for Journals by Title or ISSN
for Articles by Keywords
help
  Subjects -> BIOLOGY (Total: 3149 journals)
    - BIOCHEMISTRY (247 journals)
    - BIOENGINEERING (116 journals)
    - BIOLOGY (1495 journals)
    - BIOPHYSICS (47 journals)
    - BIOTECHNOLOGY (237 journals)
    - BOTANY (231 journals)
    - CYTOLOGY AND HISTOLOGY (29 journals)
    - ENTOMOLOGY (68 journals)
    - GENETICS (166 journals)
    - MICROBIOLOGY (266 journals)
    - MICROSCOPY (11 journals)
    - ORNITHOLOGY (26 journals)
    - PHYSIOLOGY (73 journals)
    - ZOOLOGY (137 journals)

BIOTECHNOLOGY (237 journals)                  1 2 | Last

Showing 1 - 200 of 237 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 14)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 8)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 10)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 64)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 31)
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 8)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 43)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 63)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 8)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 5)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 155)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 5)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 13)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 16)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 16)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 2)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 56)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 12)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 13)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Biotechnology and Strategic Health Research     Open Access  
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 24)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 11)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 2)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 535)

        1 2 | Last

Journal Cover
Biocatalysis and Agricultural Biotechnology
Journal Prestige (SJR): 0.479
Citation Impact (citeScore): 2
Number of Followers: 4  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Online) 1878-8181
Published by Elsevier Homepage  [3163 journals]
  • Anticoagulation, fibrinolytic and the cytotoxic activities of sulfated
           hemicellulose extracted from rice straw and husk
    • Authors: Tamer I.M. Ragab; Hassan Amer; Abdel Tawab Mossa; Mahmoud Emam; A.A. Hasaballah; Wafaa A. Helmy
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Tamer I.M. Ragab, Hassan Amer, Abdel Tawab Mossa, Mahmoud Emam, A.A. Hasaballah, Wafaa A. Helmy
      Rice straw and husk are one of the major agricultural wastes in Egypt, which cause problems for farmers. Hemicellulose is considered one of the major components of straw and husk lignocellulosic biomass. The new methods and techniques for hemicellulose extraction from renewable with low cost and their modification to produce a promising value-added hemicellulose derivative are important issues. Therefore, the present study aimed at extracting hemicellulose from Egyptian agriculture wastes rice straw and husk by 4% sodium hydroxide at 90 °C. The extracted hemicellulose was purified by 5% hydrogen peroxide and separated into soluble and insoluble hemicellulose in distilled water. The sulfation process of hemicellulose was done in presence of two different catalysts (N, N-Dicyclohexylcarbodiimide (DCC) and 4-Dimethylaminopyridine (DMAP)). Results showed that the sulfated soluble hemicelluloses have the highest degree of sulfation (DS) with low total carbohydrate content. The prepared sulfated hemicellulose using DCC or DMAP showed promising biological activities such as anticoagulation activity at 31.25 µg/mL and fibrinolytic activity lysis more than 80% at 2000 µg/mL compared standard (Hemoclar). These sulfated hemicellulose compounds were practically non-toxic on the VERO cells with LD50 ≥ 5000 mg /Kg.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.010
      Issue No: Vol. 15 (2018)
       
  • Phytochemical composition, in vitro antioxidant, antibacterial potential
           and GC-MS analysis of red seaweeds (Gracilaria corticata and Gracilaria
           edulis) from Palk Bay, India
    • Authors: Abimannan Arulkumar; Thomas Rosemary; Sadayan Paramasivam; Ramaswamy Babu Rajendran
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Abimannan Arulkumar, Thomas Rosemary, Sadayan Paramasivam, Ramaswamy Babu Rajendran
      Increasing of resistance pathogenic microorganisms to majority of antibiotics, there is an urgent need for exploring plant based drugs and bioactive compounds with least side effects. The study was aimed to determine the level of phytochemicals, antioxidant, antibacterial properties of the edible red seaweeds, Gracilaria corticata and G. edulis. The extraction with methanol yielded 7.10 ± 0.16 and 6.39 ± 0.16% extracts from G. corticata and G. edulis respectively. The G. corticata possess higher total phenol content (4.00 ± 0.35 mg GAE/g) compare to G. edulis (3.4 ± 0.21 mg GAE/g). G. corticata and G. edulis extracts significantly varied in total flavonoid content i.e 3.33 ± 0.12 and 2.6 ± 0.08 mg CE/g DW respectively. In this investigation, G. edulis presented the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (23.95%) when compare to G. corticata (20.32%). G. edulis showed significantly higher 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (40.24%) compare to G. corticata (32.65%). In addition, G. corticata exhibited higher nitric oxide (NO*) radical scavenging activity (36.78%) than G. edulis 35.25%. Antimicrobial properties of 70% methanol and DMSO extracts were found effective against Bacillus subtilis. GC-MS analysis revealed the presence of phytochemical compounds including sulfurous acid, 2-ethylhexyl isohexyl ester, pentatriacontane, eugenol and phthalic acid played a vital role in antioxidant and antibacterial activities.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.008
      Issue No: Vol. 15 (2018)
       
  • Experimental investigation of the suitability of 1-butanol blended with
           biodiesel as an alternative biofuel in diesel engines
    • Authors: Jeya Jeevahan; R.B. Durairaj Sriramanjaneyulu; G. Mageshwaran
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Jeya Jeevahan, G. Sriramanjaneyulu, R.B. Durairaj, G. Mageshwaran
      Vegetable oils, biodiesels, bio-alcohols and bio-gas are some of the popular biofuels evaluated for their suitability in compression-ignition (CI) engines. Among these, bio-alcohols can be produced from any kinds of biomass through fermentation and biosynthesis and they do not require extra land for cultivation. Therefore, bio-alcohols can be considered as the next generation alternative fuels for automobiles. Investigations have been already initiated to determine the effects of bioalcohols, such as methanol and ethanol, in automobiles as fuel. However, they pose some problems like miscibility, phase separation, low cetane number and low calorific value. On the other side, the effects of higher alcohols, such as butanol, pentanol, octanol, are investigated very rarely. In this work, the effects of higher alcohol addition on the engine performance and emissions characteristics are investigated on a single cylinder diesel engine. Conventional diesel and biodiesel are taken as the reference fuels. 1-Butanol of blends (10%, 20%, 30%, 40%, and 50%) was mixed with the remaining biodiesel as the testing fuel blends. Experiments were conducted on a single cylinder compression ignition diesel engine for four load conditions (5 kg, 10 kg, 15 kg and 20 kg) at a constant speed of 1500 rpm. Brake thermal efficiency and emissions of CO, NOx and HC were recorded and discussed. From the experimental results, it is evident that the addition of butanol with biodiesel seems to an alternative fuel that can replace conventional diesel fuel in terms of both engine emissions and performance.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.013
      Issue No: Vol. 15 (2018)
       
  • Solid-state cultivation of recombinant Aspergillus nidulans to co-produce
           xylanase, arabinofuranosidase, and xylooligosaccharides from soybean fibre
           
    • Authors: Gabriela Feix Pereira; Daniela de Bastiani; Sabrina Gabardo; Fabio Squina; Marco Antônio Záchia Ayub
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Gabriela Feix Pereira, Daniela de Bastiani, Sabrina Gabardo, Fabio Squina, Marco Antônio Záchia Ayub
      Solid-state cultivations of genetically modified strains of Aspergillus nidulans A773, using soybean fibre as substrate, were carried out to produce xylanase and arabinofuranosidase, and these enzymes were subsequently used to obtain xylooligosaccharides using the same agro-residue. First, the best fungi cultivation conditions (moisture content, pH, temperature and addition of maltose) were optimized one-by-one for obtain the crude enzyme extracts. Subsequently, the application of xylanase on soybean fibre to obtain xylooligosaccharides was optimized by central composite design, defining best enzyme concentration and reaction temperature. The best condition obtained (50 °C and 117 U g−1 of soybean fibre) was used to evaluate the co-production of xylooligosaccharides by the addition of different concentrations of arabinofuranosidase. The highest yield of xylooligosaccharides obtained was 28% (mass fraction of xylan), showing final concentrations (in mg g−1 arabino-xylan) of 138.36 xylobiose (X2), 96.96 xylotriose (X3), and 53.04 xylotetraose (X4), in 9 h enzymatic reactions. The conversion of arabino-xylans into different xylooligosaccharides suggests the potential to use recombinant A. nidulans A773 enzymes to obtain prebiotics using a sugar-rich, low-cost soybean residue.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.012
      Issue No: Vol. 15 (2018)
       
  • A cold tolerant lipase develops enhanced activity, thermal tolerance and
           solvent stability in the presence of calcium nanoparticles: An alternative
           approach to genetic modulation
    • Authors: Aniket Das; Krishanu Chakrabarti
      Pages: 1 - 10
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Aniket Das, Krishanu Chakrabarti
      An extracellular cold active lipase producing bacterium was isolated from soil. It was identified as Pseudomonas aeruginosa KC1 strain (GenBank accession number KT334371). There are no previous reports on purification of cold active lipase protein from Pseudomonas aeruginosa. The lipase of molecular weight 54 kDa was purified to 33 fold with 8% recovery. The enzyme was active within the range 10–40 °C with maximum activity at 15 °C; pH 7.0–8.5 with 4-nitrophenyl butyrate substrate. Substrate utilization by lipase showed better affinity for short chain fatty acid esters. The enzyme activity was enhanced by Ca2+, Ba2+, Fe2+ and Mg2+; inhibited by Hg2 +, Ni2+, Zn2+ and Co2+. Nano-calcium enabled KC1 lipase (NP-lip) showed enhanced activity for both short and long chain fatty acid esters (NP: 8.8 µg/mL) compared to CaCl2 (1 mM). The activity of the NP-lip system increased 72% at 15 °C and 7 fold at 55 °C while retaining activity for 4 h. Lowering of K m (55% at 15 °C; 45% at 55 °C) and increased V max (7 fold at 15 °C; 3.5 fold at 55 °C) was observed for NP-lip system. Heat deactivation kinetics for NP-lip showed drastic improvement in half-life at higher temperatures and entropy-enthalpy compensation. Furthermore the NP-lip system was stable in organic solvents and was effective in the esterification of butyl butyrate and trans-esterification of sunflower oil in n-hexane. This remarkable simultaneous enhancement of activity, temperature and organic solvent tolerance of the NP-lip has potential for industrial usage.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.002
      Issue No: Vol. 15 (2018)
       
  • Promising biocontrol agents isolated from medicinal plants rhizosphere
           against root-rot fungi
    • Authors: Moustafa M. Zohair; Ahmed A. El-Beih; Mahmoud W. Sadik; Eman R. Hamed; Mohamed Z. Sedik
      Pages: 11 - 18
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Moustafa M. Zohair, Ahmed A. El-Beih, Mahmoud W. Sadik, Eman R. Hamed, Mohamed Z. Sedik
      Among of the collection of 104 fungal isolates from the rhizosphere of the medicinal plants basil (Ocimum basilicum), peppermint (Mentha piperita) and (Aloe vera), 59 of them were assessed for in vitro antagonistic activity against phytopathogenic fungi; Fusarium solani, Rhizocotina solani, Sclerotium rolfsii and Verticillium dahliae. The most active antagonistic isolates were identified using molecular tools based on 18S rDNA. The sequence data of Aspergillus pseudocaelatus and Trichoderma gamsii have been submitted to GenBank given the accession no. MG772677 & KX685665, respectively. The antagonistic mechanisms were evaluated using confrontation method and scanning electron microscopy. The volatile organic compounds (VOCs) were analyzed using GC/MS. Biological investigation on the ethyl acetate extract of A. pseudocaelatus MG772677 and T. gamsii KX685665 isolates was evaluated. The greenhouse application with the selected isolates on basil seedlings was performed. In vitro antagonistic results indicated that the highest percentage of inhibition was observed with A. pseudocaelatus, 77.90% and T. gamsii, 77.98%. The average emergence rate in the treatments with T. gamsii and A. pseudocaelatus reached up to 100%, much more than that in the control (average 40%). The results indicated that A. pseudocaelatus MG772677 and T. gamsii KX685665, displayed antagonistic activities against the pathogenic fungi and presented appreciable biocontrol efficacy. Also the two isolates could enhance the plant growth and improve the seedlings’ emergence.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.04.015
      Issue No: Vol. 15 (2018)
       
  • Ethanol tolerant endoglucanase from Aspergillus niger isolated from wine
           fermentation cellar
    • Authors: Dong sheng Xue; Xuhao Zeng; Dongqiang Lin; Shanjing Yao
      Pages: 19 - 24
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Dong sheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao
      The endoglucanase from Aspergillus niger isolated from a wine fermentation cellar was ethanol tolerant. Optimum pH and temperature of the endoglucanase activity was 5.0 and 60 °C. The endoglcuanase activity in 8% ethanol solution was higher than that in ethanol-free solution. Melting point temperature of the endoglucanase in 8% ethanol solution was 1.9 °C higher (40.0 °C) than that in ethanol-free solution (37.1 °C). At 45–60 °C, Gbbs free energe, ΔG, in 8% ethanol solution was higher than that in ethanol-free solution. The α-helix content increased in 8% ethanol solution. The increasing of α-helix content led to higher activity and better thermostability in high concentration ethanol solution. The acidic, thermostable and ethanol tolerant endoglucanase was valuable for bioethanol production by simultaneous saccharification and fermentation process.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.04.016
      Issue No: Vol. 15 (2018)
       
  • Biosorption of low concentration levels of Lead (II) and Cadmium (II) from
           aqueous solution by Saccharomyces cerevisiae: Response surface methodology
           
    • Authors: Mohammad Rasoul Hadiani; Kianoush Khosravi Darani; Nahid Rahimifard; Habibollah Younesi
      Pages: 25 - 34
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Mohammad Rasoul Hadiani, Kianoush Khosravi Darani, Nahid Rahimifard, Habibollah Younesi
      The present study refers to application of Saccharomyces cerevisiae for bioremoval of very low amounts of heavy metals from aqueous media. The impact of process variables on biosorption of lead (II) and cadmium (II) by S. cerevisiae was first evaluated with Taguchi screening design. After determining of significant variables, optimization of biosorption process was performed by 3 independent parameters (pH, biomass, initial ion concentration) using a central composite design. So, twenty test runs were done and the experimental data fitted to the second-order polynomial models. The analysis of variance of the quadratic models showed that the models are highly significant. The adjusted conditions in the best set was 5, 52.5 µg/l and 32.5 × 107 CFU for pH, heavy metal concentration and biomass, respectively. Behavior of biosorption system in a batch process was analyzed by 3D plots which represented dual simultaneous interaction effects of variables on metal biosorption yield. The model explained that heavy metal biosorption in aqueous solution is affected by all the three factors studied. An optimum lead (II) and cadmium (II) biosorption yield of 70.3% and 76.2% were found at initial ion concentration of 65.0 and 62.6 µg/l, and S. cerevisiae of 15.0 and 15.2 × 107 CFU, respectively. The results suggest that S. cerevisiae, as a natural, low-cost and abundant sorbent, has the potential to be used in biosorption of very low concentrations of lead and cadmium, which is useful to reduce the contaminations of drinking water and foodstuff with green technologies.
      Graphical abstract image

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.001
      Issue No: Vol. 15 (2018)
       
  • Influence of diazotrophic bacteria on nodulation, nitrogen fixation,
           growth promotion and yield traits in five cultivars of chickpea
    • Authors: Subramaniam Gopalakrishnan; Vadlamudi Srinivas; Anilkumar Vemula; Srinivasan Samineni; Abhishek Rathore
      Pages: 35 - 42
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Subramaniam Gopalakrishnan, Vadlamudi Srinivas, Anilkumar Vemula, Srinivasan Samineni, Abhishek Rathore
      Three bacteria, IC-59, IC-76A and IC-2002, isolated from the nodules of chickpea, were characterized for nodulation, nitrogen fixation, plant growth-promoting (PGP) and yield traits in five cultivars of chickpea such as BG256, RSG888, Subhra, K850 and ICCV2. All the bacteria produced cellulase, protease, β-1,3-glucanase, indole acetic acid, siderophore, hydro cyanic acid and 1-aminocyclopropane-1-carboxylate deaminase while none produced lipase and chitinase. The 16 S rDNA gene sequences of IC-59, IC-76A and IC-2002 were found to match closely with Rhizobium pusense, Paraburkholderia kururiensis and Stenotrophomonas maltophilia, respectively. The three bacteria nodulated all the cultivars of chickpea well, amplified nifH gene and fixed nitrogen. Under greenhouse conditions at 30 and 45 days after sowing, treatment of five cultivars of chickpea with bacterial cultures IC-59, IC-76A and IC-2002, enhanced the nodule number (up to 45%, 38% and 43%), nodule weight (up to 31%, 15% and 39%), shoot weight (11%, 16% and 14%) and root weight (37%, 48% and 62%), respectively, over the un-inoculated control. At crop maturity, IC-59, IC-76A and IC-2002 were found to enhance the shoot weight (16%, 40% and 26%), pod number (37%, 69% and 81%), pod weight (17%, 45% and 49%), seed number (21%, 31% and 39%) and seed weight (14%, 56% and 65%), respectively, over the un-inoculated control. Among the five cultivars, Subhra was found to enhance most of the PGP traits when treated with the three diazotrophic bacteria. It is concluded that the three diazotrophic bacteria could be potentially exploited for improving nodulation, nitrogen fixation, PGP and yields of chickpea.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.006
      Issue No: Vol. 15 (2018)
       
  • Collagen and collagenolytic proteases: A review
    • Authors: Prashant K. Bhagwat; Padma B. Dandge
      Pages: 43 - 55
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Prashant K. Bhagwat, Padma B. Dandge
      Despite of having enormous applications, the use of collagen is predominantly limited because of its high cost. Most of the mammalian sources used for its production have major drawbacks. However, compared to mammalian sources, fish waste can be utilized as cost-effective alternative to produce collagen. Around 75% part of fish is discarded as a waste which contains high concentration of collagen. Fish collagen has multiple advantages over mammalian collagen and hence can be a promising alternative for it. Proteases with collagenolytic activities are also of immense importance because of their industrial as well as biological applications. Microbial collagenolytic proteases are gaining huge attention in these days because of their lower requirements and higher productivity. They perform important role in global recycling of collagenous waste. This review gives recent information on collagen and collagenolytic proteases. Here, utilization of seafood by-products is discussed to recover the collagen and its recent applications are summarized. In addition to this, current review also highlights the recent status of collagenases in which present strategies and new technology used for the isolation, screening, production optimization, purification, characterization and applications of microbial collagenases are discussed.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.005
      Issue No: Vol. 15 (2018)
       
  • Biosynthesis, characterization and antibacterial activity of copper oxide
           nanoparticles (CuO NPs) from actinomycetes
    • Authors: Mohammed Ishaque Nabila; Krishnan Kannabiran
      Pages: 56 - 62
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): Mohammed Ishaque Nabila, Krishnan Kannabiran
      The aim of the present study was actinomycetes mediated biosynthesis of copper oxide nanoparticles (CuO NPs) and evaluation of its antibacterial activity against selected human and fish pathogens. The biosynthesized CuO NPs were characterized by UV–Visible (UV-Vis) spectroscopy. The presence of capping agents over the metal nanoparticles was confirmed by Fourier-Transform Infrared Spectroscopy (FT-IR). The crystalline nature of the CuO NPs was illustrated by X- Ray diffractometer (XRD). The average size of the biosynthesized CuO NPs from XRD and Transmission Electron Microscopy (TEM) was 61.7 nm. The XRD and Energy Dispersive X-Ray spectroscopy (EDX) suggests the purity of the biosynthesized CuO NPs. The morphology and size was viewed under Scanning Electron Microscopy (SEM). The Dynamic Light Scattering (DLS) results provided the zeta potential of − 31.1 mV which further confirmed the stability of the CuO NPs. The biosynthesized CuO NPs showed higher antibacterial activity (zone of inhibition) against various human and fish bacterial pathogens (Staphylococcus aureus, Bacillus cereus, Proteus mirabilis, Edwardsiella tarda, Aeromonas caviae, Aeromonas hydrophila and Vibrio anguillarum). The antibacterial activity of CuO NPs was significantly higher than the activity exhibited by cell free supernatant of actinomycetes. Among the pathogens tested B. cereus was more susceptible (25.3 mm) to biosynthesized CuO NPs. The antibacterial activity exhibited by the actinomycetes mediated biosynthesized CuO NPs suggests that it can combat both human as well as fish bacterial pathogens. To the best of our knowledge, this is the first report on actinomycetes mediated biosynthesis of CuO NPs.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.011
      Issue No: Vol. 15 (2018)
       
  • Fabrication, characterization and osseointegration of bonegraft
           incorporated with leaf extracts of Ormocarpum Sennoides and biocompatible
           polymers
    • Authors: S. Srividya; G. Sridevi; B. Santhosh Kumar; T.P. Sastry
      Pages: 92 - 102
      Abstract: Publication date: July 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 15
      Author(s): S. Srividya, G. Sridevi, B. Santhosh Kumar, T.P. Sastry
      Allogenous grafts with excellent biocompatibility and immuno-compatibility play a major role in most of the biomedical applications. In this regard, the present study focuses on fabricating a novel bone graft material containing biopolymers and phytochemicals which can replace the use of autogenous graft with high biocompatibility and osteogenecity. Based on this, a bone graft material was synthesized using biphasic calcium phosphate (BCP), casein (CA), hen egg yolk (EY) and leaf extracts of Ormocarpum sennoides (Os). Two types of bone grafts namely group I (BCP-EY) and II (BCP-CA-Os-EY) were prepared and processed. The processed grafts were subjected to various characterizations like Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), thermo gravimetric analysis (TGA), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, and mechanical strength to show its chemical composition, stability and porosity. Further, the osteogenecity of the grafts were analyzed by performing invivo studies using wistar male albino rats. Samples were subjected to biochemical, radiological and histopathological analyses. Among the two grafts, graft II containing Ormocarpum sennoides extract showed excellent osteogenicity both invitro and invivo and hence it can be utilized in various biomedical applications like orthopedics, dental fillings, bone tissue engineering and in the treatment of rheumatoid arthritis.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.004
      Issue No: Vol. 15 (2018)
       
  • Effects of macro/micronutrients on green and brown microalgal cell growth
           and fatty acids in photobioreactor and open-tank systems
    • Authors: Syed Muhammad Usman Shah; Mohd Azmuddin Abdullah
      Pages: 10 - 17
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Syed Muhammad Usman Shah, Mohd Azmuddin Abdullah
      This study described the optimization of growth parameters that affected locally-isolated green Nannochloropsis oculata and Tetraselmis suecica and brown Isochrysis galbana and Pavlova lutheri microalgae using Response Surface Methodology (RSM). Under deficiency conditions of 10–65 g L−1 KNO3, 3–7.5 g L−1 Na2HPO4 and 2.5 g L−1 FeCl3, the highest lipid accumulation of 37.3%, 23.6%, 28.3% and 37.2%, with slightly reduced cell growth of 0.64, 0.49, 0.54 and 0.38 g L−1 were achieved for N. oculata, T. suecica, I. galbana and P. lutheri, respectively. The macronutrients significantly influenced the biomass and lipid content positively. However, the interaction of phosphate-phosphate for N. oculata, and nitrate-nitrate for I. galbana may affect cell growth negatively. The highest biomass of 0.62–0.96 g L−1 and lipid content of 31.6–42.2% in 5 L PBR and the highest biomass of 0.45–0.72 g L−1 and lipid content of 24.4–38.5 in 300 L open tank were achieved for all the four species. The total saturated fatty acids (44.3–63.8% and 30.4–55.03%); monounsaturated fatty acids (6.1–37.0% and 4.2–13.1%); and polyunsaturated fatty acids (8.3–22.3% and 1.02–15.2%) were obtained, respectively, with pentadecanoic (C15:0), palmitic (C16:0), palmitoleic (C16:1), heptadecanoic (C17:0), oleic (C18:1), eicosanoic (C20:0), eicosapentaenoic (C20:5) and docosahexaenoic (C22:6) as predominant fatty acids.

      PubDate: 2018-02-07T16:27:46Z
      DOI: 10.1016/j.bcab.2018.01.011
      Issue No: Vol. 14 (2018)
       
  • An approach to enhance nutritive quality of groundnut (Arachis hypogaea
           L.) seed oil through endo mycorrhizal fertigation
    • Authors: Prerna B. Pawar; Jayshri P. Khadilkar; Mohan V. Kulkarni; Jose S. Melo
      Pages: 18 - 22
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Prerna B. Pawar, Jayshri P. Khadilkar, Mohan V. Kulkarni, Jose S. Melo
      Groundnut is the sixth most important oilseed crop in the world and India is the second largest groundnut producing country. There is a need of increasing the production of groundnut and stabilizing its yield by using proper agricultural practices. Application of arbuscular mycorrhizal fungi (AMF) has been considered as an important strategy for sustainable agricultural practices. Approach of the present study was to evaluate effectiveness of ten different indigenous mycorrhizal species viz. Glomus mosseae, Glomus clarum, Glomus fasciculatum, Glomus intraradices, Glomus ambisporum, Gigaspora gigantea, Acaulospora denticulata, Glomus globiferum, Gigaspora albida and Glomus pansiholus on oil content, acid value, fatty acid profile and elemental status of groundnut oil. All the mycorrhizal treatments showed significant results as compared to the control (non mycorrhizal plants), but Glomus mosseae was found to be the most superior of all the ten mycorrhizal species. Oil percentage, extracted from Glomus mosseae (41.66%) treated groundnut oil was higher as compared to control (28.50%). Oleic acid, linoleic acid, palmitic acid content from groundnut oil of mycorrhiza treated plants varied in range of 36–45%, 16–22%, 13–18% respectively. Oil from Glomus mosseae treated groundnut plant showed increase in zinc, calcium, magnesium, manganese content than other mycorrhiza treated groundnut seed oil and control. Oil extracted from mycorrhiza treated groundnut oil showed decrease in acid value (AV) than control, which indicates higher stability of oil and longer shelf life. Therefore, it is very important to select the correct and specific species of AMF for improving the oil profile of Groundnut (Arachis hypogaea L.)

      PubDate: 2018-02-07T16:27:46Z
      DOI: 10.1016/j.bcab.2018.01.012
      Issue No: Vol. 14 (2018)
       
  • Biosurfactants: Production and potential applications in microbial
           enhanced oil recovery (MEOR)
    • Authors: Geetha S.J.; Ibrahim M. Banat; Sanket J. Joshi
      Pages: 23 - 32
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Geetha S.J., Ibrahim M. Banat, Sanket J. Joshi
      Microbial enhanced oil recovery (MEOR) is a type of enhanced oil recovery (EOR) technology, generally employed as a tertiary stage where oil recovery using primary and secondary traditional methods is not feasible anymore. Amongst several potential biological agents useful for MEOR, biosurfactants (biologically produced amphiphilic surfactants) play key roles. They are mostly equivalent to or better than their chemical counterparts in several aspects including; better environmental compatibility, production from renewable waste substrates, maintaining activity at harsh environmental conditions, lower or no environmental toxicity. Biosurfactants are still not cost-competitive when compared to chemical surfactants. Different strategies like the use of cheaper raw materials, optimization of media components, fermentation processes and downstream processes, use of hyperproducers are currently explored to improve biosurfactant production economics. Biosurfactant mediated MEOR (BS-MEOR) could be applied by either in-situ or ex-situ techniques. In-situ BS-MEOR could be applied by injecting biosurfactant producing microorganisms in the oil well with or without additional nutrients. Generally followed by shut-in phase and subsequently monitoring microbial activities, metabolites production and oil recovery from the producer wells. Whereas for ex-situ BS-MEOR applications, the biosurfactant is produced outside the oil well and injected directly for enhancing oil recovery. This review highlights the biosurfactant production and economics, general protocols for applications from lab-to-field scale, different successful trials along with pros and cons of both in-situ and ex-situ BS-MEOR applications.

      PubDate: 2018-02-07T16:27:46Z
      DOI: 10.1016/j.bcab.2018.01.010
      Issue No: Vol. 14 (2018)
       
  • Screening of a biological control bacterium to fight avocado diseases:
           from agroecosystem to bioreactor
    • Authors: Sinar David Granada; Sara Ramírez-Restrepo; Lorena López-Luján; Carlos Alberto Peláez-Jaramillo; Juan Carlos Bedoya-Pérez
      Pages: 109 - 115
      Abstract: Publication date: Available online 19 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Sinar David Granada, Sara Ramírez-Restrepo, Lorena López-Luján, Carlos Alberto Peláez-Jaramillo, Juan Carlos Bedoya-Pérez
      Avocado diseases remain to be a major constraint for the development of this agribusiness, which is largely led by Latin American countries. In this study, a collection of 667 native avocado bacterial isolates was established and screened for antagonistic activity and bioactive secondary metabolite production against two important avocado pathogens (Phytophthora cinnamomi and Colletotrichum gloeosporioides). The isolate ARP5.1 demonstrated the highest potential for in vitro control of the phytopathogens and was identified as Serratia sp. To enhance bioactive metabolite production by ARP5.1, different combinations of agitation speeds and aeration rates were evaluated on a stirred tank bioreactor. The activity of the crude extracts was tested and cell growth kinetics, oxygen and glucose consumption, and production of secondary metabolites were determined at the fermentation conditions of higher inhibitory activity. Results evidenced oxygen as critical factor for the biosynthesis of metabolites of interest. In addition, shear stress and glucose were limiting factors in metabolite production. Prodigiosin, serratamolide and haterumalide NC were identified in the extracts by Liquid Chromatography coupled to High-Resolution Mass Spectrometry. However, the presence of haterumalide NC might explain the high activity of the crude extract against P. cinnamomi. We concluded that this bacterium might be a promising candidate for the control of avocado pathogens in field and a good source of bioactive metabolites.
      Graphical abstract image

      PubDate: 2018-02-26T18:34:24Z
      DOI: 10.1016/j.bcab.2018.02.005
      Issue No: Vol. 14 (2018)
       
  • Deposition of manure nutrients in a novel mycoalgae biofilm for Nutrient
           management
    • Authors: Aravindan Rajendran; Tyler Fox; Cristiano Rodrigues Reis; Bruce Wilson; Bo Hu
      Pages: 120 - 128
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Aravindan Rajendran, Tyler Fox, Cristiano Rodrigues Reis, Bruce Wilson, Bo Hu
      With the increase in intensive crop and livestock production, excess application of P as fertilizer and manure contributes to the build-up of soil P levels causing eutrophication. A novel biofilm-based technology was developed to recover and reposition the nutrients in manure, producing biofilm fertilizer and the treated water with better nutrient composition. Anaerobically digested and pretreated manure was used as a medium to grow the surface-attached composite biofilm, which constitutes the selected polyphosphate accumulating fungi and nitrogen accumulating fresh water microalgae for efficient recovery of nutrients on a matrix for better biomass harvesting. Under the tested conditions in lab-scale with the pretreated digested manure, the removal efficiency of the nutrients by attached mycoalgae biofilm was 76.74% P and 76.40% N with COD removal of 65.75%. To increase the nutrient content of the biomass and for enhancing the cell growth the wastewater generated in corn ethanol process (thin stillage) was added as an external nutrient at different ratios in the digested manure. The cell growth, nutrient removal efficiency, lipid content of the biomass, COD removal and reducing sugar content at different medium conditions were evaluated. The nutrient-rich solid biofilm can be harvested by scraping off the biofilm from the matrix and the nutrient lean liquid can be discharged or further used for agriculture. The microbial biofilm assimilates the organic and inorganic components in manure and converts them into cellular constituents together with N-P-K resulting in deposition of manure nutrients in biofilm, which can be directly used as a bio-fertilizer.
      Graphical abstract image

      PubDate: 2018-03-07T20:07:05Z
      DOI: 10.1016/j.bcab.2018.02.014
      Issue No: Vol. 14 (2018)
       
  • N- and C-terminal regions of carrot heat shock protein 17.7 can confer
           abiotic stress tolerance to transformed Escherichia coli
    • Authors: Eunhye Ko; Yeh-Jin Ahn
      Pages: 145 - 150
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Eunhye Ko, Yeh-Jin Ahn
      Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that consist of divergent N-terminal and conserved C-terminal regions containing an α-crystallin domain. We introduced the full-length and truncated N- or C-terminal polypeptides of carrot sHsp 17.7 (DcHsp17.7) into Escherichia coli and examined their possible functions in abiotic stress. All three polypeptides increased soluble protein levels and cell viability compared to those of the vector control in stressed E. coli. The heterologous expression of the truncated N-terminal region resulted in the highest levels of stress tolerance under heat, salt, osmotic pressure, and nanomaterial conditions, followed by the full-length DcHsp17.7 and C-terminal region of the protein. When exposed to cold stress, the transformed cell line expressing the full-length DcHsp17.7 showed the highest tolerance levels, followed by the truncated N- and C-terminal regions. Our results suggest that the N- and C-terminal regions of DcHsp17.7 can function independently to increase stress tolerance and that their functional mechanism may be complex, depending on the type of stress presented.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.003
      Issue No: Vol. 14 (2018)
       
  • Extracellular biosynthesis of silver nanoparticles using Streptomyces
           griseoplanus SAI-25 and its antifungal activity against Macrophomina
           phaseolina, the charcoal rot pathogen of sorghum
    • Authors: Rajendran Vijayabharathi; Arumugam Sathya; Subramaniam Gopalakrishnan
      Pages: 166 - 171
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Rajendran Vijayabharathi, Arumugam Sathya, Subramaniam Gopalakrishnan
      Streptomyces griseoplanus SAI-25 isolated from rice rhizospheric soils with previously demonstrated insecticidal activity is currently characterized for silver nanoparticle synthesis using its extracellular extract. The synthesized particles showed the characteristic absorption spectra of silver nanoparticles at 413–417 nm. Spectral analysis by FTIR confirmed the presence of alcohols, amines, phenols and protein in the cell-free extracellular extract of SAI-25. These functional groups could have served dual roles in silver nanoparticle synthesis like reducing and stabilizing agents. Microscopic and spectroscopic analysis such as SEM, TEM, EDAX and XRD has provided the size, shape and composition of the synthesized nanoparticles. DLS and Zeta potential further confirms the size and characteristic negative charges of AgNPs respectively. The observed antifungal activity against charcoal rot pathogen Macrophomina phaseolina shows a base for the development of Streptomyces mediated nanoparticles in controlling this polyphagus pathogen and key role of biopesticides in improving agricultural economy.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.006
      Issue No: Vol. 14 (2018)
       
  • Mathematical modeling of lactic acid fermentation in bioreactor with carob
           extract
    • Authors: Mustafa Germec; Mustafa Karhan; Katherine L. Bialka; Ali Demirci; Irfan Turhan
      Pages: 254 - 263
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Mustafa Germec, Mustafa Karhan, Katherine L. Bialka, Ali Demirci, Irfan Turhan
      In this study, nonlinear sixteen mathematical models including Gompertz (G), generalized Gompertz (GG), modified Gompertz (MG), re-modified Gompertz (RMG), logistic (L), generalized logistic (GL), modified logistic (ML), re-modified logistic (RML), Richards (R), generalized Richards (GR), modified Richards (MR), re-modified Richards (RMR), Stannard (S), Baranyi (B), Weibull (W), and Morgan-Mercer-Flodin (MMF) were applied to fit cell growth, product formation, and sugar consumption of batch lactic acid (LA) fermentation in stirred tank bioreactor with carob extract. To determine the modeling success, root-mean-square-error (RMSE), mean-absolute-error (MAE), R 2 , slope, bias factor (BF), and accuracy factor (AF) were used. Results indicated that the best model for cell growth was MMF model (RMSE=0.24 g/L, MAE=0.16 g/L, R 2 =1.00, Slope=1.06, BF=1.04 and AF=1.16). For product formation, the best models selected were RMG (RMSE=1.33 g/L, MAE=1.00 g/L, R 2 =0.99, Slope=0.95, BF=0.94 and AF=1.11) and RMR (RMSE=1.33 g/L, MAE=1.01 g/L, R 2 =0.99, Slope=0.96, BF=0.94 and AF=1.12) models. As for sugar consumption, B model was the best model for estimation of experimental data (RMSE=0.88 g/L, MAE=0.52 g/L, R 2 =1.00, Slope=1.00, BF=1.00 and AF=1.01). Additionally, the most successful models that predict experimental kinetic data were GG, GL, S, and W models. Consequently, the best models selected could be used for more progress of LA production process in bioreactor with carob extract.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.03.018
      Issue No: Vol. 14 (2018)
       
  • Processed gellan gum beads as covalent immobilization carriers
    • Authors: Marwa I. Wahba
      Pages: 270 - 278
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Marwa I. Wahba
      With the aim of employing gellan gum (GG) beads as covalent immobilization carriers, these beads were subjected to two different activation processes. These activation processes differed from one another with respect to the employed polyamine compound as either polyethyleneimine (PEI) or chitosan (CS) was employed. The polyamine treatment step was optimized with the central composite design, and it was shown that the recommended settings for the PEI concentration and the PEI solution pH were 6.15% (w/v) and 8.30, respectively. On the other hand, the optimum CS solution was shown to be a 2.20% (w/v) solution with a pH value of 2.77. The PEI processed GG beads caused the immobilized β-D-galactosidase (β-gal) to exhibit a more acidic pH optimum than that offered when the CS processed beads were employed. Nevertheless, the two immobilized β-gal samples offered similar temperature profiles. They also exhibited comparable operational stabilities where 81.22 ± 2.63% and 85.17 ± 1.78% of their initial activities were preserved throughout the 14th reusability run of the PEI and the CS processed GG beads, respectively.
      Graphical abstract image

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.03.019
      Issue No: Vol. 14 (2018)
       
  • Fermentative production, purification of inulinase from Aspergillus
           terreus MTCC 6324 and its application for hydrolysis of sucrose
    • Authors: Deepali B. Magadum; Ganapati D. Yadav
      Pages: 293 - 299
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Deepali B. Magadum, Ganapati D. Yadav
      The production of inulinase was carried out with Aspergillus terreus MTCC 6324. To enhance the production of inulinase, fermentation media was optimized by one factor at a time and response surface methodology (RSM), which increases the activity of inulinase by 3 folds. Inulinase was partially purified with ultrafiltration method with 67.7% yield. The purified inulinase was then immobilized by entrapment method in calcium alginate beads with retention of 42% of original activity and employed for hydrolysis of sucrose. After optimization of reaction conditions such as speed of agitation, catalyst loading and temperature, the conversion of sucrose hydrolysis reaction was increased up to 91.57%.
      Graphical abstract image

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.03.020
      Issue No: Vol. 14 (2018)
       
  • Fusarium fujikuroi: A novel source of metabolites with herbicidal activity
    • Authors: Jair J. Daniel; Giovani L. Zabot; Marcus V. Tres; Ricardo Harakava; Raquel C. Kuhn; Marcio A. Mazutti
      Pages: 314 - 320
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Jair J. Daniel, Giovani L. Zabot, Marcus V. Tres, Ricardo Harakava, Raquel C. Kuhn, Marcio A. Mazutti
      This study presents the isolation and identification of a fungus (Fusarium fujikuroi) from the Brazilian Pampa biome that is able to produce phytotoxic secondary metabolites with herbicidal activity. Before selecting such promising fungus, 132 microorganisms were isolated from weed plants with infections symptoms. Each sample of isolated material was used in submerged fermentation to produce a fermented broth. Thereafter, the cells were separated by centrifugation and the supernatant was filtered. Each filtered sample (culture filtrate) was used to evaluate the herbicidal activity in the bioassays with two target plants: Cucumis sativus and Sorghum bicolor. The selection criteria consisted of analyzing germination in pre-emergence, phytotoxicity, plant height and root length in post-emergence, and lesions in detached leaf-punctured assay. The pre-emergence was the primary screening, where 11 samples of culture filtrate provided 100% inhibition of germination of both plants. These samples underwent a secondary screening, which the sample SO210 presented 25% phytotoxicity, 40% reduction of plant height, 28% reduction of root length, and necrosis and chlorosis in 70% of foliar area. The microorganism coded as SO210 was identified as a strain of Fusarium fujikuroi. Up to now, studies that attribute herbicidal activity to this fungus are scarce in the literature. Therefore, this work shows a potential activity of metabolites produced by this fungus toward the inhibition of germination and development of plant weeds.
      Graphical abstract image

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.04.001
      Issue No: Vol. 14 (2018)
       
  • Purification and characterization of xylanase isoenzymes from red palm
           weevil Rhynchophorus ferrugineus
    • Authors: Magda A. Mohamed; Manal M.E. Ghanem; Ahmed M. Abd-Elaziz; Ibrahim M. Shams-Eldin
      Pages: 321 - 327
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Magda A. Mohamed, Manal M.E. Ghanem, Ahmed M. Abd-Elaziz, Ibrahim M. Shams-Eldin
      Xylanase activity in the guts of Rhynchophorus ferrugineus during larval development had been investigated. The activity in the guts from the 5th to the 12th instars ranged from 12.5 to 67 U/gut with specific activity ranged from 50 to 124 U/mg protein. The 10th instars larvae had the highest enzyme activity. Purification of two predominant xylanase isoenzymes was performed by gel filtration on Sephacryl S-200 and chromatography on DEAE-Sepharose. XyI and XyII had specific activities of 468.6 and 402 U/mg proteins, native molecular weights of 25 and 42 kDa, respectively. They had monomeric forms, showed identical optimum activity at pH 5.5 and 40 °C. XyII exhibited higher thermal stability and activation energy than XyI. Inhibition by Hg2+, Cu2+, EDTA and stimulation by dithiol-reducing agents revealed the presence of at least one sulfhydryl group in the active site of the enzyme and they were metalloenzyme. They exhibited high specificity towards natural xylans. They produced xylotriose, xylotetraose and xylopentaose as the main hydrolysis products of beechwood xylan. The results were compared with those previously recorded for different insect species.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.04.002
      Issue No: Vol. 14 (2018)
       
  • Whey protein isolate for the preparation of covalent immobilization beads
    • Authors: Marwa I. Wahba; Tarik N. Soliman
      Pages: 328 - 337
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Marwa I. Wahba, Tarik N. Soliman
      Whey protein isolate (WPI) was employed, for the first time, to activate carrageenan (Car) beads for the covalent immobilization of the Aspergillus oryzae β-D-galactosidase (β-gal). These Car beads were subjected to a WPI treatment step followed by a glutaraldehyde (GA) treatment step in order to enable such covalent immobilization. The WPI treatment was optimized via the Box-Behnken Design (BBD). The BBD anticipated that treating the Car beads with a 2.36% WPI solution of pH 5.25 for 7.04 h would allow for the attainment of an immobilized β-gal's activity recovery percent of 34.43%. A verification experiment was accomplished while employing the abovementioned conditions and an immobilized β-gal's activity recovery percent of 34.80 ± 1.11% was attained. It was also shown that the immobilization of β-gal onto the GA-WPI treated Car beads did not alter the enzyme's optimum temperature or optimum pH. Moreover, a reusability study was conducted and 93.84 ± 0.72% of the immobilized β-gal's initial observed activity was preserved during the 13th reusability cycle.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.04.003
      Issue No: Vol. 14 (2018)
       
  • Tissue-specific heat-induced changes in the expression of ncRNA fragments
           in Brassica rapa plants
    • Authors: Boseon Byeon; Andriy Bilichak; Igor Kovalchuk
      Pages: 338 - 356
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Boseon Byeon, Andriy Bilichak, Igor Kovalchuk
      Non-coding RNA fragments (ncRFs) represent a large spectrum of short ncRNAs processed from precursor ncRNAs such as tRNA, rRNA, snoRNA and several others. Several recent papers suggest that ncRFs are processed in a specific manner in different tissues or in response to stress. Here, we analyzed alterations in the expression of ncRFs in different Brassica rapa tissues in response to heat stress. Specifically, we analyzed the abundance of tRNA fragments (tRFs), rRNA fragments (rRFs), snoRNA fragments (snoRFs) and snRNA fragments (snRFs). The most significant changes in response to heat stress were observed in tRNAGlu and tRNAAsp. Whereas the number of reads mapping to the former one dropped drastically in all tissues but the apical meristem and pollen, the number of reads mapping to the latter significantly increased. Analysis of tRFs showed that three isoacceptors, tRF5′Asp(GUC), tRFGly(UCC) and tRFPseudo(UCC) were severely underrepresented in heat-stressed tissues. Heat also induced changes in the processing of ncRFs from precursor molecules; a bias towards the processing of ncRFs from the 3′ end was even more prominent after the heat stress application. The analysis of predicted targets of ncRFs revealed an enrichment in several groups of genes involved in the response to various stresses. To conclude, our work showed that heat stress results in tissue-specific changes in the expression of ncRFs in Brassica rapa and suggested a tissue-specific regulation of various mRNAs and pathways in response to heat.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.03.024
      Issue No: Vol. 14 (2018)
       
  • Possible correlation among osmophilic bacteria, levan yield, and the
           probiotic activity of three bacterial honey isolates
    • Authors: Walaa A. Abdel Wahab; Shireen A.A. Saleh; Eman A. Karam; Nahla M. Mansour; Mona A. Esawy
      Pages: 386 - 394
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Walaa A. Abdel Wahab, Shireen A.A. Saleh, Eman A. Karam, Nahla M. Mansour, Mona A. Esawy
      Three bacterial strains were isolated from different honey sources. They were identified based on 16S rRNA as Bacillus endophyticus SH, Bacillus subtilis WA and Bacillus subtilis MO. All the isolates had the ability to yield levan in the presence of 80 g/L sucrose with a degree of variation. The osmotic tolerance capacity of the three honey isolates was examined on sucrose, NaCl, sea water, NaCl/ sucrose. The results categorized the isolates as moderate halophiles where they reported their maximum growth between (4–8%) NaCl or (20–50%) sea water, also their highest growth was obtained at 4% sucrose and they were tolerant to sucrose stress till 20%. The probiotic studies revealed that the vegetative cells of the three isolates were highly resistant to severe acidic and alkaline pHs and to different concentrations of bile salt and pancreatic enzymes with variant degrees. Isolates safety was proved by negative blood hemolysis, an absence of hemolytic cytotoxin K (cytK) and non-hemolytic enterotoxin (nheA, nheB, and nheC) genes. In addition, the isolates were sensitive to the tested antibiotics including streptomycin, ampicillin, novobiocin, nalidixic acid, vancomycin, kanamycin, ciprofloxacin, and oxytetracyclin. Finally, they reported varying antimicrobial activities against different pathogens causing human problems. This study suggested B. endophyticus SH for the first time as a safe probiotic bacterium, also recommended the three isolates for using in different aspects such as food supplement.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.04.006
      Issue No: Vol. 14 (2018)
       
  • Stress tolerance and plant growth promotion potential of Enterobacter
           ludwigii PS1 isolated from Seabuckthorn rhizosphere
    • Authors: Diskit Dolkar; Phuntsog Dolkar; Stanzin Angmo; O.P. Chaurasia; Tsering Stobdan
      Pages: 438 - 443
      Abstract: Publication date: Available online 19 April 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Diskit Dolkar, Phuntsog Dolkar, Stanzin Angmo, OP Chaurasia, Tsering Stobdan
      Plant growth promotion by microbial inoculants is affected by environmental factors. The study was therefore aimed at developing microbial inoculants for promoting tomato growth in regions experiencing temperature, pH and salt stressed conditions. Enterobacter ludwigii PS1 capable of solubilizing insoluble inorganic phosphate was isolated from Seabuckthorn rhizosphere growing in the Indian trans-Himalaya. PS1 showed ability to solubilize insoluble phosphate under different stress conditions viz. 4–44°C temperature, 1–5% salt concentration and 4–12pH range. The isolate exhibited multiple plant growth promoting traits viz. auxin (24.3mgL-1), siderophore (79%) and hydrogen cyanide (0.21O.D at 625nm) production. Tomato seed bacterization resulted in 25% and 37% increase in shoot and root length. Inoculation of tomato seedling with PS1 promoted plant growth in pot trial experiments in trans-Himalayan (34°08ʹ0.2ʺ N, 77°34ʹ0.3ʺ E, 3340m asl) condition. Increase in fruit yield was 15% in open and 27% in shade net condition. E. ludwigii PS1 with phosphate-solubilizing ability under stress conditions appears to be attractive for exploring its plant growth-promoting activities towards development of microbial inoculants in stressed regions.

      PubDate: 2018-04-25T00:01:16Z
      DOI: 10.1016/j.bcab.2018.04.012
      Issue No: Vol. 14 (2018)
       
  • Comparative pot studies of chitosan and chitosan-metal nanocomposites as
           
    • Authors: Pawan Kaur; Joginder Singh Duhan; Rajesh Thakur
      Pages: 466 - 471
      Abstract: Publication date: April 2018
      Source:Biocatalysis and Agricultural Biotechnology, Volume 14
      Author(s): Pawan Kaur, Joginder Singh Duhan, Rajesh Thakur
      Fusarium wilt is a seed borne and soil borne disease of chickpea caused by Fusarium oxysporum f. sp. ciceri (FOC). In this work, chitosan and their nanocomposites were evaluated as antifungal agents against FOC, in vitro as well as in vivo. Among these, chitosan copper oxide nanocomposites (Ch-CuO) and chitosan-zinc oxides nanocomposites (Ch-ZnO) were found to be the most effective against FOC at all recommended concentrations i.e. 50, 100 and 200 µg/ml. Chitosan nanoparticles (Ch) and chitosan-silver nanocomposites (Ch-Ag) were found to be moderately effective but more efficient than standard fungicide i.e. copper-oxy-chloride (CuOCl). Based on in vitro results, 100 µg/ml concentration of all nanoformulations (NFs) was selected for in vivo studies in potted plants. The highest wilt disease reduction was observed in Ch-CuO (46.67%) followed by plants treated with Ch-ZnO (40%) as moderately effective, while Ch-Ag and Ch caused only 33.33% reduction in wilt incidence as less effective. All nanoformulations showed good antifungal efficacy and inhibited the pathogen as well as found to promote the growth of chickpea plants as compared to untreated plants.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.04.014
      Issue No: Vol. 14 (2018)
       
  • Comment on In vivo assessment of possible probiotic properties of Bacillus
           subtilis and prebiotic properties of levan
    • Authors: Fereshteh Ansari; Hadi Pourjafar
      Abstract: Publication date: Available online 29 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Fereshteh Ansari, Hadi Pourjafar


      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.019
       
  • Biodelignification of some agro-residues by Stenotrophomonas sp. CFB-09
           and enhanced production of ligninolytic enzymes
    • Authors: Folasade M. Olajuyigbe; Cornelius O. Fatokun; Oluwadara M. Oyelere
      Abstract: Publication date: Available online 28 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Folasade M. Olajuyigbe, Cornelius O. Fatokun, Oluwadara M. Oyelere
      Biological delignification is an environmental friendly pretreatment method for enhanced enzymatic hydrolysis of lignocellulosic biomass and fermentation processes. In an attempt to optimize delignification process which is crucial for effective biofuel production from agro-residues, this study investigated biodelignification of some agro-residues (coconut shell {CS}, coconut husk {CH}, palm kernel shell {PKS}, oil palm empty fruit bunch {OPEFB} and sawdust {SD}) by Stenotrophomonas sp. CFB-09 under submerged fermentation conditions. Initial lignin and residual lignin contents of each agro-residue were determined at the beginning and end of 216h biodelignification period. Production of ligninolytic enzymes was monitored at 12h intervals. Effects of nitrogen sources, temperature and pH on production of ligninolytic enzymes were determined over the biodegradation period. Remarkably, biodelignification of the agro-residues was in the range of 41 to 55%. Laccase had highest yield (31 U/mg) on CH, PKS and OPEFB. Lignin peroxidase production (381 U/mg) was maximum on OPEFB, and manganese peroxidase had highest yield (53 U/mg) on PKS. Surprisingly, there was appreciable production of ligninolytic enzymes at higher temperatures of biodegradation with 40–55% yield at 80°C. Maximum enzyme production was achieved at 40°C, pH 8.0 with ammonium nitrate as nitrogen source. Results provide deeper insights into biodelignification of agro-residues and demonstrate potential of Stenotrophomonas sp. CFB-09 for use in biodegradation of agro-residues, especially in pretreatment of lignocellulosic biomasses for production of biofuel and other value-added products.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.016
       
  • Antioxidant, Antimicrobial and Antibiotic Resistance Modifying Effect of
           Heliotropium indicum
    • Authors: Parvaze Ahmad Wani; Arowolo Mojisola Tolu; Shazia Wahid
      Abstract: Publication date: Available online 28 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Parvaze Ahmad Wani, Arowolo Mojisola Tolu, Shazia Wahid
      The present study is based on the determination of phytochemical, antimicrobial, anti-oxidant and antibiotic resistance modifying activities of soil grown Heliotropium indicum. The phytochemical screening showed the presence of Tannin, Oxalate, Phytate, Terpenoids, Trypsin inhibitors, Phenol, Carotenoids, Carotene, Xanthophyll, Flavonoid, Saponin, and Alkaloids. Acetone extracts by paper disc and open well method showed highest antimicrobial activity against different pathogens which was followed by ethanol extract. Lowest MIC was also shown by acetone, followed by ethanol. Fractional inhibitory concentration was also determined, in which acetone and ethanol generally showed synergistic effect compared to aqueous extract which showed mostly additive effect. As the concentration of the plant extract increased, antioxidant activity also increased. Acetone was able to show highest activity of antioxidants compared to other solvents. Based on the above facts, it is concluded that the extract can be used as an antimicrobial, antioxidant and antibiotic resistance modifying agent.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.018
       
  • Phytochemical screening, free radical scavenging and antimicrobial
           potential of Chromolaena odorata leaf extracts against pathogenic
           bacterium in wound infections– a multispectrum perspective
    • Authors: Kavitha Vijayaraghavan; Johanna Rajkumar; Mohamed Ali Seyed
      Abstract: Publication date: Available online 25 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Kavitha Vijayaraghavan, Johanna Rajkumar, Mohamed Ali Seyed
      The chronic bed ridden patients acquire infections are the main source of alarming morbidity and mortality as their correlation with fomites. Fomites are reservoirs of drug-resistant pathogens found in the unhygienic clinical environment, which can infect patients and leading to high nosocomial infection rates and use of antimicrobial drugs. The use of and search for drugs and dietary supplements derived from plants have accelerated various therapeutic applications and obtaining new antimicrobial agents from natural resources is necessary to develop alternative drugs for safe and cost-effective health care. Hence, the objective of this research was to perform a phytochemical analysis on Chromolaena odorata and to study the antioxidant and antimicrobial activities of the ethanolic and aqueous extracts of its leaves. In this regard, phytochemicals obtained from C.odorata was investigated for their radical scavenging activity using 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The phytochemical analysis revealed the presence of active ingredients such as glycosides, steroids, saponins, phenols, flavonoids, terpenoids, and tannins were categorized either as primary metabolites, such as chlorophyll, proteins, and amino acids, or as secondary metabolites, which include terpenoids and alkaloids. The ethanolic leaf extract showed a higher antioxidant activity and antibacterial properties than the aqueous extract exhibited. C. odorata leaf extracts of both ethanoloic and aqueous were subjected to an antibacterial study using the agar diffusion method. Our antibacterial results showed that the ethanolic extract could inhibit to a certain extent the growth of various human pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Escherichia coli. In summary, the present study highlights the significance of C. odorata leaf extracts for their antioxidant and antibacterial potentials, which explain the traditional use of the plant in animal and human medicine and cost-effective health care.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.014
       
  • Enhancement of biocontrol potential of biocompatible bovine serum albumin
           (BSA) based protein nanoparticles loaded bacterial chitinase against major
           plant pathogenic fungi Alternaria alternta
    • Authors: G. Narendrakumar; S. Karthick Raja Namasivayam; M. Manikanta; Mishal Saha; Tirana Dasgupta; N. Divyasri; Ch. Anusha; B. Arunkumar; T.V. Preethi
      Abstract: Publication date: Available online 24 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): G. Narendrakumar, S. Karthick Raja Namasivayam, M. Manikanta, Mishal Saha, Tirana Dasgupta, N. Divyasri, Ch. Anusha, B. Arunkumar, T.V. Preethi
      Recently, principles of nanoscience and nanotechnology has being embraced in the field of agriculture rather than medicine and health care which has the potential to revolutionize modern day agriculture by effectively controlling insect pests and disease causing phytopathogenic microorganisms. In the present study, biocontrol potential of bovine serum albumin nanoparticles (BSA Nps) loaded extra cellular chitinase (BSA Np-CHS) produced by Serratia marcescens SU05 was studied against phytopathogenic fungi Alternaria alternate. Chitinase was extracted from the chitinase production medium that produced by optimizing various nutrient and process conditions adopting Taguchi method (37°C temperature, 7pH, 50rpm and 1.5 inoculum) shows a extended production of enzyme. Extracted enzyme was purified by ammonium sulphate precipitation and column chromatography separation. Purified enzyme thus obtained was loaded with BSA Nps by cocoeravation method. The method for the preparation of BSA Np-CHS conjugate was optimized by various parameters. Nano enzyme conjugate thus prepared using optimal condition reveals spherical nanosphere with the size range of 110–120nm and changes in the functional group that was studied by Fourier transform infrared spectroscopy (FTIR) analysis. Biocontrol efficacy of the prepared BSA Np-CHS nano enzyme conjugate against tested fungal strain was studied by determination of fungal biomass and fungal hyphal fragments damage under in vitro condition. BSA Np-CHS nano enzyme conjugate brought about effective reduction of fungal biomass and high rate of fungal hyphae fragments damage in all the tested concentration. Further study will helpful to formulate, apply the nanoformulation under field trails for effective control of phytopathogenic fungi.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.015
       
  • Four novel strains of cellulolytic symbiotic bacteria isolated and
           characterized from GI tract of marine fishes of various feeding habits
    • Authors: Asha Augustine; Imelda Joseph
      Abstract: Publication date: Available online 17 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Asha Augustine, Imelda Joseph
      Selected marine fishes with different feeding habits were screened for the presence of symbiotic cellulolytic bacteria in their gut. Four cellulolytic species of symbiotic bacteria were isolated from GI tract of marine fishes namely Carangoides praeustus, Filimanus similis, Sardinella longiceps and Sillago sihama. The strains were identified after polyphasic phenotypic and genotypic (16S rRNA gene) characterization as Bacillus subtilis strain TCPC1, Vibrio alginolyticus strain CFSS2C2, Pseudomonas stutzeri strain KSLS4C3 and Klebsiella oxytoca strain MSSC4. (Genbank Accession nos.: JN710380, JN710378, JN710377, JN712301).The results indicated the presence of cellulolytic bacteria in GI tract of marine fishes of carnivorous, phytoplanktivorous and omnivorous feeding habits. Cellulolytic activity was the maximum for B. subtilis strain TCPC1 (0.45 mg glucose ml−1) and V. alginolyticus strain CFSS2C2 (0.24 mg glucose ml−1) at 234 h. While P. stutzeri strain KSLS4C3 showed the maximum utilization (0.22 mg glucose ml−1) from 240 to 258 h. K. oxytoca strain MSSC4 (0.47 mg glucose ml−1) showed three peaks during the study. The maximum rate of cellulose utilization was shown by P. stutzeri strain KSLS4C3 (0.05 mg glucose ml−1 medium h−1) followed by K. oxytoca strain MSSC4 (0.03 mg glucose ml−1 medium h−1).

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.009
       
  • AN EVALUATION OF INVITRO AND INVIVO FREE RADICAL SCAVENGING AND
           ANTIOXIDANT POTENTIAL OF ETHANOLIC EXTRACT OF PERGULARIA DAEMIA
    • Authors: G. Sridevi; S. Srividya; K. Sembulingam; Prema Sembulingam
      Abstract: Publication date: Available online 14 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): G. Sridevi, S. Srividya, K. Sembulingam, Prema Sembulingam
      Oxidative stress plays a major role in developing chronic, degenerative, cardiovascular and neurodegenerative diseases caused by excessive production of reactive oxygen species. To combat against this oxidative induced free radical generation, many antioxidant compounds have been explored. In the present study, the antioxidant potential of traditional herb, Pergularia daemia (PD) was explored by invitro and invivo methods. The invitro analysis was done by determining the total antioxidant capacity, DPPH, superoxide and hydrogen peroxide scavenging activity and ferric reducing antioxidant capacity using spectrophotometry. Invivo study was done by pre-treating wistar strain albino rats with ethanolic extract of PD and exposing them to acute noise stress using a pure tone noise from a function generator for 45mins for 1day. The discrete brain regions were dissected and the brain enzymatic and non-enzymatic antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C and lipid peroxidation were analyzed. Results of invitro analysis revealed that PD extract exhibited a potent antioxidant capacity, scavenged the DPPH, superoxide and hydrogen peroxide and reduced the ferric ions to ferrous form in a dose dependent manner. Animals exposed to acute noise stress for 45minutes showed a significant increase in plasma corticosterone and significantly increased the superoxide dismutase, catalase, glutathione peroxidase activity and lipid peroxidation in cerebral cortex, striatum, hippocampus and hypothalamus and decreased the reduced glutathione and vitamin C levels in all the four discrete regions of the brain. PD treatment significantly prevented those acute noise stress induced changes and restored it to normal.

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.007
       
  • Biotechnological valorization of pineapple stem for pectinase production
           by Bacillus subtilis BKDS1: Media formulation and statistical optimization
           for submerged fermentation
    • Authors: Bijesh Kavuthodi; Denoj Sebastian
      Abstract: Publication date: Available online 12 May 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Bijesh Kavuthodi, Denoj Sebastian
      Agro-residues are mainly comprised of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. India is one of the largest producers of pineapple and along with the expanding pineapple production, associated agro-wastes are also increasing proportionally. In this background, the present study has been endeavored to accomplish valorization of pineapple stem for the economical production of the enzyme, pectinase by Bacillus subtilis BKDS1 using pineapple stem extract (PSE) medium. The results revealed that, the effective concentration of the extract towards producing maximum pectinase enzyme was found to be 12.5% in distilled water. Enhancement of pectinase production in terms of media optimization has been attempted using Plackett-Burman design (PBD) and response surface methodology (RSM). Out of nine variables screened by PBD, only four variables; yeast extract, CaCl2, CaCO3 and inoculum have been selected to affect the pectinase production significantly. The single and interactive effects of all the selected variables have been analyzed using Central composite design (CCD) of RSM. The data revealed that, optimized medium produces a higher concentration of enzyme than unoptimized medium. Further, the efficiency of modified PSE medium for the production of pectinase by submerged fermentation (Smf) has confirmed in laboratory scale fermenter. It has also been found that, enzyme production is achieved at a faster rate in fermenter compared to shaker flasks. Thus, the study as whole highlights the fact that, pineapple stems can be successfully employed in the production of the industrially important enzyme, pectinase using B. subtilis BKDS1. This will in turn, can deliver a lot towards the proper management of agro-wastes and also ensure a better cleaner environment.
      Graphical abstract image

      PubDate: 2018-05-31T14:14:53Z
      DOI: 10.1016/j.bcab.2018.05.003
       
  • Root colonization by the endophytic fungus Piriformospora indica improves
           growth, yield and piperine content in black pepper (Piper nigurm L.)
    • Authors: K.N. Anith; S. Aswini; Shilpa Varkey; N.V. Radhakrishnan; Deepa S. Nair
      Abstract: Publication date: Available online 19 March 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): K.N. Anith, S. Aswini, Shilpa Varkey, N.V. Radhakrishnan, Deepa S. Nair
      Plant growth and yield characteristics of black pepper (Piper nigrum L.) on inoculation with the root endophytic fungus Piriformospora indica are reported. Experiments were carried out using plants raised from the lateral branches of black pepper which develop into miniature plants known as “bush pepper” that bear fruits in the same year of planting. P. indica (Pi) inoculated plants put forth more number of leaves and leaf area per plant compared to the control plants throughout the period of the experiment. There was significant difference between the inoculated and uninoculated plants with respect to the chlorophyll a and total chlorophyll content of leaf tissues. Early flowering and spike setting was observed in plants inoculated with Pi. The total fresh and dry weights of berries harvested from the Pi inoculated plants were significantly higher than that from the control plants. Inoculation with the fungus also increased the total oleoresin and piperine content in the berries.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.012
       
  • SELENORHIZOBACTERIA: AS BIOFORTIFICATION TOOL IN SUSTAINABLE AGRICULTURE
    • Authors: Priyanka J. Patel; Goral R. Trivedi; Rupal K. Shah; Meenu Saraf
      Abstract: Publication date: Available online 17 March 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Priyanka J. Patel, Goral R. Trivedi, Rupal K. Shah, Meenu Saraf
      Selenium biofortification in crops aims to either increase the accumulation of selenium in edible plants or to increase their bioavailability. It is one of the solutions for globally increasing hidden hunger for essential micronutrients. Plant growth promoting rhizobacteria are advantageous soil bacteria that inhabit plant roots and increase plant growth through various mechanisms in different ways. The motion of selenium across soil, crop and environment interfaces is thus of crucial importance for gain in human selenium status. This review gives an overview of microbial enhancement of selenium as beneficial element for plants, significance of selenium to human health, selenium response in soil crop system, selenium as plant protector against abiotic stresses and the possible approaches to enhancing selenium concentration through use of microorganisms (selenorhizobacteria) as biotechnological tools for increasing plant nutrition and quality.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.013
       
  • Screening and production of lipase from fungal organisms
    • Authors: Kiptoo Geoffry; Rajeshwara N. Achur
      Abstract: Publication date: Available online 16 March 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Kiptoo Geoffry, Rajeshwara N. Achur
      Lipases (triacylglycerol acylhydrolases, (E.C. 3.1.1.3) are a class of enzymes endowed with an ability to catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. These are industrially significant, particularly microbial lipases. Among several diverse sources, lipases from fungal origin are much embraced owing to their high stability. These lipases are directly screened majorly by the plate assay technique involving various preferred and specific substrates and indirectly by appropriately growing the strains in liquid medium followed by analysing the activity of the filtrates. Production of fungal lipase is majorly by using solid state and submerged fermentation processes with considerable variation in their operational conditions, that are influenced by physico-chemical factors and which plays a major role in the optimum lipase production. Further, the immobilized fungal cells have also been employed for lipase production using various support materials. Keeping in view of all these aspects, this review focuses on the screening, production and utility of lipases obtained from prominent fungal organisms.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.009
       
  • Particle bombardment-mediated co-transformation of the Cht-2 gene in wheat
           and the associated changes in defense mechanisms in transgenic plants
           infected with Fusarium graminearum
    • Authors: Hanan A. Hashem; Raifa A. Hassanein; Ashraf H. Fahmy; Ahmed S. Ibrahim; Osama M. El Shihyh; Ebtesam A. Qaid
      Abstract: Publication date: Available online 14 March 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Hanan A. Hashem, Raifa A. Hassanein, Ashraf H. Fahmy, Ahmed S. Ibrahim, Osama M. El Shihyh, Ebtesam A. Qaid
      Fusarium graminearum is a major global pathogen of cereals and is considered the main causal agent of Fusarium head blight disease in wheat. Infection with F. graminearum causes a significant reduction in crop yield and quality; therefore, it is very important to improve wheat pathogen resistance. In the present study, the plasmid pAHCht-2 harboring the rice chitinase (Cht-2) gene for pathogen resistance and the plasmid pAB6 containing the gus reporter and bar selectable marker genes were used for genetic transformation of immature embryo-derived calli of the Egyptian wheat cultivar Giza 164 using particle bombardment. The presence and integration of transgenes were assessed by PCR analysis using specific primers for the Cht-2, bar and gus genes. The incorporation of the Cht-2 gene into the genome of the transformants was confirmed by dot-blot analyses. The transformation efficiency (number of transgenic plants/number of embryos) was 6.01%. Additionally, associated changes in defense mechanisms in the transgenic plants were investigated. The results showed variations in biochemical characteristics between transgenic and non-transgenic wheat lines (cv. Giza 164). The transgenic plants had significantly decreased total protein content, phenolic compounds and antioxidant enzyme activities (peroxidase and catalase), and significantly increased phenylalanine ammonia lyase and chitinase activities compared with non-transgenic plants under biotic stress conditions caused by F. graminearum infection. Our results show that activating a specific program of gene expression related to particular environmental stress conditions can reduce the cost of the stress on plant metabolism.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.007
       
  • In Vitro Propagation of Sugarcane (Saccharum officinarum L.) Variety
           C86-56 through Apical Meristem
    • Authors: Mulugeta Hailu Redae; Teklit Gebregiorhis Ambaye
      Abstract: Publication date: Available online 13 March 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Mulugeta Hailu Redae, Teklit Gebregiorhis Ambaye
      Sugarcane (Saccharum officinarum L.) is monocotyledonous crop plant that mostly propagates through conventional methods. However, conventional propagation lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize in vitro micropropagation protocol for sugarcane variety (C86-56) through apical meristem. The variety was cultured on MS medium supplemented with different concentrations of growth regulators on shoot initiation, multiplication, rooting and acclimatization stages. The results showed that significant difference in response to the various hormonal treatments with regard to the parameters measured. For initiation stage, the best performance was observed on MS medium supplemented 1.0mg/l of BAP. On the other hand, multiplication stage was best on MS media enriched with 2.0mg/l BAP + 1.0mg/l NAA as manifested in terms of a mean number of shoots and mean shoot length. With regard to root induction, the best-rooting response in terms of mean root number and mean root length was achieved best on 1/2 MS media enriched with 2.0mg/l NAA + 0.5mg/l BAP. Survival rate during acclimatization was best on coco peat media at a rate of 98%.

      PubDate: 2018-03-19T21:54:32Z
      DOI: 10.1016/j.bcab.2018.03.005
       
  • The potential of selected purple nonsulfur bacteria with ability to
           produce proteolytic enzymes and antivibrio compounds for using in shrimp
           cultivation
    • Authors: Natchapat Seangtumnor; Duangporn Kantachote; Phithaya Nookongbut; Ampaitip Sukhoom
      Abstract: Publication date: Available online 22 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Natchapat Seangtumnor, Duangporn Kantachote, Phithaya Nookongbut, Ampaitip Sukhoom
      This study was aimed to screen 22 purple nonsulfur bacteria (PNSB) isolates with the ability to secrete proteolytic enzymes and antivibrio compounds, including affecting factors on proteolytic enzyme production of the selected PNSB. Use of overlay diffusion method under aerobic dark conditions found that only 12 PNSB isolates (54.55%) were able to inhibit shrimp pathogenic Vibrio spp.; while 18 isolates (81.82%) could liquefy gelatin under conditions of aerobic dark and microaerobic light. Twenty-time freeze-dried culture supernatant concentrates collected from 12 PNSB grown under the microaerobic light conditions, with the use of agar well diffusion found that only strain PS342 was capable to inhibit all 6 tested shrimp pathogenic vibrios. Among 18 proteolytic PNSB, only 5 strains showed high activity of gelatin liquefaction and this included strain PS342. The strain PS342 was identified using 16S rRNA gene as Rhodovulum sulfidophilum. Glutamate-malate (GM) medium supplemented with 1.5% NaCl was the most suitable medium by giving µmax as 0.336h-1. Use of central composite design, the maximal proteolytic activity was an average of 14.52 unit/ml in the suitable medium containing 1% gelatin under aerobic-dark conditions with the optimal speed at 150rpm. The results of the verify test showed that optimum conditions (pH 7.90, 1.30% NaCl and 29.50°C) for the proteolytic activity (15.40 unit/ml) were very close to real conditions for shrimp cultivation. R. sulfidophilum PS342 has the potential to be used in shrimp cultivation for its activities as a good producer of both proteolytic enzymes and antivibrio compounds.

      PubDate: 2018-02-26T18:34:24Z
      DOI: 10.1016/j.bcab.2018.02.013
       
  • Effect of ginger endophyte Rhizopycnis vagum on rhizome bud formation and
           protection from phytopathogens
    • Authors: C. Anisha; P. Jishma; V. Sasi Bilzamol; E.K. Radhakrishnan
      Abstract: Publication date: Available online 22 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): C. Anisha, P. Jishma, V. Sasi Bilzamol, E.K. Radhakrishnan
      Most of the microorganisms residing within the plants are likely to be beneficial to the host plants as they inhibit invasion of phytopathogens and produce plant growth regulators. In the current study, endophytic fungal isolate from Zingiber officinale Rosc. with antagonism to soft rot pathogen Pythium myriotylum was investigated for its broad antiphytopathogenic properties. The crude extract prepared from the organism was found to have activity against various phytopathogens and was confirmed by scanning electron microscopic analysis. The isolate was identified as Rhizopycnis vagum by ITS sequencing. Remarkably, during the rhizome protective studies, the organism was found to prevent P. myriotylum infection in ginger with associated enhancement in germination and bud development. Hence the result is indication of role of endophytic fungi as highly promising and broad spectrum plant probiotic agent.

      PubDate: 2018-02-26T18:34:24Z
      DOI: 10.1016/j.bcab.2018.02.015
       
  • Microwave assisted solvent-free synthesis of n-butyl propionate by
           immobilized lipase as catalyst
    • Authors: Kalpesh V. Bhavsar; Ganapati D. Yadav
      Abstract: Publication date: Available online 21 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Kalpesh V. Bhavsar, Ganapati D. Yadav
      Operational simplicity, solvent free media, potential reusability of catalyst and wide functional group tolerance are the advantages for solvent free synthesis under microwave irradiation technique. In the current work, n-butyl propionate was synthesized by esterification of propionic acid with n-butanol using different immobilized enzymes under microwave irradiation vis-à-vis conventional heating. Commercially available lipases such as Lypozyme RM IM, Lypozyme TLIM and Novozym 435 (Candida antarctica lipase B) were screened for n-butyl propionate synthesis from propionic acid and n-butanol for which Novozym 435 showed the best catalytic activity. n-Butyl propionate is widely used in flavour, painting, enamels, appliance coating and similar processes. In 8h 92% conversions were achieved at 60°C with 1:12 mol ratio of propionic acid to n-butanol under microwave irradiation. Systematic studies were done to understand mechanism and rate. In the Lineweaver-Burk plot the lines are intersecting at a point, showing that the reaction follows ternary complex mechanism with inhibition by n-butanol.
      Graphical abstract image

      PubDate: 2018-02-26T18:34:24Z
      DOI: 10.1016/j.bcab.2018.02.012
       
  • Expression profile of phenylalanine ammonia-lyase (PAL) and phenolic
           content during early stages of graft development in bud grafted Hevea
           brasiliensis
    • Authors: Auraiporn Prabpree; Porntip Sangsil; Charassri Nualsri; Korakot Nakkanong
      Abstract: Publication date: Available online 16 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Auraiporn Prabpree, Porntip Sangsil, Charassri Nualsri, Korakot Nakkanong
      Phenylalanine ammonia lyase (PAL) is a key enzyme in the phenylpropanoid pathway responsible for the biosynthesis of many secondary metabolites, such as anthocyanins, flavanols, and lignins. This study investigated the effect of grafting on vegetative growth, PAL expression and phenolic content of RRIM 600 and RRIT 251 bud grafted on different rubber rootstocks: for homografts (RRIM 600/RRIM 600 and RRIT 251/RRIT 251) and heterografts (RRIM 600/RRIM 623, RRIM 600/Clone#1, RRIM 600/Clone#2) (RRIT 251/RRIM 623, RRIT 251/Clone#1, RRIT 251/Clone#2) at 0, 7, 14, 21 and 28 days after bud grafting. For the RRIM 600 scion, the rootstock that provided the highest growth rate was Clone#1 while RRIT 251 showed uniform growth on different graft combinations. The results indicate that grafting alters PAL gene expression. PAL transcripts at higher levels during the early stages of grafting development especially 7 days after grafting. However, no significant differences were detected in the phenolic content at different post-grafting times. These findings suggest that the expression of the PAL gene is related to grafting compatibility in grafted H. brasiliensis.

      PubDate: 2018-02-18T18:09:00Z
      DOI: 10.1016/j.bcab.2018.02.010
       
  • Immobilization of the green microalga Botryococcus braunii in polyester
           
    • Authors: Néstor D. Giraldo Calderón; Kenny C. Díaz Bayona; Lucía Atehortúa Garcés
      Abstract: Publication date: Available online 13 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Néstor D. Giraldo Calderón, Kenny C. Díaz Bayona, Lucía Atehortúa Garcés
      Botryococcus braunii is a renowned source of biomass, lipids and hydrocarbons for biofuel production. However, this microalga also produces exopolysaccharides (EPS) which might be used industrially. The artificial immobilization of B. braunii has proven to influence its growth and metabolite yield. In this work, B. braunii was immobilized using 3g/L of polyester wadding, a recyclable material no reported before as fixing matrix for this microalga. This inexpensive polymer was non-toxic to the cells and allowed their fixing during 2 months. After 24 days, the final biomass yield (g/L) was statistically higher (P ˂ 0.05) in immobilized (1.05 ± 0.05) than in suspended cultures (0.734 ± 0.003). The final EPS yield (g/L) was also higher in immobilized (0.094 ± 0.008) than in the suspended cultures (0.077 ± 0.004). In both cases, the sugar composition of the EPS (mainly 71.73mol% galactose) and the profile of fatty acids were the same.
      Graphical abstract image

      PubDate: 2018-02-18T18:09:00Z
      DOI: 10.1016/j.bcab.2018.02.006
       
  • Biofuels: Production of fungal-mediated ligninolytic enzymes and the modes
           of bioprocesses utilizing agro-based residues
    • Authors: Saroj Paramjeet; P. Manasa; Narasimhulu Korrapati
      Abstract: Publication date: Available online 10 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Saroj Paramjeet, P. Manasa, Narasimhulu Korrapati
      The depletion of fossil fuels and rise in their global energy consumption and demand have had a major impact on the stability of the ecosystem and highlighted the need for efficient, sustainable, and renewable alternative sources of energy. Lignocellulosic biomass-based biofuels are highly advantageous due to their enormous supply in nature. Enzymatic bioconversion of lignocellulose polysaccharides into monomeric sugars has a higher efficiency than traditional chemical modes of action. Fungal-mediated ligninolytic enzymes offer even greater advantages in the bioconversion of lignocelluloses into simple sugars due to their thermostability, activity across a wide range of pH values, high specificity, and minimal by-products. This review, based on recent developments in the field of fungal-derived ligninolytic enzymes, discusses their mechanisms of action along with their production and the modes of bioprocesses involved as well as different techniques, such as heterologous gene expression, mutagenesis, and co-culturing, that enhance production and improve catalytic and stability properties.

      PubDate: 2018-02-18T18:09:00Z
      DOI: 10.1016/j.bcab.2018.02.007
       
  • Preparation of chiral phenylethanols using various vegetables grown in
           Algeria
    • Authors: Manhel Bennamane; Samra Razi; Saoussen Zeror; Louisa Aribi-Zouioueche
      Abstract: Publication date: Available online 6 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Manhel Bennamane, Samra Razi, Saoussen Zeror, Louisa Aribi-Zouioueche
      Ginger root, strawberry tree and mandarin growing in Algeria were evaluated for their ability to stereoselective reduction of prochiral ketones. The reactivity and the enantioselectivity are strongly dependent on the biocatalyst used, and the structure of ketone. High enantioselectivities were observed for some substrates (70–99% ee) especially for the bioreduction of acetophenone 1, p-chloroacetophenone 2, tetralone 5, thiochromanone 6 and chromanone 7. Using two different batchs of Citrus reticulata from two regions of our country Annaba and Skikda, the corresponding optically active alcohols were obtained with high enantioselectivity and Skikda's variety was the best biocatalyst. The results reveal that these plants species can be a promising biocatalysts for the production of key intermediates.
      Graphical abstract image

      PubDate: 2018-02-07T16:27:46Z
      DOI: 10.1016/j.bcab.2018.02.003
       
  • Process optimization for production of a fibrinolytic enzyme from newly
           isolated marine bacterium Pseudomonas aeruginosa KU1
    • Authors: Swaroop S. Kumar; Madhathilkovilakathu Haridas; Abdulhameed Sabu
      Abstract: Publication date: Available online 5 February 2018
      Source:Biocatalysis and Agricultural Biotechnology
      Author(s): Swaroop S. Kumar, Madhathilkovilakathu Haridas, Abdulhameed Sabu
      A potent fibrinolytic enzyme producing bacteria, Pseudomonas aeruginosa KU1 was isolated from marine sediments of Ezhara beach, Kannur, Kerala. Enzyme production was optimized using statistical approach. Placket-Burman factorial design was used in picking key factors (Tryptone, skimmed milk and inoculum size) that influence fibrinolytic enzyme production of the isolate and further optimized using Box-Benhken design. Optimal concentrations for the selected independent variables in the medium were identified to be 0.72% w/v tryptone, 0.09% w/v skimmed milk and 3.95% v/v inoculum size. Peak production was achieved empirically in shake flask culture and it was very close to the projected activity by the response surface model. The Peak production showed 3.25 fold increase over the activity prior to any optimization and a maximum of 1.32 fold increase of one factor at a time optimization. Though many reports are available on fibrinolytic enzyme production from Pseudomonas sp. media optimization studies for enhancement of fibrinolytic enzyme production has not been performed so far. This may be the first report on statistical optimization of production of a fibrinolytic enzyme from marine Pseudomonas sp.
      Graphical abstract image

      PubDate: 2018-02-07T16:27:46Z
      DOI: 10.1016/j.bcab.2018.02.001
       
 
 
JournalTOCs
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Email: journaltocs@hw.ac.uk
Tel: +00 44 (0)131 4513762
Fax: +00 44 (0)131 4513327
 
Home (Search)
Subjects A-Z
Publishers A-Z
Customise
APIs
Your IP address: 54.81.105.205
 
About JournalTOCs
API
Help
News (blog, publications)
JournalTOCs on Twitter   JournalTOCs on Facebook

JournalTOCs © 2009-