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BIOTECHNOLOGY (245 journals)                  1 2 | Last

Showing 1 - 200 of 245 Journals sorted alphabetically
3 Biotech     Open Access   (Followers: 8)
Advanced Biomedical Research     Open Access  
Advances in Bioscience and Biotechnology     Open Access   (Followers: 17)
Advances in Genetic Engineering & Biotechnology     Hybrid Journal   (Followers: 9)
Advances in Regenerative Medicine     Open Access   (Followers: 3)
African Journal of Biotechnology     Open Access   (Followers: 6)
Algal Research     Partially Free   (Followers: 10)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 68)
American Journal of Bioinformatics Research     Open Access   (Followers: 7)
American Journal of Polymer Science     Open Access   (Followers: 33)
Amylase     Open Access  
Anadolu University Journal of Science and Technology : C Life Sciences and Biotechnology     Open Access  
Animal Biotechnology     Hybrid Journal   (Followers: 9)
Annales des Sciences Agronomiques     Full-text available via subscription  
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 45)
Applied Biosafety     Hybrid Journal  
Applied Food Biotechnology     Open Access   (Followers: 3)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 67)
Applied Mycology and Biotechnology     Full-text available via subscription   (Followers: 4)
Arthroplasty Today     Open Access   (Followers: 1)
Artificial Cells, Nanomedicine and Biotechnology     Hybrid Journal   (Followers: 1)
Asia Pacific Biotech News     Hybrid Journal   (Followers: 2)
Asian Journal of Biotechnology     Open Access   (Followers: 9)
Asian Pacific Journal of Tropical Biomedicine     Open Access   (Followers: 2)
Australasian Biotechnology     Full-text available via subscription   (Followers: 1)
Banat's Journal of Biotechnology     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 5)
Beitr?ge zur Tabakforschung International/Contributions to Tobacco Research     Open Access   (Followers: 3)
Bio-Algorithms and Med-Systems     Hybrid Journal   (Followers: 2)
Bio-Research     Full-text available via subscription   (Followers: 4)
Bioactive Materials     Open Access   (Followers: 1)
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 4)
Biocybernetics and Biological Engineering     Full-text available via subscription   (Followers: 5)
Bioethics UPdate     Hybrid Journal   (Followers: 1)
Biofuels     Hybrid Journal   (Followers: 11)
Biofuels Engineering     Open Access   (Followers: 1)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 4)
Biological Cybernetics     Hybrid Journal   (Followers: 10)
Biomarkers and Genomic Medicine     Open Access   (Followers: 3)
Biomaterials Research     Open Access   (Followers: 4)
BioMed Research International     Open Access   (Followers: 4)
Biomédica     Open Access  
Biomedical and Biotechnology Research Journal     Open Access  
Biomedical Engineering Research     Open Access   (Followers: 6)
Biomedical Glasses     Open Access  
Biomedical Reports     Full-text available via subscription  
BioMedicine     Open Access  
Biomedika     Open Access  
Bioprinting     Hybrid Journal   (Followers: 1)
Bioresource Technology Reports     Hybrid Journal   (Followers: 1)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 20)
Biosensors Journal     Open Access  
Biosimilars     Open Access   (Followers: 1)
Biosurface and Biotribology     Open Access  
Biotechnic and Histochemistry     Hybrid Journal   (Followers: 1)
BioTechniques : The International Journal of Life Science Methods     Full-text available via subscription   (Followers: 27)
Biotechnologia Acta     Open Access   (Followers: 1)
Biotechnologie, Agronomie, Société et Environnement     Open Access   (Followers: 2)
Biotechnology     Open Access   (Followers: 8)
Biotechnology & Biotechnological Equipment     Open Access   (Followers: 4)
Biotechnology Advances     Hybrid Journal   (Followers: 32)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 158)
Biotechnology and Bioprocess Engineering     Hybrid Journal   (Followers: 6)
Biotechnology and Genetic Engineering Reviews     Hybrid Journal   (Followers: 12)
Biotechnology and Health Sciences     Open Access   (Followers: 1)
Biotechnology and Molecular Biology Reviews     Open Access   (Followers: 2)
Biotechnology Annual Review     Full-text available via subscription   (Followers: 5)
Biotechnology for Biofuels     Open Access   (Followers: 9)
Biotechnology Frontier     Open Access   (Followers: 1)
Biotechnology Journal     Hybrid Journal   (Followers: 17)
Biotechnology Law Report     Hybrid Journal   (Followers: 3)
Biotechnology Letters     Hybrid Journal   (Followers: 34)
Biotechnology Progress     Hybrid Journal   (Followers: 40)
Biotechnology Reports     Open Access  
Biotechnology Research International     Open Access   (Followers: 1)
Biotechnology Techniques     Hybrid Journal   (Followers: 9)
Biotecnología Aplicada     Open Access  
Bioteknologi (Biotechnological Studies)     Open Access  
BIOTIK : Jurnal Ilmiah Biologi Teknologi dan Kependidikan     Open Access  
Biotribology     Hybrid Journal   (Followers: 1)
BMC Biotechnology     Open Access   (Followers: 16)
Cell Biology and Development     Open Access  
Chinese Journal of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Communications in Mathematical Biology and Neuroscience     Open Access  
Computational and Structural Biotechnology Journal     Open Access   (Followers: 1)
Computer Methods and Programs in Biomedicine     Hybrid Journal   (Followers: 8)
Copernican Letters     Open Access   (Followers: 1)
Critical Reviews in Biotechnology     Hybrid Journal   (Followers: 18)
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 3)
Current Bionanotechnology     Hybrid Journal  
Current Biotechnology     Hybrid Journal   (Followers: 3)
Current Opinion in Biomedical Engineering     Hybrid Journal   (Followers: 1)
Current Opinion in Biotechnology     Hybrid Journal   (Followers: 53)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 9)
Current Research in Bioinformatics     Open Access   (Followers: 12)
Current Trends in Biotechnology and Chemical Research     Open Access   (Followers: 2)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 7)
DNA and RNA Nanotechnology     Open Access  
EBioMedicine     Open Access  
Electronic Journal of Biotechnology     Open Access  
Entomologia Generalis     Full-text available via subscription   (Followers: 1)
Environmental Science : Processes & Impacts     Full-text available via subscription   (Followers: 4)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Folia Medica Indonesiana     Open Access  
Food Bioscience     Hybrid Journal  
Food Biotechnology     Hybrid Journal   (Followers: 9)
Food Science and Biotechnology     Hybrid Journal   (Followers: 8)
Frontiers in Bioengineering and Biotechnology     Open Access   (Followers: 5)
Frontiers in Systems Biology     Open Access   (Followers: 1)
Fungal Biology and Biotechnology     Open Access   (Followers: 2)
GM Crops and Food: Biotechnology in Agriculture and the Food Chain     Full-text available via subscription   (Followers: 1)
GSTF Journal of BioSciences     Open Access  
HAYATI Journal of Biosciences     Open Access  
Horticultural Biotechnology Research     Open Access  
Horticulture, Environment, and Biotechnology     Hybrid Journal   (Followers: 11)
IEEE Transactions on Molecular, Biological and Multi-Scale Communications     Hybrid Journal   (Followers: 1)
IET Nanobiotechnology     Hybrid Journal   (Followers: 1)
IN VIVO     Full-text available via subscription   (Followers: 4)
Indian Journal of Biotechnology (IJBT)     Open Access   (Followers: 2)
Indonesia Journal of Biomedical Science     Open Access   (Followers: 2)
Indonesian Journal of Biotechnology     Open Access   (Followers: 1)
Indonesian Journal of Medicine     Open Access  
Industrial Biotechnology     Hybrid Journal   (Followers: 18)
International Biomechanics     Open Access  
International Journal of Bioinformatics Research and Applications     Hybrid Journal   (Followers: 14)
International Journal of Biomechatronics and Biomedical Robotics     Hybrid Journal   (Followers: 4)
International Journal of Biomedical Research     Open Access   (Followers: 2)
International Journal of Biotechnology     Hybrid Journal   (Followers: 5)
International Journal of Biotechnology and Molecular Biology Research     Open Access   (Followers: 4)
International Journal of Biotechnology for Wellness Industries     Partially Free   (Followers: 1)
International Journal of Environment, Agriculture and Biotechnology     Open Access   (Followers: 5)
International Journal of Functional Informatics and Personalised Medicine     Hybrid Journal   (Followers: 4)
International Journal of Life Sciences and Biotechnology     Open Access  
International Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
International Journal of Nanotechnology and Molecular Computation     Full-text available via subscription   (Followers: 3)
International Journal of Radiation Biology     Hybrid Journal   (Followers: 4)
Iranian Journal of Biotechnology     Open Access  
ISABB Journal of Biotechnology and Bioinformatics     Open Access  
Italian Journal of Food Science     Open Access   (Followers: 1)
JMIR Biomedical Engineering     Open Access  
Journal of Biometrics & Biostatistics     Open Access   (Followers: 3)
Journal of Bioterrorism & Biodefense     Open Access   (Followers: 6)
Journal of Petroleum & Environmental Biotechnology     Open Access   (Followers: 1)
Journal of Advanced Therapies and Medical Innovation Sciences     Open Access  
Journal of Advances in Biotechnology     Open Access   (Followers: 5)
Journal Of Agrobiotechnology     Open Access  
Journal of Analytical & Bioanalytical Techniques     Open Access   (Followers: 7)
Journal of Animal Science and Biotechnology     Open Access   (Followers: 4)
Journal of Applied Biomedicine     Open Access   (Followers: 2)
Journal of Applied Biotechnology     Open Access   (Followers: 2)
Journal of Applied Biotechnology Reports     Open Access   (Followers: 2)
Journal of Applied Mathematics & Bioinformatics     Open Access   (Followers: 5)
Journal of Biologically Active Products from Nature     Hybrid Journal   (Followers: 1)
Journal of Biomaterials and Nanobiotechnology     Open Access   (Followers: 6)
Journal of Biomedical Photonics & Engineering     Open Access  
Journal of Biomedical Practitioners     Open Access  
Journal of Bioprocess Engineering and Biorefinery     Full-text available via subscription  
Journal of Bioprocessing & Biotechniques     Open Access  
Journal of BioScience and Biotechnology     Open Access  
Journal of Biosecurity Biosafety and Biodefense Law     Hybrid Journal   (Followers: 3)
Journal of Biotechnology     Hybrid Journal   (Followers: 62)
Journal of Biotechnology and Strategic Health Research     Open Access   (Followers: 2)
Journal of Chemical and Biological Interfaces     Full-text available via subscription   (Followers: 1)
Journal of Chemical Technology & Biotechnology     Hybrid Journal   (Followers: 9)
Journal of Chitin and Chitosan Science     Full-text available via subscription   (Followers: 1)
Journal of Colloid Science and Biotechnology     Full-text available via subscription  
Journal of Commercial Biotechnology     Full-text available via subscription   (Followers: 6)
Journal of Crop Science and Biotechnology     Hybrid Journal   (Followers: 3)
Journal of Ecobiotechnology     Open Access  
Journal of Essential Oil Research     Hybrid Journal   (Followers: 2)
Journal of Experimental Biology     Full-text available via subscription   (Followers: 26)
Journal of Genetic Engineering and Biotechnology     Open Access   (Followers: 5)
Journal of Ginseng Research     Open Access  
Journal of Industrial Microbiology and Biotechnology     Hybrid Journal   (Followers: 18)
Journal of Integrative Bioinformatics     Open Access  
Journal of Medical Imaging and Health Informatics     Full-text available via subscription  
Journal of Molecular Biology and Biotechnology     Open Access  
Journal of Molecular Microbiology and Biotechnology     Full-text available via subscription   (Followers: 13)
Journal of Nano Education     Full-text available via subscription  
Journal of Nanobiotechnology     Open Access   (Followers: 4)
Journal of Nanofluids     Full-text available via subscription   (Followers: 1)
Journal of Organic and Biomolecular Simulations     Open Access  
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Science and Applications : Biomedicine     Open Access  
Journal of the Mechanical Behavior of Biomedical Materials     Hybrid Journal   (Followers: 13)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Journal of Tropical Microbiology and Biotechnology     Full-text available via subscription  
Journal of Yeast and Fungal Research     Open Access   (Followers: 1)
Marine Biotechnology     Hybrid Journal   (Followers: 4)
Meat Technology     Open Access  
Messenger     Full-text available via subscription  
Metabolic Engineering Communications     Open Access   (Followers: 3)
Metalloproteinases In Medicine     Open Access  
Microbial Biotechnology     Open Access   (Followers: 10)
MicroMedicine     Open Access   (Followers: 3)
Molecular and Cellular Biomedical Sciences     Open Access   (Followers: 1)
Molecular Biotechnology     Hybrid Journal   (Followers: 13)
Molecular Genetics and Metabolism Reports     Open Access   (Followers: 3)
Nanobiomedicine     Open Access  

        1 2 | Last

Journal Cover
Biotechnology and Bioprocess Engineering
Journal Prestige (SJR): 0.399
Citation Impact (citeScore): 1
Number of Followers: 6  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1976-3816 - ISSN (Online) 1226-8372
Published by Springer-Verlag Homepage  [2352 journals]
  • Effects of gltA and arcA Mutations on Biomass and 1,3-Propanediol
           Production in Klebsiella pneumoniae
    • Authors: Jung Hun Lee; Hwi-Min Jung; Moo-Young Jung; Min-Kyu Oh
      Abstract: We have previously engineered a Klebsiella pneumoniae strain to increase the 1,3-production (1,3-PDO) yield from glycerol. Here, we describe the further engineering of this strain to improve the biomass formation, resulting in an increase in the 1,3-PDO production. The amino acid lysine at the 167th position in citrate synthase was substituted with alanine using genome editing method to reduce the binding affinity of the enzyme to nicotinamide adenine dinucleotide (NADH). In addition, the arcA gene was deleted that resulted in the inhibition of the expression of citric acid cycle genes under limited aeration conditions. As a consequence, the biomass production was enhanced by 34% and 1,3-PDO formation was elevated from 9.58 to 16.71 g/L. The production of 1,3-PDO per dry cell weight enhanced by 30% from 2.40 to 3.11 g·L−1·DCW−1. The phenotypic changes in the strains were confirmed through the analyses of redox ratio, ATP levels, and changes in the expression of genes related to citric acid cycle and 1,3- PDO pathway.
      PubDate: 2019-01-05
      DOI: 10.1007/s12257-018-0246-0
  • Enhanced Emulsifying and Calcium-binding Properties of Fermented Soybean
    • Authors: Sangkwon Park; Kyung Hoon Chang; Seong Jun Cho
      Abstract: A processed soybean meal (BF-SBM) was prepared by fermenting soybean meal with the genus Bacillus sp. strain for comparison with commercially prepared Lactobacillus-fermented soybean meal (LF-SBM), an enzyme treated soybean meal (ET-SBM) and a soy protein concentrate (SPC). Emulsifying activity and stability of the emulsions prepared from these products were investigated using typical methods in the literature with modifications and Turbiscan measurements, respectively. Calcium ion chelating ability of the SBM products was determined using a modified literature method. The resulting protein profiles, emulsifying properties and calcium-binding behaviors of the soybean products were compared. The results are discussed in terms of the influence of peptide molecular weight and the concentration of specific amino acids on emulsifying and calcium-binding properties of soybean proteins.
      PubDate: 2019-01-05
      DOI: 10.1007/s12257-018-0237-1
  • Development of Biosensor for 3-Hydroxypropionic Acid
    • Authors: Nam Hoai Nguyen; Jung-Rae Kim; Sunghoon Park
      Abstract: In this study, whole-cell biosensors that detect and indicate the concentration of 3-hydroxypropionic acid (3-HP) by producing a green fluorescent signal were developed using a transcription factor (TF)-responsive 3- HP inducible promoter identified in the Pseudomonas denitrificans 3-HP degradation pathway. Upon forming a complex with 3-HP, the MmsR TF protein, a LysR-type transcriptional regulator (LTTR), binds to the PmmsA promoter and controls the expression of the PmmsA-regulated gfp reporter gene in response to the concentration of 3-HP. Furthermore, by enhancing the expression of MmsR and through mutagenesis of the PmmsA promoter region, three well-performing biosensors were developed that covered a wide dynamic range of 3-HP (0.01-100 mM when added externally) with ~100-fold signal change upon induction in P. denitrificans. The 3-HP biosensor machinery, composed of MmsR, the PmmsA promoter and gfp could also function well in E. coli and P. putida. The developed 3-HP biosensors should be useful for engineering 3-HP-producing strains and the enzymes associated with its production.
      PubDate: 2019-01-05
      DOI: 10.1007/s12257-018-0380-8
  • Engineering Escherichia coli to Sense Non-native Environmental Stimuli:
           Synthetic Chimera Two-component Systems
    • Authors: Irisappan Ganesh; Tae Wan Kim; Jeong-Geol Na; Gyeong Tae Eom; Soon Ho Hong
      Abstract: The Two-component Regulatory System (TCS) is the primary mode that bacteria use to continuously sense the environment. A TCS is comprised of a periplasmic sensor Histidine kinase (HK) domain and a cytoplasmic Response regulator (RR) domain. The HK domain phosphorylates the RR domain to activate the effector gene expression. Utilizing a rational approach, the sensor HK was genetically engineered in Escherichia coli to create chimeric HK, by a rewiring or domain swapping strategy. Apart from the wild-type characteristics, chimeric HK imparts novel or the desired characteristics and ability to genetically engineered E. coli for its adaptation and survival. This review focuses on the design, potential applications, and future perspectives of chimeric HKs used as high throughput screening biosensors of various compounds.
      PubDate: 2019-01-05
      DOI: 10.1007/s12257-018-0252-2
  • Esterification of Secondary Alcohols and Multi-hydroxyl Compounds by
           Candida antarctica Lipase B and Subtilisin
    • Authors: Hee-Jeong Cha; Jin-Byung Park; Seongsoon Park
      Abstract: Enzyme-catalyzed esterification of secondary alcohols and multi-hydroxyl compounds is one of the most valuable reactions in organic synthesis. However, it is often difficult to achieve high reaction rates and high regioselectivities with commonly used enzymes such as lipases and proteases. One of the reasons may include bulky substituents of the secondary alcohols and multi-hydroxyl compounds (e.g., carbohydrates and flavonoids). The stereospecificity pocket of lipases, which is considered as a pocket for the binding of medium substituent, might not accept a large substituent due to steric hindrance. Thereby, this review has focused on the discussion about literature survey and structural feature of the most commonly used lipase (i.e., Candida antarctica lipase B (CAL-B)) and serine-protease (i.e., subtilisin) for acylation of secondary alcohols and complex molecules.
      PubDate: 2019-01-05
      DOI: 10.1007/s12257-018-0379-1
  • Production of Lipid Containing High Levels of Docosahexaenoic Acid by
           Cultivation of Aurantiochytrium sp. KRS101 Using Jerusalem Artichoke
    • Authors: Jung-Hyun Ju; Baek-Rock Oh; Seung-Kyu Ryu; Sun-Yeon Heo; Su-Yeon Kim; Won-Kyung Hong; Chul Ho Kim; Jeong-Woo Seo
      Abstract: In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.
      PubDate: 2019-01-02
      DOI: 10.1007/s12257-018-0419-x
  • In vitro Dermal Delivery of Epidermal Growth Factor Using Redox-responsive
    • Authors: Tae Hoon Kim; Dae Sung Yoo; Jin-Chul Kim
      Abstract: Reduction-responsive monoolein (MO) cubosome was prepared for the dermal delivery of epidermal growth factor (EGF) by including cystamine-crosslinked alginate in the water channel of the cubosome. On atomic force micrograph, the cubosome was found as round particles and it was tens of nanometers in diameter. Additives such as alginate, cystamine, and EGF seemed to have little effect on the size and the shape of cubosome. The release of EGF from the cubosome was promoted by dithiothreitol (DTT, a reducing agent), possibly because the disulfide bond of the cross-linker (cystamine) might be broken by the reducing agent. The cubosome promoted the permeation of EGF through an artificial skin mounted on a diffusion cell. Thiazolyl blue tetrazolium bromide (MTT) assay revealed that the cubosome exhibited no marked cellular toxicity after it was incubated with cell (CCD-986sk) for 24 h as long as the concentration was not greater than 20 μg/mL. Free EGF could hardly promote the cell proliferation at all the concentrations tested (0.25 ppm - 1 ppm). EGF contained in the cubosome suspension significantly enhanced cell proliferation when EGF concentration was 0.75 and 1 ppm. EGF contained in the cubosome suspension could maintain about 85% of the initial biological activity while it was stored at 25oC for 4 weeks.
      PubDate: 2019-01-02
      DOI: 10.1007/s12257-018-0383-5
  • An Efficient and Rapid Two-step Purification Method for Active Human
           Macrophage Colony-stimulating Factor from Escherichia coli
    • Authors: Jisu Lee; Jeongmin Lee; Eunha Hwang; Hye-Jung Kim; Sheunghun Lee; Hyerim Lee; Seong Yun Hwang; Sung-Jin Cho; Sun-Ju Yi; Kyunghwan Kim
      Abstract: Macrophage colony-stimulating factor (M-CSF) is a hematopoietic growth factor that stimulates the proliferation and differentiation of mononuclear phagocytes. To be biologically active, M-CSF must form a homodimer linked by disulfide bridges. However, the refolding step of recombinant M-CSF is not only extremely laborious but also rate-limiting. Here, we describe an efficient and simplified method for refolding and purifying M-CSF from Escherichia coli. A truncated M-CSF (amino acids 36–181) was tagged with histidine, expressed, and then purified using nickel affinity columns under denaturing conditions. Our redox refolding buffer containing a mixture of reduced and oxidized glutathione and arginine correctly refolded M-CSF. Chromatography on Q-sepharose columns selectively purified the M-CSF dimer from the monomeric form. The dimeric nature of the purified M-CSF was confirmed using multi angle light scattering combined with high performance liquid chromatography. Moreover, cell proliferation and osteoclast differentiation assays using bone marrow-derived macrophages demonstrated that the recombinant M-CSF was biologically active ex vivo.
      PubDate: 2019-01-02
      DOI: 10.1007/s12257-018-0256-y
  • Discovery of a RuBisCO-like Protein that Functions as an Oxygenase in the
           Novel d -Hamamelose Pathway
    • Authors: Suk Min Kim; Hyun Seung Lim; Sun Bok Lee
      Pages: 490 - 499
      Abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key catalyst of CO2 fixation in nature. RuBisCO forms I, II, and III catalyze CO2 fixation reactions, whereas form IV, also called the RuBisCO-like protein (RLP), is known to have no carboxylase or oxygenase activities. Here, we describe an RLP in Ochrobactrum anthropi ATCC 49188 (Oant_3067; HamA) that functions as an oxygenase in the metabolism of d-hamamelose, a branched-chain hexose found in most higher plants. The d-hamamelose pathway is comprised of five previously unknown enzymes: d-hamamelose dehydrogenase, d-hamamelono-lactonase, d-hamamelonate kinase, d-hamamelonate-2′,5-bisphosphate dehydrogenase (decarboxylating), and the RLP 3-keto-d-ribitol-1,5-bisphosphate (KRBP) oxygenase, which converts KRBP to 3-d-phosphoglycerate and phosphoglycolate. HamA represents the first RLP catalyzing the O2-dependent oxidative C–C bond cleavage reaction, and our findings may provide insights into its applications in oxidative cleavage of organic molecules.
      PubDate: 2018-09-01
      DOI: 10.1007/s12257-018-0305-6
      Issue No: Vol. 23, No. 5 (2018)
  • Isotherm, Kinetic, and Thermodynamic Characteristics for Adsorption of
           2,5-Xylenol onto Activated Carbon
    • Authors: Seo-Hui Park; Jin-Hyun Kim
      Pages: 541 - 549
      Abstract: The adsorption of the major tar compound, 2,5-xylenol, derived from the plant cell cultures of Taxus chinensis, onto activated carbon was examined at different initial 2,5-xylenol concentrations, durations, and temperatures. From the analysis of adsorption isotherms, the Langmuir isotherm model showed good fit to the equilibrium adsorption data. It was found that adsorption capacity decreased with increasing temperature, and the adsorption of 2,5-xylenol onto activated carbon was favorable. The obtained kinetic data for 2,5-xylenol adsorption with activated carbon agreed well with the pseudo-second-order kinetic model. By using intraparticle diffusion model, intraparticle diffusion and boundary layer diffusion did not play a dominant role in 2,5-xylenol adsorption. Thermodynamic parameters were calculated, which indicated that the adsorption was non-spontaneous, irreversible and exothermic nature. The isosteric heat of adsorption decreased with increase in surface loading, indicating a heterogeneous surface.
      PubDate: 2018-09-01
      DOI: 10.1007/s12257-018-0259-8
      Issue No: Vol. 23, No. 5 (2018)
  • Enzymatic Synthesis of Lipophilic Caffeoyl Lipids Using Soybean Oil as the
           Novel Acceptor
    • Authors: Shangde Sun; Liya Tian; Bingxue Hu; Cong Jiang
      Pages: 557 - 563
      Abstract: Soybean oil-based caffeoyl lipids are the novel lipophilic derivatives of caffeic acid, which can be used as UV absorbers and antioxidants in the food and cosmetic industries. In the work, the novel lipophilic structured lipids were prepared using soybean oil as the novel caffeoyl acceptor by enzymatic transesterification. The effects of the reaction variables on the transesterification were investigated, and response surface methodology was used to optimize the reaction variables. Reactions were monitored by HPLC-UV. Different enzymes (Novozym 435, Lipozyme RMIM, and Lipozyme TLIM) were used as biocatalysts, and Novozym 435 showed the best performance for the reaction. The results showed that a high lipophilic soybean oil-based caffeoyl lipids yield (73.5 ± 1.2%) was achieved under the optimal conditions (reaction temperature 85°C, substrate molar ratio 1:6 (ethyl caffeate (EC)/soybean oil), enzyme load 25% (w/w), and 60 h at atmosphere pressure). The activation energies of EC conversion, hydrophilic glyceryl caffeates (GC) and lipophilic caffeoylated acylglycerol (CAG) formations were 32.92 kJ/mol, 17.21 kJ/mol and 57.36 kJ/mol, respectively. Km and Vm were 0.022 mol/L and 0.033 × 10-3 mol/(Lmin), respectively.
      PubDate: 2018-09-01
      DOI: 10.1007/s12257-018-0215-7
      Issue No: Vol. 23, No. 5 (2018)
  • Intergeneric Hybridization between Streptomyces albulus and Bacillus
           subtilis Facilitates Production of ε-Poly-L-lysine from Corn Starch
    • Authors: Shu Li; Nan Wang; Zong-Jun Du; Guan-Jun Chen
      Pages: 580 - 587
      Abstract: Intergeneric hybridization between S.albulus and B. subtilis to produce ε-poly-L-lysine (ε-PL) from corn starch residues (CSR) was investigated in this study. One hybrid, designated S. albulus LS-84, which incorporated the protease gene from B. subtilis, could effectively utilize the protein in CSR as a nitrogen source. In fed-batch fermentation, LS-84 produced 32.6 g/L ε-PL in the presence of 20 g/L CSR. This was an increase of 256.1% compared to that of the parent strain S. albulus LS-01. The rapid hydrolysis of CSR by protease caused rapid growth for LS-84, which allowed higher respiratory activity. As a result, activities of several key enzymes in LS-84 were higher than those in LS-01; additionally, the content of several intracellular amino acids, such as Asp, Glu, and Arg, was also much higher in LS-84. Therefore, intergeneric hybridization between S. albulus and B. subtilis to produce ε-PL from CSR is an economical method for effective utilization of waste resources.
      PubDate: 2018-09-01
      DOI: 10.1007/s12257-018-0253-1
      Issue No: Vol. 23, No. 5 (2018)
  • Exploring Binding Mechanisms between Curcumin and Silkworm 30Kc19 Protein
           Using Spectroscopic Analyses and Computational Simulations
    • Authors: Md. Abdur Razzak; Ji Eun Lee; Hee Ho Park; Tai Hyun Park; Shin Sik Choi
      Pages: 605 - 616
      Abstract: The curry compound, curcumin exerts multiple health-promotive functions; however, its poor solubility and stability limits its biological applications. In this study, we illuminate intermolecular binding mechanisms in the nano-sized complex of curcumin with silkworm protein, 30Kc19. The intrinsic fluorescence of 30Kc19 was gradually quenched by the increase of curcumin concentrations, which demonstrates molecule-molecule complexations mediated by the fluorophore amino acid residues (Tyr, Trp) in the protein. The fluorescence quenching showed that the binding occurred at 1:1 molar ratio with binding constant of 3.28 × 104 M-1. The results from scanning electron microscopy and dynamic light scattering indicate that the complexes were formed with cubicle shapes and sizes of 200–250 nm at pH 8.0 (zeta-potential < −20 mV). Along with Fourier transform infrared analysis, computational studies of protein-ligand docking simulation suggest a mechanism that curcumin and 30Kc19 forms complexes through specific amino acid residues (Trp174, Trp180, and Trp225) with minimum binding distance (4 Å). The complexation of curcumin with 30Kc19 protein effectively suppressed the degradation of curcumin over 10 h and improved its antioxidant activity up to 30%. These findings suggest an application of 30Kc19 for the delivery of waterinsoluble bioactive medicines.
      PubDate: 2018-09-01
      DOI: 10.1007/s12257-018-0285-6
      Issue No: Vol. 23, No. 5 (2018)
  • A Compartmented Microfluidic Reactor for Protein Modification Via
           Solid-phase Reactions - Semi-automated Examination of Two PEGylation
    • Authors: Regina Fraas; Jonas Ferdinand Hübner; Juliane Diehm; Ramona Faas; Rudolf Hausmann; Matthias Franzreb
      Abstract: Microfluidics has emerged as a relatively new scientific field enabling fast reaction times and low demands for reactants. Pursuing these advantages, a compartmented, microfluidic reactor was developed in our group which is suitable for the semi-automated processing of complex reaction cascades including solid phases. As one of the first application examples, we analyzed the influence of different reaction paths on the modification of a model protein in a solid-phase reaction. Extensive characterization experiments were performed: Amongst others, an organic phase was identified which is immiscible with water and compatible with the designated PEGylation reactions. Such organic solvents function as separation plugs for different water based reaction plugs within the microfluidic system. Mixing within the microfluidic system was investigated, in order to ensure an efficient solid-phase reaction. Subsequently, solid-phase PEGylation of the target protein was performed within the microfluidic system via two different reaction cascades. The longest reaction cascades comprised all reactions from particle activation, via protein immobilization and PEGylation to elution and consisted of seven steps. PEGylation in the reactor took place with comparable yields and results as in the control reaction outside the reactor. Due to the modularity, the presented reactor proves to be a versatile instrument for semi-automated reactions and parameter screening, being compatible with biological systems. It combines the advantages of closed channel systems like lab on a chip microfluidics with the flexibility and preparative scale sample volume of larger liquid handling stations.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-017-0322-x
  • A Review of Image Analysis in Biochemical Engineering
    • Authors: Sang-Kyu Jung
      Abstract: The purpose of image analysis is to extract useful information from images. Since modern image analysis allows fast, accurate, and reliable quantitative analysis, it is widely used at present in many areas of research and development. In this article, I review the image analysis methods that are commonly used in biochemical engineering, for which the subjects of the image analysis vary from molecules or cells to whole animals or biomaterials. Images captured by imaging hardware, which is not limited to digital cameras, are processed in multiple steps by applying various image processing algorithms to extract quantitative features. Image analysis has been successfully applied in diverse applications, ranging from simple densitometric evaluation to animal phenotyping and biomass analysis. Although machine learning is poised to become an increasingly common method of image analysis, traditional methods utilizing blob extraction from binarized images are likely to remain in use for the foreseeable future.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0372-8
  • Growth Inhibitory Effect of Mangiferin on Thyroid Cancer Cell Line TPC1
    • Authors: Lei Zhang; Mingchun Wang
      Abstract: Thyroid cancer is the most frequently occurring cancer in human. Therefore, there is a need for better understanding and improvement in the treatment for thyroid tumorigenesis. Many studies show that natural dietary components like Mangiferin (present in mangoes) have anti-carcinogenic ability and reduce the incidence and development of cancers. The present study was focused on analysing the effect of Mangiferin on human thyroid cancer cell line (TPC1) and to assess its therapeutic significance against aggressive thyroid cancers. The cell viability of TPC1 cells was evaluated by MTT assay. The DAPI and AO/Er staining using fluorescence microscopy was used to evaluate the effects of the compound on apoptosis. The PCNA expression was also examined. In the present study, Mangiferin has significantly inhibits TPC1 cell proliferation. DAPI nuclear staining revealed apoptotic changes occurred in nuclei of cells treated with mangiferin (4 μM). AO/EtBr staining of TCP-1 cells showed that mangiferin treatment (2 and 4 μM) induced cell death. Mangiferin induces apoptosis through the induction of cas-3 and reduced Bcl-2 expression. It was concluded that mangiferin triggered the apoptotic pathways. It also decreased the viability of TPC-1 cells by suppressing the PCNA.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0212-x
  • Enhanced Incorporation of Gaseous CO 2 to Succinate by a Recombinant
           Escherichia coli W3110
    • Authors: Soohyun Park; Hyeonsoo Kim; Sukhyeong Cho; Gwangro You; Han Bin Oh; Jun Hee Han; Jinwon Lee
      Abstract: Carbon dioxide (CO2) emissions are related to global warming. However, CO2 can be used as an abundant and cheap carbon source for production of valuable chemicals using carbon capture and storage technology. Here, the genes related to carbon flux toward pyruvate biosynthesis in E. coli were deleted to enhance the incorporation of CO2 for succinate production. The codonoptimized carbonic anhydrase gene (SP(-)HCCA) derived from Hahella chejuensis KCTC 2396 and the phosphoenolpyruvate carboxylase gene (ppc) of E. coli W3110 were co-overexpressed to enhance carbon flux toward oxaloacetate synthesis in E. coli. Finally, we constructed SGJS134, which shows the highest production of succinate derived from CO2 compared with other strains. SGJS134 produced approximately 6.5 mM succinate from CO2 and yielded approximately 13.0 mM succinate per dry cell weight. These results may be useful for enhancing the incorporation of CO2 for succinate production in E. coli. Additionally, the metabolic engineering method used in this study will propose the potential of E. coli to convert CO2 to valuable chemicals.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0177-9
  • CFD-DEM Simulation of the Fluidized-bed Granulation of Food Powders
    • Authors: Ju-Eun Kim; Young Mi Chung
      Abstract: In this study, the granulation behavior of food powders was simulated by taking into account the changes in the rheological properties and water content of the powders. Several food powders were studied and classified into self-agglomerating or non-agglomerating powders depending on the presence of a glass transition temperature. Cricket powder, which is incapable of self-agglomeration, was mixed with dextrin powder to enable the granulation process. Cricket + dextrin powder was used as a simulation model, and the glass transition temperature of dextrin and a newly derived evaporation model were adopted in our simulation studies. Similar tendencies were observed in both the experimental and simulation results. Our granulation model can describe all of the following: nucleation, granulation based on the powder properties and changes in particle water content due to evaporation; these behaviors have not been modeled in this way previously.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0382-6
  • Development of a Computational Fluid Dynamics Model for Scaling-up Ambr
    • Authors: Xianhua Li; Kara Scott; William J. Kelly; Zuyi Huang
      Abstract: It is known that process scaling-up has always been a challenge in biopharmaceutical and food industry. In recent years, newly emerging microscale bioreactors like Ambr15 and Ambr250 have attracted significant attention for that they can provide high throughput and accelerate upstream process development. In this work, we developed the first multiphase Computational Fluid Dynamics (CFD) model for an in-depth characterization of Ambr bioreactor systems. A number of advanced computational methods, including Reynolds stress turbulence model, population balance model, multiple reference frame (MRF), sliding mesh (SM) and user defined functions (UDFs), were integrated for the first time to systematically study the gas-liquid mixing in Ambr250 bioreactor. We provided detailed comparison between MRF and SM method, demonstrated the limitation of MRF for predicting bubble distribution in asymmetric reactors. Characteristics of hydrodynamics, mass transfer, turbulent dissipation and bubble size distribution were predicted from our CFD models and validated by existing experimental data for a variety of operating conditions for both the Ambr15 and Ambr250 bioreactors. The predicted kLa value ranges are 0.18–7.90 h-1 and 2.15–11.52 h-1 for Ambr250 and Ambr15, respectively. This work thus provides a superior framework for the computational modeling of microscale stirred bioreactors.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0063-5
  • Theobromine, a Methylxanthine in Cocoa Bean, Stimulates Thermogenesis by
           Inducing White Fat Browning and Activating Brown Adipocytes
    • Authors: Myeong Hwan Jang; Nam Hyeon Kang; Sulagna Mukherjee; Jong Won Yun
      Abstract: Natural medicinal compounds to treat obesity have recently attracted a great deal of attention because of the serious side effects of synthetic anti-obesity drugs. Recent advances have been made to identify natural products showing thermogenic activity, which is responsible for energy expenditure in brown or brown-like (beige) adipocytes. Here, we explored the thermogenic effects of theobromine, one of the most abundant methylxanthines in cocoa, on 3T3-L1 white adipocytes and HIB1B brown adipocytes. Theobromine markedly increased the expression levels of brown-fat signature proteins (PGC-1α, PRDM16, and UCP1) and beige-specific genes (Cd137, Cidea, Cited1, Tbx1, and Tmen26) in 3T3-L1 white adipocytes and remarkably elevated the expression levels of brown fatspecific genes (Cidea, Lhx8, Ppargc1, Prdm16, Ucp1, and Zic1) in HIB1B brown adipocytes. Theobromine also reduced the expression of the key adipogenic transcription factors, C/EBPα and PPARγ, in white adipocytes, while enhancing their expression in HIB1B cells. In addition, theobromine regulated lipolytic events and fat oxidation by upregulating the expression of pACC, ATGL, pHSL, ACOX, and CPT1. Additional mechanistic study revealed that theobromine activates β3-AR and AMPK. In summary, our results provide evidence for the first time indicating that theobromine has a potential beneficial effect on browning of white adipocytes and improves lipid catabolic metabolism in both cultured white and brown adipocytes via β-adrenergic signaling and AMPK activation. Consumption of theobromine may be a feasible way to activate thermogenesis and improve systematic lipid metabolism to protect against obesity and other metabolic disorders.
      PubDate: 2018-12-29
      DOI: 10.1007/s12257-018-0434-y
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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