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BIOTECHNOLOGY (241 journals)                  1 2 | Last

Showing 1 - 200 of 240 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 16)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 7)
Advances in Regenerative Medicine     Open Access   (Followers: 2)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 11)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 67)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 33)
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 8)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 43)
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 65)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Beitr?ge zur Tabakforschung International/Contributions to Tobacco Research     Open Access   (Followers: 3)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 4)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal   (Followers: 1)
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomarkers in Drug Development     Partially Free   (Followers: 1)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical Glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosensors Journal     Open Access  
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 28)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 6)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 33)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 155)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 5)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 13)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 2)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 10)
Biotechnology Frontier     Open Access   (Followers: 2)
Biotechnology Journal     Hybrid Journal   (Followers: 16)
Biotechnology Law Report     Hybrid Journal   (Followers: 4)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 40)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 10)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
BIOTIK : Jurnal Ilmiah Biologi Teknologi dan Kependidikan     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 16)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 2)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 20)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 4)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 56)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 12)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 3)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription   (Followers: 1)
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 6)
Frontiers in Systems Biology     Open Access   (Followers: 2)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 2)
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Indonesian Journal of Medicine     Open Access  
Industrial Biotechnology     Hybrid Journal   (Followers: 17)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 13)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 4)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
JMIR Biomedical Engineering     Open Access  
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of BioScience and Biotechnology     Open Access  
Journal of Biosecurity Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 64)
Journal of Biotechnology and Strategic Health Research     Open Access   (Followers: 1)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription   (Followers: 1)
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 25)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 17)
Journal of Integrative Bioinformatics     Open Access  
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 12)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 12)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Meat Technology     Open Access  
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 4)
Metalloproteinases In Medicine     Open Access  
Microbial Biotechnology     Open Access   (Followers: 10)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access   (Followers: 1)
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  
Nanobiotechnology     Hybrid Journal   (Followers: 2)
Nanomaterials and Nanotechnology     Open Access  
Nanomedicine and Nanobiology     Full-text available via subscription  
Nanomedicine Research Journal     Open Access  

        1 2 | Last

Journal Cover
Journal of Applied Biomedicine
Journal Prestige (SJR): 0.348
Citation Impact (citeScore): 2
Number of Followers: 2  

  This is an Open Access Journal Open Access journal
ISSN (Print) 1214-021X - ISSN (Online) 1214-0287
Published by Elsevier Homepage  [3162 journals]
  • Berbamine protects the heart from isoproterenol induced myocardial
           infarction by modulating eNOS and iNOS expressions in rats

    • Abstract: Publication date: Available online 7 June 2018Source: Journal of Applied BiomedicineAuthor(s): Saranya Sithuraj, Vijaya Padma Viswanadha AimThe current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat’s heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.Graphical abstractGraphical abstract for this article
  • Adipocytokines and new onset diabetes mellitus after transplantation

    • Abstract: Publication date: Available online 31 May 2018Source: Journal of Applied BiomedicineAuthor(s): Dominika Goldmannová, Jaromíra Spurná, Ondřej Krystyník, Jan Schovánek, Lubica Cibičková, David Karásek, Josef Zadražil Background and aimDiabetes mellitus is a very common metabolic disease with a rising incidence. It is one of the most serious comorbidities in renal transplant recipients. New-onset diabetes after renal transplantation (NODAT) is associated with poor graft function, higher rates of cardiovascular complications and a poor prognosis. Adipocytokines, synthetized by adipose tissue influence metabolic pathways and disorders in various ways. In this review article, we chose the most researched adipocytokines and evaluated their relationship to posttransplant diabetes mellitus. The aim of this paper is to summarize current knowledge and discuss their perspective role in diagnostics or therapy of the new onset diabetes mellitus after transplantation.Graphical abstractGraphical abstract for this article
  • Red American ginseng enhances the effect of fluorouracil on human colon
           cancer cells via both paraptosis and apoptosis pathways

    • Abstract: Publication date: Available online 31 May 2018Source: Journal of Applied BiomedicineAuthor(s): Jin-Yi Wan, Haiqiang Yao, Chun-Feng Zhang, Wei-Hua Huang, Qihui Zhang, Zhi Liu, Yi Bi, Stephanie Williams, Chong-Zhi Wang, Chun-Su Yuan IntroductionAs a commonly used chemotherapeutic agent, fluorouracil (5-FU) has serious dose-limiting side effects. In this study, we evaluated the synergy between red American ginseng (RAG) and 5-FU on human colorectal cancer cells, and explored the potential mechanisms.MethodsGinsenoside contents of white American ginseng (WAG) and RAG were determined by HPLC. Cell proliferation was evaluated by MTS assay. Combination Index (CI) analysis was executed using CompuSyn software. Paraptotic events were observed after crystal violet staining. Cell cycle distribution, cyclin A expression and apoptotic induction were analyzed using flow cytometry.ResultsWe observed the heat treatment remarkably increased levels of ginsenoside Rg3, 20R-Rg3, Rk1 and Rg5. When the combinations of 5-FU and RAG were applied, cell proliferation inhibition rates were notably increased, indicating that RAG significantly enhanced 5-FU’s effect. Additionally, CI analysis suggested that there was a synergistic action of 5-FU and RAG when combined. The cell cycle data indicated 5-FU induced S phase arrest, and the combination of 5-FU and RAG increased G1 phase. Further, the RAG’s ability to enhance the anti-cancer effects of 5-FU was linked to both paraptosis and apoptosis inductions.ConclusionRAG may have clinical utility to decrease the dosage of 5-FU in colorectal cancer therapeutics.Graphical abstractGraphical abstract for this article
  • Effect of Pseuduvaria macrophylla in attenuating hyperglycemia mediated
           oxidative stress and inflammatory response in STZ-nicotinamide induced
           diabetic rats by upregulating insulin secretion and glucose transporter-1,
           2 and 4 proteins expression

    • Abstract: Publication date: Available online 26 May 2018Source: Journal of Applied BiomedicineAuthor(s): Hairin Taha, Aditya Arya, Ataul Karim Khan, Nayiar Shahid, Mohammed Ibrahim Bin Noordin, Syam Mohan Pseuduvaria macrophylla (Family: Annonaceae) is commonly used as medicinal plant in Malaysia. A recent study with the Pseuduvaria species showed antioxidant and antidiabetic effects. This study aimed to ascertain antidiabetic potential of methanolic extract of Pseuduvaria macrophylla bark (PM) using streptozotocin-nicotinamide induced diabetic rat models. Various phytochemical and biochemical properties of the plant have been evaluated. The results showed that the extract has potentially normalized the elevated blood glucose levels by upregulating the insulin and C-peptide levels and alleviated oxidative stress by improving glutathione (GSH) and reducing lipid peroxidation (LPO) in the diabetic rats. In addition, PM has drastically downregulated the levels of pro-inflammatory cytokines and transforming growth factor beta-1 (TGF-β1). Histopathological examination of the pancreas in PM treated diabetic rats showed significant recovery of the pancreatic structural degeneration and thus reflected the protective role of PM against peroxidation damage by a rise in insulin level as evidenced by the immunohistochemistry study. The improved expressions of GLUT-1, GLUT-2 and GLUT-4 further confirmed the restoration of β-cell mass by PM. Interestingly, the findings demonstrated the antioxidant, anti-inflammatory and antihyperglycemic potential of PM which may provide future lead for the management of type-2 diabetes.Graphical abstractGraphical abstract for this article
  • A proteomic glimpse into the oncogenesis of prostate cancer

    • Abstract: Publication date: Available online 24 May 2018Source: Journal of Applied BiomedicineAuthor(s): Patrícia Borba Martiny, Diego Duarte Alcoba, Brasil Silva Neto, Paulo Costa Carvalho, Ilma Simoni Brum Prostate cancer (PCa) is the second most frequent cancer in men worldwide. Distinguishing between the nonaggressive and aggressive forms of this disease is difficult, and a means to better characterize molecular patterns that could aid in diagnosis is urgently needed. Here, we compare the proteomic profiles of PCa and benign prostatic hyperplasia (BPH) in an effort to elucidate underlying mechanisms of oncogenesis. We compared protein expression in PCa and BPH tissue biopsies using quantitative tandem mass tag (TMT) and a MultiNotch data acquisition proteomic on an Orbitrap Fusion. Four proteins that were observed to be differentially abundant in the mass spectrometry analysis were selected for further comparison with quantitative real-time PCR: S100A4, L-lactate dehydrogenase B-chain (LDHB), Phosphatidylethanolamine-binding-protein 1-RAF (RKIP), and Ras suppressor-protein-1 (RSU1). Mass spectrometry showed RKIP and RSU1 to be upregulated in PCa, while S100A4 and LDHB were upregulated in BPH. q-PCR results were in agreement with quantitative proteomic data in BPH tissue but disagree with gene expression analysis of PCa samples. These four elements showed higher gene expression in BPH than in PCa. Taken together, our results complement and reinforce the current understanding of prostate cancer progression.Graphical abstractGraphical abstract for this article
  • Effect of different loads of treadmill exercise on Th1/Th2 cytokine
           balance in rat splenocytes

    • Abstract: Publication date: Available online 24 May 2018Source: Journal of Applied BiomedicineAuthor(s): Zahra Gholamnezhad, Mohammad Hossein Boskabady, Mahmoud Hosseini The effect of moderate and overtraining exercise on Th1/Th2 balance was evaluated in rat splenocytes. Male Wistar rats were divided into sedentary control (C), moderately trained (MT; V = 20 m/min, 30 min/day, 8 weeks), overtrained (OT; V = 25 m/min, 60 min/day, 11 weeks) and recovered after overtraining (OR) (OT plus 2 weeks recovery) groups. At the end of study, cell viability, proliferation, interleukin 4 (IL-4) and interferon-γ (IFN-γ) secretion were evaluated in non-stimulated, phytohemagglutinin (PHA) and concavaline A (Con A)-stimulated splenocytes. Cell viability increased in MT and OR groups compared to control. Cell proliferation was higher in OR group than other groups. IL-4 concentration in PHA-stimulated cells from MT and OT groups, and IL-4 concentration in Con A-stimulated cells from OR and OT groups, were higher than the control group, but not for IFN-γ. In non-stimulated cells, IFN-γ/ IL-4 ratio was higher than MT and OT groups. In PHA and Con A-stimulated cells, IFN-γ/ IL-4 ratio was lower in exercise groups than control. We previously showed that moderate exercise increases Th1 cytokines in serum, but in splenocytes, Th2 or Th1 response may increase depending on the type of mitogen stimulation. Two-week recovery restored Th1/Th2 balance, only in non-stimulated splenocytes of overtrained animals.Graphical abstractGraphical abstract for this article
  • In vitro antimalarial activity of synthesized TiO2 nanoparticles using
           Momordica charantia leaf extract against Plasmodium falciparum

    • Abstract: Publication date: Available online 8 May 2018Source: Journal of Applied BiomedicineAuthor(s): Pachiyappan Rajiv Gandhi, Chidambaram Jayaseelan, Chinnaperumal Kamaraj, S.R. Radhika Rajasree, Rathinasamy Regina Mary Malaria is a serious global health challenge, and it has infected millions of people worldwide. There is an urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. In the present study, we biosynthesized TiO2 nanoparticles (NPs) using the Momordica charantia leaf aqueous extract as a reducing and stabilizing agent. TiO2 NPs were characterized by UV, XRD, FTIR, HRTEM, EDX, DLS and Zeta-potential. The maximum activity of mosquitocidal was observed in the synthesized TiO2 NPs against Anopheles stephensi Liston (Diptera: Culicidae) larvae and pupae, LC50 were 2.50 mg/l (I instar), 2.86 mg/l (II), 3.29 mg/l (III), 3.43 mg/l (IV), and 5.04 mg/l (pupa). The antimalarial activity of M. charantia leaf aqueous extract and TiO2 NPs were evaluated against CQ-resistant (CQ-r) and CQ sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of M. charantia leaf aqueous extract were 83.64 μg/ml (CQ-s) and 88.14 μg/ml (CQ-r). Synthesized TiO2 NPs achieved IC50 of 53.42 μg/ml (CQ-s) and 59.71 μg/ml (CQ-r). The TiO2 NPs did not exhibit any noticeable toxicity on Poecilia reticulata after 24 h of exposure. Overall, our results suggest that the synthesized TiO2 NPs may be employed to develop newer and safer agents for malaria control.Graphical abstractGraphical abstract for this article
  • Propolis: The future therapy against Helicobacter pylori-mediated
           gastrointestinal diseases

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Ummay Mahfuza Shapla, Jinat Raihan, Asiful Islam, Fahmida Alam, Naeem Solayman, Siew Hua Gan, Sakib Hossen, Ibrahim Khalil Helicobacter pylori (H. pylori), which is found in the stomach of approximately 50% of humans, remains there for almost the entire lifetime of the infected individual, leading to various gastrointestinal tract-associated disorders following full-blown infection. Due to the emergence of antibiotic resistance, recurrence and high cost of therapy, most antibiotic-based treatment strategies are not very effective in eradicating H. pylori infections. The quest for an alternative treatment free of these inconveniences is currently in demand. One of the important alternatives is propolis, produced by the honeybee Apis mellifera, which has been used to treat different diseases since it possesses a wide range of biochemical properties. Propolis has been reported as a useful therapeutic regimen against H. pylori, which is an important cause of gastric inflammation, peptic ulcer, gastric cancer, and lymphomas of mucosa-associated lymphoid tissues. Apart from propolis, various active compounds of other natural products have also been confirmed to be effective. This review compiles the scientific evidence of the role of propolis and other natural products against H. pylori-associated gastrointestinal tract-related health complexities by acing as an anti-angiogenic, anti-inflammatory, and antioxidant factor as well as via modulation of enzymatic activities.Graphical abstractGraphical abstract for this article
  • Nonlinear Heart Rate Variability based artificial intelligence in lung
           cancer prediction

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Reema Shyamsunder Shukla, Yogender Aggarwal Lung cancer is uncontrolled growth of cells that occurs due to exposure to smoke, radiation and chemicals, which causes chronic stress and associated with impaired autonomic nervous system. Nonlinear heart rate variability (HRV) analysis has been suggested to uncover the performance status of lung cancer subjects and distinguish them from healthy controls. The present work obtained tachogram from recorded electrocardiogram of 104 lung cancer subjects and 30 healthy controls to extract HRV indices. The obtained results suggested lowered HRV (altered autonomic nervous system tone) values from Eastern Cooperative Oncology Group (ECOG) 1 to ECOG4. Subject males had higher HRV measures than their female counterparts. The HRV parameters decreased from ECOG PS of 1 to 4. Control females had higher HRV measures than control males. There was no association between age and HRV measures. Statistically, nonlinear HRV features were observed significant. ANN exhibited ECOG1 83.3%, ECOG2 50%, ECOG3 90%, ECOG4 95% and Controls 86.7%. The prediction analysis using artificial neural network (ANN) and support vector machine (SVM) scoring an accuracy of 93.09% and 100% with nonlinear HRV indices as input thus has been suggested to be a tool of prognostic importance.
  • Caveolin-1 rs4730751 gene polymorphism in kidney allograft recipients

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Sylwia Słuczanowska-Głąbowska, Damian Malinowski, Krzysztof Safranow, Leszek Domański, Michał Czerewaty, Przemysław Ustianowski, Maria Laszczyńska, Andrzej Pawlik Caveolin-1 is a phosphoprotein that plays a crucial role in tissue remodeling and fibrotic pathways, controlling fibroblast function, proliferation, and apoptosis. Here we aimed to examine the association between the CAV1 rs4730751 gene polymorphism and kidney graft function, graft histology, and graft survival. This study enrolled 270 Caucasian deceased – donor renal transplant recipients. We found no statistically significant associations between CAV1 rs4730751 and delayed graft function, acute rejection, or chronic allograft dysfunction, as well as creatinine values at 1–60 months after transplantation, the risk of graft loss, dialysis or death after transplantation, or histopathological changes in kidney allograft biopsies. Our findings do not support an association between the CAV1 gene rs4730751 polymorphism and kidney allograft function in our population.Graphical abstractGraphical abstract for this article
  • Inotodiol protects PC12 cells against injury induced by oxygen and glucose

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Yan Li, Wenting Zhang, Chun Chen, Chunping Zhang, Jingyu Duan, Huankai Yao, Qunli Wei, Aiguo Meng, Jun Shi Ischemic stroke is a severe cause of disability and death all over the world. To search for effective therapy for ischemic stroke, PC12 cells damaged by oxygenation and glucose deprivation/restoration were employed to assess the protective effects of inotodiol. As a result, inotodiol can improve the cell viability and attenuate the leakage of lactate dehydrogenase. Meanwhile, inotodiol can prevent oxidative stress by reducing reactive oxygen species generation, decreasing the content of malonic dialdehyde, and increasing the activity of superoxide dismutase. In addition, the dysfunction of mitochondria induced by oxygenation and glucose deprivation/restoration was ameliorated through decreasing the level of intracellular calcium and increasing the mitochondrial membrane potential. At the same time, inotodiol can inhibit PC12 cells apoptosis through downregulation of Caspase-3 and Bax as well as upregulation of Bcl-2. These results reveal inotodiol can protect PC12 cells against the injury induced by oxygenation and glucose deprivation/restoration. This investigation gives promising evidences for the therapy of ischemic stroke.Graphical abstractGraphical abstract for this article
  • Antibacterial activity of green silver nanoparticles synthesized from
           Anogeissus acuminata against multidrug resistant urinary tract infecting
           bacteria in vitro and host-toxicity testing

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Monali P. Mishra, Rabindra N. Padhy Silver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal particles were below 100 nm size. The antibacterial activity of AgNPs was monitored by agar-well diffusion method against 11 multidrug resistant (MDR) urinary tract infection (UTI) causing pathogenic bacteria, isolated from clinical samples. At 15 μg/ml AgNPs, values of the zone of inhibition (ZI) ranged from 19 to 13 mm, while against the standard antibiotic, gentamicin 30 μg/ml ZI ranged from 28 to 20 mm. Host toxicity testing of AgNPs with cultured lymphocytes from human umbilical cord blood in vitro was done; at 3000 mg/l AgNPs, 25% of cell death occurred. Thus, the synthesized AgNPs with aqueous leaf extract of A. acuminata could control most MDR UTI bacteria without any toxicity to human lymphocytes.Graphical abstractGraphical abstract for this article
  • In vivo assessment of time dependent changes of T2* in medial meniscus
           under loading at 3T: A preliminary study

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Lenka Hornakova, Vladimir Juras, Petr Kubovy, Daniel Hadraba, David Gerych, Pavel Stursa, Xeni Deligianni, Oliver Bieri, Siegfried Trattnig, Karel Jelen Due to the internal structure of the knee joint, the ability to characterize and quantify the dynamic response of the meniscal tissue directly in vivo is highly problematic. The main purpose of this study was to investigate the behaviour of the meniscus under loading conditions. Four healthy young females were included. To obtain T2* values in the meniscus, the vTE sequence was used with 10 echoes ranging from 0.8 to 10.1 ms. Submilisecond first echo time is a great advantage of vTE sequence allowing for precise mapping of relatively short T2*. The two-parametric least squares fitting procedure was used to calculate T2* pixel-wise. A custom-made diamagnetic apparatus was developed to simulate stress conditions on the lower limb in a conventional MR scanner. vTE T2* was performed in five consecutive scans, 6:10 min apart. Three different compartments of the medial and lateral meniscus were segmented. The differences at the different time-points were calculated. A constant increase of T2* times after compression was statistically significant in the anterior horn of the medial meniscus. T2* mapping with variable echo time sequence might be a satisfactorily sensitive technique to detect the changes of meniscus physiology under loading conditions in vivo.Graphical abstractEvaluation of the meniscal response to the load by MRI. An example of a resulting mono-exponential T2* map for each time-point (comp0–comp4) in the medial meniscus. Evaluation of similar sections of medial meniscus without loading (A = comp0) and with loading (B = comp1; C = comp2; D = comp3; E = comp4). A constant increase of T2* times after compression was statistically significant in the anterior horn of the medial meniscus.Graphical abstract for this article
  • Effect of pramipexole alginate nanodispersion (PAND) on the transgenic
           Drosophila expressing human alpha synuclein in the brain

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, Alim Hussain Naqvi In the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3 μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress markers and dopamine content was also observed in the PD flies exposed to various doses PAND. The improvement in the PD symptoms was more in PD flies exposed to PAND compared to PD flies exposed to pramipexole alone.Graphical abstractGraphical abstract for this article
  • Antioxidative effects of aqueous extract of broccoli sprouts against
           Triazophos induced hepatic and renal toxicity in female Wistar rats

    • Abstract: Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Dharmender Sharma, Gurinder Kaur Sangha Oxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses – 10, 20 and 30 mmol – of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30 days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE induced OS biomarkers of hepatic and renal toxicity – ALT, AST, urea and creatinine – were noticed in plasma, while catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), lipid peroxidation (LPO) were estimated in liver and kidney along with histological and apoptotic observation. Plasma ALT, AST, urea and creatinine levels along with organ OS parameters as CAT, SOD, GST and GPx were subtly improved in all BE + TZ treated rats. Decreased LPO and reduced apoptosis along with improved histoarchitecture was observed in all BE + TZ treated rats. Present study suggest that the administration of broccoli extract and TZ combination in rats can prevent severe alterations of hepatic and renal biochemical markers and disruptions of histological structure by antioxidative potential of BE from sprouts.Graphical abstractGraphical abstract for this article
  • Therapeutic potential of rice-derived polyphenols on obesity-related
           oxidative stress and inflammation

    • Abstract: Publication date: Available online 20 March 2018Source: Journal of Applied BiomedicineAuthor(s): Esther T. Callcott, Abishek B. Santhakumar, Jixun Luo, Christopher L. Blanchard Global obesity rates are of epidemic proportion. With limited treatments available there is a large demand for therapeutic alternatives. Polyphenols derived from coloured rice varieties may serve as a potential functional food alternative in combating obesity and obesity-related diseases. The antioxidant and anti-inflammatory properties of polyphenols found in coloured rice varieties could have the ability to neutralize oxidative stress and modulate inflammatory responses in obese populations. This review discusses polyphenols derived from rice, the oxidative stress and inflammatory pathways involved in obesity pathogenesis, bioavailability of polyphenols and the therapeutic potential of polyphenols on transcriptional and molecular pathways related to obesity and obesity-related diseases.Graphical abstractGraphical abstract for this article
  • Topic application of meloxicam-loaded polymeric nanocapsules as a
           technological alternative for treatment of the atopic dermatitis in mice

    • Abstract: Publication date: Available online 20 March 2018Source: Journal of Applied BiomedicineAuthor(s): Douglas Mroginski Weber, Guilherme Teixeira Voss, Renata Leivas de Oliveira, Caren A.R. da Fonseca, Jaini Paltian, K.C. Rodrigues, Francine Rodrigues Ianiski, R.A. Vaucher, Cristiane Luchese, Ethel Antunes Wilhelm This study investigated the effect of the topical treatment with meloxicam-loaded nanocapsules (M-NC) on symptoms, inflammatory response and oxidative parameters in an atopic dermatitis (AD) model in BALB/c mice. 2,4-Dinitrochlorobenzene (DNCB) was applied to the dorsal skin on days 1–3 for sensitization. Mice were challenged with DNCB on the ear (on days 14–29) and dorsal skin (on days 14, 17, 20, 23, 26, and 29). Treatments with blank nanocapsules (B-NC), free meloxicam (M-F) or M-NC were applied to the backs of the mice from days 14 to 29. On the day 30, skin severity scores and scratching behaviour were determined. After that, ears and dorsal skin were removed for determination of inflammatory parameters (edema and myeloperoxidase (MPO) activity) and oxidative parameters (thiobarbituric acid reactive species (TBARS) and non-protein thiol (NPSH) levels), respectively. DNCB increased the severity of skin lesions, scratching behaviour, edema and MPO activity of ears and dorsal skin TBARS levels. M-NC reversed skin severity scores, scratching behaviour and inflammatory response induced by DNCB. B-NC and M-F did not have effect in this model. In summary, meloxicam carried by polymeric nanocapsules reversed inflammatory response and ameliorated symptoms in an AD model.Graphical abstractGraphical abstract for this article
  • Chemopreventive action of non-steroidal anti-inflammatory drugs in
           9,10-dimethylbenzanthracene induced lung carcinogenesis in BALB/C mice:
           Expression of COX-1, COX-2 and Nf-κB

    • Abstract: Publication date: Available online 20 March 2018Source: Journal of Applied BiomedicineAuthor(s): Ravi Kishore Saini, Sankar Nath Sanyal, Jasvinder Singh Bhatti Non-steroidal anti-inflammatory drugs (NSAIDs) play an effective chemopreventive action against a variety of cancers. The present study aimed at targeting pro-inflammatory cyclooxygenase (COX) and NF-kB mediated inflammatory pathways in 9,10-dimethylbenzanthracene (DMBA) induced lung cancer in BALB/C mice and chemoprotective action of NSAIDs. Animals were divided into five groups and treated with NSAIDs, intratracheally, daily for a period of 18 weeks. Group 1 as control, received vehicle treatment; Group 2 received single dose of DMBA (10 mg/kg bw); Group 3, 4 and 5 besides DMBA treatment, also received Aspirin (60 mg/kg bw), Celecoxib (6.0 mg/kg bw) and Etoricoxib (0.6 mg/kg bw), respectively. DMBA induce DNA damage, apoptosis and expression of COX 1, COX 2 and NF-κB using immunofluorescence and blot analysis were done. The present study demonstrated the formation of micronuclei, over-expression of COX-2 and NF-κB in DMBA induced lung tumorigenesis and thereby suggesting a marked role of inflammation in the tumour progression. Results indicate the formation of micronuclei in DMBA group, which were significantly reduced in aspirin treated group, and totally absent in the celecoxib and etoricoxib groups. In conclusion, co-administration of etoricoxib and celecoxib has significantly reduced the inflammatory potential of the growing neoplasm in DMBA induced lung cancer in male BALB/C mice.Graphical abstractGraphical abstract for this article
  • Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles
           with antimicrobial, antioxidant and cytotoxic activities

    • Abstract: Publication date: Available online 12 March 2018Source: Journal of Applied BiomedicineAuthor(s): Annu, Shakeel Ahmed, Gurpreet Kaur, Praveen Sharma, Sandeep Singh, Saiqa Ikram Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV–vis spectroscopy, Dynamic light scattering, and High Resolution Transmission Electron Microscopy. TEM studies revealed 9–46 nm size range of synthesized AgNPs. The antimicrobial and antioxidant activities were also studied by using Agar well diffusion method and DPPH Assay, respectively. Nanoparticles showed good antimicrobial activity against both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. Further, bioactivity assays revealed selective cytotoxicity (anticancer) of the nanoparticles against human lung cancer cell line A549. The nanoparticles are able to induce cancer cell specific apoptosis at G0/G1 phase of cell cycle. The results showed potential mechanism of action of nanoparticles via augmentation of antioxidant system in cancer cells. Over all, this study show multifaceted potential bioactivities of nanoparticles generated from fruit waste.Graphical abstractGraphical abstract for this article
  • d-glucose-induced+injuries+through+upregulation+of+microRNA-30d-5p+level+in+human+AC16+cardiac+cells&rft.title=Journal+of+Applied+Biomedicine&rft.issn=1214-021X&">Naringenin alleviates high d-glucose-induced injuries through upregulation
           of microRNA-30d-5p level in human AC16 cardiac cells

    • Abstract: Publication date: Available online 2 March 2018Source: Journal of Applied BiomedicineAuthor(s): Jiamei Jiang, Guobiao Liang, Zijun Wu, Hailiang Mo, Qiong You, Zhiqiang Wang, Keng Wu, Runmin Guo As a common complication of diabetes mellitus (DM), diabetic cardiomyopathy (DCM) is considered to be one of the major causes of mortality and morbidity. The therapeutic effects of naringenin have been verified in the treatment of various human diseases. However, the application of naringenin in the treatment of DCM still has not been reported. In this study, human AC16 cardiac cells were treated with normal d-glucose and high d-glucose (HG). After transfection with miR-30d-5p inhibitor, Cell Counting Kit-8 (CCK-8) method was used to measure cell viability. Hoechst 33258 staining was performed to observe the morphological changes of nucleus. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the activity of caspase-3. Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. Levels of light chain 3 (LC3) including LC3-I and LC3-II as well as nucleoporin p62 (P62) were detected by Western blot. We found that Naringenin treatment increased the reduced cell variability caused by HG treatment. Naringenin also increased expression level of miR-30d-5p in human AC16 cardiac cells after HG treatment. Treatment with miR-30d-5p inhibitor reduced the effect of miR-30d-5p in increasing cell variability and reducing cell apoptosis. Naringenin treatment reduced the increased levels of LC-I, LC-II and P62, but miR-30d-5p inhibitor reduced those changes. Therefore we concluded that naringenin could alleviate HG-induced injuries through the upregulation of microRNA-30d-5p level in human AC16 cardiac cells.
  • Genetic association of Tumour necrosis factor alpha, Interleukin-18 and
           Interleukin 1 beta with the risk of coronary artery disease: A
           case-control study outcome from Kashmir

    • Abstract: Publication date: Available online 27 February 2018Source: Journal of Applied BiomedicineAuthor(s): Nuzhat Shaheen Khan, Mohammad Sultan Allai, Bushra Nissar, Niyaz Ahmad Naykoo, Iqra Hameed, Misbah Majid, Aaliya Bhat, Falaque ul Afshan, Bashir Ahmad Ganai Coronary artery disease (CAD) is a clinical manifestation of atherosclerosis in the arteries supplying myocardium. Inflammation is the cornerstone in the development and progression of atherosclerosis. Amongst the various biomolecules tumour necrosis factor-α (TNF-α), interleukin-18 (IL-18) and interleukin-1β (IL-1β) build an inflammatory bionetwork in developing the disease. In this study we investigated the association of TNF-α SNPs [–308G/A (rs1800629), −1031T/C (rs1799964), −863C/A (rs1800630)]; IL-18 [–137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), −31C/T (rs1143627), and −511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the −308G/A-A/A and −863A/A showed an association with disease while −1031T/C was found to have an inverse relation. The IL-18–137G/C showed no statistically significant difference between controls and cases. For IL-1β the +3954C/T and −31C/T SNP variants showed no disease association while −511T/T showed significant association. Haplotypic analysis revealed the haplotype ATCGCC and GTACCTC to be associated with CAD risk and GTCGTTT, in particular, showing a profound association. Overall, our study suggests that TNF-α and IL-1β promoter polymorphisms may act as genetic risk factors in developing the coronary artery disease.Graphical abstractGraphical abstract for this article
  • RIG-1 and MDA5 are the important intracellular sensors against bacteria in
           septicemia suffering patients

    • Abstract: Publication date: Available online 21 February 2018Source: Journal of Applied BiomedicineAuthor(s): Asieh Asadpour-Behzadi, Ashraf Kariminik BackgroundMDA5 and RIG-1 are the important intracellular receptors which detect microbial associated molecular patterns. Septicemia is a condition in which infection enters the bloodstream of the patients. The main intracellular mechanisms against septicemia are yet to be clarified. Therefore, this research study was aimed to evaluate expression of MDA5 and RIG-1 in the patients suffering from septicemia in comparison to healthy controls.MethodsMDA5 and RIG-1 expression levels in 40 patients suffering from septicemia and 40 healthy controls were evaluated using Real-Time PCR technique. The sources of bacteria in the bloodstream of the patients suffering from septicemia were determined using microbial cultures.ResultsThe results showed that mRNA levels of MDA5 and RIG-1 were significantly increased in the patients when compared to healthy controls. The results also revealed that the patients were infected with four bacteria including Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii and Pseudomonas aeruginosa. mRNA levels of MDA5 and RIG-1 did not differ among patients with various bacterial infections.ConclusionBased on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity.Graphical abstractGraphical abstract for this article
  • Lectin-based analysis of human milk immunoglobulin G fucosylated variants
           in relation to milk maturation and perinatal risk factors

    • Abstract: Publication date: Available online 13 February 2018Source: Journal of Applied BiomedicineAuthor(s): Jolanta Lis-Kuberka, Magdalena Orczyk-Pawiłowicz, Barbara Królak-Olejnik, Marta Berghausen-Mazur, Karolina Barańska, Iwona Kątnik-Prastowska BackgroundFucosylated glycotopes of milk immunoglobulin G (IgG) are ligands in reactions of biological recognition protecting newborns against infection and ensuring proper development.Materials and methodsRelative amounts of IgG fucosyl-glycovariants in milk of mothers giving birth to term and premature newborns (term and preterm milk groups) were analysed by lectin-IgG-ELISA using α1,2-, α1,3-, and α1,6-fucose specific biotinylated Ulex europaeus (UEA), Tetragonolobus purpureus (LTA), and Lens culinaris (LCA) lectins, respectively.ResultsThe term and preterm milk IgG glycovariants were highly reactive with UEA, LTA, and LCA, whereas maternal plasma IgG poorly or at all. During milk maturation the IgG of very preterm and preterm milk compared to term milk differed by lower relative amounts of UEA-, higher of LTA-, and nearly stable expression of LCA-reactive glycotopes. Moreover, lower α1,2- and higher α1,3- relative amounts of lectin-dependent milk IgG-fucosylated glycovariants were found to be associated with an infectious disease of lactating mothers.ConclusionThe highly fucosylated glycovariants of human IgG given with mothers’ milk to immunologically immature newborn seem to be bifunctional molecules with potential therapeutic properties. The analysis of fucosylation status of milk IgG by simple lectin-IgG-ELISA may be helpful to control the immunological quality of milk for milk banking.Graphical abstractGraphical abstract for this article
  • JNK inhibitor CC-930 reduces fibrosis in a murine model of
           Nf1-deficient fracture repair

    • Abstract: Publication date: Available online 13 February 2018Source: Journal of Applied BiomedicineAuthor(s): Nikita Deo, Jad El-Hoss, Mille Kolind, Kathy Mikulec, Lauren Peacock, David G. Little, Aaron Schindeler Tibial pseudarthrosis often features deficient bone formation, excessive bone resorption, and extensive pathological fibrosis, particularly in individuals with Neurofibromatosis type I (NF1). It was hypothesized that overactive NF1-Ras-JNK signalling may underlie the pathological fibrosis, and that this could be treated via a JNK antagonist. CC-930, a small molecule JNK inhibitor, was trialed in closed fractures in wild type mice CC-930 (25 mg/kg/twice daily) was dosed throughout fracture healing (D2–21) and during the latter stages of repair (D11–21). All fractures healed by D21, regardless of treatment, with some of the CC-930 (D11–21) treatment group showing early bridging. CC-930 (D11–21) was tested in an Nf1-null fracture model where Nf1 was inactivated by Ad-Cre virus injection in Nf1flox/flox mice; these mice also possessed a Cre-responsive tdTomato transgene. CC-930 resulted in a significant decrease in non-unions (93% vehicle vs. 64% CC-930, p 
  • Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and
           sulfate metabolism of Desulfovibrio piger

    • Abstract: Publication date: Available online 10 February 2018Source: Journal of Applied BiomedicineAuthor(s): Ivan Kushkevych, Monika Vítězová, Jiří Kos, Peter Kollár, Josef Jampílek An increased number of sulfate-reducing bacteria is often isolated from faeces of patients with gastrointestinal diseases, which can be the cause of the development of bowel inflammation. Frequent use of antibiotics causes the resistance of intestinal microorganisms and ineffective treatment of these diseases. The antimicrobial activity and biological properties of the selected ring-substituted 8-hydroxyquinoline-2-carboxanilides against Desulfovibrio piger Vib-7 were studied. The addition of these compounds in the cultivation medium inhibited the bacterial growth and the process of sulfate reduction dose-dependently. A significant cytotoxic activity under the influence of ring-substituted 8-hydroxyquinoline-2-carboxanilides was determined. The strongest cytotoxic effect of the derivatives was observed for compounds 8-hydroxy-N-(3-methoxyphenyl)quinoline-2-carboxamide and 8-hydroxy-N-(3-trifluoromethylphenyl)quinoline-2-carboxamide that caused a low survival of D. piger Vib-7 in concentration 17 μM and high toxicity rates.Graphical abstractGraphical abstract for this article
  • Antioxidant and anti-inflammatory mechanisms of polyphenols isolated from
           virgin coconut oil attenuate cadmium-induced oxidative stress-mediated
           nephrotoxicity and inflammation in rats

    • Abstract: Publication date: Available online 10 February 2018Source: Journal of Applied BiomedicineAuthor(s): Ademola C. Famurewa, Abumchukwu J. Ejezie, Chioma S. Ugwu-Ejezie, Ebele J. Ikekpeazu, Fidelis E. Ejezie PurposeCadmium (Cd) is a classic cumulative nephrotoxicant and literature suggests that its toxicity is associated with oxidative stress and inflammation which contribute to pathologies in various tissues. We sought to investigate whether polyphenols isolated from virgin coconut oil (VCO) would modulate nephrotoxicity and inflammation induced by Cd in rats.MethodsRats were administered polyphenols prior to and along with Cd (5 mg/kg, orally) for 7 weeks. Serum markers of renal damage, interleukin-6 (IL-6), C-reactive protein (CRP) and nitric oxide (NO) were evaluated; renal activities of antioxidant enzymes, as well as malondialdehyde (MDA) and reduced glutathione (GSH) content were determined. Histopathologic alterations were evaluated to define kidney damage.ResultsCadmium exposure induced nephrotoxicity and oxidative stress evident by significantly increased serum levels of creatinine, urea, and uric acid along with remarkable depression in renal activities of antioxidant enzymes and GSH with prominent increase in MDA. Inflammatory markers – IL-6, CRP and NO were significantly increased and confirmed by histopathology. Sub-chronic administration of VCO polyphenols attenuated the Cd-induced biochemical alterations compared to Cd control with remarkably improved histopathological observations.ConclusionThe findings showed that VCO polyphenol supplementation protects against Cd-induced nephrotoxicity via its antioxidant and anti-inflammatory mechanisms in rats.Graphical abstractGraphical abstract for this article
  • Cell-free nucleic acids in urine as potential biomarkers of kidney disease

    • Abstract: Publication date: Available online 9 February 2018Source: Journal of Applied BiomedicineAuthor(s): Marianna Gyurászová, Alexandra Kovalčíková, Janka Bábíčková, Július Hodosy, Ľubomíra Tóthová Kidney and uropoetic system diseases represent a major social, economic and health burden. This is mainly because early diagnosis of kidney dysfunction is currently unavailable, since the current markers are often reliably increased only after advanced progression of the renal diseases. Recently, circulating nucleosomes, DNA and numerous forms of RNA have been detected in human biological fluids, such as plasma, urine, saliva, and breast milk. Although their biological functions remain mostly unknown, they are attractive as potential biomarkers of various diseases. In urine, many of the circulating nucleic acids originate from the cells of the kidney and the urinary tract making these non-invasive and easily obtained new biomarkers in the nephrology or urology. This review focuses on cell free nucleic acids in urine and its potential in human studies. Although, there are some technical and biological limitations, the urinary circulating nucleic acids hold a great potential as new biomarkers of renal diseases.Graphical abstractGraphical abstract for this article
  • Induction of caspase-mediated apoptosis using Alnus japonica extracts in
           AGS human gastric carcinoma cells

    • Abstract: Publication date: Available online 9 February 2018Source: Journal of Applied BiomedicineAuthor(s): Seong-Eun Kim, Yon-Suk Kim, Woen-Bin Shin, Jin-Su Park, Sang-Ho Moon, Byong-Tae Jeon, Pyo-Jam Park Alnus japonica has been used as a traditional oriental medicine for many diseases such as fever, haemorrhage and alcoholism. In this study, A. japonica extracts were evaluated for their in vitro antioxidant potentials and anticancer effects in AGS human gastric carcinoma cell line. The antioxidant properties of A. japonica extracts were evaluated using several biochemical assays, including FRAP (ferric reducing antioxidant power) assay, ABTS (2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), alkyl and hydroxyl radical scavenging activity assay. Our study showed that ethanol extract of A. japonica (AJE) has a more potent antioxidant activity than its water extract. In addition, AJE extract inhibited the cell growth and induced the cell death by increasing reactive oxygen species (ROS) production in AGS cells. Moreover, AJE extract specifically triggered the apoptosis mediated through the activation of caspase-8, 7, 3, and poly-ADP ribose polymerase (PARP). Thus, these results suggest that AJE extract could be potentially useful as a new promising strategy in the therapy for gastric carcinoma cancer.Graphical abstractGraphical abstract for this article
  • Quantification of uPA in breast tumour tissue extracts by microarray
           immunoassay: Comparison with ELISA technology

    • Abstract: Publication date: Available online 3 February 2018Source: Journal of Applied BiomedicineAuthor(s): Liu Shi, Thomas Gehin, Yann Chevolot, William Jacot, Pierre-Jean Lamy, Emmanuelle Laurenceau The urokinase-type plasminogen activator (uPA) and PA inhibitor 1 (PAI-1) play important roles in breast cancer metastasis through cell migration and invasion. They are clinically applicable prognostic and predictive markers. High levels of uPA and PAI-1 are associated with high risk of recurrence and adjuvant chemotherapy provides substantial benefit for this breast cancer population. The current sole validated method for quantifying uPA level in breast tumour tissue is ELISA assay. It requires 50–300 mg of fresh or frozen tissue, which is the main limitation for routine use. In this study, we evaluated the performances of customized antibody microarray to quantify uPA concentration from reduced extraction solution of breast tumour tissue and compared it with standard ELISA kit. We firstly optimized the elaboration of customized antibody microarray in order to sensitively detect and quantify uPA standard solutions. In the best conditions, we analysed uPA concentration in 16 cytosolic extracts from breast tumour tissue. Results showed that our customized antibody microarray could correctly quantify uPA concentration while consuming 100 times less volume of tumour tissue extraction solution than ELISA. Our antibody microarray is a powerful and promising tool for the miniaturization of the immunoassay quantification of uPA from breast tumour tissue extracts.Graphical abstractGraphical abstract for this article
  • The influence of modulators of acetylcholinesterase on the resistance of
           mice against soman and on the effectiveness of antidotal treatment of
           soman poisoning in mice

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Jiri Kassa, Jan Korabecny, Eugenie Nepovimova, Daniel Jun The potency of one reversible inhibitor of acetylcholinesterase (6-chlorotacrine), one reactivator of acetycholinesterase (K027) and their combination to increase the resistance of mice against soman and the efficacy of antidotal treatment of soman-poisoned mice was evaluated. While 6-chlorotacrine was able to markedly protect mice against acute toxicity of soman and the pharmacological pretreatment with 6-chlorotacrine increased the efficacy of antidotal treatment (the oxime HI-6 in combination with atropine) of soman-poisoned mice more than two times, the bispyridinium oxime K027 did not protect mice from acute toxicity of soman, however, the pharmacological pretreatment with this compound was able to markedly increase the efficacy of antidotal treatment of soman-poisoned mice. On the other hand, the combination of both modulators of acetylcholinesterase did not increase the prophylactic efficacy of 6-chlorotacrine alone. These findings demonstrate that pharmacological pretreatment of soman-poisoned mice can be promising and useful in the case of administration of 6-chlorotacrine while the administration of the oxime K027 did not bring any additional benefit when combined with 6-chlorotacrine.Graphical abstractGraphical abstract for this article
  • NETosis − Dr. Jekyll and Mr. Hyde in inflammation

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Lucia Lauková, Barbora Konečná Neutrophils play an important role as the central mediators of the innate immune defence response, providing the first line of host protection. It was shown that these cells can trap and kill various microorganisms through different ways. One of them is a release of neutrophil extracellular traps (NETs) composed of chromatin fibrils and antimicrobial proteins. There is the evidence that the release of NETs does not have only a beneficial effect. NETs can trap and kill microorganisms and pathogens, however on the other hand the same pathway can also cause the damage of the organism by various mechanisms. NETs participate in the pathogenesis of a lot of inflammatory and autoimmune disorders, such as thrombosis, atherosclerosis, cystic fibrosis, periodontitis, lupus, rheumatoid arthritis and others. The aim of this review is to summarize information about the release of NETs and their beneficial, but also detrimental effect during various diseases. The better characterization and understanding of the dual role of NETosis during these diseases is necessary for the early diagnosis and more effective treatment.Graphical abstractGraphical abstract for this article
  • IL-37 mediates the anti-tumor activity in non-small cell lung cancer
           through IL-6/STAT3 pathway

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Yan-Ming Deng, Hua Zhang, Jian-Miao Liang, Hai-Bing Xian, Ze-Cheng Chen, Yi-Cong Tang, Shuang Yang, Wei-Neng Feng The occurrence and development of lung cancer is closely related to inflammation. Thus, we conducted the present study to investigate the effects of IL-37 (Interleukin 37), a newly identified anti-inflammatory factor, on non-small cell lung cancer (NSCLC), which accounts for about 85% of all lung cancers. To address the function of IL-37 in NSCLC, we first evaluated IL-37 expression in the human NSCLC tissues; then the IL-37 function was assessed in vitro and in vivo in a xenografted lung tumor model. IL-37 was barely expressed in the NSCLC tissue but highly expressed in the adjacent normal tissue. This expression profile was validated by ELISA (Enzyme-linked immunoassay), western blot and immunohistochemical staining. Recombinant IL-37 could suppress cell migration, invasion and proliferation and promote cell apoptosis in NSCLC cell line A549 and SK-MES-1. IL-37 inhibited the IL-6/STAT3 pathway and also the downstream targets Bcl-2, NEDD9 and Cyclin D1. Overexpressing IL-6 or constitutive active STAT3 eliminated the anti-tumor effects of IL-37. Furthermore, IL-37 expression in vivo could inhibit the cancer development. Our results showed that IL-37 plays an inhibitory role in lung cancer development, possibly through IL-6/STAT3 pathway.Graphical abstractGraphical abstract for this article
  • Proliferation of Toxoplasma gondii (RH strain) is inhibited by the
           combination of pravastatin and simvastatin with low concentrations of
           conventional drugs used in toxoplasmosis

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Raquel Arruda Sanfelice, Larissa Rodrigues Bosqui, Suelen Santos da Silva, Milena Menegazzo Miranda-Sapla, Luciano Aparecido Panagio, Italmar Teodorico Navarro, Ivete Conchon-Costa, Wander Rogério Pavanelli, Ricardo Sergio Almeida, Idessania Nazareth Costa Toxoplasma gondii, an etiologic agent of toxoplasmosis, is an obligate intracellular parasite, which exhibits an apicoplast organelle which assists in the metabolism of isoprenoids and other pivotal mediators for the parasite survival. Statins are drugs that inhibit cholesterol synthesis, blocking the conversion of the substrate HMG-CoA to mevalonate, thus preventing the initial processes of the biosynthesis of these precursors, both in humans and parasite. In the light of this information, we determined the effect of pravastatin and simvastatin associated with the current drugs (pyrimethamine and sulfadiazine) as a possible alternative treatment for this infection. Cytotoxicity was evaluated in HeLa cells by MTT assay, which was observed the drug combinations did not affect cell viability. HeLa cells (105) were infected with T. gondii tachyzoites of RH strain (5 × 105) and treated with pravastatin and/or simvastatin combined with pyrimethamine and/or sulfadiazine for 24 h. Our data showed a significant reduction in cell adhesion, infection and mainly parasite proliferation in all treatments. Based on these results, the combination of statins with drugs used in current therapy showed to be a promising therapeutic alternative for toxoplasmosis treatment.Graphical abstractGraphical abstract for this article
  • miR-214 and miR-126 were associated with restoration of endothelial
           function in obesity after exercise and dietary intervention

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Shen Wang, Jingwen Liao, Junhao Huang, Honggang Yin, Weiyue Yang, Min Hu Obesity would result in increased cardiovascular morbidity including endothelial destruction, and miRNAs are recognized as potent regulators on endothelial function. We therefore explored pivotal miRNAs before and after exercise and dietary intervention in obese adults and examined their potential relationships with selected endothelial function and biomarkers. Obese adults were included in an exercise and dietary intervention training program for 2 months. At the beginning and the end, measurements of anthropometric and metabolic parameters were performed. Flow-mediated dilation, endothelial related biochemicals and circulating miR-214 and miR-126 levels were also determined. Results showed that circulating miR-214 and miR-126 levels were significantly enhanced (P 
  • A novel algorithm for identifying risk factors for rare events: Predicting
           transient ischemic attack in young patients with low-risk atrial

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Chieh-Yu Liu, Hui-Chun Chen Identification of risk factors for transient ischemic attack (TIA) is crucial for patients with atrial fibrillation (AF). However, identifying risk factors in young patients with low-risk AF is difficult, because the incidence of TIA in such patients is very low, which would result in traditional multiple logistic regression not being able to successfully identify the risk factors in such patients. Therefore, a novel algorithm for identifying risk factors for TIA is necessary. We thus propose a novel algorithm, which combines multiple correspondence analysis and hierarchical cluster analysis and uses the Taiwan National Health Insurance Research Database, a population-based database, to determine risk factors in these patients. The results of this study can help clinicians or patients with AF in preventing TIA or stroke events as early as possible.
  • DMH4, a VEGFR2 inhibitor, effectively suppresses growth and invasion of
           lung cancer cells

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Hao Li, Helen L. Ha, Xiaoxu Ding, Chay Bae, Nicky Gazy, Jijun Hao, Li Zhong Non-small-cell lung cancer (NSCLC), the most common type of lung cancer, remains the leading cause of cancer death worldwide. Blocking vascular endothelial growth factor (VEGF) signalling is an effective approach to the treatment of NSCLC. Small molecules have been proven to be good resource for discovery of inhibitors of VEGF signalling. DMH4 is a small molecule that we previously developed and demonstrated to have the property of selectively inhibiting VEGF signalling by targeting VEGF receptor 2 (VEGFR2). In this study, we reported that DMH4 can effectively block phosphorylation of VEGFR2 in both H460 and A549 NSCLC cells, which resulted in significant reduction of NSCLC cell viability in a dose-dependent manner, and the growth inhibition (GI50) of DMH4 against H460 and A549 cell lines were 13.27 and 2.75 mm respectively at 24 h. Our further studies demonstrated that DMH4 significantly suppressed migration and invasion of A549 and H460 cells, and induced apoptosis in those cells. Therefore, DMH4 as a small molecular VEGFR2 inhibitor may represent a new valuable drug lead for NSCLC treatment.
  • Biomechanical evaluation of human lumbar spine in spondylolisthesis

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Kamil Joszko, Marek Gzik, Wojciech Wolański, Bożena Gzik-Zroska, Edyta Kawlewska One of the least known conditions of the lumbar spine in terms of biomechanics is spondylolisthesis which causes many serious consequences for the patient. This research aimed to perform a mechanical analysis of the origins of spondylolisthesis and its impact on the biomechanics of the lumbar section of the spine. Within the framework of this study, a physiologically model of the lumbar spine was created in the MADYMO software. In the next stage a slip of vertebra L4 was simulated by means of a controlled forward displacement of the vertebral body of vertebra L4. 10 variants of spondylolisthesis (W1–W10) of different degrees were subjected to a biomechanical evaluation. In maximum bending of the physiological spine at an angle of 27° the value of the shear force amounted to 1.9 kN, while for the spine affected by spondylolisthesis with slip grade W9 at the maximum bending of 34° the shear force amounted to 5.5 kN. It was observed that the lumbar spine with the simulated spondylolisthesis had greater mobility in comparison with the physiological spine, which was shown by maximum bending angles (physiological 27°, W9 34°).
  • Antibacterial and cytotoxicity effects of biogenic palladium nanoparticles
           synthesized using fruit extract of Couroupita guianensis Aubl.

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Sathishkumar Gnanasekar, Jeyaraj Murugaraj, Balakrishnan Dhivyabharathi, Varunkumar Krishnamoorthy, Pradeep K Jha, Prabukumar Seetharaman, Ravikumar Vilwanathan, Sivaramakrishnan Sivaperumal Herein, we report a facile route to synthesize palladium nanoparticles (CGPdNPs) using the aqueous fruit extract of C. guianensis Aubl. as a potent biological reducing agent. Reduction of PdCl2 solution into their nano scale was confirmed with the formation of a black precipitate which gives a reduced absorbance in UV–vis spectroscopy. Fourier transform infrared spectroscopy (FTIR) reveals the active role of phenolic constituents from C. guianensis in reduction and surface functionalization of nanoparticles (NPs). Dynamic light scattering (DLS) and zeta potential analysis confirms the generation of polydispersed highly stable NPs with large negative zeta value (−17.7 mV). Interestingly, X-ray Diffraction (XRD) pattern shows that the synthesized CGPdNPs were face centered cubic crystalline in nature. The HRTEM micrographs of CGPdNPs displays well-dispersed, spherical NPs in the size ranges between 5 and 15 nm with an average of 6 nm. It was also noticed that the synthesized CGPdNPs possess an effective antimicrobial activity against different bacterial pathogens. On the other hand, in vitro cell viability (MTT) assays reveals that the synthesized CGPdNPs exhibited an extraordinary anticancer properties. Eventually, hemocompatibility assay depicts the safe nature of synthesized NPs for biomedical application.
  • DNA repair inhibitors as radiosensitizers in human lung cells

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Kamila Ďurišová, Lucie Čecháková, Petr Jošt, Zuzana Šinkorová, Adéla Kmochová, Jaroslav Pejchal, Martin Ondrej, Jiřina Vávrová, Aleš Tichý The aim of this study was to compare the effects of DNA repair inhibitors in the context of radio-sensitization of human lung cells. The radio-sensitizing effects of NU7441 (1 mM), an inhibitor of DNA-dependent protein kinase (DNA-PK); KU55933 (10 μM), an inhibitor of ataxia-telangiectasia mutated kinase (ATM); and VE-821 (10 μM), an inhibitor of ATM-related kinase (ATR) were tested by the xCELLigence system for monitoring proliferation, fluorescence microscopy for DNA damage detection, flow-cytometry for cell cycle and apoptosis analysis and western blotting and ELISA for determination of DNA repair proteins. We employed normal human lung fibroblasts (NHLF, p53-wild-type) and non-small cell lung cancer cells (H1299, p53-negative). DNA-PK inhibition (by NU7441) in combination with ionizing radiation (IR) increased the number of double strand breaks (DSB), which persisted 72 h after irradiation in both cell lines. Additionally, NU7441 and KU55933 in combination with IR caused G2-arrest. ATR inhibitor (VE-821) together with IR markedly inhibited proliferation and induced G2/M arrest accompanied by apoptosis in H1299, but not in NHLF cells, and thus diminished DNA-repair of tumour cells but not normal lung fibroblasts. Our findings indicate that ATR inhibition could be a promising therapeutic strategy in p53-deficient lung tumours.Graphical abstractGraphical abstract for this article
  • Apigenin inhibits ethanol-induced oxidative stress and LPS-induced
           inflammatory cytokine production in cultured rat hepatocytes

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Feng Wang, Rui-Jun Zhou, Xi Zhao, Hua Ye, Mei-Lin Xie Apigenin is a natural flavonoid compound that has antioxidative, anti-inflammatory, and hepatoprotective effects, but the underlying mechanisms are still unclear. In this study, the effects of apigenin on ethanol-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammatory cytokine production were examined in cultured rat hepatocytes. Following pretreatment of ethanol-stimulated hepatocytes with apigenin 6–24 mM for 2 h, the levels of cytochrome P450 2E1 (CYP2E1) protein expression and supernatant alanine aminotransferase and malondialdehyde were reduced (P 
  • Sinomenine inhibits the growth of glioma cells through STAT3 signal

    • Abstract: Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Yi-ting Wei, Qun-ying Yang, Shuang-bo Fan, Liang-Wang, Dong-Xia Hu, Ping Shuai The present study was designed to examine effects of Sinomenine (SM) on glioma cells growth in vivo and in vitro. Cells growth and apoptosis were detected by MTT assay, TUNEL assay and flow cytometric analysis. In the study, SM treatment led to growth inhibition on a series of glioma cell lines, including U87, U373, U251, Hs683 and T98G. SM prevented U87 growth in the nude mice as well. Inhibitory effects of SM on U87 cells proliferation in vitro and in vivo were more effective than that of temozolomide (TMZ), and SM has synergistic effects with TMZ in the glioma therapy. SM induced apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Moreover, we observed SM decreased the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. Interestingly, using a specific activator of STAT3, we demonstrated overexpression of p-STAT3 impaired, SM mediated growth inhibition and apoptosis induction in the U87 cells. In summary, our results indicate SM induced growth suppression of human glioma cells through inhibiting phosphorylation of STAT3.Graphical abstractGraphical abstract for this article
  • Oral melatonin administration improves the objective and subjective sleep
           quality, increases 6-sulfatoxymelatonin levels and total antioxidant
           capacity in patients with fibromyalgia

    • Abstract: Publication date: Available online 1 February 2018Source: Journal of Applied BiomedicineAuthor(s): M. Yolanda Castaño, Maria Garrido, Jonathan Delgado-Adámez, Sara Martillanes, M. Ángeles Gómez, Ana Beatriz Rodríguez Background/objectiveChronic pain, sleep disturbances and oxidative stress are implicated in the pathogenesis of fibromyalgia. The aim of this study was to assess the effect of melatonin supplementations on sleep quality, 6-sulfatoxymelatonin (aMT6-s) levels, as well as urinary and serum total antioxidant capacity (TAC) in patients with fibromyalgia.MethodsThirty three patients carried out the full study. A baseline period (10 days) was included in the experimental design to collect information about patients’ initial status. Then, patients took different doses of melatonin, during 10 consecutive days each. Placebo was given during 10 days either before or between melatonin doses. Objective sleep quality was recorded by actigraphy whereas subjective sleep quality was measured by The Pittsburgh Sleep Quality Index. Quantification of aMT6-s and TAC was achieved by ELISA and colorimetric assay kits, respectively.ResultsSix out of seven sleep parameters evaluated by actigraphy were improved after the intake of melatonin as well as the subjective sleep quality. All the biochemical parameters measured were also elevated after the melatonin administration.ConclusionThe daily intake of melatonin improved the sleep quality, increased the aMT6-s levels and the total antioxidant capacity in patients with fibromyalgia.
  • Effect of gender, age, diet and smoking status on the circadian rhythm of
           ascorbic acid (vitamin C) of healthy Indians

    • Abstract: Publication date: Available online 1 February 2018Source: Journal of Applied BiomedicineAuthor(s): Ranjana Singh, Abbas Ali Mahdi, Raj Kumar Singh, Cathy Lee Gierke, Germaine Cornelissen Background/ObjectivesTo determine effects of gender, age, diet, and smoking status on circadian rhythm characteristics of ascorbic acid (vitamin C).Subjects/MethodsAscorbic acid was measured spectrophotometrically in serum collected from 162 healthy volunteers (103 males and 59 females; 7–75 years) every 6 h for 24 h (4 samples). Data were analyzed by single and population mean cosinor. Effects of gender, age, diet (vegetarian vs. omnivore), and smoking status on the rhythm-adjusted mean (MESOR) and circadian amplitude were examined by multiple analysis of variance.ResultsA circadian rhythm is documented with statistical significance by population mean cosinor. In addition to effects of gender and age, the MESOR is affected by diet and smoking status. The circadian amplitude changes nonlinearly as a function of age. The circadian acrophase advances with increasing age.ConclusionThe present observations confirm a definite circadian rhythm in ascorbic acid concentrations with significant effects of age, diet and smoking status in clinical health. Mapping the circadian rhythm of serum ascorbic acid in health can help explore its role in different pathophysiological conditions as pre-disease conditions may be characterized by alterations in the circadian amplitude and/or phase before there is a change in mean value.Graphical abstractGraphical abstract for this article
  • A survey on applying machine learning techniques for management of

    • Abstract: Publication date: Available online 19 January 2018Source: Journal of Applied BiomedicineAuthor(s): Enas M.F. El Houby During the past years, the increase in scientific knowledge and the massive data production have caused an exponential growth in databases and repositories. Biomedical domain represents one of the rich data domains. An extensive amount of biomedical data is currently available, ranging from details of clinical symptoms to various types of biochemical data and outputs of imaging devices. Manually extracting biomedical patterns from data and transforming them into machine-understandable knowledge is a difficult task because biomedical domain comprises huge, dynamic, and complicated knowledge. Data mining is capable of improving the quality of extracting biomedical patterns.In this research, an overview of the applications of data mining on the management of diseases is presented. The main focus is to investigate machine learning techniques (MLT) which are widely used to predict, prognose and treat important frequent diseases such as cancers, hepatitis and heart diseases. The techniques namely Artificial Neural Network, K-Nearest Neighbour, Decision Tree, and Associative Classification are illustrated and analyzed. This survey provides a general analysis of the current status of management of diseases using MLT. The achieved accuracy of the various applications ranged from 70% to 100% according to the disease, the solved problem, and the used data and technique.Graphical abstractGraphical abstract for this articleThe different steps of learning.
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