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BIOTECHNOLOGY (227 journals)                  1 2 | Last

Showing 1 - 200 of 227 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 7)
Advances in Bioscience and Biotechnology     Open Access   (Followers: 14)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 7)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 8)
American Journal of Polymer Science     Open Access   (Followers: 29)
Animal Biotechnology     Hybrid Journal   (Followers: 9)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 62)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 5)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 2)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 8)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 1)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 5)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 5)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 6)
Biomédica     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 7)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Bioprinting     Hybrid Journal  
Bioresource Technology Reports     Hybrid Journal  
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 22)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 6)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 5)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 161)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 14)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 7)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 15)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 33)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 2)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Biotribology     Hybrid Journal  
BMC Biotechnology     Open Access   (Followers: 15)
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 3)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 3)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 14)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 9)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access   (Followers: 1)
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 12)
Food Science and Biotechnology     Hybrid Journal   (Followers: 9)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 1)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 1)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 15)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 2)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 6)
Journal of Applied Biomedicine     Open Access   (Followers: 3)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 10)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 7)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 3)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 14)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 2)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 6)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 5)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 16)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 3)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 520)
Network Modeling and Analysis in Health Informatics and Bioinformatics     Hybrid Journal   (Followers: 3)
New Biotechnology     Hybrid Journal   (Followers: 4)
Nigerian Journal of Biotechnology     Open Access  
Nova Biotechnologica et Chimica     Open Access  
NPG Asia Materials     Open Access  
npj Biofilms and Microbiomes     Open Access  
OA Biotechnology     Open Access  
Plant Biotechnology Journal     Open Access   (Followers: 10)
Plant Biotechnology Reports     Hybrid Journal   (Followers: 4)
Preparative Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)

        1 2 | Last

Journal Cover Nigerian Journal of Biotechnology
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   ISSN (Print) 0189-1731
   Published by African Journals Online Homepage  [266 journals]
  • The impact of Palm Oil Mill Effluent (POME) application on the rhizosphere
           heterotrophic population, nitrogen fixing bacteria and enzyme activity of
           an agricultural soil

    • Authors: C.C. Onwusi, C.O. Nwuche
      Abstract: Palm Oil Mill Effluent (POME) is considered an efficient soil conditioner due to its high organic contents but the presence of phenol compounds limits their widespread use in agriculture. In the present study, POME was subjected to both aerobic and anaerobic decomposition to reduce its organic strength and degrade its toxic fractions. It was then applied to support Arachis hypogeal grown in a green house. The rhizosphere environment was monitored for changes in total bacterial counts, nitrogen fixing bacteria and enzyme activities. Results show that the bacteria counts were higher (302.0±1.41 × 105 cfu/g) in mesocosms treated with 100% aerobically digested POME but lower (280.0±1.41 × 105 cfu/g) in the batch treated with anaerobically digested POME. Nitrogen fixing bacteria was 106.0±2.83 × 102 cfu/g in mesocosms treated with aerobically fermented POME compared to 64.0±2.83 × 102 cfu/g from the counterpart treated with anaerobic digests. Dehydrogenase activity was highest (52.83±0.01 mg TPFg-124h-1) in soils treated with aerobically digested POME but not higher (48.65±0.03 mg TPFg-124h-1) than the treatments which received the anaerobically modified effluent. Acid phosphatase activity was lower (201.0±0.28 IU PNP/g/ 24h) in the soils treated with aerobically modified POME but higher (213.5±0.42 IU PNP/g/24h) in batches dosed with anaerobically treated effluent. Urease activity increased to peak level (2.437±0.004 Ppm NH +-N /g/24h) in the 4th week but dropped to its final concentration of 1.615±0.001 Ppm NH +-N /g/24h) in soils treated with anaerobically digested POME. Data from mesocosms treated with the anaerobic digests were lower (2.149±0.004 and 1.500±0.014 ppm NH +-N /g/24h respectively). KeyWords: Palm oil mill effluent, phenol, dehydrogenase, phosphatase, urease 
      Issue No: Vol. 34, No. 1
  • Bioremediation of soil contaminated with crude oil using fresh and
           decomposed animal manure

    • Authors: A. A. Soretire, A. A. Oshiobugie, B. M. Thanni, S. A. Balogun, J. M. Ewetola
      Abstract: Vast majority of rural dwellers stands to bear the brunt of oil spillage and soil pollution, hence the need to develop remediation options using materials that are cheap and readily available. A laboratory incubation study was conducted to evaluate the bioremediation potentials of different types of animal manure on soil contaminated with crude oil (Forcados). Treatments consisting of 20 t ha-1 each of poultry dropping (PD), swine manure(SM) and beef cattle manure (BCM) both in fresh (f) and partially decomposed (p) forms were applied to soils contaminated with (5 % w/w) the crude oil. Their effects were evaluated on total hydrocarbon content (THC), microbial respiration (MR) based on amount of CO2 evolved and pH in situ during a period of 8 weeks of remediation. There was an improvement in the degree of remediation offered by the various bio-stimulants over time. At 4 weeks after amendment (WAA) the level of degradation of THC was in the order: BCMf> control > SMf = PDf > PDp > SMp>BCMp with 17.2, 16.4, 9.6, 9.6, 9.2 and 5.6>4 % of THC degraded respectively. At 8 WAA, % degradation was in the order: BCMf >PDp = PDf >SMf > SMp > BCMp > control with 66, 65.2, 65.2, 64.4, 63.6,62.8 and 61.6 % degradation, respectively. Control was the least while BCMf was the most efficient. Microbial respiration consistently reduced with time in un-remediated soils but BCMf enhanced it over time. This further validates BCMf to be the most efficient in terms of soil remediation. It is, therefore, concluded that fresh beef cattle manure applied at 20 t ha-1 could be very efficient in remediating crude oil polluted soil.Key words: Total hydrocarbon content, microbial respiration, remediation, biostimulant.
      Issue No: Vol. 34, No. 1
  • Assessment of microalgae-influenced biodeterioration of concrete

    • Authors: H.O. Stanley, C. D. Onwukwe, S. D. Peesor
      Abstract: The aim of this study was to isolate microalga involved in the biodeterioration of concrete structures. The growth of algae was monitored between day 1 and 18 using a spectrophotometer (Spectronic 721 model) at varying pH (4.2 and 9.4). To identify the microalgae, aliquots of the isolates was placed on microscope slides and observed with the microscope using x40 Objective lens. Results revealed the highest growth to be between days 12 and 15 with 0.6 optical density, while the least growth was shown between 9 to 12 days. Microalgae isolated in the study include; Chlorella spp., Ulothrix spp., Xanthidium spp., Gleocystis spp., Nitzchia spp., Chlorodium spp., Oedogium spp., Synecoccus spp. and Chlorococcum. Chlorella showed the highest frequency of occurrence with 77.5% while Ulothrix spp. and Xanthidium spp. had the least frequency of 12.5% respectively. The presence of these organisms on concrete structure suggests they contribute to its biodeterioration. There is need therefore to prevent colonization of structures by coating concrete surfaces using paints, epoxies and polymers.Key words: Microalgae, Biodeterioration, Colonization and Concretes
      Issue No: Vol. 34, No. 1
  • Partial purification and characterization of an inducible extracellular
           β-Glucosidase of Aspergillus niger IMI 502691

    • Authors: S. A. Adegoke, F. J. C. Odibo
      Abstract: β-Glucosidase (EC was produced by Aspergillus niger IMI 502691 using solid state fermentation of cassava root fibre. The enzyme was partially purified and characterized. The enzyme extracted using 20mM phosphate buffer pH 6.8 was concentrated to 10ml with 5M sucrose solution using dialysis membrane. It was purified from the culture medium by ion exchange chromatography on Carboxymethyl Sepharose and gel filtration chromatography on Biogel P 4. β- Glucosidase was purified 3.17 fold to give 3.68% yield relative to the total activity in the crude extract and with over 300% increase in specific activity of 106.93Umg-1 proteins for the partially purified protein. The enzyme exhibited decrease in total protein and total activity of 86.1% and 27.16%, respectively and optimal activity at pH 5.0 and 80oC (1h) in the absence of calcium. The β- glucosidase showed a wide range of pH and temperature of 3.0 to 7.0 and 50oC to 90oC, respectively. The enzyme was most stable at 50oC but retained above 50% of its activity for 1h at 60oC. The β-glucosidase of Aspergillus niger IMI 502691 was significantly activated by Sr2+, Fe2+, Mn2+, Ni2+, Zn2+and Cu2+ tested except for Ca2+ which inhibited the enzyme.Keywords: Glucosidase, chromatography,extract,activity, inhibited
      Issue No: Vol. 34, No. 1
  • A study on 3-mercaptopyruvate sulphurtransferase (3-MST) produced under
           submerged fermentation by Pseudomonas putida KT12440 isolated from an
           industrial effluent

    • Authors: A. P. Adekunle, N. Torimiro, R. E. Okonji
      Abstract: Effluents from an Iron and Steel company were collected, the cyanide level was determined and screening for cyanide degrading bacterial isolates on modified Bushnell Haas agar media was done. The amount of cyanide present in the industrial effluent was 1.06 mg/L. The 16S rRNA gene sequencing identified Pseudomonas putida KT12440 as the isolate with highest activity for 3-MST production. The cell growth was optimal at 30 h incubation time and a decrease in cell growth was observed with the increase of incubation time after the optimum. The highest 3-MST production was observed when mannitol was used as the sole carbon source while fructose is the least source of carbon. Casein showed the highest production for 3-MST when used as an alternative nitrogen source while NH4NO3 was the least. The optimal pH for 3-MST production was 9.0 and a sharp decrease in the enzyme production was observed thereafter. The incubation temperature for the production of 3-MST in the culture medium peaked at 30ºC and a sharp decline in temperature was observed afterward. The bacterial isolate screened in this study showed degradation potential that can be harnessed to remediate effluents containing cyanide. Keywords: Cyanide, Industrial Effluent, Pseudomonas putida KT12440, 3-MST
      Issue No: Vol. 34, No. 1
  • Haematology and serum indices of broiler starter fed graded levels of raw
           Pride of Barbados seedmeal

    • Authors: A. A. Ogunbode, P. T. Abegunde, S. A. Ayorinde, R. A. Adelere
      Abstract: A study was conducted to evaluate the haematology and serum indices of broiler starter fed graded levels of raw pride of Barbados (Caesalpina pulcherima) seedmeal. Seventy two day old Marshal broiler chicks obtained from Obasanjo Farms, Igboora, Oyo state, Nigeria were randomly assigned into four treatments, each treatment was replicated thrice with six birds per replicate in a completely randomized design, three diets were formulated containing raw pride of Barbados seedmeal at 5, 10 and 15% levels respectively. Blood samples were collected from six birds per treatment for haematological and six birds per treatment for serum-biochemical indices respectively. Results showed significant (P < 0.05) differences for White Blood Cell (WBC 103U/L), Red Blood Cell (RBC 106U/L), Packed Cell Volume (PCV %) and Haemoglobin (Hbg %) across the dietary treatments. Total Protein, Albumin, Globulin and Creatinine were significantly (P < 0.05) affected by the inclusion of raw pride of Barbados seedmeal in the diets.Keywords : Broiler starter, haematology, pride of Barbados seed, raw, serum, toxins
      Issue No: Vol. 34, No. 1
  • Microbiological quality assessment of facial cosmetics

    • Authors: O.H. Stanley, M. O. Immanuel, P. Ekanem
      Abstract:  Cosmetics are beauty enhancing agents for which microbial quality concerns have been neglected by users who often see them as innocuous. The aim of this study was to investigate the microbiological quality of selected facial cosmetic products. Thirty (30) samples each of in-use and unused cosmetics were obtained from users and cosmetics shops in Port Harcourt, Nigeria. The cosmetics sampled were lipsticks, eye shadows and foundations. The aerobic plate count and enrichment test methods were employed to isolate, enumerate and identify microbial contaminants using conventional presumptive and phenotypic identification methods. Microbial contaminants were present in 80% of the in-use cosmetics and 46.7% in the unused cosmetics. Results revealed presence of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp., Pseudomonas aeruginosa, Candida albicans, Aspergillus spp. and Penicillium spp in the in-use cosmetics and Staphylococcus aureus, Staphylococcus epidermidis, Aspergillus spp. and Rhizopus spp. in the unused cosmetics. Results revealed higher levels of contamination for in-use cosmetics with both pathogenic and non-pathogenic microbial species compared to the unused cosmetics. Results also indicated the failure of added preservatives to effectively inhibit microbial load to acceptable levels. Cosmetic use is on the increase and this study exposes the risk of using contaminated cosmetics products.Keywords: Cosmetics, pathogenic, microbial load  
      Issue No: Vol. 34, No. 1
  • Assessment of resistance status of some tomato genotypes to bacterial wilt
           disease and evaluation of SNP marker (LEOH19) for selection of BW
           resistant gene

    • Authors: S.A. Ganiyu, A.R. Popoola, O. A. Enikuomehin, J.G. Bodunde, O. B. Adedibu, A. U. Gurama
      Abstract: Marker assisted selection (MAS) has become very important and useful in selection of disease resistance genes in crop plants. Tomato, Solanum lycopersicum, is one of the most important vegetables worldwide but its production is being affected by pests and diseases, one of which is bacterial wilt caused by Ralstonia solanacearum. In endemic areas, the disease constitutes up to 100% yield loss. A-Two-season completely randomized design (CRD) experimental trial was conducted to assess the resistance status of forty (40) tomato genotypes. The results showed variations in resistance status, from highly resistance to highly susceptible. DNA concentrations from the assessed genotypes ranged between 14.46 and1430.52 ng/ul. Single Nucleotide Polymorphism (SNP) marker, LEOH19, was evaluated on the forty tomato genotypes for the identification of bw-gene that confers resistance to R. solanacearum. Genomic DNA was amplified using the primer sequence; (forward primer; 5´- AAGGCTCAGAAAGGGTCCAT-3´, reverse primer; 5´- GAGTTCATCAACACATCACACA-3´). The primer pairs produced amplification at 300 bp in 35 genotypes. After digestion, the product produced 300 bp in 33 genotypes. In screenhouse study, AVTO9803, AVTO0201, Tomachiva and Eyetom were found highly resistant and could be considered good materials in grafting and breeding programme for bacterial wilt resistance development. The results indicated that the primer amplified the specific sequence of the bw-gene locus in both the resistant and susceptible alleles. LEOH19 was found to be monomorphic and produced DNA fragments in both resistant and susceptible tomato genotypes and could, therefore, not be used as a molecular marker for marker assisted selection in tomato breeding programme.Key words: Bacterial wilt, Ralstonia solanacearum, resistance, SNP, Tomato.
      Issue No: Vol. 34, No. 1
  • Cellulase production by Proteus spp. JC402 from plantain fruits stalk
           biomass using submerged fermentation

    • Authors: M. B. Osho, A. O. Aruoture, T. A. Abatan
      Abstract: Biodegradation of lignocellulosic biomass into a variety of value - added products (monomeric sugars) by the action of cellulolytic enzymes has recorded a significant value for industrial processes. This present work aimed at isolating and screening bacteria of soil origin capable of producing extracellular cellulase, and investigated hydrolysis of plantain fruit stalk biomass (PFSB) using submerged fermentation. Alkaline pretreated and untreated PFSB substrates were used for the cellulase production. Enzyme extract was assayed using carboxyl methyl cellulose assay method. Out of eight bacteria species screened for cellulase producing potential, four showed positive on screening medium indicated by clear or halo zone around growing microbial colonies. Proteus spp. JC402 was selected based on highest zone of inhibition (23 mm) on screening medium and 100% hydrolysis efficiency. Cellulase activity of pretreated PFSB 7.5% (w/v) substrates has the highest activity 3.2 ± 0.14 Uml-1 and untreated PFSB 2 % (w/v) has 2.76 ± 0.31 Uml-1 at 48 h. Cheap, unexploited, renewable and readily available agricultural cellulosic biomass has been effectively utilized for the production of a thermo stable enzyme as criteria for industrial application and environmental implications.
      Issue No: Vol. 34, No. 1
  • Determination of amylose content and expression analysis of the
           Granular-Bound Starch Synthase I (GBSS1) gene in rice grains

    • Authors: A. O. Oko, A. Kumar, M. K. Lal, S. G. Sharma
      Abstract: The study determined amylose content and expression level of GBSS I gene in rice grain. A total of eighteen (18) rice (Oryza sativa L) cultivars of Indica varieties were collected from the National Rice Research Institute (NRRI) Cuttack and screened for their percentage amylose contents. Three cultivars namely Bindli, Naveen and Pooja which had contrasting characters in percentage amylose were then planted and used for the expression analysis. In the first stage of grain filling Bindli had 0.27 fold expression while Pooja and Naveen had 1.72 and 1.44 respectively. There was dramatic increase in the expression levels of the gene in the middle stage of grain filling in all the three cultivars with relative folds of 10.97, 42.71 and 27.34 recorded in Bindli, Pooja and Naveen respectively. However, at the last stage of grain filling, GBSS1 expression was low compared to the second stage with expression folds of 7.12, 7.55 and 1.67 in Bindli, Pooja and Naveen cultivars respectively. On the whole, Bindli cultivar showed lowest expression of the gene (GBSS1) when compared to Pooja and Naveen.Key words: rice gene, amylose, expression analysis, GBSS1  
      Issue No: Vol. 34, No. 1
  • Decolouration of laboratory dyes by immobilized cells of Pseudomonas
           aeruginosa and Bacillus subtilis at different carbon sources

    • Authors: O. B. Akpor
      Abstract: Dyes are indicated as one of the most problematic compounds in industrial effluents. This is due to their high solubility and low degradability. The aim of the study was to evaluate the effect of external carbon source on decolouration of bromothymol blue, crystal violet and methylene blue by alginate immobilized cells of Bacillus subtilis and Pseudomonas aeruginosa. A total of five carbon sources (fructose, sucrose, glucose, methanol, lactose and sodium acetate) were used for the study. The study revealed highest decolouration of the bromothymol blue occurring in media that contained sodium acetate as external carbon source. Decolouration rate of bromothymol blue was observed to increase from 25.49 % and 18.17 % (at 24 h incubation) to 51.49 % and 31.94 % (at 144 h incubation), in presence of the Bacillus subtilis and Pseudomonas aeruginosa immobilized cells. For decolouration of the crystal violet, glucose and sodium acetate were observed to be the most appropriate carbon sources. With glucose as carbon source in the media, decolouration rates of 57.48 % and 41.23 % at the end of incubation were observed for the crystal violet dyes in presence of the Bacillus subtilis and Pseudomonas aeruginosa cells, respectively. None of the carbon sources used for the investigation was found to enhance decolouration of the methylene blue by the test bacteria species. The study revealed the possible application of the immobilized cells in scale up studies for the remediation of textile effluents.Keywords: Decolouration,Dye,Immobilizedbacteria,Wastewater
      Issue No: Vol. 34, No. 1
  • Molecular characterisation of upland Ofada cultivars and NERICA rice for
           drought tolerance and their genetic relationships

    • Authors: O. S. Sakariyawo, G. D. Adesina, S. G. Aderibigbe
      Abstract: Tolerance to water deficit had been reported in Ofada and NERICA rice. The genetic basis of this is unknown. The objective of this experiment was to characterise the genetic basis for tolerance of upland Ofada and NERICA rice to water deficit and determine their genetic relationship. Genomic studies were conducted on upland Ofada rice genotypes (Golden red, straw gold, FUNAABOR 1 and 2) and NERICA rice (NERICA 7 and 8). Among the seven simple sequence repeats markers (RM 212, 36, 3805, 201 and 1115) genetically evaluated two (RM 72 and RM 316) were selected based on their polymorphism (Polymorphic information content and markers index). They both exhibited QTL brt8-12 and QRsf9 in RM 72 and RM 316 respectively, known for drought tolerance. Upland rice cultivars Golden Red, NERICA 8 and NERICA 7 exhibited higher QTL brt8-12 based on their gene ladder more than others on the RM 72 primer. NERICA 7, FUNAABOR 1 and FUNAABOR 2 occupied the highest DNA ladder in the marker RM 316, suggesting the high presence of the QTL QRsf9. Golden Red and NERICA 8 had similar genetic relationship and are better utilised with those of dissimilar sources (Straw Gold) to ensure desirable genetic variation.Keywords: DNA marker, genetic crop improvement, polymorphic information content, Quantitative trait Loci (QTL), Simple Sequence Repeats (SSR),
      Issue No: Vol. 34, No. 1
  • Association of IGF 1 gene polymorphisms with some morphometric traits of
           Nigerian indigenous sheep breeds

    • Authors: A. O. Raji, A. Mohammed, J. U. Igwebuike, C. Alphonsus
      Abstract: The relationship between IGF 1 gene polymorphisms and some morphometric traits of the Balami, Uda and Yankasa sheep breeds of Nigeria was investigated. Blood samples and morphometric measurements were obtained from 150 sheep (50 for each of the three breeds) at the Maiduguri Livestock market and abattoir while DNA was extracted at the Biotechnology laboratory of the University of Maiduguri. Evaluation of results revealed 2 alleles (A and B) and 3 genotypes (AA, AB and BB) for all the breeds. The Uda and Yankasa breeds had higher frequency of allele B (0.64 and 0.56, respectively) while Balami had higher allele frequency for A (0.61). Balami and Yankasa had high heterozygosity for IGF-1 gene while Uda had high homozygosity for B. For Balami sheep, the genotype BB had higher (P < 0.05) body weight and heart girth (56 kg and 79 cm, respectively) than AB for body weight (45.80 kg) and BB for heart girth (69.67 cm). Conversely, genotype AA had higher body length (94.33 cm) than AB (73.80 cm). IGF 1 gene polymorphisms did not significantly (P < 0.05) affect most morphometric traits of the Yankasa and Uda sheep except height at withers where the genotype AB had higher (P < 0.05) height at withers (71.77 cm) than AA (53.50 cm) for Yankasa sheep. Thus, the three breeds were found to be 100% polymorphic at the regulatory region of IGF-1 locus and this gene may be used as a marker for some morphometric traits in Nigerian indigenous sheep.Keywords: body weight, heart girth, height at withers, breeds, sheep
      Issue No: Vol. 34, No. 1
  • Evaluation of the effect of Cissus populnea aqueous root infusion on some
           testicular function indices and serum hormones of male rats

    • Authors: T. D. Olaolu, D. E. Rotimi
      Abstract: This study aimed at investigating the effect of aqueous infusion of Cissus populnea root on testicular function indices and serum hormones of male Wistar rats. Twenty male albino rats, weighing 130 ±25 g were completely randomized into 4 groups of five each. Animals in groups I to IV received 0.2ml of distilled water, 0.05 ml, 0.1 ml & 0.2 ml/kg body weight of the infusion, respectively. The oral administration was done once daily for 14 days using oropharyngeal cannula. Compared with the control, administration of aqueous infusion of C. populnea root at the doses administered resulted in significant (P < 0.05) increase in percentage testesbody weight ratio, serum testosterone, luteinizing and follicle stimulating hormones, acid phosphatase, alkaline phosphatase, testicular cholesterol, protein, sialic acid and glycogen. The results were observed to be 1.73 %, 3.4 ng/ml, 4.2 mlu/ml, 15.5 mlu/ml, 16.8 nM/min/mg protein, 3.39 mmol/ L, 13.58 nM/min/mg protein, 92.75 mg/ml, 1.97 mg/g and 8.38 mg/100mg glucose in the animals respectively compared with the control values of 1.10 %., 1.3 ng/ml, 3.5 mlu/ml, 5.64 nM/min/mg protein, 0.77 mmol/L, 2.58 nM/min/mg protein, 51.58 mg/ml, 1.09 mg/g and 3.13 mg/100mg glucose respectively. The oral administration of aqueous infusion of C. populnea root may help to improve fertility in males due to its ability to increase serum testosterone and gonadotropins secretion.Keywords: Cissus populnea, Testicular function indices, Serum hormones, Rats, aqueous infusions.
      Issue No: Vol. 34, No. 1
  • Effect of dietary inclusion of cashew nut shell liquid on metabolic
           functions of rumen bacteria in West African dwarf goats

    • Authors: R. Y. Aderinboye, J. Njuguna, E. Machuka, K. O. Adebayo, K. O. Olagoke, F. Stomeo
      Abstract: This study investigated the effect of dietary inclusion of cashew nut shell liquid (CNSL) on rumen microbial metabolic functions in West African dwarf goats. Twenty-four West African dwarf goats were divided into four groups of six animals each. Each group of animals was fed a basal diet of Panicum maximum and supplemented with one of four concentrate pellets containing CNSL at the rate of 0, 2, 4 and 6 ml/kg in a completely randomized design. Rumen fluid samples (n=6/animal) were collected from each animal after 60 days of feeding to assess the rumen metabolic functions in response to CNSL using the 16S rRNA metagenomic approach. Results revealed that the function of ammonia oxidizers was prominent while chitin degradation ranked least. The inclusion of 4 and 6 ml/kg of CNSL in supplemental diets for goats increased (P < 0.05) the function of xylan degraders while ammonia oxidizers, dehalogenation, nitrite reducers, methanogens amongst others were unaltered (P > 0.05). It can be concluded that 4 6 ml/kg of CNSL in supplemental pellets for goats enhanced xylan degraders implying higher hemicellulolytic functions which has implication for increased hemicellulose degradation in the rumen.
      Issue No: Vol. 34, No. 1
  • Effect of Physico-chemical Factors and Plankton Abundance in Selected
           Tropical Fish Earthen Ponds' Water

    • Authors: M. N. Chukwu, E. S. Afolabi
      Abstract: The study investigated the plankton diversity and abundance of three selected on-farm research fish ponds in Nigerian Institute for Oceanography and Marine Research, Lagos. Samples were taken from three ponds using standard procedure and physico- chemical features of the water bodies were measured. Plankton of class (Bacillariophyceae, Chlorophyceae, Cyanophyceae and EuglenophyceaeI) and Zooplanktons of (Cladocera, Rotifera and Copepoda) family were found. There were varying results for the phytoplaktons and zooplaktons respectively during the study. The highest percentage composition for phytoplankton was recorded in Pond 1 (94.11%), and the highest Shannon-Weaner diversity (1.265%) in pond 2. The highest percentage of zooplankton was recorded in pond 2 (91.67%). Utilizing the parameter of Shannon index value, the abundance value for zooplaktons was not remarkable. Statistical association between parameters and abundance reflected that abundance was positively correlated with temperature, and negatively correlated with pH while the levels of dissolved oxygen (DO) were positively correlated. The low densities and plankton diversity in pond 3 was an indication of low pH and high transparency as a result of nutrient depletion.Keywords: Abundance; physico-chemical factors; phytoplankton; zooplankton; fish ponds
      Issue No: Vol. 34, No. 1
  • Biodecolourization of dye-contaminated textile effluents using Bacillus
           cereus N27

    • Authors: T.T. Ayisa, S. B. Oyeleke, O. A. Oyewole, B. B. Adamu, Z. Umar, W. C. John
      Abstract: This study examined the potentials of Bacillus cereus N27 to decolourize textile effluents. The determined physicochemical parameters of the effluents were temperature, pH, total dissolved solids (TDS), total suspended solids (TSS), dissolved oxygen (DO), biological oxygen demand (BOD) and chemical oxygen demand (COD) using standard techniques. The Bacillus cereus N27 was isolated from textile effluent contaminated soil samples in Challawa, Kano state, Nigeria and were characterized and identified based on cultural, biochemical and molecular techniques. The Bacillus cereus N27 was used for the biodecolourization study. The 3 ml broth culture of Bacillus cereus N27 was inoculated into medium comprising 2 g of yeast extract, 2 g of glucose, 2.8 g of lactose broth in 50 ml of different concentrations of the effluent (0%, 5%, 10%, 30%, 50%, 70%, 90% and 100 %) and were incubated for 30 days at 37oC. The growth of the Bacillus cereus N27 was compared with corresponding McFarland standards. The rate of biodecolourization was determined using spectrophotometer at 600 nm. The effects of temperature (30oC, 35oC, 40oC, 45oC) and pH (4, 6, 8, and 10) on the decolourisation potential of the isolates was also determined. The results obtained for the physicochemical study were temperature (33.5oC), pH (6.10), BOD (0.13 mg/L), COD (123.5 mg/L). DO (0.162 mg/L), TSS (310 mg/L), TDS (465.5 mg/L). The optimum biodecolourization temperature was 35oC with 72.2% rate of biodecolourization after 30 days while pH 6 was optimum for the biodecolourization. The results obtained in the study suggest that Bacillus cereus can be used to develop an effective biological treatment system for the wastewaters contaminated with textile effluent.Keywords: Textile effluent, dye, biodecolourization,Bacillus cereus.
      Issue No: Vol. 34, No. 1
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Tel: +00 44 (0)131 4513762
Fax: +00 44 (0)131 4513327
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