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BIOTECHNOLOGY (227 journals)                  1 2 | Last

Showing 1 - 200 of 227 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 7)
Advances in Bioscience and Biotechnology     Open Access   (Followers: 14)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 7)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 8)
American Journal of Polymer Science     Open Access   (Followers: 29)
Animal Biotechnology     Hybrid Journal   (Followers: 9)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 62)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 5)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 2)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 8)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 1)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 5)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 5)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 6)
Biomédica     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 7)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Bioprinting     Hybrid Journal  
Bioresource Technology Reports     Hybrid Journal  
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 22)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 6)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 5)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 161)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 14)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 7)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 15)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 33)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 2)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Biotribology     Hybrid Journal  
BMC Biotechnology     Open Access   (Followers: 15)
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 3)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 3)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 14)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 9)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access   (Followers: 1)
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 12)
Food Science and Biotechnology     Hybrid Journal   (Followers: 9)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 1)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 1)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 15)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 2)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 6)
Journal of Applied Biomedicine     Open Access   (Followers: 3)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 10)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 7)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 3)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 14)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 2)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 6)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 5)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 16)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 3)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 520)
Network Modeling and Analysis in Health Informatics and Bioinformatics     Hybrid Journal   (Followers: 3)
New Biotechnology     Hybrid Journal   (Followers: 4)
Nigerian Journal of Biotechnology     Open Access  
Nova Biotechnologica et Chimica     Open Access  
NPG Asia Materials     Open Access  
npj Biofilms and Microbiomes     Open Access  
OA Biotechnology     Open Access  
Plant Biotechnology Journal     Open Access   (Followers: 10)
Plant Biotechnology Reports     Hybrid Journal   (Followers: 4)
Preparative Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)

        1 2 | Last

Journal Cover Bioprinting
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   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Online) 2405-8866
   Published by Elsevier Homepage  [3177 journals]
  • A Perspective on the Physical, Mechanical and Biological Specifications of
           Bioinks and the Development of Functional Tissues in 3D Bioprinting
    • Authors: David Williams; Patrick Thayer; Hector Martinez; Erik Gatenholm; Ali Khademhosseini
      Abstract: Publication date: Available online 10 March 2018
      Author(s): David Williams, Patrick Thayer, Hector Martinez, Erik Gatenholm, Ali Khademhosseini
      Bioinks are the basic building blocks for the fabrication of 3D bioprinted constructs. These bioinks play key roles in structural support, adhesion, and differentiation of incorporated cells. Due to diversity of tissue types found in the body, and the need to recapitulate many of the structural and functional characteristics of a target tissue, there are many potential bioinks, but relatively few at the moment possess optimal properties. Bioinks may be classified on the basis on their ultimate role in a 3D bioprinted construct such as imparting biological functionality, serving as sacrificial material, or supporting and providing rigidity to complex constructs. Physical, mechanical and biological characteristics must all be taken into account in the design and composition of bioinks. The incorporation of guidance cues such as topography, growth factors, and morphogens is necessary to control cell fate. These 3D bioprinted constructs should be integrated into bioreactor systems, to support tissue development. Finally, the methodologies to non-destructively monitor both deposited bioinks and construct maturation are necessary for the successful development of a functional tissue.

      PubDate: 2018-03-10T10:06:28Z
      DOI: 10.1016/j.bprint.2018.02.003
  • Laser-based fabrication of 3D hydrogel constructs using bessel beams
    • Authors: Wen Loong Andy Liew; Yilei Zhang
      Abstract: Publication date: Available online 6 March 2018
      Author(s): Wen Loong Andy Liew, Yilei Zhang
      Bioprinting has shown great promise for applications in the field of tissue engineering. However, the printing techniques existing today suffer from several drawbacks including long processing time, poor printing fidelity for high aspect ratio constructs, and poor cytocompatibility. In this report, we describe a laser-based bioprinting technique and demonstrate its ability to address these drawbacks. Unlike commercial Stereolithography systems, the laser used in our proposed technique is in the form of a Bessel Beam (BB) which possesses unique characteristics suitable for bioprinting applications. We demonstrate the successful fabrication of hydrogel fibers with controlled diameter, along with the encapsulation of highly viable cells. We also demonstrate the high throughput fabrication of cell-laden tubular constructs with high fidelity and customizable cross-sections. Finally, 3D hydrogel scaffolds made of both synthetic and natural polymers were fabricated. We believe that our proposed technique could find significant applications in the field of tissue engineering for regenerative medicine and also for in vitro tissue studies.

      PubDate: 2018-03-10T10:06:28Z
      DOI: 10.1016/j.bprint.2018.02.004
  • A review on design for bioprinting
    • Authors: Ibrahim Ozbolat; Hemanth Gudapati
      Pages: 1 - 14
      Abstract: Publication date: September–December 2016
      Source:Bioprinting, Volumes 3–4
      Author(s): Ibrahim Ozbolat, Hemanth Gudapati
      In order to bioprint living tissue and organ constructs, patient-specific anatomical models need to be acquired; however, these models mainly provide external surface information only. The internal architecture of tissue constructs plays a crucial role as it provides a porous environment for media exchange, vascularization, tissue growth and engraftment. This review presents design requirements for bioprinting and discusses currently available medical imaging techniques used in acquisition of anatomical models including magnetic resonance imaging (MRI) and computed tomography (CT), and compares their strengths and limitations. Then, consideration for design architecture is discussed and various approaches in blueprint modeling of tissue constructs are presented for creation of porous architectures. Next, existing toolpath planning approaches for bioprinting of tissues and organs are presented. Design limitations for bioprinting are discussed and future perspectives are provided to the reader.

      PubDate: 2017-09-25T09:45:30Z
      DOI: 10.1016/j.bprint.2016.11.001
      Issue No: Vol. 3-4 (2017)
  • Building the basis for patient-specific meniscal scaffolds: From human
           knee MRI to fabrication of 3D printed scaffolds
    • Authors: I.F. Cengiz; M. Pitikakis; L. Cesario; P. Parascandolo; L. Vosilla; G. Viano; J.M. Oliveira; R.L. Reis
      Pages: 1 - 10
      Abstract: Publication date: March–June 2016
      Source:Bioprinting, Volumes 1–2
      Author(s): I.F. Cengiz, M. Pitikakis, L. Cesario, P. Parascandolo, L. Vosilla, G. Viano, J.M. Oliveira, R.L. Reis
      The current strategies for the transplantation of meniscus should be strengthened to tackle the faced limitations of current methods in the clinics. One of the limitations is that current implants are not patient-specific. There is, therefore, a pressing need in the clinics to develop patient-specific implants. The aim of this study was to demonstrate a semi-automatic way of segmenting meniscus tissues from patients' volumetric knee magnetic resonance imaging (MRI) datasets in order to obtain patient-specific 3D models for 3D printing of patient-specific constructs. High-quality MRI volumetric images were acquired from five healthy male human subjects. The advanced segmentation software, RheumaSCORE, was used for semi-automatic MRI image segmentation of the meniscus tissues. Our methodology allows a full 3D segmentation of the menisci with only minimal interaction on 2D slices. The obtained 3D models were used for the fabrication of tissue engineering scaffolds from polycaprolactone with different internal architectures. The fabricated scaffolds were characterized by micro-computed tomography (µ-CT), scanning electron microscopy (SEM), and mechanical testing. This study demonstrated the 3D fabrication of patient-specific scaffolds with a 3D printer using the reconstructed 3D models obtained by an advanced segmentation method of menisci from knee MRI. This is a step towards a personalized tissue engineering therapy model for the knee meniscus.

      PubDate: 2017-09-25T09:45:30Z
      DOI: 10.1016/j.bprint.2016.05.001
      Issue No: Vol. 1-2 (2017)
  • 3D bioprinting – An Ethical, Legal and Social Aspects (ELSA)
    • Authors: S. Vijayavenkataraman; W.F. Lu; J.Y.H. Fuh
      Pages: 11 - 21
      Abstract: Publication date: March–June 2016
      Source:Bioprinting, Volumes 1–2
      Author(s): S. Vijayavenkataraman, W.F. Lu, J.Y.H. Fuh
      3D printing is one of the most innovative technologies in the current era, while 3D bioprinting is revolutionizing the medical technology industry. Bioprinting technology could help overcome the limitations of the current tissue engineering methods, including the problem of longer waiting times for treatment (especially with organ transplants). While fighting infectious diseases had been the main focus of medicine in the past, dealing with the consequences of a predominantly ageing population will be the priority in the future and bioprinting is a promising technology to tackle this challenge effectively. Bioprinting will not only cater the needs of ageing population but also in the field of paediatrics, where the bioprinted tissue or organ should possess the capability to grow with the patient. As researchers around the world are working on 3D bioprinting of tissues and organs, companies are burgeoning all over, making and marketing new bioprinters. While the research and commercialization are moving at such a rapid pace, the issues surrounding the technology, in terms of ethics, policies, regulations and social acceptance, are not addressed in commensurate. Identifying the ELSA (Ethical Legal and Social Aspects) concerns of this technology at an early stage is not only part of our social responsibility but also in the interest of the future of the technology itself. This paper reviews and foresees these challenges with pragmatism, thereby creating awareness to the researchers and policy makers and to urge a positive course of action in the foreseeable future. The significance of this work will be to address a broad audience, associated with this technology, from scientists to businessmen, engineers to clinicians, laymen to lawmakers. A ‘complete’ policy approach for this technology is recommended rather than a ‘piecemeal’ approach of the various constituents of this technology. An effective course of action will be to setup a multi-disciplinary international panel to work on the policy framework, which will look in to both ‘hard’ and ‘soft’ impacts of 3D bioprinting, the associated ethical challenges, legal measures including patenting and effective controls to prevent the misuse, as well as the social aspects encompassing the cultural and religious differences which accounts for the success of this technology. Setting up national level panels to assess the risk-benefit analysis, taking into consideration the cultural and religious view of its population and other legal and social aspects, might be a good starting point.

      PubDate: 2017-09-25T09:45:30Z
      DOI: 10.1016/j.bprint.2016.08.001
      Issue No: Vol. 1-2 (2017)
  • 3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for
           patient-specific auricular cartilage regeneration
    • Authors: Héctor Martínez Ávila; Silke Schwarz; Nicole Rotter; Paul Gatenholm
      Pages: 22 - 35
      Abstract: Publication date: March–June 2016
      Source:Bioprinting, Volumes 1–2
      Author(s): Héctor Martínez Ávila, Silke Schwarz, Nicole Rotter, Paul Gatenholm
      Auricular cartilage tissue engineering (TE) aims to provide an effective treatment for patients with acquired or congenital auricular defects. Bioprinting has gained attention in several TE strategies for its ability to spatially control the placement of cells, biomaterials and biological molecules. Although considerable advances have been made to bioprint complex 3D tissue analogues, the development of hydrogel bioinks with good printability and bioactive properties must improve in order to advance the translation of 3D bioprinting into the clinic. In this study, the biological functionality of a bioink composed of nanofibrillated cellulose and alginate (NFC-A) is extensively evaluated for auricular cartilage TE. 3D bioprinted auricular constructs laden with human nasal chondrocytes (hNC) are cultured for up to 28 days and the redifferentiation capacity of hNCs in NFC-A is studied on gene expression as well as on protein levels. 3D bioprinting with NFC-A bioink facilitates the biofabrication of cell-laden, patient-specific auricular constructs with an open inner structure, high cell density and homogenous cell distribution. The cell-laden NFC-A constructs exhibit an excellent shape and size stability as well as an increase in cell viability and proliferation during in vitro culture. Furthermore, NFC-A bioink supports the redifferentiation of hNCs and neo-synthesis of cartilage-specific extracellular matrix components. This demonstrated that NFC-A bioink supports redifferentiation of hNCs while offering proper printability in a biologically relevant aqueous 3D environment, making it a promising tool for auricular cartilage TE and many other biomedical applications.
      Graphical abstract image

      PubDate: 2017-09-25T09:45:30Z
      DOI: 10.1016/j.bprint.2016.08.003
      Issue No: Vol. 1-2 (2017)
  • 3-D bioprinting transplantable tissue structures: A perspective for future
           reconstructive surgical transplantation
    • Authors: Daniel J. Thomas
      Pages: 36 - 37
      Abstract: Publication date: March–June 2016
      Source:Bioprinting, Volumes 1–2
      Author(s): Daniel J. Thomas

      PubDate: 2017-09-25T09:45:30Z
      DOI: 10.1016/j.bprint.2016.08.002
      Issue No: Vol. 1-2 (2017)
  • 3D Bioprinting of Liver-mimetic Construct with Alginate/Cellulose
           Nanocrystal Hybrid Bioink
    • Authors: Yun Wu; Zhi Yuan (William) Lin; Andrew C. Wenger; Kam C. Tam; Xiaowu (Shirley) Tang
      Abstract: Publication date: Available online 14 December 2017
      Author(s): Yun Wu, Zhi Yuan (William) Lin, Andrew C. Wenger, Kam C. Tam, Xiaowu (Shirley) Tang
      3D bioprinting is a novel platform for engineering complex, three-dimensional (3D) tissues that mimic real ones. The development of hybrid bioinks is a viable strategy that integrates the desirable properties of the constituents. In this work, we present a hybrid bioink composed of alginate and cellulose nanocrystals (CNCs) and explore its suitability for extrusion-based bioprinting. This bioink possesses excellent shear-thinning property, can be easily extruded through the nozzle, and provides good initial shape fidelity. It has been demonstrated that the viscosities during extrusion were at least two orders of magnitude lower than those at small shear rates, enabling the bioinks to be extruded through the nozzle (100 μm inner diameter) readily without clogging. This bioink was then used to print a liver-mimetic honeycomb 3D structure containing fibroblast and hepatoma cells. The structures were crosslinked with CaCl2 and incubated and cultured for 3 days. It was found that the bioprinting process resulted in minimal cell damage making the alginate/CNC hybrid bioink an attractive bioprinting material.
      Graphical abstract image

      PubDate: 2017-12-17T03:42:50Z
      DOI: 10.1016/j.bprint.2017.12.001
    • Authors: Ashkan Shafiee; Cyrille Norotte; Elham Ghadiri
      Abstract: Publication date: Available online 18 October 2017
      Author(s): Ashkan Shafiee, Cyrille Norotte, Elham Ghadiri
      Bioprinting provides an exceptional platform for tissue engineers to deposit cells onto substrates with precision, and to construct three-dimensional (3D) biological structures such as tissues and organs. In extrusion bioprinting of cells, cellular bioink particles in the form of multicellular systems are printed on supportive materials such as agarose. Post-printing and before implantation, the 3D biological structure undergoes a complex maturation process governed by both biological and physical mechanisms. Controlling the maturation time to expedite the regeneration of human tissues remains challenging. Herein, we provide evidence that the fusion of cellular bioink (a critical step in tissue maturation) can be accelerated by higher apparent tissue surface tension (ATST) of the cellular bioink particles. Two different preparation methods, namely the egg holder aggregate maker and cylindrical aggregate fabrication, were used to fabricate cellular bioink of Chinese hamster ovary (CHO) and human skin fibroblast (HSF) cells. Each method produced distinct ATSTs from aggregates of the same cell type. The characteristic fusion times for CHO aggregates with 14.71 dyne/cm and 22.8 dyne/cm were found 181.95hours and 93.15hours respectively. The same trend was observed for HSF as aggregates with a higher surface tension fused faster. Therefore, controlling tissue fusion can be achieved through adjusting the ATST of bioprinted spheroids via our fabrication method. This is an essential initial step towards engineering tissues of defined anatomical and histological structures.

      PubDate: 2017-10-24T09:08:02Z
      DOI: 10.1016/j.bprint.2017.10.001
  • Parameter optimization for 3D bioprinting of hydrogels
    • Authors: Braeden Webb; Barry Doyle
      Abstract: Publication date: Available online 14 September 2017
      Author(s): Braeden Webb, Barry Doyle
      Successful bioprinting of hydrogels relies on geometric accuracy and cell viability, both of which are influenced by a number of variable printing parameters. Despite much research aimed at the resulting quality of bioprinted structures, there is no standard method of comparing bioprint results. In this study, we have developed a simple method of assessing the bioprint results from a range of printing parameters in a standardized manner applicable to extrusion-based bioinks. The purpose of the parameter optimization index (POI) is to minimize the shear stress acting on the bioink, and thus on the encapsulated cells, while ensuring the maximum geometric accuracy is obtained. Here we demonstrate the use of the POI on a blend of 7% alginate and 8% gelatin, and test the printing achieved through 25, 27, and 30 gauge print nozzles at 1 – 6 mm/s print speeds, and at 100 – 250 kPa print pressures. In total, we tested 72 printing configurations. Our data shows that for this particular hydrogel blend, the optimum print is obtained with a 30 gauge nozzle, 100 kPa print pressure and 4 mm/s print speed. The POI is intuitive and easy to assess, and could be a useful method across a wide range of 3D bioprinting research and development applications.

      PubDate: 2017-09-19T04:28:59Z
      DOI: 10.1016/j.bprint.2017.09.001
  • Biomimetic 3D Printed Scaffolds for Meniscus Tissue Engineering
    • Authors: Alexander Szojka; Karamveer Lalh; Stephen H.J. Andrews; Nadr M. Jomha; Martin Osswald; Adetola B. Adesida
      Abstract: Publication date: Available online 17 August 2017
      Author(s): Alexander Szojka, Karamveer Lalh, Stephen H.J. Andrews, Nadr M. Jomha, Martin Osswald, Adetola B. Adesida
      The menisci distribute loads to protect the articular cartilage of the knee joint from excessive stress. Injuries to their avascular inner regions do not heal, disrupt function, and increase the risk for knee osteoarthritis. Meniscus tissue engineering aims to restore normal meniscus function by use of regenerated tissue on bioengineered scaffolds. The primary purpose of this study was to design and 3D print polycaprolactone scaffolds that recapitulate the shape and structural components of the meniscus extracellular matrix to provide a template and structural support for complete cell-based meniscus regeneration. A secondary aim was to characterize 3D printed polycaprolactone scaffold fibre architectures with varied fibre spacings, offsets between layers, and circumferential orientations in terms of their equilibrium compressive modulus. Meniscus scaffolds were produced layer-by-layer with a biomimetic fibre architecture inspired by the circumferentially- and radially- oriented collagen fibre bundles found in the native tissue. Suture tabs were incorporated to facilitate fixation at the meniscal horns and the coronary attachments. These scaffolds were designed to have 100% pore interconnectivity and 61% porosity. The equilibrium compressive modulus at 10% strain of the meniscus scaffold architecture was 18.8 ± 3.1 MPa (mean ± standard deviation, n=3) and ultimate load in a suture pull-out test on the anterior horn suture tab was 32 N. The scaffolds produced in this study demonstrate a promising strategy for meniscus tissue engineering as they provide a complete biomimetic template for regeneration of functional components of the meniscus extracellular matrix.

      PubDate: 2017-08-21T03:15:06Z
      DOI: 10.1016/j.bprint.2017.08.001
  • Microcontact printing of tissue precursors via geometrically patterned
           shape-changing hydrogel stamps preserves cell viability and organization
    • Authors: Olukemi O. Akintewe; Samuel J. DuPont; Kranthi Kumar Elineni; Michael C. Cross; Ryan G. Toomey; Nathan D. Gallant
      Abstract: Publication date: Available online 3 June 2017
      Author(s): Olukemi O. Akintewe, Samuel J. DuPont, Kranthi Kumar Elineni, Michael C. Cross, Ryan G. Toomey, Nathan D. Gallant
      In vitro fabrication of tissues is sought for developing platforms to screen candidate drugs and study tissue morphogenesis, and ultimately to regenerate damaged organs. In this study, a modified microcontact printing technique with patterned shape-shifting poly(N-isopropylacrylamide) hydrogels was used to print organized 3T3 fibroblast tissue precursors to target surfaces coated with either fibronectin, collagen type 1 or poly-L-lysine. The patterned hydrogels directed cell-organization on the stamp, and a subsequent shape-shift of the hydrogel triggered instantaneous release of the tissue precursor from the stamp. It was observed that post printing, the tissue precursors underwent rapid repolarization with the emergence of focal adhesion complexes on a time-scale much faster compared to cells seeded by standard sedimentation. Moreover, a minimum stamp pressure was necessary for transfer, however, at stamp pressures above approximately 1psi, cell viability was strongly compromised. To demonstrate the ability to print other cell types using this transfer printing technique, skeletal myoblast tissue precursors were also printed. These results suggest that tissue modules can be organized and transferred with intact pre-patterned morphologies from shape-changing hydrogel structures using a microcontact printing technique. This approach may enable the bottom-up construction of high cell density, scaffold-free tissues with programmed configuration down to the individual cell level.

      PubDate: 2017-06-03T15:25:00Z
      DOI: 10.1016/j.bprint.2017.06.001
  • Novel Development of 3D printable UV-curable Silicone for Multimodal
           Imaging Phantom
    • Authors: E. In; E. Walker; H.E Naguib
      Abstract: Publication date: Available online 25 May 2017
      Author(s): E. In, E. Walker, H.E Naguib
      Medical imaging is an effective medical technique used to diagnose the cancer and other diseases. To optimize medical imaging, a calibration medium or phantom with tissue-mimicking properties is required. A phantom is a specially engineered object that is used in calibration or tuning of the medical imaging modalities, specifically Magnetic Resonance Imaging (MRI) and Computed Tomography (CT). Most of the phantoms investigated were fabricated with water-based polymer gels such as carrageenan, agar, and poly(vinyl alcohol). However, due to water content fluctuation, the stability of the relaxation times, T 1 and T 2 , are problematic. Therefore, there is a demand for the development of phantoms fabricated from solid material without stability issues. Furthermore, there are potential applications of phantoms as surgical planning tools for health practitioner training. In this study, we fabricated Ultraviolet (UV)-curable silicone with various concentrations of water and hydrophilic silicone content to investigate its mechanical and imaging properties. The results demonstrate that the silicone cures faster with addition of water within 30seconds to be applicable for 3D printing. Imaging properties for ultrasound, MRI and CT also demonstrated improvement with addition of water content. With optimal properties, silicone can be used to fabricate the phantom using 3D printing technique.

      PubDate: 2017-05-29T11:02:57Z
      DOI: 10.1016/j.bprint.2017.05.003
  • Elucidating role of Silk-gelatin bioink to recapitulate articular
           cartilage differentiation in 3D bioprinted constructs
    • Authors: Shikha Chawla; Aditi Kumar; Prasad Admane; Amitabha Bandyopadhyay; Sourabh Ghosh
      Abstract: Publication date: Available online 17 May 2017
      Author(s): Shikha Chawla, Aditi Kumar, Prasad Admane, Amitabha Bandyopadhyay, Sourabh Ghosh
      Tissue engineered cartilage has never been evaluated with an aim to distinguish between transient and articular cartilage. A major drawback of existing state-of-the art engineered cartilage is cellular hypertrophy, leading to development of transient cartilage which ultimately undergoes endochondral ossification to form bone trabeculae. As a paradigm shift, using 3D bioprinting, we have evaluated six different conditions for best outcome vis-à-vis articular cartilage differentiation as assessed by expression of a constellation of markers (like Autotaxin, lubricin etc). Our study strongly suggests that BMSCs undergo hypertrophic differentiation in the presence of TGF-β1, while in the absence of TGF-β1 BMSCs encapsulated in 3D bioprinted silk-gelatin bioink matrix undergo articular cartilage differentiation. Our study provides novel insights into direct regulatory role of silk-gelatin bioink on IHH and Wnt signaling pathways in controlling hypertrophy during chondrogenic differentiation of BMSCs. 3D bioprinted silk-gelatin constructs enabled adequate cellular attachment, proliferation and most importantly, articular cartilage differentiation. Interestingly, we observed close similarities between the signaling pathways associated with the 3D bioprinted constructs with respect to the signaling pathways with embryonic cartilage development suggesting our engineered cartilage tissue to be a prospective tissue equivalent with potential of providing the essential instructive elements for activating pathways of organogenesis in patient-specific manner.
      Graphical abstract image

      PubDate: 2017-05-19T10:10:52Z
      DOI: 10.1016/j.bprint.2017.05.001
  • Bioprinting microvessels using an inkjet printer
    • Authors: Sarah Hewes; Andrew D. Wong; Peter C. Searson
      Abstract: Publication date: Available online 17 May 2017
      Author(s): Sarah Hewes, Andrew D. Wong, Peter C. Searson
      Bioprinting enables the construction of complex 3D tissues from biomolecules and extracellular matrix materials. The printing of cells with matrix materials is more challenging due to the constraints imposed by the printing process. Here we demonstrate bioprinting of free-standing microvessels with endothelial cells embedded in a fibrin matrix using a single piezoelectric nozzle. Fibrin tubes are produced using an alginate templating method where alginate/fibrinogen droplets containing cells are printed into a cross-linker bath in a circular pattern. Following triggered dissolution of the alginate, the endothelial cells migrate to the scaffold periphery, spread, and form a confluent monolayer on the fibrin matrix within 14 days.
      Graphical abstract image

      PubDate: 2017-05-19T10:10:52Z
      DOI: 10.1016/j.bprint.2017.05.002
  • Osseointegration Assessment of Extrusion Printed Ti6Al4V Scaffold towards
           Accelerated Skeletal Defect Healing via Tissue in-growth
    • Authors: Pavan Kumar Srivas; Kausik Kapat; Prabhash Dadhich; Pallabi Pal; Joy Dutta; Pallab Datta; Santanu Dhara
      Abstract: Publication date: Available online 28 April 2017
      Author(s): Pavan Kumar Srivas, Kausik Kapat, Prabhash Dadhich, Pallabi Pal, Joy Dutta, Pallab Datta, Santanu Dhara
      3D printing is an additive manufacturing technique to produce metallic components with controlled pore size, total porosity and pore-connectivity. Selective laser melting (SLM), the most widely used technique to develop such kind of metallic components for bone graft applications, requires a controlled atmosphere for reactive metal powder processing. In this study, a simple extrusion-based 3D printing technique is developed to produce porous Ti6Al4V scaffold under ambient environmental condition to overcome the existing limitation. 3D printed Ti6Al4V scaffold with pore size ~ 500µm and total porosity ~ 58% was achieved. The scaffold exhibited ~ 13% shrinkage after sintering, resulting in strut diameter ~ 348µm with persistent inter-particle voids. The compressive strength and elastic modulus values are 39.58 ± 4.56MPa and 450 ± 7.21MPa comparable to cancellous bone mechanical properties. In vitro cytocompatibility assessment of scaffold using mesenchymal stem cells revealed extensive cellular coverage on scaffold surface and differentiation towards bone cell lineage. In vivo studies by scaffold implantation in rabbit femur for four weeks and eight weeks exhibited the scaffold's ability to promote osseointegration and tissue integration through bone in-growth evidenced by micro-CT. Therefore, using this simple and cost-effective technique, bone graft substitute scaffolds could be developed as implant for load bearing applications.
      Graphical abstract image

      PubDate: 2017-05-04T13:28:13Z
      DOI: 10.1016/j.bprint.2017.04.002
  • Integrated 3D Printed Scaffolds and Electrical Stimulation for Enhancing
           Primary Human Cardiomyocyte Cultures
    • Authors: Scott D. Adams; Ajay Ashok; Rupinder K. Kanwar; Jagat R. Kanwar; Abbas Z. Kouzani
      Abstract: Publication date: Available online 28 April 2017
      Author(s): Scott D. Adams, Ajay Ashok, Rupinder K. Kanwar, Jagat R. Kanwar, Abbas Z. Kouzani
      3D printing technology is driving innovation in a wide variety of disciplines, and is beginning to make inroads into the fields of medicine and biology. In particular, 3D printing is being increasingly utilized for the design and fabrication of three-dimensional cell culture scaffolds. This technology allows for scaffolds to be produced rapidly while maintaining a great deal of control over the matrix architecture. This paper presents an effective technique for rapidly designing and fabricating scaffolds from silicone rubber and polycaprolactone (PCL), appropriate for primary human cardiomyocyte cell cultures. Additionally, a stimulation device is developed and presented which can provide 6 channels of wirelessly controlled electrical stimulation to the cell culture scaffolds. The design, fabrication, benchtop evaluation, and biological evaluation of the scaffolds and stimulation device for primary human cardiomyocyte cell culture are presented. The results clearly indicate the effectiveness of both the scaffold fabrication technique and the operation of the stimulation device. The silicone rubber scaffold showed significantly lower cell attachment as compared to the PCL scaffold, validating the suitability of PCL as a material to be employed in the synthesis of bioscaffolds, employed in the management of several medical pathologies such as tissue regeneration and wound healing. Additionally, the biocompatible PCL scaffold stimulated with electrical impulse (5V, 2 ms pulses, 1Hz) exhibited higher cell attachment and differentiated actin cytoskeletal structures as compared to the unstimulated scaffold, indicating the potential of this technique in tissue engineering applications.

      PubDate: 2017-05-04T13:28:13Z
      DOI: 10.1016/j.bprint.2017.04.003
  • Simulation of cortico-cancellous bone structure by 3D printing of bilayer
           calcium phosphate-based scaffolds
    • Authors: Thafar Almela; Ian M Brook; Kimia Khoshroo; Morteza Rasoulianboroujeni; Farahnaz Fahimipour; Mohammadreza Tahriri; Erfan Dashtimoghadam; Abdurahman El-Awa; Lobat Tayebi; Keyvan Moharamzadeh
      Abstract: Publication date: Available online 18 April 2017
      Author(s): Thafar Almela, Ian M Brook, Kimia Khoshroo, Morteza Rasoulianboroujeni, Farahnaz Fahimipour, Mohammadreza Tahriri, Erfan Dashtimoghadam, Abdurahman El-Awa, Lobat Tayebi, Keyvan Moharamzadeh
      Traditional methods of fabrication for porous bone scaffolds are unable to accurately mimic the desirable cortico-cancellous morphology and the structure of the bone. In this study, 3D printing of a β-Tricalcium phosphate (TCP)-based paste was used to develop scaffolds simulating the two distinct cortical and cancellous layers of the natural bone. Laser microscope imaging showed that the pore sizes were 242.2±24.3µm and 410.5± 27.9µm for the cortical and cancellous layers, respectively. Micro CT analysis revealed overall porosity and interconnectivity of 61.8±1.4% and 208707.5±52405, respectively. Mechanical properties were within the range of human cancellous bone with 10.0 ± 2.4MPa strength and 55.5 ± 5.7MPa young's modulus. The X-ray diffraction (XRD) analysis showed that the phase composition of the printed scaffolds was almost identical to pure TCP. Scanning electron microscopy (SEM) and cell vitality assessment indicated significant osteoblastic proliferation on the surface of the scaffolds. The gene expression analysis showed an increase in the level of Collagen I (Col I), Osteonectin (ON), Ostocalcin (OC), and Osteopontin (OPN) with a significant increase in OC and OPN at day 10. In conclusion, 3D printing can be used to develop a TCP-based scaffold with controllable and reproducible microstructures and favourable in vitro biological properties with potential to be further developed to be used for clinical bone regeneration.

      PubDate: 2017-04-20T21:12:49Z
      DOI: 10.1016/j.bprint.2017.04.001
  • Photocrosslinking-based bioprinting: Examining crosslinking schemes
    • Authors: Stephanie Knowlton; Bekir Yenilmez; Shivesh Anand; Savas Tasoglu
      Abstract: Publication date: Available online 18 March 2017
      Author(s): Stephanie Knowlton, Bekir Yenilmez, Shivesh Anand, Savas Tasoglu
      Bioprinting has been widely used for rapid fabrication of biomimetic tissues and organs with the goal of closely mimicking biological structures and their functionalities. Photocrosslinking has emerged as a promising strategy to address several challenges in bioprinting since light is minimally invasive and, by spatiotemporally controlling the application of light, it is possible to crosslink a photosensitive cell-laden bioink locally and on demand to encapsulate cells without significantly reducing cell viability. There have been several approaches to photocrosslinking-based bioprinting. Here, we examine various photocrosslinking schemes in order to guide future innovation in this domain, which will ultimately lead to a range of biomedical applications including generating organs for transplantation, creating tissues for pharmaceutical research, and developing disease models.

      PubDate: 2017-04-20T21:12:49Z
      DOI: 10.1016/j.bprint.2017.03.001
  • Bioprinted fibrin-factor XIII-hyaluronate hydrogel scaffolds with
           encapsulated Schwann cells and their in vitro characterization for use in
           nerve regeneration
    • Authors: Stephanie England; Ajay Rajaram; David J. Schreyer; Xiongbiao Chen
      Abstract: Publication date: Available online 9 December 2016
      Author(s): Stephanie England, Ajay Rajaram, David J. Schreyer, Xiongbiao Chen
      The blood clotting protein fibrin contains cell-binding domains, providing potential advantage for the fabrication of tissue repair scaffolds and for live cell encapsulation. However, fabrication of fibrin scaffolds with encapsulated cells using three dimensional (3D) printing has proven challenging due to the mechanical difficulties of fabricating protein hydrogel scaffolds with defined microstructure. For example, extrusion based 3D printing of fibrin is generally unfeasible because of the low viscosity of precursor fibrinogen solution. Here we describe a novel technique for bioprinting of fibrin scaffolds by extruding fibrinogen solution into thrombin solution, utilizing hyaluronic acid (HA) and polyvinyl alcohol (PVA) to increase the viscosities of the fibrinogen and thrombin solutions, respectively. Clotting factor XIII was also included to enhance fibrin crosslinking. This technique produced 3D fibrin-factor XIII-HA hydrogel scaffolds without the use of support structures. Living Schwann cells encapsulated within these scaffolds during fabrication were viable and proliferated in culture. Moreover, extrusion based bioprinting induced longitudinal alignment of fine fibrin fibers, which apparently provided haptotactic cues directing alignment of encapsulated Schwann cells and elongation of dorsal root ganglion neurites along the 3D printed strands. These aligned fibrin-factor XIII-HA scaffolds mimic the natural fibrin clot that forms between injured nerve ends, and the aligned, encapsulated Schwann cells may provide natural guidance of neurite growth. Bioprinted fibrin-factor XIII-HA scaffolds also have the potential to be applied towards regeneration of other aligned tissues.
      Graphical abstract image

      PubDate: 2017-04-20T21:12:49Z
      DOI: 10.1016/j.bprint.2016.12.001
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