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BIOCHEMISTRY (243 journals)                  1 2 | Last

Showing 1 - 200 of 243 Journals sorted alphabetically
AAPS PharmSciTech     Hybrid Journal   (Followers: 7)
Acetic Acid Bacteria     Open Access   (Followers: 2)
ACS Central Science     Open Access   (Followers: 9)
ACS Chemical Biology     Full-text available via subscription   (Followers: 282)
ACS Chemical Neuroscience     Full-text available via subscription   (Followers: 20)
Acta Biochimica Polonica     Open Access  
Acta Crystallographica Section D : Biological Crystallography     Hybrid Journal   (Followers: 10)
Acta Crystallographica Section F: Structural Biology Communications     Hybrid Journal   (Followers: 9)
Advances and Applications in Bioinformatics and Chemistry     Open Access   (Followers: 10)
Advances in Biological Chemistry     Open Access   (Followers: 8)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 7)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 7)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 18)
African Journal of Biochemistry Research     Open Access   (Followers: 1)
African Journal of Chemical Education     Open Access   (Followers: 3)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
American Journal of Biochemistry     Open Access   (Followers: 9)
American Journal of Biochemistry and Biotechnology     Open Access   (Followers: 61)
American Journal of Biochemistry and Molecular Biology     Open Access   (Followers: 15)
American Journal of Polymer Science     Open Access   (Followers: 30)
Amino Acids     Hybrid Journal   (Followers: 8)
Analytical and Bioanalytical Chemistry Research     Open Access   (Followers: 1)
Analytical Biochemistry     Hybrid Journal   (Followers: 164)
Angiogenesis     Hybrid Journal   (Followers: 3)
Annals of Clinical Biochemistry     Hybrid Journal   (Followers: 8)
Annual Review of Biochemistry     Full-text available via subscription   (Followers: 54)
Annual Review of Chemical and Biomolecular Engineering     Full-text available via subscription   (Followers: 12)
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42)
Applied Biochemistry and Microbiology     Hybrid Journal   (Followers: 16)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 8)
Archives of Biochemistry and Biophysics     Hybrid Journal   (Followers: 20)
Archives of Insect Biochemistry and Physiology     Hybrid Journal  
Archives Of Physiology And Biochemistry     Hybrid Journal  
Asian Journal of Biochemistry     Open Access   (Followers: 1)
Bangladesh Journal of Medical Biochemistry     Open Access   (Followers: 3)
BBA Clinical     Open Access  
BBR : Biochemistry and Biotechnology Reports     Open Access   (Followers: 4)
Biocatalysis     Open Access  
Biochemical and Biophysical Research Communications     Hybrid Journal   (Followers: 24)
Biochemical and Molecular Medicine     Full-text available via subscription   (Followers: 5)
Biochemical Compounds     Open Access  
Biochemical Engineering Journal     Hybrid Journal   (Followers: 15)
Biochemical Genetics     Hybrid Journal   (Followers: 3)
Biochemical Journal     Full-text available via subscription   (Followers: 24)
Biochemical Pharmacology     Hybrid Journal   (Followers: 10)
Biochemical Society Transactions     Full-text available via subscription   (Followers: 4)
Biochemical Systematics and Ecology     Hybrid Journal   (Followers: 3)
Biochemistry     Full-text available via subscription   (Followers: 333)
Biochemistry & Pharmacology : Open Access     Open Access   (Followers: 3)
Biochemistry & Physiology : Open Access     Open Access  
Biochemistry (Moscow)     Hybrid Journal   (Followers: 4)
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology     Hybrid Journal   (Followers: 3)
Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry     Hybrid Journal   (Followers: 3)
Biochemistry and Biophysics Reports     Open Access  
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 15)
Biochemistry and Molecular Biology Education     Hybrid Journal   (Followers: 6)
Biochemistry and Molecular Biology of Fishes     Full-text available via subscription   (Followers: 1)
Biochemistry Research International     Open Access   (Followers: 6)
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids     Hybrid Journal   (Followers: 7)
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease     Hybrid Journal   (Followers: 14)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research     Hybrid Journal   (Followers: 10)
Biochimie     Hybrid Journal   (Followers: 7)
Biochimie Open     Open Access  
Bioconjugate Chemistry     Full-text available via subscription   (Followers: 30)
BioDrugs     Full-text available via subscription   (Followers: 7)
Bioelectrochemistry     Hybrid Journal   (Followers: 2)
Biofarmasi Journal of Natural Product Biochemistry     Open Access  
Biofuels     Hybrid Journal   (Followers: 11)
Biogeochemistry     Hybrid Journal   (Followers: 15)
BioInorganic Reaction Mechanisms     Hybrid Journal   (Followers: 1)
Biokemistri     Open Access  
Biological Chemistry     Partially Free   (Followers: 22)
Biomaterials Research     Open Access   (Followers: 4)
Biomedicines     Open Access   (Followers: 1)
BioMolecular Concepts     Hybrid Journal   (Followers: 2)
Bioscience, Biotechnology, and Biochemistry     Hybrid Journal   (Followers: 21)
Biosimilars     Open Access   (Followers: 1)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44)
Bitácora Digital     Open Access  
BMC Biochemistry     Open Access   (Followers: 14)
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access  
Carbohydrate Polymers     Hybrid Journal   (Followers: 8)
Cell Biochemistry and Biophysics     Hybrid Journal   (Followers: 6)
Cell Biochemistry and Function     Hybrid Journal   (Followers: 7)
Cell Chemical Biology     Hybrid Journal   (Followers: 1)
Cellular Physiology and Biochemistry     Open Access   (Followers: 3)
ChemBioChem     Hybrid Journal   (Followers: 8)
Chemical and Biological Technologies for Agriculture     Open Access  
Chemical Biology & Drug Design     Hybrid Journal   (Followers: 20)
Chemical Engineering Journal     Hybrid Journal   (Followers: 52)
Chemical Senses     Hybrid Journal   (Followers: 1)
Chemical Speciation and Bioavailability     Open Access   (Followers: 1)
Chemico-Biological Interactions     Hybrid Journal   (Followers: 3)
Chemistry & Biodiversity     Hybrid Journal   (Followers: 7)
Chemistry & Biology     Full-text available via subscription   (Followers: 32)
Chemistry and Ecology     Hybrid Journal  
ChemTexts     Hybrid Journal  
Clinica Chimica Acta     Hybrid Journal   (Followers: 33)
Clinical Biochemist Reviews     Full-text available via subscription   (Followers: 1)
Clinical Biochemistry     Hybrid Journal   (Followers: 18)
Clinical Chemistry     Full-text available via subscription   (Followers: 69)
Clinical Chemistry and Laboratory Medicine     Hybrid Journal   (Followers: 62)
Clinical Lipidology     Full-text available via subscription   (Followers: 1)
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology     Hybrid Journal   (Followers: 5)
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 1)
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology     Hybrid Journal   (Followers: 6)
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics     Hybrid Journal   (Followers: 2)
Comprehensive Biochemistry     Full-text available via subscription   (Followers: 1)
Computational Biology and Chemistry     Hybrid Journal   (Followers: 11)
Critical Reviews in Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 5)
Current Biochemical Engineering     Hybrid Journal  
Current Chemical Biology     Hybrid Journal   (Followers: 2)
Current Medicinal Chemistry     Hybrid Journal   (Followers: 15)
Current Opinion in Chemical Biology     Hybrid Journal   (Followers: 31)
Current Opinion in Lipidology     Hybrid Journal   (Followers: 6)
DNA Barcodes     Open Access  
Doklady Biochemistry and Biophysics     Hybrid Journal   (Followers: 1)
Doklady Chemistry     Hybrid Journal  
Egyptian Journal of Biochemistry and Molecular Biology     Full-text available via subscription  
FABICIB     Open Access  
FEBS Letters     Hybrid Journal   (Followers: 58)
FEBS Open Bio     Open Access   (Followers: 3)
Fish Physiology and Biochemistry     Hybrid Journal   (Followers: 3)
Food & Function     Full-text available via subscription   (Followers: 7)
Foundations of Modern Biochemistry     Full-text available via subscription  
Free Radicals and Antioxidants     Full-text available via subscription   (Followers: 4)
Frontiers in Molecular Biosciences     Open Access   (Followers: 2)
Frontiers in Natural Product Chemistry     Hybrid Journal  
Global Biogeochemical Cycles     Full-text available via subscription   (Followers: 18)
Green Chemistry     Full-text available via subscription   (Followers: 10)
Histochemistry and Cell Biology     Hybrid Journal   (Followers: 7)
Indian Journal of Biochemistry and Biophysics (IJBB)     Open Access   (Followers: 2)
Indian Journal of Clinical Biochemistry     Hybrid Journal   (Followers: 1)
Indonesian Biomedical Journal     Open Access  
Insect Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 3)
International Journal of Biochemistry & Cell Biology     Hybrid Journal   (Followers: 6)
International Journal of Biochemistry and Biophysics     Open Access   (Followers: 1)
International Journal of Biological Chemistry     Open Access   (Followers: 2)
International Journal of Biomedical Nanoscience and Nanotechnology     Hybrid Journal   (Followers: 6)
International Journal of Food Contamination     Open Access  
International Journal of Plant Physiology and Biochemistry     Open Access  
International Journal of Plant Research     Open Access   (Followers: 3)
International Journal of Secondary Metabolite     Open Access   (Followers: 1)
Invertebrate Immunity     Open Access   (Followers: 1)
JBIC Journal of Biological Inorganic Chemistry     Hybrid Journal   (Followers: 4)
Journal of Microbial & Biochemical Technology     Open Access   (Followers: 2)
Journal of Applied Biology & Biotechnology     Open Access   (Followers: 2)
Journal of Bioactive and Compatible Polymers     Hybrid Journal   (Followers: 3)
Journal of Biochemical and Biophysical Methods     Hybrid Journal   (Followers: 4)
Journal of Biochemistry     Hybrid Journal   (Followers: 41)
Journal of Biochemistry and Molecular Biology Research     Open Access  
Journal of Biological Chemistry     Full-text available via subscription   (Followers: 200)
Journal of Biomaterials Science, Polymer Edition     Hybrid Journal   (Followers: 9)
Journal of Carbohydrate Chemistry     Hybrid Journal   (Followers: 6)
Journal of Cellular Biochemistry     Hybrid Journal   (Followers: 4)
Journal of Chemical Biology     Hybrid Journal   (Followers: 3)
Journal of Chemical Neuroanatomy     Hybrid Journal  
Journal of Clinical Lipidology     Hybrid Journal   (Followers: 2)
Journal of Comparative Physiology B : Biochemical, Systemic, and Environmental Physiology     Hybrid Journal   (Followers: 5)
Journal of Drug Discovery and Therapeutics     Open Access  
Journal of Enzyme Inhibition and Medicinal Chemistry     Open Access   (Followers: 3)
Journal of Evolutionary Biochemistry and Physiology     Hybrid Journal   (Followers: 1)
Journal of Food and Drug Analysis     Open Access  
Journal of Forensic Toxicology and Pharmacology     Hybrid Journal   (Followers: 6)
Journal of Inborn Errors of Metabolism and Screening     Open Access  
Journal of Inorganic Biochemistry     Hybrid Journal   (Followers: 6)
Journal of Medical and Biomedical Sciences     Open Access  
Journal of Medical Biochemistry     Open Access   (Followers: 4)
Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
Journal of Molecular Biochemistry     Open Access   (Followers: 4)
Journal of Molecular Diagnostics     Hybrid Journal   (Followers: 5)
Journal of Neurochemistry     Hybrid Journal   (Followers: 4)
Journal of Nutritional Biochemistry     Hybrid Journal   (Followers: 7)
Journal of Pediatric Biochemistry     Hybrid Journal   (Followers: 1)
Journal of Peptide Science     Hybrid Journal   (Followers: 21)
Journal of Photochemistry and Photobiology B: Biology     Hybrid Journal   (Followers: 3)
Journal of Physiobiochemical Metabolism     Hybrid Journal   (Followers: 2)
Journal of Physiology and Biochemistry     Hybrid Journal   (Followers: 3)
Journal of Plant Biochemistry and Biotechnology     Hybrid Journal   (Followers: 4)
Journal of Steroid Biochemistry and Molecular Biology     Hybrid Journal   (Followers: 2)
Journal of Virology & Antiviral Research     Hybrid Journal   (Followers: 6)
Journal of Wood Chemistry and Technology     Hybrid Journal   (Followers: 10)
La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine     Hybrid Journal  
Lab on a Chip     Full-text available via subscription   (Followers: 37)
Laboratory Techniques in Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 4)
Marine Chemistry     Hybrid Journal   (Followers: 7)
Methods in Enzymology     Full-text available via subscription   (Followers: 12)
Molecular and Biochemical Parasitology     Hybrid Journal   (Followers: 2)
Molecular and Cellular Biochemistry     Hybrid Journal   (Followers: 4)
Molecular Aspects of Medicine     Hybrid Journal   (Followers: 3)
Molecular Informatics     Hybrid Journal   (Followers: 5)
Molecular inhibitors in targeted therapy     Open Access  
Moscow University Chemistry Bulletin     Hybrid Journal   (Followers: 1)
Mycologia     Hybrid Journal  
Mycology : An International Journal on Fungal Biology     Hybrid Journal   (Followers: 5)
Natural Products and Bioprospecting     Open Access   (Followers: 2)
Nature Chemical Biology     Full-text available via subscription   (Followers: 74)
Nature Communications     Open Access   (Followers: 207)
Neurosignals     Open Access  
New Comprehensive Biochemistry     Full-text available via subscription  

        1 2 | Last

Journal Cover Archives of Biochemistry and Biophysics
  [SJR: 1.478]   [H-I: 138]   [20 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0003-9861 - ISSN (Online) 1096-0384
   Published by Elsevier Homepage  [3175 journals]
  • Bioavailability and pharmacokinetic profile of grape pomace phenolic
           compounds in humans
    • Authors: Fabio Castello; Giuseppina Costabile; Letizia Bresciani; Michele Tassotti; Daniele Naviglio; Delia Luongo; Paola Ciciola; Marilena Vitale; Claudia Vetrani; Gianni Galaverna; Furio Brighenti; Rosalba Giacco; Daniele Del Rio; Pedro Mena
      Pages: 1 - 9
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Fabio Castello, Giuseppina Costabile, Letizia Bresciani, Michele Tassotti, Daniele Naviglio, Delia Luongo, Paola Ciciola, Marilena Vitale, Claudia Vetrani, Gianni Galaverna, Furio Brighenti, Rosalba Giacco, Daniele Del Rio, Pedro Mena
      Grape pomace, the major byproduct of the wine and juice industry, is a relevant source of bioactive phenolic compounds. However, polyphenol bioavailability in humans is not well understood, and the inter-individual variability in the production of phenolic metabolites has not been comprehensively assessed to date. The pharmacokinetic and excretive profiles of phenolic metabolites after the acute administration of a drink made from red grape pomace was here investigated in ten volunteers. A total of 35 and 28 phenolic metabolites were quantified in urine and plasma, respectively. The main circulating metabolites included phenyl-γ-valerolactones, hydroxybenzoic acids, simple phenols, hydroxyphenylpropionic acids, hydroxycinnamates, and (epi)catechin phase II conjugates. A high inter-individual variability was shown both in urine and plasma samples, and different patterns of circulating metabolites were unravelled by applying unsupervised multivariate analysis. Besides the huge variability in the production of microbial metabolites of colonic origin, an important variability was observed due to phase II conjugates. These results are of interest to further understand the potential health benefits of phenolic metabolites on individual basis.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Tubeimoside V sensitizes human triple negative breast cancer
           MDA-MB-231 cells to anoikis via regulating caveolin-1-related signaling
    • Authors: Ke Wang; Xue Zhu; Yu Chen; Yongxiang Yin; Tao Ma
      Pages: 10 - 15
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Ke Wang, Xue Zhu, Yu Chen, Yongxiang Yin, Tao Ma
      Metastatic triple-negative breast cancer (TNBC) has poor outcome with conventional chemotherapy regimens due to its aggressive behavior. The acquisition of anoikis resistance, a programmed cell death process triggered by substratum detachment, is an important mechanism in TNBC metastasis. Therefore, agents that can restore the sensitivity of cancer cells to anoikis may be helpful for the treatment of metastatic TNBC. In this study, we investigated the inhibitory effect of Tubeimosides V (TBMS-V), a cyclic bisdesmoside isolated from the ethanol extracts of tubers of B. paniculatum., on anoikis resistance and the involvement of caveolin-1(CAV-1)-related signaling pathways in such process in MDA-MB-231 cells. The results showed that the treatment of TBMS-V could sensitize cancer cells to anoikis, which was associated with suppression of anchorage-independent culture-induced CAV-1 overexpression, EGFR activation as well as ITGB1-FAK activation. The data from this study might contribute to providing a potential therapeutic target for metastatic TNBC and suggest the possibility of TBMS-V and its derivatives for metastatic TNBC therapy.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Inhibition of anthrax lethal factor by ssDNA aptamers
    • Authors: Mieke Lahousse; Hae-Chul Park; Sang-Choon Lee; Na-Reum Ha; In-Pil Jung; Sara R. Schlesinger; Kaylin Shackelford; Moon-Young Yoon; Sung-Kun Kim
      Pages: 16 - 23
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Mieke Lahousse, Hae-Chul Park, Sang-Choon Lee, Na-Reum Ha, In-Pil Jung, Sara R. Schlesinger, Kaylin Shackelford, Moon-Young Yoon, Sung-Kun Kim
      Anthrax is caused by Bacillus anthracis, a bacterium that is able to secrete the toxins protective antigen, edema factor and lethal factor. Due to the high level of secretion from the bacteria and its severe virulence, lethal factor (LF) has been sought as a biomarker for detecting bacterial infection and as an effective target to neutralize toxicity. In this study, we found three aptamers, and binding affinity was determined by fluorescently labeled aptamers. One of the aptamers exhibited high affinity, with a K d value of 11.0 ± 2.7 nM, along with low cross reactivity relative to bovine serum albumin and protective antigen. The therapeutic functionality of the aptamer was examined by assessing the inhibition of LF protease activity against a mitogen-activated protein kinase kinase. The aptamer appears to be an effective inhibitor of LF with an IC50 value of 15 ± 1.5 μM and approximately 85% cell viability, suggesting that this aptamer provides a potential clue for not only development of a sensitive diagnostic device of B. anthracis infection but also the design of novel inhibitors of LF.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Cholesterol modulates the binding properties of human relaxin family
           peptide receptor 3 with its ligands
    • Authors: Jia-Hui Wang; Meng-Jun Hu; Xiao-Xia Shao; Dian Wei; Ya-Li Liu; Zeng-Guang Xu; Zhan-Yun Guo
      Pages: 24 - 30
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Jia-Hui Wang, Meng-Jun Hu, Xiao-Xia Shao, Dian Wei, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo
      Relaxin family peptide receptor 3 (RXFP3) is implicated in the regulation of food intake and stress response upon activation by its cognate agonist relaxin-3. As an A-class G protein-coupled receptor, RXFP3 is an integral plasma membrane protein with seven transmembrane domains, yet influence of the membrane lipids on its function remains unknown. In the present study, we disclosed that cholesterol, an essential membrane lipid for mammalian cells, modulated the binding properties of human RXFP3 with its ligands. We first demonstrated that depletion of cholesterol from host human embryonic kidney (HEK) 293T cells by methyl-β-cyclodextrin altered ligand-binding properties of the overexpressed human RXFP3, such as increasing its binding potency with some antagonists and decreasing its binding affinity with a NanoLuc-conjugated R3/I5 tracer. Thereafter, we demonstrated that two B-chain residues, B5Tyr and B12Arg, were primarily responsible for the increased binding potency of these antagonists with human RXFP3 under the cholesterol depletion condition. Our results suggest that cell membrane cholesterol interacts with human RXFP3 and modulates its ligand-binding properties, providing new insights into the influence of membrane lipids on RXFP3 function.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Biological and pharmacological effects of hexahydrocurcumin, a metabolite
           of curcumin
    • Authors: Yiyuan Huang; Shijie Cao; Qiang Zhang; Hongyang Zhang; Yuqi Fan; Feng Qiu; Ning Kang
      Pages: 31 - 37
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Yiyuan Huang, Shijie Cao, Qiang Zhang, Hongyang Zhang, Yuqi Fan, Feng Qiu, Ning Kang
      Curcumin, one of the most precious pharmacologically relevant natural products, has gained considerable attention among scientists for decades because of its multi-pharmacological activities in the clinical. However, critical studies on its pharmacological and toxicological activities are needed to understand how this compound can have these biological functions considering its poor oral bioavailability and the low plasma concentration. Moreover, curcumin undergoes extensive and rapid metabolism in vivo, indicating that the pharmacological activity of consuming curcumin might be mediated partly by its metabolites. And as one of the major curcumin metabolites, hexahydrocurcumin (HHC), exhibits similar or more potent bioactivity than curcumin by in vitro and in vivo studies, such as antioxidant, anti-inflammatory, antitumor and cardiovascular protective properties, which may provide important information for us to have a profound comprehension of the effectiveness of curcumin. This review mainly summarizes the current knowledge and underlying molecular mechanisms of the biological activities of HHC and its potential effects on the development of various human diseases.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • A mass spectrometry approach for the identification and localization of
           small aldehyde modifications of proteins
    • Authors: Catarina B. Afonso; Bebiana C. Sousa; Andrew R. Pitt; Corinne M. Spickett
      Pages: 38 - 45
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Catarina B. Afonso, Bebiana C. Sousa, Andrew R. Pitt, Corinne M. Spickett
      Lipids containing polyunsaturated fatty acids are primary targets of oxidation, which produces reactive short-chain aldehydes that can covalently modify proteins, a process called lipoxidation. Improved mass spectrometry (MS) methods for the analysis of these adducts in complex biological systems are needed. Lysozyme and human serum albumin (HSA) were used as model proteins to investigate lipoxidation products formed by two short-chain aldehydes, acrolein and pentanal, which are unsaturated and saturated aldehydes respectively. The adducts formed were stabilized by NaBH4 or NaBH3CN reduction and analysed by MS. Analysis of intact modified lysozyme showed a pentanal modification resulting from Schiff's base formation (+70 Da), and up to 8 acrolein adducts, all resulting from Michael addition (+58 Da). Analysis of tryptic digests identified specific histidine, cysteine and lysine residues modified in both lysozyme and HSA, and determined characteristic amino acid-specific fragmentations. Eight different internal fragment ions were found that could be used as general diagnostic ions for pentanal- and acrolein-modified amino acids. The combined use of intact protein analysis and LC-MS/MS methods provided a powerful tool for the identification and localization of aldehyde-protein adducts, and the diagnostic ions will facilitate the development of targeted MS methods for analysis of adducts in more complex samples.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Optical assessment of skin carotenoid status as a biomarker of vegetable
           and fruit intake
    • Authors: Igor V. Ermakov; Maia Ermakova; Mohsen Sharifzadeh; Aruna Gorusupudi; Kelliann Farnsworth; Paul S. Bernstein; Jodi Stookey; Jane Evans; Tito Arana; Lisa Tao-Lew; Carly Isman; Anna Clayton; Akira Obana; Leah Whigham; Alisha H. Redelfs; Lisa Jahns; Werner Gellermann
      Pages: 46 - 54
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Igor V. Ermakov, Maia Ermakova, Mohsen Sharifzadeh, Aruna Gorusupudi, Kelliann Farnsworth, Paul S. Bernstein, Jodi Stookey, Jane Evans, Tito Arana, Lisa Tao-Lew, Carly Isman, Anna Clayton, Akira Obana, Leah Whigham, Alisha H. Redelfs, Lisa Jahns, Werner Gellermann
      Resonance Raman spectroscopy (RRS) and reflection spectroscopy (RS) are optical methods applicable to the non-invasive detection of carotenoids in human skin. RRS is the older, more thoroughly validated method, whereas RS is newer and has several advantages. Since collective skin carotenoid levels serve as a biomarker for vegetable and fruit intake, both methods hold promise as convenient screening tools for assessment of dietary interventions and correlations between skin carotenoids and health and disease outcomes. In this manuscript, we describe the most recent optimized device configurations and compare their use in various clinical and field settings. Both RRS and RS devices yield a wide range of skin carotenoid levels between subjects, which is a critical feature for a biomarker. Repeatability of the methods is 3–15% depending on the subject's skin carotenoid level and the uniformity of its local distribution. For 54 subjects recruited from an ophthalmology clinic, we first checked the validity of the relatively novel RS methodology via biochemical serum carotenoid measurements, the latter carried out with high performance liquid chromatography (HPLC). A high correlation between RS skin and serum HPLC carotenoid levels was established (R = 0.81; p < 0.001). Also, a high correlation was found between RS and RRS skin levels (R = 0.94 p < 0.001). Subsequent comparisons of skin carotenoid measurements in diverse age groups and ethnicities included 569 Japanese adults, 947 children with ages 2–5 screened in 24 day care centers in San Francisco, and 49 predominantly Hispanic adults screened at an outdoor health fair event. Depending on the particular subject group, correlation coefficients between the RRS and RS methods ranged between R ∼0.80 and R ∼0.96. Analysis of the Japanese screening showed that, on average, skin carotenoid levels are higher in women compared to men, skin levels do not depend on age, and tobacco smokers have reduced levels versus non-smokers. For the two most ethnically diverse groups with widely varying melanin levels, we investigated the effect of dermal melanin on RS and RRS skin carotenoid levels. The analysis revealed that large variations in skin carotenoid levels remain detectable independent of the particular melanin index. This behavior is consistent with the absence of melanin effects on the skin carotenoid levels generated with the instrument configurations. The RS method has an advantage over RRS in its relative simplicity. Due to its detection of skin reflection over a wide spectral range from the near UV to the near IR, it has the unique ability to quantify each of the major tissue chromophores and take them into account in the derivation of skin carotenoid levels.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • (-)-Epicatechin and its metabolites prevent palmitate-induced NADPH
           oxidase upregulation, oxidative stress and insulin resistance in HepG2
    • Authors: Eleonora Cremonini; Patricia I. Oteiza
      Pages: 55 - 63
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Eleonora Cremonini, Patricia I. Oteiza
      While diets rich in fruit and vegetables can decrease the risk for type 2 diabetes (T2D), diets rich in fat and carbohydrates can increase it. The flavanol-3-ol (-)-epicatechin (EC) can improve insulin sensitivity both in humans and animal models of T2D. NADPH oxidases and oxidative stress can contribute to the development of insulin resistance. This study investigated the capacity of EC and EC metabolites (ECM) to downregulate NADPH oxidases and oxidative stress, and its association to an improvement of insulin sensitivity. This was studied in in vivo (high fat-fed mice) and in vitro (HepG2 cells) conditions of hepatic lipid overload. EC decreased NOX3/NOX4 liver expression and mitigated oxidative stress in high fat-fed mice. In HepG2 cells, incubation with palmitate increased: i) lipid deposition, ii) NOX3/NOX4 expression, iii) NADPH oxidase activity, and iv) oxidative stress; promoting v) the activation of redox-sensitive kinases (JNK and IKK), and vi) impaired insulin responses. Physiological concentrations of EC and ECM, and NADPH oxidase inhibitors (apocynin, VAS2870) prevented all those deleterious effects of palmitate. The obtained results points to NADPH oxidases as an important target in the capacity of EC to improve insulin sensitivity in conditions of liver lipid overload, as those associated with Western-style diets.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Undesirable impact on structure and stability of insulin on addition of
           (+)-catechin hydrate with sugar
    • Authors: Anjeeta Rani; Indrani Jha; Pannuru Venkatesu
      Pages: 64 - 71
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Anjeeta Rani, Indrani Jha, Pannuru Venkatesu
      Insulin (In) based formulation has been used over decades for the cure of In-dependent diabetic patients, however, more attempts are still required to improve the remedial use of In. In this regard, the use of green tea has become widespread nowadays. However, it is unknown that (+)-catechin hydrate (CAT), a major component of green tea which enhances anti-diabetic activity of In, will or will not enhance the structure and stability of In if ingested with sugars. Interestingly, by using biophysical techniques, present study reveals the fact that the use of sugar during the intake of green tea extract may produce unwanted effects on In which may further lead to some disorders associated with In stability and also create obstacle in successful implications of In formulations.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Histone acetyltransferase 1 up regulates Bcl2L12 expression in
           nasopharyngeal cancer cells
    • Authors: Bei-Ping Miao; Rui-Shi Zhang; Gui Yang; Jin-Jie Sun; Yu-Yan Tang; Wei-Feng Liang; Tao Liu; Zhong Wen; Ping-Chang Yang; Guo-Hui Nie
      Pages: 72 - 79
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Bei-Ping Miao, Rui-Shi Zhang, Gui Yang, Jin-Jie Sun, Yu-Yan Tang, Wei-Feng Liang, Tao Liu, Zhong Wen, Ping-Chang Yang, Guo-Hui Nie
      The deregulation of Bcl2L12 expression in cancer has been recognized, but the causative factors are unknown. Histone acetyltransferases (HAT) play critical roles in the regulation gene transcription. This study tests a hypothesis that the aberrant activities of HAT induce deregulation of Bcl2L12 in nasopharyngeal cancer (NPC). In this study, human NPC tissues were collected from the clinic. The expression of Bcl2L12 and HATs in NPC cells was analyzed by real time RT-PCR and Western blotting. NPC cell apoptosis was analyzed by flow cytometry. The results showed that by screening the subtypes of HAT, the levels of HAT1 were uniquely higher in NPC as compared with non-cancer nasopharyngeal tissue. The levels of Bcl2L12 in NPC cells were positively correlated with HAT1. HAT1 involved in the STAT5 binding to the Bcl2L12 promoter. HAT1 increased the expression of Bcl2L12. Bcl2L12 mediated the effects of HAT1 on suppressing NPC cell apoptosis. Absorption of the HAT1 shRNA plasmid-carrying liposomes induced NPC cell apoptosis. In conclusion, inhibition of HAT1 can induce NPC cell apoptosis via increasing Bcl2L12 expression, which can be a potential therapy for NPC treatment.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Interactions of staphyloxanthin and enterobactin with myeloperoxidase and
           reactive chlorine species
    • Authors: Melanie S. Coker; Louisa V. Forbes; Matthew Plowman-Holmes; David R. Murdoch; Christine C. Winterbourn; Anthony J. Kettle
      Pages: 80 - 89
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Melanie S. Coker, Louisa V. Forbes, Matthew Plowman-Holmes, David R. Murdoch, Christine C. Winterbourn, Anthony J. Kettle
      When neutrophils engulf bacteria, myeloperoxidase converts hydrogen peroxide to hypochlorous acid, which is toxic to all micro-organisms. It has been suggested that some pathogens have virulence factors that target myeloperoxidase to dampen the oxidative reactions of neutrophils. These virulence factors include staphyloxanthin, the golden pigment of Staphylococcus aureus, and enterobactin – a siderophore released by gram-negative bacteria. We investigated the potential of staphyloxanthin and enterobactin to shield bacteria from hypochlorous acid and related chloramines. Clinical strains of S. aureus with high levels of staphyloxanthin and related carotenoids were in general more resistant to low doses of hypochlorous acid than non-pigmented bacteria. But some non-pigmented strains were also resistant to the oxidant. Doses of reactive chlorine species that killed bacteria also bleached their carotenoids. Hypochlorous acid, NH2Cl, and NHCl2 bleached purified staphyloxanthin. When S. aureus were phagocytosed by neutrophils there was no discernible loss of staphyloxanthin. These data suggest that staphyloxanthin is capable of protecting bacteria from low doses of reactive chlorine species formed inside phagosomes. Enterobactin was not an inhibitor of myeloperoxidase. We conclude that staphyloxanthin may protect some bacterial strains against oxidative killing by neutrophils, but enterobactin will not inhibit the production of hypochlorous acid.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Metabolic changes in urine and serum during progression of diabetic kidney
           disease in a mouse model
    • Authors: Nan Hee Kim; Jin Seong Hyeon; Nam Hoon Kim; Ahreum Cho; Gayoung Lee; Seo Young Jang; Mi-Kyung Kim; Eun Young Lee; Choon Hee Chung; Hunjoo Ha; Geum Sook Hwang
      Pages: 90 - 97
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Nan Hee Kim, Jin Seong Hyeon, Nam Hoon Kim, Ahreum Cho, Gayoung Lee, Seo Young Jang, Mi-Kyung Kim, Eun Young Lee, Choon Hee Chung, Hunjoo Ha, Geum Sook Hwang
      Diabetic kidney disease (DKD) involves various pathogenic processes during progression to end stage renal disease, and activated metabolic pathways might be changing based on major pathophysiologic mechanisms as DKD progresses. In this study, nuclear magnetic resonance spectroscopy (NMR)-based metabolic profiling was performed in db/db mice to suggest potential biomarkers for early detection and its progression. We compared concentrations of serum and urinary metabolites between db/m and db/db mice at 8 or 20 weeks of age and investigated whether changes between 8 and 20 weeks in each group were significant. The metabolic profiles demonstrated significantly increased urine levels of glucose and tricarboxylic acid cycle intermediates at both 8 and 20 weeks of age in db/db mice. These intermediates also exhibited strong positive associations with urinary albumin excretion, suggesting that they may be potential biomarkers for early diagnosis. On the contrary, branched chain amino acid and homocysteine-methionine metabolism were activated early in the disease, whereas ketone and fatty acid metabolism were significantly changed in the late phase of the disease. We demonstrated phase-specific alterations in metabolites during progression of DKD. This study provides insights into perturbed mechanisms during evolution of the disease and identifies potential novel biomarkers for DKD.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Theophylline suppresses interleukin-6 expression by inhibiting
           glucocorticoid receptor signaling in pre-adipocytes
    • Authors: Takakazu Mitani; Tomohide Takaya; Naoki Harada; Shigeru Katayama; Ryoichi Yamaji; Soichiro Nakamura; Hitoshi Ashida
      Pages: 98 - 106
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Takakazu Mitani, Tomohide Takaya, Naoki Harada, Shigeru Katayama, Ryoichi Yamaji, Soichiro Nakamura, Hitoshi Ashida
      Adipose tissues in obese individuals are characterized by a state of chronic low-grade inflammation. Pre-adipocytes and adipocytes in this state secrete pro-inflammatory adipokines, such as interleukin 6 (IL-6), which induce insulin resistance and hyperglycemia. Theophylline (1,3-dimethylxanthine) exerts anti-inflammatory effects, but its effects on pro-inflammatory adipokine secretion by pre-adipocytes and adipocytes have not been examined. In this study, we found that theophylline decreased IL-6 secretion by 3T3-L1 pre-adipocytes and mouse-derived primary pre-adipocytes. The synthetic glucocorticoid dexamethasone (DEX) induced IL-6 expression in 3T3-L1 pre-adipocytes, and this effect was suppressed by theophylline at the mRNA level. Knockdown of CCAAT/enhancer binding protein (C/EBP) δ inhibited DEX-induced IL-6 expression, and theophylline suppressed C/EBPδ expression. Furthermore, theophylline suppressed transcriptional activity of the glucocorticoid receptor (GR) through suppression of nuclear localization of GR. In vivo, glucocorticoid corticosterone treatment (100 μg/mL) increased fasting blood glucose and plasma IL-6 levels in C57BL/6 N mice. Theophylline administration (0.1% diet) reduced corticosterone-increased fasting blood glucose, plasma IL-6 levels, and Il6 gene expression in adipose tissues. These results show that theophylline administration attenuated glucocorticoid-induced hyperglycemia and IL-6 production by inhibiting GR activity. The present findings indicate the potential of theophylline as a candidate therapeutic agent to treat insulin resistance and hyperglycemia.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Research trends in flavonoids and health
    • Authors: Francisco Perez-Vizcaino; Cesar G. Fraga
      Pages: 107 - 112
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Francisco Perez-Vizcaino, Cesar G. Fraga
      Herein we describe, based on some bibliometric data, how the field of research on flavonoids has evolved over the last 25 years. The number of papers on flavonoids has risen in an exponential manner over these years, much faster than other fields on food constituents. This increase appears to be related to the contemporary explosion of interest in healthy foods, supplements and nutraceuticals. It was also probably triggered by large epidemiological studies on fruits and vegetables, and particularly on flavonoids, consumption and incidence of cancer, stroke and coronary heart disease. The widely distributed flavonols constitute the flavonoid subgroup upon which the greatest interest has been focused, followed by flavanols and more recently by anthocyanidins and other related polyphenols such as resveratrol. Research on isoflavones rapidly emerged in the 1990s but plateaued in the 2000s. In the 1990s flavonoids were mainly considered as the active components of medicinal plants, while from 2000 onward, they switched to be mainly regarded as bioactive food ingredients. We envision a continuation in the growth of research for the coming decade focused on clearly demonstrating the importance of flavonoids for human health.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Long noncoding RNA BDNF-AS is downregulated in cervical cancer and has
           anti-cancer functions by negatively associating with BDNF
    • Authors: Huimin Zhang; Caihong Liu; Ting Yan; Jun Wang; Wentong Liang
      Pages: 113 - 119
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Huimin Zhang, Caihong Liu, Ting Yan, Jun Wang, Wentong Liang
      Purpose We investigated expression and mechanism long noncoding RNA BDNF-AS in human cervical cancer (CC). Methods BDNF-AS expressions were examined by qPCR in CC cell lines and human CC tumors. CC cell lines, SiHa and DoTc2-4510 were transduced with lentivirus to ectopically overexpress BDNF-AS. Possible anti-cancer effects of BDNF overexpression were examined on CC in vitro proliferation and migration, and in vivo transplantation. Human BDNF gene expression was also examined in CC cell lines and tumors. In CC cells with overexpressed BDNF-AS, BDNF was upregulated to examine its direct effect in NDNF-AS-modulated CC proliferation and migration. Results BDNF was downregulated in both CC cells and human CC tumors. In CC cells, BDNF-AS overexpression is anti-cancer by inhibiting proliferation and migration in vitro, and transplantation in vivo. BDNF was inversely expressed as BDNF-AS in CC. Upregulation of BDNF in BDNF-AS-overexpressed CC cells reversed the anti-cancer effects of BDNF-AS. Conclusion BDNF-AS is downregulated in CC. Overexpressing BDNF-AS may inhibit CC, possibly through inverse regulation on BDNF.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Characterisation of peroxidasin activity in isolated extracellular matrix
           and direct detection of hypobromous acid formation
    • Authors: Boushra Bathish; Rufus Turner; Martina Paumann-Page; Anthony J. Kettle; Christine C. Winterbourn
      Pages: 120 - 127
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Boushra Bathish, Rufus Turner, Martina Paumann-Page, Anthony J. Kettle, Christine C. Winterbourn
      Peroxidasin is a heme peroxidase that catalyses the oxidation of bromide by hydrogen peroxide to form an essential sulfilimine cross-link between methionine and hydroxylysine residues in collagen IV. We investigated cross-linking by peroxidasin embedded in extracellular matrix isolated from cultured epithelial cells and its sensitivity to alternative substrates and peroxidase inhibitors. Peroxidasin showed peroxidase activity as measured with hydrogen peroxide and Amplex red. Using a specific mass spectrometry assay that measures NADH bromohydrin, we showed definitively that the enzyme releases hypobromous acid (HOBr). Less than 1 μM of the added hydrogen peroxide was used by peroxidasin. The remainder was consumed by catalase activity that was associated with the matrix. Results from NADH bromohydrin measurements indicates that low micromolar HOBr generated by peroxidasin was sufficient for maximum sulfilimine cross-linking, whereas 100 μM reagent HOBr or taurine bromamine was less efficient. This implies selectivity for the enzymatic process. Physiological concentrations of thiocyanate and urate partially inhibited cross-link formation. 4-Aminobenzoic acid hydrazide, a commonly used myeloperoxidase inhibitor, also inhibited peroxidasin, whereas acetaminophen and a 2-thioxanthine were much less effective. In conclusion, HOBr is produced by peroxidasin in the extracellular matrix. It appears to be directed at the site of collagen IV sulfilimine formation but the released HOBr may also undergo other reactions.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Long noncoding RNA SNHG6 promotes osteosarcoma cell proliferation through
           regulating p21 and KLF2
    • Authors: Jianwei Ruan; Longlong Zheng; Nan Hu; Guoyi Guan; Jialin Chen; Xiaozhong Zhou; Maoquan Li
      Pages: 128 - 136
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Jianwei Ruan, Longlong Zheng, Nan Hu, Guoyi Guan, Jialin Chen, Xiaozhong Zhou, Maoquan Li
      The effects of long non-coding RNAs (lncRNAs) on cellular biological processes and even the tumorigenesis have been widely reported. Small nucleolar RNA host gene 6 (SNHG6) has been reported to participate in regulating biological behaviors of multiple types of cancers. Nevertheless, the functions of SNHG6 in osteosarcoma still remain to be uncovered. This study intended to determine the clinical significance and biological functions of SNHG6 in osteosarcoma. It was confirmed by qRT-PCR that SNHG6 was highly expressed in osteosarcoma tissues and cell lines. Highly expressed SNHG6 predicted poor survival rate and advanced clinical stage for osteosarcoma patients, according to Kaplan-Meier method and Cox regression analysis. Loss-of-function assays were performed to examine the effects of silenced SNHG6 on the progression of osteosarcoma, indicating that silenced SNHG6 suppressed cell proliferation through inducing cell cycle arrest in G0/G1 phase and causing cell apoptosis. In vitro assays exposed the potential oncogenic role of SNHG6 in osteosarcoma, further affirmed by in vivo nude mice assays. Mechanistic assays demonstrated that SNHG6 was negatively correlated with p21 and KLF2 in osteosarcoma. And biological functions of SNHG6 in osteosarcoma were realized through regulating p21 and KLF2. Collectively, SNHG6 was a new type of molecule involving in the progression of osteosarcoma.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Excited-state dynamics of 3,3′-dihydroxyisorenieratene and
           (3R,3′R)-zeaxanthin: Observation of vibrationally hot S0 species
    • Authors: Florian Ehlers; Mirko Scholz; Kawon Oum; Thomas Lenzer
      Pages: 137 - 144
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Florian Ehlers, Mirko Scholz, Kawon Oum, Thomas Lenzer
      We report on an ultrafast transient absorption study of all-trans-3,3′-dihydroxyisorenieratene (“DHIR”) and all-trans-(3R,3′R)-zeaxanthin in organic solvents covering the wavelength range 350–770 nm. The lifetime of the S2 state in both carotenoids is 160–170 fs. Upon internal conversion (IC) non-equilibrated S1 molecules are formed which internally relax on a 300–400 fs time scale. The time constant for IC from S1 depends on the type of terminal substituent: Replacement of the two terminal β-ionone rings of zeaxanthin by two aryl rings in DHIR results in an increase from 9.5 to 10.9 ps in THF. This suggests a mild decrease in the effective conjugation length of DHIR. IC to the ground electronic state prepares vibrationally hot S0* molecules which exhibit characteristic bleach and absorption bands. These are typically denoted as “S* features”. Collisional cooling of S0* happens with a time constant of 15 ps. Based on our results and the findings from previous studies for other carotenoids, such as macro-β-carotenes, β-carotenes and long-chain apocarotenals, we conclude that S0* spectral features are ubiquitous in carotenoid photophysics: They are particularly easy to observe in systems with a very short S1 lifetime and a high quantum yield for IC to the ground electronic state.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Effects of grape consumption on biomarkers of inflammation, endothelial
           function, and PBMC gene expression in obese subjects
    • Authors: Allison S. Bardagjy; Qian Hu; Kathryn A. Giebler; Addison Ford; Francene M. Steinberg
      Pages: 145 - 152
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Allison S. Bardagjy, Qian Hu, Kathryn A. Giebler, Addison Ford, Francene M. Steinberg
      This study investigated effects of grape consumption on biomarkers of cardiovascular health in obese participants in both postprandial and chronic settings. Twenty obese adults participated in this randomized, placebo controlled, double-blinded crossover trial. Participants were randomized to consume 60 g freeze-dried polyphenol-rich whole grape powder (GP) or placebo (PBO) followed by high fat high carbohydrate (HFHC) meal challenge. Following acute challenge, participants consumed their respective treatment daily for 4 weeks to determine effects of chronic consumption. Consumption of GP with HFHC meal significantly increased nuclear factor (erythroid-derived 2)-like 2 (NRF2) expression in peripheral blood mononuclear cells (PBMC) at 3 h (p < 0.05) and decreased plasma endothelin-1 (ET-1) concentration at 5 h (p < 0.05) after meal challenge compared with PBO. Following 4 weeks of daily GP consumption, soluble vascular cell adhesion molecule 1 (sVCAM-1) plasma concentration increased compared with PBO (p < 0.05), however baseline values differed between treatments. In conclusion, GP consumption resulted in decreased vasoconstrictor ET-1 concentration and increased gene expression related to oxidative stress defense following HFHC meal. Except for increase in sVCAM-1 concentration, 4 weeks of chronic GP consumption had little effect on cardiovascular biomarkers measured in this study. This trial was registered: NCT01674231.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Plasma proteins associated with circulating carotenoids in Nepalese
           school-aged children
    • Authors: Abdulkerim Eroglu; Kerry J. Schulze; James Yager; Robert N. Cole; Parul Christian; Bareng A.S. Nonyane; Sun Eun Lee; Lee S.F. Wu; Subarna Khatry; John Groopman; Keith P. West
      Pages: 153 - 160
      Abstract: Publication date: 15 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 646
      Author(s): Abdulkerim Eroglu, Kerry J. Schulze, James Yager, Robert N. Cole, Parul Christian, Bareng A.S. Nonyane, Sun Eun Lee, Lee S.F. Wu, Subarna Khatry, John Groopman, Keith P. West
      Carotenoids are naturally occurring pigments that function as vitamin A precursors, antioxidants, anti-inflammatory agents or biomarkers of recent vegetable and fruit intake, and are thus important for population health and nutritional assessment. An assay approach that measures proteins could be more technologically feasible than chromatography, thus enabling more frequent carotenoid status assessment. We explored associations between proteomic biomarkers and concentrations of 6 common dietary carotenoids (α-carotene, β-carotene, lutein/zeaxanthin, β-cryptoxanthin, and lycopene) in plasma from 500 6–8 year old Nepalese children. Samples were depleted of 6 high-abundance proteins. Plasma proteins were quantified using tandem mass spectrometry and expressed as relative abundance. Linear mixed effects models were used to determine the carotenoid:protein associations, accepting a false discovery rate of q < 0.10. We quantified 982 plasma proteins in >10% of all child samples. Among these, relative abundance of 4 were associated with β-carotene, 11 with lutein/zeaxanthin and 51 with β-cryptoxanthin. Carotenoid-associated proteins are notably involved in lipid and vitamin A transport, antioxidant function and anti-inflammatory processes. No protein biomarkers met criteria for association with α-carotene or lycopene. Plasma proteomics may offer an approach to assess functional biomarkers of carotenoid status, intake and biological function for public health application. Original maternal micronutrient trial from which data were derived as a follow-up activity was registered at NCT00115271.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 646 (2018)
  • Identification and structural characterization of a novel myeloperoxidase
           inhibitor from Staphylococcus delphini
    • Authors: Nicoleta T. Ploscariu; Nienke W.M. de Jong; Kok P.M. van Kessel; Jos A.G. van Strijp; Brian V. Geisbrecht
      Pages: 1 - 11
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Nicoleta T. Ploscariu, Nienke W.M. de Jong, Kok P.M. van Kessel, Jos A.G. van Strijp, Brian V. Geisbrecht
      Staphylococcus aureus and related species are highly adapted to their hosts and have evolved numerous strategies to evade the immune system. S. aureus shows resistance to killing following uptake into the phagosome, which suggests that the bacterium evades intracellular killing mechanisms used by neutrophils. We recently discovered an S. aureus protein (SPIN for Staphylococcal Peroxidase INhibitor) that binds to and inhibits myeloperoxidase (MPO), a major player in the oxidative defense of neutrophils. To allow for comparative studies between multiple SPIN sequences, we identified a panel of homologs from species closely related to S. aureus. Characterization of these proteins revealed that SPIN molecules from S. agnetis, S. delphini, S. schleiferi, and S. intermedius all bind human MPO with nanomolar affinities, and that those from S. delphini, S. schleiferi, and S. intermedius inhibit human MPO in a dose-dependent manner. A 2.4 Å resolution co-crystal structure of SPIN-delphini bound to recombinant human MPO allowed us to identify conserved structural features of SPIN proteins, and to propose sequence-dependent physical explanations for why SPIN-aureus binds human MPO with higher affinity than SPIN-delphini. Together, these studies expand our understanding of MPO binding and inhibition by a recently identified component of the staphylococcal innate immune evasion arsenal.
      Graphical abstract image

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Exercise training protects against cancer-induced cardiac remodeling in an
           animal model of urothelial carcinoma
    • Authors: Ana Isabel Padrão; Rita Nogueira-Ferreira; Rui Vitorino; Dulce Carvalho; Catarina Correia; Maria João Neuparth; Maria João Pires; Ana Isabel Faustino-Rocha; Lúcio Lara Santos; Paula Alexandra Oliveira; José Alberto Duarte; Daniel Moreira-Gonçalves; Rita Ferreira
      Pages: 12 - 18
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Ana Isabel Padrão, Rita Nogueira-Ferreira, Rui Vitorino, Dulce Carvalho, Catarina Correia, Maria João Neuparth, Maria João Pires, Ana Isabel Faustino-Rocha, Lúcio Lara Santos, Paula Alexandra Oliveira, José Alberto Duarte, Daniel Moreira-Gonçalves, Rita Ferreira
      Limiting cancer-induced cardiac damage has become an increasingly important issue to improve survival rates and quality of life. Exercise training has been shown to reduce cardiovascular complications in several diseases; however, its therapeutic role against cardiovascular consequences of cancer is in its infancy. In order to add new insights on the potential therapeutic effect of exercise training on cancer-related cardiac dysfunction, we used an animal model of urothelial carcinoma submitted to 13 weeks of treadmill exercise after 20 weeks of exposure to the carcinogenic N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). Data showed that 13 weeks of treadmill exercise reverted cancer-induced cardiomyocytes atrophy and fibrosis, improved cardiac oxidative capacity given by citrate synthase activity and MnSOD content, and increased the levels of the mitochondrial biogenesis markers PGC-1α and mtTFA. Moreover, exercise training reverted cancer-induced decrease of cardiac c-kit levels suggesting enhanced regenerative ability of heart. These cardiac adaptations to exercise were related to a lower incidence of malignant urothelial lesions and less signs of inflammation. Taken together, data from the present study support the beneficial effect of exercise training when started after cancer diagnosis, envisioning the improvement of the cardiovascular function.
      Graphical abstract image

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Integrin-mediated human glioblastoma cells adhesion, migration and
           invasion by native and recombinant phospholipases of Scorpio maurus venom
    • Authors: Najeh Krayem; Zaineb Abdelkefi-Koubaa; Youssef Gargouri; José Luis
      Pages: 19 - 25
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Najeh Krayem, Zaineb Abdelkefi-Koubaa, Youssef Gargouri, José Luis
      Integrins are a large family of cell surface receptors mediating the interaction of cells with their microenvironment and they play an important role in glioma biology. In the present work, we reported the anti-tumor effect of Sm-PLGV a phospholipase A2 from Tunisian scorpion venom glands-as well as its recombinant forms expressed in Escherichia coli-through interference with integrin receptor function in malignant glioma cells U87. These phospholipases inhibited in a dose dependent manner the adhesion, migration and invasion onto fibrinogen and fibronectin without any cytotoxicity. We showed that Sm-PLGV and its recombinant constructs blocked U87 migration by reducing their velocity and directional persistence. The inhibitory effect was related to a blockage of the integrins αvβ3 and α5β1 function. Inactivation of the enzymatic activity of Sm-PLGV by chemical modification with p-bromophenacyl bromide did not affect its anti-tumor properties, suggesting the presence of ‘pharmacological sites’ distinct from the catalytic site in scorpion venom phospholipases A2.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Deuteration of human carbonic anhydrase for neutron crystallography: Cell
           culture media, protein thermostability, and crystallization behavior
    • Authors: K. Koruza; B. Lafumat; Á. Végvári; W. Knecht; S.Z. Fisher
      Pages: 26 - 33
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): K. Koruza, B. Lafumat, Á. Végvári, W. Knecht, S.Z. Fisher
      Deuterated proteins and other bio-derived molecules are important for NMR spectroscopy, neutron reflectometry, small angle neutron scattering, and neutron protein crystallography. In the current study we optimized expression media and cell culture conditions to produce high levels of 3 different deuterated human carbonic anhydrases (hCAs). The labeled hCAs were then characterized and tested for deuterium incorporation by mass spectrometry, temperature stability, and propensity to crystallize. The results show that is possible to get very good yields (>10 mg of pure protein per liter of cell culture under deuterated conditions) and that protein solubility is unaffected at the crystallization concentrations tested. Using unlabeled carbon source and recycled heavy water, we were able to get 65–77% deuterium incorporation, sufficient for most neutron-based techniques, and in a very cost-effective way. For most deuterated proteins characterized in the literature, the solubility and thermal stability is reduced. The data reported here is consistent with these observations and it was clear that there are measurable differences between hydrogenous and deuterated versions of the same protein in Tm and how they crystallize.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Effects of anthocyanins and their gut metabolites on adenosine
           diphosphate-induced platelet activation and their aggregation with
           monocytes and neutrophils
    • Authors: Irena Krga; Nevena Vidovic; Dragan Milenkovic; Aleksandra Konic-Ristic; Filip Stojanovic; Christine Morand; Marija Glibetic
      Pages: 34 - 41
      Abstract: Publication date: Available online 16 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Irena Krga, Nevena Vidovic, Dragan Milenkovic, Aleksandra Konic-Ristic, Filip Stojanovic, Christine Morand, Marija Glibetic
      Accumulating evidence suggests that anthocyanins play an important role in the cardioprotective effects associated with consumption of anthocyanin-rich foods. These benefits may partly be attributed to their effects on platelets, significant contributors to cardiovascular disease development. This study aimed to investigate the impact of physiologically relevant concentrations of anthocyanins and their metabolites on platelet activation and platelet-leukocyte aggregation. Whole blood from seven healthy volunteers was treated with anthocyanins: cyanidin-3-arabinoside, cyanidin-3-glucoside, cyanidin-3-galactoside, delphinidin-3-glucoside and peonidin-3-glucoside at 0.1 μM concentration or gut metabolites: 4-hydroxybenzaldehyde, protocatechuic, vanillic, ferulic and hippuric acids at 0.5 μM, 0.2 μM, 2 μM, 1 μM, 2 μM concentration, respectively. Markers of adenosine diphosphate-induced platelet activation (P-selectin and GPIIb-IIIa expression) and platelet-monocyte and platelet-neutrophil aggregation were analyzed using flow cytometry. Cyanidin-3-arabinoside, delphinidin-3-glucoside, and peonidin-3-glucoside decreased agonist-induced P-selectin expression, while cyanidin-3-galactoside and cyanidin-3-arabinoside reduced platelet-neutrophil aggregation. Hippuric and protocatechuic acids inhibited P-selectin expression, ferulic acid reduced platelet-monocyte aggregation, while 4-hydroxybenzaldehyde affected P-selectin expression, platelet-neutrophil and monocyte aggregation. Only cyanidin-3-glucoside and protocatechuic acid decreased GPIIb-IIIa expression. These results demonstrate the bioactivity of anthocyanins and their gut metabolites at physiologically relevant concentrations on platelet function and interaction with leukocytes, presenting mechanisms by which they contribute to the beneficial effects of habitual consumption of anthocyanin-rich foods on cardiovascular health.
      Graphical abstract image

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Antioxidant potentials of nanoceria synthesized by solution plasma process
           and its biocompatibility study
    • Authors: MubarakAli Davoodbasha; Bo-Ram Park; Won-Jong Rhee; Sang-Yul Lee; Jung-Wan Kim
      Pages: 42 - 49
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): MubarakAli Davoodbasha, Bo-Ram Park, Won-Jong Rhee, Sang-Yul Lee, Jung-Wan Kim
      Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0–25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV–Vis spectroscopy showed a characteristic absorbance maxima at 304–320 nm for the nanoceria with increase in the intensity of the peaks as the concentration of Ce(NO3)2 increased. The peaks exhibited transition red shift due to nanoceria formation. High resolution transmission electron microscopy revealed that spherical nanoparticles with an average size of 7.0 ± 0.2 nm were formed by discharging plasma for 15 min. The nanoceria showed excellent pH dependent antioxidant properties in hydroxyl and superoxide anion radical scavenging assays. Effect of the nanoceria on cell viability in vitro and inhibition of reactive oxygen species (ROS) by the nanoceria were examined using HeLa cell lines. As the results, no toxic effect was found up to 1600 μg mL−1 of nanoceria and they had an effective antioxidant property. Therefore, the nanoceria synthesized by one-step solution plasma process without employing hazardous chemicals have potential for utilization as antioxidant biomaterials and sustained release in the stream to scavenge ROS in the modern medicine.
      Graphical abstract image

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira
           pseudonana: Identification of a gene encoding MTHB-methyltransferase
    • Authors: Hakuto Kageyama; Yoshito Tanaka; Ayumi Shibata; Rungaroon Waditee-Sirisattha; Teruhiro Takabe
      Pages: 100 - 106
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Hakuto Kageyama, Yoshito Tanaka, Ayumi Shibata, Rungaroon Waditee-Sirisattha, Teruhiro Takabe
      Dimethylsulfoniopropionate (DMSP) is one of the most abundant molecules on earth and plays a pivotal role in the marine sulfur cycle. DMSP is believed to be synthesized from methionine by a four-step reaction pathway in marine algae. The genes responsible for biosynthesis of DMSP remain unidentified. A diatom Thalassiosira pseudonana CCMP1335 is an important component of marine ecosystems and contributes greatly to the world's primary production. In this study, through genome search, in vivo activity and functional studies of cDNA products, a gene encoding Thalassiosira methyltransferase (TpMMT) which catalyzes the key step of DMSP synthesis formation of 4-methylthio-2-hydroxybutyrate (DMSHB) from 4-methylthio-2-oxobutyrate (MTHB), was identified. The amino acid sequence of TpMMT was homologous to the methyltransferase from Phaeodactylum tricornutum CCAP 1055/1, but not the recently identified bacterium gene. High salinity and nitrogen limitation stresses caused the increase of DMSP content and TpMMT protein in Thalassiosira. In addition to TpMMT, the enzyme activities for the first three steps could be detected and enhanced under high salinity, suggesting the importance of four-step DMSP synthetic pathway in Thalassiosira.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Deciphering protein dynamics changes along the pathway of retinol uptake
           by cellular retinol-binding proteins 1 and 2
    • Authors: Ilaria Menozzi; Eugenia Polverini; Rodolfo Berni
      Pages: 107 - 116
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Ilaria Menozzi, Eugenia Polverini, Rodolfo Berni
      Four Cellular Retinol-binding Proteins (CRBP 1, 2, 3, 4) are encoded in the human genome. CRBP 1 and 2, sharing a 56% amino acid sequence identity, exhibit the highest binding affinities for retinol. Previous NMR studies provided some insights into the mechanism of retinol uptake, but details of such mechanism remain to be elucidated. Herein, the results of molecular dynamics simulations for the uptake of retinol by CRBP 1 and 2 are consistent with the presence of two different retinol entry points, both involving the ‘cap region’ (α-helices I and II and neighboring loops). We observed that a hydrophobic patch at the surface of the ‘portal region’ (α-helix II, CD and EF loops) of CRBP 1 attracts retinol, which accesses the binding cavity through an opening generated by the concerted movements of Arg58 and Phe57, present in the CD loop. In CRBP 2 a different distribution of the surface residues of the ‘cap region’ allows retinol to access the binding cavity by sinking in a hydrophobic matrix between the two α-helices. Polar interactions mainly affect retinol movements inside the β-barrel cavities of both CRBPs. The interaction energy profiles are in agreement with the different behavior of the two protein systems.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • Lymphatic metabolites of quercetin after intestinal administration of
           quercetin-3-glucoside and its aglycone in rats
    • Authors: Toshiyuki Nakamura; Chinatsu Kinjo; Yoshimasa Nakamura; Yoji Kato; Miyu Nishikawa; Masahiro Hamada; Noriyuki Nakajima; Shinichi Ikushiro; Kaeko Murota
      Pages: 126 - 136
      Abstract: Publication date: 1 May 2018
      Source:Archives of Biochemistry and Biophysics, Volume 645
      Author(s): Toshiyuki Nakamura, Chinatsu Kinjo, Yoshimasa Nakamura, Yoji Kato, Miyu Nishikawa, Masahiro Hamada, Noriyuki Nakajima, Shinichi Ikushiro, Kaeko Murota
      Quercetin is a major flavonoid, present as its glycosidic forms in plant foods. In this study, quercetin-3-glucoside (Q3G) was administered intraduodenally to thoracic lymph-cannulated rats, and its lymphatic transport was investigated. The resulting lymphatic and plasma metabolites were identified with LC-MS/MS and compared with those after administration of quercetin aglycone. The total concentration of quercetin metabolites in the lymph was about four times lower than that in the plasma, and quercetin and its methylated form isorhamnetin were detected as their glucuronides, sulfates and diglucuronides both in the lymph and the plasma after Q3G and quercetin administrations. The lymph levels of the glucuronides after Q3G administration were lower than those after quercetin administration, whereas those in the plasma showed the opposite pattern. Both the lymph and plasma levels of the sulfates after Q3G administration were lower than those after quercetin administration. Some of the intestinal metabolites like quercetin monoglucuronides were transported directly into the lymph and the hepatic metabolites like the diglucuronides were eventually transferred from the plasma into the lymph. These results indicate that the absorbed Q3G is partly transported into the intestinal lymph as quercetin metabolites. Deglycosylation in the enterocyte is also suggested to affect the subsequent metabolic pathways.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 645 (2018)
  • ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme
    • Authors: Jiaqi Chu; Yuanyuan Fu; Jiecheng Xu; Xueping Zheng; Qianqian Gu; Xia Luo; Qi Dai; Shuxian Zhang; Peiqing Liu; Liang Hong; Min Li
      Pages: 29 - 36
      Abstract: Publication date: Available online 3 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Jiaqi Chu, Yuanyuan Fu, Jiecheng Xu, Xueping Zheng, Qianqian Gu, Xia Luo, Qi Dai, Shuxian Zhang, Peiqing Liu, Liang Hong, Min Li
      Atg4 is essential for autophagosome formation and Atg8 recycle with the function of processing the precursor and the lipidated Atg8-family proteins. Abnormal autophagic activity is involved in a variety of pathophysiological diseases and ATG4B is of interest as a potential therapeutic target due to its key roles in autophagy process. So ATG4B inhibitors are highly needed. FMK-9a is the most potent inhibitor reported so far. In this study, we confirmed FMK-9a could suppress ATG4B activity in vitro and in cells, with an IC50 of 260 nM. Besides, FMK-9a could also attenuate the process of cleavage of pro-LC3 and the delipidation of LC3-PE. Importantly, FMK-9a could induce autophagy both in HeLa and MEF cells regardless of its inhibition on ATG4B activity. Moreover, FMK-9a induced autophagy required FIP200 and ATG5. In conclusion, we demonstrated that ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Thus, FMK-9a may plays multiple roles in autophagy process and cannot simply take it as an ATG4B inhibitor.
      Graphical abstract image

      PubDate: 2018-03-07T02:08:04Z
      DOI: 10.1016/
      Issue No: Vol. 644 (2018)
  • A glycoprotein from mammary gland secreted during involution promotes
           apoptosis: Structural and biological studies
    • Authors: Anshul Chaudhary; Vinod Kumar; Prashant K. Singh; Pradeep Sharma; Hridoy R. Bairagya; Punit Kaur; Sujata Sharma; Shyam S. Chauhan; Tej P. Singh
      Pages: 72 - 80
      Abstract: Publication date: 15 April 2018
      Source:Archives of Biochemistry and Biophysics, Volume 644
      Author(s): Anshul Chaudhary, Vinod Kumar, Prashant K. Singh, Pradeep Sharma, Hridoy R. Bairagya, Punit Kaur, Sujata Sharma, Shyam S. Chauhan, Tej P. Singh
      Secretory signalling glycoprotein (SPX-40) from mammary gland is highly expressed during involution. This protein is involved in a programmed cell death during tissue remodelling which occurs at the end of lactation. SPX-40 was isolated and purified from buffalo (SPB-40) from the samples obtained during involution. One solution of SPB-40 was made by dissolving it in buffer containing 25 mM Tris-HCl and 50 mM NaCl at pH 8.0. Another solution was made by adding 25% ethanol to the above solution. The biological effects of SPB-40 dissolved in above two solutions were evaluated on MCF-7 breast cancer cell lines. Free SPB-40 indicated significant pro-apoptotic effects while ethanol exposed SPB-40 showed considerably reduced effects on the apoptosis. SPB-40 was crystallized in the native state. The crystals of SPB-40 were soaked in four separate solutions containing 25% acetone, 25% ethanol, 25% butanol and 25% MPD. Four separate data sets were collected and their structures were determined at high resolutions. In all the four structures, the molecules of acetone, ethanol, butanol and MPD respectively were observed in the hydrophobic binding pocket of SPB-40. As a result of which, the conformation of Trp78 was altered thus blocking the binding site in SPB-40 leading to the loss of activity.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 644 (2018)
  • Type 2 diabetes-related proteins derived from an in vitro model of
           inflamed fat tissue
    • Authors: Jean Paul ten Klooster; Alexandros Sotiriou; Sjef Boeren; Stefan Vaessen; Jacques Vervoort; Raymond Pieters
      Pages: 81 - 92
      Abstract: Publication date: 15 April 2018
      Source:Archives of Biochemistry and Biophysics, Volume 644
      Author(s): Jean Paul ten Klooster, Alexandros Sotiriou, Sjef Boeren, Stefan Vaessen, Jacques Vervoort, Raymond Pieters
      Currently, there is a worldwide increase of patients with type 2 diabetes (T2D). During the progression of healthy obese to T2D status, there is an influx of immune cells, in particular macrophages, into visceral adipose tissue, accompanied by an increase of inflammatory cytokines, such as, IL6, TNFα and Hp. To get a better insight in the underlying mechanisms, we performed a quantitative LCMS analysis on a modified in vitro assay, combining 3T3L1 adipocytes and activated RAW264.7 macrophages, thus mimicking inflamed adipose tissue. Clinically known proteins, e.g. IL6, TNFα, AdipoQ, complement factor C3, B and D were identified, thus confirming the assay. In addition, we found 54 new proteins that can potentially be used for research into the mechanism of T2D. Comparison of our results to a study on human visceral fat of obese non-diabetic and obese diabetic subjects, indicated that AUH, NAGK, pCYT2, NNMT, STK39 and CSNK2A2 might indeed be linked to insulin resistance in humans. Moreover, the expression of some of these genes was also altered in human blood samples at early or later stages of insulin desensitization. Overall, we conclude that the direct contact co-culture of 3T3L1 adipocytes with activated macrophages could be a mechanistically relevant and partially translational model of inflamed visceral adipose tissue.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
      Issue No: Vol. 644 (2018)
  • Polyphyllin G exhibits antimicrobial activity and exerts anticancer
           effects on human oral cancer OECM-1 cells by triggering G2/M cell cycle
           arrest by inactivating cdc25C-cdc2
    • Authors: Xiaoqing Cai; Lele Guo; Fei Pei; Xiaoyun Chang; Rui Zhang
      Pages: 93 - 99
      Abstract: Publication date: 15 April 2018
      Source:Archives of Biochemistry and Biophysics, Volume 644
      Author(s): Xiaoqing Cai, Lele Guo, Fei Pei, Xiaoyun Chang, Rui Zhang
      Plant natural products have long been considered to be important sources of bioactive molecules. A large number of antimicrobial and anticancer agents have been isolated form plants. In the present study we evaluated the antimicrobial and anticancer activity of a plant derived secondery metabolite, Polyphyllin G. The results of antibacterial assays showed that Polyphyllin G prevented the growth of both Gram-positive and Gram-negative bacteria with minimum inhibitory concentrations (MICs) ranging from 13.1 to 78 μg/ml. Antifungal activity measured as inhibition of mycelium growth ranged between 38.32 and 56.50%. Further Polyphyllin G was also evaluated against a panel of cancer cell lines. The IC50 of Polyphyllin G ranged from 10 to 65 μM. However the IC50 of Polyphyllin G was found to be comparatively high (120 μM) against the normal FR2 cancer cell line. The lowest IC50 of 10 μM was found against the oral cancer cell line OECM-1. Therefore further studies were carried out on this cell line only. Our results indicated that Polyphyllin G induced cell arrest in oral cancer OECM-1 cells by inactivation of cdc25C-cdc22 via ATM-Chk 1/2 stimulation. Therefore, we propose that Polyphyllin G might prove a lead molecule in the management of oral cancers and at the same time may prevent the growth of opportunistic microbes.

      PubDate: 2018-04-15T05:42:57Z
      DOI: 10.1016/
      Issue No: Vol. 644 (2018)
  • OR2H2 regulates the differentiation of human myoblast cells by its ligand
           aldehyde 13-13
    • Authors: Benjamin Kalbe; Markus Osterloh; Viola M. Schulz; Janine Altmüller; Christian Becker; Sabrina Osterloh; Hanns Hatt
      Abstract: Publication date: Available online 17 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Benjamin Kalbe, Markus Osterloh, Viola M. Schulz, Janine Altmüller, Christian Becker, Sabrina Osterloh, Hanns Hatt
      Olfactory receptors (ORs) regulate various cellular processes in the human body. The receptors' participation in physiological and pathophysiological processes could be demonstrated in several studies. In addition to the regulation of sperm motility, respiratory physiology, and heart contraction, ORs play a crucial role in cancer cells. In murine myoblasts, mOR23 regulates the myogenesis and branching of skeletal muscle cells. To date, the expression and physiological role of ORs in human skeletal muscle cells have not been thoroughly elucidated. We demonstrate that four different ORs are expressed at the transcript level in differentiated myoblasts, and one other OR is expressed in undifferentiated myoblasts. Moreover, we characterized the expression of OR2H2 in differentiated human myoblasts and identified a specific ligand, aldehyde 13-13. We could observe a concentration-dependent Ca2+ increase in differentiated human myoblasts upon aldehyde 13-13 stimulation, which is mediated by PI3K signaling. Aldehyde 13-13 has a reducing effect on myoblast fusion. We conclude that OR2H2 could have a regulatory role in myoblast differentiation. To the best of our knowledge, this report presents the first verification of the expression of ORs in human myoblasts. OR2H2 might be an interesting candidate for playing a role in the complex mechanism of myogenesis.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Comparison of different analytical techniques for the analysis of
           carotenoids in tamarillo (Solanum betaceum Cav.)
    • Authors: Daniele Giuffrida; Mariosimone Zoccali; Adriana Arigò; Francesco Cacciola; Coralia Osorio Roa; Paola Dugo; Luigi Mondello
      Abstract: Publication date: Available online 17 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Daniele Giuffrida, Mariosimone Zoccali, Adriana Arigò, Francesco Cacciola, Coralia Osorio Roa, Paola Dugo, Luigi Mondello
      An on-line method based on the coupling of supercritical fluid extraction and supercritical fluid chromatography with triple quadrupole mass spectrometry detection (SFESFCQqQ/MS) for the native carotenoids characterization and apocarotenoids detection in yellow tamarillo was developed for the first time; this is the first work reporting the application of this methodology for the apocarotenoids analysis in any matrix. An off-line liquid extraction (LE) and analysis by LC-PDA-MS, using both a conventional C30 (3 μm I.D. particles) and a novel C30 column with sub-2 μm particles were also performed. 31 compounds were extracted and identified by the developed SFESFCQqQ/MS methodology in less than 18 min, including free carotenoids, carotenoids monoesters, carotenoids diesters and apocarotenoids in a very fast, and efficient way; moreover among those 31 compounds, 3 antheraxanthin monoesters and 9 apocarotenoids were detected in yellow tamarillo for the first time, namely apo-8′-zeaxanthinal, apo-10′-zeaxanthinal, apo-12′-zeaxanthinal, apo-14′-zeaxanthinal, apo-15′-zeaxanthinal, apo-12′-carotenal, apo-14′-carotenal and the two apocarotenoids esters apo-10′-zeaxanthinal-C4:0 and apo-8′-zeaxanthinal-C12:0. Further, the novel sub-2 μm particles C30 column, showed a better performance compared to the conventional C30 one. Finally a quantitative comparison of two selected carotenoids was performed by using SFESFCQqQ/MS, SFC-QqQ/MS, and LC-PDA, which showed overall comparable results.
      Graphical abstract image

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Long non-coding RNA ROR promotes radioresistance in hepatocelluar
           carcinoma cells by acting as a ceRNA for microRNA-145 to regulate RAD18
    • Authors: Ying Chen; Zetian Shen; Yingru Zhi; Hao Zhou; Kai Zhang; Ting Wang; Bing Feng; Yitian Chen; Haizhu Song; Rui Wang; Xiaoyuan Chu
      Abstract: Publication date: Available online 17 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Ying Chen, Zetian Shen, Yingru Zhi, Hao Zhou, Kai Zhang, Ting Wang, Bing Feng, Yitian Chen, Haizhu Song, Rui Wang, Xiaoyuan Chu
      Radiotherapy plays a limited role in the treatment of hepatocellular carcinoma (HCC) due to the development of resistance. Therefore, further investigation of underlying mechanisms involved in HCC radioresistance is warranted. Increasing evidence shows that long non-coding RNAs (linc-RNAs) are involved in the pathology of various tumors, including HCC. Previously, we have shown that long noncoding RNA regulator of reprogramming (linc-ROR) promotes HCC metastasis via induction of epithelial-mesenchymal transition (EMT). However, the roles of linc-ROR in HCC radioresistance and its possible mechanisms are unclear. Here, we established two radioresistant HCC cell lines (HepG2-R and SMMC-7721-R) and found that linc-ROR was significantly upregulated in radioresistant HCC cells. Knockdown of linc-ROR reduces in vitro and in vivo radiosensitivity of parental HCC cells by reducing DNA repair capacity, while ectopic expression of linc-ROR enhances radiosensitivity of radioresistant HCC cells. Further mechanistic investigations revealed that lincRNA-ROR exerted its biological effects by acting as a competing endogenous RNA (ceRNA) for miR-145 to regulate RAD18 expression, thereby promoting DNA repair. Collectively, our findings demonstrate that linc-ROR promotes HCC radioresistance and targeting it will be a promising strategy for enhancing the efficacy of radiotherapies in HCC.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Network analytics approach towards identifying potential antivirulence
           drug targets within the Staphylococcus aureus staphyloxanthin biosynthetic
    • Authors: Marni E. Cueno; Kenichi Imai
      Abstract: Publication date: Available online 15 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Marni E. Cueno, Kenichi Imai
      Staphylococcus aureus is associated with several clinically significant infections among humans and infections associated with antibiotic-resistant strains are growing in frequency. Antivirulence strategies shift the target of drugs from bacterial growth to the infection process resulting to milder evolutionary pressure for the development of bacterial resistant strains. Staphyloxanthin (STX) is a yellowish-orange carotenoid pigment synthesized by S. aureus and this carotenoid functions as an important virulence factor for the bacteria. In this study, we elucidated whether network analytics can be used as a viable tool to identify significant components in the STX biosynthetic network which in-turn could serve as possible antivirulence drug targets. For confirmation, we correlated our results to known drugs that were able to inhibit STX biosynthesis. Throughout this study, we established that crtN(1) activity and 4,4′-diaponeurosporene amounts are significant components in the STX biosynthetic network and, moreover, network analytics can aid in identifying antivirulence drug targets within the STX biosynthetic network. Similarly, we found that network analytics is capable of identifying multiple potential targets simultaneously. Taken together, we propose that an effective antivirulence drug against S. aureus STX biosynthesis would involve targeting crtN(1) activity, 4,4′-diaponeurosporene levels, or both components.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • miR-19b-3p inhibits breast cancer cell proliferation and reverses
           saracatinib-resistance by regulating PI3K/Akt pathway
    • Authors: Juan Jin; Zijia Sun; Fang Yang; Lin Tang; Weiwei Chen; Xiaoxiang Guan
      Abstract: Publication date: Available online 14 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Juan Jin, Zijia Sun, Fang Yang, Lin Tang, Weiwei Chen, Xiaoxiang Guan
      Breast cancer arises as the most frequent malignancy, and causes the majority of cancer death among females worldwide. Src is a tyrosine kinase identified as the product of the proto-oncogene and is supposed to promote cancer development and metastasis. Src inhibitors are recently developed and have shown efficacy in breast cancer. Increasing evidences suggest that aberrant expression of miRNAs is involved in cancer development and drug resistance. Identifying miRNAs associated with drug resistance may enhance the sensitivity of targeted therapies, including Src inhibitors. In this study, we established a Src inhibitor saracatinib-resistant breast cancer cell line (SK-BR-3/SI) for the first time. Microarray data and qRT-PCR results showed that miR-19b-3p expression was downregulated in saracatinib-resistant cells compared with saracatinib-sensitive cells. Downregulation of miR-19b-3p remarkably increased the IC50 value of saracatinib, and promoted cell migration. Further studies found that miR-19b-3p reduced PIK3CA expression by directly targeting PIK3CA gene and the resistance of Src inhibitor might be associated with activation of PI3K/Akt pathway after downregulation of miR-19b-3p. Moreover, we demonstrated that PI3K inhibitor LY294002 could reverse saracatinib resistance in saracatinib-resistant cells, which deserved further preclinical and clinical evaluation of dual inhibition of Src and PI3K in breast cancer.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Myeloperoxidase in the inflamed colon: A novel target for treating
           inflammatory bowel disease
    • Authors: Belal Chami; Nathan J.J. Martin; Joanne M. Dennis; Paul K. Witting
      Abstract: Publication date: Available online 13 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Belal Chami, Nathan J.J. Martin, Joanne M. Dennis, Paul K. Witting
      Inflammatory bowel disease (IBD) is a debilitating disorder involving inflammation of the gastrointestinal tract. The incidence of IBD is increasing worldwide. Immunological responses in the gastrointestinal (GI) tract to altered gut microbiota, mucosal injury and loss of intestinal epithelial cell function all contribute to a complex mechanism underlying IBD pathogenesis. Immune cell infiltration, particularly neutrophils, is a histological feature of IBD. This innate immune response is aimed at resolving intestinal damage however, neutrophils and monocytes that are recruited and accumulate in the GI wall, participate in IBD pathogenesis by producing inflammatory cytokines and soluble mediators such as reactive oxygen species (ROS; one- and two-electron oxidants). Unregulated ROS production in host tissue is linked to oxidative damage and inflammation and may potentiate mucosal injury. Neutrophil-myeloperoxidase (MPO) is an abundant granule enzyme that catalyses production of potent ROS; biomarkers of oxidative damage (and MPO protein) are increased in the mucosa of patients with IBD. Targeting MPO may mitigate oxidative damage to host tissue and ensuing inflammation. Here we identify mechanisms by which MPO activity perpetuates inflammation and contributes to host-tissue injury in patients with IBD and discuss MPO as a potential therapeutic target to protect the colon from inflammatory injury.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Non-conventional rottlerin anticancer properties
    • Authors: Maioli E; Daveri E; Maellaro E; Ietta F; Cresti L; Valacchi G
      Abstract: Publication date: Available online 12 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Maioli E, Daveri E, Maellaro E, Ietta F, Cresti L, Valacchi G
      In the past few years, we focused the interest on rottlerin, an old/new natural substance that, over the time, has revealed a number of cellular and molecular targets, all potentially implicated in the fight against cancer. Past and recent literature well demonstrated that rottlerin is an inhibitor of enzymes, transcription factors and signaling molecules that control cancer cell life and death. Although the rottlerin anticancer activity has been mainly ascribed to apoptosis and/or autophagy induction, recent findings unveiled the existence of additional mechanisms of toxicity. The major novelties highlighted in this mini review are the ability to bind and inhibit key molecules, such as ERK and mTOR, directly, thus independently of upstream signaling cascades, and to cause a profound dysregulation of cap-dependent protein translation through the mTORC1/4EBP1/eIF4E axis and by inhibition of eIF2, an initiation factor of translation that is negatively regulated by endoplasmic reticulum (ER) stress. These last mechanisms, proved to be lethal in cancer cell lines derived from breast and skin, strongly enforce the potential of rottlerin as a promising natural lead compound for the development of novel therapeutic approaches.
      Graphical abstract image

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Biophysical and biochemical studies on glycoxidatively modified human low
           density lipoprotein
    • Authors: Minhal Abidi; M. Salman Khan; Saheem Ahmad; Tasneem Kausar; Shahid M. Nayeem; Sidra Islam; Asif Ali; Khursheed Alam; Moinuddin
      Abstract: Publication date: Available online 8 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Minhal Abidi, M. Salman Khan, Saheem Ahmad, Tasneem Kausar, Shahid M. Nayeem, Sidra Islam, Asif Ali, Khursheed Alam, Moinuddin
      Methylglyoxal (MGO), a reactive dicarbonyl metabolite is a potent arginine directed glycating agent which has implications for diabetes-related complications. Dicarbonyl metabolites are produced endogenously and in a state of misbalance, they contribute to cell and tissue dysfunction through protein and DNA modifications causing dicarbonyl stress. MGO is detoxified by glyoxalase 1 (GLO1) system in the cytoplasm. Reactive oxygen species (ROS) are known to aggravate the glycation process. Both the processes are closely linked, and their combined activity is often referred to as “glycoxidation” process. Glycoxidation of proteins has several consequences such as type 2 diabetes mellitus (T2DM), aging etc. In this study, we have investigated the glycation of low-density lipoprotein (LDL) using different concentrations of MGO for varied incubation time periods. The structural perturbations induced in LDL were analyzed by UV–Vis, fluorescence, circular dichroism spectroscopy, molecular docking studies, polyacrylamide gel electrophoresis, FTIR, thermal denaturation studies, Thioflavin T assay and isothermal titration calorimetry. The ketoamine moieties, carbonyl content and HMF content were quantitated in native and glycated LDL. Simulation studies were also done to see the effect of MGO on the secondary structure of the protein. We report structural perturbations, increased carbonyl content, ketoamine moieties and HMF content in glycated LDL as compared to native analog (native LDL). We report the structural perturbations in LDL upon modification with MGO which could obstruct its normal physiological functions and hence contribute to disease pathogenesis and associated complications.

      PubDate: 2018-03-19T03:04:47Z
      DOI: 10.1016/
  • Non-thermal atmospheric pressure plasma-induced IL-8 expression is
           regulated via intracellular K+ loss and subsequent ERK activation in human
           keratinocyte HaCaT cells
    • Authors: Emiri Hotta; Hirokazu Hara; Tetsuro Kamiya; Tetsuo Adachi
      Abstract: Publication date: Available online 6 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Emiri Hotta, Hirokazu Hara, Tetsuro Kamiya, Tetsuo Adachi
      Non-thermal atmospheric pressure plasma (NTAPP) has recently emerged as a novel medical therapy for skin wounds. Interleukin-8 (IL-8) is thought to play a critical role in wound healing. NTAPP irradiation has been reported to promote production of IL-8; however, the mechanism is not fully understood. The aim of this study was to elucidate the underlying mechanism of NTAPP-induced IL-8 expression in human keratinocyte HaCaT cells. NTAPP irradiation of HaCaT cells increased IL-8 mRNA expression in an irradiation time-dependent manner. Although hydrogen peroxide (H2O2) was generated in culture medium irradiated with NTAPP, treatment of HaCaT cells with H2O2 itself failed to induce the expression. In addition, we found that NTAPP irradiation of HaCaT cells decreased intracellular K+ levels. High intracellular K+ concentrations suppressed NTAPP-induced IL-8 mRNA expression, and the K+ ionophore valinomycin (Val) enhanced the induction of IL-8 mRNA. Moreover, NTAPP stimulated activation of ERK MAP kinase and the ERK inhibitor prevented NTAPP-induced IL-8 mRNA expression. NTAPP-induced ERK activation was inhibited in the presence of high concentrations of extracellular K+ and enhanced in the presence of Val. Taken together, these findings suggest that NTAPP irradiation stimulates intracellular K+ loss and subsequent ERK activation, leading to the induction of IL-8 expression.

      PubDate: 2018-03-07T02:08:04Z
      DOI: 10.1016/
  • Impact of polyphenols on extracellular vesicle levels and effects and
           their properties as tools for drug delivery for nutrition and health
    • Authors: Raffaella Soleti; Ramaroson Andriantsitohaina; M. Carmen Martinez
      Abstract: Publication date: Available online 5 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Raffaella Soleti, Ramaroson Andriantsitohaina, M. Carmen Martinez
      Polyphenols are found in plant-derived foods and beverages and display numerous protective effects against cancers, cardiovascular, metabolic and neurodegenerative diseases. Extracellular vesicles (EVs), microparticles, exosomes, and apoptotic bodies, originated by different cell types are emerging as a novel mean of cell-to-cell communication in physiology and pathology and represent a new way to convey fundamental information between cells. Polyphenols can act on signaling pathways that interfere with the biogenesis of EVs. Thus, they are able to control EV release from cells and their content and therefore their functional properties. Both EVs and polyphenols are therapeutic tools that can be used against several diseases. In this context, the combination of both tools can increase their therapeutic potential. Three types of strategies can be used: (i) plants are able to produce EVs that encapsulate natural components from vegetables, polyphenols for instance, (ii) mammalian cells can be treated with polyphenols and the subsequent EVs produced are enriched in these components, and (iii) EVs from mammalian cells can be uploaded with polyphenols. We review the novel aspects of the interplay between polyphenols and EVs that could trigger and improve the health benefits in cancer, cardiovascular, metabolic and neurodegenerative diseases.

      PubDate: 2018-03-07T02:08:04Z
      DOI: 10.1016/
  • The primary cause of muscle disfunction associated with substitutions
           E240K and R244G in tropomyosin is aberrant behavior of tropomyosin and
           response of actin and myosin during ATPase cycle
    • Authors: Armen O. Simonyan; Vladimir V. Sirenko; Olga E. Karpicheva; Katarzyna Robaszkiewicz; Małgorzata Śliwinska; Joanna Moraczewska; Zoya I. Krutetskaya; Yurii S. Borovikov
      Abstract: Publication date: Available online 3 March 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Armen O. Simonyan, Vladimir V. Sirenko, Olga E. Karpicheva, Katarzyna Robaszkiewicz, Małgorzata Śliwinska, Joanna Moraczewska, Zoya I. Krutetskaya, Yurii S. Borovikov
      Using the polarized photometry technique we have studied the effects of two amino acid replacements, E240K and R244G, in tropomyosin (Tpm1.1) on the position of Tpm1.1 on troponin-free actin filaments and the spatial arrangement of actin monomers and myosin heads at various mimicked stages of the ATPase cycle in the ghost muscle fibers. E240 and R244 are located in the C-terminal, seventh actin-binding period, in f and b positions of the coiled-coil heptapeptide repeat. Actin, Tpm1.1, and myosin subfragment-1 (S1) were fluorescently labeled: 1.5-IAEDANS was attached to actin and S1, 5-IAF was bound to Tpm1.1. The labeled proteins were incorporated in the ghost muscle fibers and changes in polarized fluorescence during the ATPase cycle have been measured. It was found that during the ATPase cycle both mutant tropomyosins occupied a position close to the inner domain of actin. The relative amount of the myosin heads in the strongly-bound conformations and of the switched on actin monomers increased at mimicking different stages of the ATPase cycle. This might be one of the reasons for muscle dysfunction in congenital fiber type disproportion caused by the substitutions E240K and R244G in tropomyosin.
      Graphical abstract image

      PubDate: 2018-03-07T02:08:04Z
      DOI: 10.1016/
  • β- Adrenoceptors activate hepatic glutathione efflux through an
           unreported pathway
    • Authors: Deyamira Matuz-Mares; Alain Hernández-Vázquez; Héctor Riveros-Rosas; Raquel Guinzberg; Tania Quesada-López; Alfonso Cárabez-Trejo; Ofelia Mora; Enrique Piña
      Abstract: Publication date: Available online 26 February 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Deyamira Matuz-Mares, Alain Hernández-Vázquez, Héctor Riveros-Rosas, Raquel Guinzberg, Tania Quesada-López, Alfonso Cárabez-Trejo, Ofelia Mora, Enrique Piña
      The physiological regulation of hepatic glutathione efflux by catecholamines is poorly understood. The purpose of this work was to review the role of adrenergic receptors (AR) on total glutathione (GT) efflux in rat liver. Two models were used: isolated hepatocytes and perfused livers. In hepatocytes 10 μM adrenaline (Adr), but not isoproterenol (Iso) a β-AR agonist, or phenylephrine (Phe) an α1-AR agonist, (in a Krebs-Henseleit Buffer (KHB) enriched with Ca2+ and some aminoacids) increased in 13% GT efflux. In livers perfused with KHB, Adr or Iso at 1 μmolar doses (but not Phe) stimulated 11-fold initial velocity of GT release, but only during the first 2 min of perfusion. This immediate response progressively disappeared during the following 15 min of perfusion. A second phase of GT efflux, observed between 2 and 14 min of perfusion, mimics the one reported earlier in isolated hepatocytes. The ED 50 for Adr and Iso activation are in the range of 320 nM and 10 nM, respectively. Iso-mediated GT release requires Ca2+ to work, and was prevented by H89, glibenclamide, cystic fibrosis transmembrane regulator (CFTR) antibodies, and a direct CFTR inhibitor. This short-lived GT release system is associated to PKA activation and probably operates through CFTR.

      PubDate: 2018-03-07T02:08:04Z
      DOI: 10.1016/
  • Biochemical and functional insights on the triheme cytochrome PpcA from
           Geobacter metallireducens
    • Authors: Pilar C. Portela; Tomás M. Fernandes; Joana M. Dantas; Marisa R. Ferreira; Carlos A. Salgueiro
      Abstract: Publication date: Available online 25 February 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Pilar C. Portela, Tomás M. Fernandes, Joana M. Dantas, Marisa R. Ferreira, Carlos A. Salgueiro
      G. metallireducens bacterium has highly versatile respiratory pathways that provide the microorganism an enormous potential for many biotechnological applications. However, little is known about the structural and functional properties of its electron transfer components. In this work, the periplasmic cytochrome PpcA from G. metallireducens was studied in detail for the first time using complementary biophysical techniques, including UV–visible, CD and NMR spectroscopy. The results obtained showed that PpcA contains three low-spin c-type heme groups with His-His axial coordination, a feature also observed for its homologue in G. sulfurreducens. However, despite the high sequence homology between the two cytochromes, important structural and functional differences were observed. The comparative analysis of the backbone, side chain and heme substituents NMR signals revealed differences in the relative orientation of the hemes I and III. In addition, redox titrations followed by visible spectroscopy showed that the redox potential values for PpcA from G. metallireducens (−78 and −93 mV at pH 7 and 8, respectively) are considerably less negative. Overall, this study provides biochemical and biophysical data of a key cytochrome from G. metallireducens, paving the way to understand the extracellular electron transfer mechanisms in these bacteria.

      PubDate: 2018-02-26T01:31:21Z
      DOI: 10.1016/
  • Solution conformation of a cohesin module and its scaffoldin linker from a
           prototypical cellulosome
    • Authors: Albert Galera-Prat; David Pantoja-Uceda; Douglas V. Laurents; Mariano Carrión Vázquez
      Abstract: Publication date: Available online 24 February 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Albert Galera-Prat, David Pantoja-Uceda, Douglas V. Laurents, Mariano Carrión Vázquez
      Bacterial cellulases are drawing increased attention as a means to obtain plentiful chemical feedstocks and fuels from renewable lignocellulosic biomass sources. Certain bacteria deploy a large extracellular multi-protein complex, called the cellulosome, to degrade cellulose. Scaffoldin, a key non-catalytic cellulosome component, is a large protein containing a cellulose-specific carbohydrate-binding module and several cohesin modules which bind and organize the hydrolytic enzymes. Despite the importance of the structure and protein/protein interactions of the cohesin module in the cellulosome, its structure in solution has remained unknown to date. Here, we report the backbone 1H, 13C and 15N NMR assignments of the Cohesin module 5 from the highly stable and active cellulosome from Clostridium thermocellum. These data reveal that this module adopts a tightly packed, well folded and rigid structure in solution. Furthermore, since in scaffoldin, the cohesin modules are connected by linkers we have also characterized the conformation of a representative linker segment using NMR spectroscopy. Analysis of its chemical shift values revealed that this linker is rather stiff and tends to adopt extended conformations. This suggests that the scaffoldin linkers act to minimize interactions between cohesin modules. These results pave the way towards solution studies on cohesin/dockerin's fascinating dual-binding mode.

      PubDate: 2018-02-26T01:31:21Z
      DOI: 10.1016/
  • Ferrochelatase π-helix: Implications from examining the role of the
    • Authors: Mallory E. Gillam; Gregory A. Hunter; Gloria C. Ferreira
      Abstract: Publication date: Available online 23 February 2018
      Source:Archives of Biochemistry and Biophysics
      Author(s): Mallory E. Gillam, Gregory A. Hunter, Gloria C. Ferreira
      Protoporphyrin ferrochelatase catalyzes the insertion of Fe2+ into protoporphyrin IX to form heme. To determine whether a conserved, active site π-helix contributes to the translocation of the metal ion substrate to the ferrochelatase-bound porphyrin substrate, the invariant π-helix glutamates were replaced with amino acids with non-negatively charged side chains, and the kinetic mechanisms of the generated variants were examined. Analysis of yeast wild-type ferrochelatase-, E314Q- and E318Q-catalyzed reactions, under multi- and single-turnover conditions, demonstrated that the mutations of the π-helix glutamates hindered both protoporphyrin metalation and release of the metalated porphyrin, by slowing each step by approximately 30–50%. Protoporphyrin metalation occurred with an apparent pK a of 7.3 ± 0.1, which was assigned to binding of Fe2+ by deprotonated Glu-314 and Glu-314-assisted Fe2+ insertion into the porphyrin ring. We propose that unwinding of the π-helix concomitant with the adoption of a protein open conformation positions the deprotonated Glu-314 to bind Fe2+ from the surface of the enzyme. Transition to the closed conformation, with π-helix winding, brings Glu-314-bound Fe2+ to the active site for incorporation into protoporphyrin.
      Graphical abstract image

      PubDate: 2018-02-26T01:31:21Z
      DOI: 10.1016/
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