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BIOTECHNOLOGY (244 journals)                  1 2 | Last

Showing 1 - 200 of 244 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 17)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 9)
Advances in Regenerative Medicine     Open Access   (Followers: 3)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 11)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 33)
Amylase     Open Access  
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 8)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 45)
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 67)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Beitr?ge zur Tabakforschung International/Contributions to Tobacco Research     Open Access   (Followers: 3)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 4)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal   (Followers: 1)
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical Glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosensors Journal     Open Access  
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 8)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 34)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 160)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 13)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 2)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 17)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 41)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
BIOTIK : Jurnal Ilmiah Biologi Teknologi dan Kependidikan     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 17)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 13)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
DNA and RNA Nanotechnology     Open Access  
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription   (Followers: 1)
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticultural Biotechnology Research     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Indonesian Journal of Medicine     Open Access  
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 14)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 4)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
JMIR Biomedical Engineering     Open Access  
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of BioScience and Biotechnology     Open Access  
Journal of Biosecurity Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 63)
Journal of Biotechnology and Strategic Health Research     Open Access   (Followers: 1)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription   (Followers: 1)
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Ecobiotechnology     Open Access  
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 18)
Journal of Integrative Bioinformatics     Open Access  
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 13)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 13)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Meat Technology     Open Access  
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microbial Biotechnology     Open Access   (Followers: 10)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access   (Followers: 1)
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 2)

        1 2 | Last

Journal Cover
Journal of Plant Biochemistry and Biotechnology
Journal Prestige (SJR): 0.34
Citation Impact (citeScore): 1
Number of Followers: 4  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0971-7811 - ISSN (Online) 0974-1275
Published by Springer-Verlag Homepage  [2352 journals]
  • Improvement of blast resistance of the popular high-yielding, medium
           slender-grain type, bacterial blight resistant rice variety, Improved
           Samba Mahsuri by marker-assisted breeding
    • Authors: G. Rekha; V. Abhilash Kumar; B. C. Viraktamath; K. Pranathi; M. B. V. N. Kousik; B. Laxmi Prasanna; C. Backiyalakshmi; Pragya Sinha; R. K. Ravindra; S. Bhaskar; S. K. Hajira; C. H. Balachiranjeevi; K. Swapnil; R. Rambabu; G. Harika; E. Punniakotti; M. Anila; H. K. Mahadev; T. Dilip Kumar; A. Yugander; K. Chaitra; M. Praveen; K. R. Madhavi; M. S. Prasad; G. S. Laha; C. N. Neeraja; S. M. Balachandran; P. Senguttuvel; R. A. Fiyaz; J. Badri; A. Giri; L. V. Subba Rao; V. Ravindra Babu; R. M. Sundaram
      Abstract: Improved Samba Mahsuri (ISM) is a popular, high-yielding, bacterial blight resistant rice variety possessing medium-slender grain type. As ISM is highly susceptible to blast disease of rice, through the present study we have transferred two major blast resistance genes, Pi2 and Pi54 into the elite variety by marker-assisted backcross breeding. The two blast resistance genes were transferred to ISM through sets of backcrosses. In every backcross generation, PCR-based markers, specific for the blast resistance genes (Pi2 and Pi54) and bacterial blight resistance genes (Xa21, xa13 and xa5) were utilized for foreground selection, while a set of 144 parental polymorphic SSR markers were used for background selection and backcrossing was carried out until BC2 generation. A solitary BC2F1 plant possessing Pi2 or Pi54 along with Xa21, xa13 and xa5 and > 90% recovery of ISM genome was selected from the two sets of backcrosses were crossed and the intercross F1s (ICF1s) thus obtained were selfed to generate ICF2s. Homozygous ICF2 plants carrying all the five resistance genes were identified through markers and advanced through selfing till ICF5 generation by adopting pedigree method of selection. Three best lines at ICF5, possessing excellent resistance against bacterial blight and blast and closely resembling or superior to ISM in terms of grain quality: yield and agro-morphological traits have been identified and advanced for multi-location trials.
      PubDate: 2018-04-20
      DOI: 10.1007/s13562-018-0455-9
  • Development of immunodiagnostics for the detection of Grapevine leafroll -
           associated virus 3 (GLRaV-3) in grapevine using in vitro expression and
           purification of its coat protein gene
    • Authors: Sandeep Kumar; Priyanka Singh; Richa Rai; Virendra Kumar Baranwal
      Abstract: A previously cloned coat protein (CP) gene of Grapevine leafroll-associated virus 3 (GLRaV-3) from cultivar Cabernet Souvignon was over-expressed in Escherichia coli strain BL21 expression system as ~ 43 kDa fusion protein containing polyhistidine tag (6His) at its N terminal. The protein was purified from insoluble fraction and reacted positively in western blotting with commercial anti GLRaV-3 polyclonal antiserum (Bioreba, Switzerland) and hence, used as immunogen for the production of polyclonal antisera in New Zealand white rabbits. Polyclonal antiserum specific to GLRaV-3 detected the virus by double antibody sandwich enzyme linked immunosorbent assay using commercial alkaline phosphatase (ALP) conjugated globulin fraction (Bioreba, Switzerland) in GLRaV-3 positive grapevine samples. The immunoreactivity of the antiserum was confirmed through western blotting. The purified antiserum was conjugated with ALP. The primary antiserum along with ALP conjugate successfully detected the GLRaV-3 from the infected sample at 1:8000 and 1:10,000 dilutions, respectively. To the best of our knowledge, it is the first global study wherein the CP of GLRaV-3 was cloned in pET28a(+) expression vector having many advantages over the earlier used expression vectors. The cloned CP gene was expressed, purified and subjected to the production of immunoreagents. The developed immunoreagents will be useful for certification programmes as well as for large scale virus screening to produce GLRaV-3 free grapevines. The indigenously developed immunereagents will provide a cost-effective way of managing grapevine leafroll disease in Indian sub-continent.
      PubDate: 2018-04-04
      DOI: 10.1007/s13562-018-0451-0
  • Real-time PCR assay for rapid, efficient and accurate detection of
           Paramyrothecium roridum a leaf spot pathogen of Gossypium species
    • Authors: R. L. Chavhan; V. R. Hinge; U. S. Kadam; B. B. Kalbande; P. K. Chakrabarty
      Abstract: Here we report a highly sensitive real-time PCR (qPCR) assay to detect Paramyrothecium roridum from pure culture and infected samples of cotton plants. A specific set of primer pair pMyro F/R is designed to target the 185 bp ITS region of rDNA of Paramyrothecium roridum species and validated using qPCR. The fluorescence signals were detected above the baseline threshold from samples containing Paramyrothecium roridum DNA, whereas other samples did not produce any fluorescence or produced fluorescence which did not reach detection threshold values. A single dissociation peak of increased fluorescence was obtained for the specific primers at 92.2 °C melting temperature. The limit of detection using SYBR Green dye in this assay was up to 0.1 pg per µL of DNA from pure culture of P. roridum. The assay is accurate, sensitive, less laborious and time saving for detection of P. roridum in infected tissues of cotton.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0431-9
  • Arabidopsis thaliana DHS2 ( AT4G33510 ) gene promoter is highly wound
           responsive and requires a part of the first exon sequences for its
    • Authors: Ritesh Kumar Raipuria; Vajinder Kumar; Kadur Narayan Guruprasad; Shripad Ramachandra Bhat
      Abstract: Wound-responsive promoters are critical for developing the next generation of pest-tolerant transgenics, which would defend host by producing defensive factors at the site of attack. As shikimate pathway genes are known to be induced upon wounding, we evaluated the promoter of Arabidopsis thaliana DHS2 gene, which encodes for the first enzyme of this pathway. Transcription start site mapping showed DHS2 transcript has 89 nucleotide long 5′ UTR and RT-PCR revealed constitutive expression of DHS2 gene. Promoter analysis in transgenic A. thaliana using uidA reporter gene revealed that a part of the first exon of DHS2 is essential for promoter function. Histochemical GUS expression studies showed DHS2 expression in all green parts with the least expression in seedling roots and pollens. DHS2 promoter was found to be highly responsive to wounding giving 2–4 log fold up regulation within 10 min of wounding. Elevated expression level remained stable for 2–3 h and returned to normal after 24 h. In silico search identified wound responsive cis elements within the first exon of the DHS2 gene further underscoring the role of coding sequences in wound response and promoter activity. This is the first report demonstrating that coding sequence of a gene is an essential part of its promoter.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0430-x
  • Anti-VEGFR2 nanobody expression in lettuce using an infectious Turnip
           mosaic virus vector
    • Authors: Malihe Mirzaee; Mokhtar Jalali-Javaran; Ahmad Moieni; Sirous Zeinali; Mahdi Behdani; Masoud Shams-Bakhsh; Mostafa Modarresi
      Abstract: Angiogenesis plays an important role in tumor growth and metastasis of cancer. Vascular endothelial growth factor is the key regulator in stimulating angiogenesis. The VEGF activity is mediated by binding to its cell-surface receptors, mainly VEGFR2. Therefore, inhibition of the VEGF/VEGFR2 interaction by antibodies is investigated as a therapeutic strategy in cancer therapy. Here, we describe transient expression of an anti-VEGFR2 nanobody (3VGR19) by a viral vector based on Turnip mosaic virus in lettuce (Lactuca sativa L.). RT-PCR analysis demonstrated the 3VGR19 transcript expression. Western blot analysis showed the 3VGR19 protein expression with an expected molecular mass of ~15 kDa and based on the ELISA results, the expression level of 3VGR19 was 8 μg/g of leaf fresh weight. Taken together, recombinant 3VGR19 could be efficiently expressed in lettuce leaves and TuMV-based expression system would be an appropriate platform for production of recombinant proteins in lettuce plants.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0425-7
  • Development and validation of multiplex-PCR assay for simultaneous
           detection of rare alleles of crtRB1 and lcyE governing higher accumulation
           of provitamin A in maize
    • Authors: Rajkumar Uttamrao Zunjare; Rashmi Chhabra; Firoz Hossain; Vignesh Muthusamy; Aanchal Baveja; Hari Shanker Gupta
      Abstract: Vitamin A deficiency is a widely prevalent health disorder among millions of people worldwide. Introgression of crtRB1 and lcyE favourable alleles that enhance concentration of provitamin A in maize endosperm have been employed in maize biofortification programmes. To make marker-assisted selection (MAS) more effective, we have developed rapid and convenient multiplex-polymerase chain reaction (PCR) assay to simultaneously discover the allelic combinations among the segregants. Validation of the multiplex assay was done in two backcross-derived populations developed using elite inbreds viz., HKI193-1 and HKI193-2 carrying unfavourable alleles of crtRB1 (296 bp) and lcyE (300 bp) and HarvestPlus inbreds viz., HP704-22 and HP704-23 possessing favourable alleles of crtRB1 (543 bp) and lcyE (650 bp). We also standardized the uniplex-PCR assays for both the genes that gave robust and reproducible results in sub-tropical populations. Gel profiles of BC1F1, BC2F1 and BC2F2 revealed that these assays identified the backcross progenies homo-or hetero-zygous for the favourable- or unfavourable-alleles. Multiplex-PCR assay also precisely confirmed the results of individual uniplex assays in different backcross generations. Cost and time analyses showed that multiplex-PCR assay has potential to save 41% of cost, and 50% of time compared to two uniplex assays in a MAS programme. It has also saved 50% of the manpower. The multiplex assay possesses significant advantage over uniplex assays and enhances the efficiency of selection. This is the first report of development and validation of multiplex-PCR assay of crtRB1 and lcyE for utilization in maize biofortification programme.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0432-8
  • Physiological, biochemical, antioxidant and growth characterizations of
           gibberellin and paclobutrazol-treated sweet leaf ( Stevia rebaudiana B.)
    • Authors: Shokoofeh Hajihashemi
      Abstract: A greenhouse experiment was designed to study the responses of Stevia rebaudiana herb to paclobutrazol (PBZ) and gibberellin (GA) treatments. GA and PBZ treatments caused no significant impact on photosynthesis pigments while they increased carbohydrates, amino acids and protein metabolites. Stevia showed a potent antioxidant activity through scavenging DPPH, NO·; O 2 ·− and OH· radicals which was highlighted in GA and PBZ treatments. The enzymatic and non-enzymatic antioxidant system of Stevia plant showed a significant increase in response to PBZ and GA treatments. PBZ treatment decreased plant growth while GA treatment had no significant effect on it. Collectively, both GA and PBZ treatments effectively increased metabolites and antioxidant property of Stevia herb.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0428-4
  • Identification of functional flavonol synthase genes from fragrant wild
           cyclamen ( Cyclamen purpurascens )
    • Authors: Yusuke Akita; Satoshi Kitamura; Riho Mikami; Hiroshi Ishizaka
      Abstract: Cyclamen purpurascens is considered suitable for horticultural breeding of cyclamens because it has an attractive fragrance that is not found in other wild species. To improve the commercial value of cyclamen flowers, this fragrance has been introduced into ornamental cultivars. However, variation in flower color is somewhat limited in these cultivars, and therefore understanding the genetic networks of flower coloration in C. purpurascens is required. We previously isolated DNA fragments of anthocyanin biosynthetic genes from C. purpurascens, broadening our understanding of the biosynthetic pathway of flavonols, which are co-pigments in flower coloration. In this study, we isolated complete open reading frames of flavonol synthase genes from C. purpurascens (CpurFLS1 and CpurFLS2) and analyzed the in planta functions of the genes by molecular complementation assay using the fls mutant of Arabidopsis thaliana. Expression patterns in several organs of C. purpurascens were also determined. The results strongly suggest that the CpurFLS genes participate in flavonol synthesis. We discuss the involvement of these two FLSs in flower coloration in C. purpurascens.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0423-9
  • Cloning and molecular characterization of a ferulate-5-hydroxylase gene
           from water chestnuts ( Trapa bicornis Osbeck.)
    • Authors: Fulin Zhang; Fangqin Wang; Dahui Li
      Abstract: Ferulate 5-hydroxylase (F5H), a cytochrome P450 monooxygenase, has been indicated to function in the monolignol biosynthesis of sinapyl alcohol, namely the precursor of syringyl lignin during phenylpropanoid metabolism. In the present study, a full-length cDNA clone encoding F5H, designated as TbF5H1, was cloned from water chestnuts (Trapa bicornis Osbeck.) using RT-PCR and RACE. Sequence analysis of TbF5H1 protein revealed the presence of highly conserved motifs shard by P450 s, such as heme binding cysteine, oxygen binding I-helix, and E-R-R triade. Subsequently, TbF5H1 was expressed in bacterial system as a recombinant protein, which was used to ascertain kinetic parameters of the protein with the Km and Vmax values of 2.40 μM and 3.35 pkat mg−1, respectively. Together with high identity with other plant F5Hs based amino acid sequences, this result suggested that TbF5H1 had a biological function similar to its homologues in S lignin synthesis. Western blotting analysis using anti-TbF5H1 serum towards total proteins extracted from roots, stems, leaves, and flowers, detected a protein with a molecular weight of about 57 kDa. Quantitative real-time PCR analysis revealed the transcript level of TbF5H1 was higher in leaves than in other tested tissues. Interestingly, TbF5H1 was differentially expressed in response to various stresses, including salt (NaCl), hydrogen peroxide (H2O2) and heavy metal (lead), as well as elicitation by methyl ester jasmonate (MeJA). The expression of TbF5H1 was not significantly altered under NaCl or lead stress conditions, whereas a significant increase in TbF5H1 expression was detected after elicitation of H2O2 and MeJA, respectively. Moreover, there existed a fine coordination between expression of TbF5H1 and contents of certain nonenzymatic antioxidants. These results indicated that integrated with some redox components, TbF5H1 gene should contribute to the acclimation of T. bicornis to oxidative stress, which is modulated by MeJA.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0422-x
  • The maize brevis plant1 is a type II inositol polyphosphate 5-phosphatase
    • Authors: Yu Chen; Qinan Cai; Shuiyuan Hao
      Abstract: The genes associated with the dwarf phenotype have been utilized in crop breeding to prevent lodging and stem breakage. Brevis plant1 (Bv1), encoding a putative inositol polyphosphate 5-phosphatase (5PTase), has been associated with stem elongation in maize (Zea mays L.); however, the enzymatic activity of BV1 has not been experimentally characterized. In this study, the phosphatase activity of BV1 was verified with biochemical assays. BV1 demonstrated type II 5PTase activity capable of hydrolyzing both inositol polyphosphates and phosphoinositides. Similar to other type II 5PTases that share similar sequences and common domain architecture with BV1, the enzymatic activity of BV1 is sensitive to changes in Mg2+ and pH. 367G-R and 565S-L are two mutations in Bv1 that have been associated with the dwarfing phenotype in maize. To characterize the dwarfing mechanism, mutant BV1 proteins were expressed in vitro and assayed for phosphatase activity. The results showed that both mutations significantly reduced the enzymatic activity of BV1, but neither altered its substrate specificity and dependence on Mg2+ and pH. This biochemical verification of BV1 as a type II 5PTase is important as it sheds light on the molecular basis of deficient BV1-mediated dwarfing phenotype.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0433-7
  • Genome-wide identification and abiotic stress responses of DGK gene family
           in maize
    • Authors: Yingnan Gu; Changjiang Zhao; Lin He; Bowei Yan; Jiejing Dong; Zuotong Li; Kejun Yang; Jingyu Xu
      Abstract: Diacylglycerol kinase (DGK) is a kind of phosphokinase that catalyzes the formation of signaling molecule phosphatidic acid. In this study, seven maize (Zea mays) DGK gene family members were identified by an exploration of maize genome via multiple online databases, and designated as ZmDGK1-7, respectively. The proteins encoded by ZmDGKs ranged from 487 to 716 amino acids, and had a molecular weight (MWs) between 54.6 and 80.2 kDa. Phylogenetic analysis revealed that ZmDGKs grouped into three clusters as described for known plant DGK families: Cluster I was composed of three maize DGKs, ZmDGK1, ZmDGK4 and ZmDGK5, cluster II contained ZmDGK6, and the isoforms ZmDGK2, ZmDGK3 and ZmDGK7 fell into cluster III. ZmDGK proteins featured the typical functional domains, while all seven ZmDGKs have a conserved catalytic domain DGKc, only the cluster I ZmDGKs have the DAG/PE binding domain. Most ZmDGK genes showed ubiquitous expression profiles at various developmental stages, while a high relative expression was observed at the tasseling stage. ZmDGK genes exhibited differential expression patterns in response to abiotic stresses including cold, salinity and drought, and all ZmDGK genes were found obviously up-regulated by cold. The distinct roles of ZmDGKs in cold response was also supported by the finding that an accumulation of DGK products–PA under low temperature. This study will help to better understand the roles of DGKs in the development and abiotic stress responses in major crops.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0424-8
  • Cloning, antibody production, expression and cellular localization of
           universal stress protein gene ( USP1 -GFP) in transgenic cotton
    • Authors: Sameera Hassan; Tahir Rehman Samiullah; Mahmood ur Rahman Ansari; Bushra Rashid; Tayyab Husnain
      Abstract: This study was aimed to clone the universal stress protein (GUSP1) gene isolated from Gossypium arboreum in E. coli expression vector pET30(a) and to raise the specific antibody in rabbit to devise a system that could be used for localization and expression of this gene under drought stress. The amplification of GUSP1 transgene revealed a fragment of 500 bp via PCR in genomic DNA of transgenic cotton plants and expression was confirmed through ELISA and Western blot by using the GUSP1 specific polyclonal antibodies. ELISA showed the expression of GUSP1 protein in roots, stem and leaves of transgenic plants at seedling, vegetative and mature plant developmental stages. Total protein isolated from drought stressed transgenic plants revealed a fragment of 47 kDa (GUSP1-GFP fusion protein) in Western blot which confirmed the expression of transgene. Confocal microscopy detected the GFP fluorescence as localization of GUSP1 in the midrib, guard cells of stomata, trichome and globular trichome of intact leaf of transgenic plants. The co-localization was observed within cytoplasm, palisade, spongy mesophyll, guard cells of stomata, vascular bundle, trichome and globular trichome of transgenic plants by using the GUSP1 specific primary antibodies and Alexa fluor conjugated secondary antibodies. This study of GUSP1 gene will advance the mechanism of abiotic stress tolerance in plants.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0427-5
  • Isolation and characterization of a 3-hydroxy-3-methylglutaryl coenzyme A
           reductase 2 promoter from Salvia miltiorrhiza
    • Authors: Piotr Szymczyk; Renata Grąbkowska; Ewa Skała; Marta Żebrowska; Ewa Balcerczak; Agnieszka Jeleń
      Abstract: The aim of the present study was to isolate and characterize the 5′ regulatory region of Salvia miltiorrhiza 3-hydroxy-3-methylglutaryl coenzyme A reductase 2 gene. The entire fragment is 2696 bp long and consists of the promoter, 5′UTR and 85 nucleotide 5′ fragments of the CDS region. The results of in silico bioinformatic tests indicate that the promoter region contains repetitions of many potential cis-active elements serving as the recognition sites for transcription factors. Data obtained from the in silico tests are verified by co-expression studies based on A. thaliana microarray data to make them more probably to occur. The bioinformatic analysis indicated no tandem repeats or CpNpG islands in the promoter. However, potential target sites for miRNA 156 and miRNA 159 were found in the 5′ UTR segment. In addition, two possible polymorphic sites, A2719G and A2744C, were found in the CDS region. Finally, the activity of isolated fragment was evaluated experimentally by quantitative RT–PCR. The promoter activity of the isolated 2696 bp HMGR2 gene fragment was confirmed by RT–PCR studies of in vitro cultured, transformed S. miltiorrhiza plants. Analysis of the RT–PCR results revealed temporal changes in the promoter activity occurring in response to treatment by five abiotic factors: auxin, gibberellin, salicylic acid, abscisic acid and 100 mM NaCl.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0434-6
  • Biology of B. sorokiniana (syn. Cochliobolus sativus ) in genomics era
    • Authors: Pushpendra K. Gupta; Neeraj K. Vasistha; Rashmi Aggarwal; Arun K. Joshi
      Abstract: Bipolaris sorokiniana (Sacc.) Shoemaker is a hemi-biotrophic fungal pathogen, which is an anamorph (teleomorph Cochlibolus sativus). It causes spot blotch, root rot and leaf spot diseases in a number of cereals including wheat, barley and other small grain cereals. In the genomics era, the fungus has been subjected to a variety of studies using molecular approaches. Correct chromosome number was determined and molecular karyotypes were prepared using contour-clamped homogeneous electric field. Molecular maps were prepared using markers like RFLPs, SSRs, RAPDs and SNPs. For this purpose, segregating progenies derived from crosses between diverse isolates of the pathogen were used. Whole genome sequencing (WGS) data was collected not only for B. sorokiniana isolates, but also for several species of Cochliobolus. Genes involved in secondary metabolism and virulence were identified from genome sequences. The WGS data has also been utilized for comparative genomics giving useful information about evolutionary trends. A brief account of this information is presented in this review.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0426-6
  • Extraction, purification and characterization of a novel cysteine protease
           from the latex of plant Vallaris solanacea
    • Authors: Silpa Somavarapu; Sandeep Vemula; I. Bhaskar Reddy
      Abstract: Plant proteases with excellent catalytical properties perform many functions in biological systems. A novel plant protease Vallaris solanacea, was identified. Its proteolytic activity was screened using the substrate casein. This protein activity was specifically inhibited by p-chloromercuribenzoate, which showed that it is a cysteine protease. Preliminary investigations such as pH effect and temperature dependence on the caseinolytic activity of crude protease were done. Stability towards temperature and pH were also evaluated. The activity curves drawn in relation to pH, temperature and stability suggested the presence of one protease in the latex of Vallaris solanacea. In the present study, separation and purification of the latex cysteine protease solanain from Vallaris solanacea to a state of near homogeneity was also done using ion exchange and size exclusion chromatography. SDS PAGE was used to determine molecular weight of the solanain (28–29 kDa). The molecular weight was confirmed as 28.9 kDa using MALDI-TOF. Purified protease was named solanain and it was further characterized. An internal tryptic fragment was identified by MALDI-TOF, and this peptide showed a homology (66% sequence similarity) with target sequence of cysteine endopeptidase from Ricinus communis.
      PubDate: 2018-04-01
      DOI: 10.1007/s13562-017-0429-3
  • Analysis of activity driven by upstream regulatory modules (URM) of
           tapetum specific genes TA29 and A9 at ectopic locations in tobacco
    • Authors: Preeti Apurve Sharma; Neetu Verma; Pradeep Kumar Burma
      Abstract: TA29 and A9 are genes from Nicotiana tabacum and Arabidopsis thaliana respectively, which express in a tapetum specific manner. The upstream regulatory modules (URMs; i.e. the promoter and the 5′UTR) of these genes have been used in development of male sterile and restorer lines expressing the barnase and barstar genes for hybrid seed production. While initial studies show that these URMs drive the expression in a tapetum specific manner, there are no recordings of unintended (leaky) expression driven by these URMs at ectopic locations due to position effect in developed transgenic lines. The information on leaky expression driven by tissue specific URMs is important for their use in developing transgenic plants. The present study records the leaky activity of both these URMs in transgenic tobacco lines using β-glucuronidase as a reporter gene. Leaky activity was observed in about one-fourth of the lines developed with TA29. Most interestingly in these lines, the leaky expression of the reporter gene was observed to be restricted to the meristematic tip region of the roots and at the leaf gap from where leaf trace diverges from stem bundles. Such a restricted and unique pattern of leaky activity of a tissue specific promoter or a URM has never been reported before, including the URM of the A9 gene analyzed in the present study. This observation suggests the presence of cryptic cis-elements within the URM of TA29 gene that can possibly activate it in meristematic tissue when integrated at certain ectopic locations. The URM of the A9 gene was also observed to show leaky activity. However, there was no unique pattern as observed with that of TA29. Further, in the study we also show that while the smaller (290 bp) length of TA29 URM can be used to drive the expression of barnase gene to develop male sterile lines, it adversely affects the regeneration of transgenic tobacco lines due to leaky expression. This adverse effect is significantly reduced when the full length (1.5 kb) URM of the TA29 gene is used.
      PubDate: 2018-03-28
      DOI: 10.1007/s13562-018-0453-y
  • Comparative characterization of small RNAs derived from an emaravirus and
           a geminivirus infecting pigeonpea
    • Authors: Basavaprabhu L. Patil; Deepika Arora
      Abstract: High throughput sequencing technologies, supported by bioinformatics tools are employed to retrieve small RNA sequence information derived from the nucleic acids of plant infecting viruses. In addition to characterization of the small RNAs to understand the biology of the virus, the small RNA sequence can be assembled to reconstitute viral genome sequence. For the first time the semiconductor based Ion Proton sequencing technology is used to sequence the small RNAs from pigeonpea (Cajanus cajan) plants infected by two distinct viruses with RNA and DNA as their genomes. The reconstitution of the viral genome sequence revealed that the pigeonpea plant from Kalaburagi (erstwhile Gulbarga, Karnataka state) was infected by an emaravirus species Pigeonpea sterility mosaic emaravirus 1 (PPSMV-1) and another plant from New Delhi was infected by a begomovirus species Mungbean yellow mosaic India virus (MYMIV). Characterization and comparison of small RNA sequences derived from both the viruses showed vast differences in their pattern of accumulation and their size classes. In the case of PPSMV-1, the 21 nt sized siRNAs accumulated at far greater levels followed by 22 and 24 nt siRNAs. Whereas in MYMIV, the proportion of accumulation of each size class of siRNAs was similar. Further the distribution of small RNAs across the genomes of PPSMV-1 and MYMIV was mapped and the density of small RNA accumulation showed a positive correlation with the GC content of viral sequence.
      PubDate: 2018-03-03
      DOI: 10.1007/s13562-018-0447-9
  • Genetic diversity changes in Indian lentils over the times
    • Authors: Jitendra Kumar; Sunanda Gupta; Sonali Dubey; Priyanka Gupta; Debjyoti Sen Gupta; N P Singh
      Abstract: The genetic diversity of 96 genotypes of lentil comprising 34 cultivars, 46 advanced breeding lines, and 16 germplasm lines were studied using 260 SSR markers. These markers generated a total of 749 alleles. The alleles/locus ranged from 2 to 16 with an average value of 2.87. Polymorphic information content varied from 0.02 to 0.91 with a mean of 0.30. Major allelic frequency ranged from 0.14 to 0.99 with a mean of 0.77. Studied genotypes were clustered into two groups according to their breeding history. Advanced breeding lines derived from exotic lines were clustered in one group, while another group accommodated most of the cultivars and advanced breeding lines with common cultivars in parentage. The germplasm lines were sub-clustered within first group. Cumulatively, first three principal components contributed 21.2% to the total variability. Advanced breeding lines showed higher number of alleles/locus and gene diversity (He) than other sets of genetic materials. In present study, no significant differences were observed between cultivars developed in different decadal groups for both NA and He. Moreover, genetic diversity changes between small and large seeded lentil cultivars were also found non-significant in this study. These findings showed that the use of alien genes can help to diversify active gene pool for developing improved new cultivars in lentil.
      PubDate: 2018-03-03
      DOI: 10.1007/s13562-018-0450-1
  • A rice bZIP transcription factor, OsbZIP16 , regulates abiotic stress
           tolerance when over-expressed in Arabidopsis
    • Authors: Agni Shekhar Pandey; Eshan Sharma; Nitin Jain; Brinderjit Singh; Naini Burman; Jitendra P. Khurana
      Abstract: Plants exposed to adverse environmental conditions are invariably compromised in their growth and development. The bZIP class of transcription factors (TF) form a large group among stress signalling components that regulate plant responses towards stress. We identified bZIP TF encoding genes that are expressed differentially in indica rice under stress and here we functionally characterize one such gene, OsbZIP16. Although, OsbZIP16 forms a clade with its orthologous monocot protein sequences, we find in our study that it can impart tolerance to abiotic stress in Arabidopsis. OsbZIP16 is expressed strongly upon dehydration, salt and ABA treatment in Oryza sativa cv. IR64 seedlings. It localizes in the cell nucleus and the gene product is capable of transcriptional activation, thus providing evidence for its capability as a functional TF. Upon overexpression in Arabidopsis, OsbZIP16ox plants show wild type morphology, however, these plants showed tolerance when subjected to drought stress at vegetative stage and set healthy seeds on recovery. The OsbZIP16ox seedlings showed reduced sensitivity to mannitol, ABA and sodium chloride during germination and also reduced ROS accumulation upon H2O2 exposure. Thus, OsbZIP16 regulates abiotic stress responses and is also a good candidate gene that can be utilised to impart tolerance in plants under water deficit conditions.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0448-8
  • Isolation and expression analysis of eight MADS - box genes in peach (
           Prunus persica var. nectarina ‘Luxing’)
    • Authors: Hui-feng Li; Qing-long Dong; Hou-zhen Jia; Gui-xiang Li; Kun Ran
      Abstract: The MADS-box transcription factor (TF) plays a crucial regulatory role in plant vegetative growth, flower and fruit development. Eight MADS-box genes (designated as PpMADS15, 16, 17, 26, 27, 36, 37, 38; GenBank accession nos. KU559581, KU559582, KU559583, KU559592, KU559593, KU559602, KU559603, KU559604, respectively) were isolated from ‘Luxing’ (Prunus persica var. nectarina ‘Luxing’) peach by homologous comparison and RT-PCR, which contained open reading frames (ORF) of 597, 750, 1062, 615, 699, 1 107, 678 and 564 bp, respectively. The results of phylogenetic analysis revealed that PpMADS15 belonged to the AG subgroup, PpMADS16 to the SEP subgroup, PpMADS17 to the MIKC* group, PpMADS26, 27, and 38 to the Mα group, and PpMADS36 and 37 to the Mγ group. The results of the prediction for subcellular localization showed that eight PpMADS proteins were located in the nucleus. The results of promoter analysis indicated that there were multiple putative cis-acting elements that were involved in responsiveness to the following variables: light, defense and stress, low-temperature, heat stress, wound, fungal elicitor, anaerobic induction, MeJA, gibberellin, ABA, auxin, and SA. RT-PCR results showed that PpMADS15 was expressed in leaves, stems, roots, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS16 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS17 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development (except for 30 d). All members in the Mα and Mγ subgroups were expressed in roots, stems, leaves, sepals, ovaries, stamens, petals and during flower development, but PpMADS27 was expressed only during fruit development. These results suggested that eight PpMADS genes played a crucial regulatory role in vegetative growth, flower and fruit development of peaches.
      PubDate: 2018-03-02
      DOI: 10.1007/s13562-018-0452-z
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