for Journals by Title or ISSN
for Articles by Keywords
  Subjects -> BIOLOGY (Total: 3071 journals)
    - BIOCHEMISTRY (242 journals)
    - BIOENGINEERING (113 journals)
    - BIOLOGY (1453 journals)
    - BIOPHYSICS (46 journals)
    - BIOTECHNOLOGY (227 journals)
    - BOTANY (220 journals)
    - CYTOLOGY AND HISTOLOGY (28 journals)
    - ENTOMOLOGY (67 journals)
    - GENETICS (166 journals)
    - MICROBIOLOGY (261 journals)
    - MICROSCOPY (11 journals)
    - ORNITHOLOGY (26 journals)
    - PHYSIOLOGY (73 journals)
    - ZOOLOGY (138 journals)

BIOTECHNOLOGY (227 journals)                  1 2 | Last

Showing 1 - 200 of 227 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 7)
Advances in Bioscience and Biotechnology     Open Access   (Followers: 14)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 7)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 68)
American Journal of Bioinformatics Research     Open Access   (Followers: 8)
American Journal of Polymer Science     Open Access   (Followers: 29)
Animal Biotechnology     Hybrid Journal   (Followers: 9)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 62)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 5)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 2)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 8)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 1)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 5)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 5)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 6)
Biomédica     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 7)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Bioprinting     Hybrid Journal  
Bioresource Technology Reports     Hybrid Journal  
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 22)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 6)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 5)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 159)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 14)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 7)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 15)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 33)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 2)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Biotribology     Hybrid Journal  
BMC Biotechnology     Open Access   (Followers: 15)
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 3)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 3)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 14)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 9)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access   (Followers: 1)
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 12)
Food Science and Biotechnology     Hybrid Journal   (Followers: 9)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 1)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 1)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 15)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 2)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 6)
Journal of Applied Biomedicine     Open Access   (Followers: 3)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 10)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 7)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 3)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 14)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 2)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 6)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 5)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 16)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 3)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 520)
Network Modeling and Analysis in Health Informatics and Bioinformatics     Hybrid Journal   (Followers: 3)
New Biotechnology     Hybrid Journal   (Followers: 4)
Nigerian Journal of Biotechnology     Open Access  
Nova Biotechnologica et Chimica     Open Access  
NPG Asia Materials     Open Access  
npj Biofilms and Microbiomes     Open Access  
OA Biotechnology     Open Access  
Plant Biotechnology Journal     Open Access   (Followers: 10)
Plant Biotechnology Reports     Hybrid Journal   (Followers: 4)
Preparative Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)

        1 2 | Last

Journal Cover Journal of Plant Biochemistry and Biotechnology
  [SJR: 0.511]   [H-I: 20]   [6 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0971-7811 - ISSN (Online) 0974-1275
   Published by Springer-Verlag Homepage  [2351 journals]
  • Sequence analysis of coat protein and molecular profiling of sunflower
           necrosis virus (SNV) strains from Indian subcontinent
    • Authors: R. L. Chavhan; M. K. Verma; V. R. Hinge; U. S. Kadam; S. B. Kokane; Pranjib K. Chakrabarty
      Pages: 28 - 35
      Abstract: Sunflower is one of the leading edible oilseed crops of the world and is an important oil-producing crop of India. The sunflower necrosis disease caused by sunflower necrosis virus (SNV) has become a major hurdle for cultivation of sunflower in India. However, there is lack of genetic information and  standard methods for detection and identification of the SNV. To address this issue, we have developed an application using coat protein (CP) to perform molecular profiling of SNV strains. The nucleic acid and amino acid sequence analysis of CP of SNV strains collected from different regions of Maharashtra and Karnataka showed high percent homology (96.89–98.87%). However, 3D structural analysis generated eleven distinct groups of SNV strains.  Comparative bioinformatic analyses of nucleic acid and amino acid sequences with different genera of positive stranded (+) ssRNA viruses established their phylogentic relationship with ~25 (+) ssRNA viruses viz., Ilarvirus, Bromovirus, Cucumovirus, Alfamovirus, Comovirus, Nepovirus, Sequivirus, Potyvirus and Closterovirus. Additionally, the phylogenetic analysis revealed three distinct clusters, wherein major cluster I comprised SNV strains and Tobacco streak virus together showing 99% sequence homology and established closer phylogenetic relationship among all member viruses.
      PubDate: 2018-01-01
      DOI: 10.1007/s13562-017-0412-z
      Issue No: Vol. 27, No. 1 (2018)
  • Isolation, functional characterization and stress responses of raffinose
           synthase genes in sugar beet
    • Authors: Kunihide Kito; Koji Yamane; Takahiro Yamamori; Hiroaki Matsuhira; Yoshito Tanaka; Teruhiro Takabe
      Pages: 36 - 45
      Abstract: Raffinose (sucrosylgalactoside oligosaccharide) is a water soluble carbohydrate and accumulates in response to abiotic stresses in plants. Plant raffinose synthases are poorly characterized, and the genes involved in raffinose biosynthesis are unknown in sugar beet. Here, we report the isolation of two genes encoding raffinose synthase (BvRS1 and BvRS2) as well as a gene encoding galactinol synthase (BvGolS1) from sugar beet. BvRS1 and BvRS2 show high homologies to Arabidopsis raffinose synthase AtRS5. BvRS1 and BvGolS1 were expressed in Escherichia coli. Crude extracts showed the activities of raffinose synthase and galactinol synthase. The K m values of BvRS1 for galactinol and sucrose and the K m values of BvGolS1 for UDP-galactose and myo-inositol were determined. The expression levels of BvRS1 were significantly higher than that of BvRS2. The mRNA for BvRS1 was rapidly induced by cold stress whereas the mRNA for BvRS2 was slowly induced by cold and salt stresses. These data suggest that BvRS1 and BvRS2 encode raffinose synthase genes responsible to cold and salt stress, respectively.
      PubDate: 2018-01-01
      DOI: 10.1007/s13562-017-0413-y
      Issue No: Vol. 27, No. 1 (2018)
  • Transcriptomes of Podophyllum hexandrum unravel candidate miRNAs and their
           association with the biosynthesis of secondary metabolites
    • Authors: Pawan Kumar; Jibesh Kumar Padhan; Ashwani Kumar; Rajinder S. Chauhan
      Pages: 46 - 54
      Abstract: Podophyllotoxin is a high value metabolite having anticancerous activity and procured from a medicinal plant Podophyllum hexandrum. The escalating demand for podophyllotoxin necessitates development of alternate production platforms. Cell cultures have been tried in the past, however, podophyllotoxin yields were very low (0.3%). Multiple genes of biosynthetic pathway have been correlated with podophyllotoxin content. Understanding regulation of podophyllotoxin is, therefore, essential to design a suitable genetic intervention strategy. Six potential miRNAs were identified in NGS transcriptomes of P. hexandrum for which eight potential mRNA targets were discerned. The identified miRNAs and their mRNA targets (UDP glycosyltransferase, flavonol synthase, glyceraldehyde 3-phosphate dehydrogenase, peroxidase, malate dehydrogenase, phosphoenolpyruvate carboxylase, WRKY 37 and MYBF1 transcription factors) were further tested for any correlation through qRT-PCR. miR396b, miR2673a, miR828b and miR2910 established negative correlation with their mRNA targets through downregulation via complementary miRNA:mRNA base pairing. This is the first report on identification, characterisation, validation and expression analysis of miRNAs and their targets in P. hexandrum.
      PubDate: 2018-01-01
      DOI: 10.1007/s13562-017-0414-x
      Issue No: Vol. 27, No. 1 (2018)
  • Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine
           ammonia lyase can use exogenous tyrosine for p -coumaric acid production
    • Authors: S. Tantong; N. Nuengchamnong; S. Kumphune; A. Incharoensakdi; P. Lindblad; S. Sirikantaramas
      Pages: 118 - 122
      Abstract: Phototrophic cyanobacteria may be considered as an alternative host for producing numerous bioactive compounds. We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 ± 1.5 μg of p-coumaric acid per 108 cells (0.36 mg L−1). Additionally, our study is the first report to show the biotransformation of tyrosine to p-coumaric acid reaching a maximum 2.4-fold increase when 0.5 mM tyrosine was supplemented to the growth medium. Liquid chromatography-mass spectrometry analysis revealed the occurrence of diverse patterns of metabolites under different concentrations of supplemented tyrosine, suggesting that it is used in additional metabolic pathways.
      PubDate: 2018-01-01
      DOI: 10.1007/s13562-017-0416-8
      Issue No: Vol. 27, No. 1 (2018)
  • Comparative characterization of small RNAs derived from an emaravirus and
           a geminivirus infecting pigeonpea
    • Authors: Basavaprabhu L. Patil; Deepika Arora
      Abstract: High throughput sequencing technologies, supported by bioinformatics tools are employed to retrieve small RNA sequence information derived from the nucleic acids of plant infecting viruses. In addition to characterization of the small RNAs to understand the biology of the virus, the small RNA sequence can be assembled to reconstitute viral genome sequence. For the first time the semiconductor based Ion Proton sequencing technology is used to sequence the small RNAs from pigeonpea (Cajanus cajan) plants infected by two distinct viruses with RNA and DNA as their genomes. The reconstitution of the viral genome sequence revealed that the pigeonpea plant from Kalaburagi (erstwhile Gulbarga, Karnataka state) was infected by an emaravirus species Pigeonpea sterility mosaic emaravirus 1 (PPSMV-1) and another plant from New Delhi was infected by a begomovirus species Mungbean yellow mosaic India virus (MYMIV). Characterization and comparison of small RNA sequences derived from both the viruses showed vast differences in their pattern of accumulation and their size classes. In the case of PPSMV-1, the 21 nt sized siRNAs accumulated at far greater levels followed by 22 and 24 nt siRNAs. Whereas in MYMIV, the proportion of accumulation of each size class of siRNAs was similar. Further the distribution of small RNAs across the genomes of PPSMV-1 and MYMIV was mapped and the density of small RNA accumulation showed a positive correlation with the GC content of viral sequence.
      PubDate: 2018-03-03
      DOI: 10.1007/s13562-018-0447-9
  • Genetic diversity changes in Indian lentils over the times
    • Authors: Jitendra Kumar; Sunanda Gupta; Sonali Dubey; Priyanka Gupta; Debjyoti Sen Gupta; N P Singh
      Abstract: The genetic diversity of 96 genotypes of lentil comprising 34 cultivars, 46 advanced breeding lines, and 16 germplasm lines were studied using 260 SSR markers. These markers generated a total of 749 alleles. The alleles/locus ranged from 2 to 16 with an average value of 2.87. Polymorphic information content varied from 0.02 to 0.91 with a mean of 0.30. Major allelic frequency ranged from 0.14 to 0.99 with a mean of 0.77. Studied genotypes were clustered into two groups according to their breeding history. Advanced breeding lines derived from exotic lines were clustered in one group, while another group accommodated most of the cultivars and advanced breeding lines with common cultivars in parentage. The germplasm lines were sub-clustered within first group. Cumulatively, first three principal components contributed 21.2% to the total variability. Advanced breeding lines showed higher number of alleles/locus and gene diversity (He) than other sets of genetic materials. In present study, no significant differences were observed between cultivars developed in different decadal groups for both NA and He. Moreover, genetic diversity changes between small and large seeded lentil cultivars were also found non-significant in this study. These findings showed that the use of alien genes can help to diversify active gene pool for developing improved new cultivars in lentil.
      PubDate: 2018-03-03
      DOI: 10.1007/s13562-018-0450-1
  • A rice bZIP transcription factor, OsbZIP16 , regulates abiotic stress
           tolerance when over-expressed in Arabidopsis
    • Authors: Agni Shekhar Pandey; Eshan Sharma; Nitin Jain; Brinderjit Singh; Naini Burman; Jitendra P. Khurana
      Abstract: Plants exposed to adverse environmental conditions are invariably compromised in their growth and development. The bZIP class of transcription factors (TF) form a large group among stress signalling components that regulate plant responses towards stress. We identified bZIP TF encoding genes that are expressed differentially in indica rice under stress and here we functionally characterize one such gene, OsbZIP16. Although, OsbZIP16 forms a clade with its orthologous monocot protein sequences, we find in our study that it can impart tolerance to abiotic stress in Arabidopsis. OsbZIP16 is expressed strongly upon dehydration, salt and ABA treatment in Oryza sativa cv. IR64 seedlings. It localizes in the cell nucleus and the gene product is capable of transcriptional activation, thus providing evidence for its capability as a functional TF. Upon overexpression in Arabidopsis, OsbZIP16ox plants show wild type morphology, however, these plants showed tolerance when subjected to drought stress at vegetative stage and set healthy seeds on recovery. The OsbZIP16ox seedlings showed reduced sensitivity to mannitol, ABA and sodium chloride during germination and also reduced ROS accumulation upon H2O2 exposure. Thus, OsbZIP16 regulates abiotic stress responses and is also a good candidate gene that can be utilised to impart tolerance in plants under water deficit conditions.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0448-8
  • Isolation and expression analysis of eight MADS - box genes in peach (
           Prunus persica var. nectarina ‘Luxing’)
    • Authors: Hui-feng Li; Qing-long Dong; Hou-zhen Jia; Gui-xiang Li; Kun Ran
      Abstract: The MADS-box transcription factor (TF) plays a crucial regulatory role in plant vegetative growth, flower and fruit development. Eight MADS-box genes (designated as PpMADS15, 16, 17, 26, 27, 36, 37, 38; GenBank accession nos. KU559581, KU559582, KU559583, KU559592, KU559593, KU559602, KU559603, KU559604, respectively) were isolated from ‘Luxing’ (Prunus persica var. nectarina ‘Luxing’) peach by homologous comparison and RT-PCR, which contained open reading frames (ORF) of 597, 750, 1062, 615, 699, 1 107, 678 and 564 bp, respectively. The results of phylogenetic analysis revealed that PpMADS15 belonged to the AG subgroup, PpMADS16 to the SEP subgroup, PpMADS17 to the MIKC* group, PpMADS26, 27, and 38 to the Mα group, and PpMADS36 and 37 to the Mγ group. The results of the prediction for subcellular localization showed that eight PpMADS proteins were located in the nucleus. The results of promoter analysis indicated that there were multiple putative cis-acting elements that were involved in responsiveness to the following variables: light, defense and stress, low-temperature, heat stress, wound, fungal elicitor, anaerobic induction, MeJA, gibberellin, ABA, auxin, and SA. RT-PCR results showed that PpMADS15 was expressed in leaves, stems, roots, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS16 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS17 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development (except for 30 d). All members in the Mα and Mγ subgroups were expressed in roots, stems, leaves, sepals, ovaries, stamens, petals and during flower development, but PpMADS27 was expressed only during fruit development. These results suggested that eight PpMADS genes played a crucial regulatory role in vegetative growth, flower and fruit development of peaches.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0452-z
  • Expression patterns of the genes encoding fructan active enzymes (FAZYs)
           alongside fructan constituent profiles in chicory ( Cichorium intybus L.):
           effects of tissue and genotype variations
    • Authors: Iman Khaldari; Mohammad Reza Naghavi; Seyed Ali Peighambari; Jaber Nasiri; Fatemeh Mohammadi
      Abstract: Dynamic transcriptional variations of genes encoding fructan active enzymes (FAZYs; 1-SST, 1-FFT, 1-FEHI, 1-FEHII) alongside their potential contributions regarding production/degradation of various carbohydrates (i.e., fructose, glucose, sucrose, 1-kestose, and inulin) were scrutinized in the two distinct genotypes of chicory (Cichorium intybus L.) namely “Germany variety” and “Iranian landrace”, at flowering stage. Germany variety accumulated overall more amounts of fructose, sucrose, and inulin, while Iranian landrace contained the highest proportion of 1-kestose, and glucose. Furthermore, both 1-SST and 1-FFT genes were dramatically up-regulated in the root-harvested samples of both genotypes, though maximum transcripts of 1-SST and 1-FFT were respectively detected in landrace and Germany variety, suggesting a cultivar- and tissue-dependent phenomenon. Instead, in both genotypes, maximum transcript levels of 1-FEHI were detected in stem, while root, flower and leaf tissues possessed inferior magnitudes. Considering 1-FEHII, both genotypes exhibited a down-regulated behavior in four tissues (excluding landrace stem). Meanwhile, the chicory MYB transcription factor (CiMYB17) transcriptionally fluctuated among four tissues of both chicory genotypes, and exhibited significant correlation only with 1-FEHI in Germany, a moderate correlation with 1-FEHI in landrace, and lower associations with 1-SST, 1-FFT, and 1-FEHII, indicating its trivial regulatory roles in the complex regulation of FAZY gene expression, and subsequently biosynthesis/degradation of fructans under normal circumstances. Regarding landrace, both 1-SST and 1-FFT correlated only with glucose, while for Germany variety, 1-SST had a strong association with inulin, 1-ketose, alongside glucose, and 1-FFT had a strong correlation only with glucose. However, both 1-FEHI and 1-FEHII exhibited no considerable associations with all the carbohydrates studied. Notably, 1-FEHII negatively correlated with inulin content, indicating an “antagonistic” effect between inulin accumulation/production and 1-FEHII activity. The results, overall, demonstrated that variations in genotypes and/or tissues under study can synergistically/antagonistically influence expression patterns of FAZY genes alongside production/degradation of the corresponding fructans.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0454-x
  • Mapping quantitative trait loci for important agronomic traits in finger
           millet ( Eleusine coracana ) mini core collection with genomic and genic
           SSR markers
    • Authors: B. Kalyana Babu; Salej Sood; C. Chandrashekara; A. Pattanayak; Lakshmi Kant
      Abstract: Allele identification for agro-morphological traits and stress resistance is a major concern across the globe for improving productivity of finger millet. Here, we used 46 genomic and 58 genic simple sequence repeats (SSRs) markers in a set of 66 accessions used to constitute a global mini-core collection for analysing their genetic structure as a population and establishing association among markers and twenty morphological traits including resistance to finger blast. Phenotypic data revealed a wide range of variation for all traits except flag leaf width and flag leaf sheath width. We got amplification of 81 alleles by the 31 genomic SSRs at an average of 2.61 alleles per locus. Polymorphism information content (PIC) values varied from 0.21 to 0.75 and average gene diversity was 0.49. Structure analysis of the population using the genomic SSR data divided the accessions into two clusters where Indian and exotic accessions were grouped in separate clusters. Genic SSRs which were associated with blast resistance genes, amplified 36 alleles at an average of 2 alleles per locus. PIC values ranged from 0.32 to 0.37 and average gene diversity was 0.45. Population structure analysis using data from these SSRs grouped the accessions into three clusters, which broadly correspond to their reaction to blast disease. Twenty-two significant associations were found using the GLM approach for 20 agro-morphological traits both in 2012 and 2014, while, 7 and 5 significant marker-trait associations were identified using MLM in 2012 and 2014 respectively. The SSR markers FMBLEST35 and FMBLEST36 designed from the Pi21 gene sequence of rice were found to be associated with blast disease resistance in finger millet indicating that the gene homologues play a significant role in an important role for neck blast resistance.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0449-7
  • Detection of pea wilt pathogen Fusarium oxysporum f. sp. pisi using
           DNA-based markers
    • Authors: Kamal Dev Sharma; Hemlata; Rajeev Rathour; R. K. Kapila; Y. S. Paul
      Abstract: Identification of the fungus Fusarium oxysporum f. sp. pisi (Fop), the causal organism of wilt disease of pea, is a time consuming and arduous task. Diagnosis of Fop by traditional means requires more than 2 months and involves two steps, identification of species using morphological characters and formae specialis ‘pisi’ using pathogenicity assays. The ambiguous morphological differences between F. solani and F. oxysporum further complicate the diagnosis of F. oxysporum. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) based method was developed to detect Fop from India. A PCR–RFLP marker, HPACAPS1380, generated after restriction of 28S rDNA region with enzyme MvaI, detected accurately the Fop among several other fungi with detection sensitivity of 5 fg of Fop genomic DNA. In a mixture of Fop and pea DNA, the sensitivity was 500 pg of Fop DNA in 50 ng of pea DNA. The assay was further refined to detect the Fop from infected tissues and infested soil. The current assay can detect Fop from culture, plant tissues and soil in a considerably shorter period of time compared to traditional methods.
      PubDate: 2018-02-13
      DOI: 10.1007/s13562-018-0443-0
  • In silico structural, functional and phylogenetic analysis of Klebsiella
    • Authors: Krishnendu Pramanik; Priyanka Pal; Tithi Soren; Soumik Mitra; Pallab Kumar Ghosh; Anumita Sarkar; Tushar Kanti Maiti
      Abstract: Phytic acid or phytate (myo-inositol hexakisphosphate) is the principal storage indigestible form of phosphorus in different crops. It is considered as an antinutrient in human as well as animal (including fish, poultry, pig, chicken etc.) diet due to its chelating behavior of certain essential divalent minerals (Fe2+, Mg2+, Zn2+, Ca2+ etc.). The unabsorbed, indigested form of phosphorus also causes phosphate pollution in the soil by animal wastes. Phytate degrading enzymes like phytases (myo-inositol hexakisphosphate phosphohydrolase) in this regard can be very useful and also economically feasible to reduce the risk of phosphate pollution and increase the nutrient value in animal feeds at the same time. The Klebsiella phytases are suitable to use in the food industries of plant origin for their excellent thermal stability and high pH tolerance. From the present in silico investigation, it was found that Klebsiella phytases were 46–47 kDa molecular weight protein of histidine phosphatase superfamily having thermostability and alkalinity nature. This thermostability can be achieved due to possession of higher percentage of α helices and β sheets at the same time; the presence of higher aliphatic indices (range in between 88 and 91) etc. Interestingly, a strong correlation was found to be pertinent from phylogenetic studies of proteins with their cDNA among both species and strain level. Hence, the present study would be beneficial for future researchers (3D model available in Protein Model Database with acc. no.: PM0080562) to meet the demand of agricultural and industrial production of bacterial phytases particularly for agricultural farming.
      PubDate: 2018-02-12
      DOI: 10.1007/s13562-018-0445-y
  • Protein changes in the shoot-tips of vanilla ( Vanilla planifolia ) in
           response to osmoprotective treatments
    • Authors: María Teresa González-Arnao; Armando Guerrero-Rangel; Octavio Martínez; Silvia Valdés-Rodríguez
      Abstract: Cryogenic storage of vanilla shoot-tips represents the safest biotechnological strategy for the long-term conservation of the vanilla germplasm, but successful cryopreservation depends on its tolerance to both dehydration stress imposed by cryoprotective treatments and thermal stress produced by immersion in liquid nitrogen. In this work, we evaluated the impact of various osmoprotective treatments on protein expression patterns in vanilla (Vanilla planifolia) shoot-tips subjected to successive dehydration steps prior to cryopreservation. Two-dimensional electrophoretic protein profiles of shoot-tips dissected from in vitro grown plants and preconditioned on semisolid media with 0.3 M sucrose for one day, and shoot-tips preconditioned, loaded with a solution of 0.4 M sucrose and 2 M glycerol, and subsequently exposed to plant vitrification solution 3 (50% (w/v) sucrose and 50% (w/v) glycerol), were compared with non-treated dissected shoot-tips. We observed an increase in the expression level of six protein spots (fold change exceeding 1.5) and a decrease (fold change not exceeding 0.6) of ten protein spots after preconditionig treatment, whereas the profiles after preconditioning, loading and exposure to vitrification solution showed an increase in the expression level of 21 protein spots and a decrease in the expression level of 13. Most proteins identified were down-regulated and belonged to groups of biosynthesis, folding, and protein degradation. Many others were related to energetic metabolism, defense, and cell structure. These preliminary results contribute to knowledge of the proteome of this species and partially clarify its sensitivity to osmotic dehydration treatments.
      PubDate: 2018-02-12
      DOI: 10.1007/s13562-018-0442-1
  • Isolation, characterization and analysis of the agglutinative activity of
           a lectin from Crotalaria spectabilis
    • Authors: Wilian Rosário de Oliveira; Evandro José Lima Rego; Paula Carvalhal Lage Von Buettner Ristow; Eudes da Silva Velozo; Diego de Carvalho Carneiro; Bruno Lopes Bastos; Suzana Telles da Cunha Lima
      Abstract: Lectins are proteins with ability to recognize specific carbohydrates. These are present in virtually all organisms and have increasing applications in biotechnology. Here, our aim was to purify lectins from seeds of Crotalaria spectabilis Roth and determine their agglutinative ability. In this study, 45 g of seeds were milled, their proteins were precipitated by acetone or ammonium sulfate and purified by exclusion and ion-exchange chromatography. An isolated lectin was submitted to tests for hemagglutination and inhibition of hemagglutinating activity by carbohydrates as well as tests for its response to chelating and reducing agents. Our results show that the apparent molecular weight (as determined by SDS-PAGE) of the lectin is 30 kDa, and the tests for inhibition of erythrocytes’ agglutinative activity by sugars were positive for d-galactose and N-acetyl-d-galactosamine. Data obtained with the chelating agent EDTA demonstrated the presence of divalent cations in the protein structure. However, the reducing agent 2-mercaptoethanol was unable to inhibit the protein’s bioactivity. The lectin agglutinated the blood groups A, B, AB and O, as well as bacterial lineages from the species Leptospira interrogans and Leptospira biflexa, indicating a prospective application in the diagnosis and treatment of leptospirosis.
      PubDate: 2018-02-08
      DOI: 10.1007/s13562-018-0446-x
  • Molecular and biochemical characterization of mungbean yellow mosaic India
           virus resistance in leguminous host Vigna mungo
    • Authors: Nibedita Chakraborty; Jolly Basak
      Abstract: Mungbean yellow mosaic India virus (MYMIV)—the causal agent of the yellow mosaic disease is responsible for severe damage of crops that are of great economic importance. In the current study, we explored the process of MYMIV infection and its natural resistance by analysing the expression of early and late viral genes at different time points in the leaves of resistant and susceptible Vigna mungo plants. Accordingly, we have periodically evaluated several biochemical parameters commonly associated with oxidative status of resistant and susceptible V. mungo plants during MYMIV infection. Our study revealed that accumulation levels of the early as well as late expressed genes of MYMIV were low and high in the resistant and susceptible plants, respectively; whereas membrane stability index (MSI) exhibited an opposite response. Moreover, a decrease in the malondialdehyde levels along with an increase in the activities/levels of different antioxidant enzymes, total phenol and H2O2 was noted during the early stages of infection in the resistant plants. Such observations argue in favour of strong defensive capability of the resistant plants in restricting the accumulation of viral RNA and generation of harmful free radicals within the studied tissue. Collectively, it appears that obstruction of viral invasion in plant cell wall, restriction in viral DNA replication, and early onset of antioxidant defense responses altogether might be responsible for MYMIV natural resistance. Such information is helpful in understanding the pathogenesis of MYMIV infection and its resistance in V. mungo and other economically important crops.
      PubDate: 2018-01-31
      DOI: 10.1007/s13562-018-0441-2
  • Genome-wide identification of major transcription factor superfamilies in
           rice identifies key candidates involved in abiotic stress dynamism
    • Authors: Pandiyan Muthuramalingam; Subramanian Radhesh Krishnan; Kadarkarai Saravanan; Narayanan Mareeswaran; Reetesh Kumar; Manikandan Ramesh
      Abstract: Transcription factors (TFs) are pivotal players in plant stress signaling and signal transduction pathways. Among the key TFs, NAC, ZF-HD, AP2-EREBP, WRKY and bHLH proteins play crucial roles in the regulation of reprogramming the transcriptome and associated responses in stress. Considering this, genome-wide identification of NAC, ZF-HD, AP2-EREBP, WRKY and bHLH TF families were performed in the C3 model plant, Oryza sativa. The computational study identified 144 NAC, 15 ZF-HD, 164 AP2-EREBP, 103 WRKY, and 135 bHLH proteins and their physicochemical properties and, expression profiling by computational analysis. Genome-wide in silico expression analysis of NAC, ZF-HD, AP2-EREBP, WRKY, bHLH genes showed their differential expression profiling in different tissues. Expression patterns, gene structure, subcellular localization, gene ontology of 17 NAC, 3 ZF-HD, 13 AP2-EREBP, 11 WRKY, 8 bHLH key genes suggested the putative novel variants in stress and signal transduction. These key players are needed to be studied in order to categorize and outline their functional roles in AbS signaling network.
      PubDate: 2018-01-31
      DOI: 10.1007/s13562-018-0440-3
  • Expression analysis on mycoparasitism related genes during antagonism of
           Trichoderma with Colletotrichum falcatum causing red rot in sugarcane
    • Authors: Elangovan Elamathi; Palaniyandi Malathi; Rasappa Viswanathan; Amalraj Ramesh Sundar
      Abstract: Present study was aimed to select a suitable Trichoderma isolate as candidate antagonist based on its efficacy in producing cell wall degrading enzymes (CWDEs), its mycoparasitism activity and expression of related genes against the red rot pathogen caused by Colletotrichum falcatum in sugarcane. For which, six different isolates of Trichoderma selected from our earlier studies (T. harzianum, T. asperullum) were evaluated based on their capability in releasing cell wall degrading enzymes individually and during antagonism with C. falcatum in dual plate. Amongst T. harzianum (T20) exhibited the greatest mycoparasitic potential against the C. falcatum, by producing higher concentration of  CWDEs viz., chitinase and β-1, 3-glucanase, slightly lower amounts of cellulase and protease with significant reduction in polygalacturonase produced by pathogen. Further microscopic observation on interaction of C. falcatum with the selected isolate of T. harzianum (T20) exhibited the mycoparasitic activity of antagonist over pathogen in dual culture and inhibition of C. falcatum pathogenesis in detached sugarcane leaves. In addition, expression pattern of eight genes coding various enzymes involved in mycoparasitism by T. harzianum over C. falcatum were analyzed using qRT-PCR in vitro and on sugarcane leaves. In in vitro interactions, five genes of  cell wall degrading enzymes viz., chitinase (chit33), endochitinase (endo42), β-1, 3-glucanase (glu), exochitinase 1 (exc1), exochitinase 2 (exc2), were upregulated during and after contact as compared to before contact, while three genes related with proteases such as alkaline proteinase (prb1), trypsin-like protease (Pra1), subtilin-like serine protease (ssp), genes were upregulated during the contact with C. falcatum and slightly down regulated after contact. In detached leaves, seven genes were potentially upregulated except subtilin-like serine protease, which was down regulated during interaction of C. falcatum and T. harzianum as compared to T. harzianum inoculation alone. All these biochemical and molecular results confirm the efficacy of T. harzianum (T20) against C. falcatum and justify the right selection of candidate antagonist for our further studies on identification of antifungal genes/proteins against C. falcatum in sugarcane.
      PubDate: 2018-01-30
      DOI: 10.1007/s13562-018-0444-z
  • Salicylic acid influences lenticel discolouration and physiological and
           biochemical attributes of mango ( Mangifera indica L.) fruits
    • Authors: K. Prasad; R. R. Sharma
      Abstract: Lenticel discolouration (LD) has now emerged as a leading postharvest threat in mango, which interferes with the face value of fruits, thereby affecting the trade and causing huge monetary losses to our country. For its management, we designed an experiment using salicylic acid at 200, 400 and 600 ppm concentration along with control fruits, as a dip treatment for 5 min. Our results revealed that salicylic acid at 200 ppm was not only effective in reducing LD significantly but also reduced the activities of polyphenol oxidase (PPO) (0.397 ∆A410 O.D min−1 g−1 FW), peroxidase (POD) (0.050 ∆ A470 O.D min−1 g−1 FW), and lipoxigenase (LOX) (3.227 µmol min−1 g−1 FW) enzymes and helped in increasing the total phenolics (15.46 mg gallic acid equivalent 100 g−1). This treatment also suppressed the rates of ethylene evolution (0.521 µL kg−1 h−1) and respiration (34.46 mL CO2 kg−1 h−1) over untreated mango fruits. With respect to quality parameters, the significant decrease in postharvest decay (23.3%) occurred without any adverse effect on soluble solids concentrates (16° B) and total carotenoids (4.1 mg 100 g−1pulp). Thus, keeping all parameters (physical, physiological, biochemical and quality) in view, salicylic acid at 200 ppm was most effective as a postharvest dip treatment for reducing LD in mango during storage or marketing without adversely affecting the fruit quality.
      PubDate: 2018-01-29
      DOI: 10.1007/s13562-018-0439-9
  • A modified in planta method of Agrobacterium -mediated transformation
           enhances the transformation efficiency in safflower ( Carthamus tinctorius
    • Authors: Arti Rani; Asha Panwar; Manjary Sathe; Anil Kush
      Abstract: Plant transformation has emerged as an important tool to integrate foreign genes in the plant genome to modify the plants for desired traits. Though many techniques of plant transformation are available; getting single copy transgenic events and cost associated remains a big challenge. Thus Agrobacterium-mediated transformation remains the method of choice due to multiple advantages. In the present work a tissue culture free protocol of Agrobacterium-mediated transformation was optimized in safflower, an oil seed crop recalcitrant to transformation. As a proof of concept we selected pCAMBIA2300 gene cassette containing Arabidopsis specific delta 15 desaturase (FAD3) downstream to truncated seed specific promoter beta-conglycinin and optimized tissue culture free protocol of Agrobacterium-mediated transformation using embryos as explants. Addition of silwet L-77, sonication treatment, vacuum infiltration in infection medium and use of paper wicks in co-cultivation period increased the transformation efficiency to 19.3%. Further, success in transformation was confirmed via product accumulation in 21 independent transgenic events wherein oil in transformed seeds showed significant accumulation of alpha-linolenic acid (ALA; 18:3; n3) which is generated from linoleic acid (LA; 18:2; n3) in a FAD3 catalyzed reaction. The present protocol can be utilized to produce transgenic safflower with different desired characters.
      PubDate: 2018-01-10
      DOI: 10.1007/s13562-017-0437-3
  • Microsatellite (SSR) marker assisted assessment of population structure
           and genetic diversity for morpho-physiological traits in guava ( Psidium
           guajava L.)
    • Authors: Dharamjit Kherwar; Kalidindi Usha; S. V. Amitha Mithra; Bhupinder Singh
      Abstract: Twenty-four SSR markers were utilized to evaluate the genetic variation across thirty-six guava varieties including wild species. The SSR markers revealed a polymorphism of 95.7% and a great range of diversity among the experimental guava germplasm. Eighty-one alleles were detected, in diversity analysis, with 2–7 alleles with a mean of 3.682 alleles per loci. The SSR loci showcased an allele frequency of 0.306 (mPgCIR251) to 0.861 (mPgCIR227) at a mean value 0.561. An average polymorphic value of 0.490 across was measured for all the 36 germplasm with the range of 0.234 in mPgCIR227 to 0.706 in mPgCIR03. The genetic diversity for SSRs varied between 0.248 (mPgCIR227) and 0.747 (mPgCIR03) with an average of 0.548. Clustering of germplasm distinctly separated pink and white flesh germplasm into two major groups. First three coordinates contributed towards 32.76% of the variation measured using principle coordinate analysis. Molecular variance (AMOVA) study showed 06 and 94% genetic variation among population and individual, respectively with five sub populations. This study provides valuable information for understanding the genetic variability in guava which can be exploited to develop varieties with better fruit yield and nutritional quality.
      PubDate: 2018-01-06
      DOI: 10.1007/s13562-017-0438-2
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Tel: +00 44 (0)131 4513762
Fax: +00 44 (0)131 4513327
Home (Search)
Subjects A-Z
Publishers A-Z
Your IP address:
About JournalTOCs
News (blog, publications)
JournalTOCs on Twitter   JournalTOCs on Facebook

JournalTOCs © 2009-