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  Subjects -> CHEMISTRY (Total: 814 journals)
    - ANALYTICAL CHEMISTRY (49 journals)
    - CHEMISTRY (566 journals)
    - CRYSTALLOGRAPHY (22 journals)
    - ELECTROCHEMISTRY (25 journals)
    - INORGANIC CHEMISTRY (41 journals)
    - ORGANIC CHEMISTRY (46 journals)
    - PHYSICAL CHEMISTRY (65 journals)

INORGANIC CHEMISTRY (41 journals)

Acta Polymerica     Hybrid Journal   (Followers: 6)
Additives for Polymers     Full-text available via subscription   (Followers: 21)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 4)
Advances in Polymer Technology     Hybrid Journal   (Followers: 11)
Annual Reports Section A (Inorganic Chemistry)     Full-text available via subscription   (Followers: 2)
Bioinorganic Chemistry and Applications     Open Access   (Followers: 4)
Comments on Inorganic Chemistry: A Journal of Critical Discussion of the Current Literature     Hybrid Journal  
Current Methods in Inorganic Chemistry     Full-text available via subscription  
European Journal of Inorganic Chemistry     Hybrid Journal   (Followers: 6)
European Polymer Journal     Hybrid Journal   (Followers: 41)
Heterocyclic Communications     Full-text available via subscription   (Followers: 2)
High Performance Polymers     Hybrid Journal  
Indian Journal of Chemistry - Section A     Open Access   (Followers: 5)
Inorganic Chemistry     Full-text available via subscription   (Followers: 19)
Inorganic Chemistry Communications     Hybrid Journal   (Followers: 7)
Inorganic Chemistry Frontiers     Full-text available via subscription  
Inorganic Materials     Hybrid Journal   (Followers: 2)
Inorganic Materials: Applied Research     Hybrid Journal   (Followers: 1)
Inorganica Chimica Acta     Hybrid Journal   (Followers: 4)
Inorganics     Open Access  
International Journal of Inorganic Chemistry     Open Access   (Followers: 1)
JBIC Journal of Biological Inorganic Chemistry     Hybrid Journal   (Followers: 2)
Journal of Inorganic and Organometallic Polymers and Materials     Partially Free   (Followers: 6)
Journal of Inorganic Biochemistry     Hybrid Journal   (Followers: 2)
Journal of Inorganic Chemistry     Open Access  
Journal of Polymer Engineering     Full-text available via subscription   (Followers: 8)
Journal of Polymers and the Environment     Hybrid Journal   (Followers: 3)
Journal of Separation Science     Hybrid Journal   (Followers: 7)
Metallodrugs     Open Access  
Open Journal of Inorganic Chemistry     Open Access   (Followers: 1)
Plasmas and Polymers     Hybrid Journal  
Polymer Bulletin     Hybrid Journal   (Followers: 7)
Polymer Composites     Hybrid Journal   (Followers: 8)
Polyoxometalate Chemistry     Open Access  
Reviews in Inorganic Chemistry     Full-text available via subscription  
Russian Journal of Inorganic Chemistry     Hybrid Journal  
Studies in Inorganic Chemistry     Full-text available via subscription  
Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry     Hybrid Journal   (Followers: 3)
Topics in Inorganic and General Chemistry     Full-text available via subscription  
Zeitschrift für anorganische und allgemeine Chemie     Hybrid Journal   (Followers: 1)
Zeitschrift für Kristallographie - New Crystal Structures     Open Access  
Journal Cover   Journal of Inorganic Biochemistry
  [SJR: 0.807]   [H-I: 79]   [4 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0162-0134
   Published by Elsevier Homepage  [2589 journals]
  • Hydrogen generation: Aromatic dithiolate-bridged metal carbonyl complexes
           as hydrogenase catalytic site models
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Indresh Kumar Pandey , Mookan Natarajan , Sandeep Kaur-Ghumaan
      The design, syntheses and characteristics of metal carbonyl complexes with aromatic dithiolate linkers reported as bioinspired hydrogenase catalytic site models are described and reviewed. Among these the complexes capable of hydrogen generation have been discussed in detail. Comparisons have been made with carbonyl complexes having alkyl dithiolates as linkers between metal centers.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Cu(II)–dipeptide complexes of 2-(4′-thiazolyl)benzimidazole:
           Synthesis, DNA oxidative damage, antioxidant and in vitro antitumor
           activity
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Xia-Bing Fu , Jia-Jia Zhang , Dan-Dan Liu , Qian Gan , Hong-Wei Gao , Zong-Wan Mao , Xue-Yi Le
      Two new Cu(II)–dipeptide complexes of 2-(4′-thiazolyl)benzimidazole, [Cu(Gly-Gly)(TBZ)(Cl)]·4H2O (1) and [Cu(Gly- l -Leu)(TBZ)(Cl)]·H2O (2) (Gly-Gly=glycyl-glycine anion, Gly- l -Leu=glycyl- l -leucine anion and TBZ=2-(4′-thiazolyl)benzimidazole) have been synthesized and characterized by elemental analyses, molar conductance measurements and spectroscopy methods (IR, UV–visible, electrospray ionization mass spectra (ESI-MS) and EPR). The DNA binding and cleavage properties of the complexes monitored by multi-spectroscopic techniques (UV absorption, fluorescence and circular dichroism), viscosity determination and agarose gel electrophoresis indicated that the complexes bound to calf thymus (CT)-DNA via a partial intercalative mode with considerable intrinsic binding constants (K b =1.64×105 M−1 for 1 and 2.59×105 M−1 for 2), and cleaved pBR322 DNA efficiently in the mediation of ascorbic acid (AA), probably via an oxidative damage mechanism induced by OH. The antioxidant activities of the complexes have been evaluated by means of modified nitroblue tetrazolium (NBT) photoreduction and cellular antioxidant activity (CAA) assays using HepG2 cells as a model, and it was found that IC50 values of 1 and 2 for dismutation of O2 − were 0.172 and 0.247μM, respectively, and the CAA50 values were 10.57 and 10.74μM. In addition, the complexes were subjected to in vitro cytotoxicity against three human carcinoma cell lines (HeLa, A549 and HepG2), which revealed that the complexes exhibited effective cytotoxicity (IC50 values varying from 33.17 to 100μM) and selective inhibition toward HeLa cell lines. These findings indicate that the complexes have the potential to act as effective metallopeptide chemotherapeutic agents.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Evaluation of the cell cytotoxicity and DNA/BSA binding and cleavage
           activity of some dioxidovanadium(V) complexes containing aroylhydrazones
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Subhashree P. Dash , Alok K. Panda , Sagarika Pasayat , Sudarshana Majumder , Ashis Biswas , Werner Kaminsky , Subhadip Mukhopadhyay , Sujit K. Bhutia , Rupam Dinda
      Three dioxidovanadium(V) complexes [VO2L1–3] (1–3) [HL1 =1-napthoyl hydrazone of 2-acetyl pyridine, HL2 =2-furoyl hydrazone of 2-acetyl pyridine and H2L3 =isonicotinoyl hydrazone of 2-hydroxy benzaldehyde] have been reported. All the complexes were characterized by various spectroscopy (IR, UV-visible and NMR) and the molecular structures of 1 and 2 were characterized by single crystal X-ray diffraction technique. Structural report established five-coordinate geometries, distorted toward square pyramidal for each of 1 and 2, based on a tridentate -O,N,N coordinating anion and two oxido–O atoms. The experimental results show that the complexes interact with calf–thymus DNA (CT-DNA) possibly by a groove binding mode, with binding constants of ~105 M−1. All complexes show good photo-induced cleavage of pUC19 supercoiled plasmid DNA with complex 1 showing the highest photo-induced DNA cleavage activity of ~68%. 1–3 also exhibit moderate binding affinity in the range of 103–104 M−1 towards bovine serum albumin (BSA), while all the complexes show good photo-induced BSA cleavage activity. Moreover the antiproliferative activity of all these complexes was studied, which reveal all compounds are significantly cytotoxic towards the HeLa cell line.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Editorial Board
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144




      PubDate: 2015-02-28T12:16:33Z
       
  • Contents
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144




      PubDate: 2015-02-28T12:16:33Z
       
  • Structural biology of the lanthanides—mining rare earths in the
           Protein Data Bank
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Kristina Djinovic-Carugo , Oliviero Carugo
      With its about 100,000 three-dimensional structures, the Protein Data Bank is a copious source of information: it contains also some hundreds of structures of macromolecules complexed with lanthanide cations, which are examined here. These cations, which are found in a wide variety of protein types, were introduced to determine the structures, by exploiting their anomalous dispersion (in crystallographic studies, where they are also used as crystallization additives) or the paramagnetic pseudocontact shifts (in NMR analyses). The coordination numbers in the first coordination sphere are very variable, though they tend to be close to those that are observed in small molecules or in water solution. The coordination polyhedra are also quite variable as it can be expected for large cations. Interestingly, lanthanide cations are frequently observed in packing bridges between symmetry equivalent molecules in crystals, where they tend to form polynuclear complexes, with up to seven cations bridged by water/hydroxide ligands.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Photoinduced DNA damage and cytotoxicity by a triphenylamine-modified
           platinum-diimine complex
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Zhigang Zhang , Ruihui Dai , Jiajia Ma , Shuying Wang , Xuehong Wei , Hongfei Wang
      Many planar photosensitizers tend to self-aggregate via van der Waals interactions between π-conjugated systems. The self-aggregation of the photosensitizer may reduce the efficiency of the photosensitizer to generate singlet oxygen, thereby diminishing its photodynamic activity. Efforts have been made to improve the photodynamic activity of bis-(o-diiminobenzosemiquinonato)platinum(II) which has planar geometry by the introduction of the sterically hindered triphenylamine moiety into the ligand. Herein we report the photoinduced DNA damage and cytotoxicity by a triphenylamine-modified platinum-diimine complex in red light studied by fluorescence spectra, agarose gel assay and cell viability assay. The results suggest that the triphenylamine-modified platinum-diimine complex has better capability to generate singlet oxygen than bis-(o-diiminobenzosemiquinonato)platinum(II), and it can induce DNA damage in red light, causing high photocytotoxicity in HepG-2 cells in vitro.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Effect of ancillary ligands on the interaction of ruthenium(II) complexes
           with the triplex RNA poly(U)·poly(A)*poly(U)
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Jia Li , Yanmei Sun , Lingjun Xie , Xiaojun He , Lifeng Tan
      Two new Ru(II) complexes with 1,8-naphthalimide group, [Ru(phen)2(pnip)]2+ (Ru1; phen=1,10-phenanthroline, pnip=2-[N-(p-phenyl)-1,8-napthalimide]imidazo[4',5'-f][1,10]phenanthroline) and [Ru(bpy)2(pnip)]2+ (Ru2; bpy=2,2′-bipyridine), have been synthesized and characterized. The interactions of Ru1 and Ru2 with the triplex RNA poly(U)•poly(A)*poly(U) (where • denotes the Watson–Crick base pairing and * denotes the Hoogsteen base pairing) were studied by various biophysical. Electronic spectra established that the binding affinity for Ru1 was greater than that for Ru2. Fluorescence and viscosity studies gave convincing evidence for a true intercalative binding of both complexes with the RNA triplex. UV melting studies confirmed that the two complexes could stabilize the triplex, whereas the effects of the two complexes on the stability of the Hoogsteen base-paired strand ploy(U) and the Watson–Crick base-paired duplex poly(U)•poly(A) of the triplex were different. In the case of Ru1, the increase of the thermal stability of the Hoogsteen base-paired strand was stronger than that of the Watson–Crick base-paired duplex. However, an opposite effect was observed in the case of Ru2. Circular dichroic studies suggested that the RNA triplex undergoes a conformational transition in the presence of Ru1, whereas the helicity of the RNA triplex still remains A-type in the presence of Ru2. The main results obtained here further advance our knowledge on the interaction of RNA triple-stranded structures with metal complexes, particularly ruthenium(II) complexes.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Insight on the interaction of an agmatinase-like protein with Mn2+
           activator ions
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Matías Quiñones , Jaime Cofre , José Benítez , David García , Nicol Romero , Arlette González , Nelson Carvajal , María García , Vasthi López , Gerhard Schenk , Elena Uribe
      Agmatinase is an enzyme that catalyzes the hydrolysis of agmatine, a compound that is associated with numerous functions in the brain of mammalian organisms such as neurotransmitter, anticonvulsant, antinociceptive, anxiolytic and antidepressant-like actions. To date the only characterized agmatinases with significant enzymatic activity were extracted from bacterial organisms. These agmatinases are closely related to another ureahydrolase, arginase; both have binuclear Mn2+ centers in their active sites. An agmatinase-like protein (ALP) from rat brain was identified that bears no sequence homology to known agmatinases (E. Uribe, M. Salas, S. Enriquez, M.S. Orellana, N. Carvajal, Arch. Biochem. Biophys. 461(2007) 146–150). Since all known ureahydrolases contain histidines in their binuclear Mn2+ site each of the five histidine residues in ALP was individually replaced by alanines to identify those that may be involved in metal ion binding. Reactivation assays and thermal stability measurements indicated that His206 is likely to interact with a Mn2+ bound to a high affinity site. In contrast, His65 and possibly His435 are important for binding of a second Mn2+ to a lower affinity site. Metal ion binding to that site is not only leading to an increase in reactivity but also enzyme stability. Thus, similar to bacterial agmatinases and some of the antibiotic-degrading, Zn2+-dependent metallo-β-lactamases ALP appears to be active in the mono and binuclear form, with binding of the second metal ion increasing both reactivity and stability.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Quercetin encapsulation in modified silica nanoparticles: potential use
           against Cu(II)-induced oxidative stress in neurodegeneration
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Christiane M. Nday , Eleftherios Halevas , Graham E. Jackson , Athanasios Salifoglou
      Neurodegenerative diseases entail deeply complex processes, intimately associated with progressive brain damage reflecting cellular demise. Biochemical reactivity linked to such processes in Alzheimer’s disease involves, among others, metal-induced oxidative stress contributing to neuronal cell death. Prominent among redox active metals inducing oxidative stress is Cu(II). Poised to develop molecular technology counteracting oxidative stress, efforts were launched to prepare bioactive hybrid nanoparticles, capable of working as host-carriers of potent antioxidants, such as the natural flavonoid quercetin. Employing synthetic protocols consistent with the assembly of silica nanoparticles, PEGylated and CTAB-modified materials were synthesized. Subsequent concentration-dependent loading of quercetin led to well-defined molecular carriers, the antioxidant efficiency of which was determined through drug release studies using UV-visible spectroscopy. The physicochemical characterization (elemental analysis, particle size, z-potential, FT-IR, thermogravimetric analysis, scanning electron microscopy) of the empty and loaded silica nanoparticles led to the formulation of optimized material linked to the delivery of the encapsulated antioxidant to primary rat hippocampal cultures under oxidative stress. Entrapment and drug release studies showed a) the competence of hybrid nanoparticles as far as the loading capacity in quercetin (concentration dependence), b) congruence with the physicochemical features determined, and c) the release profile of the nanoparticle load under oxidative stress in neuronal cultures. The bio-activity profile of quercetin nanoparticles in a neurodegenerative environment brought on by Cu(II) a) denotes the improved specificity of antioxidant reactivity counteracting oxidative stress, and b) sets the stage for the development of molecular protection and preventive medical nanotechnology of relevance to neurodegenerative Alzheimer’s disease.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Binding of trivalent chromium to serum transferrin is sufficiently rapid
           to be physiologically relevant
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Ge Deng , Kristi Wu , Alex A. Cruce , Michael K. Bowman , John B. Vincent
      Transferrin, the major iron transport protein in the blood, also transports trivalent chromium in vivo. Recent in vitro studies have, however, suggested that the binding of chromic ions to apotransferrin is too slow to be biologically relevant. Nevertheless, the in vitro studies have generally failed to adequately take physiological bicarbonate concentrations into account. In aqueous buffer (with ambient (bi)carbonate concentrations), the binding of chromium to transferrin is too slow to be physiologically relevant, taking days to reach equilibrium with the protein's associated conformational changes. However, in the presence of 25mM (bi)carbonate, the concentration in human blood, chromic ions bind rapidly and tightly to transferrin. Details of the kinetics of chromium binding to human serum transferrin and conalbumin (egg white transferrin) in the presence of bicarbonate and other major potential chromium ligands are described and are consistent with transferrin being the major chromic ion transporter from the blood to tissues.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Chelating ability and biological activity of hesperetin Schiff base
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Elzbieta Lodyga-Chruscinska , Marzena Symonowicz , Anna Sykula , Anna Bujacz , Eugenio Garribba , Magdalena Rowinska-Zyrek , Stanislaw Oldziej , Elzbieta Klewicka , Magdalena Janicka , Karolina Krolewska , Marcin Cieslak , Katarzyna Brodowska , Longin Chruscinski
      Hydrazone hesperetin Schiff base (HHSB) — N-[(±)-[5,7-dihydroxy-2-(3-hydroxy-4-methoxy-phenyl)chroman-4-ylidene]amino]benzamide has been synthesized and its crystal structure was determined. This compound was used for the formation of Cu(II) complexes in solid state and in solution which were characterized using different spectroscopic methods. The analyses of potentiometric titration curves revealed that monomeric and dimeric complexes of Cu(II) are formed above pH7. The ESI–MS (electrospray ionization–mass spectrometry) spectra confirmed their formation. The EPR and UV–visible spectra evidenced the involvement of oxygen and nitrogen atoms in Cu(II) coordination. Hydrazone hesperetin Schiff base can show keto-enol tautomerism and coordinate Cu(II) in the keto (O−, N, Oket) and in the enolate form (O−, N, O− enol). The semi-empirical molecular orbital method PM6 and DFT (density functional theory) calculations have revealed that the more stable form of the dimeric complex is that one in which the ligand is present in the enol form. The CuHHSB complex has shown high efficiency in the cleavage of plasmid DNA in aqueous solution, indicating its potential as chemical nuclease. Studies on DNA interactions, antimicrobial and cytotoxic activities have been undertaken to gain more information on the biological significance of HHSB and copper(II)–HHSB chelate species.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Structural and theoretical studies on rhodium and iridium complexes with
           5-nitrosopyrimidines. Effects on the proteolytic regulatory enzymes of the
           renin–angiotensin system in human tumoral brain cells
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Nuria A. Illán-Cabeza , Sonia B. Jiménez-Pulido , María J. Ramírez-Expósito , Antonio R. García-García , Tomás Peña-Ruiz , José M. Martínez-Martos , Miguel N. Moreno-Carretero
      The reactions of [RhCl(CO)(PPh3)2], [RhCl(CO)2]2 and [IrCl(CO)(PPh3)2] with different 5-nitrosopyrimidines afforded sixteen complexes which have been structurally characterized by elemental analysis, IR and NMR (1H and 13C) spectral methods and luminescence spectroscopy. The crystal and molecular structures of [RhIIICl(VIOH−1)2(PPh3)], [RhIIICl(DVIOH−1)2(PPh3)] and [RhII(DVIOH−1)2(PPh3)2] have been established from single crystal x-ray structure analyses. The three complexes are six-coordinated with both violurato ligands into an equatorial N5,O4-bidentate fashion, but with different mutually arrangements. Theoretical studies were driven on the molecular structure of [RhIIICl(VIOH−1)2(PPh3)] to assess the nature of the metal–ligand interaction as well as the foundations of the cis–trans (3L–2L) isomerism. An assortment of density functional (SOGGA11-X, B1LYP, B3LYP, B3LYP-D3 and wB97XD) has been used, all of them leading to a similar description of the target system. Thus, a topological analysis of the electronic density within AIM scheme and the study of the Mulliken charges yield a metal–ligand link of ionic character. Likewise, it has been proved that the cis–trans isomerism is mainly founded on that metal–ligand interaction with the relativistic effects playing a significant role. Although most of the compounds showed low direct toxicity against the human cell lines NB69 (neuroblastoma) and U373-MG (astroglioma), they differently modify in several ways the renin–angiotensin system (RAS)-regulating proteolytic regulatory enzymes aminopeptidase A (APA), aminopeptidase N (APN) and insulin-regulated aminopeptidase (IRAP). Therefore, these complexes could exert antitumor activity against both brain tumor types, acting through the paracrine regulating system mediated by tissue RAS rather than exerting a direct cytotoxic effect on tumor cells.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Vanadium-catalyzed chlorination under molecular oxygen
    • Abstract: Publication date: Available online 3 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Toshiyuki Moriuchi , Yasuhiro Fukui , Satoshi Kato , Tomomi Kajikawa , Toshikazu Hirao
      A catalytic chlorination of ketones was performed by using a vanadium catalyst in the presence of Bu4NI and AlCl3 under atmospheric molecular oxygen. This catalytic chlorination could be applied to the chlorination of alkenes to give the corresponding vic-dichlorides. AlCl3 was found to serve as both a Lewis acid and a chloride source to induce the facile chlorination. A combination of Bu4NI and AlI3 in the presence of a vanadium catalyst under atmospheric molecular oxygen induced the iodination of ketones.
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      PubDate: 2015-02-28T12:16:33Z
       
  • An NMR study on the
           6,6′-(2-(diethylamino)ethylazanediyl)bis(methylene)bis(5-hydroxy-2-hydroxymethyl-4H-pyran-4-one)
           interaction with AlIII and ZnII ions
    • Abstract: Publication date: Available online 2 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Massimiliano Peana , Serenella Medici , Valeria Marina Nurchi , Joanna Izabela Lachowicz , Guido Crisponi , Miriam Crespo-Alonso , Maria Amelia Santos , Maria Antonietta Zoroddu
      Here we report about the complex formation among an amine-bearing bis-kojic acid, 6,6′-(2-(diethylamino)ethylazanediyl)bis(methylene)bis(5-hydroxy-2-hydroxymethyl-4H-pyran-4-one) and two metal ions, the trivalent hard and not essential metal ion AlIII and the borderline and essential divalent metal ion ZnII. We carried out a thorough NMR study in order to reach the indispensable structural information on the behavior of these complexes in solution. A combination of 1D, 2D total correlation spectroscopy, heteronuclear single quantum coherence spectroscopy, nuclear Overhauser enhancement spectroscopy and rotating-frame Overhauser effect spectroscopy experiments was used to assign the signals of both free and metal-bound ligand at different pH values. Our results highlighted the different coordination behaviors of the ligand towards the different metal ions, depending on their hard or borderline character. The trivalent metal ion, AlIII, mainly forms dinuclear helicate complexes of M2L3 stoichiometry, and the coordination only involves both hydroxypyrone (O,O)-donor atoms. NMR data are in agreement with the presence of a rigid and symmetric structure of L9–AlIII complexes up to physiological pH. On the contrary, with the divalent metal ion, NMR data showed the coexistence of several species in solution though ZnII forms complexes of ML stoichiometry at physiological pH, where the metal coordination involves the nitrogen atoms of both the linker and the side-chain amine groups together with the oxygen atoms of phenolate groups. The in solution study will be of interest for providing an insight on the ligand bioavailability and on its behavior in the chelation treatments.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis, crystal and solution structures and antimicrobial screening of
           palladium(II) complexes with 2-(phenylselanylmethyl)oxolane and
           2-(phenylselanylmethyl)oxane as ligands
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): Zorica M. Bugarčić , Vera M. Divac , Marina D. Kostić , Nenad Ž. Janković , Frank W. Heinemann , Niko S. Radulović , Zorica Z. Stojanović-Radić
      Two novel Pd(II) complexes with 2-(phenylselanylmethyl)oxolane and 2-(phenylselanylmethyl)oxane as ligands were synthesized. The crystal and molecular structure of the complexes has been determined by single crystal X-ray diffraction. It turned out for both complexes that the two ligands are coordinated to Pd via Se atoms in a trans-fashion and the other two trans-positions are occupied by Cl ions. Detailed 1D- and 2D-NMR analyses revealed the existence of equilibrating trans-diastereomeric species differing in the configuration at four chiral centers (selenium and carbon) in the solution of the complexes. A computational study was also undertaken to assess the relative stabilities of the mentioned stereoisomeric species. The antimicrobial properties of the complexes were investigated against a series of human pathogenic bacterial and fungal strains. The complexes were shown to possess promising broad spectrum moderate antimicrobial activity that is more pronounced against fungal organisms. The noted activity could be completely attributed to the Pd(II) center, whereas the ligands probably mediate the transportation of a Pd(II) species across cell membranes.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Mn(II) complexes of scorpiand-like ligands. A model for the MnSOD active
           centre with high in vitro and in vivo activity
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143
      Author(s): M. Paz Clares , Carolina Serena , Salvador Blasco , Aida Nebot , Lucas del Castillo , Conxa Soriano , Antonio Domènech , Ana Virginia Sánchez-Sánchez , Laura Soler-Calero , José Luis Mullor , Antonio García-España , Enrique García-España
      Manganese complexes of polyamines consisting of an aza-pyridinophane macrocyclic core functionalised with side chains containing quinoline or pyridine units have been characterised by a variety of solution techniques and single crystal x-ray diffraction. Some of these compounds have proved to display interesting antioxidant capabilities in vitro and in vivo in prokaryotic (bacteria) and eukaryotic (yeast and fish embryo) organisms. In particular, the Mn complex of the ligand containing a 4-quinoline group in its side arm which, as it happens in the MnSOD enzymes, has a water molecule coordinated to the metal ion that shows the lowest toxicity and highest functional efficiency both in vitro and in vivo.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Editorial Board
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143




      PubDate: 2015-02-28T12:16:33Z
       
  • Contents
    • Abstract: Publication date: February 2015
      Source:Journal of Inorganic Biochemistry, Volume 143




      PubDate: 2015-02-28T12:16:33Z
       
  • Heteroditopic P,N ligands in gold(I) complexes: Synthesis, structure and
           cytotoxicity
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Telisha Traut-Johnstone , Stonard Kanyanda , Frederik H. Kriel , Tanya Viljoen , P.D. Riekert Kotze , Werner E. van Zyl , Judy Coates , D. Jasper G. Rees , Mervin Meyer , Raymond Hewer , D. Bradley G. Williams
      New heteroditopic, bi- and multidentate imino- and aminophosphine ligands were synthesised and complexed to [AuCl(THT)] (THT=tetrahydrothiophene). X-ray crystallography confirmed Schiff base formation in three products, the successful reduction of the imino-group to the sp3-hybridised amine in several instances, and confirmed the formation of mono-gold(I) imino- and aminophosphine complexes for four Au-complexes. Cytotoxicity studies in cancerous and non-cancerous cell lines showed a marked increase in cytotoxicity upon ligand complexation to gold(I). These findings were supported by results from the 60-cell line fingerprint screen of the Developmental Therapeutics Programme of the National Institutes of Health for two promising compounds. The cytotoxicity of some of these ligands and gold(I)complexes is due to the induction of apoptosis. The ligands and gold(I)complexes demonstrated selective toxicity towards specific cell lines, with Jurkat T cells being more sensitive to the cytotoxic effects of these compounds, while the non-cancerous human cell line KMST6 proved more resistant when compared to the cancerous cell lines. Results from the NIH DTP 60 cell-line fingerprint screen support the observed enhancement of cytotoxicity upon gold(I) complexation. One gold(I)complex induced high levels of apoptosis at concentrations of 50μM in all the cell lines screened in this study, while some of the other compounds selectively induced apoptosis in the cell lines. These results point towards the potential for selective toxicity to cancerous cells through the induction of apoptosis.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Cellular organization of siderophore biosynthesis in Pseudomonas
           aeruginosa: Evidence for siderosomes
    • Abstract: Publication date: Available online 4 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Véronique Gasser , Laurent Guillon , Olivier Cunrath , Isabelle.J. Schalk
      Pyoverdine I (PVDI) and pyochelin (PCH) are the two major siderophores produced by Pseudomonas aeruginosa PAO1 to import iron. The biochemistry of the biosynthesis of these two siderophores has been described in detail in the literature over recent years. PVDI assembly requires the coordinated action of seven cytoplasmic enzymes and is followed by a periplasmic maturation before secretion of the siderophore into the extracellular medium by the efflux system PvdRT-OpmQ. PCH biosynthesis also involves seven cytoplasmic enzymes but no periplasmic maturation. Recent findings indicate that the cytoplasmic enzymes involved in each of these two siderophore biosynthesis pathways can form siderophore-specific multi-enzymatic complexes called siderosomes associated with the inner leaflet of the cytoplasmic membrane. This organization may optimize the transfer of the siderophore precursors between the various participating enzymes and avoid the diffusion of siderophore precursors, able to chelate metals, throughout the cytoplasm. Here, we describe these recently published findings and discuss the existence of these siderosomes in P. aeruginosa.
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      PubDate: 2015-02-28T12:16:33Z
       
  • DNA damage and induction of apoptosis in pancreatic cancer cells by a new
           dinuclear bis(triazacyclonane) copper complex
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Diego Montagner , Valentina Gandin , Cristina Marzano , Andrea Erxleben
      The dinuclear copper(II) complex [Cu2{bcmp(-H)}(μ-OH)](NO3)2·H2O (1, bcmp=2,6-bis(1,4,7-triazacyclonon-1-ylmethyl)-4-methylphenol) has been synthesized and characterized by electrospray ionization mass spectrometry, potentiometric titration and cyclovoltammetry. The X-ray structure of the analogous perchlorate salt [Cu2{bcmp(-H)}(μ-OH)](ClO4)2·2.5H2O (2) was determined. Cytotoxicity studies showed very promising activity of 1 against various pancreatic tumor cell lines with IC50 values comparable or even lower than those of cisplatin. The Cu complex displayed low toxicity against a human non-tumor cell line (HEK 293) demonstrating selectivity for cancer cells. 1 converts supercoiled pUC19 plasmid DNA into the nicked form at micromolar concentrations in the absence of added reductants. A detailed kinetic study on the hydrolysis of the DNA model bis(2,4-dinitrophenyl) phosphate (BDNPP) has been performed. 1 hydrolyses BDNPP with a second order rate constant of 0.047Ms−1 at pH8 and 40°C. Finally, single cell electrophoresis (comet assay) and fluorescence microscopy analysis showed that 1 interacts with cellular DNA and induces apoptotic cell death of Capan-1 pancreatic cancer cells. Western blotting analysis indicated that the Cu complex activates the p53 dependent pathway of apoptosis.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Enhancing the copper(II) complexes cytotoxicity to cancer cells through
           bound to human serum albumin
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Yi Gou , Yao Zhang , Jinxu Qi , Zuping Zhou , Feng Yang , Hong Liang
      We use Schiff-base salicylaldehyde benzoylhydrazone (HL) as the ligand for copper(II), resulting in the complexes [CuCl(L)]·H2O (C1), [CuNO3(L)]·H2O (C2) and [CuBr(L)]2 (C3). We characterize the Cu(II) compounds' interactions with human serum albumin (HSA) using fluorescence spectroscopy and molecular docking. These studies revealed that Cu(II) compounds propensity bound to IIA subdomain of HSA possible by hydrophobic interactions and hydrogen bond. Cu(II) compounds produce intracellular reactive oxygen species (ROS) in cancer cells. Complexes of HSA and copper(II) compounds enhance about 2-fold cytotoxicity in cancer cells but do not raise cytotoxicity levels in normal cells in vitro. Compared with C3 alone, HSA–C3 complex promotes HepG2 cell apoptosis and has a stronger capacity to promote cell cycle arrest at the G2/M phase of HepG2.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis, structural studies and biological activity of new Cu(II)
           complexes with acetyl derivatives of 7-hydroxy-4-methylcoumarin
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Marcin T. Klepka , Aleksandra Drzewiecka-Antonik , Anna Wolska , Paweł Rejmak , Kinga Ostrowska , Elżbieta Hejchman , Hanna Kruszewska , Agnieszka Czajkowska , Izabela Młynarczuk-Biały , Wiesława Ferenc
      The new Cu(II) complexes with 6-acetyl-7-hydroxy-4-methylcoumarin (HL1) and 8-acetyl-7-hydroxy-4-methylcoumarin (HL2) have been obtained by the electrochemical method. The density functional theory calculations and X-ray absorption spectroscopy techniques have been used to geometrically describe a series of new compounds. The studies have been focused on the coordination mode of the hydroxy ligands to the metallic centre. The complexes, Cu(HL1)2 and Cu(HL2)2⋅0.5H2O, have flat square geometry with oxygen atoms in the first coordination sphere. Two bidentate anionic coumarins are bonded to the metal cation via the acetyl and deprotonated hydroxyl O atoms. Biological activity, including microbiological and cytotoxic, has been evaluated and found to be enhanced in comparison with the parent ligands. Moreover, the Cu(II) complex with 8-acetyl-7-hydroxy-4-methylcoumarin shows similar antifungal activity as commercially used fluconazole.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Ternary copper(II) complexes with amino acid chains and heterocyclic
           bases: DNA binding, cytotoxic and cell apoptosis induction properties
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Tieliang Ma , Jun Xu , Yuan Wang , Hao Yu , Yong Yang , Yang Liu , Weiliang Ding , Wenjiao Zhu , Ruhua Chen , Zhijun Ge , Yongfei Tan , Lei Jia , Taofeng Zhu
      Nowadays, chemotherapy is a common means of oncology. However, it is difficult to find excellent chemotherapy drugs. Here we reported three new ternary copper(II) complexes which have potential chemotherapy characteristics with reduced Schiff base ligand and heterocyclic bases (TBHP), [Cu(phen)(TBHP)]H2O (1), [Cu(dpz)(TBHP)]H2O (2) and [Cu(dppz)(TBHP)]H2O (3) (phen=1,10-phenanthroline, dpz=dipyrido [3,2:2′,3′-f]quinoxaline, dppz=dipyrido [3,2-a:2′,3′-c]phenazine, H2TBHP=2-(3,5-di-tert-butyl-2-hydroxybenzylamino)-2-benzyl-acetic acid). The DNA-binding properties of the complexes were investigated by spectrometric titrations, ethidium bromide displacement experiments and viscosity measurements. The results indicated that the three complexes, especially the complex 13, can strongly bind to calf-thymus DNA (CT-DNA). The intrinsic binding constants K b of the ternary copper(II) complexes with CT-DNA were 1.37×105, 1.81×105 and 3.21×105 for 1, 2 and 3 respectively. Comparative cytotoxic activities of the copper(II) complexes were also determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that the ternary copper(II) complexes had significant cytotoxic activity against the human lung cancer (A549), human esophageal cancer (Eca109) and human gastric cancer (SGC7901) cell lines. Cell apoptosis were detected by AnnexinV/PI flow cytometry and by Western blotting with the protein expression of p53, Bax and Bcl-2. All the three copper complexes can effectively induce apoptosis of the three human tumor cells.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis, structure and biological activity of nickel(II) complexes with
           mefenamato and nitrogen-donor ligands
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Xanthippi Totta , Aikaterini A. Papadopoulou , Antonios G. Hatzidimitriou , Athanasios Papadopoulos , George Psomas
      Six novel nickel(II) complexes with the non-steroidal anti-inflammatory drug mefenamic acid (Hmef) with the nitrogen-donor heterocyclic ligands 2,2′-bipyridine (bipy), 2,2′-bipyridylamine (bipyam), 1,10-phenanthroline (phen), 2,2′-dipyridylketone oxime (Hpko) or pyridine (py) and/or the oxygen-donor ligands CH3OH or H2O were synthesized and characterized by physicochemical and spectroscopic techniques. The crystal structures of [Ni(mef-O)2(bipy)(CH3OH)2] (1), [Ni(mef-O)2(phen)(CH3OH)2] (2), [Ni(mef-O,O′)2(bipyam)] (3) and [Ni(mef-O)2(Hpko)2]∙CH3OH (4·CH3OH) were determined by X-ray crystallography. The ability of the complexes to scavenge 1,1-diphenyl-picrylhydrazyl, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radicals was investigated and the in vitro inhibitory activity against soybean lipoxygenase was evaluated; complexes 3 and 4 were the most active compounds. Spectroscopic (UV and fluorescence), electrochemical (cyclic voltammetry) and physicochemical (viscosity measurements) techniques were employed in order to study the binding mode of the complexes to calf-thymus (CT) DNA and to calculate the corresponding binding constants; for all complexes, intercalation was the most possible mode of DNA-binding. The interaction of the complexes with serum albumins was studied by fluorescence emission spectroscopy and the values of the albumin-binding constants were determined.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Structural determination of Cu and Fe–Citrate complexes: theoretical
           investigation and analysis by ESI-MS
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Alexandre C. Bertoli , Ruy Carvalho , Matheus P. Freitas , Teodorico C. Ramalho , Daiana T. Mancini , Maria C. Oliveira , Amarílis de Varennes , Ana Dias
      The combined use of ESI-MS (electrospray ionization-mass spectrometry) and theoretical calculations for the determination of citrate:metal (metal=Cu and Fe) structures are reported. Mass spectrometry allowed to determine the stoichiometry 1:1 and 2:1 of the complexes, corroborating the theoretical calculations. The species found in the ratio 2:1 had their calculated structures readjusted, from what was originally simulated, since the deprotonation of citric acid differed from what was before simulated. The thermodynamic stability (ΔH(aq.) 0) of the complexes optimized at the B3LYP/LANL2DZ level was more exoenergetic than for the complexes found by the PM6 semi-empirical method.
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      PubDate: 2015-02-28T12:16:33Z
       
  • A ruthenium(II) complex inhibits tumor growth in vivo with fewer
           side-effects compared with cisplatin
    • Abstract: Publication date: Available online 14 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Jin-Quan Wang , Ping-Yu Zhang , Liang-Nian Ji , Hui Chao
      The antitumor activity of a ruthenium(II) polypyridyl complex, Δ-[Ru(bpy)2(HPIP)](ClO4)2 (Δ-Ru1, where bpy=2,2′-bipyridine, HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline), was evaluated. The in vivo experiments showed that Δ-Ru1 inhibited the growth of a human cervical carcinoma cell line (HeLa) xenotransplanted into nude mice with efficiency similar to that of cisplatin. Histopathology examination of the tumors from treated xenograft models was consistent with apoptosis in tumor cells. Importantly, in striking contrast with cisplatin, Δ-Ru1 did not cause any detectable side effects on the kidney, liver, peripheral neuronal system, or the hematological system at the pharmacologically effective dose. The preclinical studies reported here provide support for the clinical use of Δ-Ru1 as an exciting new drug candidate with lower toxicity than cisplatin, endowed with proapoptotic properties.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis, solution and crystal structure of the coenzyme B12 analogue
           Coβ-2′-fluoro-2′,5′-dideoxyadenosylcobalamin
    • Abstract: Publication date: Available online 16 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Miriam Hunger , Klaus Wurst , Bernhard Kräutler
      Crystal structure analyses have helped to decipher the mode of binding of coenzyme B12 (AdoCbl) in the active site of AdoCbl-dependent enzymes. However, the question of how such enzymes perform their radical reactions is still incompletely answered. A pioneering study by Gruber and Kratky of AdoCbl-dependent glutamate mutase (GLM) laid out a path for the movement of the catalytically active 5′-deoxyadenosyl radical, in which H-bonds between the protein and the 2′- and 3′-OH groups of the protein bound AdoCbl would play a decisive role. Studies with correspondingly modified coenzyme B12-analogues are of interest to gain insights into cofactor binding and enzyme mechanism. Here we report the preparation of Coβ-2′-fluoro-2′,5′-dideoxyadenosylcobalamin (2′FAdoCbl), which lacks the 2′-OH group critical for the interaction in enzymes. 2′FAdoCbl was prepared by alkylation of cob(I)alamin, obtained from the electrochemical reduction of aquocobalamin. Spectroscopic data and a single crystal X-ray analysis of 2′FAdoCbl established its structure, which was very similar to that one of coenzyme B12. 2′FAdoCbl is a 19F NMR active mimic of coenzyme B12 that may help to gain insights into binding interactions of coenzyme B12 with AdoCbl-dependent enzymes, proteins of B12 transport and of AdoCbl-biosynthesis, as well as with B12-riboswitches.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Prospects of molybdenum and rhenium octahedral cluster complexes as X-ray
           contrast agents
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Anna A. Krasilnikova , Michael A. Shestopalov , Konstantin A. Brylev , Irina A. Kirilova , Olga P. Khripko , Kristina E. Zubareva , Yuri I. Khripko , Valentina T. Podorognaya , Lidiya V. Shestopalova , Vladimir E. Fedorov , Yuri V. Mironov
      Investigation of new X-ray contrast media for radiography is an important field of science since discovering of X-rays in 1895. Despite the wide diversity of available X-ray contrast media the toxicity, especially nephrotoxicity, is still a big problem to be solved. The octahedral metal-cluster complexes of the general formula [{M6Q8}L6] can be considered as quite promising candidates for the role of new radiocontrast media due to the high local concentration of heavy elements, high tuning ability of ligand environment and low toxicity. To exemplify this, the X-ray computed tomography experiments for the first time were carried out on some octahedral cluster complexes of molybdenum and rhenium. Based on the obtained data it was proposed to investigate the toxicological proprieties of cluster complex Na2H8[{Re6Se8}(P(CH2CH2CONH2)(CH2CH2COO)2)6]. Observed low cytotoxic and acute toxic effects along with rapid renal excretion of the cluster complex evidence its perspective as an X-ray contrast media for radiography.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Connectivity patterns and rotamer states of nucleobases determine
           acid–base properties of metalated purine quartets
    • Abstract: Publication date: Available online 16 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Marc Sven Lüth , Eva Freisinger , Gunnar Kampf , Marta Garijo Anorbe , Rolf Griesser , Bert P. Operschall , Helmut Sigel , Bernhard Lippert
      Potentiometric pH titrations and pD dependent 1H NMR spectroscopy have been applied to study the acidification of the exocyclic amino group of adenine (A) model nucleobases (N9 position blocked by alkyl groups) when carrying trans-a2PtII (with a=NH3 or CH3NH2) entities both at N1 and N7 positions. As demonstrated, in trinuclear complexes containing central A-Pt-A units, it depends on the connectivity pattern of the adenine bases (N7/N7 or N1/N1) and their rotamer states (head-head or head-tail), how large the acidifying effect is. Specifically, a series of trinuclear complexes with (A-N7)-Pt-(N7-A) and (A-N1)-Pt-(N1-A) cross-linking patterns and terminal 9-alkylguanine ligands (9MeGH, 9EtGH) have been analyzed in this respect, and it is shown that, for example, the 9MeA ligands in trans-,trans-,trans-[Pt(NH3)2(N7-9MeA-N1)2{Pt(NH3)2(9EtGH-N7)}2](ClO4)6 .6H2O (4a) and trans-,trans-,trans-[Pt(NH3)2(N7-9EtA-N1)2{Pt(CH3NH2)2(9-MeGH-N7)}2](ClO4)6 .3H2O (4b) are more acidic, by ca. 1.3 units (first pK a), than the linkage isomer trans-,trans-,trans-[Pt(CH3NH2)2(N1-9MeA-N7)2{Pt(NH3)2(9MeGH-N7)}2](NO3)6 .6.25H2O (1b). Overall, acidifications in these types of complexes amount to 7–9 units, bringing the pK a values of such adenine ligands in the best case close to the physiological pH range. Comparison with pK a values of related trinuclear PtII complexes having different co-ligands at the Pt ions, confirm this picture and support our earlier proposal that the close proximity of the exocyclic amino groups in a head-head arrangement of (A-N7)-Pt-(N7-A), and the stabilization of the resulting N6H–…H2N6 unit, is key to this difference.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis, characterization, X-ray crystal structure, DFT calculation and
           antibacterial activities of new vanadium(IV, V) complexes containing
           chelidamic acid and novel thiourea derivatives
    • Abstract: Publication date: Available online 20 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Javad Farzanfar , Khaled Ghasemi , Ali Reza Rezvani , Hojat Samareh Delarami , Ali Ebrahimi , Hona Hosseinpoor , Amir Eskandari , Hadi Amiri Rudbari , Giuseppe Bruno
      Three new thiourea ligands derived from the condensation of aroyl- and aryl-isothiocyanate derivatives with 2,6-diaminopyridine, named 1,1'-(pyridine-2,6-diyl)bis(3-(benzoyl)thiourea) (L1), 1,1'-(pyridine-2,6-diyl)bis(3-(2-chlorobenzoyl)thiourea) (L2) and 1,1'-(pyridine-2,6-diyl)bis(3-(4-chlorophenyl)thiourea) (L3), their oxido-vanadium(IV) complexes, namely [VO(L1')(H2O)] (C1), [VO(L2')(H2O)] (C2) and [VO(L3')(H2O)] (C3), and also, dioxo-vanadium(V) complex containing 4-hydroxy-2,6-pyridine dicarboxylic acid (chelidamic acid, H2dipic-OH) and metformin (N,N-dimethylbiguanide, Met), named [H2Met][VO2(dipic-OH)]2 ·H2O (C4), were synthesized and characterized by elemental analysis, FTIR and 1H NMR and UV-visible spectroscopies. Proposed structures for free thiourea ligands and their vanadium complexes were corroborated by applying geometry optimization and conformational analysis. Solid state structure of complex [H2Met][VO2(dipic-OH)]2 ·H2O (triclinic, Pī) was fully determined by single crystal X-ray diffraction analysis. In this complex, metformin is double protonated and acted as counter ion. The antibacterial properties of these compounds were investigated in vitro against standard Gram-positive and Gram-negative bacterial strains. The experiments showed that vanadium(IV) complexes had the superior antibacterial activities than novel thiourea derivatives and vanadium(V) complex against all Gram-positive and Gram-negative bacterial strains.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Platinated oligomers of bovine pancreatic ribonuclease: Structure and
           stability
    • Abstract: Publication date: Available online 26 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Delia Picone , Federica Donnarumma , Giarita Ferraro , Irene Russo Krauss , Andrea Fagagnini , Giovanni Gotte , Antonello Merlino
      The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Physiological roles of peroxido-vanadium complexes: Leitmotif as their
           signal transduction pathway
    • Abstract: Publication date: Available online 25 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Seiichi Matsugo , Kan Kanamori , Hironori Sugiyama , Hirofumi Misu , Toshinari Takamura
      Evidence exists that supports the various physiological roles of vanadium compounds, although the amount of vanadium in our body is limited. This limited concentration in our body does not attract much attention of the biological chemists, although the fact is present, even in 19th century, vanadium derivatives were used for the therapeutic reagents. In the middle of 20th century, the main focus of vanadium chemistry is mainly on the chemical and material fields. After the first discovery of vanadium compounds expressing ATPase activity, oxidovanadium(IV) sulfate was reported to have insulin mimic activity. Additionally, because some vanadium compounds possess cellular toxicity, trials were also carried out to examine the possible use of vanadium compounds as cancer therapeutics. The application of vanadium complexes was extended in recent years especially in 21st century. In this review, we briefly explain the historical background of vanadium chemistry and also summarize the physiological role of vanadium complexes mainly focusing on the synthesis and physiological role of peroxidovanadium compounds and their interactions with insulin signal transduction pathways.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Vanadium(V) complex with Schiff-base ligand containing a flexible amino
           side chain: Synthesis, structure and reactivity
    • Abstract: Publication date: Available online 25 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Simona Nicafsu , Manfred Rudolph , Ines Lippold , Axel Buchholz , Helmar Görls , Winfried Plass
      The Schiff-base ligand (H2salhyhNH3)Cl ( 1 ) derived from salicylaldehyde and 6-aminohexanoic acid hydrazide hydrochloride reacts with ammonium metavanadate in methanol solution to yield the dioxidovanadium(V) complex [VO2(salhyhNH3)] ( 2 ). The utilized hydrazone ligand contains a flexible and protonated amino side chain. Crystallization from methanol affords complex 2 in the monoclinic space group P21/n, whereas crystallization from a methanol/water mixture 1:1 yields crystals, containing a water molecule of crystallization per two formula units ( 2 ⋅1/2H2O), in the orthorhombic space group Pbcn. In both cases the protonated amino group compensates the negative charge on the dioxidovanadium moiety and is involved in an extensive hydrogen bonding network particularly including the oxido groups from neighboring vanadium complexes. The reactivity of complex 2 toward protonation in aqueous solution has been investigated by spectrophotometric titrations and is characterized by two subsequent protonation steps at the hydrazide nitrogen atom of the ligand system and an oxido group leading to the formation of an oxidohydroxidovanadium(V) species with corresponding pK a values of 3.2 and 2.9, respectively. With larger excess of acid the oxidohydroxidovanadium(V) species starts to form the corresponding anhydride. The formation of the anhydride is strongly favored in the presence of methanol. The reaction of complex 2 with hydrogen peroxide in methanol solution leads the formation of an oxidoperoxidovanadium(V) species, whereas in aqueous solution the addition of one equivalent of acid is required. Complex 2 catalyzes the oxidation of methylphenylsulfane to the corresponding sulfoxide in methanol/dichloromethane mixture using hydrogen peroxide as oxidant at room temperature.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis and characterization of fluoride-incorporated polyoxovanadates
    • Abstract: Publication date: Available online 26 February 2015
      Source:Journal of Inorganic Biochemistry
      Author(s): Yuji Kikukawa , Taiga Yokoyama , Sanae Kashio , Yoshihito Hayashi
      The speciation studies of oxovanadates are essential to clarify their biological activities. We surveyed the distribution of oxovanadate species in the presence of halide anions with various acid concentrations in an aqueous mixed-solvent system. The presence of chloride, bromide, and iodide anions has no effects on the appearance of polyoxovanadate species observed in 51 V NMR. Those are the precedent formation of metavanadate species and decavanadates. The presence of fluoride anion during the addition of acids exhibits the strong intervention in the polyoxovanadate equilibria and we found the subsequent formation of two polyoxovanadate species by 51 V NMR observation. From the estimated experimental condition, we isolated fluoride-incorporated polyoxovanadates {Et4N}4[V7O19F] and {Et4N}4[HV11O29F2], successfully. Polyanion [V7O19F]4− is the fluoride-incorporated all V(V) state polyoxovanadate which has two different coordination environments of tetrahedral and square pyramidal vanadium units within the one anionic structural integrity. The structural gap between tetrahedral-unit-based metavanadate and octahedral-unit based decavanadate structure may be linked by this hybrid complex.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Chromium speciation and biochemical changes vary in relation to plant
           ploidy
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Jozef Kováčik , Bořivoj Klejdus , Petr Babula , Maria Elisa Soares , Josef Hedbavny , Maria de Lourdes Bastos
      Uptake of trivalent chromium (Cr(III)-chloride), Cr speciation and consequences for the metabolism in chamomile plants with two ploidy levels have been studied. Depletion of fresh biomass, tissue water content and soluble proteins in response to high (120μM) Cr(III) was ploidy-independent. Cr mainly accumulated in the roots (only negligibly in the shoots) and total root Cr amount was higher in tetraploid ones including the proof with specific fluorescent indicator (naphthalimide–rhodamine) of Cr(III). Quantification of Cr(VI) detected its higher content in tetraploid roots (up to 4.2% from total Cr), indicating partial oxidation of applied Cr(III). Higher H2O2 presence but lower activities of peroxidases were observed in tetraploid roots while nitric oxide, superoxide dismutase and glutathione reductase activities did not differ extensively. Soluble phenols, lignin, non-protein thiols, individual thiols (glutathione and phytochelatin 2) and ascorbic acid responded to high Cr(III) similarly in both cultivars while decrease of minerals was more pronounced in tetraploid ones. It seems that Cr(III)-induced oxidative stress arises from high root Cr uptake and Cr(VI) presence and is related to depletion of thiols. Assay of Krebs cycle acids confirmed rather depletion under 120μM Cr(III) in both cultivars but increase in citric acid may indicate its involvement in root Cr chelation. Subsequent comparison of Cr(III)-chloride and Cr(III)-nitrate showed similar influence on Cr accumulation and majority of biochemical responses while different impact on phytochelatin 2 amount was the most distinct feature.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis and characterization of water-soluble, heteronuclear
           ruthenium(III)/ferrocene complexes and their interactions with
           biomolecules
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Craig M. Anderson , Swapan S. Jain , Lisa Silber , Kody Chen , Sumedha Guha , Wancong Zhang , Emily C. McLaughlin , Yongfeng Hu , Joseph M. Tanski
      The reaction of Na[RuCl4(SO(CH3)2)2], 1, with one equivalent of FcCONHCH2C6H4N (Fc=FeC10H9), L1, FcCOOCH2CH2C3H3N2, L2, FcCOOC6H4N, L3, afforded the dinuclear species, Na[FcCONHCH2C6H4N[RuCl4(SO(CH3)2)]], RuL1, Na[FcCOOCH2CH2C3H3N2[RuCl4(SO(CH3)2)]], RuL2, Na[FcCOOC6H4N(RuCl4(SO(CH3)2))], RuL3, respectively, yielding, in each case, a ferrocene moiety bridged to a ruthenium center. The complexes were characterized by NMR, IR, and XRD (X-ray diffraction). The sulfoxide ligands are bonded to the metal through the sulfur atom. The complexes were evaluated for their biological activity with pBluescript DNA plasmid, and the protein BSA (bovine serum albumin). These reactions were monitored by XAS (X-ray absorption spectroscopy), EXAFS (extended X-ray Absorption Fine Structure), NMR, UV/visible, emission spectroscopy, and gel electrophoresis. Donor atoms from the biomolecules substitute for the chloride ligands in the parent complexes.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Bio-relevant cobalt(II) complexes with compartmental polyquinoline ligand:
           Synthesis, crystal structures and biological activities
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Jun-Ling Li , Lin Jiang , Bi-Wei Wang , Jin-Lei Tian , Wen Gu , Xin Liu , Shi-Ping Yan
      Three new Co(II) complexes, [Co4(L)2(μ 3-CrO4)2](ClO4)2 ·2CH3CN (1), [Co2(L)(μ 2-na)(H2O)](ClO4)2 (2) and [Co2(L)(μ 2-ba)](ClO4)2 ·0.5CH3CN (3) (Hna=nicotinic acid, Hba=benzoic acid, HL = N,N,N′,N′-tetrakis (2-quinolylmethyl)-1,3-diaminopropan-2-ol), have been synthesized and characterized by various physicochemical techniques. The Co(II) centers are connected by endogenous alkoxy bridge from L− and various extrinsic auxiliary linkers, some of which display coordination number asymmetry (5, 6-coordinated for 1 and 2; 5, 5-coordinated for 3). It is worth mentioning that complex 1 contains two rare reported μ 3-η 1, η 1, η 1-CrO4 2− moieties. Susceptibility data of three complexes indicated intramolecular antiferromagnetic coupling of high-spin Co(II) atoms with exchange integral values (J) −14.94cm−1, −11.26cm−1 and −13.66cm−1 for 1, 2 and 3, respectively. Interaction of compounds with calf thymus DNA (CT-DNA) have been investigated by absorption spectral titration, ethidium bromide (EB) displacement assay and viscosity measurement, which revealed that compounds bound to CT-DNA with a moderate intercalative mode, accompanied the affinities order: 1 > 2 ≈ 3. Three complexes exhibit oxidative cleavage of pBR322 plasmid DNA including a reliance on H2O2 as the activator. Compound 1 demonstrates an increased DNA cleavage activity as compared with 2 and 3, which could degrade super coiled DNA (SC DNA) into nicked coiled DNA (NC DNA) in lower concentration (5μM). Moreover, all compounds could quench the intrinsic fluorescence of bovine serum albumin (BSA) in a static quenching process. Complex 1 also shows higher anticancer activity than cisplatin with lower IC50 value of incubation for both 24h and 48h.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • pH-metric chemical speciation modeling and studies of in vitro
           antidiabetic effects of bis[(imidazolyl)carboxylato]oxidovanadium(IV)
           complexes
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Isaac Z. Gundhla , Ryan S. Walmsley , Vital Ugirinema , Nandipha O. Mnonopi , Eric Hosten , Richard Betz , Carminita L. Frost , Zenixole R. Tshentu
      A range of bidentate N,O-donor ligands of the imidazolyl-carboxylate moiety, which partially mimic naturally occurring bioligands, were prepared and reacted with the oxidovanadium(IV) ion to form the corresponding bis-coordinated oxidovanadium(IV) complexes. The aqueous pH-metric chemical speciation was investigated using glass electrode potentiometry, which allowed for the determination of protonation and stability constants of the ligands and complexes, respectively. The species distribution diagrams generated from this information gave evidence that the bis[(imidazolyl)carboxylato]oxovanadium(IV) complexes possess a broad pH-metric stability. The complexes improved glucose uptake in cell cultures using 3T3-L1 adipocytes, C2C12 muscle cells and Chang liver cells. The PTP inhibition studies indicated that the mechanism underlying insulin-stimulated glucose uptake was possibly via the protein tyrosine phosphorylation through the inhibition of the protein tyrosine phosphatase 1B (PTP 1B). The vanadium compounds also demonstrated the inhibition of D-dimer formation, suggesting that these compounds could potentially relieve a hypercoagulative state in diabetic patients.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Ferric microperoxidase-11 catalyzes peroxynitrite isomerization
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Paolo Ascenzi , Loris Leboffe , Roberto Santucci , Massimo Coletta
      Microperoxidase-11 (MP11) is an undecapeptide derived from horse heart cytochrome c offering the possibility to study the reactivity of the heme group relatively unshielded by the protein. Here, the peroxynitrite isomerization to NO3 − catalyzed by ferric MP11 (MP11–Fe(III)) is reported. Data were obtained between pH3.6 and 8.1, at 20.0°C. The value of the second-order rate constant (k on) for peroxynitrite isomerization to NO3 − by MP11–Fe(III) decreases from (1.1±0.1)×105 M−1 s−1, at pH3.6, to (6.1±0.6)×103 M−1 s−1, at pH8.1. The pH dependence of k on (pK a =6.9) suggests that peroxynitrous acid reacts preferentially with MP11–Fe(III). The MP11–Fe(III)-catalyzed isomerization of peroxynitrite to NO3 − has been ascribed to the reactive penta-coordinated heme–Fe atom of MP11–Fe(III). In fact, cyanide binding to the sixth coordination position of the heme–Fe atom inhibits the MP11–Fe(III)-catalyzed isomerization of peroxynitrite to NO3 −. The values of the first-order rate constant (k 0) for isomerization of peroxynitrite to NO3 − in the presence of the MP11–Fe(III)–CN complex are superimposable to those obtained in the absence of MP–Fe(III). Values of k on for peroxynitrite isomerization to NO3 − by MP11–Fe(III) overlap those obtained for penta-coordinated cardiolipin–cytochrome c complex and for carboxymethylated cytochrome c in absence and presence of cardiolipin. Present results highlight the role of the heme–Fe(III) co-ordination state in the modulation of cytochrome c reactivity.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Synthetic, potentiometric and spectroscopic studies of chelation between
           Fe(III) and 2,5-DHBA supports salicylate-mode of siderophore binding
           interactions
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Suheel K. Porwal , Emilia Furia , Michael E. Harris , Rajesh Viswanathan , L. Devireddy
      Catecholate type enterobactin, a prototype siderophore, comprises 2,3-dihydroxybenzoic acid (2,3-DHBA) cyclically linked to serine in E. coli. The existence of iron-chelating ligands in humans is a recent discovery, however, the basic chemical interactions between 2,5-dihydroxybenzoic acid and Fe(III) ion remain poorly understood. Achieving an accurate description of the fundamental Fe(III) binding properties of 2,5-DHBA is essential for understanding its role in iron transport mechanisms. Here, we show that 2,5-DHBA binds iron in a salicylate mode via a two-step kinetic mechanism by UV spectroscopy. Complexation between Fe(III) salt and 2,5-DHBA initially occurs at 1:1 ratio (of ligand to metal) and binding resulting in higher-order complexes continues at higher concentrations. Through potentiometric measurements we quantify the distribution of Fe(III)-2,5-DHBA complexes with 1:1, 1:2 and 1:3 stoichiometry. The formation of 1:3 complexes is further supported through high-resolution mass spectrometry. Further, using kinetic and equilibrium UV spectroscopy, we report Fe(III)-2,5-DHBA complex formation at a pH range of 2.5–9.0 at 298.15K in water. Maximum complexation occurred at a pH range of 4.5–6.5 consistent with deprotonation of the carboxylic acid proton. Equilibrium measurements and stopped-flow kinetics show that complexation rate constants were independent of concentrations of 2,5-DHBA. Together the data supports a model in which the rate-determining step involves rearrangement of ligands on an initial complex formed by reversible binding between the carboxylate group of 2,5-DHBA and Fe(III).
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Contents
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145




      PubDate: 2015-02-28T12:16:33Z
       
  • Sulfur and selenium antioxidants: Challenging radical scavenging
           mechanisms and developing structure–activity relationships based on
           metal binding
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145
      Author(s): Matthew T. Zimmerman , Craig A. Bayse , Ria R. Ramoutar , Julia L. Brumaghim
      Because sulfur and selenium antioxidants can prevent oxidative damage, numerous animal and clinical trials have investigated the ability of these compounds to prevent the oxidative stress that is an underlying cause of cardiovascular disease, Alzheimer's disease, and cancer, among others. One of the most common sources of oxidative damage is metal-generated hydroxyl radical; however, very little research has focused on determining the metal-binding abilities and structural attributes that affect oxidative damage prevention by sulfur and selenium compounds. In this review, we describe our ongoing investigations into sulfur and selenium antioxidant prevention of iron- and copper-mediated oxidative DNA damage. We determined that many sulfur and selenium compounds inhibit CuI-mediated DNA damage and that DNA damage prevention varies dramatically when FeII is used in place of CuI to generate hydroxyl radical. Oxidation potentials of the sulfur or selenium compounds do not correlate with their ability to prevent DNA damage, highlighting the importance of metal coordination rather than reactive oxygen species scavenging as an antioxidant mechanism. Additional gel electrophoresis, mass spectrometry, and UV–visible studies confirmed sulfur and selenium antioxidant binding to CuI and FeII. Ultimately, our studies established that both the hydroxyl-radical-generating metal ion and the chemical environment of the sulfur or selenium significantly affect DNA damage prevention and that metal coordination is an essential mechanism for these antioxidants.
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      PubDate: 2015-02-28T12:16:33Z
       
  • Editorial board
    • Abstract: Publication date: April 2015
      Source:Journal of Inorganic Biochemistry, Volume 145




      PubDate: 2015-02-28T12:16:33Z
       
  • Europium-doped Gd2O3 nanotubes cause the necrosis of primary mouse bone
           marrow stromal cells through lysosome and mitochondrion damage
    • Abstract: Publication date: May 2015
      Source:Journal of Inorganic Biochemistry, Volume 146
      Author(s): Yi Jin , Shizhu Chen , Jianlei Duan , Guang Jia , Jinchao Zhang
      With the wide applications of europium-doped Gd2O3 nanoparticles (Gd2O3:Eu3+ NPs) in biomedical fields, it will inevitably increase the chance of human exposure. It was reported that Gd2O3:Eu3+ NPs could accumulate in bone. However, there have been few reports about the potential effect of Gd2O3:Eu3+ NPs on bone marrow stromal cells (BMSCs). In this study, the Gd2O3:Eu3+ nanotubes were prepared and characterized by powder X-ray diffraction (XRD), photoluminescence (PL) excitation and emission spectra, scanning electron microscope (SEM), and transmission electron microscopy (TEM). The cytotoxicity of Gd2O3:Eu3+ nanotubes on BMSCs and the associated mechanisms were further studied. The results indicated that they could be uptaken into BMSCs by an energy-dependent and macropinocytosis-mediated endocytosis process, and primarily localized in lysosome. Gd2O3:Eu3+ nanotubes effectively inhibited the viability of BMSCs in concentration and time-dependent manners. A significant increase in the percentage of late apoptotic/necrotic cells, lactate dehydrogenase (LDH) leakage and the number of PI-stained cells was found after BMSCs were treated by 10, 20, and 40μg/mL of Gd2O3:Eu3+ nanotubes for 12h. No obvious DNA ladders were detected, but a dispersed band was observed. The above results revealed that Gd2O3:Eu3+ nanotubes could trigger cell death by necrosis instead of apoptosis. Two mechanisms were involved in Gd2O3:Eu3+ nanotube-induced BMSCs necrosis: lysosomal rupture and release of cathepsins B; and the overproduction of reactive oxygen species (ROS) injury to the mitochondria and DNA. The study provides novel evidence to elucidate the toxicity mechanisms and may be beneficial to more rational applications of these nanomaterials in the future.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • (Aminophosphane)gold(I) and silver(I) complexes as antibacterial agents
    • Abstract: Publication date: May 2015
      Source:Journal of Inorganic Biochemistry, Volume 146
      Author(s): Lourdes Ortego , Jesús Gonzalo-Asensio , Antonio Laguna , M. Dolores Villacampa , M. Concepción Gimeno
      This manuscript describes the synthesis of new Au(I) and Ag(I) complexes with aminophosphane ligands and a study of their antibacterial activity against Gram-negative Salmonella enterica serovar typhimurium and Escherichia coli and Gram-positive Listeria monocytogenes and Staphylococcus aureus. The bactericidal assays revealed the effectiveness of these compounds on paradigm Gram-negative and Gram-positive pathogens, showing a moderate antimicrobial activity, comparable with the antibiotics of reference, for all gold(I) complexes and the silver(I) complexes without coordinated PPh3 groups. For those complexes that were found to show inhibitory activity, serial dilutions in liquid broth method were performed for determination of MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration).
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Design, synthesis and anticancer activity of diam(m)ine platinum(II)
           complexes bearing a small-molecular cell apoptosis inducer dichloroacetate
           
    • Abstract: Publication date: May 2015
      Source:Journal of Inorganic Biochemistry, Volume 146
      Author(s): Weiping Liu , Jing Jiang , Yongping Xu , Shuqian Hou , Liping Sun , Qingsong Ye , Liguang Lou
      Four new diam(m)ine platinum complexes containing the dichloroacetate moiety in 3-dichoroacetoxylcyclobutane-1,1-dicarboxylate as the leaving group were synthesized, characterized by elemental analysis as well as by ESI+-MS (electrospray ionization mass spectrometry in positive mode), FT-IR, 1H- and 13C-NMR, and evaluated for their in vitro anticancer activity against human lung cancer cell line (A549) and ovarian cancer cell lines (SK-OV-3, SK-OV-3/DDP). Diam(m)ines used in the present study belong to the carriers of six clinically approved platinum drugs. Among the complexes synthesized, complex 2, cis-[Pt(II)(1R,2R-diaminocyclohexane)·(3-dichoroacetoxylcyclobutane-1,1-dicarboxylate)] is the most promising in terms of water solubility and potential of being totally devoid of cross-drug resistance with cisplatin. Therefore, complex 2 was selected for the dichloroacetate release test. The test shows dichloroacetate can be efficiently released from complex 2 under physiological conditions via the hydrolysis of an ester bond bridging the dichloroacetate moiety and platinum pharmacophores together. Our study supports the further evaluation of this complex as a drug candidate.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Macrophage and colon tumor cells as targets for a binuclear silver(I)
           N-heterocyclic carbene complex, an anti-inflammatory and apoptosis
           mediator
    • Abstract: Publication date: May 2015
      Source:Journal of Inorganic Biochemistry, Volume 146
      Author(s): Muhammad Adnan Iqbal , Muhammad Ihtisham Umar , Rosenani A. Haque , Mohamed B. Khadeer Ahamed , Mohd Zaini Bin Asmawi , Amin Malik Shah Abdul Majid
      Chronic inflammation intensifies the risk for malignant neoplasm, indicating that curbing inflammation could be a valid strategy to prevent or cure cancer. Cancer and inflammation are inter-related diseases and many anti-inflammatory agents are also used in chemotherapy. Earlier, we have reported a series of novel ligands and respective binuclear Ag(I)–NHC complexes (NHC= N-heterocyclic carbene) with potential anticancer activity. In the present study, a newly synthesized salt (II) and respective Ag(I)–NHC complex (III) of comparable molecular framework were prepared for a further detailed study. Preliminarily, II and III were screened against HCT-116 and PC-3 cells, wherein III showed better results than II. Both the compounds showed negligible toxicity against normal CCD-18Co cells. In FAM-FLICA caspase assay, III remarkably induced caspase-3/7 in HCT-116 cells most probably by tumor necrosis factor-alpha (TNF-α) independent intrinsic pathway and significantly inhibited in vitro synthesis of cytokines, interleukin-1 (IL-1) and TNF-α in human macrophages (U937 cells). In a cell-free system, both the compounds inhibited cyclooxygenase (COX) activities, with III being more selective towards COX-2. The results revealed that III has strong antiproliferative property selectively against colorectal tumor cells which could be attributed to its pro-apoptotic and anti-inflammatory abilities.
      Graphical abstract image

      PubDate: 2015-02-28T12:16:33Z
       
  • Synthesis and characterization of the anticancer and metal binding
           properties of novel pyrimidinylhydrazone derivatives
    • Abstract: Publication date: March 2015
      Source:Journal of Inorganic Biochemistry, Volume 144
      Author(s): Veronika F.S. Pape , Dóra Türk , Pál Szabó , Michael Wiese , Eva A. Enyedy , Gergely Szakács
      Three novel pyrimidinylhydrazones substituted at either the aromatic moiety or at the imine carbon atom were synthesized and characterized by standard analytical methods. All compounds were found to be toxic in the micro- to submicromolar range against a diverse panel of cancer cell lines including multidrug resistant (MDR) derivatives expressing P-glycoprotein (Pgp). UV–visible spectrophotometry experiments demonstrated that the most active compound (3) forms highly stable complexes with iron(III) and copper(II) in a wide pH range with a stronger preference towards iron(III). The redox activity of the iron and copper complexes of ligand 3 was investigated using cyclic voltammetry and was tested with cellular reductants. The impact of reactive oxygen species (ROS) on the mechanism of toxicity was assessed using the ROS-sensitive cell permeable dye 2′,7′-dichlorofluorescin diacetate (DCFDA). Our results demonstrate that the studied pyrimidinylhydrazones form redox-active iron and copper complexes that are capable of producing intracellular ROS, which might lead to cellular damage and cell death in cancer cells regardless of their resistance status.
      Graphical abstract image

      PubDate: 2015-01-27T20:50:43Z
       
 
 
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