DOI: 10.1007/s10499-018-0311-x
       
  • Biodeposits from Mytilus edulis : a potentially high-quality food source
           for the polychaete, Hediste diversicolor
    • Authors: Per Bergström; Niklas Hällmark; Karl-Johan Larsson; Mats Lindegarth
      Abstract: Previous studies have shown clearly that the deposit feeding polychaete, Hediste diversicolor, can promote oxygenation of sediments exposed to excess loads of mussel faeces. In this experimental study, we explicitly test their utility as food for H. diversicolor to survive and grow on. Furthermore, in order to understand the consequences of experimental manipulations, we also evaluated effects on chemical fluxes in and out of the sediment. The results show strong differences in growth but no difference in short-term survival. Fed only on mussel faeces, the polychaetes grew on average 17% in wet weight after a period of 10 days, compared to 3% when given equivalent amounts of organic matter from the natural sediments. Addition of faeces to natural sediments resulted in 19–20% growth, thus suggesting an approximate additive effect of the two food sources. Chemical analyses showed that, oxygen consumption increased with load of organic material irrespective of origin, faecal material caused higher fluxes of ammonia compared to natural organic material, but neither oxygen consumption nor nutrient fluxes were affected by the ashing of sediments. In contrast, fluxes of silicate increased as a consequence of ashing but were not affected by the addition of mussel faeces. Thus, despite risks of experimental artefacts due to ashing of sediments, the results show that oxygen and nutrient dynamics responded to manipulations of organic material and not to the potential modification of sediment structure. Therefore, the observed effects on growth of H. diversicolor can be safely interpreted as caused by differences in amount and quality of organic material. Mussel faeces is a high-quality food source for this species of polychaete and, in combination with ample evidence from previous studies that bioturbation, we conclude that H. diversicolor is a suitable candidate in further efforts to develop technical solutions based on bioturbation for mitigation of adverse effects on benthic environments in connection with mussel-farming.
      PubDate: 2018-10-25
      DOI: 10.1007/s10499-018-0309-4
       
  • The effect of dipotassium EDTA and lithium heparin on hematologic values
           of farmed brown trout Salmo trutta (L.) spawners
    • Authors: Mateusz Ciepliński; Mariusz Kasprzak; Monika Grandtke; Aleksandra Steliga; Piotr Kamiński; Leszek Jerzak
      Abstract: Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is routinely performed in higher vertebrates, for which reference values are well established, but fish hematology still needs further research. Many intrinsic and environmental factors have profound impact on fish hematological values, making determination of reference values difficult. Additionally, fish blood usually requires the addition of an anticoagulant agent, because of short clotting times. The choice of anticoagulant is vital for obtaining reliable blood test values. In the present study, the impact of two common anticoagulants, K2EDTA (1.8 mg/ml) and lithium heparin (18 I.U./ml), on hematological values of farmed brown trout Salmo trutta spawners during the spawning season was investigated. Results of basic hematological analysis, such as packed cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and white blood cell count (WBC), were compared between these two compounds. Statistically significant differences were observed in PCV, MCV, and MCHC, whereas HGB, RBC, MCH, and WBC showed no such differences. These results suggest that lithium heparin gives more reliable results, because red blood cells in K2EDTA-treated samples have a tendency to swell. It is worth noting that ethylenediaminetetraacetic acid salt did not induce sample hemolysis in the present study.
      PubDate: 2018-10-24
      DOI: 10.1007/s10499-018-0308-5
       
  • In vitro evaluation of the probiotic candidates isolated from the gut of
           Clarias gariepinus with special reference to the in vivo assessment of
           live and heat-inactivated Leuconostoc mesenteroides and Edwardsiella sp.
    • Authors: Khaled M. Selim; Hassanin M. El-Sayed; M. A. El-Hady; Rasha M. Reda
      Abstract: Fish gut microbiota contains many potential probiotics that can be used in aquaculture. The aim of this study was to isolate beneficial probiotic bacteria from the gut of African catfish, Clarias gariepinus, and to evaluate the most suitable in vivo probiotic candidates. Eighty-nine gut bacterial isolates were screened for in vitro antibacterial activities against five common isolated fish pathogens, Aeromonas sobria, A. hydrophila, Pseudomonas aeruginosa, P. putida, and Staphylococcus aureus. Results found that nine out of 89 isolates had antibacterial activities with at least one pathogen. Two out of nine isolates demonstrated pathogenic effects following intraperitoneal injection. The remaining non-pathogenic seven strains were able to survive in high bile concentration (10%) and at different acidic pH values. Three out of seven strains were sensitive to all selected antibiotics. Four out of seven strains had amylase and protease activities. Based on 16S rRNA gene analysis, the isolated strains were identified as follows: Bacillus subtilis (6HN), B. amyloliquefaciens (7HN), B. cereus (29HN), Leuconostoc mesenteroides (13HN), Edwardsiella sp. (34HN), and Citrobacter freundii (24HN and 31HN). Comparative in vivo study of dietary supplementations of heat-inactivated and live L. mesenteroides (13HN) and Edwardsiella sp. (34HN) showed better improvements in growth, immunity, and the expression of myostatin (MSTN) and pituitary adenylate cyclase-activating polypeptide (PACAP) in the spleen of C. gariepinus of heat-inactivated probiotics than live one.
      PubDate: 2018-10-22
      DOI: 10.1007/s10499-018-0297-4
       
  • Effect of dietary administration of kappa carrageenan extracted from
           Hypnea musciformis on innate immune response, growth, and survival of Nile
           tilapia ( Oreochromis niloticus )
    • Authors: L. Villamil; S. Infante Villamil; G. Rozo; J. Rojas
      Abstract: Immune stimulants are an alternative to antibiotic use and contribute to disease prevention in aquaculture. The effect of dietary administration of kappa carrageenan (Kc), extracted from the red algae Hypnea musciformis, in Nile tilapia (Oreochromis niloticus) was assessed by monitoring weight gain after a 15-day feeding trial. Immunostimulation was inferred by the relative expression of transferrin, interleukin 1β (IL-1β), and growth hormone (GH) in the spleen after 24 h and 15 days of daily administration. The toxic activity of Kc was evaluated in brine shrimp (Artemia salina) nauplii. No significant toxic effects of Kc were observed in A. salina at any dose studied. A positive tendency in growth rate and fish survival values was observed when Kc was administered. Correspondingly, GH, transferrin and IL-1 β levels at day 15 post-treatment were higher in the spleens of fish fed with Kc at 5 g kg−1 relative to non-Kc-treated control fish. Feeding Kc extract from H. musciformis to the fish improved nonspecific immunity parameters and increased survival and growth, but further research, including longer-termed studies, should be conducted before recommendation of Kc supplementation in tilapia diets at commercial scale.
      PubDate: 2018-10-03
      DOI: 10.1007/s10499-018-0306-7
       
  • Immunoserology of European seabass ( Dicentrarchus labrax ) and white
           grouper ( Epinephelus aeneus ) as a non-lethal diagnostic tool for viral
           nervous necrosis
    • Authors: Koby Tarrab; Shay Ravid-Peretz; Michal Ucko
      Abstract: Viral nervous necrosis (VNN) is a lethal fish disease that has spread worldwide over the last two decades, causing severe losses in aquaculture. Diagnosis of the infection is generally made by sampling brain tissue, which involves sacrificing often valuable fish. Aiming at developing a non-lethal diagnostic method, the immune responses to an experimental nervous necrosis virus (NNV) infection in sea bass Dicentrarchus labrax and white grouper Epinephelus aeneus, two species most susceptible to the disease, were studied. RT-qPCR revealed presence of NNV in the fish brain within 24 h post-infection, the virus titer remaining high up to 30–35 days post-infection. In D. labrax blood, the virus was detectable within the first 5 days, after which its presence declined rapidly. Mx gene expression correlated to the virus presence in the blood and brain. An indirect ELISA was developed that quantified anti-NNV IgM in the fish blood. In D. labrax, anti-NNV IgM titer increased significantly within 5 days post-infection, and presence of specific IgM was detectable for 180 days. A sandwich ELISA was developed for E. aeneus. In this latter species, anti-NNV IgM titer increased significantly within the first 12 days and was detectable for 208 further days. The sandwich ELISA can be used as a diagnostic tool for detecting NNV exposure in all fish species for which specific antibodies against their IgMs are not yet commercially available. Our immunoserological method can reliably be used for diagnosis of VNN infection and does not require sacrificing the fish.
      PubDate: 2018-10-03
      DOI: 10.1007/s10499-018-0307-6
       
 
 
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