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BIOTECHNOLOGY (227 journals)                  1 2 | Last

Showing 1 - 200 of 227 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 7)
Advances in Bioscience and Biotechnology     Open Access   (Followers: 15)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 8)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 8)
American Journal of Polymer Science     Open Access   (Followers: 30)
Animal Biotechnology     Hybrid Journal   (Followers: 10)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 62)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 5)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 2)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 1)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 5)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 5)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 6)
Biomédica     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 7)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Bioprinting     Hybrid Journal  
Bioresource Technology Reports     Hybrid Journal  
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 22)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 7)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 5)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 160)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 14)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 7)
Biotechnology for Biofuels     Open Access   (Followers: 11)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 16)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 2)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Biotribology     Hybrid Journal  
BMC Biotechnology     Open Access   (Followers: 16)
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 14)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 9)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access   (Followers: 1)
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 13)
Food Science and Biotechnology     Hybrid Journal   (Followers: 9)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 1)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 1)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 15)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 2)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 6)
Journal of Applied Biomedicine     Open Access   (Followers: 3)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 10)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 7)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 3)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 14)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 2)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 6)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 5)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 16)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 3)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 521)
Network Modeling and Analysis in Health Informatics and Bioinformatics     Hybrid Journal   (Followers: 3)
New Biotechnology     Hybrid Journal   (Followers: 4)
Nigerian Journal of Biotechnology     Open Access  
Nova Biotechnologica et Chimica     Open Access  
NPG Asia Materials     Open Access  
npj Biofilms and Microbiomes     Open Access  
OA Biotechnology     Open Access  
Plant Biotechnology Journal     Open Access   (Followers: 10)
Plant Biotechnology Reports     Hybrid Journal   (Followers: 4)
Preparative Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)

        1 2 | Last

Journal Cover HAYATI Journal of Biosciences
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 1978-3019 - ISSN (Online) 2086-4094
   Published by Bogor Agricultural University Homepage  [16 journals]
  • Proteogenomics: New Emerging Technology

    • Authors: Wasim Sajjad, Muhammad Rafiq, Barkat Ali, Muhammad Hayat, Sahib Zada, Wasim Sajjad, Tanweer Kumar
      Pages: 97 - 100
      Abstract: The term proteogenomics is basically the integration of proteomics with genomics and transcriptomics. Today, proteogenomics is developing on the way to combined understanding about overall cellular functions. At present globally, structure of genes, expression of genes (in the form of mRNA synthesis), synthesis of proteins (translation of mRNA) and post-translational modification (structural modification of proteins) have turn out to be technically practicable and act as a novel viewpoint to molecular procedures. Current research has proved the importance of proteogenomics technology in cancer for studying molecular signature of tumors particularly in human beings, and its treatment and prevention. Proteogenomics is not restricted to oncology but it also plays a vital role in other areas of life sciences and biomedicines and anticipation can make up these areas. Here in this minireview, we will discuss the latest progress made in recent years, challenges and viewpoints about proteogenomics technology.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Two Newly Recorded Species of the Lobster Family Scyllaridae (Thenus
           indicus and Scyllarides haanii) From South of Java, Indonesia

    • Authors: Yusli Wardiatno, Agus Alim Hakim, Ali Mashar, Nurlisa Alias Butet, Luky Adrianto
      Pages: 101 - 105
      Abstract: Two species of slipper lobster, Thenus indicus Leach, 1815, and Scyllarides haanii De Haan, 1841, are reported for the first time from the coastal waters of South of Java, part of the Indian Ocean. A total of two specimens, one specimen of T. indicus from Palabuhanratu Bay and one specimen of S. haanii from Yogyakarta coastal waters, were collected in April and September 2015, respectively. Descriptions and illustrations of the morphological characteristics of the two species and their habitat are presented.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Semi Intensive and Semi Biofloc Methods for the Culture of Indian White
           Prawn, Fenneropenaeus indicus in High-density Polyethylene Liner Ponds

    • Authors: Indra Effendy, Samy Al Deen, Sambhu Chithambaran
      Pages: 106 - 110
      Abstract: An experiment (triplicated) was conducted to assess the growth and production of Indian white prawn, Fenneropenaeus indicus in semi intensive and semi biofloc culture technique for a period of 120 days in polythene liner ponds (300 m2). Water exchange was done at 10% in semi intensive culture method (control) and zero water exchange was done in semi biofloc method (treatment). Soya hull and molasses were added as carbon sources to induce biofloc formation in treatment ponds. Post larvae (PL20) were stocked at the rate of 100/m2 in each pond and fed with a standard shrimp feed. Shrimp growth, physico-chemical parameters of water, bacteria, phytoplankton and zooplankton population, immune response and physical quality of shrimp were recorded during the period. Significant difference (p < 0.01) in shrimp growth (weight) was observed between control and treatment. Phytoplankton population and total haemocyte count were found to be increased and pathogenic bacteria population decreased in treatment ponds. Strong linear relationship was found between growth and biofloc content in treatment ponds. Shrimp grown in biofloc pond showed better colour and taste compared to control. Semi biofloc technique can be considered as an ideal culture method for bio secure production of white shrimp in semi arid lands.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene
           in Escherichia coli

    • Authors: Hamdayanty Hamdayanty, Sri Hendrastuti Hidayat, Tri Asmira Damayanti
      Pages: 111 - 116
      Abstract: Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available. The research was conducted to produce antigen of SCSMV coat protein (SCSMV-CP) through overexpressing it on bacterial expression which will be used for antiserum production. SCSMV-CP was amplified using specific primers for CP gene containing BamHI and HindIII restriction enzyme sites and cloned into pTZ57R/T. Subsequently, the SCSMV-CP was subcloned into pET28a and transformed on Escherichia coli BL21(DE3) and Rosetta-gami(DE3)pLysS. The concentration of isopropyl β-d-thiogalactopyranoside (IPTG), incubation temperature, and bacterial harvesting time after IPTG induction were optimized. SCSMV-CP gene was successfully amplified with size ±855 bp, subcloned into vector expression, and expressed in insoluble fraction either in both bacterial host. Optimal protein expression of SCSMV-CP recombinant was obtained at 25°C with IPTG concentration 0.25–1.00 mM and harvested at 9–12 hours after IPTG induction in E. coliBL21(DE3), and at 30°C with IPTG concentration 0.25–1.00 mM and harvested 3–12 hours after IPTG induction in E. coli Rosetta(DE3)pLysS. SDS-PAGE analysis showed that protein size of SCSMV-CP recombinant was ±35.4 kDa.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Elimination of Chloramphenicol by Tiger Shrimp (Penaeus monodon) and White
           Shrimp (Litopenaeus vannamei)

    • Authors: Heny Suseno, Sumi Hudiyono, Muslim Muslim
      Pages: 117 - 120
      Abstract: Chloramphenicol (CAP) has been illegally used in many shrimp farms in South East Asia, including Indonesia. We performed an experiment of elimination simulation of CAP in tiger shrimp (Penaeus monodon) and white shrimp (Litopenaeus vannamei). After 5 days of depuration process, the concentration of CAP in P. monodon decreased to 94.85% (muscle), 97.98% (cephalothoraxes), and 90.30% (exoskeleton). The elimination half-life of CAP in P monodon was 0.596 day in the muscle, 0.716 day in cephalothorax, and 0.437 day in exoskeleton. On the other hand, concentrations of CAP in L. vannameidecreased to 97.74% (muscle), 90.30% (cephalothoraxes), and 97.63% (exoskeleton). The elimination half-life of CAP in L. vannamei was 0.6624 day (muscle), 0.859 day (cephalothorax), and 0.796 day (exoskeleton). CAP was retained better by P. monodoncompared to L. vannamei.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Cognitive Style and Creative Quality: Influence on Academic Achievement of
           University Students in Indonesia

    • Authors: Nurul Huda Wulandari, Kanthi Arum Widayati, Bambang Suryobroto
      Pages: 121 - 124
      Abstract: Reasoning is a process to solve the problem. This process needs cognitive functions in thinking, learning, and making decision. Cognitive style is a term to explain human natural preferences in gathering and processing information. Humans appears to reason using two cognitive processing styles; the first system is called as intuitive thinking style that is spontaneous, effortless, and without conscious search, whereas the second system is called as reflective or analytical thinking that works in a deliberate, analytical, procedural, and controllable process. In human context, sometimes people encounter difficult problem or unknown situation that have to be coped by ideas that are both novel and adaptive to the task constraints. People who solve the problem successfully are called creative. Creativity is the base to enhance competitiveness among students that might result in good academic performance. The present study examined cognitive style and creative quality in affecting academic achievement of university students in Indonesia. The result showed that students who used analytical thinking tended to have higher academic success, especially in life science majors. Moreover, it was found that students would need to materialize their creative potential to reach greater academic achievement in demanding classes; for instance, the final year of undergraduate program.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Production of Xylanase by Recombinant Bacillus subtilis DB104 Cultivated
           in Agroindustrial Waste Medium

    • Authors: Is Helianti, Maria Ulfah, Niknik Nurhayati, Dadang Suhendar, Anita Kusuma Finalissari, Agustin Krisna Wardani
      Pages: 125 - 131
      Abstract: A recombinant Bacillus subtilis DB104 strain harbouring recombinant plasmid pSKE194 containing an Open Reading Frame (ORF) of endoxylanase and its indigenous promoter from the wild-type B. subtilis AQ1 strain was constructed. This recombinant B. subtilisDB104 strain had higher endoxylanase activity than the nonrecombinant B. subtilisDB104 strain in standard media, such as Luria Bertani (LB) and LB with xylan. The agroindustrial wastes corncobs and tofu liquid waste were chosen as cost-effective carbon and nitrogen sources, respectively, to test the economics of xylanase production using the recombinant B. subtilis DB104 at a larger scale. Submerged fermentation using a 4.5 L working volume fermentor with tofu liquid waste and 4% corncobs produced maximum xylanase activity of 1296 ± 1.2 U/mg (601.7 ± 0.6 U/mL) after 48-hour fermentation at 37°C with 150 rpm agitation; this is more than twofold higher than the activity produced in an Erlenmeyer flask. This is the first report of high xylanase activity produced from recombinant B. subtilis using inexpensive medium. During fermentation, the xylanase degrades corncobs into xylooligosaccharides, showing its potential as an enzyme feed additive or in xylooligosaccharide production.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Butterflies (Lepidoptera: Papilionoidea) of Mount Sago, West Sumatra:
           Diversity and Flower Preference

    • Authors: Ratih Rusman, Tri Atmowidi, Djunijanti Peggie
      Pages: 132 - 137
      Abstract: Diversity of butterfly depends on the availability of food plant and habitat quality. The aims of this study were to assess diversity and flower preference of butterflies in Mount Sago, West Sumatra, Indonesia. Observations were performed by scan sampling method in four types of habitats, i.e. secondary forests, pine forests, rubber forests, and agricultural areas. Our results showed that at least 184 species of butterflies and 56 species of nectar plants have been identified in Mount Sago. Nymphalid butterfly, Neptis hylas, was the most dominant species of butterflies. Two species, i.e. Trogonoptera brookiana and Troides amphrysus, were found as the protected butterflies. Diversity of butterfly in secondary forests was highest, whereas the lowest diversity was found in pine forest. Papilionid butterflies were found frequently feeding on tube blossoms, nymphalid butterflies on head blossoms, and lycaenid butterflies on flag blossom. Our results also showed that habitat change impacts composition and diversity of butterfly.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Alteration of Leaf Anatomy of Handeuleum (Graptophyllum pictum L. Griff)
           due to Gamma Irradiation

    • Authors: Arrin Rosmala, Nurul Khumaida, Dewi Sukma
      Pages: 138 - 142
      Abstract: The leaves of the plant handeuleum (Graptophyllum pictum L. Griff) have long been used for traditional medicine in several regions in Indonesia. This study was aimed to determine the effect of gamma irradiation rate on the anatomy and phytochemical content of the leaf. The rates of gamma rays used were 0, 15, 30, 45, 60, 75, 90, and 105 Gy. Our results showed that gamma ray irradiation rate of 30 Gy produced leaves that contain anthocyanins and carotenoids, with the highest number of stomata and stomatal density compared with control plants. Stomatal index was found highest in the leaves with 45 Gy of gamma irradiation. High-rate gamma ray irradiation produced rigid, thick, and frangible leaves. A high rate of gamma irradiation, i.e. 75, 90, and 105 Gy, produces bigger palisade, sponges, and upper epidermis than the control plants, respectively. Our results showed an association between increasing rate of irradiation with alterations in the structure of leaf anatomy and phytochemical content of handeuleum.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Gene Cloning and Protein Expression of Koi Herpesvirus ORF25

    • Authors: . Murwantoko, Cahya Kurnia Fusiyanto, . Triyanto
      Pages: 143 - 149
      Abstract: Koi herpesvirus (KHV) caused significant morbidity and mortality in koi and common carp (Cyprinus carpio). Glycoprotein has been used for vaccine development as sub unit vaccine against many viruses. KHV ORF25 is one of koi herpesvirus genes which encode a glycoprotein. The objectives of this research are to clone gene KHV ORF25and express its protein. The common carp showing necrosis and white patches of gill which was collected from Magelang was used in this research. Primers were designed to amplify partial ORF25 based on KHV J strain. KHV ORF25 was successfully amplified and cloned in pET32a. Sequence analysis showed that this KHV ORF25 has 99% homology with the sequences of KHV genotype KHV-J, KHV-I, and KHV-U. This ORF was predicted has 3, 23, and 8 B-cell epitopes based on Emini scale, Karplus and Schulz scale, and ElliPro respectively. The KHV ORF25 recombinant protein has been successfully produced in Escherichia coli as an insoluble protein with approximately 45 kDa in size. The high protein production was achieved when the protein induction was done at bacterial density at OD600 as 1.0 with 1-mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and incubated at 37°C for 18 hours. The protein predicted has immunogenicity and the potency as a vaccine is needed to be evaluated.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
  • Growth, Survival, and Body Composition of Transgenic Common Carp Cyprinus
           carpio 3rd Generation Expressing Tilapia Growth Hormone cDNA

    • Authors: . Kurdianto, . Alimuddin, Nurly Faridah, Goro Yoshizaki, Sri Nuryati, Mia Setiawati
      Pages: 150 - 154
      Abstract: Transgenic has been known as one of the applicable methods to improve growth performance of cultured fish. This study was performed to evaluate the growth performance, survival, and body composition of the 3rd generation of growth hormone (GH) transgenic common carp (TG). Juveniles (BW: 1.53 ± 0.03 g) were reared for 60 days in 250-L glass aquarium with stocking density of 25 fishes/aquarium. Fishes were fed with commercial feed (protein content 36%), three times a day to satiation. Growth and survival were measured every 20 days. Our results showed that TG fish has 1.49 times higher in average weight growth (p < 0.05) compared with the non-transgenic common carp (NT). Higher total feed consumption, survival, body protein content, protein and lipid retention, hepatosomatic index, and lower feed conversion ratio were also shown on TG fish compared with NT fish (p < 0.05). However, body lipid content and blood glucose level of TG fish were lower (p < 0.05) compared with the NT fish. Total ammonium nitrogen level in rearing media of TG fish was 51.78% lower (p < 0.05) than that of the NT fish. In conclusion, culturing of GH-TG common carp showed potential to achieve high productivity, efficient, and environmental-friendly aquaculture.
      PubDate: 2017-04-14
      Issue No: Vol. 23, No. 3 (2017)
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