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BIOTECHNOLOGY (227 journals)                  1 2 | Last

Showing 1 - 200 of 227 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 7)
Advances in Bioscience and Biotechnology     Open Access   (Followers: 15)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 8)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 69)
American Journal of Bioinformatics Research     Open Access   (Followers: 8)
American Journal of Polymer Science     Open Access   (Followers: 30)
Animal Biotechnology     Hybrid Journal   (Followers: 10)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Bioenergy     Open Access  
Applied Biosafety     Hybrid Journal  
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 62)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 5)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 2)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 1)
Bio-Research     Full-text available via subscription   (Followers: 2)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal  
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 5)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 5)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 6)
Biomédica     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 7)
Biomedical glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Bioprinting     Hybrid Journal  
Bioresource Technology Reports     Hybrid Journal  
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 22)
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 2)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 7)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 5)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 160)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 14)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 1)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 7)
Biotechnology for Biofuels     Open Access   (Followers: 11)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 16)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 39)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 2)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Biotribology     Hybrid Journal  
BMC Biotechnology     Open Access   (Followers: 16)
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Contributions to Tobacco Research     Open Access   (Followers: 3)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 55)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 14)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 9)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access   (Followers: 1)
Entomologia Generalis     Full-text available via subscription  
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 13)
Food Science and Biotechnology     Hybrid Journal   (Followers: 9)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 1)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IIOAB Letters     Open Access  
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 1)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 15)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 2)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 2)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 6)
Journal of Applied Biomedicine     Open Access   (Followers: 3)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of Biosecurity, Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 68)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 10)
Journal of Chitin and Chitosan Science     Full-text available via subscription  
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 7)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 3)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 16)
Journal of Integrative Bioinformatics     Open Access  
Journal of International Biotechnology Law     Hybrid Journal   (Followers: 3)
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 14)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 2)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 6)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 11)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 5)
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microalgae Biotechnology     Open Access   (Followers: 2)
Microbial Biotechnology     Open Access   (Followers: 9)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access  
Molecular Biotechnology     Hybrid Journal   (Followers: 16)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 3)
Nanomaterials and Nanotechnology     Open Access  
Nanomaterials and Tissue Regeneration     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  
Nanotechnology Reviews     Hybrid Journal   (Followers: 5)
Nature Biotechnology     Full-text available via subscription   (Followers: 521)
Network Modeling and Analysis in Health Informatics and Bioinformatics     Hybrid Journal   (Followers: 3)
New Biotechnology     Hybrid Journal   (Followers: 4)
Nigerian Journal of Biotechnology     Open Access  
Nova Biotechnologica et Chimica     Open Access  
NPG Asia Materials     Open Access  
npj Biofilms and Microbiomes     Open Access  
OA Biotechnology     Open Access  
Plant Biotechnology Journal     Open Access   (Followers: 10)
Plant Biotechnology Reports     Hybrid Journal   (Followers: 4)
Preparative Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)

        1 2 | Last

Journal Cover Journal of Applied Biomedicine
  [SJR: 0.277]   [H-I: 18]   [3 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 1214-021X - ISSN (Online) 1214-0287
   Published by Elsevier Homepage  [3177 journals]
  • The influence of modulators of acetylcholinesterase on the resistance of
           mice against soman and on the effectiveness of antidotal treatment of
           soman poisoning in mice

    • Authors: Jiri Kassa; Jan Korabecny; Eugenie Nepovimova; Daniel Jun
      Pages: 10 - 14
      Abstract: Publication date: February 2018
      Source:Journal of Applied Biomedicine, Volume 16, Issue 1
      Author(s): Jiri Kassa, Jan Korabecny, Eugenie Nepovimova, Daniel Jun
      The potency of one reversible inhibitor of acetylcholinesterase (6-chlorotacrine), one reactivator of acetycholinesterase (K027) and their combination to increase the resistance of mice against soman and the efficacy of antidotal treatment of soman-poisoned mice was evaluated. While 6-chlorotacrine was able to markedly protect mice against acute toxicity of soman and the pharmacological pretreatment with 6-chlorotacrine increased the efficacy of antidotal treatment (the oxime HI-6 in combination with atropine) of soman-poisoned mice more than two times, the bispyridinium oxime K027 did not protect mice from acute toxicity of soman, however, the pharmacological pretreatment with this compound was able to markedly increase the efficacy of antidotal treatment of soman-poisoned mice. On the other hand, the combination of both modulators of acetylcholinesterase did not increase the prophylactic efficacy of 6-chlorotacrine alone. These findings demonstrate that pharmacological pretreatment of soman-poisoned mice can be promising and useful in the case of administration of 6-chlorotacrine while the administration of the oxime K027 did not bring any additional benefit when combined with 6-chlorotacrine.
      Graphical abstract image

      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2017.01.004
  • IL-37 mediates the anti-tumor activity in non-small cell lung cancer
           through IL-6/STAT3 pathway

    • Authors: Yan-Ming Deng; Hua Zhang; Jian-Miao Liang; Hai-Bing Xian; Ze-Cheng Chen; Yi-Cong Tang; Shuang Yang; Wei-Neng Feng
      Pages: 15 - 21
      Abstract: Publication date: February 2018
      Source:Journal of Applied Biomedicine, Volume 16, Issue 1
      Author(s): Yan-Ming Deng, Hua Zhang, Jian-Miao Liang, Hai-Bing Xian, Ze-Cheng Chen, Yi-Cong Tang, Shuang Yang, Wei-Neng Feng
      The occurrence and development of lung cancer is closely related to inflammation. Thus, we conducted the present study to investigate the effects of IL-37 (Interleukin 37), a newly identified anti-inflammatory factor, on non-small cell lung cancer (NSCLC), which accounts for about 85% of all lung cancers. To address the function of IL-37 in NSCLC, we first evaluated IL-37 expression in the human NSCLC tissues; then the IL-37 function was assessed in vitro and in vivo in a xenografted lung tumor model. IL-37 was barely expressed in the NSCLC tissue but highly expressed in the adjacent normal tissue. This expression profile was validated by ELISA (Enzyme-linked immunoassay), western blot and immunohistochemical staining. Recombinant IL-37 could suppress cell migration, invasion and proliferation and promote cell apoptosis in NSCLC cell line A549 and SK-MES-1. IL-37 inhibited the IL-6/STAT3 pathway and also the downstream targets Bcl-2, NEDD9 and Cyclin D1. Overexpressing IL-6 or constitutive active STAT3 eliminated the anti-tumor effects of IL-37. Furthermore, IL-37 expression in vivo could inhibit the cancer development. Our results showed that IL-37 plays an inhibitory role in lung cancer development, possibly through IL-6/STAT3 pathway.
      Graphical abstract image

      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2017.04.001
  • Hits and defeats of genome-wide association studies of atopy and asthma

    • Authors: Hanna Danielewicz
      Pages: 161 - 168
      Abstract: Publication date: July 2017
      Source:Journal of Applied Biomedicine, Volume 15, Issue 3
      Author(s): Hanna Danielewicz
      Atopy and asthma are complex conditions, recognised as outcomes in which both genes and environment play crucial role. Large number of disease associated loci have been identified within GWAS approach over last years and the knowledge of pathobiology of asthma and allergy has widened substantially. However still the results achieved are difficult to interpret. Most markers have no clear function on and expound small portion of heritability. The “missed heritability” could be hidden in the gene-by environment interactions. The most know environmental factor which interacts with asthma and atopy is farming. The common link between genes and environment could be epigenetics.
      Graphical abstract image

      PubDate: 2018-01-04T01:28:15Z
      DOI: 10.1016/j.jab.2017.03.002
  • The role of keratinocytes in inflammation

    • Authors: Jana Juráňová; Jana Franková; Jitka Ulrichová
      Pages: 169 - 179
      Abstract: Publication date: July 2017
      Source:Journal of Applied Biomedicine, Volume 15, Issue 3
      Author(s): Jana Juráňová, Jana Franková, Jitka Ulrichová
      The epidermis is the external layer of the skin and is composed mainly of keratinocytes. Therefore, keratinocytes play an indispensable role as inherent constituents of the skin barrier in physical defenses against environmental threats. Keratinocytes also exert an active protective role against invasion by pathogens. This competency is of particular importance when physical defenses fail as a consequence of skin injury. During the inflammatory phase of healing, keratinocytes act as immuno-modulators, managing inflammation via a rigorously coordinated network of inflammatory cascades, triggered by keratinocyte-receptor communication with the surroundings in a paracrine and autocrine manner. This review summarizes current understandings of the coordinated inflammatory network and focuses on recent progress regarding the role of keratinocytes in early phases of skin wound healing.
      Graphical abstract image

      PubDate: 2018-01-04T01:28:15Z
      DOI: 10.1016/j.jab.2017.05.003
  • Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles
           with antimicrobial, antioxidant and cytotoxic activities

    • Authors: Annu; Shakeel Ahmed; Gurpreet Kaur; Praveen Sharma; Sandeep Singh; Saiqa Ikram
      Abstract: Publication date: Available online 12 March 2018
      Source:Journal of Applied Biomedicine
      Author(s): Annu, Shakeel Ahmed, Gurpreet Kaur, Praveen Sharma, Sandeep Singh, Saiqa Ikram
      Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV–vis spectroscopy, Dynamic light scattering, and High Resolution Transmission Electron Microscopy. TEM studies revealed 9–46 nm size range of synthesized AgNPs. The antimicrobial and antioxidant activities were also studied by using Agar well diffusion method and DPPH Assay, respectively. Nanoparticles showed good antimicrobial activity against both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. Further, bioactivity assays revealed selective cytotoxicity (anticancer) of the nanoparticles against human lung cancer cell line A549. The nanoparticles are able to induce cancer cell specific apoptosis at G0/G1 phase of cell cycle. The results showed potential mechanism of action of nanoparticles via augmentation of antioxidant system in cancer cells. Over all, this study show multifaceted potential bioactivities of nanoparticles generated from fruit waste.
      Graphical abstract image

      PubDate: 2018-03-18T20:37:07Z
      DOI: 10.1016/j.jab.2018.02.002
  • Naringenin alleviates high d-glucose-induced injuries through upregulation
           of microRNA-30d-5p level in human AC16 cardiac cells

    • Authors: Jiamei Jiang; Guobiao Liang; Zijun Wu; Hailiang Mo; Qiong You; Zhiqiang Wang; Keng Wu; Runmin Guo
      Abstract: Publication date: Available online 2 March 2018
      Source:Journal of Applied Biomedicine
      Author(s): Jiamei Jiang, Guobiao Liang, Zijun Wu, Hailiang Mo, Qiong You, Zhiqiang Wang, Keng Wu, Runmin Guo
      As a common complication of diabetes mellitus (DM), diabetic cardiomyopathy (DCM) is considered to be one of the major causes of mortality and morbidity. The therapeutic effects of naringenin have been verified in the treatment of various human diseases. However, the application of naringenin in the treatment of DCM still has not been reported. In this study, human AC16 cardiac cells were treated with normal d-glucose and high d-glucose (HG). After transfection with miR-30d-5p inhibitor, Cell Counting Kit-8 (CCK-8) method was used to measure cell viability. Hoechst 33258 staining was performed to observe the morphological changes of nucleus. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the activity of caspase-3. Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. Levels of light chain 3 (LC3) including LC3-I and LC3-II as well as nucleoporin p62 (P62) were detected by Western blot. We found that Naringenin treatment increased the reduced cell variability caused by HG treatment. Naringenin also increased expression level of miR-30d-5p in human AC16 cardiac cells after HG treatment. Treatment with miR-30d-5p inhibitor reduced the effect of miR-30d-5p in increasing cell variability and reducing cell apoptosis. Naringenin treatment reduced the increased levels of LC-I, LC-II and P62, but miR-30d-5p inhibitor reduced those changes. Therefore we concluded that naringenin could alleviate HG-induced injuries through the upregulation of microRNA-30d-5p level in human AC16 cardiac cells.

      PubDate: 2018-03-06T17:24:51Z
      DOI: 10.1016/j.jab.2018.02.005
  • Genetic association of Tumour necrosis factor alpha, Interleukin-18 and
           Interleukin 1 beta with the risk of coronary artery disease: A
           case-control study outcome from Kashmir

    • Authors: Nuzhat Shaheen Khan; Mohammad Sultan Allai; Bushra Nissar; Niyaz Ahmad Naykoo; Iqra Hameed; Misbah Majid; Aaliya Bhat; Falaque ul Afshan; Bashir Ahmad Ganai
      Abstract: Publication date: Available online 27 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Nuzhat Shaheen Khan, Mohammad Sultan Allai, Bushra Nissar, Niyaz Ahmad Naykoo, Iqra Hameed, Misbah Majid, Aaliya Bhat, Falaque ul Afshan, Bashir Ahmad Ganai
      Coronary artery disease (CAD) is a clinical manifestation of atherosclerosis in the arteries supplying myocardium. Inflammation is the cornerstone in the development and progression of atherosclerosis. Amongst the various biomolecules tumour necrosis factor-α (TNF-α), interleukin-18 (IL-18) and interleukin-1β (IL-1β) build an inflammatory bionetwork in developing the disease. In this study we investigated the association of TNF-α SNPs [–308G/A (rs1800629), −1031T/C (rs1799964), −863C/A (rs1800630)]; IL-18 [–137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), −31C/T (rs1143627), and −511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the −308G/A-A/A and −863A/A showed an association with disease while −1031T/C was found to have an inverse relation. The IL-18–137G/C showed no statistically significant difference between controls and cases. For IL-1β the +3954C/T and −31C/T SNP variants showed no disease association while −511T/T showed significant association. Haplotypic analysis revealed the haplotype ATCGCC and GTACCTC to be associated with CAD risk and GTCGTTT, in particular, showing a profound association. Overall, our study suggests that TNF-α and IL-1β promoter polymorphisms may act as genetic risk factors in developing the coronary artery disease.
      Graphical abstract image

      PubDate: 2018-03-06T17:24:51Z
      DOI: 10.1016/j.jab.2018.02.004
  • RIG-1 and MDA5 are the important intracellular sensors against bacteria in
           septicemia suffering patients

    • Authors: Asieh Asadpour-Behzadi; Ashraf Kariminik
      Abstract: Publication date: Available online 21 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Asieh Asadpour-Behzadi, Ashraf Kariminik
      Background MDA5 and RIG-1 are the important intracellular receptors which detect microbial associated molecular patterns. Septicemia is a condition in which infection enters the bloodstream of the patients. The main intracellular mechanisms against septicemia are yet to be clarified. Therefore, this research study was aimed to evaluate expression of MDA5 and RIG-1 in the patients suffering from septicemia in comparison to healthy controls. Methods MDA5 and RIG-1 expression levels in 40 patients suffering from septicemia and 40 healthy controls were evaluated using Real-Time PCR technique. The sources of bacteria in the bloodstream of the patients suffering from septicemia were determined using microbial cultures. Results The results showed that mRNA levels of MDA5 and RIG-1 were significantly increased in the patients when compared to healthy controls. The results also revealed that the patients were infected with four bacteria including Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii and Pseudomonas aeruginosa. mRNA levels of MDA5 and RIG-1 did not differ among patients with various bacterial infections. Conclusion Based on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity.
      Graphical abstract image

      PubDate: 2018-02-26T15:13:33Z
      DOI: 10.1016/j.jab.2018.01.009
  • Lectin-based analysis of human milk immunoglobulin G fucosylated variants
           in relation to milk maturation and perinatal risk factors

    • Authors: Jolanta Lis-Kuberka; Magdalena Orczyk-Pawiłowicz; Barbara Królak-Olejnik; Marta Berghausen-Mazur; Karolina Barańska; Iwona Kątnik-Prastowska
      Abstract: Publication date: Available online 13 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Jolanta Lis-Kuberka, Magdalena Orczyk-Pawiłowicz, Barbara Królak-Olejnik, Marta Berghausen-Mazur, Karolina Barańska, Iwona Kątnik-Prastowska
      Background Fucosylated glycotopes of milk immunoglobulin G (IgG) are ligands in reactions of biological recognition protecting newborns against infection and ensuring proper development. Materials and methods Relative amounts of IgG fucosyl-glycovariants in milk of mothers giving birth to term and premature newborns (term and preterm milk groups) were analysed by lectin-IgG-ELISA using α1,2-, α1,3-, and α1,6-fucose specific biotinylated Ulex europaeus (UEA), Tetragonolobus purpureus (LTA), and Lens culinaris (LCA) lectins, respectively. Results The term and preterm milk IgG glycovariants were highly reactive with UEA, LTA, and LCA, whereas maternal plasma IgG poorly or at all. During milk maturation the IgG of very preterm and preterm milk compared to term milk differed by lower relative amounts of UEA-, higher of LTA-, and nearly stable expression of LCA-reactive glycotopes. Moreover, lower α1,2- and higher α1,3- relative amounts of lectin-dependent milk IgG-fucosylated glycovariants were found to be associated with an infectious disease of lactating mothers. Conclusion The highly fucosylated glycovariants of human IgG given with mothers’ milk to immunologically immature newborn seem to be bifunctional molecules with potential therapeutic properties. The analysis of fucosylation status of milk IgG by simple lectin-IgG-ELISA may be helpful to control the immunological quality of milk for milk banking.
      Graphical abstract image

      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.02.001
  • JNK inhibitor CC-930 reduces fibrosis in a murine model of Nf1-deficient
           fracture repair

    • Authors: Nikita Deo; Jad El-Hoss; Mille Kolind; Kathy Mikulec; Lauren Peacock; David G. Little; Aaron Schindeler
      Abstract: Publication date: Available online 13 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Nikita Deo, Jad El-Hoss, Mille Kolind, Kathy Mikulec, Lauren Peacock, David G. Little, Aaron Schindeler
      Tibial pseudarthrosis often features deficient bone formation, excessive bone resorption, and extensive pathological fibrosis, particularly in individuals with Neurofibromatosis type I (NF1). It was hypothesized that overactive NF1-Ras-JNK signalling may underlie the pathological fibrosis, and that this could be treated via a JNK antagonist. CC-930, a small molecule JNK inhibitor, was trialed in closed fractures in wild type mice CC-930 (25 mg/kg/twice daily) was dosed throughout fracture healing (D2–21) and during the latter stages of repair (D11–21). All fractures healed by D21, regardless of treatment, with some of the CC-930 (D11–21) treatment group showing early bridging. CC-930 (D11–21) was tested in an Nf1-null fracture model where Nf1 was inactivated by Ad-Cre virus injection in Nf1flox/flox mice; these mice also possessed a Cre-responsive tdTomato transgene. CC-930 resulted in a significant decrease in non-unions (93% vehicle vs. 64% CC-930, p < 0.01). Local treatment with the bone anabolic rhBMP-2 (10 μg) increased union and callus bone volume, but also increased the fibrotic tissue at the fracture site. Fractures treated with a combination of rhBMP-2 (10 μg) and CC-930 were all partially or fully bridged by D21 (p < 0.01 vs. vehicle) and there was a significant decrease in fibrosis vs. rhBMP-2 alone (p < 0.01). In untreated Nf1-null fractures, the tdTomato transgene was expressed in fibrous tissue at the fracture site, but not in the newly forming bone. These data suggest that JNK inhibition may be an effective therapeutic approach for reducing pathological fibrosis in NF1 tibial pseudarthrosis, however other adjunctive strategies may be required to augment bone formation.

      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.006
  • Antioxidant and anti-inflammatory mechanisms of polyphenols isolated from
           virgin coconut oil attenuate cadmium-induced oxidative stress-mediated
           nephrotoxicity and inflammation in rats

    • Authors: Ademola C. Famurewa; Abumchukwu J. Ejezie; Chioma S. Ugwu-Ejezie; Ebele J. Ikekpeazu; Fidelis E. Ejezie
      Abstract: Publication date: Available online 10 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Ademola C. Famurewa, Abumchukwu J. Ejezie, Chioma S. Ugwu-Ejezie, Ebele J. Ikekpeazu, Fidelis E. Ejezie
      Purpose Cadmium (Cd) is a classic cumulative nephrotoxicant and literature suggests that its toxicity is associated with oxidative stress and inflammation which contribute to pathologies in various tissues. We sought to investigate whether polyphenols isolated from virgin coconut oil (VCO) would modulate nephrotoxicity and inflammation induced by Cd in rats. Methods Rats were administered polyphenols prior to and along with Cd (5 mg/kg, orally) for 7 weeks. Serum markers of renal damage, interleukin-6 (IL-6), C-reactive protein (CRP) and nitric oxide (NO) were evaluated; renal activities of antioxidant enzymes, as well as malondialdehyde (MDA) and reduced glutathione (GSH) content were determined. Histopathologic alterations were evaluated to define kidney damage. Results Cadmium exposure induced nephrotoxicity and oxidative stress evident by significantly increased serum levels of creatinine, urea, and uric acid along with remarkable depression in renal activities of antioxidant enzymes and GSH with prominent increase in MDA. Inflammatory markers – IL-6, CRP and NO were significantly increased and confirmed by histopathology. Sub-chronic administration of VCO polyphenols attenuated the Cd-induced biochemical alterations compared to Cd control with remarkably improved histopathological observations. Conclusion The findings showed that VCO polyphenol supplementation protects against Cd-induced nephrotoxicity via its antioxidant and anti-inflammatory mechanisms in rats.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.02.003
  • Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and
           sulfate metabolism of Desulfovibrio piger

    • Authors: Ivan Kushkevych; Monika Vítězová; Jiří Kos; Peter Kollár; Josef Jampílek
      Abstract: Publication date: Available online 10 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Ivan Kushkevych, Monika Vítězová, Jiří Kos, Peter Kollár, Josef Jampílek
      An increased number of sulfate-reducing bacteria is often isolated from faeces of patients with gastrointestinal diseases, which can be the cause of the development of bowel inflammation. Frequent use of antibiotics causes the resistance of intestinal microorganisms and ineffective treatment of these diseases. The antimicrobial activity and biological properties of the selected ring-substituted 8-hydroxyquinoline-2-carboxanilides against Desulfovibrio piger Vib-7 were studied. The addition of these compounds in the cultivation medium inhibited the bacterial growth and the process of sulfate reduction dose-dependently. A significant cytotoxic activity under the influence of ring-substituted 8-hydroxyquinoline-2-carboxanilides was determined. The strongest cytotoxic effect of the derivatives was observed for compounds 8-hydroxy-N-(3-methoxyphenyl)quinoline-2-carboxamide and 8-hydroxy-N-(3-trifluoromethylphenyl)quinoline-2-carboxamide that caused a low survival of D. piger Vib-7 in concentration 17 μM and high toxicity rates.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.004
  • Induction of caspase-mediated apoptosis using Alnus japonica extracts in
           AGS human gastric carcinoma cells

    • Authors: Seong-Eun Kim; Yon-Suk Kim; Woen-Bin Shin; Jin-Su Park; Sang-Ho Moon; Byong-Tae Jeon; Pyo-Jam Park
      Abstract: Publication date: Available online 9 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Seong-Eun Kim, Yon-Suk Kim, Woen-Bin Shin, Jin-Su Park, Sang-Ho Moon, Byong-Tae Jeon, Pyo-Jam Park
      Alnus japonica has been used as a traditional oriental medicine for many diseases such as fever, haemorrhage and alcoholism. In this study, A. japonica extracts were evaluated for their in vitro antioxidant potentials and anticancer effects in AGS human gastric carcinoma cell line. The antioxidant properties of A. japonica extracts were evaluated using several biochemical assays, including FRAP (ferric reducing antioxidant power) assay, ABTS (2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), alkyl and hydroxyl radical scavenging activity assay. Our study showed that ethanol extract of A. japonica (AJE) has a more potent antioxidant activity than its water extract. In addition, AJE extract inhibited the cell growth and induced the cell death by increasing reactive oxygen species (ROS) production in AGS cells. Moreover, AJE extract specifically triggered the apoptosis mediated through the activation of caspase-8, 7, 3, and poly-ADP ribose polymerase (PARP). Thus, these results suggest that AJE extract could be potentially useful as a new promising strategy in the therapy for gastric carcinoma cancer.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.008
  • Cell-free nucleic acids in urine as potential biomarkers of kidney disease

    • Authors: Marianna Gyurászová; Alexandra Kovalčíková; Janka Bábíčková; Július Hodosy; Ľubomíra Tóthová
      Abstract: Publication date: Available online 9 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Marianna Gyurászová, Alexandra Kovalčíková, Janka Bábíčková, Július Hodosy, Ľubomíra Tóthová
      Kidney and uropoetic system diseases represent a major social, economic and health burden. This is mainly because early diagnosis of kidney dysfunction is currently unavailable, since the current markers are often reliably increased only after advanced progression of the renal diseases. Recently, circulating nucleosomes, DNA and numerous forms of RNA have been detected in human biological fluids, such as plasma, urine, saliva, and breast milk. Although their biological functions remain mostly unknown, they are attractive as potential biomarkers of various diseases. In urine, many of the circulating nucleic acids originate from the cells of the kidney and the urinary tract making these non-invasive and easily obtained new biomarkers in the nephrology or urology. This review focuses on cell free nucleic acids in urine and its potential in human studies. Although, there are some technical and biological limitations, the urinary circulating nucleic acids hold a great potential as new biomarkers of renal diseases.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.007
  • Quantification of uPA in breast tumour tissue extracts by microarray
           immunoassay: Comparison with ELISA technology

    • Authors: Liu Shi; Thomas Gehin; Yann Chevolot; William Jacot; Pierre-Jean Lamy; Emmanuelle Laurenceau
      Abstract: Publication date: Available online 3 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Liu Shi, Thomas Gehin, Yann Chevolot, William Jacot, Pierre-Jean Lamy, Emmanuelle Laurenceau
      The urokinase-type plasminogen activator (uPA) and PA inhibitor 1 (PAI-1) play important roles in breast cancer metastasis through cell migration and invasion. They are clinically applicable prognostic and predictive markers. High levels of uPA and PAI-1 are associated with high risk of recurrence and adjuvant chemotherapy provides substantial benefit for this breast cancer population. The current sole validated method for quantifying uPA level in breast tumour tissue is ELISA assay. It requires 50–300 mg of fresh or frozen tissue, which is the main limitation for routine use. In this study, we evaluated the performances of customized antibody microarray to quantify uPA concentration from reduced extraction solution of breast tumour tissue and compared it with standard ELISA kit. We firstly optimized the elaboration of customized antibody microarray in order to sensitively detect and quantify uPA standard solutions. In the best conditions, we analysed uPA concentration in 16 cytosolic extracts from breast tumour tissue. Results showed that our customized antibody microarray could correctly quantify uPA concentration while consuming 100 times less volume of tumour tissue extraction solution than ELISA. Our antibody microarray is a powerful and promising tool for the miniaturization of the immunoassay quantification of uPA from breast tumour tissue extracts.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.001
  • Effect of gender, age, diet and smoking status on the circadian rhythm of
           ascorbic acid (vitamin C) of healthy Indians

    • Authors: Ranjana Singh; Abbas Ali Mahdi; Raj Kumar Singh; Cathy Lee Gierke; Germaine Cornelissen
      Abstract: Publication date: Available online 1 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): Ranjana Singh, Abbas Ali Mahdi, Raj Kumar Singh, Cathy Lee Gierke, Germaine Cornelissen
      Background/Objectives To determine effects of gender, age, diet, and smoking status on circadian rhythm characteristics of ascorbic acid (vitamin C). Subjects/Methods Ascorbic acid was measured spectrophotometrically in serum collected from 162 healthy volunteers (103 males and 59 females; 7–75 years) every 6 h for 24 h (4 samples). Data were analyzed by single and population mean cosinor. Effects of gender, age, diet (vegetarian vs. omnivore), and smoking status on the rhythm-adjusted mean (MESOR) and circadian amplitude were examined by multiple analysis of variance. Results A circadian rhythm is documented with statistical significance by population mean cosinor. In addition to effects of gender and age, the MESOR is affected by diet and smoking status. The circadian amplitude changes nonlinearly as a function of age. The circadian acrophase advances with increasing age. Conclusion The present observations confirm a definite circadian rhythm in ascorbic acid concentrations with significant effects of age, diet and smoking status in clinical health. Mapping the circadian rhythm of serum ascorbic acid in health can help explore its role in different pathophysiological conditions as pre-disease conditions may be characterized by alterations in the circadian amplitude and/or phase before there is a change in mean value.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.003
  • Oral melatonin administration improves the objective and subjective sleep
           quality, increases 6-sulfatoxymelatonin levels and total antioxidant
           capacity in patients with fibromyalgia

    • Authors: M. Yolanda Castaño; Maria Garrido; Jonathan Delgado-Adámez; Sara Martillanes; M. Ángeles Gómez; Ana Beatriz Rodríguez
      Abstract: Publication date: Available online 1 February 2018
      Source:Journal of Applied Biomedicine
      Author(s): M. Yolanda Castaño, Maria Garrido, Jonathan Delgado-Adámez, Sara Martillanes, M. Ángeles Gómez, Ana Beatriz Rodríguez
      Background/objective Chronic pain, sleep disturbances and oxidative stress are implicated in the pathogenesis of fibromyalgia. The aim of this study was to assess the effect of melatonin supplementations on sleep quality, 6-sulfatoxymelatonin (aMT6-s) levels, as well as urinary and serum total antioxidant capacity (TAC) in patients with fibromyalgia. Methods Thirty three patients carried out the full study. A baseline period (10 days) was included in the experimental design to collect information about patients’ initial status. Then, patients took different doses of melatonin, during 10 consecutive days each. Placebo was given during 10 days either before or between melatonin doses. Objective sleep quality was recorded by actigraphy whereas subjective sleep quality was measured by The Pittsburgh Sleep Quality Index. Quantification of aMT6-s and TAC was achieved by ELISA and colorimetric assay kits, respectively. Results Six out of seven sleep parameters evaluated by actigraphy were improved after the intake of melatonin as well as the subjective sleep quality. All the biochemical parameters measured were also elevated after the melatonin administration. Conclusion The daily intake of melatonin improved the sleep quality, increased the aMT6-s levels and the total antioxidant capacity in patients with fibromyalgia.

      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.005
  • A survey on applying machine learning techniques for management of

    • Authors: Enas M.F. El Houby
      Abstract: Publication date: Available online 19 January 2018
      Source:Journal of Applied Biomedicine
      Author(s): Enas M.F. El Houby
      During the past years, the increase in scientific knowledge and the massive data production have caused an exponential growth in databases and repositories. Biomedical domain represents one of the rich data domains. An extensive amount of biomedical data is currently available, ranging from details of clinical symptoms to various types of biochemical data and outputs of imaging devices. Manually extracting biomedical patterns from data and transforming them into machine-understandable knowledge is a difficult task because biomedical domain comprises huge, dynamic, and complicated knowledge. Data mining is capable of improving the quality of extracting biomedical patterns. In this research, an overview of the applications of data mining on the management of diseases is presented. The main focus is to investigate machine learning techniques (MLT) which are widely used to predict, prognose and treat important frequent diseases such as cancers, hepatitis and heart diseases. The techniques namely Artificial Neural Network, K-Nearest Neighbour, Decision Tree, and Associative Classification are illustrated and analyzed. This survey provides a general analysis of the current status of management of diseases using MLT. The achieved accuracy of the various applications ranged from 70% to 100% according to the disease, the solved problem, and the used data and technique.
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      PubDate: 2018-02-16T08:35:26Z
      DOI: 10.1016/j.jab.2018.01.002
  • NTPDase, 5′-nucleotidase and adenosine deaminase activities and purine
           levels in serum of sickle cell anemia patients

    • Authors: Lívia G. Castilhos; Stephen A. Adefegha; Pedro H. Doleski; Tatiana M.D. Bertoldo; Cesar Eduardo J. Moritz; Emerson A. Casali; Daniela B.R. Leal
      Abstract: Publication date: Available online 26 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Lívia G. Castilhos, Stephen A. Adefegha, Pedro H. Doleski, Tatiana M.D. Bertoldo, Cesar Eduardo J. Moritz, Emerson A. Casali, Daniela B.R. Leal
      Sickle cell anemia (SCA) is a hereditary disorder that is characterized by tendency of hemoglobin molecules within the erythrocytes to polymerize under hypoxia conditions, deform the red cells, and promote vaso-occlusion and endothelial damage. This disease may be associated with the extracellular release of nucleotides, particularly ATP, ADP and adenosine into the circulation. The aim of this study was to investigate the possible changes in adenine nucleotides and nucleoside metabolizing enzymes as well as their levels in serum of SCA patients. NTPDase, 5′-nucleotidase and adenosine deaminase activities were evaluated in serum obtained from blood samples of 15 SCA treated patients and 15 healthy subjects (control group). The results revealed that there were significant (P < 0.001) elevations in NTPDase, 5′-nucleotidase and ADA activities in serum of SCA treated patients when compared to the control group. Furthermore, no significant (P > 0.05) alteration was observed in ATP, ADP and adenosine levels of both SCA treated and control groups. However, inosine level was significantly (P < 0.05) decreased and hypoxanthine level was higher in SCA treated patients (P < 0.001) when compared to the control group. The results suggest the involvement of purinergic signaling enzymes in the maintenance of the levels of extracellular nucleotides and nucleosides, thus preventing some pathophysiological conditions associated with SCA.
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      PubDate: 2018-01-04T01:28:15Z
      DOI: 10.1016/j.jab.2017.12.004
  • Influence of skin permeation enhancers on the transdermal delivery of
           palonosetron: An in vitro evaluation

    • Authors: Anroop B. Nair; Shery Jacob; Bandar E. Al-Dhubiab; Rakan Naser Alhumam
      Abstract: Publication date: Available online 21 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Anroop B. Nair, Shery Jacob, Bandar E. Al-Dhubiab, Rakan Naser Alhumam
      It is proposed that the low skin permeation potential of palonosetron could be enhanced by the inclusion of chemical permeation enhancers. The objective of this study is to evaluate the influence of various chemical enhancers on the transdermal permeation of palonosetron. Different drugs in adhesive transdermal patches (F1–F5) were prepared using five pressure sensitive adhesives; Duro-Tak 87-4098, Duro-Tak 87-2074, Duro-Tak 87-900A, Duro-Tak 87-9301 and Duro-Tak 87-2287. Patches prepared using Duro-Tak 87-9301 (F5) was further combined with four well-known chemical enhancers. The influence of permeation enhancers (propylene glycol, diethylene glycol monoethyl ether, Tween 80 and oleic acid) on the transdermal flux was evaluated ex vivo. Release of the drug from fabricated patches was carried out for a period of 6 h. Greater amount of drug (12% w/w) was incorporated in the patches prepared using Duro-Tak 87-9301 (F5). Incorporation of skin permeation enhancers significantly (P < 0.001) improves the transdermal flux of palonosetron. Among the permeation enhancers, propylene glycol (5% w/w) shows highest permeation (53.12 ± 5.62 μg/cm2/h), which is ∼4 folds higher than control. Biphasic drug release was noticed in the prepared patches and the rate of release was relatively high with patch F7. This study reveals that the optimized transdermal system with propylene glycol as permeation enhancers can provide effective therapeutic level of palonosetron.
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      PubDate: 2017-12-26T22:40:43Z
      DOI: 10.1016/j.jab.2017.12.003
  • Nonlinear Heart Rate Variability based artificial intelligence in lung
           cancer prediction

    • Authors: Reema Shyamsunder Shukla; Yogender Aggarwal
      Abstract: Publication date: Available online 21 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Reema Shyamsunder Shukla, Yogender Aggarwal

      PubDate: 2017-12-26T22:40:43Z
      DOI: 10.1016/j.jab.2017.12.002
  • In vivo assessment of time dependent changes of T2* in medial meniscus
           under loading at 3T: A preliminary study

    • Authors: Lenka Hornakova; Vladimir Juras; Petr Kubovy; Daniel Hadraba; David Gerych; Pavel Stursa; Xeni Deligianni; Oliver Bieri; Siegfried Trattnig; Karel Jelen
      Abstract: Publication date: Available online 15 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Lenka Hornakova, Vladimir Juras, Petr Kubovy, Daniel Hadraba, David Gerych, Pavel Stursa, Xeni Deligianni, Oliver Bieri, Siegfried Trattnig, Karel Jelen
      Due to the internal structure of the knee joint, the ability to characterize and quantify the dynamic response of the meniscal tissue directly in vivo is highly problematic. The main purpose of this study was to investigate the behaviour of the meniscus under loading conditions. Four healthy young females were included. To obtain T2* values in the meniscus, the vTE sequence was used with 10 echoes ranging from 0.8 to 10.1 ms. Submilisecond first echo time is a great advantage of vTE sequence allowing for precise mapping of relatively short T2*. The two-parametric least squares fitting procedure was used to calculate T2* pixel-wise. A custom-made diamagnetic apparatus was developed to simulate stress conditions on the lower limb in a conventional MR scanner. vTE T2* was performed in five consecutive scans, 6:10 min apart. Three different compartments of the medial and lateral meniscus were segmented. The differences at the different time-points were calculated. A constant increase of T2* times after compression was statistically significant in the anterior horn of the medial meniscus. T2* mapping with variable echo time sequence might be a satisfactorily sensitive technique to detect the changes of meniscus physiology under loading conditions in vivo.
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      PubDate: 2017-12-19T19:09:53Z
      DOI: 10.1016/j.jab.2017.12.001
  • Caveolin-1 rs4730751 gene polymorphism in kidney allograft recipients

    • Authors: Sylwia Słuczanowska-Głąbowska; Damian Malinowski; Krzysztof Safranow; Leszek Domański; Michał Czerewaty; Przemysław Ustianowski; Maria Laszczyńska; Andrzej Pawlik
      Abstract: Publication date: Available online 6 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Sylwia Słuczanowska-Głąbowska, Damian Malinowski, Krzysztof Safranow, Leszek Domański, Michał Czerewaty, Przemysław Ustianowski, Maria Laszczyńska, Andrzej Pawlik
      Caveolin-1 is a phosphoprotein that plays a crucial role in tissue remodeling and fibrotic pathways, controlling fibroblast function, proliferation, and apoptosis. Here we aimed to examine the association between the CAV1 rs4730751 gene polymorphism and kidney graft function, graft histology, and graft survival. This study enrolled 270 Caucasian deceased – donor renal transplant recipients. We found no statistically significant associations between CAV1 rs4730751 and delayed graft function, acute rejection, or chronic allograft dysfunction, as well as creatinine values at 1–60 months after transplantation, the risk of graft loss, dialysis or death after transplantation, or histopathological changes in kidney allograft biopsies. Our findings do not support an association between the CAV1 gene rs4730751 polymorphism and kidney allograft function in our population.
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      PubDate: 2017-12-19T19:09:53Z
      DOI: 10.1016/j.jab.2017.11.007
  • Effect of pramipexole alginate nanodispersion (PAND) on the transgenic
           Drosophila expressing human alpha synuclein in the brain

    • Authors: Yasir Hasan Siddique; Falaq Naz; Wasi Khan; Smita Jyoti; Braj Raj Singh; A.H. Naqvi
      Abstract: Publication date: Available online 1 December 2017
      Source:Journal of Applied Biomedicine
      Author(s): Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, A.H. Naqvi
      In the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress markers and dopamine content was also observed in the PD flies exposed to various doses PAND. The improvement in the PD symptoms was more in PD flies exposed to PAND compared to PD flies exposed to pramipexole alone.
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      PubDate: 2017-12-19T19:09:53Z
      DOI: 10.1016/j.jab.2017.11.002
  • Sinomenine inhibits the growth of glioma cells through STAT3 signal

    • Authors: Yi-ting Wei; Qun-ying Yang; Shuang-bo Fan; Liang-Wang; Dong-Xia Hu; Ping Shuai
      Abstract: Publication date: Available online 28 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Yi-ting Wei, Qun-ying Yang, Shuang-bo Fan, Liang-Wang, Dong-Xia Hu, Ping Shuai
      The present study was designed to examine effects of Sinomenine (SM) on glioma cells growth in vivo and in vitro. Cells growth and apoptosis were detected by MTT assay, TUNEL assay and flow cytometric analysis. In the study, SM treatment led to growth inhibition on a series of glioma cell lines, including U87, U373, U251, Hs683 and T98G. SM prevented U87 growth in the nude mice as well. Inhibitory effects of SM on U87 cells proliferation in vitro and in vivo were more effective than that of temozolomide (TMZ), and SM has synergistic effects with TMZ in the glioma therapy. SM induced apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Moreover, we observed SM decreased the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. Interestingly, using a specific activator of STAT3, we demonstrated overexpression of p-STAT3 impaired, SM mediated growth inhibition and apoptosis induction in the U87 cells. In summary, our results indicate SM induced growth suppression of human glioma cells through inhibiting phosphorylation of STAT3.
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      PubDate: 2017-12-19T19:09:53Z
      DOI: 10.1016/j.jab.2017.11.005
  • Proliferation of Toxoplasma gondii (RH strain) is inhibited by the
           combination of pravastatin and simvastatin with low concentrations of
           conventional drugs used in toxoplasmosis

    • Authors: Raquel Arruda Sanfelice; Larissa Rodrigues Bosqui; Suelen Santos da Silva; Milena Menegazzo Miranda-Sapla; Luciano Aparecido Panagio; Italmar Teodorico Navarro; Ivete Conchon-Costa; Wander Rogério Pavanelli; Ricardo Sergio Almeida; Idessania Nazareth Costa
      Abstract: Publication date: Available online 27 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Raquel Arruda Sanfelice, Larissa Rodrigues Bosqui, Suelen Santos da Silva, Milena Menegazzo Miranda-Sapla, Luciano Aparecido Panagio, Italmar Teodorico Navarro, Ivete Conchon-Costa, Wander Rogério Pavanelli, Ricardo Sergio Almeida, Idessania Nazareth Costa
      Toxoplasma gondii, an etiologic agent of toxoplasmosis, is an obligate intracellular parasite, which exhibits an apicoplast organelle which assists in the metabolism of isoprenoids and other pivotal mediators for the parasite survival. Statins are drugs that inhibit cholesterol synthesis, blocking the conversion of the substrate HMG-CoA to mevalonate, thus preventing the initial processes of the biosynthesis of these precursors, both in humans and parasite. In the light of this information, we determined the effect of pravastatin and simvastatin associated with the current drugs (pyrimethamine and sulfadiazine) as a possible alternative treatment for this infection. Cytotoxicity was evaluated in HeLa cells by MTT assay, which was observed the drug combinations did not affect cell viability. HeLa cells (105) were infected with T. gondii tachyzoites of RH strain (5×105) and treated with pravastatin and/or simvastatin combined with pyrimethamine and/or sulfadiazine for 24h. Our data showed a significant reduction in cell adhesion, infection and mainly parasite proliferation in all treatments. Based on these results, the combination of statins with drugs used in current therapy showed to be a promising therapeutic alternative for toxoplasmosis treatment.
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      PubDate: 2017-11-28T08:56:01Z
      DOI: 10.1016/j.jab.2017.10.009
  • DNA repair inhibitors as radiosensitizers in human lung cells

    • Authors: Kamila Ďurišová; Lucie Čecháková; Petr Jošt; Zuzana Šinkorová; Adéla Kmochová; Jaroslav Pejchal; Martin Ondrej; Jiřina Vávrová; Aleš Tichý
      Abstract: Publication date: Available online 27 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Kamila Ďurišová, Lucie Čecháková, Petr Jošt, Zuzana Šinkorová, Adéla Kmochová, Jaroslav Pejchal, Martin Ondrej, Jiřina Vávrová, Aleš Tichý
      The aim of this study was to compare the effects of DNA repair inhibitors in the context of radio-sensitization of human lung cells. The radio-sensitizing effects of NU7441 (1 mM), an inhibitor of DNA-dependent protein kinase (DNA-PK); KU55933 (10 μM), an inhibitor of ataxia-telangiectasia mutated kinase (ATM); and VE-821 (10 μM), an inhibitor of ATM-related kinase (ATR) were tested by the xCELLigence system for monitoring proliferation, fluorescence microscopy for DNA damage detection, flow-cytometry for cell cycle and apoptosis analysis and western blotting and ELISA for determination of DNA repair proteins. We employed normal human lung fibroblasts (NHLF, p53-wild-type) and non-small cell lung cancer cells (H1299, p53-negative). DNA-PK inhibition (by NU7441) in combination with ionizing radiation (IR) increased the number of double strand breaks (DSB), which persisted 72h after irradiation in both cell lines. Additionally, NU7441 and KU55933 in combination with IR caused G2-arrest. ATR inhibitor (VE-821) together with IR markedly inhibited proliferation and induced G2/M arrest accompanied by apoptosis in H1299, but not in NHLF cells, and thus diminished DNA-repair of tumour cells but not normal lung fibroblasts. Our findings indicate that ATR inhibition could be a promising therapeutic strategy in p53-deficient lung tumours.
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      PubDate: 2017-11-28T08:56:01Z
      DOI: 10.1016/j.jab.2017.10.008
  • Inotodiol protects PC12 cells against injury induced by oxygen and glucose

    • Authors: Yan Li; Wenting Zhang; Chun Chen; Chunping Zhang; Jingyu Duan; Huankai Yao; Qunli Wei; Aiguo Meng; Jun Shi
      Abstract: Publication date: Available online 27 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Yan Li, Wenting Zhang, Chun Chen, Chunping Zhang, Jingyu Duan, Huankai Yao, Qunli Wei, Aiguo Meng, Jun Shi
      Ischemic stroke is a severe cause of disability and death all over the world. To search for effective therapy for ischemic stroke, PC12 cells damaged by oxygenation and glucose deprivation/restoration were employed to assess the protective effects of inotodiol. As a result, inotodiol can improve the cell viability and attenuate the leakage of lactate dehydrogenase. Meanwhile, inotodiol can prevent oxidative stress by reducing reactive oxygen species generation, decreasing the content of malonic dialdehyde, and increasing the activity of superoxide dismutase. In addition, the dysfunction of mitochondria induced by oxygenation and glucose deprivation/restoration was ameliorated through decreasing the level of intracellular calcium and increasing the mitochondrial membrane potential. At the same time, inotodiol can inhibit PC12 cells apoptosis through downregulation of Caspase-3 and Bax as well as upregulation of Bcl-2. These results reveal inotodiol can protect PC12 cells against the injury induced by oxygenation and glucose deprivation/restoration. This investigation gives promising evidences for the therapy of ischemic stroke.
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      PubDate: 2017-11-28T08:56:01Z
      DOI: 10.1016/j.jab.2017.11.004
  • Apigenin inhibits ethanol-induced oxidative stress and LPS-induced
           inflammatory cytokine production in cultured rat hepatocytes

    • Authors: Feng Wang; Rui-Jun Zhou; Xi Zhao; Hua Ye; Mei-Lin Xie
      Abstract: Publication date: Available online 27 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Feng Wang, Rui-Jun Zhou, Xi Zhao, Hua Ye, Mei-Lin Xie
      Apigenin is a natural flavonoid compound that has antioxidative, anti-inflammatory, and hepatoprotective effects, but the underlying mechanisms are still unclear. In this study, the effects of apigenin on ethanol-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammatory cytokine production were examined in cultured rat hepatocytes. Following pretreatment of ethanol-stimulated hepatocytes with apigenin 6–24mM for 2h, the levels of cytochrome P450 2E1 (CYP2E1) protein expression and supernatant alanine aminotransferase and malondialdehyde were reduced (P <0.05 or P <0.01), while the activities of glutathione reductase and glutathione peroxidase were increased (P <0.05 or P <0.01). Likewise, the pretreatment of LPS-stimulated hepatocytes with the same concentrations of apigenin could decrease the levels of nuclear factor-κB protein expression and supernatant tumor necrosis factor-α and interleukin-6 (P <0.05 or P <0.01), and increase the level of IκB-α protein expression (P <0.05 or P <0.01). In all of these results, the concentration of 24μM was the most effective. These findings demonstrate that apigenin may exert an inhibitory effect on ethanol-induced oxidative stress and LPS-induced inflammation in the cultured hepatocytes, and its mechanisms may be related to the reduction of CYP2E1 expression, increment of antioxidative ability, and regulation of inflammatory gene expression.
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      PubDate: 2017-11-28T08:56:01Z
      DOI: 10.1016/j.jab.2017.11.006
  • Antibacterial activity of green silver nanoparticles synthesized from
           Anogeissus acuminata against multidrug resistant urinary tract infecting
           bacteria in vitro and host-toxicity testing

    • Authors: Monali P. Mishra; Rabindra N. Padhy
      Abstract: Publication date: Available online 27 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Monali P. Mishra, Rabindra N. Padhy
      Silver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal particles were below 100nm size. The antibacterial activity of AgNPs was monitored by agar-well diffusion method against 11 multidrug resistant (MDR) urinary tract infection (UTI) causing pathogenic bacteria, isolated from clinical samples. At 15μg/ml AgNPs, values of the zone of inhibition (ZI) ranged from 19 to 13 mm, while against the standard antibiotic, gentamicin 30μg/ml ZI ranged from 28 to 20 mm. Host toxicity testing of AgNPs with cultured lymphocytes from human umbilical cord blood in vitro was done; at 3000mg/l AgNPs, 25% of cell death occurred. Thus, the synthesized AgNPs with aqueous leaf extract of A. acuminata could control most MDR UTI bacteria without any toxicity to human lymphocytes.
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      PubDate: 2017-11-28T08:56:01Z
      DOI: 10.1016/j.jab.2017.11.003
  • Biomechanical evaluation of human lumbar spine in spondylolisthesis

    • Authors: Kamil Joszko; Marek Gzik; Wojciech Wolański; Bożena Gzik-Zroska; Edyta Kawlewska
      Abstract: Publication date: Available online 7 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Kamil Joszko, Marek Gzik, Wojciech Wolański, Bożena Gzik-Zroska, Edyta Kawlewska
      One of the least known conditions of the lumbar spine in terms of biomechanics is spondylolisthesis which causes many serious consequences for the patient. This research aimed to perform a mechanical analysis of the origins of spondylolisthesis and its impact on the biomechanics of the lumbar section of the spine. Within the framework of this study, a physiologically model of the lumbar spine was created in the MADYMO software. In the next stage a slip of vertebra L4 was simulated by means of a controlled forward displacement of the vertebral body of vertebra L4. 10 variants of spondylolisthesis (W1–W10) of different degrees were subjected to a biomechanical evaluation. In maximum bending of the physiological spine at an angle of 27° the value of the shear force amounted to 1.9kN, while for the spine affected by spondylolisthesis with slip grade W9 at the maximum bending of 34° the shear force amounted to 5.5kN. It was observed that the lumbar spine with the simulated spondylolisthesis had greater mobility in comparison with the physiological spine, which was shown by maximum bending angles (physiological 27°, W9 34°).

      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.004
  • Antibacterial and cytotoxicity effects of biogenic palladium nanoparticles
           synthesized using fruit extract of Couroupita guianensis Aubl.

    • Authors: Sathishkumar Gnanasekar; Jeyaraj Murugaraj; Divyabharathi Balakrishnan; Varunkumar Krishnamoorthy; Pradeep K Jha; Prabukumar Seetharaman; Ravikumar Vilwanathan; Sivaramakrishnan Sivaperumal
      Abstract: Publication date: Available online 7 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Sathishkumar Gnanasekar, Jeyaraj Murugaraj, Divyabharathi Balakrishnan, Varunkumar Krishnamoorthy, Pradeep K Jha, Prabukumar Seetharaman, Ravikumar Vilwanathan, Sivaramakrishnan Sivaperumal
      Herein, we report a facile route to synthesize palladium nanoparticles (CGPdNPs) using the aqueous fruit extract of C. guianensis Aubl. as a potent biological reducing agent. Reduction of PdCl2 solution into their Nano scale was confirmed with the formation of a black precipitate which gives a reduced absorbance in UV–vis spectroscopy. Fourier transform infrared spectroscopy (FTIR) reveals the active role of phenolic constituents from C. guianensis in reduction and surface functionalization of nanoparticles (NPs). Dynamic light scattering (DLS) and zeta potential analysis confirms the generation of polydispersity highly stable NPs with large negative zeta value (−17.7mV). Interestingly, X-ray Diffraction (XRD) pattern shows that the synthesized CGPdNPs were face centered cubic crystalline in nature. The HRTEM micrographs of CGPdNPs display well-dispersed, spherical NPs in the size ranges between 5 and 15nm with an average of 6nm. It was also noticed that the synthesized CGPdNPs possess an effective antimicrobial activity against different bacterial human pathogens. On the other hand, in vitro cell viability (MTT) assays reveal that the synthesized CGPdNPs exhibited extraordinary anticancer properties. Eventually, hemocompatibility assay depicts the safe nature of synthesized NPs for biomedical application.

      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.001
  • Propolis: The future therapy against Helicobacter pylori-mediated
           gastrointestinal diseases

    • Authors: Ummay Mahfuza Shapla; Md. Jinat Raihan; Md. Asiful Islam; Fahmida Alam; Md. Solayman; Siew Hua Gan; Md. Sakib Hossen; Md. Ibrahim Khalil
      Abstract: Publication date: Available online 7 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Ummay Mahfuza Shapla, Md. Jinat Raihan, Md. Asiful Islam, Fahmida Alam, Md. Solayman, Siew Hua Gan, Md. Sakib Hossen, Md. Ibrahim Khalil
      Helicobacter pylori (H. pylori), which is found in the stomach of approximately 50% of humans, remains there for almost the entire lifetime of the infected individual, leading to various gastrointestinal tract-associated disorders following full-blown infection. Due to the emergence of antibiotic resistance, recurrence and high cost of therapy, most antibiotic-based treatment strategies are not very effective in eradicating H. pylori infections. The quest for an alternative treatment free of these inconveniences is currently in demand. One of the important alternatives is propolis, produced by the honeybee Apis mellifera, which has been used to treat different diseases since it possesses a wide range of biochemical properties. Propolis has been reported as a useful therapeutic regimen against H. pylori, which is an important cause of gastric inflammation, peptic ulcer, gastric cancer, and lymphomas of mucosa-associated lymphoid tissues. Apart from propolis, various active compounds of other natural products have also been confirmed to be effective. This review compiles the scientific evidence of the role of propolis and other natural products against H. pylori-associated gastrointestinal tract-related health complexities by acing as an anti-angiogenic, anti-inflammatory, and antioxidant factor as well as via modulation of enzymatic activities.
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      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.007
  • Antioxidative effects of aqueous extract of broccoli sprouts against
           Triazophos induced hepatic and renal toxicity in female Wistar rats

    • Authors: Dharmender Sharma; Gurinder Kaur Sangha
      Abstract: Publication date: Available online 7 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Dharmender Sharma, Gurinder Kaur Sangha
      Oxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses – 10, 20 and 30mmol – of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE induced OS biomarkers of hepatic and renal toxicity – ALT, AST, urea and creatinine – were noticed in plasma, while catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), lipid peroxidation (LPO) were estimated in liver and kidney along with histological and apoptotic observation. Plasma ALT, AST, urea and creatinine levels along with organ OS parameters as CAT, SOD, GST and GPx were subtly improved in all BE+TZ treated rats. Decreased LPO and reduced apoptosis along with improved histoarchitecture was observed in all BE+TZ treated rats. Present study suggest that the administration of broccoli extract and TZ combination in rats can prevent severe alterations of hepatic and renal biochemical markers and disruptions of histological structure by antioxidative potential of BE from sprouts.
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      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.11.001
  • DMH4, a VEGFR2 inhibitor, effectively suppresses growth and invasion of
           lung cancer cells

    • Authors: Hao Li; Helen L. Ha; Xiaoxu Ding; Chay Bae; Nicky Gazy; Jijun Hao; Li Zhong
      Abstract: Publication date: Available online 7 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Hao Li, Helen L. Ha, Xiaoxu Ding, Chay Bae, Nicky Gazy, Jijun Hao, Li Zhong
      Non-small-cell lung cancer (NSCLC), the most common type of lung cancer, remains the leading cause of cancer death worldwide. Blocking vascular endothelial growth factor (VEGF) signalling is an effective approach to the treatment of NSCLC. Small molecules have been proven to be good resource for discovery of inhibitors of VEGF signalling. DMH4 is a small molecule that we previously developed and demonstrated to have the property of selectively inhibiting VEGF signalling by targeting VEGF receptor 2 (VEGFR2). In this study, we reported that DMH4 can effectively block phosphorylation of VEGFR2 in both H460 and A549 NSCLC cells, which resulted in significant reduction of NSCLC cell viability in a dose-dependent manner, and the growth inhibition (GI50) of DMH4 against H460 and A549 cell lines were 13.27 and 2.75 mm respectively at 24h. Our further studies demonstrated that DMH4 significantly suppressed migration and invasion of A549 and H460 cells, and induced apoptosis in those cells. Therefore, DMH4 as a small molecular VEGFR2 inhibitor may represent a new valuable drug lead for NSCLC treatment.

      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.006
  • A novel algorithm for identifying risk factors for rare events: Predicting
           transient ischemic attack in young patients with low-risk atrial

    • Authors: Chieh-Yu Liu; Hui-Chun Chen
      Abstract: Publication date: Available online 6 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Chieh-Yu Liu, Hui-Chun Chen
      Identification of risk factors for transient ischemic attack (TIA) is crucial for patients with atrial fibrillation (AF). However, identifying risk factors in young patients with low-risk AF is difficult, because the incidence of TIA in such patients is very low, which would result in traditional multiple logistic regression not being able to successfully identify the risk factors in such patients. Therefore, a novel algorithm for identifying risk factors for TIA is necessary. We thus propose a novel algorithm, which combines multiple correspondence analysis and hierarchical cluster analysis and uses the Taiwan National Health Insurance Research Database, a population-based database, to determine risk factors in these patients. The results of this study can help clinicians or patients with AF in preventing TIA or stroke events as early as possible.

      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.005
  • miR-214 and miR-126 were associated with restoration of endothelial
           function in obesity after exercise and dietary intervention

    • Authors: Shen Wang; Jingwen Liao; Junhao Huang; Honggang Yin; Weiyue Yang; Min Hu
      Abstract: Publication date: Available online 4 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Shen Wang, Jingwen Liao, Junhao Huang, Honggang Yin, Weiyue Yang, Min Hu
      Obesity would result in increased cardiovascular morbidity including endothelial destruction, and miRNAs are recognized as potent regulators on endothelial function. We therefore explored pivotal miRNAs before and after exercise and dietary intervention in obese adults and examined their potential relationships with selected endothelial function and biomarkers. Obese adults were included in an exercise and dietary intervention training program for 2 months. At the beginning and the end, measurements of anthropometric and metabolic parameters were performed. Flow-mediated dilation, endothelial related biochemicals and circulating miR-214 and miR-126 levels were also determined. Results showed that circulating miR-214 and miR-126 levels were significantly enhanced (P <0.05) by exercise and dietary intervention along with improved endothelial function. The relationship between relative changes of miR-214 and that of endothelial progenitor cells was significant (r =0.589, P <0.05); relative expression of miR-126 was also significantly (r =0.433, P <0.05) correlated with endothelial nitric oxide synthase. The intervention lead to upregulation of circulating miR-214 and miR-126 in obesity, and these molecular adaptations are associated with improved endothelial function during the restoring process.
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      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.003
  • NETosis − Dr. Jekyll and Mr. Hyde in inflammation

    • Authors: Lucia Lauková; Barbora Konečná
      Abstract: Publication date: Available online 3 November 2017
      Source:Journal of Applied Biomedicine
      Author(s): Lucia Lauková, Barbora Konečná
      Neutrophils play an important role as the central mediators of the innate immune defence response, providing the first line of host protection. It was shown that these cells can trap and kill various microorganisms through different ways. One of them is a release of neutrophil extracellular traps (NETs) composed of chromatin fibrils and antimicrobial proteins. There is the evidence that the release of NETs does not have only a beneficial effect. NETs can trap and kill microorganisms and pathogens, however on the other hand the same pathway can also cause the damage of the organism by various mechanisms. NETs participate in the pathogenesis of a lot of inflammatory and autoimmune disorders, such as thrombosis, atherosclerosis, cystic fibrosis, periodontitis, lupus, rheumatoid arthritis and others. The aim of this review is to summarize information about the release of NETs and their beneficial, but also detrimental effect during various diseases. The better characterization and understanding of the dual role of NETosis during these diseases is necessary for the early diagnosis and more effective treatment.
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      PubDate: 2017-11-09T20:06:45Z
      DOI: 10.1016/j.jab.2017.10.002
  • Novel chromogenic bacteria characterized and their probable treatment
           options using herbal products and reagents to restrict biofilm formation

    • Authors: Sarbani Ashe; Subhadarshani Agasti; Satish Lakkoji; Pradipta Ranjan Rauta; Harekrushna Sahoo; Monalisa Mishra; Bismita Nayak
      Abstract: Publication date: Available online 24 August 2017
      Source:Journal of Applied Biomedicine
      Author(s): Sarbani Ashe, Subhadarshani Agasti, Satish Lakkoji, Pradipta Ranjan Rauta, Harekrushna Sahoo, Monalisa Mishra, Bismita Nayak
      Among all oral diseases, teeth infection is a commonly encountered problem in dentistry with teeth blackening commonly seen in kids. One of the major causes of teeth blackening are the chromogenic bacteria which form biofilm in the teeth. So far there is no significant and safe treatment available for teeth blackening. The current study focuses on teeth blackening by chromogenic bacteria and various factors promoting the biofilm formation and its treatment by an alternative herbal approach. Distinct colonies isolated from dental swabs of children with black stained teeth were grown individually on oyster shell to identify the particular isolate responsible for tooth discoloration. The interaction of traditionally known substances like NaCl, glucose, charcoal powder, mustard oil, KMnO4 and H2O2 on the growth of bacterium and biofilm forming ability were studied. Further, the leaf and stem extracts of Achyranthus aspera and Alternanthera sessilis (available locally) on the growth and biofilm formation was also evaluated. Finally, the efficacy of the various agents was determined by the reduced biofilm formation as well as change in the membrane potential of the isolates. From our results we propose a non-toxic approach comprising herbal compounds and household reagents against teeth blackening to improve oral hygiene.
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      PubDate: 2017-08-25T21:05:03Z
      DOI: 10.1016/j.jab.2017.08.001
  • Film wound dressing with local anesthetic based on insoluble
           carboxymethycellulose matrix

    • Authors: Lenka Vinklárková; Ruta Masteiková; Gabriela Foltýnová; Jan Muselík; Sylvie Pavloková; Jurga Bernatonienė; David Vetchý
      Abstract: Publication date: Available online 23 August 2017
      Source:Journal of Applied Biomedicine
      Author(s): Lenka Vinklárková, Ruta Masteiková, Gabriela Foltýnová, Jan Muselík, Sylvie Pavloková, Jurga Bernatonienė, David Vetchý
      The aim of the presented research was to formulate, prepare and evaluate novel film wound dressings containing lidocaine hydrochloride. The conversion of partially substituted fibrous sodium carboxymethylcellulose (CMC) to an acidic form of CMC enabled the formation of an insoluble matrix which consequently provided the prepared films with excellent handling properties in their wet state. The drug concentration which was incorporated into an external layer of the film was 5mg/cm2. The films demonstrated satisfactory mass and drug content uniformity as well as an acidic surface pH advantageous for wound application. An in vitro drug release test proved that the insoluble CMC matrix served as a reliable carrier without slowing down the release of lidocaine hydrochloride − more than 90% of the drug was released during the first 15min, indicating a quick rate of anesthetic action. The prepared films could be potential wound dressings for comfortable and efficient topical anesthesia before/after procedures on the wound.
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      PubDate: 2017-08-25T21:05:03Z
      DOI: 10.1016/j.jab.2017.08.002
  • RGD peptide doped polypyrrole film as a biomimetic electrode coating for
           impedimetric sensing of cell proliferation and cytotoxicity

    • Authors: Yuan Li; Chao Yu
      Abstract: Publication date: Available online 12 July 2017
      Source:Journal of Applied Biomedicine
      Author(s): Yuan Li, Chao Yu
      A novel and facile in vitro cell sensing system has been developed with one-step electropolymerization of the conducting polypyrrole(PPy) polymer using RGD peptide as the sole dopant on an indium tin oxide (ITO) surface. The resulted RGD peptide-doped polypyrrole (PPy/RGD) composite film had a robust surface, in which PPy provided a biocompatible matrix for cell growth and a conducting interface for electrical detection, while the RGD peptide entrapped in the PPy matrix conferred the desired biomimetic properties. Using the human lung cancer cell A549 as a model, this system can be used to monitor cell behaviors of proliferation and cytotoxicity.
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      PubDate: 2017-07-19T07:59:31Z
      DOI: 10.1016/j.jab.2017.06.001
  • Neuroprotection and cognitive enhancement by treatment with γ-oryzanol in
           sporadic Alzheimer’s disease

    • Authors: Abhishek B. Jha; Shital S. Panchal
      Abstract: Publication date: Available online 16 June 2017
      Source:Journal of Applied Biomedicine
      Author(s): Abhishek B. Jha, Shital S. Panchal
      Sporadic Alzheimer’s disease is characterised by cognitive impairments and associated with cerebrometabolic anomaly and dementia which are burdensome for the old age population and their caregivers worldwide. A safe and effective treatment is essentially required for its prevention and cure. γ-Oryzanol (OZ) is reported for anti-oxidative, anti-inflammatory, anticancer, anti-hyperlipidemic, and anti-diabetic effects in various preclinical studies. In silico studies showed similar binding interactions with acetylcholinisterase like Donepezil (DONO). In vitro DPPH assay, AchE activity inhibition assay and cell viability assay on SH-SY5Y cell line confirmed that OZ has good free radical scavenging and cholinesterase inhibitory activity as well as safety profile. OZ was evaluated in vivo for its effect in streptozotocin (STZ) induced sporadic Alzheimer’s disease in rats. Maze tests reflected improvement in spatial cognitive behavior indicated by reduction in working memory and reference memory error. OZ showed anti-AchE, anti-inflammatory and antioxidative effects. OZ showed effective anti-proinflammatory effects in cerebral milieu as indicated by significant reduction in active glial cells and found to improve synaptic connectivity. Thereby OZ reflected protection of the cerebral architecture against STZ induced damage. Thus, OZ exhibits its candidature as a therapeutic moiety to improve cognitive behavior in neurodegenerative disorders.
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      PubDate: 2017-06-17T10:43:13Z
      DOI: 10.1016/j.jab.2017.05.001
  • Segmentation and detection of physical activities during a sitting task in
           Parkinson’s disease participants using multiple inertial sensors

    • Authors: Sara Memar; Mehdi Delrobaei; Greydon Gilmore; Kenneth McIsaac; Mandar Jog
      Abstract: Publication date: Available online 16 June 2017
      Source:Journal of Applied Biomedicine
      Author(s): Sara Memar, Mehdi Delrobaei, Greydon Gilmore, Kenneth McIsaac, Mandar Jog
      Introduction The development of inertial sensors in motion capture systems enables precise measurement of motor symptoms in Parkinson’s disease (PD). The type of physical activities performed by the PD participants is an important factor to compute objective scores for specific motor symptoms of the disease. The goal of this study is to propose an approach to automatically detect the physical activities over a period time and segment the time stamps for such detected activities. Methods A wearable motion capture sensor system using inertial measurement units (IMUs) was used for data collection. Data from the sensors attached to the shoulders, elbows, and wrists were utilized for detecting and segmenting the activities. An unsupervised machine learning algorithm was employed to extract suitable features from the appropriate sensors and classify the data points to the corresponding activity group. Results The performance of the proposed technique was evaluated with respect to the manually labeled and segmented activities. The experimental results reveal that the proposed auto detection technique – by obtaining high average scores of accuracy (96%), precision (96%), and recall (98%) – is able to effectively detect the activities during the sitting task and segment them to the proper time stamps.

      PubDate: 2017-06-17T10:43:13Z
      DOI: 10.1016/j.jab.2017.05.002
  • Microbial synthesis of nanoparticles and their potential applications in

    • Authors: Anila Fariq; Tabeer Khan; Azra Yasmin
      Abstract: Publication date: Available online 18 April 2017
      Source:Journal of Applied Biomedicine
      Author(s): Anila Fariq, Tabeer Khan, Azra Yasmin
      Nanotechnology is a multidisciplinary field evolved within past few decades and playing substantial role in environment, industry, agriculture and pharmacology. Integration of nanotechnology and biotechnology has led to the foundation of nanomedicine. It has provided novel breakthroughs in cure of various ailments and disorders, drug delivery systems, detection and diagnostics of different diseases. However, use of microbially synthesized nanoparticles in health and medicine is still limited. This article highlights the green approach of nanomaterials synthesis using microbes and current status of their applications in biomedical field.
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      PubDate: 2017-04-26T10:48:37Z
      DOI: 10.1016/j.jab.2017.03.004
  • Ameliorating effect of Curculigo orchoides on chromium(VI) induced
           oxidative stress via, modulation of cytokines, transcription factors and
           apoptotic genes

    • Authors: Navya K.; Phani Kumar G.; Anilakumar K.R.
      Abstract: Publication date: Available online 7 April 2017
      Source:Journal of Applied Biomedicine
      Author(s): Navya K., Phani Kumar G., Anilakumar K.R.
      The effect of hydro-alcoholic extract of Curculigo orchoides on hexavalent chromium (Cr VI) induced toxicity in rats was investigated. Sub-acute toxicity studies were performed by OECD guidelines. K2Cr2O7 (30mg/kg) was administered to all groups except control group for a period of 28days by oral gavage. Control group received distilled water; treatment groups received C. orchoides (25, 50 and 100mg/kg). Cr(VI) administration resulted in up-regulation of serum biochemical parameters such as alanine transaminase, aspartate transaminase, alkaline phosphatase, and tissue biochemical markers viz. lipid peroxidation and protein carbonyl content. C. orchioides (100mg/kg) significantly decreased these enzyme levels. The activities of anti-oxidant enzymes like superoxide dismutase, catalase and glutathione S-transferase were significantly decreased by Cr(VI) administration (50.7%, 43.7% and 37.9%, respectively). Further, mRNA expression studies and histopathology studies confirmed Cr(VI) toxicity. In all cases, C. orchioides promoted significant restoration of enzyme levels in a dose dependent manner. These results suggest the ameliorating effect of C. orchoides on Cr(VI) induced oxidative stress is probably via, modulation of cytokines, transcription factors and apoptotic genes.
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      PubDate: 2017-04-26T10:48:37Z
      DOI: 10.1016/j.jab.2017.03.003
  • Effects of Shen-Fu injection on random skin flap survival in rats

    • Authors: Lin Dingsheng; Wang Long; Jin Zhicheng; Cai Leyi
      Abstract: Publication date: Available online 19 March 2017
      Source:Journal of Applied Biomedicine
      Author(s): Lin Dingsheng, Wang Long, Jin Zhicheng, Cai Leyi
      Objective The aim of this study was to determine the effects of Shen-Fu injection treatment on skin flap survival in an experimental random pattern skin flap model in rats. Materials and methods Ninety male Sprague–Dawley (SD) rats were randomly assigned to the SFI-treated groups and normal saline-treated (control) group. SFI (10 or 20ml/kg) or normal saline (10ml/kg) were administered intraperitoneally once daily. On postoperative day 2, malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor α (TNF-α) and IL-6 were evaluated. Besides, flap survival rate, tissue edema, mean vessel densities, number of neutrophil, flap angiography and vascular endothelial growth factor (VEGF) expression were examined on day 7. Results Compared with the control group, the mean survival area in SFI groups were significantly larger showing a possible dose-dependent effect of SFI. SOD activity was increased significantly, as well as the VEGF expression. MDA level, in the test group, however, was decreased. The hematoxylin and eosin (H&E) stained slices revealed that SFI had the angiogenesis promotion effect, meantime inflammation was clearly inhibited in the SFI groups. Conclusion This study showed that intraperitoneally administered SFI significantly improved random skin flap survival in rats.

      PubDate: 2017-04-26T10:48:37Z
      DOI: 10.1016/j.jab.2017.03.001
  • Deterrence in metabolic and biofilms forming activity of Candida species
           by mycogenic silver nanoparticles

    • Authors: Sabahat Hamid; Shama Zainab; Rani Faryal; Naeem Ali
      Abstract: Publication date: Available online 9 March 2017
      Source:Journal of Applied Biomedicine
      Author(s): Sabahat Hamid, Shama Zainab, Rani Faryal, Naeem Ali
      Candida, a commensal and opportunistic fungal pathogen has been typically known for its biofilm forming ability and device-associated hospital acquired infections in human. The study aimed at exploring the in-vitro anti-biofilm and anti-metabolic activity of AgNPs against C. albicans (n =2), C. tropicalis (n =2) and C. parapsilosis (n =2) isolated from urine samples. Broth dilution method revealed greater than 50% inhibition at 100ppm against Ag NPs in 24h. An overall reduction of 55–86% in biomass (crystal violet staining assay) and 20–73% in metabolic activity (XTT assay) was observed in 24h old biofilms. However, C. albicans proved to be more susceptible to AgNPs compared to C. tropicalis and C. parapsilosis. Scanning Electron Microscopy revealed patchy growth and deterrence in biofilm biomass when Ag NPs were coated on urinary catheter. Furthermore, viable cell counts of Candida were significantly reduced on AgNPs coated catheter compared to control.
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      PubDate: 2017-03-09T23:11:34Z
      DOI: 10.1016/j.jab.2017.02.003
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