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LABORATORY AND EXPERIMENTAL MEDICINE (99 journals)

Showing 1 - 99 of 99 Journals sorted alphabetically
AAPS PharmSciTech     Hybrid Journal   (Followers: 9)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 7)
Adipocyte     Open Access   (Followers: 1)
African Journal of Laboratory Medicine     Open Access   (Followers: 2)
American Journal of Experimental and Clinical Research     Open Access   (Followers: 4)
American Journal of Medical and Biological Research     Open Access   (Followers: 10)
Animal Models and Experimental Medicine     Open Access  
Annals of Clinical Chemistry and Laboratory Medicine     Open Access   (Followers: 5)
Applied In Vitro Toxicology     Hybrid Journal   (Followers: 2)
Archives of Clinical and Experimental Medicine     Open Access  
Archives of Medical Research     Hybrid Journal   (Followers: 3)
Archives of Pathology & Laboratory Medicine     Full-text available via subscription   (Followers: 31)
Archives of Preventive Medicine     Open Access   (Followers: 3)
Biomedical Engineering     Hybrid Journal   (Followers: 3)
Bulletin of Experimental Biology and Medicine     Hybrid Journal  
Clinica Chimica Acta     Hybrid Journal   (Followers: 30)
Clinical & Experimental Metastasis     Hybrid Journal  
Clinical and Experimental Medical Journal     Full-text available via subscription   (Followers: 1)
Clinical and Experimental Medicine     Hybrid Journal   (Followers: 4)
Clinical Trials     Hybrid Journal   (Followers: 21)
Clinical Trials in Degenerative Diseases     Open Access  
Clinical Trials in Orthopedic Disorders     Open Access   (Followers: 1)
Current Medicine Research and Practice     Full-text available via subscription  
Current Research in Drug Discovery     Open Access   (Followers: 1)
Drug Design, Development and Therapy     Open Access   (Followers: 4)
Ecography     Hybrid Journal   (Followers: 28)
European Journal of Hospital Pharmacy : Science and Practice (EJHP)     Hybrid Journal   (Followers: 8)
European Journal of Medical Research     Open Access   (Followers: 1)
European Journal of Nanomedicine     Hybrid Journal   (Followers: 1)
Experimental & Molecular Medicine     Open Access   (Followers: 1)
Experimental Aging Research: An International Journal Devoted to the Scientific Study of the Aging Process     Hybrid Journal   (Followers: 3)
Experimental and Therapeutic Medicine     Full-text available via subscription   (Followers: 1)
Experimental Biology and Medicine     Hybrid Journal   (Followers: 3)
Expert Opinion on Drug Delivery     Hybrid Journal   (Followers: 20)
Frontiers in Laboratory Medicine     Open Access  
Frontiers in Medical Technology     Open Access   (Followers: 1)
IN VIVO     Full-text available via subscription   (Followers: 5)
International Archives of Biomedical and Clinical Research     Open Access  
International Journal of Experimental Pathology     Hybrid Journal   (Followers: 1)
International Journal of Health Research and Innovation     Open Access   (Followers: 1)
International Journal of Research in Medical Sciences     Open Access   (Followers: 5)
International Journal of Statistics in Medical Research     Hybrid Journal   (Followers: 5)
Journal of Cell Science & Therapy     Open Access   (Followers: 1)
Journal of Applied Biomaterials & Functional Materials     Hybrid Journal   (Followers: 1)
Journal of Biomedical and Clinical Research     Open Access  
Journal of Clinical Laboratory Analysis     Open Access   (Followers: 14)
Journal of Clinical Medicine and Research     Open Access  
Journal of Clinical Medicine Research     Open Access   (Followers: 4)
Journal of Clinical Trials     Open Access   (Followers: 6)
Journal of Current and Advance Medical Research     Open Access   (Followers: 1)
Journal of Current Medical Research and Practice     Open Access  
Journal of Current Research in Scientific Medicine     Open Access  
Journal of Current Researches on Health Sector     Open Access  
Journal of Drug Delivery and Therapeutics JDDT     Open Access   (Followers: 1)
Journal of Enzyme Inhibition and Medicinal Chemistry     Open Access   (Followers: 4)
Journal of Experimental & Clinical Medicine     Full-text available via subscription   (Followers: 1)
Journal of Experimental & Clinical Cancer Research     Open Access   (Followers: 2)
Journal of Experimental and Clinical Medicine     Open Access  
Journal of Experimental Medicine     Full-text available via subscription   (Followers: 45)
Journal of Experimental Pharmacology     Open Access   (Followers: 2)
Journal of Histotechnology     Hybrid Journal   (Followers: 2)
Journal of International Medical Research     Open Access   (Followers: 3)
Journal of Investigative Medicine High Impact Case Reports     Open Access  
Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
Journal of Muhammadiyah Medical Laboratory Technologist     Open Access  
Journal of Operating Department Practitioners     Full-text available via subscription   (Followers: 2)
Journal of the American Society of Cytopathology     Hybrid Journal   (Followers: 6)
Journal of Trace Elements in Medicine and Biology     Hybrid Journal   (Followers: 1)
Lab on a Chip     Full-text available via subscription   (Followers: 42)
Laboratory Investigation     Hybrid Journal   (Followers: 3)
Medical Devices & Sensors     Hybrid Journal  
Medical Image Analysis     Hybrid Journal   (Followers: 15)
Medical Instrumentation     Open Access  
Medical Laboratory Observer     Full-text available via subscription  
Medical Laboratory Technology Journal     Open Access  
Medicinal Chemistry Research     Hybrid Journal   (Followers: 12)
Medtech Insight     Full-text available via subscription   (Followers: 4)
Nanomedicine: Nanotechnology, Biology and Medicine     Hybrid Journal   (Followers: 7)
New Zealand Journal of Medical Laboratory Science     Full-text available via subscription   (Followers: 1)
Oriental Pharmacy and Experimental Medicine     Partially Free   (Followers: 3)
Pathology and Laboratory Medicine International     Open Access   (Followers: 7)
Physical Biology     Hybrid Journal   (Followers: 4)
Practical Laboratory Medicine     Open Access   (Followers: 2)
Proceedings of the Institution of Mechanical Engineers Part H: Journal of Engineering in Medicine     Hybrid Journal   (Followers: 3)
Prosthetics and Orthotics International     Hybrid Journal   (Followers: 9)
Pulse     Full-text available via subscription  
Qualitative Research in Medicine & Healthcare     Open Access  
Recent Advances in Biology and Medicine     Open Access  
Regulatory Toxicology and Pharmacology     Hybrid Journal   (Followers: 43)
Reproduction     Full-text available via subscription   (Followers: 7)
Revista Peruana de Medicina Experimental y Salud Pública     Open Access  
Revista Romana de Medicina de Laborator     Open Access  
RSC Medicinal Chemistry     Full-text available via subscription   (Followers: 6)
SA Pharmacist's Assistant     Open Access  
Savannah Journal of Medical Research and Practice     Full-text available via subscription  
SLAS Technology     Hybrid Journal   (Followers: 2)
Statistics in Medicine     Hybrid Journal   (Followers: 190)
Trends in Molecular Medicine     Full-text available via subscription   (Followers: 14)
Turkish Journal of Clinics and Laboratory     Open Access   (Followers: 1)
Similar Journals
Journal Cover
Oriental Pharmacy and Experimental Medicine
Journal Prestige (SJR): 0.169
Number of Followers: 3  
 
  Partially Free Journal Partially Free Journal
ISSN (Print) 1598-2386 - ISSN (Online) 2211-1069
Published by Springer-Verlag Homepage  [2626 journals]
  • Comparative potential of Simvastatin, Rosuvastatin and Fluvastatin against
           bacterial infection: an in silico and in vitro study
    • Abstract: In the current investigation, we have compared the potential of statin drugs (Simvastatin, Rosuvastatin and Fluvastatin) as potential anti-bacterial agent by conducting in silico, in vitro and ex vivo studies. In silico study was conducted to check the interaction of statin drugs towards various targets of bacteria. The percentage growth retardation, bacterial growth kinetics, MIC determination, post antibiotic effect and biofilm formation assay were conducted to check the anti-bacterial effect of statin drugs under in vitro conditions. Finally, MTT assay was used to check the percentage of immune cell viability after Simvastatin treatment. Docking studies have revealed good interaction of Simvastatin, Rosuvastatin and Fluvastatin towards various targets of bacterial strains as that of the internal ligand. Simvastatin has shown good antibacterial activity against S. aureus, B. pumilus, P. aeruginosa and S. enterica as compared to Rosuvastatin and Fluvastatin. In vitro results have shown concentration and time dependent inhibition of bacterial growth by Simvastatin in concentration range of 64–256 μg/ml. Finally, MTT assay have shown non-cytotoxic effect of Simvastatin against adaptive immune system. In conclusion, Simvastatin could be a potential candidate as an anti-bacterial agent against a wide range of bacterial infections. However, further studies are required to check its complete role before starting phase I clinical trial.
      PubDate: 2019-03-16
       
  • Formulation development and pharmacological evaluation of fixed dose
           combination of Bombyx mori coccon shell extract, Flaxseed oil and
           coenzyme Q10 against doxorubicin induced cardiomyopathy in rats
    • Abstract: The present study aims at formulation development and pharmacological evaluation of fixed dose combination of Bombyx mori (Abresham) extract, Flaxseed oil and coenzyme Q10 (CoQ10) against doxorubicin induced myocardial toxicity in rats. Formulation (emulsion) was prepared using dry gum method (continental method), by using pestle and mortar. The formulation was characterized by performing stability studies which includes flocculation and creaming, cracking, phase inversion and accelerated studies (temperature and light). In-vivo pharmacological evaluation of Bombyx mori coccon shell extract, Flaxseed oil, CoQ10 and its fixed dose combination (FDC) were then performed. Results obtained indicates that developed FDC and extract, Oil, CoQ10 passed all stability tests and significantly prevented drug induced increase in serum levels of AST, ALT, LDH, Creatinine and lipid profile (TC, LDL, VLDL and TG) and increases the levels of HDL and antioxidant parameters—SOD, GSH and CAT (in heart tissue). It also lowered the doxorubicin induced increase in heart weight due to hypertrophy. These results were also confirmed by histopathology. The results of this study strongly indicate the cardioprotective effect of fixed dose combination of BME, Flaxseed oil and CoQ10 against doxorubicin induced myocardial toxicity.
      PubDate: 2019-03-16
       
  • Caesalpinia sappan L. heartwood ethanolic extract exerts genotoxic
           inhibitory and cytotoxic effects
    • Abstract: Brazilin and brazilein, the major compounds of Caesalpinia sappan L. (CS) have been reported to possess antioxidant and cytotoxic activities and could potentially be used as an antigenotoxic as well as an anticancer. This study was conducted to investigate the cytotoxic and antigenotoxic effects of CS ethanolic extract (CEE). In vivo mammalian micronucleus test of CEE at the dose of 500 mg/kg BW and 1000 mg/kg BW decreased the number of MNPCE and increased the ratio of PCE to NCE meaning that CEE performed antigenotoxic effect in an in vivo model. In contrast, CEE and doxorubicin (DOXO) performed cytotoxic effect on CHO-K1 cells under MTT assay with IC50 values of 67 μg/mL and 6 μM, respectively. Interestingly, treatment of CEE in combination with DOXO and H2O2 as genotoxic inducer decreased intracellular ROS levels. In addition, in vitro genotoxicity study by using cytokinesis-block micronucleus (CBMN) assay, both of Giemsa staining and flow cytometric analysis showed that the treatment of CEE increased the number of micronuclei and correlated with apoptotic induction results. Moreover, the combination of CEE and DOXO induced cells accumulation in Sub-G1 and G2/M phase. In conclusion, CEE performed antigenotoxic effect in an in vivo model and cytotoxic effect on CHO-K1 cells.
      PubDate: 2019-03-01
       
  • Gene expression profile of PM014 of immortalized mouse lung epithelial
           cells in response to the effect of PM014 on radiation-induced fibrosis
    • Abstract: Radiotherapy is a major method for cancer treatment, but it frequently causes various side effects such as radiation-induced pneumonia and pulmonary fibrosis. Drugs to treat these side effects are urgently needed in the clinic, since there is no clearly defined medication for treating these symptoms. Previous studies demonstrated that the herbal formula, PM014, is effective for radiation-induced lung injury and fibrosis in mice. In this study, we investigated gene expression profiles to understand the mechanism of action behind the effects of PM014 on radiation induced damage in immortalized lung epithelial cells, MLE12. We performed QuantSeq 3′ mRNA-Seq analysis on the mRNA from radiation treated MLE-12 cells in the presence and absence of PM014. Transcriptome analysis found that 217 genes were significantly affected by PM014. Among them (217 genes, > twofold, p value < 0.05, 4 normalize), 77 genes were found to be upregulated, and 140 genes were downregulated in response to PM014 treatment in a dose dependent manner. Using the Kyoto Encyclopedia of Genes and Genomes analysis, we found that genes involved in cytokine–cytokine receptor interaction pathways were the most strongly affected by PM014. Based on these data, we selected 20 genes, and performed real-time PCR. Expression of 11 genes, including IL-18, IL-12a, Tnfrsf9, IL-17, CCR5, Blnk, Irf8, Nrros, TGF-β, Relt, and Cxcl2 was increased after irradiation, while PM014 treatment showed the reversed expression pattern of these genes. Therefore, PM014 may be useful for the treatment of radiation induced lung injury by modulating genes involved in cytokine–cytokine receptor interaction pathway.
      PubDate: 2019-03-01
       
  • Bioactive extract of Artemisia judaica causes in vitro inhibition of
           dipeptidyl peptidase IV and pancreatic/intestinal enzymes of the
           carbohydrate absorption cascade: implication for anti-diabetic new
           molecular entities (NMEs)
    • Abstract: Artemisia judaica is used in traditional medicine in the Arabian Gulf to treat several ailments including diabetes. The present study evaluated the hydro-methanolic extract obtained from aerial parts of A. judaica (AJ-HA) for its potential to inhibit key blood sugar modulating enzymes in vitro and its antioxidant activity with phytochemical composition. AJ-HA was tested for in vitro hypoglycemic effect by its potential to inhibit pancreatic α-amylase, intestinal α-glucosidase and dipeptidyl peptidase IV (DPP IV). Antioxidant activity was determined by assessing the potential of the extract through scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Quantitative phytochemical evaluation was performed by determining the total content of phenolics, saponins, flavonoids, tannins and alkaloids. Interestingly the extract showed inhibitory potential for all the three key enzymes that are involved in modulating the blood glucose levels namely: α-amylase, α-glucosidase and DPP IV with IC50s in the range of 758.96–2447.40 µg/mL. AJ-HA also showed significant scavenging activity for DPPH radicals with IC50 of 85.89 µg/mL. Quantitative estimations confirmed the abundance of various phytochemical classes particularly saponins and tannins. As such, this is a proof of concept study wherein our results demonstrate that A. judaica which is used in traditional medicine for anti-diabetic properties contains active metabolites that have the potential to inhibit key blood sugar modulating enzymes. Our findings therefore has implication that warrants further investigation of A. judaica in animal models and further for the identification of lead compounds as new molecular entities for management of diabetes.
      PubDate: 2019-03-01
       
  • Phytochemical screening and evaluation of the pharmacological activities
           of ethanolic extract of Argemone mexicana Linn. aerial parts
    • Abstract: The present study was designed to investigate different phytochemical groups present in ethanolic extract of Argemone mexicana aerial parts (EAMA) as well as to assess the analgesic, antidiarrheal, antibacterial, anthelmintic and cytotoxic activities of EAMA. Moreover, peripheral and central analgesic activities were evaluated by acetic acid-induced writhing test, formalin-induced paw licking test, tail immersion test and hot plate test. In vivo castor oil-induced diarrheal model and magnesium sulphate induced diarrheal model in mice were utilized for the assessment of antidiarrheal activity. Again, antibacterial activity was evaluated using disc diffusion assay. Anthelmintic activity was carried out on Paramphistomum cervi (Trematoda). Cytotoxic activity was assessed through brine shrimp lethality bioassay. The extract demonstrated the presence of alkaloids, flavonoids, phenolic compounds, tannins, glycosides and gums in phytochemical screening. In case of acetic acid-induced writhing and formalin induced paw licking test, both lower and higher doses of EAMA showed significant percentage inhibition of writhing as well as paw licking respectively (*P < 0.05, vs. control). EAMA at the doses of 200 mg/kg and 400 mg/kg revealed significant latency response (*P < 0.05, vs. control) in delayed phase in both tail immersion as well as hot plate test. Moreover, significant percentage inhibition (*P < 0.05, vs. control) of diarrhea was exposed by both doses of EAMA in in vivo diarrheal models. In disc diffusion assay, EAMA showed antibacterial activities against both gram positive and gram negative bacterial strains. Again, the extract exhibited anthelmintic and cytotoxic activity in a dose dependent manner.
      PubDate: 2019-03-01
       
  • Comparative study of anti-allergic activity of two poly-herbal
           formulations in ova-challenged allergic rhinitis mice model
    • Abstract: This study was to prepare a novel in situ gel system for nasal delivery of poly-herbal extracts and study its efficacy on the allergic rhinitis model. The main purpose is to compare the efficacy of poly-herbal nasal drops and in situ gel. A temperature depended in situ gel was developed and characterized with gellan gum as a carrier. The system was stable kept at 40 ± 2 °C for 6 months, and the micrographic results showed that in situ gel was safety without mucous irritation when given daily for 26 days to mice with allergic rhinitis that the effect of drops for the treatment. Extracts in gellan gum produced obviously effect on allergic rhinitis at the doses of 20 g/body following intranasal administration, and the efficacy was significantly superior to that of the drops (p < 0.01). This study uses Kruskal–Wallis test analysis of variance (ANOVA) followed by Dunn’s test for the first time to develop, optimize the formulation and assess factors affecting the critical quality attributes. Histopathological study of the nasal mucosa suggested that the formulation was safe for nasal administration. The statistical difference in absolute bioavailability between drop and in situ gel by nasal route suggested that in situ gel had almost 21% increase in bioavailability for Moringa oleifera and for Embelia Ribes in combination there was a 16% increase over drop formulations. Thus the situ gel system is a promising approach for the intranasal delivery of polyherbal extracts for the therapeutic effect improvement. Graphical abstract
      PubDate: 2019-03-01
       
  • Developments in taxol production through endophytic fungal biotechnology:
           a review
    • Abstract: Paclitaxel (taxol), a diterpene natural compound was first extracted from the bark of yew trees. However, the method cannot meet its increasing demand on the market due to unprecedented yew cutting, low amounts of taxol production, the laborious and slow process of taxol extraction. Recently, efforts have been made to develop alternative means of taxol production. Microbial fermentation would be a promising method in the production of taxol at industrial scale. Fungal endophytes have the capacity to produce bioactive compounds and can independently synthesize secondary metabolites similar to those made by the host plants. Optimization of the fermentation culture is one of the most important strategies in increasing taxol production by endophytic fungi supplemented with several substances including carbon sources, nitrogen sources, precursors, inducer and the metabolic bypass inhibitors. Improved fermentation techniques and different biotechnological strategies such as gene cloning, gene transformation, mutations are widely being used on endophytic fungi in order to increase the productivity of the taxol. In this review, the different strategies used to produce taxol from endophytic fungal biotechnology have been discussed.
      PubDate: 2019-03-01
       
  • The Tibetan medicine compound Tsotel : safety and efficacy
    • Abstract: A class of complex Tibetan pills commonly called precious pills, or Rinchen rilbu in Tibetan, is a compounded Tibetan formula combining dozens of minerals and herbs. Tsotel (btso thal), the key medicinal compound in precious pills, synergizes with the other herbs and minerals in each formula and is used to treat strokes, inflammation, chemical poisoning, such as anthrax, neural disorders and diseases arising from various toxicities. Tibetans have used these medicines for at least 2000 years. However, since the medicinal compound of tsotel includes minerals such as gold, iron, and especially mercury, some scholars and physicians have toxicity concerns surrounding tsotel administration. Thus, elucidating the traditional process of compounding this medicinal substance will improve our understanding of tsotel’s safety and efficacy.
      PubDate: 2019-03-01
       
  • A comparative study on the estimated glycemic index (eGI), phenolic
           constituents, antioxidative and potential antihyperglycemic effects of
           different parts of ripe Citrus paradisi fruit
    • Abstract: This study compared the estimated glycemic index, antioxidant, and α-amylase and α-glucosidase inhibitory properties of ripe Citrus paradisi fruit’s parts, as well as their phenolic composition by employing high performance liquid chromatography fixed with diode array detector. The result revealed that C. paradisi fruit’s peel exhibited significantly lowest glycemic index, highest antioxidant and inhibitory effects on the activities of α-amylase and α-glucosidase, with concomitant highest polyphenol constituents compared to other parts of the fruit. Thus, being a waste product of the fruit, it could be a prospective source of natural products for functional food development, nutraceuticals or dietary supplement for the management of diabetes, due to the low glycemic index, antioxidant and inhibition of α-glucosidase and α-amylase activities.
      PubDate: 2019-03-01
       
  • Quantification of the secondary metabolites by HPTLC, analgesic and
           antipyretic activity evaluation of Ficus racemosa L. leaves
    • Abstract: There have been no reports of simultaneous quantification of kaempferol and quercetin in Ficus racemosa L. Objective of the present work included to perform extraction using petroleum ether (PEE) and ethanol (EE), to perform preliminary phytochemical test, quantitative estimation of phytochemicals and simultaneous estimation of kaempferol and quercetin in Ficus racemosa L. using high performance thin layer chromatography (HPTLC) method and to assess the analgesic and antipyretic activity of the extracts. PEE and EE obtained from cold maceration technique. Extracts were subjected to qualitative test and estimated quantitatively. Extracts were applied on silica gel G 60 F254 plate; solvent-Toluene: Chloroform: Acetone 4.5:4.5:1 (v/v) and scanned at 254 nm. The extracts were also subjected to analgesic activity (hot plate method and tail immersion method) and antipyretic (Brewer’s yeast induced pyrexia model and Lipopolysaccharide induced pyrexia mode) activity. Findings of preliminary phytochemical test and quantitative estimation of phytochemicals confirms the presence of flavonoids in both the extracts (PEE and EE). The amount of flavonoids in PEE and EE were found to be 24.58 ± 0.60 mg/100 g and 21.01 ± 0.58 mg/100 g respectively. HPTLC system resulted well resolved bands for quercetin (Rf 0.38) and kaempferol (Rf 0.55). The validated HPTLC method was found suitable for kaempferol and quercetin quantification in Ficus racemosa L. Hot plate reaction time response was improved in a dose-dependent manner in a group of rats treated with PEE suggesting its analgesic potential. In view of analgesic potential by tail immersion method, PEE (400 mg/kg b.w.) showed no significant difference in tail flick reaction time as shown by standard treated animal (Aspirin 100 mg/kg b.w.). The findings of antipyretic activity suggested that PEE (400 mg/kg b.w.) have no significant difference in treating pyrexia when compared with Paracetamol (100 mg/kg). PEE (400 mg/kg b.w.) was found to be most potent. It may be due to the presence of comparatively high amount of flavonoids in PEE confirmed by the preliminary phytochemical test, quantitative estimation of phytochemicals and HPTLC quantification the PEE was found to be more effective when compared to EE.
      PubDate: 2019-03-01
       
  • Evaluation of antitumor activity of Urena lobata against Ehrlich ascites
           carcinoma treated mice
    • Abstract: The search for cancer therapy with natural products continues to be a global effort. Many natural products have been tested globally against various cancer cell lines. Urena lobata Linn. (U. lobata) has been used as an ingredient in various formulations of traditional medicines for the treatment of cancers. The present study was undertaken to investigate the antitumor activity of the methanol (MEUL) and aqueous (AEUL) extracts of the U. lobata whole herb in Swiss Albino mice against Ehrlich ascites carcinoma (EAC) cell line. Both extracts were administered orally (200 mg/kg and 400 mg/kg) for 13 consecutive days. After 24 h of the last dose, the animals were scarified. The antitumor effect of the MEUL and AEUL was determined by evaluating the median survival time (days), %ILS, tumor volume, viable tumor cell count, non-viable tumor cell count and tumor weight (g) and hematological parameters of EAC-bearing mice. Both MEUL and AEUL showed significant decrease in tumor volume, the viable cell count, tumor weight, elevated mean survival time, %ILS and non-viable cell count. Hematological profile such as RBC, WBC, and haemoglobin count was reverted to normal level in treated mice. In conclusion, MEUL (400 mg/kg) showed prominent antitumor activity against EAC treated mice.
      PubDate: 2019-03-01
       
  • The effect of Elsholtzia densa methanolic extract modulates inflammation
           in vitro and in vivo
    • Abstract: Inflammatory diseases being one of the back bending global burden are alarming the world. Established anti-inflammatory drugs exert side-effects, demanding for the development of alternative drugs, likely from natural source because of their diverse chemical nature. The anti-inflammatory activity of aerial part methanol extract of Elsholtzia densa collected from the natural forest of Naranag area of Kashmir valley was evaluated both in vitro and in vivo model systems using Macrophages RAW 264.7 cells and acute (carrageenan and histamine-induced rat paw edema) and chronic model (formaldehyde induced arthritis) respectively. The EDM extract exhibited a significant decline in the paw volume being at par with the standard drug Diclofenac (10 mg/kg) as compared with the control group. The reduction was observed in dose dependent manner with 300 mg/kg resulting in 27.27% and 100 mg/kg in 16.88% inhibition at 3rd h. A statistically significant (p < 0.05) decline in the secretion of serum pro-inflammatory cytokines like TNF-α, IL-1 β and IL-6 was found with 80.93%, 75.33% and 67.09%, and 83.25%, 62.11% and 66.69% in EDM-300 as compared with the carrageenan and histamine group respectively. The pro-inflammatory cytokines levels showed clampdown in LPS stimulated RAW264.7 by the extract treatments. The LC–MS data revealed the presence of Quercetin 3-glycosides, Apigenin 7-(2″-acetyl-6″-methylglucuronide) and betulinic acid as major compounds present in EDM extract. Results obtained from in vivo experiments demonstrate that oral administration of EDM extract significantly attenuated inflammation in carrageenan and histamine-induced paw edema, and improved chronic models (formaldehyde induced arthritis).
      PubDate: 2019-03-01
       
  • Phytochemical profile, antioxidant and antimicrobial activities of
           Traganum nudatum Delile aerial parts organic extracts collected from
           Algerian Sahara’s flora
    • Abstract: The aim of this study was to investigate for the first time the antioxidant and the antimicrobial activities of organic extracts from Traganum nudatum Delile using in vitro models. The extracts which exhibited the highest activities were then submitted to RP-HPLC–PDA analysis. The phytochemical screening revealed the presence of flavonoids, tannins, alkaloids, saponins and quinones. The highest amounts of polyphenols, flavonoids and flavonols were recorded in ethyl acetate fractions. The strongest DPPH scavenging ability was attributed to ethyl acetate fraction of crude extract prepared by maceration EAFM with IC50 value of 20 ± 0.11 µg/mL, ethyl acetate fraction of crude extract prepared by infusion EAFI was found to possess the highest ferric reducing power with EC50 of 35 ± 0.4 µg/mL. The highest antioxidant capacity 200.21 ± 0.2 mg AAE/g was exhibited by n-butanol fraction of crude extract prepared by maceration BFM. The antimicrobial activity was proven against all the tested microorganisms except on Candida albicans strains. The extracts were more active on Gram positive than Gram negative bacteria. The strongest inhibition was provided against Bacillus subtilis, Bacillus cereus and Listeria monocytogenes. The identification of phenolic compounds using RP-HPLC–PDA technique revealed the presence of sinapic, syringic, ferulic, p-coumaric acids, catechin, naringenin and vanillin that may be responsible for the bioactivities of the reported specie. Our results indicate that T. nudatum Delile may provide a rich and novel source of natural antioxidants for industrial utilization replacing synthetic ones.
      PubDate: 2019-02-21
       
  • An updated literature-based review: phytochemistry, pharmacology and
           therapeutic promises of Nigella sativa L.
    • Abstract: An in depth review on Nigella sativa and its derived constituents has been necessitated which has been sketched in this paper from the research reports obtained from PubMed and ScienceDirect databases. Findings of this meticulous review suggest that N. sativa possesses various important phytoconstituents and derived compounds with diverse biological effects including antioxidant, anti-inflammatory, anti-bacterial, anti-fungal, anti-parasitic, anti-protozoal, antiviral, cytotoxic, anticancer, and neuro-, gastro-, cardio-, hepato- and nephro-protective activities. In addition, N. sativa implies beneficiary effects on reproductive, pulmonary and immune systems along with diabetes mellitus (type 2 diabetes), fertility, breast cancer, dermatological complications, dehydration, dyspepsia, osmotic balance and others. Amongst several isolated chemical moieties of N. sativa, thymoquinone may be one of the best targets for treatment of microbial infections, inflammations, cancer, metabolic syndromes, and many other diseases. The N. sativa is evident to promote health in some non-clinical and clinical studies and it may serve to be one of the best sources for modern phyto medicine.
      PubDate: 2019-02-21
       
  • The aqueous extract of Allium saralicum R.M. Fritsch effectively treat
           induced anemia: experimental study on Wistar rats
    • Abstract: There are many medicinal plants in traditional medicine which are used to prevent, control, and treat anemia. One of these plants is Allium saralicum R.M. Fritsch. The purpose of our research was to investigate the effect of aqueous extract of A. saralicum leaf in the treatment of hemolytic anemia. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of Phenylhydrazine in 50 animals. Then, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. saralicum at 25, 50, 100, and 200 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were weight and then sacrificed. The blood, liver and spleen samples were drawn immediately to analyze the hematological, biochemical and histological parameters. All groups of A. saralicum (especially AS200) significantly (p ≤ 0.05) reduced the raised concentrations of Fe, ferritin, erythropoietin, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, and creatinine and increased the levels of body weight, HDL, total protein, albumin, WBC, lymphocyte, monocytes, platelet, RBC, Hb, PCV, MCV, MCH, and MCHC as compared to the untreated group. Also, A. saralicum at all doses prevented pathological changes in the liver and spleen. In conclusion, because of aqueous extract of A. saralicum leaf anti-anemic property, it can be used as a medical supplement or drug.
      PubDate: 2019-02-14
       
  • Ethnopharmacological survey on traditional medicinal plants at Keraniganj,
           Dhaka, Bangladesh
    • Abstract: The conventional source of medicinal plants is an important way for daily curative uses in rural area throughout Bangladesh. Folk medicinal specialists or Kavirajes, assume an indispensable part in a medicinal services arrangement of both provincial and urban populace of the nation. It is vital to conduct broad meetings of individual Kavirajes of an area to get the overall status of medicinal plants. A survey was accomplished in the rustic territory of Atibazaar, Keraniganj Upazila, Dhaka, Bangladesh. This investigation has uncovered a sum of 38 plants out of which 25 plants were identified, which belongs to 21 families. Whole plants and/or plant parts, like leaves, stems, barks, roots, flowers, fruits, seeds and wood were usually used by Kavirajes for the treatment of different illnesses. Leaves (84%) were the most oftentimes used plant parts, trailed by roots (52%), fruits (16%) stems and barks (16%), entire plants (12%), seeds (12%), blossoms (8%) and wood (4%). Add up to 30 diverse individual illnesses professed to be restored by plant parts including respiratory tract disorders (asthma, cough, whooping cough, bronchitis), fever, gastrointestinal disorders (constipation, diarrhea, abdominal pain, blood dysentery, flatulence), arthritis, pain, headache, irregular menstruation, piles, tooth infections, whitening teeth, diabetes, skin diseases, acne, insect, animals and reptiles bites, urinary tract disorders, inflammation and nervousness. Several uses of the plants could be validated by available literature review. It is expected that other plants used for treatment of various diseases by the Kavirajes can be subjected for further bio-activity and phytochemical studies, which prompts disclosure of new medications.
      PubDate: 2019-02-05
       
  • Cytogenotoxic study of aqueous fruit extract of Morinda citrifolia in
           Wistar albino rats
    • Abstract: The use of herbal products and/or their derivatives as an alternative or complementary medicine becomes globally popular. To date, various biological activities have been reported in Morinda citrifolia Linn, especially antioxidants, antimicrobial, anti-inflammatory and antitumor effects. This study aimed to investigate the genotoxicity and DNA repair capacity of the aqueous fruit extract of M. citrifolia (AEMC). Additionally, we checked the mutagenic capacity of AEMC by the comet assay and micronucleus tests in rats. For this, rats (n = 10; 5 males and 5 females) were divided into five groups as: negative control (distilled water); positive control (25 mg/kg of cyclophosphamide, i.p.); and three groups for AEMC (2.5, 5 and 10 mg/kg, i.p.). The results suggest that, the AEMC induced genotoxicity in white blood cells, but a DNA repair capacity was also observed after 72 h of AEMC exposure. AEMC also showed cytotoxicity and mutagenicity in liver and kidney cells of rats at 5 and 10 mg/kg doses. In conclusion, AEMC exhibited a dose-dependent cytotoxicity, genotoxicity and mutagenicity along with a time-dependent DNA repair capacity in rats. Further studies are necessary regarding the safe use of this medicinal plant for human consumption.
      PubDate: 2019-01-23
       
  • Evaluation of aflatoxin contamination in crude medicinal plants used for
           the preparation of herbal medicine
    • Abstract: The medical focus is now on phytofoods, drugs and the consumption of medicinal plants is expanding worldwide. Safety and quality of herbal preparations is of great concern. Quality determines reproducible efficacy of herbal drugs and also safety is a concern both for public and health authorities in many countries specially developing ones. The reason attributed is that many contaminants and microbes that may cause quality deterioration and directly harm to the consumers, find entry in the crude herbal materials during collection and storage. The safety of these products is partially compromised due to the microbial presence, especially toxigenic fungi. The present study was designed to investigate the microbiological quality after storage of locally sold raw medicinal plants that were supplied to different pharma units involved in the preparation of herbal and various formulations of ayurvedic drugs. Twenty samples of raw medicinal plants were stored at room temperature for a year and subjected to microbiological evaluation and found that most of the samples loaded with bacterial and fungal contents, do not comply with the FDA regulations. The presence of aflatoxin gave signals of aflatoxin producing fungi i.e. A. flavus and A. parasiticus. So, there is an urgent need of making strategy to control the microbes during pre-harvest and post-harvest procedures. This study is an attempt to emphasize the need for consistent quality assessment of crude herbal drugs for safe therapeutic products suitable for human beings. Our findings may help in instituting public health standards towards production and safety of herbal drugs worldwide.
      PubDate: 2018-12-18
       
  • Inhibitory effect of effective fraction of Salvia officinalis on aldose
           reductase activity: strategy to reduce complications of type 2 diabetes
    • Abstract: Aldose reductase (AR) is the key enzyme of the polyol pathway, which plays an important role in the pathogenesis of diabetic complications. AR inhibitors can be used as an important strategy in the treatment of diabetic complications. The aim of the present study was to investigate effect of different fractions of Salvia officinalis on the bovine lens aldose reductase activity. For this purpose, the phenolic and flavonoid contents, IC50 values of different fractions of the S. officinalis to neutralize the DPPH free radicals were first measured. Then, attempts were made to investigate the effect of these fractions on the AR enzyme activity. Results indicated that ethyl acetate fraction had the highest of phenolic and flavonoid contents by 412.6 ± 1.55 and 372.5 ± 6.47 mg/ml, respectively. Also, the ethyl acetate fraction showed the lowest IC50 content of 1.18 μg/ml for scavenging of the free radicals and 9.25 μg/ml for the inhibition of AR activity. According to the Lineweaver–Burk plot, the ethyl acetate fraction acts as an uncompetitive enzyme inhibitor. These findings revealed that all fractions showed inhibitory effect on AR activity, where in ethyl acetate fraction it was found to be maximum which may be due to its high phenolic and flavonoid content.
      PubDate: 2018-12-06
       
 
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