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BIOTECHNOLOGY (236 journals)                  1 2 | Last

Showing 1 - 200 of 237 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 14)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 8)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 10)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 64)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 31)
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 8)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 43)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 63)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 8)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 5)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 155)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 5)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 13)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 16)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 16)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 2)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 56)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 12)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 13)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Biotechnology and Strategic Health Research     Open Access  
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 24)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 11)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 2)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 535)

        1 2 | Last

Journal Cover Journal of Trace Elements in Medicine and Biology
  [SJR: 0.576]   [H-I: 49]   [1 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0946-672X
   Published by Elsevier Homepage  [3162 journals]
  • Nickel-induced neurodegeneration in the hippocampus, striatum and cortex;
           an ultrastructural insight, and the role of caspase-3 and α-synuclein
    • Authors: Omamuyovwi M. Ijomone; Sunday Y. Olatunji; Joshua O. Owolabi; Thajasvarie Naicker; Michael Aschner
      Abstract: Publication date: December 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 50
      Author(s): Omamuyovwi M. Ijomone, Sunday Y. Olatunji, Joshua O. Owolabi, Thajasvarie Naicker, Michael Aschner
      Human overexposure to nickel (Ni) emanating from the increasing application of Ni compounds in modern technology is a major public health concern. Nickel has been shown to be teratogenic, immunotoxic, genotoxic and carcinogenic. The current knowledge on Ni neurotoxicity is still relatively limited. We have previously demonstrated that Ni treatment alters cognitive and locomotor behaviors, induces oxidative stress and neurodegeneration in brains of rats. In this study, we examine the ultrastructural changes to neurons in the hippocampus, striatum and cortex of the brain following Ni treatment, as well as attempt to delineate the roles for caspase-3 and α-synuclein in Ni-induced neurodegeneration. Rats were treated with either saline, 10 or 20 mg/kg of nickel chloride for 4 weeks via oral gavage. Electron microscopy analysis revealed ultrastructural alterations in neurons of the hippocampus, striatum and cortex following Ni treatment. Mitochondria structural integrity within neurons were markedly compromised. We also detected elevated caspase-3 activity in hippocampus and striatum, as well as overexpression of α-synuclein in the cortex following Ni treatment. Our study demonstrates that mitochondria are a key target in Ni-induced neurodegeneration. Additionally, we implicate apoptotic pathway via caspase-3 action as the executioner and perturbation of α-synuclein expression in Ni-induced neurodegeneration.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.017
      Issue No: Vol. 50 (2018)
  • Development, validation and application of an ICP-MS/MS method to quantify
           minerals and (ultra-)trace elements in human serum
    • Authors: Sören Meyer; Mariya Markov; Gabriele Poh; Talke A. Marschal; Olga Pivovarov; Andreas F.H. Pfeiffe; Tanja Schwerdtle
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Sören Meyer, Mariya Markova, Gabriele Pohl, Talke A. Marschall, Olga Pivovarova, Andreas F.H. Pfeiffer, Tanja Schwerdtle
      Multi-element determination in human samples is very challenging. Especially in human intervention studies sample volumes are often limited to a few microliters and due to the high number of samples a high-throughput is indispensable. Here, we present a state-of-the-art ICP-MS/MS-based method for the analysis of essential (trace) elements, namely Mg, Ca, Fe, Cu, Zn, Mo, Se and I, as well as food-relevant toxic elements such as As and Cd. The developed method was validated regarding linearity of the calibration curves, method LODs and LOQs, selectivity and trueness as well as precision. The established reliable method was applied to quantify the element serum concentrations of participants of a human intervention study (LeguAN). The participants received isocaloric diets, either rich in plant protein or in animal protein. While the serum concentrations of Mg and Mo increased in participants receiving the plant protein-based diet (above all legumes), the Se concentration in serum decreased. In contrast, the animal protein-based diet, rich in meat and dairy products, resulted in an increased Se concentration in serum.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.012
      Issue No: Vol. 49 (2018)
  • Elemental changes of hippocampal formation occurring during postnatal
           brain development
    • Authors: J. Chwiej; M. Palczynska; A. Skoczen; K. Janeczko; J. Cieslak; R. Simon; Z. Setkowicz
      Pages: 1 - 7
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): J. Chwiej, M. Palczynska, A. Skoczen, K. Janeczko, J. Cieslak, R. Simon, Z. Setkowicz
      In this paper the elemental changes of rat hippocampal formation occurring during the postnatal development were examined. Three groups of animals were used in the study. These were naive Wistar rats at the age of 6-, 30- and 60-days and the chosen life periods corresponded to the neonatal period, childhood and early adulthood in humans, respectively. For the topographic and quantitative elemental analysis X-ray fluorescence microscopy was applied and the measurements were done at the FLUO beamline of ANKA. The detailed quantitative and statistical analysis was done for four areas of hippocampal formation, namely sectors 1 and 3 of the Ammon’s horn (CA1 and CA3, respectively), dentate gyrus (DG) and its internal area (hilus of DG, H). The obtained results showed that among the all examined elements (P, S, K, Ca, Fe, Cu, Zn and Se), only the levels of Fe and Zn changed significantly during postnatal development of the hippocampal formation and both the elements were significantly higher in young adults comparing to the rats in neonatal period. The increased Fe areal density was found in all examined hippocampal areas whilst Zn was elevated in CA3, DG and H. In order to follow the dynamics of age-dependent elemental changes, the statistical significance of differences in their accumulation between subsequent moments of time was examined. The obtained results showed statistically relevant increase of Zn level only in the first observation period (between 6th and 30th day of life). Afterwards the areal density of the element did not change significantly. The increase of Fe areal density took place in both examined periods, however the observed changes were small and usually not statistically relevant.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.030
      Issue No: Vol. 49 (2018)
  • Effect of various calcium salts on non-heme iron bioavailability in fasted
           women of childbearing age
    • Authors: Valeria Candia; Israel Ríos-Castillo; Frank Carrera-Gil; Berta Vizcarra; Manuel Olivares; Sotiris Chaniotakis; Fernando Pizarro
      Pages: 8 - 12
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Valeria Candia, Israel Ríos-Castillo, Frank Carrera-Gil, Berta Vizcarra, Manuel Olivares, Sotiris Chaniotakis, Fernando Pizarro
      Introduction Micronutrient deficiencies are one of the most important public health issues worldwide and iron (Fe) deficiency anemia is the most prevalent micronutrient deficiency. Iron deficiency often coexists with calcium deficiency and iron and calcium supplementation often overlap. This has led to investigations into the interaction between these two minerals, and whether calcium may inhibit iron absorption in the gut. Objective To determine the effect of various calcium salts on non-heme iron bioavailability in fasted women of childbearing age. Methods A randomized and single blinded trial was conducted on 27 women of childbearing age (35–45 years old) divided into 2 groups (n1 = 13 and n2 = 14, respectively). On four different days, after an overnight fast, they received 5 mg of Fe as FeSO4 (labeled with 55Fe or 59Fe) with 800 mg of elemental calcium in the form of either calcium chloride, calcium gluconate, calcium citrate, calcium carbonate, calcium lactate, calcium sulfate or calcium phosphate. Calcium chloride was used as the control salt in both groups. Iron was labeled with the radioisotopes 59Fe or 55Fe, and the absorption of iron was measured by erythrocyte incorporation of radioactive Fe Results 800 mg of elemental calcium as calcium citrate produced a significant decrease in non-heme iron bioavailability (repeated measures ANOVA, F = 3.79, p = 0.018). Conclusion Of the various calcium salts tested, calcium citrate was the only salt that decreased non-heme iron bioavailability relative to the calcium chloride control when taken on an empty stomach. These results suggest that inhibition of non-heme iron absorption in fasted individuals is dependent upon the calcium salt in question and not solely dependent on the presence of calcium.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.029
      Issue No: Vol. 49 (2018)
  • Trace determination of cobalt in biological fluids based on
           preconcentration with a new competitive ligand using dispersive
           liquid-liquid microextraction combined with slotted quartz tube–flame
           atomic absorption spectrophotometry
    • Authors: Elif Öztürk Er; Emine Gülhan Bakırdere; Tuğçe Unutkan; Sezgin Bakırdere
      Pages: 13 - 18
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Elif Öztürk Er, Emine Gülhan Bakırdere, Tuğçe Unutkan, Sezgin Bakırdere
      A new competitive ligand has been synthesized for the preconcentration to obtain lower detection limits by using dispersive liquid-liquid microextraction combined with slotted quartz tube-flame atomic absorption spectrophotometry (DLLME-SQT-FAAS). The proposed method is simple, eco-friendly and has high sensitivity. The preconcentration procedure was optimized on the basis of various parameters affecting the complex formation and extraction efficiency such as pH and volume of buffer solution, volume of ligand solution, mixing period, volume and type of extraction solvent, volume and type of dispersive solvent, and salt effect. Instrumental parameters were also optimized to get higher sensitivity. Under the optimum conditions, the calibration graph was linear in the range of 10–250 ng mL−1and the resulted limits of detection and quantification (LOD and LOQ) for combined method were 4.7 and 15.7 ng mL−1, respectively. The detection power was improved 48-fold using DLLME-SQT-FAAS method compared to conventional FAAS. The precision of the method was found to be high with a relative standard deviation of 2.5%. The accuracy of method was evaluated by recovery experiments using matrix matching study on spiked urine and blood samples. The recoveries for urine and blood samples ranged from 99.8 to 108.9% and 102.5 to 110.0%, respectively.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.027
      Issue No: Vol. 49 (2018)
  • Validation of a dilute and shoot method for quantification of 12 elements
           by inductively coupled plasma tandem mass spectrometry in human milk and
           in cow milk preparations
    • Authors: Stéphane Dubascoux; Daniel Andrey; Mario Vigo; Peter Kastenmayer; Eric Poitevin
      Pages: 19 - 26
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Stéphane Dubascoux, Daniel Andrey, Mario Vigo, Peter Kastenmayer, Eric Poitevin
      Nutritional information about human milk is essential as early human growth and development have been closely linked to the status and requirements of several macro- and micro-elements. However, methods addressing whole mineral profiling in human milk have been scarce due in part to their technical complexities to accurately and simultaneously measure the concentration of micro- and macro-trace elements in low volume of human milk. In the present study, a single laboratory validation has been performed using a “dilute and shoot” approach for the quantification of sodium (Na), magnesium (Mg), phosphorus (P), potassium (K), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), selenium (Se), molybdenum (Mo) and iodine (I), in both human milk and milk preparations. Performances in terms of limits of detection and quantification, of repeatability, reproducibility and trueness have been assessed and verified using various reference or certified materials. For certified human milk sample (NIST 1953), recoveries obtained for reference or spiked values are ranged from 93% to 108% (except for Mn at 151%). This robust method using new technology ICP-MS/MS without high pressure digestion is adapted to both routinely and rapidly analyze human milk micro-sample (i.e. less than 250 μL) in the frame of clinical trials but also to be extended to the mineral profiling of milk preparations like infant formula and adult nutritionals.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.023
      Issue No: Vol. 49 (2018)
  • Differential expression of zinc transporters accompanies the
           differentiation of C2C12 myoblasts
    • Authors: Amanda L. Paskavitz; Julia Quintana; Daniella Cangussu; Cristina Tavera-Montañez; Yao Xiao; Sonia Ortiz-Miranda; Juan G. Navea; Teresita Padilla-Benavides
      Pages: 27 - 34
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Amanda L. Paskavitz, Julia Quintana, Daniella Cangussu, Cristina Tavera-Montañez, Yao Xiao, Sonia Ortiz-Miranda, Juan G. Navea, Teresita Padilla-Benavides
      Zinc transporters facilitate metal mobilization and compartmentalization, playing a key role in cellular development. Little is known about the mechanisms and pathways of Zn movement between Zn transporters and metalloproteins during myoblast differentiation. We analyzed the differential expression of ZIP and ZnT transporters during C2C12 myoblast differentiation. Zn transporters account for a transient decrease of intracellular Zn upon myogenesis induction followed by a gradual increase of Zn in myotubes. Considering the subcellular localization and function of each of the Zn transporters, our findings indicate that a fine regulation is necessary to maintain correct metal concentrations in the cytosol and subcellular compartments to avoid toxicity, maintain homeostasis, and for loading metalloproteins needed during myogenesis. This study advances our basic understanding of the complex Zn transport network during muscle differentiation.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.024
      Issue No: Vol. 49 (2018)
  • Expression of zinc transporters ZIP4, ZIP14 and ZnT9 in hepatic
           carcinogenesis—An immunohistochemical study
    • Authors: Laura Gartmann; Thomas Wex; Kurt Grüngreiff; Dirk Reinhold; Thomas Kalinski; Peter Malfertheiner; Kerstin Schütte
      Pages: 35 - 42
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Laura Gartmann, Thomas Wex, Kurt Grüngreiff, Dirk Reinhold, Thomas Kalinski, Peter Malfertheiner, Kerstin Schütte
      Introduction Dysregulation of both, systemic zinc levels and tissue-specific zinc transporters, is reported in chronic inflammatory and malignant liver disease (hepatocellular carcinoma, HCC). Aim of this study is to assess the expression level of three zinc transporters in liver tissue and HCC: ZIP4, ZIP14 and ZnT9. Methods The study is based on tissue samples obtained from 138 patients with histologically proven HCC. Tissue specimens from tumor (n = 138) and extra-lesional specimens (n = 72) were assessed immunohistochemically for the expression of the three zinc transporters. Expression levels were semi-quantitatively scored and statistically analyzed with respect to the etiology of HCC (alcohol, AFLD; non-alcoholic fatty liver disease, NAFLD; virus-hepatitis, VH) and survival. Results Overall, expression levels of ZIP4, ZIP14 and ZnT9 were significantly higher in HCC tissue than in adjacent extra-lesional liver tissue. Expression levels in tumor tissue and survival time revealed a negative correlation for ZIP4 and ZIP14, and in part for ZnT9 (nuclear staining) (p < 0.05), whereas cytoplasmic staining of ZnT9 did not correlate with survival. Furthermore, the expression level of ZIP4 in extra-lesional tissue showed inverse correlation with survival time. Conclusion The upregulation of zinc transporters in hepatic carcinogenesis and its negative correlation with survival time implies a regulatory/functional link between zinc-homeostasis and development/progression of HCC that deserves to be further explored.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.034
      Issue No: Vol. 49 (2018)
  • Biomarkers of selenium status and antioxidant effect in workers
           occupationally exposed to mercury
    • Authors: Renata Kuras; Edyta Reszka; Edyta Wieczorek; Ewa Jablonska; Jolanta Gromadzinska; Beata Malachowska; Lucyna Kozlowska; Magdalena Stanislawska; Beata Janasik; Wojciech Wasowicz
      Pages: 43 - 50
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Renata Kuras, Edyta Reszka, Edyta Wieczorek, Ewa Jablonska, Jolanta Gromadzinska, Beata Malachowska, Lucyna Kozlowska, Magdalena Stanislawska, Beata Janasik, Wojciech Wasowicz
      The present observation based research was designed to evaluate the influence of occupational human exposure to metallic mercury (Hg°) vapor on the biomarkers of selenium status involved in the antioxidant defense system. For this purpose we determined Hg and selenium (Se) concentrations in body fluids, the markers of antioxidant effect measured as an activity of Se-dependent enzymes (red blood cell and plasma glutathione peroxidase: GPx1-RBC and GPx3-P), concentration of selenoprotein P in the plasma (SeP-P) and total antioxidant activity in the plasma (TAA-P) in 131 male workers from a chloralkali plant exposed to Hg° and 67 non-exposed males (control group). The mRNA expression levels of glutathione peroxidases (GPX1, GPX3), selenoprotein P (SEPP1), thioredoxin reductase 1 (TRXR1), thioredoxin 1 (TRX1), peroxiredoxins (PRDX1, PRDX2) were also examined in the leukocytes of peripheral blood. Hg concentration in the blood (Hg-B) and urine (Hg-U) samples was determined using the thermal decomposition amalgamation/atomic absorption spectrometry (TDA-AAS) method and Se concentrations in plasma (Se-P) and urine (Se-U) using the inductively coupled plasma mass spectrometry (ICP-MS) method. Activities of GPx1-RBC, GPx3-P and TAA-P were determined using the kinetic and spectrophotometric method, respectively. Gene expression analysis was performed using the quantitative Real-Time PCR. The results showed significant higher Hg levels among the Hg°–exposed workers in comparison to control group (12-times higher median for Hg-B and almost 74-times higher median for Hg-U concentration in chloralkali workers). Se-P was also significantly higher (Me (median): 82.85 μg/L (IQR (interquartile range) 72.03–90.28 μg/L) for chloralkali workers vs. Me: 72.74 μg/L (IQR 66.25-80.14 μg/L) for control group; p = 0.0001) but interestingly correlated inversely with Hg-U in chloralkali workers suggesting depletion of the Se protection among the workers with the highest Hg-U concentration. The mRNA level for GPX1, PRXD1 were markedly but significantly higher in the workers compared to the control group. Moreover, concentrations of Hg-B and Hg-U among the workers were significantly positively correlated with the levels of selenoprotein P at both the mRNA and selenoprotein levels. In the multivariate model, after adjusting to cofounders (dental amalgam fillings, age, BMI, job seniority time, smoking), we confirmed that Hg-U concentration was inversely correlated with genes expression of TRXR1. This is the first comprehensive assessment of the impact of occupational exposure of workers to Hg° at both the mRNA and selenoprotein levels, with investigation of fish intake obtained by means of a questionnaire. These findings suggest that exposure to Hg° alters gene expression of the antioxidant enzymes and the level of Se-containing selenoproteins.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.032
      Issue No: Vol. 49 (2018)
  • Prolonged stimulation of insulin release from MIN6 cells causes zinc
           depletion and loss of β-cell markers
    • Authors: Rebecca Lawson; Wolfgang Maret; Christer Hogstrand
      Pages: 51 - 59
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Rebecca Lawson, Wolfgang Maret, Christer Hogstrand
      Zinc is integral for the normal function of pancreatic β-cells in glycaemic control. Large amounts of zinc are secreted from β-cells following insulin exocytosis and regulated replenishment is required, which is thought to be mediated by the ZIP family of zinc importer proteins. Within Type 2 Diabetic patients, β-cells are stressed through prolonged stimulation by hyperglycaemia and this is thought to be a major factor contributing to loss of β-cell identity and mass. However, the consequences for the β-cell zinc status remain largely unexplored. We used inductively coupled plasma mass spectrometry (ICP-MS) to show that 24 h treatment of MIN6 cells with potassium chloride, mimicking hyperglycaemic stimulation, reduces the total cellular zinc content 2.8-fold, and qPCR to show an increase in mRNA expression for metallothioneins (Mt1 and Mt2) following 4 and 24 h of stimulation, suggestive of an early rise in cytosolic zinc. To determine which ZIP paralogues may be responsible for zinc replenishment, we used immunocytochemistry, Western blot and qPCR to demonstrate initial ZIP1 protein upregulation proceeded by downregulation of mRNA coding for ZIP1, ZIP6, ZIP7 and ZIP14. To assign a biological significance to the decreased total cellular zinc content, we assessed expression of key β-cell markers to show downregulation of mRNA for MafA, Mnx-1, Nkx2.2 and Pax6. Our data suggest hyperglycaemia-induced zinc depletion may contribute to loss of β-cell markers and promote β-cell dedifferentiation through disrupting expression of key transcription factors.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.020
      Issue No: Vol. 49 (2018)
  • Selectivity and sensitivity of molybdenum oxide-polycaprolactone nanofiber
           composites on skin cancer: Preliminary in-vitro and in-vivo implications
    • Authors: Indrakumar Janani; Rachita Lakra; Manikantan Syamala Kiran; Purna Sai Korrapati
      Pages: 60 - 71
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Indrakumar Janani, Rachita Lakra, Manikantan Syamala Kiran, Purna Sai Korrapati
      Cancer nanomedicine has emerged as a revolution in the last decade opening up promising strides for the cancer treatment. The major challenge in these therapeutic approaches resides in the failure of clinical trials owing to the immunological cancer microenvironment. Therefore, the success of next generation nanomedicine depends on tunable physicochemical nanomaterial design and corresponding clinical trials by integrating targeted delivery with mitigated toxicity. The present study deals with the fabrication of nanofibrous scaffold impregnated with molybdenum nanoparticles for targeted skin cancer therapeutics. Molybdenum oxide, a transitional metal oxide is gaining rapid importance due to its vital role in cellular and molecular metabolism. Polycaprolactone nanofibers were chosen as a matrix to localize the nanoparticles topically facilitating selective apoptosis of the tumor cells over the normal cells with mitigated side effects. The scaffold was designed to tailor the physicochemical, mechanical and biological suitability for skin cancer (melanoma and non melanoma). The designed scaffold was found to reduce more than 50% cell viability of the cancer cells selectively through apoptosis as confirmed using AO/PI staining and the probable mechanism could be attributed to the induction of mitochondria dependent apoptosis as observed by JC1 dye staining. In-vivo trials in zebra fish were found to reduce cancer progression by more than 30% in 14 days. The fabricated molybdenum trioxide nano constructs not only serve as tunable targeted systems but also open venues capable of ferrying chemotherapeutic drugs sparing normal cells alleviating the trauma due to side effects.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.033
      Issue No: Vol. 49 (2018)
  • Compounds containing trace element copper or zinc exhibit as potent
           hyperuricemia inhibitors via xanthine oxidase inactivation
    • Authors: Lan-Zhu Li; Guo-Xiu Zhou; Jia Li; Wei Jiang; Bao-Lin Liu; Wen Zhou
      Pages: 72 - 78
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Lan-Zhu Li, Guo-Xiu Zhou, Jia Li, Wei Jiang, Bao-Lin Liu, Wen Zhou
      Compounds containing trace elements copper or zinc are potential gout and hyperuricemia suppressant by virtue of their inhibiting effect on xanthine oxidase/xanthine dehydrogenase (XOD/XDH) and anti-inflammatory and anti-oxidative function. In this study, compounds Cu(hmy-paa)·SO4·H2O (simplified as CuHP) and Zn(hmy-paa)·SO4·H2O (simplified as ZnHP) are synthesized, where hmy-paa stands for 3-(4-hydroxy-3-methoxyphenyl)-N-(1H-pyrazol-3-yl)acrylamide). The ligand hmy-paa is composed of functional ferulic acid and 3-aminopyrazole. The XOD and XDH activity of the mouse liver homogenate could efficiently be inhibited by CuHP and ZnHP. XOD has been recognized as one of the promising targets for the treatment of hyperuricemia. Fluorescence spectrometry study indicates that the interaction between the compound and XOD could be strengthened by the introduction of metals. In vitro drug efficacy study illustrates that metals copper and zinc distinctly improves the uric acid reducing efficacy by suppressing XOD activation. Hyperuricemia mouse model is induced by co-treatment of hypoxanthine and oteracil potassium. Intraperitoneal injection of CuHP and ZnHP to hyperuricemia mice exhibits a significant effect on reducing serum uric acid. The serum creatinine value detection indicates that the side effect of CuHP and ZnHP on renal function is weak. The computational docking simulation exhibits the tightly binding mode between the compound and XOD. Consequently, compounds CuHP and ZnHP are new type candidates for the treatment of gout and hyperuricemia.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.019
      Issue No: Vol. 49 (2018)
  • Obesity, diabetes and zinc: A workshop promoting knowledge and
           collaboration between the UK and Israel, november 28–30, 2016 – Israel
    • Authors: Alicia Jenkins; Imre Lengyel; Guy A. Rutter; Nicola Lowe; Iris Shai; Amir Tirosh; Tunde Petro; Mogher Khamaisi; Simon Andrews; Niv Zmora; Atan Gross; Wolfgang Maret; Eli C. Lewis; Arie Moran
      Pages: 79 - 85
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Alicia Jenkins, Imre Lengyel, Guy A. Rutter, Nicola Lowe, Iris Shai, Amir Tirosh, Tunde Petro, Mogher Khamaisi, Simon Andrews, Niv Zmora, Atan Gross, Wolfgang Maret, Eli C. Lewis, Arie Moran

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.021
      Issue No: Vol. 49 (2018)
  • Effect of anti-rheumatic treatment on selenium levels in inflammatory
    • Authors: Gia Deyab; Ingrid Hokstad; Jan Aaseth; Milada Cvancarova Småstuen; Jon Elling Whist; Stefan Agewall; Torstein Lyberg; Dag Tveiten; Gunnbjorg Hjeltnes; Kazem Zibara; Ivana Hollan
      Pages: 91 - 97
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Gia Deyab, Ingrid Hokstad, Jan Aaseth, Milada Cvancarova Småstuen, Jon Elling Whist, Stefan Agewall, Torstein Lyberg, Dag Tveiten, Gunnbjorg Hjeltnes, Kazem Zibara, Ivana Hollan
      Objectives The reason for increased cardiovascular risk in inflammatory arthritis (IA) is unclear. Interestingly, selenium-deficiency is suspected to contribute to the development of cardiovascular disease (CVD) in the general population. Although the reference range of serum selenium (s-selenium) is 50–120 μg/L, there are indications that levels up to 85 μg/L might not be sufficient for optimal cardioprotection. Our aim was to examine s-selenium levels in rheumatoid arthritis (RA), psoriatic arthritis (PsA) and ankylosing spondylitis (AS), to evaluate the effect of anti-rheumatic treatment on s-selenium levels, and to assess relationships between s-selenium levels and clinical and laboratory parameters including markers of disease activity and CVD risk. Methods We examined 64 patients with RA, 40 with PsA and 26 with AS starting with methotrexate (MTX) monotherapy or anti-tumor necrosis factor therapy (anti-TNF) with or without methotrexate (anti-TNF ± MTX) due to active disease. S-selenium, inflammatory biomarkers, endothelial function (EF) and other variables were examined at baseline and after 6 weeks and 6 months of treatment. Results In the total IA group, s-selenium increased within 6 weeks of anti-rheumatic treatment, and thereafter the levels remained stable until the end of the 6 months follow-up period. There were no significant differences in s-selenium changes between the three diagnostic groups and between the two treatment regimens. Changes in s-selenium were negatively related to changes in C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR), but there were no significant relationships to any other of the examined risk parameters for CVD including EF. Conclusion IA patients had s-selenium within the reference range, but below the level that might be necessary for optimal CVD protection. Anti-rheumatic treatment had a relatively rapid and sustained effect on s-selenium levels. The increase in s-selenium was related to reduction in inflammatory activity. In theory, anti-rheumatic drugs might improve s-selenium levels through inhibition of pro-inflammatory processes or through other mechanisms. Although we have not revealed any significant relationships between s-selenium and CVD risk parameters, the role of suboptimal s-selenium levels in pathogenesis of premature CVD in IA cannot be ruled out.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.001
      Issue No: Vol. 49 (2018)
  • Evaluation of the uptake, storage and cell effects of nano-iron in
           enterocyte-like cell models
    • Authors: Daniel Turiel-Fernández; Jörg Bettmer; Maria Montes-Bayón
      Pages: 98 - 104
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Daniel Turiel-Fernández, Jörg Bettmer, Maria Montes-Bayón
      The therapy with nanocompounds is widely used to treat Fe deficiency and an emerging trend to inhibit tumor growth. The present work aims to address the management of different FeONP, comparing sucrose covered FeONP and Fe nanoparticles in the form of the ferritin with non-particulated inorganic Fe (II) by enterocytes-like colon cancer cell lines (Caco-2 and HT-29). Iron uptake results revealed significantly higher Fe incorporation in the case of nanoparticulated Fe, first in the form of FeONP and second in the form of ferritin with respect to inorganic Fe (II). Furthermore, the intracellular Fe fractionation, conducted by size exclusion chromatography coupled on line to inductively coupled plasma mass spectrometry (SEC-ICP-MS) showed a significant increase of the Fe-ferritin peak upon exposure of cells to the following compounds ferritin > FeONP > FeSO4. Such results point out that the sucrose coated FeONP released Fe into the cell cytosol that was used to replenish the existing cytosolic ferritin without inducing changes in the protein concentration. On the other hand, the increase of the Fe-ferritin peak in cells exposed to ferritin as iron source is due to a significant increase on the intracellular protein concentration, as proved by using an ICP-MS linked ferritin sandwich immune assay. Cell viability experiments conducted with concentrations up to 1000 μmol L−1 (as Fe) of each compound under scrutiny did not reveal significant differences among Fe species regarding global cellular toxicity. However, significant cell DNA damage was detected when treating the cells with FeONP (500 μmol L−1).
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.002
      Issue No: Vol. 49 (2018)
  • Zn-net special issue
    • Authors: Nicola Lowe; Lothar Rink
      First page: 105
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Nicola Lowe, Lothar Rink

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.035
      Issue No: Vol. 49 (2018)
  • The role of zinc in calprotectin expression in human myeloid cells
    • Authors: Simone Lienau; Lothar Rink; Inga Wessels
      Pages: 106 - 112
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Simone Lienau, Lothar Rink, Inga Wessels
      Elevated levels of calprotectin and other inflammatory mediators have been observed in inflammatory diseases paralleling serum hypozincemia. While a role of zinc in the regulation of tumor necrosis factor α, interleukin (IL)-1β and IL-6 expression has been established, the direct interrelation of zinc and calprotectin (S100A8/S100A9 heterodimer) expression is so far missing. In the present study, we analyzed mRNA and protein levels of S100A8 and S100A9 in monocytic Mono Mac (MM)1 and early myeloid THP-1 and U937 cells to elucidate the effect of zinc deficiency on their expression. We could depict that zinc deficiency alone enhances mRNA and protein expression of calprotectin in myeloid cells, independently from maturity stage. Moreover, pre-existing zinc deficiency augmented lipopolysaccharide (LPS)-induced calprotectin expression in CD14+ MM1, but not in CD14− U937 or CD14− THP-1 cells. Zinc deficiency and LPS seem therefore to activate different intracellular pathways. Our findings suggest that zinc does not only regulate the activity of calprotectin but also its expression by human myeloid cells.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.022
      Issue No: Vol. 49 (2018)
  • Effect of administration route and dose on metabolism of nine
    • Authors: Kazuaki Takahashi; Noriyuki Suzuki; Yasumitsu Ogra
      Pages: 113 - 118
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Kazuaki Takahashi, Noriyuki Suzuki, Yasumitsu Ogra
      The nutritional availability of selenium (Se) is highly dependent on its chemical form because chemical form affects absorption, distribution, metabolism, and excretion. We evaluated the effects of administration route and dose on the bioavailability of nine Se compounds found in biota, the so-called bioselenocompounds, such as selenite, selenate, selenocyanate (SeCN), Se-methylselenocysteine (MeSeCys), selenomethionine (SeMet), selenohomolanthionine (SeHLan), selenocystine (SeCys2), 1β-methylseleno-N-acetyl-d-galactosamine (SeSug1), and trimethylselenonium ion (TMSe). We determined the bioavailability of bioselenocompounds recovered as urinary selenometabolites and serum selenoproteins from urine and serum of Se-deficient rats after the administration of bioselenocompounds by speciation analysis. Urinary Se was more easily recovered than serum selenoproteins, suggesting that the speciation of urinary Se is a better tool to indicate Se status in the body. The intravenous administration of bioselenocompounds showed different Se bioavailability from the oral administration. Intestinal microflora might be involved in the bioavailability of some bioselenocompounds, such as SeCN, MeSeCys, and SeSug1.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.007
      Issue No: Vol. 49 (2018)
  • High spatial resolution LA-ICP-MS demonstrates massive liver copper
           depletion in Wilson disease rats upon Methanobactin treatment
    • Authors: Jennifer-Christin Müller; Josef Lichtmannegger; Hans Zischka; Michael Sperling; Uwe Karst
      Pages: 119 - 127
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Jennifer-Christin Müller, Josef Lichtmannegger, Hans Zischka, Michael Sperling, Uwe Karst
      Wilson disease (WD) is a rare genetic disorder of the copper metabolism leading to systemic copper accumulation, predominantly in the liver. The therapeutic approach in WD patients is the generation of a negative copper balance and the maintenance of copper homeostasis, currently by the use of copper chelators such as D-penicillamine (D-PA). However, in circumstances of delayed diagnosis, poor treatment compliance, or treatment failure, mortality is almost certain without hepatic transplantation. Moreover, even after years of D-PA treatment, high liver copper levels are present in WD patients. We have recently suggested the use of the bacterial peptide Methanobactin (MB), which has an outstanding binding affinity for copper, as potentially efficient and patient-friendly remedy against copper damage in WD. Here we substantiate these findings considerably, by demonstrating a significant removal of copper from liver samples of WD rats upon short, one week only, MB treatments. Using laser ablation-inductively coupled plasma-mass spectrometry with a spatial resolution down to 4 μm, we demonstrate that only small copper hotspots remain in MB treated animal livers. We further demonstrate in WD rat liver, seven weeks after the stopped MB treatment, a lower liver copper concentration as compared to untreated control animals. Thus, MB highly efficiently depletes liver copper overload with a sustained therapeutic effect.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.009
      Issue No: Vol. 49 (2018)
  • Iron localization in the guinea pig choroid plexus: A light and
           transmission electron microscopy study
    • Authors: Marilda da Cruz Fernandes; Antônio Generoso Severino; Adriana Maria Zago; Lucas Tortorelli; Fabiano B. Carvalho; Felipe Luis Schneider
      Pages: 128 - 133
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Marilda da Cruz Fernandes, Antônio Generoso Severino, Adriana Maria Zago, Lucas Tortorelli, Fabiano B. Carvalho, Felipe Luis Schneider
      The role of the choroid plexus (CP) in iron (Fe) homeostasis has been suggested as the main mechanism of Fe uptake and storage in the mammalian central nervous system. Thus, the CP of the lateral and fourth ventricles was studied in guinea pigs with light and electron microscopy using methods including Perls’ Prussian blue and Gomori acid phosphatase staining, immunoreactivity for ferritin and transferrin, as well as energy dispersive spectrometry microanalysis. The present study reveals the presence of endogenous Fe in CP epithelial cells. Under light microscopy, Prussian blue staining revealed dark blue precipitates (i.e., Fe3+) with a preferentially perinuclear localization. The Fe was also positive for such granules with similar cellular localization. Ultrastructural analysis demonstrated the presence of dense bodies and siderosomes with molecular ferritin. The spectra obtained by the microanalysis demonstrated emissions for Fe, both in dense bodies and siderosomes. This study suggests that guinea pig CP epithelial cells accumulate Fe in the form of ferritin, possibly in cytoplasmic organelles such as lysosomes.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.006
      Issue No: Vol. 49 (2018)
  • Influence of boric acid on energy metabolism and stress tolerance of
           Candida albicans
    • Authors: Martin Schmidt; Dominic Tran-Nguyen; Patrick Chizek
      Pages: 140 - 145
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Martin Schmidt, Dominic Tran-Nguyen, Patrick Chizek
      Boron presents at physiological pH in the form of boric acid (BA), a molecule that has both positive and negative effects on biological processes. In medicine, BA is used as a topical treatment for vaginal yeast infections by Candida species because of its well-documented but poorly understood effect on inhibition of growth in general and of invasive, hyphal growth in particular. The present study examines the influence of BA on carbohydrate energy metabolism of this common human pathogen. Starting from previous findings about an inhibition of key NAD-dependent enzymes by BA in vitro, we confirmed that such an inhibition occurs in permeabilized C. albicans cells. Cultures growing even with moderate concentrations of BA experience mitochondrial failure, increase ethanol production from glucose and decrease the deposition of carbohydrate stores in the form of glycogen. Cells growing on the non-fermentable, FAD-generating carbon source lactate have a higher BA tolerance, which suggests that the toxicity of BA is rooted in an inhibition of NAD-dependent reactions and the increased production of ethanol. Boric acid exposure sensitizes C. albicans selectively to the toxic effects of ethanol. This additive effect suggests that the endogenously produced ethanol increases the load on ethanol resistance mechanisms. Lastly, combination studies showed no interactions of BA with common antifungal drugs, meaning that addition of BA to topical formulations can provide an additive antifungal effect regardless of the chosen active ingredient.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.011
      Issue No: Vol. 49 (2018)
  • The effects of various levels of boron supplementation on live weight,
           plasma lipid peroxidation, several biochemical and tissue antioxidant
           parameters of male mice**
    • Authors: Varol Kurtoglu; Firuze Kurtoglu; Pınar Peker Akalin
      Pages: 146 - 150
      Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): Varol Kurtoglu, Firuze Kurtoglu, Pınar Peker Akalin
      In this study, live weight, live weight gain, plasma GPx, GR, LDH, ALT activities, triglyceride, total protein, albumin and LPO levels, also liver and brain SOD and GPx activities were investigated after administration of boron (0.10, 0.20 and 0.30 mg/day) into male mice with drinking water for 60 days. Blood albumin and triglyceride levels were not affected with boron (p > 0.05) where triglyceride levels, with increasing amounts of boron, displayed a slight decrease within the normal ranges. From the antioxidant-oxidant balance parameters, LPO and GR levels were not affected from boron, where GPx activity was increased significantly (p < 0.001) comparing the groups of boron and control. LDH and ALT activities were affected significantly (p < 0.001) with decreased ALT and increased LDH levels with increasing amounts of boron. In regards of liver and brain GPx and SOD activities, significant increases were determined. Liver GPx and SOD activities were increased within the groups with the increasing amount of boron, where in brain, SOD (p < 0.05) was affected significantly but GPx (p > 0.05) displayed a gradual insignificant increase. As regards live weight gain, a gradual increase was determined during experimental period, but only the 45th day, the increase was statistically significant (p < 0.05). It is suggested that, new studies on the effects of different doses and compounds of boron in laboratory animals in regards of antioxidant and metabolic effects may be helpful for the understanding of the subject.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.05.013
      Issue No: Vol. 49 (2018)
  • Serum concentrations of trace elements and their relationships with
           paraoxonase-1 in morbidly obese women
    • Authors: Fedra Luciano-Mateo; Noemí Cabré; Martí Nadal; Anabel García-Heredia; Gerard Baiges-Gaya; Anna Hernández-Aguilera; Jordi Camps; Jorge Joven; José Luis Domingo
      Pages: 8 - 15
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Fedra Luciano-Mateo, Noemí Cabré, Martí Nadal, Anabel García-Heredia, Gerard Baiges-Gaya, Anna Hernández-Aguilera, Jordi Camps, Jorge Joven, José Luis Domingo
      The metabolic alterations associated with obesity include mineral dysregulation. Essential trace elements are nutrients with a relevant function in a large number of cellular processes and multiple roles in the correct functioning of metabolic enzymes. Paraoxonase-1 (PON1) is an antioxidant and anti-inflammatory enzyme that is compromised in obesity. In the present study, the potential alterations in trace elements in morbidly obese women were assessed in relation to serum PON1 activity and concentration, as well as to other obesity-related comorbidities such as diabetes mellitus and fatty liver. We recruited 41 morbidly obese women and 51 control individuals. The serum concentrations of 30 elements, PON1 paraoxonase and lactonase activities, and PON1 concentration were measured. We observed significant alterations in the levels of As, Ba, Cu, Ca, Fe, Mg, Na, Se, Sr, and Zn in obese women; some of them (As, Ca, Cr, Cu, Mg, and Se) being significantly correlated with serum PON1 values. The most relevant changes were observed in the concentrations of As, Sr and Mg, the last of which was also significantly associated with diabetes mellitus. The current results raise the possibility that increased ingestion and/or storage of a number of trace elements may be factors predisposing to obesity-related comorbidities and metabolic alterations.

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.023
      Issue No: Vol. 48 (2018)
  • Essential elements in synovial fluid samples obtained from patients living
           in Northern Poland
    • Authors: Pawel Konieczynski; Grzegorz Szreder; Ewelina Tamowska; Marek Wesolowski
      Pages: 20 - 24
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Pawel Konieczynski, Grzegorz Szreder, Ewelina Tamowska, Marek Wesolowski
      Many people after 50 year of life suffer pains caused by osteoarthritis (OA), which make their life difficult and painful. There is a probable association of OA with the concentration of certain elements in body fluids. Therefore the aim of the study was to relate the level of essential elements in synovial fluids of patients with OA with the treatment using antiarthritic drugs. Thus, flame-atomic absorption spectrometry was used to determine the concentrations of Fe, Zn, Cu, Mn, Na, K, Ca and Mg in 74 samples of synovial fluids taken from 36 patients. Prior to analysis, the samples of synovial fluids were centrifuged and obtained supernatants were digested by microwave technique. It was found that the level of investigated essential elements was differentiated in patients suffering with OA. High correlation between Zn and K, Na and K, as well as Cu and Mn were revealed. Based on cluster analysis, synovial fluids of patients treated with Diprophos, Euflexxa and Synocrom, can be found almost in all clusters. ANOVA has shown that there is a significant difference between Na level in synovial fluids of patients treated with antirheumatic drugs, and those who were not treated. For the patients treated with Euflexxa, similar relations for Ca and K exist. Principal component analysis has shown that essential elements responsible for variability within studied samples, are Ca, Mg, Cu, Mn and Fe. However, there were no statistically significant relations between the studied macro- and microelements, and treatment using specific preparations.

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.027
      Issue No: Vol. 48 (2018)
  • Effects of furosemide administration on the concentration of essential and
           toxic elements in Wistar rats by inductively coupled plasma optical
           emission spectrometry
    • Authors: Luiz Raimundo Seneterri Silva Rodrigues dos Santos; Aníbal de Freitas Santos; Maria das Graças Andrade Korn
      Pages: 25 - 29
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Luiz Raimundo Seneterri Silva Rodrigues dos Santos, Aníbal de Freitas Santos, Maria das Graças Andrade Korn
      Furosemide can interfere with the metabolism of chemical elements, changing their levels in several tissues, thus causing imbalance. In this study, inductively coupled plasma optical emission spectrometry (ICP OES) was used for multi-element analysis (Cd, Cu, Fe, Mg, Pb, Se and Zn) after microwave-assisted digestion, to evaluate the effect of furosemide (loop diuretic) on the composition of these essential and toxic elements in biological samples (liver, kidney, heart, lung and serum) of Wistar rats. Male and female Wistar rats (n = 40, 180–350 g) were randomly divided into 2 groups (n = 20/group). The results were expressed as μg/g dry weight. The mean tissue concentrations (minimum-maximum in μg/g) of Cu, Fe, Mg and Zn in the biological samples ranged between 5.2 and 1023.5. The levels of Cd, Pb and Se were below the detection limit of the ICP OES. Accuracy was assessed by microwave-assisted digestion and recovery values of 83–116% were obtained. Liver had significantly higher trace element concentrations in most of the analyzed samples. Mg showed a significant reduction (for males and females) in its levels in the heart. In both genders, there was similarity in the Cu concentration reduction (around 16%) for all tissues. The highest iron losses were found for serum (52% and 12%) for male and female rats, respectively. Reductions in Zn occurred between 0.3 and 18.0%, mainly for kidneys and heart, respectively. This study demonstrated that furosemide altered the concentration of some elements in rats.

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.029
      Issue No: Vol. 48 (2018)
  • Boron intake, osteocalcin polymorphism and serum level in postmenopausal
    • Authors: Olcay Boyacioglu; Seda Orenay-Boyacioglu; Hatice Yildirim; Mehmet Korkmaz
      Pages: 52 - 56
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Olcay Boyacioglu, Seda Orenay-Boyacioglu, Hatice Yildirim, Mehmet Korkmaz
      The relationship between daily boron intake and osteocalcin-mediated osteoporosis was studied in boron-exposed postmenopausal women. It is known that boron and osteocalcin are important in bone metabolism, however the effect of boron in bone metabolism has not been fully discovered. The study was performed on 53 postmenopausal women aged 55–60 living in parts of Balikesir, Turkey, where the subjects are naturally exposed to high (≥1 mg/L) or low (<1 mg/L) boron concentration in drinking water. 24-h urine samples were collected from all participants and creatinine clearance was detected. Boron intake levels of the subjects whose clearance levels were between 80–124 mL/min were measured by inductively coupled plasma-optical emission spectrometry (ICP-OES) in urine samples. Serum osteocalcin levels of the subjects were measured by osteocalcin enzyme-linked immunosorbent assay (ELISA) kit. Osteocalcin polymorphism rs1800247 was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Serum osteocalcin levels in boron-exposed postmenopausal women were significantly higher than that of control group (P ≤ 0.05) and the correlation between the serum osteocalcin level and rs1800247 polymorphism was not significant in both groups (P > 0.05). The differences in the distribution of osteocalcin genotypes and alleles in postmenopausal women were not significant between the boron exposed and the control groups (P > 0.05). Serum osteocalcin level in the CC genotype was significantly higher compared to the TC genotype in boron-exposed group (P ≤ 0.05). Our study suggests that daily boron intake of 1 mg/L may affect bone metabolism in postmenopausal women positively.

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.03.005
      Issue No: Vol. 48 (2018)
  • Origin of thyroid dysfunction at the Saharawi refugee camps
    • Authors: N. Pichel; M. Vivar
      First page: 57
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): N. Pichel, M. Vivar

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.03.006
      Issue No: Vol. 48 (2018)
  • Reconsideration of the immunotherapeutic pediatric safe dose levels of
    • Authors: James Lyons-Weiler; Robert Ricketson
      Pages: 67 - 73
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): James Lyons-Weiler, Robert Ricketson
      FDA regulations require safety testing of constituent ingredients in drugs (21 CFR 610.15). With the exception of extraneous proteins, no component safety testing is required for vaccines or vaccine schedules. The dosing of aluminum in vaccines is based on the production of antibody titers, not safety science. Here we estimate a Pediatric Dose Limit that considers body weight. We identify several serious historical missteps in past analyses of provisional safe levels of aluminum in vaccines, and provide updates relevant to infant aluminum exposure in the pediatric schedule considering pediatric body weight. When aluminum doses are estimated from Federal Regulatory Code given body weight, exposure from the current vaccine schedule are found to exceed our estimate of a weight-corrected Pediatric Dose Limit. Our calculations show that the levels of aluminum suggested by the World Health Organization place infants at risk of acute, repeated, and possibly chronic exposures of toxic levels of aluminum in modern vaccine schedules. Individual adult exposures are on par with Provisional Tolerable Weekly Intake “limits”, but some individuals may be aluminum intolerant due to genetics or previous exposures. Vaccination in neonates and low birth-weight infants must be re-assessed; other implications for the use of aluminum-containing vaccines, and additional limitations in our understanding of neurotoxicity and safety levels of aluminum in biologics are discussed.

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.02.025
      Issue No: Vol. 48 (2018)
  • Health risk assessment of arsenic in Realgar and NiuHuangJieDu Tablets
           based on pharmacokinetic study
    • Authors: Xiao Wu; Shanhu Wu; Yuexin Liu; Rong Guan; Fangmei Liang; Min Song; Taijun Hang
      Pages: 81 - 86
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Xiao Wu, Shanhu Wu, Yuexin Liu, Rong Guan, Fangmei Liang, Min Song, Taijun Hang
      NiuHuangJieDu Tablets (NHJDT), a popular realgar (As4S4) containing patented traditional Chinese medicine (TCM), is widely used in the treatment of acute tonsillitis, pharyngitis, periodontitis and mouth ulcer. However, arsenic is considered as one of the most toxic elements, leading to growing concerns about the quality and safety of realgar-containing TCMs recently. In this study, health risk assessment of arsenic in realgar and NHJDT was conducted through oral administration of both substances to rats with single and multiple doses, respectively. The total blood arsenic concentration was used as the health risk indicator and determined by hydride generation-atomic fluorescence spectrometry after modified Kjeldahl digestion, and then applied to the pharmacokinetic study. For single oral dose study in rats, the low, medium, and high doses of realgar and NHJDT were set equivalent to 1, 5 and 20 times the human therapeutic dose (1.3 mg realgar/kg), respectively. Multiple doses were given at low and high dose levels every 12 h for seven consecutive days, respectively. Significant differences in the total blood arsenic pharmacokinetic profiles were observed between the corresponding realgar and NHJDT groups. These results indicated that NHJDT significantly reduced the total blood arsenic exposure present in realgar, and the detoxification mechanism might be attributed to herb–herb interactions in NHJDT. However, the accumulation of blood total arsenic was significant due to the long elimination half-life and high accumulation index in both realgar and NHJDT groups. Therefore, the potential health risk of arsenic caused by the administration of realgar-containing TCMs should be taken into account for excessive or long-term medication. Precautions should be taken for the clinical application of realgar-containing TCMs.
      Graphical abstract image

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.03.012
      Issue No: Vol. 48 (2018)
  • Determination of toxic and essential trace elements in serum of healthy
           and hypothyroid respondents by ICP-MS: A chemometric approach for
           discrimination of hypothyroidism
    • Authors: Aleksandar Stojsavljević; Jelena Trifković; Zorica Rasić-Milutinović; Dragana Jovanović; Gradimir Bogdanović; Jelena Mutić; Dragan Manojlović
      Pages: 134 - 140
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Aleksandar Stojsavljević, Jelena Trifković, Zorica Rasić-Milutinović, Dragana Jovanović, Gradimir Bogdanović, Jelena Mutić, Dragan Manojlović
      Inductively coupled plasma-mass spectrometry ((ICP-MS)) was used to determine three toxic (Ni, As, Cd) and six essential trace elements (Cr, Mn, Co, Cu, Zn, Se) in blood serum of patients with hypothyroidism (Hy group) and healthy people (control group), in order to set the experimental conditions for accurate determination of a unique profile of these elements in hypothyroidism. Method validation was performed with standard reference material of the serum by varying the sample treatment with both standard and collision mode for analysis of elements isotopes. Quadratic curvilinear functions with good performances of models and the lowest detection limits were obtained for 52Cr, 66Zn, 75As, 112Cd in collision mode, and 55Mn, 59Co, 60Ni, 65Cu, 78Se in standard mode. Treatment of serum samples with aqueous solution containing nitric acid, Triton X-100 and n-butanol gave the best results. Chemometric tools were applied for discrimination of patients with hypothyroidism. All nine elements discriminated Hy group of samples with almost the same discriminating power as indicated by their higher values for this group of patients. Statistically significant correlation (p < 0.01) was observed for several elements. Results indicated clear differences in element profile between Hy and control group and it could be used as a unique profile of hypothyroid state.

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.03.020
      Issue No: Vol. 48 (2018)
  • Seleno-l-methionine and l-ascorbic acid differentiate the biological
           activity of doxorubicin and its metal complexes as a new anticancer drugs
    • Authors: Marzena Matejczyk; Grzegorz Świderski; Renata Świsłocka; Stanisław Józef Rosochacki; Włodzimierz Lewandowski
      Pages: 141 - 148
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Marzena Matejczyk, Grzegorz Świderski, Renata Świsłocka, Stanisław Józef Rosochacki, Włodzimierz Lewandowski
      The most important problems of anti-cancer therapy include the toxicity of the drugs applied to healthy cells and the multi-drug cells resistance to chemotherapeutics. One of the most commonly used anticancer drugs is doxorubicin (DOX) used to treat certain leukemias and non-Hodgkin’s lymphomas, as well as bladder, breast, stomach, lung, ovarian, thyroid, multiple myeloma and other cancers. Preliminary studies showed that metal complex with DOX improve its cytostatic activity with changes in their molecular structure and distribution of electrons, resulting in a substantial change of its biological activity (including antitumor activity). Thus, there is a chance to receiving derivatives of DOX with low toxicity for the healthy body cells, thus increasing its therapeutic selectivity. In the present study we examined the influence of Mn, Mg, Fe, Co and Ni, seleno-l-methionine and vitamin C on biological activity of DOX in prokaryotic model - Escherichia coli RFM443, with plasmid transcriptional fusion of recA promoter and luxCDABE as a reporter gene. Cytotoxic potency of tested chemicals was calculated on the basis of the bacteria culture growth inhibition (GI%) values. Genotoxic properties were calculated on the basis of the fold increase (FI) of relative luminescence units (RLU) values compared to control. Obtained results showed that doxorubicin metal complexes particularly with Ni, Co and Fe increased the cyto- and genotoxic activities of DOX. Bacteria culture supplemented with SeMet and vitamin C differentiate the DOX and its metal complexes toxicity. It seems, that DOX-Ni, DOX-Fe and DOX-Co complexes could be potent cytostatic drug candidates. Moreover, we noticed different sensitivity of recA::luxCDABE for 3 h and 24 h cultures of bacteria strain. It suggests, that the potency of genetic construct reactivity- recA::luxCDABE in E. coli depends on the growth-phase of bacterial culture.
      Graphical abstract image

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.03.021
      Issue No: Vol. 48 (2018)
  • Effects of zinc, magnesium, and chromium supplementation on
           cardiometabolic risk in adults with metabolic syndrome: A double-blind,
           placebo-controlled randomised trial
    • Authors: Ha-Na Kim; Se-Hong Kim; Young-Mi Eun; Sang-Wook Song
      Pages: 166 - 171
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Ha-Na Kim, Se-Hong Kim, Young-Mi Eun, Sang-Wook Song
      The prevalence of metabolic syndrome (MetS) has been increasing rapidly worldwide. The activities of zinc, magnesium and chromium have a potential association with MetS; therefore, we investigated the effects of zinc, magnesium and chromium supplements on metabolic risk factors in adults with MetS. In this double-blind, placebo controlled randomised study, 32 adults with MetS were included in the zinc, magnesium, and chromium-administered group (n = 16) or the placebo group (n = 16) and received either 300 mg magnesium, 600 μg chromium and 36 mg zinc per day or placebo over a 24-week period. The primary endpoint was the change in the MetS components, including serum glucose, triglyceride and high-density lipoprotein cholesterol levels, blood pressure and waist circumference. Data were analysed using repeated-measures analysis of variance. The metabolic risk factors did not change post-intervention, but the serum C-reactive protein level decreased in the mineral-supplemented group compared with that in the placebo group. Further studies with stricter inclusion criteria are needed to better evaluate the potential for zinc, magnesium and chromium to improve metabolic risk in adults with MetS.

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.03.022
      Issue No: Vol. 48 (2018)
  • Participation of selenoproteins localized in the ER in the processes
           occurring in this organelle and in the regulation of
           carcinogenesis-associated processes
    • Authors: Elena Gennadyevna Varlamova
      Pages: 172 - 180
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Elena Gennadyevna Varlamova
      The functions performed by the ER are diverse: synthesis of steroid hormones, synthesis of proteins for the plasma membrane, lysosomes, as well as proteins meant for exocytosis, protein folding, formation of disulfide bonds, N-linked glycosylation, etc. Selenoproteins localized in this organelle are definitely involved in the processes occurring in it, and the most common of them include participation in protein degradation, regulation of ER stress and redox metabolism. ER stress has been registered in many types of cancer cells. The ability to persist under prolonged ER stress increases their survival, resistance to drugs and immunity. Disturbances in the redox regulation of the cell cycle, which result in the accumulation of misfolded proteins in the ER, viral infection, disruption of Ca2+ regulation, are known to cause an evolutionarily conserved reaction – unfolded protein response (UPR) and, ultimately, lead to ER stress. Since selenoproteins, as oxidoreductases, possess antioxidant properties, and their role in the regulation of important processes, such as carcinogenesis and ER stress, has been actively studied in the recent decades, the subject of this review is highly relevant.

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.04.005
      Issue No: Vol. 48 (2018)
  • Evaluation of changes in the macro and micronutrients homeostasis of
           transgenic and non-transgenic soybean plants after cultivation with silver
           nanoparticles through ionomic approaches
    • Authors: Rodrigo Moretto Galazzi; Marco Aurélio Zezzi Arruda
      Pages: 181 - 187
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Rodrigo Moretto Galazzi, Marco Aurélio Zezzi Arruda
      An ionomic approach is conducted for evaluating the silver distribution, in different soybean plant compartments, as well as its influence on the homeostasis of some macro (P and S) and micro (Cu, Fe, Mn and Zn) nutrients. Silver is added to transgenic and non-transgenic soybean plants as nanoparticles or silver nitrate for comparative purposes. The transgenic plants translocate a higher amount of silver (100 and 65% for silver nanoparticles and silver nitrate expositions, respectively) than non-transgenic, and considering the treatments, the cultivation with silver nanoparticles results in a higher translocation rate (100 and 21% for transgenic and non-transgenic plants, respectively). In addition, significant differences are found (p < 0.05) considering those macro and micronutrients in all plant compartments, mainly in the roots of those treated plants, indicating not only that both silver forms evaluated interfere in the plant metabolism, but also their toxicities.
      Graphical abstract image

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.04.004
      Issue No: Vol. 48 (2018)
  • Influence of F1 hybridization on the metal uptake behaviour of pine trees
           (Pinus nigra x Pinus thunbergiana; Pinus thunbergiana x Pinus nigra)
    • Authors: Iva Juranović-Cindrić; Michaela Zeiner; Ana Starčević; Zlatko Liber; Gordana Rusak; Marilena Idžojtić; Gerhard Stingeder
      Pages: 190 - 195
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Iva Juranović-Cindrić, Michaela Zeiner, Ana Starčević, Zlatko Liber, Gordana Rusak, Marilena Idžojtić, Gerhard Stingeder
      Pine needles have been considered to be useful bio-indicators for air pollution. This phenomenon can be used for environmental studies for monitoring purposes. Additionally, this fact offers the possibility to study uptake and accumulation behaviour not only in different species, but also in hybrids obtained from common pine trees to inheritage processes. Therefore, needles of Pinus nigra Arnold and Pinus thunbergiana Franco as well as of their F1 hybrids were investigated for essential and non-essential metals, such as Al, B, Ba, Ca, Cd, Co, Cu, Cr, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Sr, and Zn. The samples underwent acidic microwave-assisted digestion prior to analysis inductively coupled plasma atomic emission spectrometry (ICP-AES). Furthermore flavonoids were quantitatively determined to prove hybrid character. Regarding all determined analytes, increase and decrease of uptake in the needles of the hybrids were evaluated in comparison to the needles of the parent pine species to see which parent is the dominant one. In the hybrids higher amounts of Al, Cd, Mo, Ni, Mg, Mn, and Zn were found. Different behaviour was registered for flavonoids than for metals, due to different metabolic pathways.
      Graphical abstract image

      PubDate: 2018-04-18T10:13:11Z
      DOI: 10.1016/j.jtemb.2018.04.009
      Issue No: Vol. 48 (2018)
  • Association between cadmium and androgen receptor protein expression
           differs in prostate tumors of African American and European American men
    • Authors: Christine M. Neslund-Dudas; Russell B. McBride; Ashoka Kandegedara; Benjamin A. Rybicki; Oleksandr N. Kryvenko; Dhananjay Chitale; Nilesh Gupta; Sean R. Williamson; Craig G. Rogers; Carlos Cordon-Cardo; Andrew G. Rundle; Albert M. Levin; Q. Ping Dou; Bharati Mitra
      Pages: 233 - 238
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Christine M. Neslund-Dudas, Russell B. McBride, Ashoka Kandegedara, Benjamin A. Rybicki, Oleksandr N. Kryvenko, Dhananjay Chitale, Nilesh Gupta, Sean R. Williamson, Craig G. Rogers, Carlos Cordon-Cardo, Andrew G. Rundle, Albert M. Levin, Q. Ping Dou, Bharati Mitra
      Cadmium is a known carcinogen that has been implicated in prostate cancer, but how it affects prostate carcinogenesis in humans remains unclear. Evidence from basic science suggests that cadmium can bind to the androgen receptor causing endocrine disruption. The androgen receptor is required for normal prostate development and is the key driver of prostate cancer progression. In this study, we examined the association between cadmium content and androgen receptor protein expression in prostate cancer tissue of African American (N = 22) and European American (N = 30) men. Although neither overall tumor cadmium content (log transformed) nor androgen receptor protein expression level differed by race, we observed a race-cadmium interaction with regard to androgen receptor expression (P = 0.003) even after accounting for age at prostatectomy, smoking history, and Gleason score. African American men had a significant positive correlation between tumor tissue cadmium content and androgen receptor expression (Pearson correlation = 0.52, P = 0.013), while European Americans showed a non-significant negative correlation between the two (Pearson correlation = −0.19, P = 0.31). These results were unchanged after further accounting for tissue zinc content or dietary zinc or selenium intake. African American cases with high-cadmium content (>median) in tumor tissue had more than double the androgen receptor expression (0.021 vs. 0.008, P = 0.014) of African American men with low-cadmium level. No difference in androgen receptor expression was observed in European Americans by cadmium level (high 0.015 vs. low 0.011, P = 0.30). Larger studies are needed to confirm these results and if upheld, determine the biologic mechanism by which cadmium increases androgen receptor protein expression in a race-dependent manner. Our results suggest that cadmium may play a role in race disparities observed in prostate cancer.

      PubDate: 2018-04-25T04:39:35Z
      DOI: 10.1016/j.jtemb.2018.04.006
      Issue No: Vol. 48 (2018)
  • The toxic effect of gallic acid on biochemical factors, viability and
           proliferation of rat bone marrow mesenchymal stem cells was compensated by
           boric acid
    • Authors: Mohammad Hussein Abnosi; Somayeh Yari
      Pages: 246 - 253
      Abstract: Publication date: July 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 48
      Author(s): Mohammad Hussein Abnosi, Somayeh Yari
      Objective Gallic acid (GA) and boron are found in many plants. Our previous studies showed 6 ng/ml boric acid (BA) had positive effect on biochemistry of rat bone marrow mesenchymal stem cells (MSCs) and their osteogenic differentiation. Therefore, we investigate the effect of different doses of GA alone and in the presence of BA on MSCs. Materials and methods the viability of MSCs was assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue at 12, 24 and 36 h in presence of different concentration of GA. Then 30 and 120 μM of GA as well as 6 ng/ml of BA in 36 h were selected for further study. The proliferation, Morphology, sodium and potassium level, concentration of calcium, activity of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) as well as malondialdehyde (MDA) concentration, total antioxidant capacity (FRAP) and activity of superoxide dismutase (SOD) and catalase (CAT) were estimated. Result Results showed GA alone reduced viability, proliferation, nuclear diameter and cytoplasm area. In addition, GA showed anaerobic metabolic shift but no change in MDA and scavenging enzymes. Both concentration of GA caused elevation of FRAP, whereas only at 120 μM increased the sodium-potassium and reduced calcium. The co-treatment of GA and BA improves the viability, proliferation and morphology of the cells. In addition, co-treatment compensated the metabolic shift caused by GA and could balance the potassium level and FRAP as it was raised by GA. Conclusion Although GA content of tea is harmful to the cells but simultaneous consumption of fruits and vegetables as a rich source of boron might compensate the damaging effect of GA.

      PubDate: 2018-06-01T01:07:10Z
      DOI: 10.1016/j.jtemb.2018.04.016
      Issue No: Vol. 48 (2018)
  • Effect of manganese on neural endocrine hormones in serum of welders and
    • Authors: Shi-Yan Hai-Lan; Luo Richard Mailman Zhao-Cong Yu-Wen Zhang Mei Cai
      Abstract: Publication date: December 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 50
      Author(s): Shi-Yan Ou, Hai-Lan Luo, Richard B. Mailman, Zhao-Cong Li, Yu-Wen Zhang, Mei Cai, Xiao-Wei Huang, Shao-Jun Li, Yue-Ming Jiang
      Background Although manganese (Mn)-induced neurotoxicity effects are well known among occupational Mn exposure, few reports have investigated the effects on endocrine systems among welders and smelters. Objective To determine the effect of high level occupational manganese (Mn) exposure on neuropsychological parameters and hormonal status. Methods We used a cross-sectional design with 52 welders, 48 smelters and 43 age-matched office workers from the same factory in China. We analyzed serum endocrine hormones level and airborne Mn concentrations. Erythrocyte and urine Mn levels were quantified using inductively-coupled plasma atomic emission spectroscopy. Results The geometric mean of air Mn concentrations for the welders and smelters were 19.7 and 273.1 μg/m3, respectively. Mn concentrations in erythrocytes of smelters were markedly greater than those in controls and welders, but there was no difference between the erythrocytes Mn levels of Control and welders. We also found an increase of Mn levels in the urine of both welders and smelters vs. controls; Mn levels in urine of smelters were higher than in welders. Self-reported neurobehavioral symptoms were higher in welders and smelters than in controls. Finally, thyroid-stimulating hormone (TSH) levels of welders were significantly lower than in controls, whereas smelters had lower prolactin (PRL), testosterone (TST) and follicle-stimulating hormone (FSH) concentrations than either controls or welders. Conclusions These results show that smelters have higher Mn exposure than do welders, and that Mn levels in erythrocytes or urine can be a marker for exposure. Moreover, high level occupational Mn exposure increases adverse neurobehavioral effects, and also may disrupt endocrine systems.
      Graphical abstract image

      PubDate: 2018-06-01T01:07:10Z
  • Analysis of aluminum, minerals and trace elements in the milk samples from
           lactating mothers in Hamadan, Iran
    • Authors: Masoumeh Taravati; Javad Aliasghar Vahidinia Fateme Samiee Jomana Elaridi Mostafa
      Abstract: Publication date: December 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 50
      Author(s): Masoumeh Taravati Javad, Aliasghar Vahidinia, Fateme Samiee, Jomana Elaridi, Mostafa Leili, Javad Faradmal, Alireza Rahmani
      The present cross-sectional study is aimed at analyzing the breast milk of lactating mothers in Hamadan, Iran for aluminum and several minerals and trace elements. Ten governmental health care centers were utilized to facilitate collection of breast milk samples. The breast milk samples were collected at 1, 2, 6, 7, and 12 months postpartum from one hundred healthy lactating women, who delivered full-term newborns. Detection of sodium (Na), zinc (Zn), calcium (Ca), iron (Fe), copper (Cu), magnesium (Mg) and aluminum (Al) levels was conducted with the use of Inductively Coupled Plasma Mass Spectrometry (ICP-MS). This method has shown high accuracy, precision, sensitivity, and linearity for the wide range of concentrations. The accumulated data were not normally distributed; thus, the non-parametric Mann-Whitney U test was used in the statistical analysis of the results. Mean concentrations of Fe, Zn, Cu, Ca, Mg, and Na were 0.75, 1.38, 0.35, 255, 34.58, and 155.72 μg/mL, respectively. The mean level of Al, a well-known neurotoxic metal, was determined to be an alarming 0.191 μg/mL. Moreover, 95% of participants contained very harmful concentrations of Al in their milk. This study also revealed Zn deficiency in about 50% of milk samples. Further investigation is needed to elucidate sources of exposure and factors that may influence maternal and fetal exposure to aluminum.

      PubDate: 2018-06-01T01:07:10Z
  • Low-dose Thimerosal (ethyl-mercury) is still used in infants` vaccines:
           Should we be concerned with this form of exposure'
    • Abstract: Publication date: September 2018
      Source:Journal of Trace Elements in Medicine and Biology, Volume 49
      Author(s): José G. Dórea
      In developing countries, Thimerosal-containing vaccines (TCV) are the main causes of organic Hg exposure for newborns, neonates, and infants immunized with TCV. This article addresses early-life exposure to this unique organic mercury compound (ethylmercury-EtHg) and the risks of its exposure. English language studies pertaining to Thimerosal/EtHg toxicity and exposure during early life were searched in PubMed; and, those publications judged to be relevant to the topic of this review were selected. The risk from the neurotoxic effects of pre- and post-natal Hg exposures depend, in part, on aggravating or attenuating environmental and/or genetic-associated factors. Health authorities in charge of controlling infectious disease dismiss the toxicology of mercury (immunological and subtle neurological effects as insignificant) related to low-dose Thimerosal. The review addresses the evidence that brings into question the safety of Thimerosal that is still present in vaccines given to pregnant women, infants, and children in developing countries, and recognizes the ethical imperative to extend the use of Thimerosal-free vaccines to developing countries, not just developed countries.

      PubDate: 2018-06-01T01:07:10Z
  • Lithium chloride attenuates mitomycin C induced necrotic cell death in
           MDA-MB-231 breast cancer cells via HMGB1 and Bax signaling
    • Authors: Mahdieh Razmi; Azra Rabbani-Chadegani; Fatemeh Hashemi-Niasari; Parinaz Ghadam
      Abstract: Publication date: Available online 15 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Mahdieh Razmi, Azra Rabbani-Chadegani, Fatemeh Hashemi-Niasari, Parinaz Ghadam
      The clinical use of potent anticancer drug mitomycin C (MMC) has limited due to side effects and resistance of cancer cells. The aim of this study was to investigate whether lithium chloride (LiCl), as a mood stabilizer, can affect the sensitivity of MDA-MB-231 breast cancer cells to mitomycin C. The cells were exposed to various concentrations of mitomycin C alone and combined with LiCl and the viability determined by trypan blue and MTT assays. Proteins were analyzed by western blot and mRNA expression of HMGB1 MMP9 and Bcl-2 were analyzed by RT-PCR. Flow cytometry was used to determine the cell cycle arrest and percent of apoptotic and necrotic cells. Concentration of Bax assessed by ELISA. Exposure of the cells to mitomycin C revealed IC50 value of 20 μM, whereas pretreatment of the cells with LiCl induced synergistic cytotoxicity and IC50 value declined to 5 μM. LiCl combined with mitomycin C significantly down-regulated HMGB1, MMP9 and Bcl-2 gene expression but significantly increased the level of Bax protein. In addition, the content of HMGB1 in the nuclei decreased and pretreatment with LiCl reduced the content of HMGB1 release induced by MMC. LiCl increased mitomycin C-induced cell shrinkage and PARP fragmentation suggesting induction of apoptosis in these cells. LiCl prevented mitomycin C-induced necrosis and changed the cell death arrest at G2/M-phase. Taking all together, it is suggested that LiCl efficiently enhances mitomycin C-induced apoptosis and HMGB1, Bax and Bcl-2 expression may play a major role in this process, the findings that provide a new therapeutic strategy for LiCl in combination with mitomycin C.

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.03.011
  • School meals’ centesimal and mineral composition and their nutritional
           value for Brazilian children
    • Authors: Fabiana C.O. França; Iziane S. Andrade; Mariangela V. Lopes Silva; Maurício S. Lordêlo; Renata G. Costa; José A. Menezes-Filho
      Abstract: Publication date: Available online 15 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Fabiana C.O. França, Iziane S. Andrade, Mariangela V. Lopes Silva, Maurício S. Lordêlo, Renata G. Costa, José A. Menezes-Filho
      The assessment of meals served under the Brazilian National School Meal Program (PNAE) is an important tool to verify its adequacy to the proposed parameters and the nutritional needs of school-aged children. The aim of this study was to evaluate the centesimal and mineral composition of the meals offered by the program and adequacy to the nutritional recommendations in three municipalities of the state of Bahia, Brazil. Centesimal composition of meals was determined according to the reference guidelines and mineral composition was analyzed by atomic absorption spectrometry. Non-parametric analysis of variance was used to test the differences of the medians among the municipalities and Student-t test to compare the means between the two sampling periods. There were inadequacies in the carbohydrate, lipid and protein contents, and none of the municipalities reached the recommendation of 20%. Mineral concentration, especially Fe, Se, Cu and K were much lower than expected. Sodium levels were three-folds higher than the recommended, being worrisome due to higher risk of elevated blood pressure. There were inadequacies with regard to the PNAE guidelines, and there is a need to reevaluate the meals that are being offered to better meet childreńs needs and to form healthy habits from childhood.
      Graphical abstract image

      PubDate: 2018-03-19T04:41:21Z
      DOI: 10.1016/j.jtemb.2018.03.013
  • Optimal dosage and early intervention of L-ascorbic acid inhibiting
           K2Cr2O7-induced renal tubular cell damage
    • Authors: Tzeng-Jih Lin; Yeou-Lih Huang; Jung-San Chang; Kuan-Ting Liu; Meng-Chi Yen; Fen-Wei Chen; Yueh-Lun Shih; Jo-Chi Jao; Po-Chi Huang; I-Jeng Yeh
      Abstract: Publication date: Available online 2 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Tzeng-Jih Lin, Yeou-Lih Huang, Jung-San Chang, Kuan-Ting Liu, Meng-Chi Yen, Fen-Wei Chen, Yueh-Lun Shih, Jo-Chi Jao, Po-Chi Huang, I-Jeng Yeh
      Chromium poisoning can cause renal failure and death. Chromium intoxication may be managed using L-ascorbic acid (vitamin C) therapy. However, the evidence supporting the effectiveness of this treatment is insufficient, and the mechanism of action has not been clarified in renal cells. In this study, our results showed that the optimal regimen of L-ascorbic acid therapy in human epithelial renal proximal tubule cells, HK-2 cells, was 30 μg/mL. Supplementation of L-ascorbic acid with 30 μg/mL and within 8 hours of chromium intoxication (K2Cr2O7, Cr6+) was effective to inhibit renal tubular cell damage by blocking generation of free radicals, cell apoptosis, and autophagy. Intracellular chromium concentrations were estimated using electrothermal atomic absorption spectrometry. Treatment of L-ascorbic acid within 8 hours of chromium intoxication significantly decreased the entry of chromium into the cells. Moreover, concomitant administration of L-ascorbic acid with repeatedly dosing at 8-hourly intervals had a better protective effect at lower concentration of L-ascorbic acid when compared to single dosing of L-ascorbic acid at an early time point of chromium intoxication. These findings might help physicians develop effective therapy strategies in renal failure.
      Graphical abstract image

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.022
  • Mössbauer spectroscopic study of transformations of iron species by the
           cyanobacterium Arthrospira platensis (formerly Spirulina platensis)
    • Authors: Yurii D. Perfiliev; Alexandr Kh. Tambiev; Maxim A. Konnychev; Anatoly V. Skalny; Elena S. Lobakova; Mikhail P. Kirpichnikov
      Abstract: Publication date: Available online 2 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Yurii D. Perfiliev, Alexandr Kh. Tambiev, Maxim A. Konnychev, Anatoly V. Skalny, Elena S. Lobakova, Mikhail P. Kirpichnikov
      In the present paper, Mössbauer spectroscopic studies of dry biomass samples of the cyanobacterium Arthrospira platensis (formerly known as Spirulina platensis) were performed with regard to metabolic iron accumulation. 57Fe Mössbauer parameters of iron in the biomass correspond to ferrihydrite. Spectra of iron hydroxides in A. platensis biomass differ from those of iron complexes with ethylenediaminetetraacetic acid injected to Zarrouk culture medium. The limit of saturation of A. platensis trichomes with iron in the form of ferrihydrite was found to be 5 μg/ml (0.09 μmol/ml) Fe in the culture medium. Conglomerates precipitated in the medium at higher iron concentrations also contain ferrihydrite but the ratio of the crystal lattice forms is different from that in the biomass.

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.030
  • Anti-inflammatory and antinociceptive effects of 2,2`-dipyridyl diselenide
           through reduction of inducible nitric oxide synthase, nuclear factor-kappa
           B and c-Jun N-terminal kinase phosphorylation levels in the mouse spinal
    • Authors: Suzan Gonçalves Rosa; César Augusto Brüning; Ana Paula Pesarico; Ana Cristina Guerra de Souza; Cristina Wayne Nogueira
      Abstract: Publication date: Available online 1 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Suzan Gonçalves Rosa, César Augusto Brüning, Ana Paula Pesarico, Ana Cristina Guerra de Souza, Cristina Wayne Nogueira
      Appropriate treatment of pain requires analgesics and anti-inflammatory drugs generally associated with undesirable side effects and not fully effective in a significant proportion of patients. Organoselenium compounds elicit a plenty of pharmacological effects in different animal models. Among these compounds, the 2,2`-dipyridyl diselenide (DPD) has a potent antioxidant effect and low toxicity. In this way, the aim of this study was to investigate the possible DPD antinociceptive effect and its mechanism of action, as well as the safety of the compound. Female Swiss mice were treated with vehicle or DPD (0.01–50 mg/kg) intragastrically. Dose–response curve and time-course of the antinociceptive effect of DPD were performed on formalin and tail immersion tests. Morphine (2.5 mg/kg, subcutaneous, 15 min earlier) was used as a positive control in behavioral tests. The results showed that DPD presents a rapid antinociceptive effect in low doses, without changing the spontaneous locomotor activity and parameters of toxicity in mice. The DPD antinociceptive effect was also confirmed in male Swiss mice in both formalin and tail immersion tests. In addition, DPD reduced the paw edema induced by 2.5% formalin and ear edema induced by 2.5% croton oil. L-arginine (600 mg/kg, intraperitoneally) reduced the DPD antinociceptive effect in the first phase of the formalin test. Moreover, DPD attenuated the increase in iNOS, NF-κB and JNK phosphorylation in the spinal cord of mice injected with formalin. These results showed that DPD exerts peripheral and central nociceptive actions associated with anti-inflammatory effect and this organoselenium compound could be an interesting alternative therapy for pain treatment.

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.021
  • Total content and in vitro bioaccessibility of Tellurium in Brazil nuts
    • Authors: Otávio Berenguel; Gustavo de S. Pessôa; Marco A.Z. Arruda
      Abstract: Publication date: Available online 1 March 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Otávio Berenguel, Gustavo de S. Pessôa, Marco A.Z. Arruda
      Alongside the Brazil nut’s role as an excellent source of vitamins, oil, fatty acids, lipids and nutrients, it is also recognized as a rich source of selenium. The pathway along which selenium and sulfur are metabolized in plants is theorized to be the same as that used for tellurium. Total tellurium content and its bioaccessibility are then evaluated by ICP-MS. Interferences and sample preparation are evaluated for the accurate determination of tellurium, and the accuracy determined through analysis of the certified reference material 1643e. A concentration of 4.02 ± 0.391 ng g−1 is obtained as an average concentration through external and internal calibrations. Through this reliable result, tellurium bioaccessibility in Brazil nuts is obtained via an in vitro validated unified bioaccessibility method. Values of 32% and 30% of total tellurium are available in the gastric and gastrointestinal fractions, respectively.
      Graphical abstract image

      PubDate: 2018-03-07T03:59:12Z
      DOI: 10.1016/j.jtemb.2018.02.026
  • Assessment of metals bioaccumulation and bioavailability in mussels
           Mytilus galloprovincialis exposed to outfalls pollution in coastal areas
           of Casablanca
    • Authors: Zineb Mejdoub; Younes Zaid; Hmimid Fouzia; Mostafa Kabine
      Abstract: Publication date: Available online 21 February 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Zineb Mejdoub, Younes Zaid, Hmimid Fouzia, Mostafa Kabine
      The present work aims to study the metallic contamination of four sampling sites located nearby major sewage outfalls of the Casablanca coast (Morocco), using indigenous mussels Mytilus galloprovincialis as bioindicators of pollution. This research offered the opportunity to study trace metals bioaccumulation mechanisms, which represent a major factor in assessment processes of the pollution effects in coastal ecosystem health. The bioavailability and the bioaccumulation of trace metals (Cu, Zn, Ni, Pb) were evaluated in order to compare the metallic contamination in mussels’ tissues and find a possible correlation with physiological parameters of this filter feeding species. Our results showed a significant spatiotemporal variation of bioaccumulation, compared to control. A significant correlation coefficient between metals (Zn and Pb) bioavailability and physiological index (CI) was revealed in mussels from the most polluted location. The seasonal variation of trace metal accumulation was also raised; the highest values recorded during the dry period.

      PubDate: 2018-02-26T03:09:08Z
      DOI: 10.1016/j.jtemb.2018.02.018
  • Blood Lead and Cadmium in Age Related Macular Degeneration in a Turkish
           Urban Population
    • Authors: Elif Damar Güngör; Fatma Yülek; Utku Serkant; Yasin Toklu; Asım Hocaoğlu; Şaban Şimsek
      Abstract: Publication date: Available online 21 February 2018
      Source:Journal of Trace Elements in Medicine and Biology
      Author(s): Elif Damar Güngör, Fatma Yülek, Utku Serkant, Yasin Toklu, Asım Hocaoğlu, Şaban Şimsek
      Purpose To evaluate the blood lead (Pb) and cadmium (Cd) levels in age related macular degeneration (AMD) in a turkish urban population. Methods Blood Pb and Cd levels of 31 AMD patients and 24 age and gender matched controls with no sign of AMD were measured using dual atomic absorption spectrophotometer system (AAS). History of hypertension, diabetes mellitus, cigarette smoking, myocardial infarction and stroke were obtained from all subjects. Degree of AMD was grade 4 according to the Age-Related Eye Disease Study grading system. Median blood Pb and Cd levels were compared by using Students’ t test. Results Demographic properties like smoking status, presence of diabetes mellitus or hypertension, cerebrovascular occlusion history, serum cholesterol and lipid levels were not significantly different between groups except history of ischemic heart disease (3.22% vs 25% in AMD and control groups respectively, p = 0.022). Overall in AMD group blood Pb level was 2.83 ± 0.15 μg/l and it was 2.63 ± 0.23 μg/l in control group (p = 0.36). The Cd level was 3.25 ± 0.20 μg/l in AMD group and 3.11 ± 0.25 μg/l in control group (p = 0.67). The mean Pb (2.38 ± 0.88 μg/l vs 2.91 ± 1.37 μg/l for AMD vs control, p = 0.61) and Cd levels (3.06 ± 1.34 μg/l vs 3.35 ± 1.26 μg/l for AMD vs control, p = 0.56) in current and previous smokers with AMD were not significantly different from those of the current and previous smokers in control group. Conclusion Blood Pb and Cd levels which reflect short term exposure were not significantly different in AMD patients and the control group. The difference was not significant either after involvement of previous or current smoker subjects.

      PubDate: 2018-02-26T03:09:08Z
      DOI: 10.1016/j.jtemb.2018.02.019
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