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BIOTECHNOLOGY (244 journals)                  1 2 | Last

Showing 1 - 200 of 244 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 17)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 9)
Advances in Regenerative Medicine     Open Access   (Followers: 3)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 11)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 33)
Amylase     Open Access  
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 8)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 45)
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 67)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Beitr?ge zur Tabakforschung International/Contributions to Tobacco Research     Open Access   (Followers: 3)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 4)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal   (Followers: 1)
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical Glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosensors Journal     Open Access  
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 8)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 34)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 159)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 13)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 2)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 17)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 41)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
BIOTIK : Jurnal Ilmiah Biologi Teknologi dan Kependidikan     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 17)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 13)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
DNA and RNA Nanotechnology     Open Access  
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription   (Followers: 1)
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticultural Biotechnology Research     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Indonesian Journal of Medicine     Open Access  
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 14)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 4)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
JMIR Biomedical Engineering     Open Access  
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of BioScience and Biotechnology     Open Access  
Journal of Biosecurity Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 63)
Journal of Biotechnology and Strategic Health Research     Open Access   (Followers: 1)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription   (Followers: 1)
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Ecobiotechnology     Open Access  
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 18)
Journal of Integrative Bioinformatics     Open Access  
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 13)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 13)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Meat Technology     Open Access  
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microbial Biotechnology     Open Access   (Followers: 10)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access   (Followers: 1)
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 2)

        1 2 | Last

Journal Cover
Horticulture, Environment, and Biotechnology
Journal Prestige (SJR): 0.441
Citation Impact (citeScore): 1
Number of Followers: 11  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 2211-3452 - ISSN (Online) 2211-3460
Published by Springer-Verlag Homepage  [2352 journals]
  • Molecular characterization of pomegranate ( Punica granatum L.) accessions
           from Fars Province of Iran using microsatellite markers
    • Authors: Abdolkarim Zarei; Amir Sahraroo
      Pages: 239 - 249
      Abstract: Pomegranate is a long-cultivated fruit tree believed to have originated in Iran. In the present study, 16 preselected nuclear microsatellite markers, or simple sequence repeats (SSRs), were analyzed in 50 pomegranate (Punica granatum L.) accessions from five regions in Fars province of Iran. Each SSR loci was polymorphic and produced 48 fragments in the studied samples. The mean expected and observed heterozygosity of the 16 SSR loci was 0.33 and 0.48, respectively. The polymorphic information content ranged from 0.18 to 0.58 with an average of 0.41. There were some differences regarding diversity indices among populations, and several private alleles were detected in different populations, indicating the importance of these accessions for genetic conservation. Cluster analysis using SSR data grouped genotypes largely based on their geographical origins. Analysis of molecular variance showed that most of the genetic variation was among populations. Genetic synonymy was observed in some pomegranate accessions located across geographical regions. A relatively high level of genetic admixture was found among accessions from different regions, suggesting that there is a high level of genetic exchange between individual genotypes. This work assesses the genetic diversity and population structure of pomegranates in Fars province, which assists in future conservation and breeding programs.
      PubDate: 2018-04-01
      DOI: 10.1007/s13580-018-0019-x
      Issue No: Vol. 59, No. 2 (2018)
  • Construction of genetic linkage maps of ‘Fina Sodea’ clementine (
           Citrus clementina ) and Byungkyul ( C. platymamma ), a Korean landrace,
           based on RAPD and SSR markers
    • Authors: Yali Chang; Ho Bang Kim; Eun-Ui Oh; Kyunguk Yi; Kwan Jeong Song
      Pages: 263 - 274
      Abstract: In this study, genetic linkage maps were constructed for ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma) using 152 F1 clones as a mapping population. Reference-guided whole-genome sequencing, using next-generation sequencing technology, along with 82 randomly amplified polymorphic DNA markers and seven genomic simple-sequence repeat (gSSR) loci, mined 41 polymorphic gSSR loci from the direct comparative analysis. The generated linkage map for ‘Fina Sodea’ clementine contained 22 markers distributed into 10 linkage groups, with a total length of 417.7 cM and an average interval of 19.0 cM. The map for Byungkyul contained 57 markers distributed into 10 linkage groups, with a total length of 684.9 cM and a smaller average interval of 12.0 cM. Comparison of shared gSSR markers between the two maps identified collinearities, such as linkage groups B3 and C7 and groups B9 and C7. The newly developed gSSR markers greatly enrich the limited amount of gSSR markers in Citrus, providing markers that can be useful for comparative mapping and other genetic studies.
      PubDate: 2018-04-01
      DOI: 10.1007/s13580-018-0021-3
      Issue No: Vol. 59, No. 2 (2018)
  • The effects of 24-epibrassinolide corm priming and foliar spray on
           morphological, biochemical, and postharvest traits of sword lily
    • Authors: Samaneh Mollaei; Homayoun Farahmand; Iraj Tavassolian
      Abstract: Gladiolus, or sword lily, is one of the most important cut flowers worldwide. Enhancement of its traits is of paramount importance to the flower industry. The application of 24-epibrassinolide (EBR) by corm priming and plant foliar spray to improve morphological, biochemical and postharvest characteristics of Gladiolus grandiflorus cv. Oscar were investigated. Concentrations of 0.5, 1, and 2 µM EBR as well as distillated water as a control were used for both corm priming and foliar spray treatments. Results showed that the highest levels of corm sprouting and flower spike emergence were obtained at 1 µM EBR. Floret numbers, flower spike fresh and dry weight, and vase life showed the highest values at 1 µM EBR combined treatments. The combination of corm priming at 2 µM and foliar spay at 1 µM EBR, showed the highest effect on malondialdehyde reduction. The highest activities of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase were obtained in combination of 1 µM EBR corm priming and 1 µM EBR foliar spray. EBR treatments prolonged vase life from 8 to 14 days and significantly improved gladiolus morphological and biochemical traits.
      PubDate: 2018-04-25
      DOI: 10.1007/s13580-018-0033-z
  • Comparison of the activities of photosystem II of four table grapevine
           cultivars during high-temperature stress
    • Authors: Qian Zha; Xiaojun Xi; Aili Jiang; Yihua Tian
      Abstract: High-temperature stress influences the growth and development of grapevines, and plant responses to this stress vary between laboratory and natural conditions. In the present study, the responses to high-temperature stress in four grapevine cultivars (‘Summer Black’, ‘Zuijinxiang’, ‘Hupei1#’, and ‘Shenfeng’) were studied by comparing chlorophyll a fluorescence and the levels of heat-shock protein 21 (HSP21) after exposure to control (35 °C in controlled laboratory conditions or at 37 °C in a greenhouse with naturally fluctuating temperatures) and high-temperature stress treatments (45 °C in laboratory conditions or 42 °C in the field conditions). Leaf water loss in ‘Summer Black’ was less than that in the three other cultivars after treatment at 45 °C. Some parameters (ΨEo, Wk, RCQA, and Mo) of photosystem II (PSII) activity were altered in ‘Shenfeng’, ‘Zuijinxiang’, and ‘Hupei1#’, but were unaltered in ‘Summer Black’ after treatment at 45 °C. Other parameters (maximum photochemical efficiency (Fv/Fm), performance index on absorption basis (PIABS), ΦPo, ΦEo, and HSP21 levels) were altered in ‘Summer Black’, but to a lesser extent than in the three other cultivars under high-temperature stress. Unlike ‘Summer Black’, extreme injury owing to leaf water loss to ‘Shenfeng’, ‘Zuijinxiang’, and ‘Hupei1#’ could be explained by disruption of PSII activity. Furthermore, there were the observed differences in PSII activity between in laboratory and field conditions, which could be considered as the mechanisms for high-temperature acclimation and other environment factors.
      PubDate: 2018-04-25
      DOI: 10.1007/s13580-018-0041-z
  • Different vegetative growth stages of Kimchi cabbage ( Brassica rapa L.)
           exhibit specific glucosinolate composition and content
    • Authors: Byeong Wook Jeon; Man-Ho Oh; Eun Ok Kim; Hyoung Seok Kim; Won Byoung Chae
      Abstract: This study aimed to simultaneously investigate the changes in growth characteristics and glucosinolate (GL) content during different growth stages in Kimchi cabbage. Two Kimchi cabbage cultivars ‘Chuweol’ and ‘HwiparamGold’ were grown in the field and plant characteristics such as leaf length, number and fresh weight, and GL contents were measured weekly from 2 to 9 weeks after transplanting (WAT). The only significant difference between the two cultivars for either plant growth or GL content was observed for GL contents during 3 and 7 WAT. Leaf length increased until 4 WAT and then remained unchanged, exhibiting logarithmic growth. The fresh weight and number of leaves increased linearly until 9 WAT. Five GLs (two aliphatic GLs: progoitrin and gluconapin, two indole GSLs: glucobrassicin and neoglucobrassicin, and one aromatic GL: gluconasturtiin) of the nine GLs investigated in this study (glucoiberin, progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, gluconasturtiin, 4-methoxy glucobrassicin and neoglucobrassin) were detected in the two cultivars. The contents of these five GLs were similar in the two cultivars during 2 WAT, but gluconapin and gluconasturtiin increased more dramatically than the others. The increasing pattern of total GL more closely resembled the leaf growth pattern than the fresh weight. Our results suggest that the change in total GL content positively correlates to leaf length, and the increase in total GL content is attributed to the increase in the amount of gluconasturtiin and gluconapin during the autumn growing season.
      PubDate: 2018-04-23
      DOI: 10.1007/s13580-018-0040-0
  • De novo transcriptome analysis of an albino mutant Pasphiopedilum pacific
           shamrock reveals reduced expression of genes related to chloroplast
           biosynthesis and division
    • Authors: Han Li; Hua Cao; Rong-pei Yu; Zhen Miao; Ji-hua Wang; Su-Ping Qu; Qiang Yuan; Shen-chong Li
      Abstract: Paphiopedilum pacific shamrock is an orchid with high ornamental value. Understanding the mechanisms responsible for leaf color in albino mutants is important for ornamental development and breeding. In this study, we compared the leaf photosynthetic pigment content and transcriptome of albino mutants ppa01 and wild-type P. pacific shamrock. Photosynthetic pigment in mutants was less than 2% of the wild type and chl a/b was 60% less than the wild type. Transcriptome sequencing yielded 6.27 Gb and 5.67 Gb clean data from the mutant and wild-type leaves, respectively. De novo assembly yielded 104,763 unigenes with 15,400 greater than 1 kb in length. In unigene expression analysis, 3170 differentially expressed genes (DEGs) were identified with 2231 (70.38% of total DEGs) down-regulated. Results from GO and KEGG enrichment analysis, KEGG pathway analysis and qPCR suggest that the reduction of chloroplast biosynthesis and division in the mutant was due to low expression levels of ppGLK1 and ppFtsz. Mutants were associated with fewer chloroplasts in leaf cells, abnormal chloroplast structures, impaired chlorophyll biosynthesis, and thus reduced total chlorophyll and carotenoid contents. Furthermore, down-regulated expression of ppNYC1 reduced transformation of chlorophyll b into chlorophyll a, leading to a chl a/b decline. The research will guide future studies of leaf pigment mutations and the breeding of P. pacific shamrock.
      PubDate: 2018-04-23
      DOI: 10.1007/s13580-018-0037-8
  • Effects of biochar mixtures with pine-bark based substrates on growth and
           development of horticultural crops
    • Authors: Hyun-Sug Choi; Yan Zhao; Haijie Dou; Xiaoya Cai; Mengmeng Gu; Fei Yu
      Abstract: This study investigates the potential of using biochar as a substrate component to replace pine bark (PB) to produce horticultural crops in containers. Biochar was incorporated in PB at 0, 20, 40, 60, 80 and 100% (by volume) to grow chrysanthemum, tomato, lettuce and basil plants in containers. The responses of plant growth to the percentage of biochar combined in PB mixtures varied among the different crops. Chrysanthemum plants grown in PB mixtures with 80% and 100% biochar had significant higher shoot fresh weight (FW) and dry weight (DW) than plants grown in PB alone, while the same treatments resulted in reduced shoot FW, DW, growth index measured at 19 and 33 days after transplanting and root rating of tomato plants. Growing lettuce plants in 100% biochar reduced the FW of the second crop compared to plants grown in PB substrates supplemented with 0, 20, 40, 60, and 80% biochar. For basil, although biochar alone caused lower root rating, it did not adversely influence the fresh basil yield. Generally, no negative effect on plant growth was observed in PB mixes with biochar as high as 60%, which is probably the outcome of similar physical properties of the biochar to the commonly used PB.
      PubDate: 2018-04-20
      DOI: 10.1007/s13580-018-0035-x
  • Allelic variation in Brassica oleracea CIRCADIAN CLOCK ASSOCIATED 1 (
           BoCCA1 ) is associated with freezing tolerance
    • Authors: Hayoung Song; Hankuil Yi; Ching-Tack Han; Jong-In Park; Yoonkang Hur
      Abstract: Freezing tolerance is an important horticultural trait in cabbage (Brassica oleracea). Molecular markers for freezing tolerance are needed for marker-assisted breeding of freezing-tolerant cabbage plants. To develop gene-based molecular markers for freezing-tolerance in cabbage, we focused on CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), a core circadian clock component that affects metabolic pathways and confers cold tolerance by upregulating C-repeat binding factor (CBF) pathway genes. We cloned and analyzed CCA1 genes (BoCCA1s) from seven inbred lines and one cultivar of B. oleracea ssp. capitata. Two types of BoCCA1 alleles were detected: BN106-type (freezing-tolerant; BoCCA1-1) and BN107-type (freezing-susceptible; BoCCA1-2). Numerous insertions/deletions (InDels), simple sequence repeats, and single nucleotide polymorphisms (SNPs) were found in the exons and introns of BoCCA1s from the ATG start codon at the second exon to the TGA stop codon at the eighth exon. Using InDels and SNPs, we designed PCR primer pairs to distinguish the freezing-tolerant lines, and validated these markers using 102 cabbage lines and cultivars. The inbred lines possessed either the BN106-type or BN107-type allele, but most cultivars had both alleles. Freezing-tolerant cabbage plants had BN106-type InDels and/or BN106-type SNPs regardless of the presence of BN107-type InDels and SNPs, and BN106-type SNPs were more widely detected in the freezing-tolerant cabbage plants than BN106-type InDels. The expression patterns of BoCCA1-1 and BoCCA1-2 were similar under normal versus temperature-stressed conditions (low and high temperatures), suggesting a functional difference at the post-transcriptional level. Cabbage breeders should use several markers derived from different genes and independently established inbred lines from different seed companies.
      PubDate: 2018-04-20
      DOI: 10.1007/s13580-018-0045-8
  • Postharvest quality maintenance and bioactive compounds enhancement in
           ‘Taaptimjaan’ wax apple during short-term storage by salicylic acid
    • Authors: Suriyan Supapvanich; Preyanuch Mitsang; Pannipa Youryon; Chairat Techavuthiporn; Panida Boonyaritthongchai; Racha Tepsorn
      Abstract: Physicochemical quality and bioactive compounds of ‘Taaptipjaan’ wax apple (Syzygium samarangenese) that were immersed in 0, 0.5 and 1.0 mM salicylic acid (SA) for 30 min and then stored at 12 ± 1 °C for 9 days were investigated. SA had no effect on the colour of fruit skin, total soluble solids, total acidity, total sugars, ascorbic acid (AsA) and anthocyanin contents but could help maintaining the firmness of the fruit. Antioxidant activity, total phenols, total flavonoids and antioxidant enzymes activities such as peroxidase and catalase were enhanced by SA. The SA immersion at the concentration of 0.5 mM effectively maintained firmness and visual appearance without causing any shrinkage on the fruit top and skin pitting and enhanced the bioactive compounds greater than 1.0 mM SA immersion. The evidences suggest that 0.5 mM SA immersion is an effective approach of maintaining quality and enhancing bioactive compounds including the activities of antioxidant enzymes in the fruit during short-term storage.
      PubDate: 2018-04-19
      DOI: 10.1007/s13580-018-0044-9
  • Effects of high temperature on in vitro tuberization and accumulation of
           stress-responsive proteins in potato
    • Authors: Danijel Pantelić; Ivana Č. Dragićević; Jelena Rudić; Jianming Fu; Ivana Momčilović
      Abstract: Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.
      PubDate: 2018-04-13
      DOI: 10.1007/s13580-018-0043-x
  • Control of relative humidity and root-zone water content for acclimation
           of in vitro-propagated M9 apple rootstock plantlets
    • Authors: Sang-Min Ko; Jin-Hui Lee; Myung-Min Oh
      Abstract: The present study aimed to evaluate the effects of controlling the relative humidity (RH) and water content of the root-zone on the survival rate and growth of in vitro-propagated virus-free M9 apple plantlets in closed-type plant production systems. In the first experiment, three RH regimes were applied to pre-acclimated (PA) and non-PA apple plantlets for 6 weeks after transplantation. In the second experiment, the apple plantlets were transplanted into several growth media, including a mixture of peat moss and perlite (PP), rock wool (RW), and urethane sponge (SP), and in a deep flow technique (DFT) system for controlled root zone water content under controlled RH. In the first experiment, pre-acclimation improved the survival rate by preventing the loss of leaf water potential and promoting antioxidant capacity during the acclimation period. However, no clear difference was found among the three RH regimes. The antioxidant capacity was increased at 2 weeks after transplantation, followed by root initiation. The leaf water potential, which decreased continuously until 3 weeks after transplanting, tended to remain constant after root initiation. These results suggested that pre-acclimation is necessary for the survival of in vitro-propagated apple plantlets, and that the underdeveloped roots of apple plantlets have restricted water absorption under controlled RH. In the second experiment, the survival rate of plantlets grown in PP at 6 weeks after transplantation was only 70% accompanied by an increase in antioxidant capacity, whereas the survival rates of plantlets grown in RW, SP, DFT, and DFT-PP (replanted to PP from DFT 4 weeks after transplantation) were 98, 96, 93.8, and 93.8%, respectively. Most of the growth parameters of the plantlets grown in DFT were the highest among the growth media at 6 weeks after transplantation. The results of the second experiment implied that the application of DFT for in vitro-propagated apple plantlets can reduce the problems caused by poor root architecture during acclimation.
      PubDate: 2018-04-13
      DOI: 10.1007/s13580-018-0038-7
  • Effect of cultivar and growing medium on the fruit quality attributes and
           antioxidant properties of tomato ( Solanum lycopersicum L.)
    • Authors: Shimeles Tilahun; Mu Hong Seo; Do Su Park; Cheon Soon Jeong
      Abstract: The objective of this research was to identify the growing medium that yielded the highest nutritional quality and longest marketable shelf life in tomato fruits. ‘TY Megaton’ and ‘Yureka’ cultivars were grown on soil and coir pith in the same climate-controlled glasshouse using a standard nutrient solution and the recommended cultivation practices. Fruits were harvested at the pink stage of ripening and stored at 12 °C in 85 ± 5% relative humidity for 20 days. The fruits of both cultivars grown on either growing medium were of acceptable quality for sale after 3 weeks of storage. The contents of the most important secondary metabolites of tomato responsible for providing their antioxidant activity (ascorbic acid, lycopene, and polyphenols) were not significantly affected by the choice of growing medium; however, significant differences were observed between the cultivars throughout the storage period. The results of this study demonstrated that the choice of cultivar is more important for fruit quality than the growing medium. The lycopene content and antioxidant activity of the fruits suggest that it is possible to achieve optimum nutrition from the pink-stage fruit of both cultivars after 12 days of storage, irrespective of the growing medium used.
      PubDate: 2018-03-14
      DOI: 10.1007/s13580-018-0026-y
  • Expression, glycosylation, and function of an anti-rabies virus monoclonal
           antibody in tobacco and Arabidopsis plants
    • Authors: Ilchan Song; Sol-Ah Park; Dalmuri Han; Hae Kyung Lee; Hyun Joo An; Kisung Ko
      Abstract: Plants have emerged as one of the most attractive systems for producing human therapeutic proteins against viral diseases. These include diagnostic reagents, vaccines, and antibodies. This process is known as molecular biofarming. The objective of this study was to develop and evaluate tobacco and Arabidopsis as plant platforms for producing human anti-rabies monoclonal antibody (mAb). Both tobacco and Arabidopsis transgenic plants were generated by Agrobacterium-mediated transformation. Purification of mAb SO57K from each plant was performed with ammonium sulfate-mediated precipitation and protein A affinity columns. SDS–PAGE analysis showed that the purity of mAb SO57K obtained from each transgenic plant was similar, whereas Arabidopsis showed approximately twofold greater protein expression than tobacco. The N-glycosylation was not significantly different between proteins from the two plant species, with both showing oligo-mannose glycan structures. The mAbs SO57 derived from both the model plants had similar neutralizing efficacy against target virus strain CVS-11. Taken together, tobacco and Arabidopsis are both promising platforms for producing a human anti-rabies mAb.
      PubDate: 2018-03-14
      DOI: 10.1007/s13580-018-0031-1
  • CO 2 enrichment increased leaf initiation and photosynthesis in
           Doritaenopsis Queen Beer ‘Mantefon’ orchids
    • Authors: Do Lee Yun; Hyun Jin Kim; Yoon Jin Kim
      Abstract: The plants have potential on carbon sink because plants absorb carbon dioxide (CO2) in ambient and use that for photosynthesis. CO2 enrichment, which is a commonly used technique in vegetable culture, may gain importance in ornamental production, potentially shortening the growth period. However, compared with forests and crop plants, the studies of CO2 enrichment on orchids are relatively limited. This study was undertaken to investigate the effects of elevated CO2 concentrations on leaf initiation and photosynthesis in the orchid Doritaenopsis Queen Beer ‘Mantefon’. Leaf growth and photosynthetic characteristics were measured in the plants grown under 450 (ambient control), 800, and 1600 µmol mol−1 CO2 during the latter 6 h of the dark period for 36 weeks. The number of leaves increased with the elevated CO2 concentration, and the time to leaf initiation decreased with elevated CO2 concentration. However, leaf span and biomass was lower in the plants grown under the higher CO2 concentrations compared to the plants grown under ambient CO2. Maximum net CO2 uptake, transpiration rate, and stomatal conductance were higher in the plants grown under 1600 µmol mol−1 CO2 than in the plants grown under 450 µmol mol−1 CO2. The 800 and 1600 µmol mol−1 CO2 concentrations accelerated leaf initiation and net CO2 uptake. We found that concentrations of CO2 in the 800 and 1600 µmol mol−1 CO2 range were controlled for growth of Doritaenopsis orchids.
      PubDate: 2018-03-14
      DOI: 10.1007/s13580-018-0025-z
  • Development of Bi gene-based SNP markers for genotyping for bitter-free
           cucumber lines
    • Authors: Jelli Venkatesh; Kihwan Song; Joung-Ho Lee; Jin-Kyung Kwon; Byoung-Cheorl Kang
      Abstract: High contents of cucurbitacin compounds result in a bitter taste in cucumbers. The Bi locus mapped to chromosome 6 is responsible for cucumber bitterness. The Bi gene encodes oxidosqualene cyclase, a key enzyme in the cucurbitacin biosynthetic pathway. Two alleles of the Bi gene responsible for the phenotypic variation in bitterness differ by a single point mutation in the coding region. An efficient genotyping system is necessary to allow breeders to screen for bitterness alleles in their breeding programs. In the present study, the single nucleotide polymorphism located within the Bi gene, which is directly linked to cucumber bitterness, was utilized for gene-based marker development. We developed reliable and cost-effective high-resolution melting (HRM)- and Kompetitive Allele-Specific PCR (KASP)-based molecular markers (BiHRM1 and Bi-KASP). These gene-based markers should enhance the accuracy and effectiveness of marker-assisted selection for bitter-free cucumber lines in cucumber breeding programs.
      PubDate: 2018-03-14
      DOI: 10.1007/s13580-018-0029-8
  • Calcium chloride and calcium gluconate peduncle infiltrations alleviate
           the internal browning of Queen pineapple in refrigerated storage
    • Authors: Pannipa Youryon; Suriyan Supapvanich; Pornprapa Kongtrakool; Chalermchai Wongs-Aree
      Abstract: This study determined the effects of calcium chloride (CaCl2) and calcium gluconate (CaGlu) in alleviating the incidence of internal browning (IB) in ‘Queen’ pineapple (Ananas comosus) cv. ‘Sawi’ during storage at 13 °C. Pineapples were vertically peduncle-infiltrated with 2% CaCl2 or 2% CaGlu for 48 h. IB and browning-related factors of tissue adjacent to the core were investigated after 7 and 14 days of cold storage followed by 2 days at room temperature. CaGlu treatment alleviated IB intensity and severity more effectively than CaCl2. CaCl2 and CaGlu retarded membrane peroxidation and the activity of browning enzymes such as phenylalanine ammonia-lyase and polyphenol oxidase and increased the total phenols in the fruit. However, CaGlu-treated fruit had lower levels of tissue electrolyte leakage and lipoxygenase activity and higher antioxidant capacity and antioxidant enzyme activity. This evidence suggests that CaGlu peduncle infiltration is the preferred choice for alleviating IB in ‘Queen’ pineapple during low temperature storage.
      PubDate: 2018-03-13
      DOI: 10.1007/s13580-018-0028-9
  • Growth and propagation rate of strawberry transplants produced in a plant
           factory with artificial lighting as affected by separation time from stock
    • Authors: Seon Woo Park; Yurina Kwack; Changhoo Chun
      Abstract: The suitable size of runner plants based on separation time from their stock plants was determined to maximize propagation rate of strawberry transplants using autotrophic transplant propagation method, a novel propagation method in a plant factory with artificial lighting for transplant production (T-PFAL). When runner tips with unfolded bracts were fixed on a growing medium to generate runner plants, the first true leaf, a runner, and roots of the runner plants appeared 6–10 days after fixing the runner tips (DAF), and their shoot and root dry weights significantly increased 6 and 10 DAF, respectively. The top/root ratio was the greatest 10 DAF. The net photosynthetic rate of runner plants 9, 11, and 13 DAF decreased after separation, while that at 15, 17, and 19 DAF did not. The runner plants separated from their stock plants 15, 20, and 25 DAF were successfully grown until 30 DAF; however, those 10 DAF was not. At 30 DAF, the root dry weight of the runner plants separated from the stock plants 15 DAF was smaller than that of the runner plants separated 20, 25, and 30 DAF, whereas the dry weights of leaves and runners were not significantly different. The use of small stock plants could reduce the required timescale from placing stock plants to produce new runner plants, but there was no significant difference in the timescales when the runner plants were separated 15 or 20 DAF due to the relatively insufficient growth of runner plants separated 15 DAF. These results indicate that runner plants separated 20 DAF, with two true leaves and a 5-mm crown diameter, would be suitable stock plants for the autotrophic transplant propagation method in a T-PFAL for strawberry propagation.
      PubDate: 2018-03-13
      DOI: 10.1007/s13580-018-0027-x
  • The effect of light quality on growth and endopolyploidy occurrence of in
           vitro-grown Phalaenopsis ‘Spring Dancer’
    • Authors: A-Reum Kwon; Myung-Min Oh; Kee-Yoeup Paek; So-Young Park
      Abstract: In the present study, the effect of light quality on endoreduplication and growth in Phalaenopsis ‘Spring Dancer’ plantlets was studied. The response of protocorm-like body (PLB)-derived plantlets subjected to monochromatic red (R60), blue (B60), and various combinations of both lights was investigated. Flow cytometry was used to investigate the effect of light on endocycle and growth, cell division, and endopolyploidy levels. In addition, the activities of stress-related enzymes such as catalase (CAT) and peroxidase (POD) were analyzed from leaves and roots. After 8 weeks, the leaf area of plants grown under monochromatic R60 and B60 light was found to be higher than that of plants grown under other wavelengths of light, except the control plants (fluorescent light). These results revealed monochrome blue (B60) light increased the ratio of endoreduplicated cells (4C–8C). CAT activity was highest in leaves grown under R60; however, the oxidized phenol concentration in the culture medium was lowest under R60 while it was the highest under B60 and fluorescent light (F). This indicates that plantlets were less stressed under R60 than B60 or F. The results of this study reveal that stress induced by monochromatic light stimulates endopolyploidy in leaves, which may subsequently increase Phalaenopsis leaf size.
      PubDate: 2018-03-13
      DOI: 10.1007/s13580-018-0018-y
  • Effects of backcrossing on quality of strawberry fruit
    • Authors: Sun Yi Lee; Seung Yu Kim; Dae Young Kim; Ho Jeong Jeong; In Seok Um; Il Rae Rho
      Abstract: American/European strawberry cultivars are morphologically different from Asian cultivars with American cultivars generally having higher yields, fruit weight, and fruit hardness and Asian cultivars tending to have higher sugar content. In this study, we performed backcrossing to improve the fruit qualities of Asian varieties of strawberries using American cultivars as the donor parent. The F1 progeny derived from crosses between Asian and American cultivars tended to have lower sugar content and higher fruit weight and yield than Asian cultivars, but fruit hardness did not differ between the Asian cultivars and the progenies. The percent germination and survival ratio were not significantly different between the BC1F1 and BC2F1 generations, whereas the percent germination decreased rapidly with advancing backcross generations. The BC1F1 generation had slightly higher sugar content and the sugar content of the BC2F1 generation increased significantly over the BC1F1 generation, but fruit weight and yield decreased. However, there was variability among individual progeny from the same cross. With advancing generations of backcrossing (BC3F1, BC4F1, BC5F1), sugar content tended to improve while fruit weight and yield tended to decrease, and hardness tended not to change. Therefore, to develop new cultivars with the combined qualities of Asian and American cultivars, we concluded that generations beyond the BC2F1 are not necessary, and that the selection efficiency of superior individual plants can be improved if the number of seedlings is increased in generations before the BC1F1 or BC2F1.
      PubDate: 2018-02-19
      DOI: 10.1007/s13580-018-0023-1
  • Morphological and genetic diversity of Moringa oleifera and Moringa
           peregrina genotypes
    • Authors: Anber Mahmoud Ahmed Hassanein; Abdulrahman Abdulah Al-Soqeer
      Abstract: This study aimed to assess the diversity of Moringa oleifera and Moringa peregrina in Saudi Arabia. Seven genotypes per species were characterized morphologically using 14 morphological characteristics and genetically using 10 ISSR primers. The studied genotypes were classified according to each characterization, and the correlation between morphological and genetic diversity was investigated. M. oleifera genotypes were distinguished by long, thick stems with more crown spread, and larger pinnate leaf area compared to those of M. peregrina. Plant height and pinnate dimensions were the most pertinent indicators for the discrimination among genotypes where they were correlated to all morphological characteristics and gave representative classification. Diversity was found between the two species and among the genotypes of each species. The ISSR molecular markers were effective in the characterization of genetic diversity of Moringa where the average of polymorphism across the 14 genotypes was sufficient (90.8%). Dinucleotide repeat (AC)n primers (UBC825, UBC826 and UBC827) and a trinucleotide primer (UBC864) were the best primers, regenerating the maximum number of polymorphic bands per primer (8–10) and the highest polymorphism level among genotypes (91–100%). Principal coordinate analysis showed similar classification for morphological and molecular data where the two species were separated in two main clusters with three sub-clusters per species. The association analysis showed good correlation, up to a 0.84 determination coefficient, between genetic diversity and morphological variability. The primers UBC826 and UBC827 were the most informative markers, revealing correlations with 12 morphological characteristics. The results of the present study provide valuable morphological and molecular characterizations of the two most important Moringa species. Efficient morphological classification based on three characteristics could facilitate the evaluation of diversity in Moringa. Genetic diversity could be simply assessed using the two best ISSR primers (UBC826 and UBC827). The diversity found among genotypes could be of great importance for the selection of clones with desirable characteristics for further improvements of Moringa.
      PubDate: 2018-02-19
      DOI: 10.1007/s13580-018-0024-0
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